zgc:101731 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	zgc:101731
Entrez Gene ID	447892
Full Name	zgc:101731
Synonyms	fj55h11,snap25,wu:fj55h11
General protein information	Preferred Names zgc:101731 Names synaptosomal-associated protein 25
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005157731.3	XP_005157788.1	synaptosomal-associated protein 25 isoform X2
XM_005157730.3	XP_005157787.1	synaptosomal-associated protein 25 isoform X1
XM_005157730.4	XP_005157787.1	synaptosomal-associated protein 25 isoform X1
XM_005157732.4	XP_005157789.1	synaptosomal-associated protein 25 isoform X3
NM_001025558.2	NP_001020729.1	synaptosomal-associated protein 25
XM_005157732.3	XP_005157789.1	synaptosomal-associated protein 25 isoform X3
XM_005157731.4	XP_005157788.1	synaptosomal-associated protein 25 isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100491227	XP_002938631.1
Danio rerio (zebrafish)	zgc:101731	NP_001020729.1
Drosophila melanogaster (fruit fly)	Snap25	NP_001036642.2
Caenorhabditis elegans (roundworm)	ric-4	NP_505641.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001394	XP_321742.4

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Recruitment and SNARE-mediated fusion of vesicles in furrow membrane remodeling during cytokinesis in zebrafish embryos.
Li WM, Webb SE, Lee KW, Miller AL
Experimental cell research312(17)3260-75(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data indicate that SNARE proteins VAMP-2 and SNAP-25 play an essential role in daughter blastomere apposition, possibly via the delivery of components that promote the cell-to-cell adhesion required for the successful completion of cytokinesis
Title: Recruitment and SNARE-mediated fusion of vesicles in furrow membrane remodeling during cytokinesis in zebrafish embryos.

The following zgc:101731 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zgc:101731 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa20593	NM_001025558.2 Latest version!	Danio rerio zgc:101731 (zgc:101731), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa20593	XM_005157730.4 Latest version!	Danio rerio zgc:101731 (zgc:101731), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa20594	XM_005157731.4 Latest version!	Danio rerio zgc:101731 (zgc:101731), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa20595	XM_005157732.4 Latest version!	Danio rerio zgc:101731 (zgc:101731), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
ODa20593	XM_005157730.3	Danio rerio zgc:101731 (zgc:101731), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa20595	XM_005157732.3	Danio rerio zgc:101731 (zgc:101731), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa20594	XM_005157731.3	Danio rerio zgc:101731 (zgc:101731), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

zgc:101731 ( NM_001025558.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001020729.1 Danio rerio zgc:101731 (zgc:101731), mRNA.

Price & Availability↑

CloneID	ODa20593
Clone ID Related Accession (Same CDS sequence)	XM_005157730.3 , XM_005157730.4 , NM_001025558.2
Accession Version	NM_001025558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	synaptosomal-associated protein 25
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX004776.4. On or before May 1, 2009 this sequence version replaced NM_001004631.2, NM_001025558.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC096970.1, CN019372.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCTACCG ACTCCACCAT GAGATCCGAA GTGGAGGAGT TACAGAAGAA GGCCAATCAG 
ATGACTGATG AGTCTCTTGA GGTCACCAGA AACATGAAGC TTCTATTGGA GGAGAGTAAA 
GATGCGGGCA TCAGGACACT TGTTATGCTG GACGAGCAGG GAGAACAACT GGACCGCATA 
GAAGAGGGCT TGGATCAGAT CAACAAGGAC ATGAAGGAGG CTGAGAAAAA TCTGACTGAC 
ATGGCCATGT GTTGCGGTCT GTGCATCTGG CCATGTACAA AATTAAAGGA CTTTGAAGCA 
AGTGGAGCAT ATAAGAAGGT GTGGGGTAAT AATCAAGATG GAGTGGTGTC GAGCCAGCCA 
TCTTCTCGCG TGGTGGATGA GCGAGAGAAG ATGACCATGA GCGGTGGATA TATAAGAAGA 
GTGACAAATG ATGCAAGGGA GGATGAAATG GAGGAGAATT TGGCCCAGGT TGGCAGTATC 
CTTGGCAACC TCAGAAGCAT GGCACTCGAC ATGGGAAATG AAATCGACAC CCAGAACGTC 
CAGATTGAAC GCATACAGAG CAAGGCCGTG GTCAATGAAT CACGCATCAA CGAGGCCAAC 
CAGAGAGCCA CAAAGCTAAT ATCAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020729.1
CDS	144..773
Translation	MATDSTMRSEVEELQKKANQMTDESLEVTRNMKLLLEESKDAGIRTLVMLDEQGEQLDRIEEGLDQINKDMKEAEKNLTDMAMCCGLCIWPCTKLKDFEASGAYKKVWGNNQDGVVSSQPSSRVVDEREKMTMSGGYIRRVTNDAREDEMEENLAQVGSILGNLRSMALDMGNEIDTQNVQIERIQSKAVVNESRINEANQRATKLISR

