shroom2a gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_017356637.2	XP_017212126.1	protein Shroom2 isoform X5
XM_017356639.1	XP_017212128.1	protein Shroom2 isoform X7
XM_017356635.1	XP_017212124.1	protein Shroom2 isoform X4
XM_017356637.1	XP_017212126.1	protein Shroom2 isoform X6
XM_017356638.1	XP_017212127.1	protein Shroom2 isoform X6
XM_017356634.2	XP_017212123.1	protein Shroom2 isoform X3
XM_017356639.2	XP_017212128.1	protein Shroom2 isoform X7
XM_017356633.1	XP_017212122.1	protein Shroom2 isoform X2
XM_017356636.1	XP_017212125.1	protein Shroom2 isoform X5
XM_017356636.2	XP_017212125.1	protein Shroom2 isoform X4
XM_017356638.2	XP_017212127.1	protein Shroom2 isoform X5
XM_017356634.1	XP_017212123.1	protein Shroom2 isoform X3
XM_017356632.2	XP_017212121.1	protein Shroom2 isoform X1
XM_021477154.1	XP_021332829.1	protein Shroom2 isoform X5
XM_017356632.1	XP_017212121.1	protein Shroom2 isoform X1
XM_017356633.2	XP_017212122.1	protein Shroom2 isoform X2
XM_021477152.1	XP_021332827.1	protein Shroom2 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa143082	XM_021477152.1 Latest version!	Danio rerio shroom family member 2a (shroom2a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa59471	XM_017356632.2 Latest version!	Danio rerio shroom family member 2a (shroom2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa59472	XM_017356633.2 Latest version!	Danio rerio shroom family member 2a (shroom2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa59473	XM_017356634.2 Latest version!	Danio rerio shroom family member 2a (shroom2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa59474	XM_017356635.1 Latest version!	Danio rerio shroom family member 2a (shroom2a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa59475	XM_017356636.2 Latest version!	Danio rerio shroom family member 2a (shroom2a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa59476	XM_017356638.2 Latest version!	Danio rerio shroom family member 2a (shroom2a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa59476	XM_017356637.2 Latest version!	Danio rerio shroom family member 2a (shroom2a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa59476	XM_021477154.1 Latest version!	Danio rerio shroom family member 2a (shroom2a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa59477	XM_017356639.2 Latest version!	Danio rerio shroom family member 2a (shroom2a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
