uhrf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	uhrf1
Entrez Gene ID	406350
Full Name	ubiquitin-like with PHD and ring finger domains 1
Synonyms	NP95,fb97f09,wu:fb97f09,zgc:63539
General protein information	Preferred Names ubiquitin-like with PHD and ring finger domains 1 Names E3 ubiquitin-protein ligase UHRF1 RING-type E3 ubiquitin transferase UHRF1 ubiquitin-like PHD and RING finger domain-containing protein 1 ubiquitin-like, containing PHD and RING finger domains, 1 ubiquitin-like-containing PHD and RING finger domains protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213077.1	NP_998242.1	E3 ubiquitin-protein ligase UHRF1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

UHRF1 regulation of Dnmt1 is required for pre-gastrula zebrafish development.
Kent B, Magnani E, Walsh MJ, Sadler KC
Developmental biology412(1)99-113(2016 Apr)

Epigenetic control of intestinal barrier function and inflammation in zebrafish.
Marjoram L, Alvers A, Deerhake ME, Bagwell J, Mankiewicz J, Cocchiaro JL, Beerman RW, Willer J, Sumigray KD, Katsanis N, Tobin DM, Rawls JF, Goll MG, Bagnat M
Proceedings of the National Academy of Sciences of the United States of America112(9)2770-5(2015 Mar)

DNA hypomethylation induces a DNA replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish embryos.
Jacob V, Chernyavskaya Y, Chen X, Tan PS, Kent B, Hoshida Y, Sadler KC
Development (Cambridge, England)142(3)510-21(2015 Feb)

Parp inhibition prevents ten-eleven translocase enzyme activation and hyperglycemia-induced DNA demethylation.
Dhliwayo N, Sarras MP Jr, Luczkowski E, Mason SM, Intine RV
Diabetes63(9)3069-76(2014 Sep)

UHRF1 overexpression drives DNA hypomethylation and hepatocellular carcinoma.
Mudbhary R, Hoshida Y, Chernyavskaya Y, Jacob V, Villanueva A, Fiel MI, Chen X, Kojima K, Thung S, Bronson RT, Lachenmayer A, Revill K, Alsinet C, Sachidanandam R, Desai A, SenBanerjee S, Ukomadu C, Llovet JM, Sadler KC
Cancer cell25(2)196-209(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dnmt1 stability requires UHRF1 phosphorylation and that crosstalk between the proteins is essential for the function of these two important epigenetic regulators during gastrulation
Title: UHRF1 regulation of Dnmt1 is required for pre-gastrula zebrafish development.

DNA hypomethylation is the mechanism underlying the cell cycle block and small liver phenotype caused by uhrf1 depletion.
Title: DNA hypomethylation induces a DNA replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish embryos.

an essential role for UHRF1 phosphorylation by cyclin-dependent kinase 2/cyclin A2 during early vertebrate development
Title: UHRF1 phosphorylation by cyclin A2/cyclin-dependent kinase 2 is required for zebrafish embryogenesis.

These data provide the first evidence that Uhrf1 and Dnmt1 function is required for vertebrate lens development and maintenance.
Title: Uhrf1 and Dnmt1 are required for development and maintenance of the zebrafish lens.

uhrf1 is required for physiologic liver growth in both embryos and adults
Title: Liver growth in the embryo and during liver regeneration in zebrafish requires the cell cycle regulator, uhrf1.

The following uhrf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the uhrf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03458	NM_213077.1 Latest version!	Danio rerio ubiquitin-like with PHD and ring finger domains 1 (uhrf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

uhrf1 ( NM_213077.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998242.1 Danio rerio ubiquitin-like with PHD and ring finger domains 1 (uhrf1), mRNA.