Target ORF information:

RefSeq Version	NM_001025558.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:101731 (zgc:101731), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025558.2

ORF Insert Sequence:

ATGGCTACCG ACTCCACCAT GAGATCCGAA GTGGAGGAGT TACAGAAGAA GGCCAATCAG 
ATGACTGATG AGTCTCTTGA GGTCACCAGA AACATGAAGC TTCTATTGGA GGAGAGTAAA 
GATGCGGGCA TCAGGACACT TGTTATGCTG GACGAGCAGG GAGAACAACT GGACCGCATA 
GAAGAGGGCT TGGATCAGAT CAACAAGGAC ATGAAGGAGG CTGAGAAAAA TCTGACTGAC 
ATGGCCATGT GTTGCGGTCT GTGCATCTGG CCATGTACAA AATTAAAGGA CTTTGAAGCA 
AGTGGAGCAT ATAAGAAGGT GTGGGGTAAT AATCAAGATG GAGTGGTGTC GAGCCAGCCA 
TCTTCTCGCG TGGTGGATGA GCGAGAGAAG ATGACCATGA GCGGTGGATA TATAAGAAGA 
GTGACAAATG ATGCAAGGGA GGATGAAATG GAGGAGAATT TGGCCCAGGT TGGCAGTATC 
CTTGGCAACC TCAGAAGCAT GGCACTCGAC ATGGGAAATG AAATCGACAC CCAGAACGTC 
CAGATTGAAC GCATACAGAG CAAGGCCGTG GTCAATGAAT CACGCATCAA CGAGGCCAAC 
CAGAGAGCCA CAAAGCTAAT ATCAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:101731 ( XM_005157730.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157787.1 Danio rerio zgc:101731 (zgc:101731), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20593
Clone ID Related Accession (Same CDS sequence)	XM_005157730.3 , XM_005157730.4 , NM_001025558.2
Accession Version	XM_005157730.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptosomal-associated protein 25 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157730.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCG ACTCCACCAT GAGATCCGAA GTGGAGGAGT TACAGAAGAA GGCCAATCAG 
ATGACTGATG AGTCTCTTGA GGTCACCAGA AACATGAAGC TTCTATTGGA GGAGAGTAAA 
GATGCGGGCA TCAGGACACT TGTTATGCTG GACGAGCAGG GAGAACAACT GGACCGCATA 
GAAGAGGGCT TGGATCAGAT CAACAAGGAC ATGAAGGAGG CTGAGAAAAA TCTGACTGAC 
ATGGCCATGT GTTGCGGTCT GTGCATCTGG CCATGTACAA AATTAAAGGA CTTTGAAGCA 
AGTGGAGCAT ATAAGAAGGT GTGGGGTAAT AATCAAGATG GAGTGGTGTC GAGCCAGCCA 
TCTTCTCGCG TGGTGGATGA GCGAGAGAAG ATGACCATGA GCGGTGGATA TATAAGAAGA 
GTGACAAATG ATGCAAGGGA GGATGAAATG GAGGAGAATT TGGCCCAGGT TGGCAGTATC 
CTTGGCAACC TCAGAAGCAT GGCACTCGAC ATGGGAAATG AAATCGACAC CCAGAACGTC 
CAGATTGAAC GCATACAGAG CAAGGCCGTG GTCAATGAAT CACGCATCAA CGAGGCCAAC 
CAGAGAGCCA CAAAGCTAAT ATCAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157787.1
CDS	148..777
Translation	MATDSTMRSEVEELQKKANQMTDESLEVTRNMKLLLEESKDAGIRTLVMLDEQGEQLDRIEEGLDQINKDMKEAEKNLTDMAMCCGLCIWPCTKLKDFEASGAYKKVWGNNQDGVVSSQPSSRVVDEREKMTMSGGYIRRVTNDAREDEMEENLAQVGSILGNLRSMALDMGNEIDTQNVQIERIQSKAVVNESRINEANQRATKLISR