ODa59476	XM_017356637.1	Danio rerio shroom family member 2a (shroom2a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa59477	XM_017356639.1	Danio rerio shroom family member 2a (shroom2a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa59475	XM_017356636.1	Danio rerio shroom family member 2a (shroom2a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa59476	XM_017356638.1	Danio rerio shroom family member 2a (shroom2a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa59473	XM_017356634.1	Danio rerio shroom family member 2a (shroom2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa59471	XM_017356632.1	Danio rerio shroom family member 2a (shroom2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa59472	XM_017356633.1	Danio rerio shroom family member 2a (shroom2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

shroom2a ( XM_021477152.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332827.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa143082
Clone ID Related Accession (Same CDS sequence)	XM_021477152.1
Accession Version	XM_021477152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGG TGGATATTGT AGCGCAGAAA ATGCCATCAG AGAGCGACGT GCATATGGCC 
CGGAGCTTTC TCACGAAGAT TTTACGAAGT TCCATGAGGA GAAATGAACC CATGAGCAGA 
CCTCACTCCT GGCATGCCAC CAAGTTCAAC GAGACCCATT CAGAAGCCAA AACCCAGTCC 
ACACCCTCAC CAGTTTGGCA AACAAGATAT GATGCAAGTT CATTCTCTTC TGAACTCTCA 
ACTGGCTGGG ATCAAACAAA TCTACGGAGA GTATCCGATC AGTTCAGTTC TTTGGGGAGT 
ATGGACAGCC TAGAACATAG TTCTCATCCC TATCCACCTG GCCGTCTTTC CCCAAACAAA 
TCCAACAATA ATAGCATTGA ACATTTGGGT AGTGGTAAAC GGGACTCGGC ATACAGCTCT 
TTTTCTACAA GTTCGGGAAC CCCAGATTAC ACACTGTCCA AAAGTAACAC GGCATCCACT 
GAGAACATGC TGTACAAAAT AAATCAGTGG GATTCAAGTG CCAGGCACAG CAATGGCAGG 
CACAGCCAAA ACCTAAGCGA AGGGGTACGA CAAGATGAGA GGCCTGGATA CCTGCACCAC 
CTCTCAAGTA CAGGTAACCA CGAGATCCCA AGAGCTGAAG AACTGCCTGG CAACCGTCAT 
TCAAGCTCTG GAAGAGTTAA TATTGGACCT GTCTGGCATG TTCCAGATAT GAAGAAAAAC 
ATGATATCAT CTACTCCACC TCCCACTCCA CCCACACGCA GTGATAGCTT TGCAGCTACC 
AAGGTTCACG AGAAGGGTCT GATCACAGGT ACTTCAGAGG GCCTTGGTGG TCATTCTCAA 
GTAAAGCCTC AAGCGAAACC ACAACAAAAG CCAGGAGAAA CCCATGAAAG CACACAAAGG 
TCTAACCAAG TCATCGAGAT TTGTCTTGAG GGTCGACGGG ATTATAATCT ACAATCCAAA 
AATGATTCCT CAAATAGCTG CATTTCATCT GATTCTCATC ATCAGTATAT CCAACGTACA 
TCATCAGATA AGACATACTC CCTATCAACC ACAGACATAA GAGATAGCCA TCCATCCTAT 
GGCCATGCTC CATACCATGC ACAGCAGTAC AGTGACGAAG GCACTTTCCA TGCTCAAACT 
AGAACAGCAA TGAAACCTCC ATTCAGTGGC TATTTCAGTA GTATGCAGGA ACTGCACACA 
AACAACCACA TGCAACTCAA CAGCCAGAAT CAAACTGGAA GACCAGCTGC ATCACTGTCA 
GTTAATTTAG GAGTCAATTC CCACCATATC TCTCAGGGAT TAACACAGGC TTCTTTAATG 
AGAGCTGATG ACTCTAAAGC CTCTTCAGTC TCAGAAATGT CCCACAATGG ACGGGACAGA 
ATGTCCATAG GTTCTCAAGG AGGGAATAAA GAGCGGTATT TCCCTCCTCA ATCGCAGCAC 
CATGATAATA AAGAAAGAAG TGCCTCCTTT AAACAAAATG AAAACCAACA TTATGTTTCT 
GCACCAAGTA ATCCCTTGAA TATGTCTGAT TCAACAAAAC ATAATGAGCT TAGGGGATGC 
CAAAAGCAGC ACCATGTGCA TGGCTCAGAT GAGCTTTCTG GTAGTTATCC ACCCAGTAAG 
CAGCCCGAGC ACAGGAGAAG TGCTGGCCAT CTTCATCATA AGGAATACTC GCAACAACCC 