Price & Availability↑

CloneID	ODa03458
Clone ID Related Accession (Same CDS sequence)	NM_213077.1
Accession Version	NM_213077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UHRF1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC058055.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058055.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGGATTC AGGTGCGCAC TATGGATGGT AAGGAGACCC ATCGGGTCGA CTCCCTGTCC 
AAGCTCACCA AGGTGGACGA GCTGCGTGTG AAGATCGCCG AACTCTTCAA CGTCGAACCC 
GAGCGGCAGA GACTCTTTTA CAGGGGCAAA CAGATGGAGG ATGGTCACAC CATATTTGAC 
TATAACGTGG GTCTAAACGA CATCGTTCAG CTGTTGGTTC GGCAGGCGGT TGCAGCCACG 
GTGCTACCAA AAGACAAGGA GGCAGAGCTG TCGGACTCCG ACTCCGGCTG CGGATCGGCT 
CAGAGCGAGT CTGATAAAGG ATCCACACAT GGTGAGAGCG ACGTACAGAG CGCCGGAGCC 
TCTGGACAGA CAGACACAGC AGACCTCATC GACCCTGGCT TTGGCTTTTA CAAGATAAAT 
GAGTTTGTGG ATGCTCGAGA CCTGAATATG GGTGCCTGGT TCGAGGCTCA GATCGTCAAA 
GTGACGAAAA CTCCAGCTGA AGATGGAGGA CCTGAGGAGA TCGTTTACCA CGTCAAATAT 
GAAGATTACC CAGAGAACGG TGTGGTGCAG CTGCGTGGGA AAGACGTCCG TCCGCGGGCG 
CGAACCGTGT ACCAGTGGCG CCAGCTGGAG CCGGGAATGA TTGTGATGGT GAACTACAAC 
CCTGACGACC CCAAGGAGCG CGGATACTGG TACGACGCCG AGATCCAGAG GAAGAGGGAG 
ACGCGCACAC AGCGGGAGGT GTTCGGCAAG ATCCTGCTCG GGGATGCTGG TGATTCCCTG 
AACGACTGTC GCATCATGTT TGTGACTGAA ATCTACAAGA TCGAGGAGCC GGGAAGTGCC 
GAGGGTCCAG GAGCCTCTTC TGACAGCCCT CTCAAGAGGT CGAACGGTCC GGAGTGTAAG 
GTGTGCAAAG ACGACCCGAA GAAGAACTGC CGCGTCTGCA ACTGTCACGT GTGCGGCATA 
AAGCAGGACC CCGACAAACA GCTGCTGTGT GACGAGTGCG ACATGGCCTT TCACACCTAC 
TGCCTGAACC CACCGCTCAC CACCATACCT GACGACGAAG ACTGGTATTG TCCCGATTGT 
CGTAATGACG CCAGTGAGGT GGTTCTGGCC GGAGAGAAGC TAAAGGAGAG CAAGAAGAAA 
GCCAAGATGG CGTCTGCCAG CTCGTCCAGC CAGAGGGACT GGGGGAAGGG TATGGCGTGT 
GTGGGACGCA CCAAACAGTG CACCATCGTC CCCTCAAACC ACTACGGCCC TGTTCCTGGA 
GTCCCGGTGG GAACACTCTG GAAATTCAGG GTACAGGTCA GTGAATCAGG CGTCCACAGG 
CCTCACGTGG CAGGAATTCA CGGCAGGAGC AACGACGGCG CTTATTCTCT GGTTCTGGCT 