Target ORF information:

RefSeq Version	XM_005157730.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zgc:101731 (zgc:101731), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157730.3

ORF Insert Sequence:

ATGGCTACCG ACTCCACCAT GAGATCCGAA GTGGAGGAGT TACAGAAGAA GGCCAATCAG 
ATGACTGATG AGTCTCTTGA GGTCACCAGA AACATGAAGC TTCTATTGGA GGAGAGTAAA 
GATGCGGGCA TCAGGACACT TGTTATGCTG GACGAGCAGG GAGAACAACT GGACCGCATA 
GAAGAGGGCT TGGATCAGAT CAACAAGGAC ATGAAGGAGG CTGAGAAAAA TCTGACTGAC 
ATGGCCATGT GTTGCGGTCT GTGCATCTGG CCATGTACAA AATTAAAGGA CTTTGAAGCA 
AGTGGAGCAT ATAAGAAGGT GTGGGGTAAT AATCAAGATG GAGTGGTGTC GAGCCAGCCA 
TCTTCTCGCG TGGTGGATGA GCGAGAGAAG ATGACCATGA GCGGTGGATA TATAAGAAGA 
GTGACAAATG ATGCAAGGGA GGATGAAATG GAGGAGAATT TGGCCCAGGT TGGCAGTATC 
CTTGGCAACC TCAGAAGCAT GGCACTCGAC ATGGGAAATG AAATCGACAC CCAGAACGTC 
CAGATTGAAC GCATACAGAG CAAGGCCGTG GTCAATGAAT CACGCATCAA CGAGGCCAAC 
CAGAGAGCCA CAAAGCTAAT ATCAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:101731 ( XM_005157730.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157787.1 Danio rerio zgc:101731 (zgc:101731), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20593
Clone ID Related Accession (Same CDS sequence)	XM_005157730.3 , XM_005157730.4 , NM_001025558.2
Accession Version	XM_005157730.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptosomal-associated protein 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157730.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCG ACTCCACCAT GAGATCCGAA GTGGAGGAGT TACAGAAGAA GGCCAATCAG 
ATGACTGATG AGTCTCTTGA GGTCACCAGA AACATGAAGC TTCTATTGGA GGAGAGTAAA 
GATGCGGGCA TCAGGACACT TGTTATGCTG GACGAGCAGG GAGAACAACT GGACCGCATA 
GAAGAGGGCT TGGATCAGAT CAACAAGGAC ATGAAGGAGG CTGAGAAAAA TCTGACTGAC 
ATGGCCATGT GTTGCGGTCT GTGCATCTGG CCATGTACAA AATTAAAGGA CTTTGAAGCA 
AGTGGAGCAT ATAAGAAGGT GTGGGGTAAT AATCAAGATG GAGTGGTGTC GAGCCAGCCA 
TCTTCTCGCG TGGTGGATGA GCGAGAGAAG ATGACCATGA GCGGTGGATA TATAAGAAGA 
GTGACAAATG ATGCAAGGGA GGATGAAATG GAGGAGAATT TGGCCCAGGT TGGCAGTATC 
CTTGGCAACC TCAGAAGCAT GGCACTCGAC ATGGGAAATG AAATCGACAC CCAGAACGTC 
CAGATTGAAC GCATACAGAG CAAGGCCGTG GTCAATGAAT CACGCATCAA CGAGGCCAAC 
CAGAGAGCCA CAAAGCTAAT ATCAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157787.1
CDS	147..776
Translation	MATDSTMRSEVEELQKKANQMTDESLEVTRNMKLLLEESKDAGIRTLVMLDEQGEQLDRIEEGLDQINKDMKEAEKNLTDMAMCCGLCIWPCTKLKDFEASGAYKKVWGNNQDGVVSSQPSSRVVDEREKMTMSGGYIRRVTNDAREDEMEENLAQVGSILGNLRSMALDMGNEIDTQNVQIERIQSKAVVNESRINEANQRATKLISR