TTTCCAACTA AAAGCGAATC AAAGATATGT CCCCAGAAAA CACCTATGTT GTATTCTCTG 
GCCCAAGGGC ACAATGACAC AGATGACTGT TCTAATCAGA TCAAAAGTGG AAGCGCACAA 
CATGAAGCCC TTGACAGTCT CAGTAGTAAA CAGGTTAGAC GGAATGACCG ATTTGCCACC 
ACTTTGCGCA ACGAGATCCA GATGAGAAGG GCGCATCTCC AAAAGAGTCA AAGTGCAGCC 
ACTTTAGAAA GTCCAGTTGA AACTGTAGAA GAGTCTGCTT TGTGGAAGAC CACAGGGACT 
GCTTCTCCCT CATCAGATGG CTGCTTTTCC ATTTCTTATA AAGACCATCT TAAAGAAGCT 
CAGGCCAGAG TCCTCCAGGC TACATCATTC AGGAGAAAAG ACCTAGAGCC AGTATTATTT 
GAGCATCCAG GTACTGAAGG TCCCACACGA AAAGATGTAC CTCCTTCAAG TGTCTCAGAG 
GTCACACCTA GCAAACCCAC CTCAGGGAGT AATCATGTGC TTCGCATTGG AGGCCGTAAG 
CGGATATCTG CTGAAAAGAA AGTGACGTCT TTTTCTGAGC CAGATAAAAT TCATGAAGTA 
GGAGCTGATG AACGCTCCAC TATGCCTGAA AATGTTGCAC ACTTAGAAAA TCAACAAAGA 
TTCTTTGAGA CAACAGGGAA ACCAGTTTTC CCTAAGCCTA TGCCAAAGCA AAACCTGCAG 
ATATCAGAAG ACCCCAGACT GTCCAAGCCT GGAGGCATGC ACTATTCTGC AAACAGTGAT 
ATGGCAGGGC GGAATAATGG TGAAAGCTCA ACCCCCATTG AACACCACAT TAATGAAGGT 
CTGGATGGCC CTAACTCAGT GAACCATCAA GCATTGCTAG AACAACAGCG ACTAGGCACA 
TTTGCAGAGT ATGAGGCCAA ATGGAACACC CAGAGGAAGA CTACAGAACC AAGAGTATCT 
GGCCGATACC ACTCTGCTGA TAACATCCTC GATACAGGAA AAGAGCGACG GAGTAACCCC 
ACCTGTGTGC ATGAAAGATC TAGATCCTCA CCTTCTGCTG ACTTTTATGG GCAGAAACTT 
CCACCACAAG AAAAGAAGTC AGCTGACTGT TTCAAATCTG AAAAGAGTCC TTGCCAACAG 
GACAAGAATA GTACAAGTCA AAATTTCTAC CCATCTGTTT CTTTGGAGCA CAGCCACTGG 
TTCTATACAC CCAGCAGGTT AAATGAAAAA GGATATAGTG AAATCTTATG CAAGGAGAAA 
CCAGAAACAC CTCCTGAAGA ACCTGAGAAA AAGACTGTAG ATCCTCCCTC CAGCCATCAT 
AAGCCTGCAC TACATTTCCC TGTCCATAGC ACTCGCAGTG AACCATCAGC CCTCTCCAAA 
AACAAGAGCT CATTTCTCTT GCCCCATCTG GAGAAGTACA AATGCCCTGA GGGAACACCT 
CCTCCTCTTA GCAATATTCA GGAGGTCCAG CCTGGATCAC AAGGAGGAGC TCTGGCCCCA 
ATTTCCCCAG TCAACAATCA AAGCTCCGCC CCAGTTTCAG CCCCCATTCC TTGGAAGAGT 
CCAGAGTACA GCAAAGGGCC TGTCGGGGAG GAGGAGCAAC ACCAAGAGCT TGTGCCTCCT 
CCCTTTCCAC CTCCTCCCCC TCCCGTTGTC CTGCCCACCC AACAAACCGC TGGCCCGACC 
CAGCCCACAA TGGATGGGCA GCGTTCGCCC TCGCCCCAGT TCGCTCCCCA GAGGCTGACT 
GACAAGCCCC CTGTCTCCGT CTCCTCACAG GATGAAGCTC CTGGAAGGAT GGATCAGATT 
AAAGATGAGA ACACATCTGT GAAGAAAGTG CCCATTAAGA TAGTCCATTC AGAGAGTGAC 
ACGGAAAAAG AAAGTCGGCA ATATCTAGAC CTGCCCAGCG AGACGCCTGT CAACTCGCAG 
GGACTTGTGG GAGCTCATCT TCAAAGTTTG GGGAACCCGG ATCAGTCATA CTCTTTGTTT 
TGTACATACA CCAGACAAAA AGATCAGGTT CCTGGTCTTA GAGACCCAGA CATGGGCCCT 
CTAAAAGACC AGGGGCCACA AAGCAATATA AACCCAGTGT CTTATGTGGA ACCTGGCCTT 
CAGACAGGTC CACCATTGGA TCAGAGTACC AATGGAGTGT CTTTGCATCC TTCACATTCA 
GAGGATGATA AAAGAAAGGA GCTGGCTAGA GACATAATGG ACAAAGACAA GTCCCTCGCT 
GACATTTTAG ACCAGAGTAA GATGAAGACC ACTATGGATC TGATGGAGGG GATTTTCCCT 
CAAGGAGATC AGCTACTGGA GGAGGCCCAA CAGCGTAGGA AGGCTCAACC TAAACCACTC 
TCCCCTCGAA ACTCTGTTGA GAAGAAAGAG GAAGACAGCC TGGTGGCGGC CGCTGCTTTA 