GGAGGTTATG AAGACGACGT GGATGATGGG AATGAGTTCA CCTACACAGG CTCCGGTGGA 
CGAGACCTGT CCGGAAACAA AAGGACGGCC GAGCAGTCCT GCGACCAGAA GCTGACCAAC 
ATGAACCGGG CTCTCGCTCT GAACTGTAAC GCAGCAGTGA ATGATAAGGA AGGAGCTGAA 
GCTAAAGACT GGAAGGCTGG GAAACCGGTG CGAGTCGTGC GCAGCTCTAA AGGACGCAAA 
CACAGCAAAT ACTCTCCCGA AGACGGAAAC CGATACGACG GCATCTACAA GGTGGTGAAG 
TACTGGCCGG AGAAGGGCAA GTCTGGATTC CTGGTCTGGA GGTATTTGCT GAAGCGAAAC 
GATGAGGAAT CCGCACCGTG GACCCGAGAT GGAAAAGAGC GCATCAAGAA ACTGGGCCTC 
ACCATGCAGT ATCCTGAAGG CTATCTGGAG GCTGTAGCTG CAAAAGAGAA GGAAAAAGAG 
AACAAAAACG AAGACGACAT TGAAGAAACG CCAACCAAAG GCAAGAGGAA GAGGAAATCC 
CAGAGCATGG AGGAGAAGAG CTCTCCGACC AAGGGGACGC CGAAGAAGAT GAAGGTGGAG 
GCGTACAAGC TGAGCAAAGA GCAGAAGGCG CTGATCAAAG ACGACGAGCT CAACAAGAAG 
CTGTGGGACG AGGCCATGGA GTCGCTGAGT CTCGGGCCGC GCTTTGTTAA TAAAGTGGAG 
GAGGTTTTCC TGTGCATCTG CTGCCAGGAG GTCGTCTATC AGCCCATTAC TACAGAGTGC 
CAACACAACG TCTGCAGGGA ATGCCTTCAG AGATCTTTCA AAGCCGAGGT CTACACCTGT 
CCGGCCTGCC GACACGACCT GGGCAAAAAC TACCAAGTGG CGGTCAACAA ACCCCTCCAA 
GCCATCCTCA CCCAGCTCTT CCCCGGATAC AGCAGTGGCC GGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998242.1
CDS	113..2440
Translation	MWIQVRTMDGKETHRVDSLSKLTKVDELRVKIAELFNVEPERQRLFYRGKQMEDGHTIFDYNVGLNDIVQLLVRQAVAATVLPKDKEAELSDSDSGCGSAQSESDKGSTHGESDVQSAGASGQTDTADLIDPGFGFYKINEFVDARDLNMGAWFEAQIVKVTKTPAEDGGPEEIVYHVKYEDYPENGVVQLRGKDVRPRARTVYQWRQLEPGMIVMVNYNPDDPKERGYWYDAEIQRKRETRTQREVFGKILLGDAGDSLNDCRIMFVTEIYKIEEPGSAEGPGASSDSPLKRSNGPECKVCKDDPKKNCRVCNCHVCGIKQDPDKQLLCDECDMAFHTYCLNPPLTTIPDDEDWYCPDCRNDASEVVLAGEKLKESKKKAKMASASSSSQRDWGKGMACVGRTKQCTIVPSNHYGPVPGVPVGTLWKFRVQVSESGVHRPHVAGIHGRSNDGAYSLVLAGGYEDDVDDGNEFTYTGSGGRDLSGNKRTAEQSCDQKLTNMNRALALNCNAAVNDKEGAEAKDWKAGKPVRVVRSSKGRKHSKYSPEDGNRYDGIYKVVKYWPEKGKSGFLVWRYLLKRNDEESAPWTRDGKERIKKLGLTMQYPEGYLEAVAAKEKEKENKNEDDIEETPTKGKRKRKSQSMEEKSSPTKGTPKKMKVEAYKLSKEQKALIKDDELNKKLWDEAMESLSLGPRFVNKVEEVFLCICCQEVVYQPITTECQHNVCRECLQRSFKAEVYTCPACRHDLGKNYQVAVNKPLQAILTQLFPGYSSGRC