Target ORF information:

RefSeq Version	XM_005157730.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:101731 (zgc:101731), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157730.4

ORF Insert Sequence:

ATGGCTACCG ACTCCACCAT GAGATCCGAA GTGGAGGAGT TACAGAAGAA GGCCAATCAG 
ATGACTGATG AGTCTCTTGA GGTCACCAGA AACATGAAGC TTCTATTGGA GGAGAGTAAA 
GATGCGGGCA TCAGGACACT TGTTATGCTG GACGAGCAGG GAGAACAACT GGACCGCATA 
GAAGAGGGCT TGGATCAGAT CAACAAGGAC ATGAAGGAGG CTGAGAAAAA TCTGACTGAC 
ATGGCCATGT GTTGCGGTCT GTGCATCTGG CCATGTACAA AATTAAAGGA CTTTGAAGCA 
AGTGGAGCAT ATAAGAAGGT GTGGGGTAAT AATCAAGATG GAGTGGTGTC GAGCCAGCCA 
TCTTCTCGCG TGGTGGATGA GCGAGAGAAG ATGACCATGA GCGGTGGATA TATAAGAAGA 
GTGACAAATG ATGCAAGGGA GGATGAAATG GAGGAGAATT TGGCCCAGGT TGGCAGTATC 
CTTGGCAACC TCAGAAGCAT GGCACTCGAC ATGGGAAATG AAATCGACAC CCAGAACGTC 
CAGATTGAAC GCATACAGAG CAAGGCCGTG GTCAATGAAT CACGCATCAA CGAGGCCAAC 
CAGAGAGCCA CAAAGCTAAT ATCAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:101731 ( XM_005157731.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157788.1 Danio rerio zgc:101731 (zgc:101731), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa20594
Clone ID Related Accession (Same CDS sequence)	XM_005157731.3 , XM_005157731.4
Accession Version	XM_005157731.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptosomal-associated protein 25 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCG ACTCCACCAT GAGATCCGAA GTGGAGGAGT TACAGAAGAA GGCCAATCAG 
ATGACTGATG AGTCTCTTGA GGTCACCAGA AACATGAAGC TTCTATTGGA GGAGAGTAAA 
GATGCGGGCA TCAGGACACT TGTTATGCTG GACGAGCAGG GAGAGCAACT GGAGCGGATT 
GAGGAAGGAC TGGACCAGAT CAACCAGGAC ATGAGGGAAG CTGAGAAAAA CCTTACAGAT 
CTGGGCAAAT GCTGTGGTCT GTGCTCCTGT GATAAATTAA AGGACTTTGA AGCAAGTGGA 
GCATATAAGA AGGTGTGGGG TAATAATCAA GATGGAGTGG TGTCGAGCCA GCCATCTTCT 
CGCGTGGTGG ATGAGCGAGA GAAGATGACC ATGAGCGGTG GATATATAAG AAGAGTGACA 
AATGATGCAA GGGAGGATGA AATGGAGGAG AATTTGGCCC AGGTTGGCAG TATCCTTGGC 
AACCTCAGAA GCATGGCACT CGACATGGGA AATGAAATCG ACACCCAGAA CGTCCAGATT 
GAACGCATAC AGAGCAAGGC CGTGGTCAAT GAATCACGCA TCAACGAGGC CAACCAGAGA 
GCCACAAAGC TAATATCAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157788.1
CDS	149..772
Translation	MATDSTMRSEVEELQKKANQMTDESLEVTRNMKLLLEESKDAGIRTLVMLDEQGEQLERIEEGLDQINQDMREAEKNLTDLGKCCGLCSCDKLKDFEASGAYKKVWGNNQDGVVSSQPSSRVVDEREKMTMSGGYIRRVTNDAREDEMEENLAQVGSILGNLRSMALDMGNEIDTQNVQIERIQSKAVVNESRINEANQRATKLISR