GTGAGCAACT CAACCTACTA CAGCACATCT GCACCCAAAG CAGAGCTGCT GATCAAGATG 
AAGGACATGC AGGACCAGAG CGTTGAACAC AACTCAGAAG AGGAGCTGGA GGAGGACAAT 
GAAAGTGACC TCGCCTGCAA GAAGCACGAG TTGATTGAAA GTCTCAGTAA GAAGTTGCAG 
GTCTTAAAAG AAGCTCAGGA GAGCCTGCAG GAGGACGTGC AGGACAACAA CGCTCTTGGA 
GAAGAAGTGG AGGCCATCGT ACAGGGCGTC TGCAAGCCTA ATGAACTCGA CAAATTCCGC 
ATGTTCGTCG GGGATCTTGA TAAAGTGGTC AACCTTCTGC TGTCTCTGTC AGGACGTTTG 
GCCAGGGTAG AGAATGCCCT GAACAGCCTG GAGGAAGATG CTTCGCTGGA GGAAAGGCGC 
ACTCTGACAG AGAAACGCAA ACTGCTGATT CGTCAGCACG AGGATGCTAA GGAGCTGAAG 
GAGAACCTGG ACAGGAGAGA GCGTGTGGTC TACGACATTC TGGCCAGTTA CCTGCCTGAG 
GAGAGTATGG CTGACTACGA GCACTTTGTC AAGATGAAAT CCGCTCTTAT TATCGAGCAA 
CGCAAGCTGG AGGACAAGAT CAAACTGGGA GATGAGCAGC TCAAGTGTCT GATGGACAGT 
CTCCCAATGG ACCAGAGAAT AATCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332827.1
CDS	625..5394
Translation	MKMVDIVAQKMPSESDVHMARSFLTKILRSSMRRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_021477152.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477152.1

ORF Insert Sequence:

ATGAAAATGG TGGATATTGT AGCGCAGAAA ATGCCATCAG AGAGCGACGT GCATATGGCC 
CGGAGCTTTC TCACGAAGAT TTTACGAAGT TCCATGAGGA GAAATGAACC CATGAGCAGA 
CCTCACTCCT GGCATGCCAC CAAGTTCAAC GAGACCCATT CAGAAGCCAA AACCCAGTCC 
ACACCCTCAC CAGTTTGGCA AACAAGATAT GATGCAAGTT CATTCTCTTC TGAACTCTCA 
ACTGGCTGGG ATCAAACAAA TCTACGGAGA GTATCCGATC AGTTCAGTTC TTTGGGGAGT 
ATGGACAGCC TAGAACATAG TTCTCATCCC TATCCACCTG GCCGTCTTTC CCCAAACAAA 
TCCAACAATA ATAGCATTGA ACATTTGGGT AGTGGTAAAC GGGACTCGGC ATACAGCTCT 
TTTTCTACAA GTTCGGGAAC CCCAGATTAC ACACTGTCCA AAAGTAACAC GGCATCCACT 
GAGAACATGC TGTACAAAAT AAATCAGTGG GATTCAAGTG CCAGGCACAG CAATGGCAGG 
CACAGCCAAA ACCTAAGCGA AGGGGTACGA CAAGATGAGA GGCCTGGATA CCTGCACCAC 
CTCTCAAGTA CAGGTAACCA CGAGATCCCA AGAGCTGAAG AACTGCCTGG CAACCGTCAT 
TCAAGCTCTG GAAGAGTTAA TATTGGACCT GTCTGGCATG TTCCAGATAT GAAGAAAAAC 
ATGATATCAT CTACTCCACC TCCCACTCCA CCCACACGCA GTGATAGCTT TGCAGCTACC 
AAGGTTCACG AGAAGGGTCT GATCACAGGT ACTTCAGAGG GCCTTGGTGG TCATTCTCAA 
GTAAAGCCTC AAGCGAAACC ACAACAAAAG CCAGGAGAAA CCCATGAAAG CACACAAAGG 
TCTAACCAAG TCATCGAGAT TTGTCTTGAG GGTCGACGGG ATTATAATCT ACAATCCAAA 
AATGATTCCT CAAATAGCTG CATTTCATCT GATTCTCATC ATCAGTATAT CCAACGTACA 
TCATCAGATA AGACATACTC CCTATCAACC ACAGACATAA GAGATAGCCA TCCATCCTAT 
GGCCATGCTC CATACCATGC ACAGCAGTAC AGTGACGAAG GCACTTTCCA TGCTCAAACT 
AGAACAGCAA TGAAACCTCC ATTCAGTGGC TATTTCAGTA GTATGCAGGA ACTGCACACA 
AACAACCACA TGCAACTCAA CAGCCAGAAT CAAACTGGAA GACCAGCTGC ATCACTGTCA 
GTTAATTTAG GAGTCAATTC CCACCATATC TCTCAGGGAT TAACACAGGC TTCTTTAATG 
AGAGCTGATG ACTCTAAAGC CTCTTCAGTC TCAGAAATGT CCCACAATGG ACGGGACAGA 
ATGTCCATAG GTTCTCAAGG AGGGAATAAA GAGCGGTATT TCCCTCCTCA ATCGCAGCAC 
CATGATAATA AAGAAAGAAG TGCCTCCTTT AAACAAAATG AAAACCAACA TTATGTTTCT 