Target ORF information:

RefSeq Version	NM_213077.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin-like with PHD and ring finger domains 1 (uhrf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213077.1

ORF Insert Sequence:

ATGTGGATTC AGGTGCGCAC TATGGATGGT AAGGAGACCC ATCGGGTCGA CTCCCTGTCC 
AAGCTCACCA AGGTGGACGA GCTGCGTGTG AAGATCGCCG AACTCTTCAA CGTCGAACCC 
GAGCGGCAGA GACTCTTTTA CAGGGGCAAA CAGATGGAGG ATGGTCACAC CATATTTGAC 
TATAACGTGG GTCTAAACGA CATCGTTCAG CTGTTGGTTC GGCAGGCGGT TGCAGCCACG 
GTGCTACCAA AAGACAAGGA GGCAGAGCTG TCGGACTCCG ACTCCGGCTG CGGATCGGCT 
CAGAGCGAGT CTGATAAAGG ATCCACACAT GGTGAGAGCG ACGTACAGAG CGCCGGAGCC 
TCTGGACAGA CAGACACAGC AGACCTCATC GACCCTGGCT TTGGCTTTTA CAAGATAAAT 
GAGTTTGTGG ATGCTCGAGA CCTGAATATG GGTGCCTGGT TCGAGGCTCA GATCGTCAAA 
GTGACGAAAA CTCCAGCTGA AGATGGAGGA CCTGAGGAGA TCGTTTACCA CGTCAAATAT 
GAAGATTACC CAGAGAACGG TGTGGTGCAG CTGCGTGGGA AAGACGTCCG TCCGCGGGCG 
CGAACCGTGT ACCAGTGGCG CCAGCTGGAG CCGGGAATGA TTGTGATGGT GAACTACAAC 
CCTGACGACC CCAAGGAGCG CGGATACTGG TACGACGCCG AGATCCAGAG GAAGAGGGAG 
ACGCGCACAC AGCGGGAGGT GTTCGGCAAG ATCCTGCTCG GGGATGCTGG TGATTCCCTG 
AACGACTGTC GCATCATGTT TGTGACTGAA ATCTACAAGA TCGAGGAGCC GGGAAGTGCC 
GAGGGTCCAG GAGCCTCTTC TGACAGCCCT CTCAAGAGGT CGAACGGTCC GGAGTGTAAG 
GTGTGCAAAG ACGACCCGAA GAAGAACTGC CGCGTCTGCA ACTGTCACGT GTGCGGCATA 
AAGCAGGACC CCGACAAACA GCTGCTGTGT GACGAGTGCG ACATGGCCTT TCACACCTAC 
TGCCTGAACC CACCGCTCAC CACCATACCT GACGACGAAG ACTGGTATTG TCCCGATTGT 
CGTAATGACG CCAGTGAGGT GGTTCTGGCC GGAGAGAAGC TAAAGGAGAG CAAGAAGAAA 
GCCAAGATGG CGTCTGCCAG CTCGTCCAGC CAGAGGGACT GGGGGAAGGG TATGGCGTGT 
GTGGGACGCA CCAAACAGTG CACCATCGTC CCCTCAAACC ACTACGGCCC TGTTCCTGGA 
GTCCCGGTGG GAACACTCTG GAAATTCAGG GTACAGGTCA GTGAATCAGG CGTCCACAGG 
CCTCACGTGG CAGGAATTCA CGGCAGGAGC AACGACGGCG CTTATTCTCT GGTTCTGGCT 
GGAGGTTATG AAGACGACGT GGATGATGGG AATGAGTTCA CCTACACAGG CTCCGGTGGA 
CGAGACCTGT CCGGAAACAA AAGGACGGCC GAGCAGTCCT GCGACCAGAA GCTGACCAAC 
ATGAACCGGG CTCTCGCTCT GAACTGTAAC GCAGCAGTGA ATGATAAGGA AGGAGCTGAA 
GCTAAAGACT GGAAGGCTGG GAAACCGGTG CGAGTCGTGC GCAGCTCTAA AGGACGCAAA 
CACAGCAAAT ACTCTCCCGA AGACGGAAAC CGATACGACG GCATCTACAA GGTGGTGAAG 
TACTGGCCGG AGAAGGGCAA GTCTGGATTC CTGGTCTGGA GGTATTTGCT GAAGCGAAAC 
GATGAGGAAT CCGCACCGTG GACCCGAGAT GGAAAAGAGC GCATCAAGAA ACTGGGCCTC 
ACCATGCAGT ATCCTGAAGG CTATCTGGAG GCTGTAGCTG CAAAAGAGAA GGAAAAAGAG 
AACAAAAACG AAGACGACAT TGAAGAAACG CCAACCAAAG GCAAGAGGAA GAGGAAATCC 
CAGAGCATGG AGGAGAAGAG CTCTCCGACC AAGGGGACGC CGAAGAAGAT GAAGGTGGAG 
GCGTACAAGC TGAGCAAAGA GCAGAAGGCG CTGATCAAAG ACGACGAGCT CAACAAGAAG 
CTGTGGGACG AGGCCATGGA GTCGCTGAGT CTCGGGCCGC GCTTTGTTAA TAAAGTGGAG 
GAGGTTTTCC TGTGCATCTG CTGCCAGGAG GTCGTCTATC AGCCCATTAC TACAGAGTGC 
CAACACAACG TCTGCAGGGA ATGCCTTCAG AGATCTTTCA AAGCCGAGGT CTACACCTGT 
CCGGCCTGCC GACACGACCT GGGCAAAAAC TACCAAGTGG CGGTCAACAA ACCCCTCCAA 
GCCATCCTCA CCCAGCTCTT CCCCGGATAC AGCAGTGGCC GGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

UHRF1 regulation of Dnmt1 is required for pre-gastrula zebrafish development.
Developmental biology412(1)99-113(2016 Apr)
Kent B,Magnani E,Walsh MJ,Sadler KC

Epigenetic control of intestinal barrier function and inflammation in zebrafish.
Proceedings of the National Academy of Sciences of the United States of America112(9)2770-5(2015 Mar)
Marjoram L,Alvers A,Deerhake ME,Bagwell J,Mankiewicz J,Cocchiaro JL,Beerman RW,Willer J,Sumigray KD,Katsanis N,Tobin DM,Rawls JF,Goll MG,Bagnat M

DNA hypomethylation induces a DNA replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish embryos.
Development (Cambridge, England)142(3)510-21(2015 Feb)
Jacob V,Chernyavskaya Y,Chen X,Tan PS,Kent B,Hoshida Y,Sadler KC

Parp inhibition prevents ten-eleven translocase enzyme activation and hyperglycemia-induced DNA demethylation.
Diabetes63(9)3069-76(2014 Sep)
Dhliwayo N,Sarras MP Jr,Luczkowski E,Mason SM,Intine RV

UHRF1 overexpression drives DNA hypomethylation and hepatocellular carcinoma.
Cancer cell25(2)196-209(2014 Feb)
Mudbhary R,Hoshida Y,Chernyavskaya Y,Jacob V,Villanueva A,Fiel MI,Chen X,Kojima K,Thung S,Bronson RT,Lachenmayer A,Revill K,Alsinet C,Sachidanandam R,Desai A,SenBanerjee S,Ukomadu C,Llovet JM,Sadler KC

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uhrf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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