Target ORF information:

RefSeq Version	XM_005157731.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zgc:101731 (zgc:101731), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157731.3

ORF Insert Sequence:

ATGGCTACCG ACTCCACCAT GAGATCCGAA GTGGAGGAGT TACAGAAGAA GGCCAATCAG 
ATGACTGATG AGTCTCTTGA GGTCACCAGA AACATGAAGC TTCTATTGGA GGAGAGTAAA 
GATGCGGGCA TCAGGACACT TGTTATGCTG GACGAGCAGG GAGAGCAACT GGAGCGGATT 
GAGGAAGGAC TGGACCAGAT CAACCAGGAC ATGAGGGAAG CTGAGAAAAA CCTTACAGAT 
CTGGGCAAAT GCTGTGGTCT GTGCTCCTGT GATAAATTAA AGGACTTTGA AGCAAGTGGA 
GCATATAAGA AGGTGTGGGG TAATAATCAA GATGGAGTGG TGTCGAGCCA GCCATCTTCT 
CGCGTGGTGG ATGAGCGAGA GAAGATGACC ATGAGCGGTG GATATATAAG AAGAGTGACA 
AATGATGCAA GGGAGGATGA AATGGAGGAG AATTTGGCCC AGGTTGGCAG TATCCTTGGC 
AACCTCAGAA GCATGGCACT CGACATGGGA AATGAAATCG ACACCCAGAA CGTCCAGATT 
GAACGCATAC AGAGCAAGGC CGTGGTCAAT GAATCACGCA TCAACGAGGC CAACCAGAGA 
GCCACAAAGC TAATATCAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:101731 ( XM_005157731.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157788.1 Danio rerio zgc:101731 (zgc:101731), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa20594
Clone ID Related Accession (Same CDS sequence)	XM_005157731.3 , XM_005157731.4
Accession Version	XM_005157731.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptosomal-associated protein 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157731.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCG ACTCCACCAT GAGATCCGAA GTGGAGGAGT TACAGAAGAA GGCCAATCAG 
ATGACTGATG AGTCTCTTGA GGTCACCAGA AACATGAAGC TTCTATTGGA GGAGAGTAAA 
GATGCGGGCA TCAGGACACT TGTTATGCTG GACGAGCAGG GAGAGCAACT GGAGCGGATT 
GAGGAAGGAC TGGACCAGAT CAACCAGGAC ATGAGGGAAG CTGAGAAAAA CCTTACAGAT 
CTGGGCAAAT GCTGTGGTCT GTGCTCCTGT GATAAATTAA AGGACTTTGA AGCAAGTGGA 
GCATATAAGA AGGTGTGGGG TAATAATCAA GATGGAGTGG TGTCGAGCCA GCCATCTTCT 
CGCGTGGTGG ATGAGCGAGA GAAGATGACC ATGAGCGGTG GATATATAAG AAGAGTGACA 
AATGATGCAA GGGAGGATGA AATGGAGGAG AATTTGGCCC AGGTTGGCAG TATCCTTGGC 
AACCTCAGAA GCATGGCACT CGACATGGGA AATGAAATCG ACACCCAGAA CGTCCAGATT 
GAACGCATAC AGAGCAAGGC CGTGGTCAAT GAATCACGCA TCAACGAGGC CAACCAGAGA 
GCCACAAAGC TAATATCAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157788.1
CDS	148..771
Translation	MATDSTMRSEVEELQKKANQMTDESLEVTRNMKLLLEESKDAGIRTLVMLDEQGEQLERIEEGLDQINQDMREAEKNLTDLGKCCGLCSCDKLKDFEASGAYKKVWGNNQDGVVSSQPSSRVVDEREKMTMSGGYIRRVTNDAREDEMEENLAQVGSILGNLRSMALDMGNEIDTQNVQIERIQSKAVVNESRINEANQRATKLISR