GCACCAAGTA ATCCCTTGAA TATGTCTGAT TCAACAAAAC ATAATGAGCT TAGGGGATGC 
CAAAAGCAGC ACCATGTGCA TGGCTCAGAT GAGCTTTCTG GTAGTTATCC ACCCAGTAAG 
CAGCCCGAGC ACAGGAGAAG TGCTGGCCAT CTTCATCATA AGGAATACTC GCAACAACCC 
TTTCCAACTA AAAGCGAATC AAAGATATGT CCCCAGAAAA CACCTATGTT GTATTCTCTG 
GCCCAAGGGC ACAATGACAC AGATGACTGT TCTAATCAGA TCAAAAGTGG AAGCGCACAA 
CATGAAGCCC TTGACAGTCT CAGTAGTAAA CAGGTTAGAC GGAATGACCG ATTTGCCACC 
ACTTTGCGCA ACGAGATCCA GATGAGAAGG GCGCATCTCC AAAAGAGTCA AAGTGCAGCC 
ACTTTAGAAA GTCCAGTTGA AACTGTAGAA GAGTCTGCTT TGTGGAAGAC CACAGGGACT 
GCTTCTCCCT CATCAGATGG CTGCTTTTCC ATTTCTTATA AAGACCATCT TAAAGAAGCT 
CAGGCCAGAG TCCTCCAGGC TACATCATTC AGGAGAAAAG ACCTAGAGCC AGTATTATTT 
GAGCATCCAG GTACTGAAGG TCCCACACGA AAAGATGTAC CTCCTTCAAG TGTCTCAGAG 
GTCACACCTA GCAAACCCAC CTCAGGGAGT AATCATGTGC TTCGCATTGG AGGCCGTAAG 
CGGATATCTG CTGAAAAGAA AGTGACGTCT TTTTCTGAGC CAGATAAAAT TCATGAAGTA 
GGAGCTGATG AACGCTCCAC TATGCCTGAA AATGTTGCAC ACTTAGAAAA TCAACAAAGA 
TTCTTTGAGA CAACAGGGAA ACCAGTTTTC CCTAAGCCTA TGCCAAAGCA AAACCTGCAG 
ATATCAGAAG ACCCCAGACT GTCCAAGCCT GGAGGCATGC ACTATTCTGC AAACAGTGAT 
ATGGCAGGGC GGAATAATGG TGAAAGCTCA ACCCCCATTG AACACCACAT TAATGAAGGT 
CTGGATGGCC CTAACTCAGT GAACCATCAA GCATTGCTAG AACAACAGCG ACTAGGCACA 
TTTGCAGAGT ATGAGGCCAA ATGGAACACC CAGAGGAAGA CTACAGAACC AAGAGTATCT 
GGCCGATACC ACTCTGCTGA TAACATCCTC GATACAGGAA AAGAGCGACG GAGTAACCCC 
ACCTGTGTGC ATGAAAGATC TAGATCCTCA CCTTCTGCTG ACTTTTATGG GCAGAAACTT 
CCACCACAAG AAAAGAAGTC AGCTGACTGT TTCAAATCTG AAAAGAGTCC TTGCCAACAG 
GACAAGAATA GTACAAGTCA AAATTTCTAC CCATCTGTTT CTTTGGAGCA CAGCCACTGG 
TTCTATACAC CCAGCAGGTT AAATGAAAAA GGATATAGTG AAATCTTATG CAAGGAGAAA 
CCAGAAACAC CTCCTGAAGA ACCTGAGAAA AAGACTGTAG ATCCTCCCTC CAGCCATCAT 
AAGCCTGCAC TACATTTCCC TGTCCATAGC ACTCGCAGTG AACCATCAGC CCTCTCCAAA 
AACAAGAGCT CATTTCTCTT GCCCCATCTG GAGAAGTACA AATGCCCTGA GGGAACACCT 
CCTCCTCTTA GCAATATTCA GGAGGTCCAG CCTGGATCAC AAGGAGGAGC TCTGGCCCCA 
ATTTCCCCAG TCAACAATCA AAGCTCCGCC CCAGTTTCAG CCCCCATTCC TTGGAAGAGT 
CCAGAGTACA GCAAAGGGCC TGTCGGGGAG GAGGAGCAAC ACCAAGAGCT TGTGCCTCCT 
CCCTTTCCAC CTCCTCCCCC TCCCGTTGTC CTGCCCACCC AACAAACCGC TGGCCCGACC 
CAGCCCACAA TGGATGGGCA GCGTTCGCCC TCGCCCCAGT TCGCTCCCCA GAGGCTGACT 
GACAAGCCCC CTGTCTCCGT CTCCTCACAG GATGAAGCTC CTGGAAGGAT GGATCAGATT 
AAAGATGAGA ACACATCTGT GAAGAAAGTG CCCATTAAGA TAGTCCATTC AGAGAGTGAC 
ACGGAAAAAG AAAGTCGGCA ATATCTAGAC CTGCCCAGCG AGACGCCTGT CAACTCGCAG 
GGACTTGTGG GAGCTCATCT TCAAAGTTTG GGGAACCCGG ATCAGTCATA CTCTTTGTTT 
TGTACATACA CCAGACAAAA AGATCAGGTT CCTGGTCTTA GAGACCCAGA CATGGGCCCT 
CTAAAAGACC AGGGGCCACA AAGCAATATA AACCCAGTGT CTTATGTGGA ACCTGGCCTT 
CAGACAGGTC CACCATTGGA TCAGAGTACC AATGGAGTGT CTTTGCATCC TTCACATTCA 
GAGGATGATA AAAGAAAGGA GCTGGCTAGA GACATAATGG ACAAAGACAA GTCCCTCGCT 
GACATTTTAG ACCAGAGTAA GATGAAGACC ACTATGGATC TGATGGAGGG GATTTTCCCT 
CAAGGAGATC AGCTACTGGA GGAGGCCCAA CAGCGTAGGA AGGCTCAACC TAAACCACTC 
TCCCCTCGAA ACTCTGTTGA GAAGAAAGAG GAAGACAGCC TGGTGGCGGC CGCTGCTTTA 
GTGAGCAACT CAACCTACTA CAGCACATCT GCACCCAAAG CAGAGCTGCT GATCAAGATG 
AAGGACATGC AGGACCAGAG CGTTGAACAC AACTCAGAAG AGGAGCTGGA GGAGGACAAT 
GAAAGTGACC TCGCCTGCAA GAAGCACGAG TTGATTGAAA GTCTCAGTAA GAAGTTGCAG 
GTCTTAAAAG AAGCTCAGGA GAGCCTGCAG GAGGACGTGC AGGACAACAA CGCTCTTGGA 
GAAGAAGTGG AGGCCATCGT ACAGGGCGTC TGCAAGCCTA ATGAACTCGA CAAATTCCGC 
ATGTTCGTCG GGGATCTTGA TAAAGTGGTC AACCTTCTGC TGTCTCTGTC AGGACGTTTG 
GCCAGGGTAG AGAATGCCCT GAACAGCCTG GAGGAAGATG CTTCGCTGGA GGAAAGGCGC 
ACTCTGACAG AGAAACGCAA ACTGCTGATT CGTCAGCACG AGGATGCTAA GGAGCTGAAG 
GAGAACCTGG ACAGGAGAGA GCGTGTGGTC TACGACATTC TGGCCAGTTA CCTGCCTGAG 
GAGAGTATGG CTGACTACGA GCACTTTGTC AAGATGAAAT CCGCTCTTAT TATCGAGCAA 
CGCAAGCTGG AGGACAAGAT CAAACTGGGA GATGAGCAGC TCAAGTGTCT GATGGACAGT 
CTCCCAATGG ACCAGAGAAT AATCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356632.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212121.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa59471
Clone ID Related Accession (Same CDS sequence)	XM_017356632.2 , XM_017356632.1
Accession Version	XM_017356632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAAAATGA AAATGGTGGA TATTGTAGCG CAGAAAATGC CATCAGAGAG CGACGTGCAT 
ATGGCCCGGA GCTTTCTCAC GAAGATTTTA CGAAGTTCCA TGAGAAAGAA TCGGTTTAAA 
GGGAGAAATG AACCCATGAG CAGACCTCAC TCCTGGCATG CCACCAAGTT CAACGAGACC 
CATTCAGAAG CCAAAACCCA GTCCACACCC TCACCAGTTT GGCAAACAAG ATATGATGCA 
AGTTCATTCT CTTCTGAACT CTCAACTGGC TGGGATCAAA CAAATCTACG GAGAGTATCC 
GATCAGTTCA GTTCTTTGGG GAGTATGGAC AGCCTAGAAC ATAGTTCTCA TCCCTATCCA 
CCTGGCCGTC TTTCCCCAAA CAAATCCAAC AATAATAGCA TTGAACATTT GGGTAGTGGT 
AAACGGGACT CGGCATACAG CTCTTTTTCT ACAAGTTCGG GAACCCCAGA TTACACACTG 
TCCAAAAGTA ACACGGCATC CACTGAGAAC ATGCTGTACA AAATAAATCA GTGGGATTCA 
AGTGCCAGGC ACAGCAATGG CAGGCACAGC CAAAACCTAA GCGAAGGGGT ACGACAAGAT 
GAGAGGCCTG GATACCTGCA CCACCTCTCA AGTACAGGTA ACCACGAGAT CCCAAGAGCT 
GAAGAACTGC CTGGCAACCG TCATTCAAGC TCTGGAAGAG TTAATATTGG ACCTGTCTGG 
CATGTTCCAG ATATGAAGAA AAACATGATA TCATCTACTC CACCTCCCAC TCCACCCACA 
CGCAGTGATA GCTTTGCAGC TACCAAGGTT CACGAGAAGG GTCTGATCAC AGGTACTTCA 
GAGGGCCTTG GTGGTCATTC TCAAGTAAAG CCTCAAGCGA AACCACAACA AAAGCCAGGA 
GAAACCCATG AAAGCACACA AAGGTCTAAC CAAGTCATCG AGATTTGTCT TGAGGGTCGA 
CGGGATTATA ATCTACAATC CAAAAATGAT TCCTCAAATA GCTGCATTTC ATCTGATTCT 
CATCATCAGT ATATCCAACG TACATCATCA GATAAGACAT ACTCCCTATC AACCACAGAC 
ATAAGAGATA GCCATCCATC CTATGGCCAT GCTCCATACC ATGCACAGCA GTACAGTGAC 
GAAGGCACTT TCCATGCTCA AACTAGAACA GCAATGAAAC CTCCATTCAG TGGCTATTTC 
AGTAGTATGC AGGAACTGCA CACAAACAAC CACATGCAAC TCAACAGCCA GAATCAAACT 
GGAAGACCAG CTGCATCACT GTCAGTTAAT TTAGGAGTCA ATTCCCACCA TATCTCTCAG 