Target ORF information:

RefSeq Version	XM_005157731.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:101731 (zgc:101731), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157731.4

ORF Insert Sequence:

ATGGCTACCG ACTCCACCAT GAGATCCGAA GTGGAGGAGT TACAGAAGAA GGCCAATCAG 
ATGACTGATG AGTCTCTTGA GGTCACCAGA AACATGAAGC TTCTATTGGA GGAGAGTAAA 
GATGCGGGCA TCAGGACACT TGTTATGCTG GACGAGCAGG GAGAGCAACT GGAGCGGATT 
GAGGAAGGAC TGGACCAGAT CAACCAGGAC ATGAGGGAAG CTGAGAAAAA CCTTACAGAT 
CTGGGCAAAT GCTGTGGTCT GTGCTCCTGT GATAAATTAA AGGACTTTGA AGCAAGTGGA 
GCATATAAGA AGGTGTGGGG TAATAATCAA GATGGAGTGG TGTCGAGCCA GCCATCTTCT 
CGCGTGGTGG ATGAGCGAGA GAAGATGACC ATGAGCGGTG GATATATAAG AAGAGTGACA 
AATGATGCAA GGGAGGATGA AATGGAGGAG AATTTGGCCC AGGTTGGCAG TATCCTTGGC 
AACCTCAGAA GCATGGCACT CGACATGGGA AATGAAATCG ACACCCAGAA CGTCCAGATT 
GAACGCATAC AGAGCAAGGC CGTGGTCAAT GAATCACGCA TCAACGAGGC CAACCAGAGA 
GCCACAAAGC TAATATCAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:101731 ( XM_005157732.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157789.1 Danio rerio zgc:101731 (zgc:101731), transcript variant X3, mRNA.

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CloneID	ODa20595
Clone ID Related Accession (Same CDS sequence)	XM_005157732.4 , XM_005157732.3
Accession Version	XM_005157732.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synaptosomal-associated protein 25 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157732.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAAG CTGAGAAAAA CCTTACAGAT CTGGGCAAAT GCTGTGGTCT GTGCTCCTGT 
GATAAATTAA AGGACTTTGA AGCAAGTGGA GCATATAAGA AGGTGTGGGG TAATAATCAA 
GATGGAGTGG TGTCGAGCCA GCCATCTTCT CGCGTGGTGG ATGAGCGAGA GAAGATGACC 
ATGAGCGGTG GATATATAAG AAGAGTGACA AATGATGCAA GGGAGGATGA AATGGAGGAG 
AATTTGGCCC AGGTTGGCAG TATCCTTGGC AACCTCAGAA GCATGGCACT CGACATGGGA 
AATGAAATCG ACACCCAGAA CGTCCAGATT GAACGCATAC AGAGCAAGGC CGTGGTCAAT 
GAATCACGCA TCAACGAGGC CAACCAGAGA GCCACAAAGC TAATATCAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157789.1
CDS	471..884
Translation	MREAEKNLTDLGKCCGLCSCDKLKDFEASGAYKKVWGNNQDGVVSSQPSSRVVDEREKMTMSGGYIRRVTNDAREDEMEENLAQVGSILGNLRSMALDMGNEIDTQNVQIERIQSKAVVNESRINEANQRATKLISR

Target ORF information:

RefSeq Version	XM_005157732.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:101731 (zgc:101731), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157732.4