GGATTAACAC AGGCTTCTTT AATGAGAGCT GATGACTCTA AAGCCTCTTC AGTCTCAGAA 
ATGTCCCACA ATGGACGGGA CAGAATGTCC ATAGGTTCTC AAGGAGGGAA TAAAGAGCGG 
TATTTCCCTC CTCAATCGCA GCACCATGAT AATAAAGAAA GAAGTGCCTC CTTTAAACAA 
AATGAAAACC AACATTATGT TTCTGCACCA AGTAATCCCT TGAATATGTC TGATTCAACA 
AAACATAATG AGCTTAGGGG ATGCCAAAAG CAGCACCATG TGCATGGCTC AGATGAGCTT 
TCTGGTAGTT ATCCACCCAG TAAGCAGCCC GAGCACAGGA GAAGTGCTGG CCATCTTCAT 
CATAAGGAAT ACTCGCAACA ACCCTTTCCA ACTAAAAGCG AATCAAAGAT ATGTCCCCAG 
AAAACACCTA TGTTGTATTC TCTGGCCCAA GGGCACAATG ACACAGATGA CTGTTCTAAT 
CAGATCAAAA GTGGAAGCGC ACAACATGAA GCCCTTGACA GTCTCAGTAG TAAACAGGTT 
AGACGGAATG ACCGATTTGC CACCACTTTG CGCAACGAGA TCCAGATGAG AAGGGCGCAT 
CTCCAAAAGA GTCAAAGTGC AGCCACTTTA GAAAGTCCAG TTGAAACTGT AGAAGAGTCT 
GCTTTGTGGA AGACCACAGG GACTGCTTCT CCCTCATCAG ATGGCTGCTT TTCCATTTCT 
TATAAAGACC ATCTTAAAGA AGCTCAGGCC AGAGTCCTCC AGGCTACATC ATTCAGGAGA 
AAAGACCTAG AGCCAGTATT ATTTGAGCAT CCAGGTACTG AAGGTCCCAC ACGAAAAGAT 
GTACCTCCTT CAAGTGTCTC AGAGGTCACA CCTAGCAAAC CCACCTCAGG GAGTAATCAT 
GTGCTTCGCA TTGGAGGCCG TAAGCGGATA TCTGCTGAAA AGAAAGTGAC GTCTTTTTCT 
GAGCCAGATA AAATTCATGA AGTAGGAGCT GATGAACGCT CCACTATGCC TGAAAATGTT 
GCACACTTAG AAAATCAACA AAGATTCTTT GAGACAACAG GGAAACCAGT TTTCCCTAAG 
CCTATGCCAA AGCAAAACCT GCAGATATCA GAAGACCCCA GACTGTCCAA GCCTGGAGGC 
ATGCACTATT CTGCAAACAG TGATATGGCA GGGCGGAATA ATGGTGAAAG CTCAACCCCC 
ATTGAACACC ACATTAATGA AGGTCTGGAT GGCCCTAACT CAGTGAACCA TCAAGCATTG 
CTAGAACAAC AGCGACTAGG CACATTTGCA GAGTATGAGG CCAAATGGAA CACCCAGAGG 
AAGACTACAG AACCAAGAGT ATCTGGCCGA TACCACTCTG CTGATAACAT CCTCGATACA 
GGAAAAGAGC GACGGAGTAA CCCCACCTGT GTGCATGAAA GATCTAGATC CTCACCTTCT 
GCTGACTTTT ATGGGCAGAA ACTTCCACCA CAAGAAAAGA AGTCAGCTGA CTGTTTCAAA 
TCTGAAAAGA GTCCTTGCCA ACAGGACAAG AATAGTACAA GTCAAAATTT CTACCCATCT 
GTTTCTTTGG AGCACAGCCA CTGGTTCTAT ACACCCAGCA GGTTAAATGA AAAAGGATAT 
AGTGAAATCT TATGCAAGGA GAAACCAGAA ACACCTCCTG AAGAACCTGA GAAAAAGACT 
GTAGATCCTC CCTCCAGCCA TCATAAGCCT GCACTACATT TCCCTGTCCA TAGCACTCGC 
AGTGAACCAT CAGCCCTCTC CAAAAACAAG AGCTCATTTC TCTTGCCCCA TCTGGAGAAG 
TACAAATGCC CTGAGGGAAC ACCTCCTCCT CTTAGCAATA TTCAGGAGGT CCAGCCTGGA 
TCACAAGGAG GAGCTCTGGC CCCAATTTCC CCAGTCAACA ATCAAAGCTC CGCCCCAGTT 
TCAGCCCCCA TTCCTTGGAA GAGTCCAGAG TACAGCAAAG GGCCTGTCGG GGAGGAGGAG 
CAACACCAAG AGCTTGTGCC TCCTCCCTTT CCACCTCCTC CCCCTCCCGT TGTCCTGCCC 
ACCCAACAAA CCGCTGGCCC GACCCAGCCC ACAATGGATG GGCAGCGTTC GCCCTCGCCC 
CAGTTCGCTC CCCAGAGGCT GACTGACAAG CCCCCTGTCT CCGTCTCCTC ACAGGATGAA 
GCTCCTGGAA GGATGGATCA GATTAAAGAT GAGAACACAT CTGTGAAGAA AGTGCCCATT 
AAGATAGTCC ATTCAGAGAG TGACACGGAA AAAGAAAGTC GGCAATATCT AGACCTGCCC 
AGCGAGACGC CTGTCAACTC GCAGGGACTT GTGGGAGCTC ATCTTCAAAG TTTGGGGAAC 
CCGGATCAGT CATACTCTTT GTTTTGTACA TACACCAGAC AAAAAGATCA GGTTCCTGGT 