ORF Insert Sequence:

ATGAGGGAAG CTGAGAAAAA CCTTACAGAT CTGGGCAAAT GCTGTGGTCT GTGCTCCTGT 
GATAAATTAA AGGACTTTGA AGCAAGTGGA GCATATAAGA AGGTGTGGGG TAATAATCAA 
GATGGAGTGG TGTCGAGCCA GCCATCTTCT CGCGTGGTGG ATGAGCGAGA GAAGATGACC 
ATGAGCGGTG GATATATAAG AAGAGTGACA AATGATGCAA GGGAGGATGA AATGGAGGAG 
AATTTGGCCC AGGTTGGCAG TATCCTTGGC AACCTCAGAA GCATGGCACT CGACATGGGA 
AATGAAATCG ACACCCAGAA CGTCCAGATT GAACGCATAC AGAGCAAGGC CGTGGTCAAT 
GAATCACGCA TCAACGAGGC CAACCAGAGA GCCACAAAGC TAATATCAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:101731 ( XM_005157732.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157789.1 Danio rerio zgc:101731 (zgc:101731), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa20595
Clone ID Related Accession (Same CDS sequence)	XM_005157732.4 , XM_005157732.3
Accession Version	XM_005157732.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synaptosomal-associated protein 25 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157732.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAAG CTGAGAAAAA CCTTACAGAT CTGGGCAAAT GCTGTGGTCT GTGCTCCTGT 
GATAAATTAA AGGACTTTGA AGCAAGTGGA GCATATAAGA AGGTGTGGGG TAATAATCAA 
GATGGAGTGG TGTCGAGCCA GCCATCTTCT CGCGTGGTGG ATGAGCGAGA GAAGATGACC 
ATGAGCGGTG GATATATAAG AAGAGTGACA AATGATGCAA GGGAGGATGA AATGGAGGAG 
AATTTGGCCC AGGTTGGCAG TATCCTTGGC AACCTCAGAA GCATGGCACT CGACATGGGA 
AATGAAATCG ACACCCAGAA CGTCCAGATT GAACGCATAC AGAGCAAGGC CGTGGTCAAT 
GAATCACGCA TCAACGAGGC CAACCAGAGA GCCACAAAGC TAATATCAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157789.1
CDS	472..885
Translation	MREAEKNLTDLGKCCGLCSCDKLKDFEASGAYKKVWGNNQDGVVSSQPSSRVVDEREKMTMSGGYIRRVTNDAREDEMEENLAQVGSILGNLRSMALDMGNEIDTQNVQIERIQSKAVVNESRINEANQRATKLISR

Target ORF information:

RefSeq Version	XM_005157732.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zgc:101731 (zgc:101731), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157732.3

ORF Insert Sequence:

ATGAGGGAAG CTGAGAAAAA CCTTACAGAT CTGGGCAAAT GCTGTGGTCT GTGCTCCTGT 
GATAAATTAA AGGACTTTGA AGCAAGTGGA GCATATAAGA AGGTGTGGGG TAATAATCAA 
GATGGAGTGG TGTCGAGCCA GCCATCTTCT CGCGTGGTGG ATGAGCGAGA GAAGATGACC 
ATGAGCGGTG GATATATAAG AAGAGTGACA AATGATGCAA GGGAGGATGA AATGGAGGAG 
AATTTGGCCC AGGTTGGCAG TATCCTTGGC AACCTCAGAA GCATGGCACT CGACATGGGA 
AATGAAATCG ACACCCAGAA CGTCCAGATT GAACGCATAC AGAGCAAGGC CGTGGTCAAT 
GAATCACGCA TCAACGAGGC CAACCAGAGA GCCACAAAGC TAATATCAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Recruitment and SNARE-mediated fusion of vesicles in furrow membrane remodeling during cytokinesis in zebrafish embryos.
Experimental cell research312(17)3260-75(2006 Oct)
Li WM,Webb SE,Lee KW,Miller AL

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zgc:101731 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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