CTTAGAGACC CAGACATGGG CCCTCTAAAA GACCAGGGGC CACAAAGCAA TATAAACCCA 
GTGTCTTATG TGGAACCTGG CCTTCAGACA GGTCCACCAT TGGATCAGAG TACCAATGGA 
GTGTCTTTGC ATCCTTCACA TTCAGAGGAT GATAAAAGAA AGGAGCTGGC TAGAGACATA 
ATGGACAAAG ACAAGTCCCT CGCTGACATT TTAGACCAGA GTAAGATGAA GACCACTATG 
GATCTGATGG AGGGGATTTT CCCTCAAGGA GATCAGCTAC TGGAGGAGGC CCAACAGCGT 
AGGAAGGCTC AACCTAAACC ACTCTCCCCT CGAAACTCTG TTGAGAAGAA AGAGGAAGAC 
AGCCTGGTGG CGGCCGCTGC TTTAGTGAGC AACTCAACCT ACTACAGCAC ATCTGCACCC 
AAAGCAGAGC TGCTGATCAA GATGAAGGAC ATGCAGGACC AGAGCGTTGA ACACAACTCA 
GAAGAGGAGC TGGAGGAGGA CAATGAAAGT GACCTCGCCT GCAAGAAGCA CGAGTTGATT 
GAAAGTCTCA GTAAGAAGTT GCAGGTCTTA AAAGAAGCTC AGGAGAGCCT GCAGGAGGAC 
GTGCAGGACA ACAACGCTCT TGGAGAAGAA GTGGAGGCCA TCGTACAGGG CGTCTGCAAG 
CCTAATGAAC TCGACAAATT CCGCATGTTC GTCGGGGATC TTGATAAAGT GGTCAACCTT 
CTGCTGTCTC TGTCAGGACG TTTGGCCAGG GTAGAGAATG CCCTGAACAG CCTGGAGGAA 
GATGCTTCGC TGGAGGAAAG GCGCACTCTG ACAGAGAAAC GCAAACTGCT GATTCGTCAG 
CACGAGGATG CTAAGGAGCT GAAGGAGAAC CTGGACAGGA GAGAGCGTGT GGTCTACGAC 
ATTCTGGCCA GTTACCTGCC TGAGGAGAGT ATGGCTGACT ACGAGCACTT TGTCAAGATG 
AAATCCGCTC TTATTATCGA GCAACGCAAG CTGGAGGACA AGATCAAACT GGGAGATGAG 
CAGCTCAAGT GTCTGATGGA CAGTCTCCCA ATGGACCAGA GAATAATCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212121.1
CDS	772..5925
Translation	MDTINQYPVNPSFPEDDHRSFCSSIRSASFNEHHVNDEGWKVVEVTLSGGAPWGFTLRGGLEHGEPLLITKVEEGSKAATVRLQVGDELININNISLTGFRQEAICLVKSSYKTLSLVVKRKMKMVDIVAQKMPSESDVHMARSFLTKILRSSMRKNRFKGRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356632.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356632.2

ORF Insert Sequence:

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAAAATGA AAATGGTGGA TATTGTAGCG CAGAAAATGC CATCAGAGAG CGACGTGCAT 
ATGGCCCGGA GCTTTCTCAC GAAGATTTTA CGAAGTTCCA TGAGAAAGAA TCGGTTTAAA 
GGGAGAAATG AACCCATGAG CAGACCTCAC TCCTGGCATG CCACCAAGTT CAACGAGACC 
CATTCAGAAG CCAAAACCCA GTCCACACCC TCACCAGTTT GGCAAACAAG ATATGATGCA 
AGTTCATTCT CTTCTGAACT CTCAACTGGC TGGGATCAAA CAAATCTACG GAGAGTATCC 
GATCAGTTCA GTTCTTTGGG GAGTATGGAC AGCCTAGAAC ATAGTTCTCA TCCCTATCCA 
CCTGGCCGTC TTTCCCCAAA CAAATCCAAC AATAATAGCA TTGAACATTT GGGTAGTGGT 
AAACGGGACT CGGCATACAG CTCTTTTTCT ACAAGTTCGG GAACCCCAGA TTACACACTG 
TCCAAAAGTA ACACGGCATC CACTGAGAAC ATGCTGTACA AAATAAATCA GTGGGATTCA 
AGTGCCAGGC ACAGCAATGG CAGGCACAGC CAAAACCTAA GCGAAGGGGT ACGACAAGAT 
GAGAGGCCTG GATACCTGCA CCACCTCTCA AGTACAGGTA ACCACGAGAT CCCAAGAGCT 
GAAGAACTGC CTGGCAACCG TCATTCAAGC TCTGGAAGAG TTAATATTGG ACCTGTCTGG 
CATGTTCCAG ATATGAAGAA AAACATGATA TCATCTACTC CACCTCCCAC TCCACCCACA 
CGCAGTGATA GCTTTGCAGC TACCAAGGTT CACGAGAAGG GTCTGATCAC AGGTACTTCA 
GAGGGCCTTG GTGGTCATTC TCAAGTAAAG CCTCAAGCGA AACCACAACA AAAGCCAGGA 
GAAACCCATG AAAGCACACA AAGGTCTAAC CAAGTCATCG AGATTTGTCT TGAGGGTCGA 
CGGGATTATA ATCTACAATC CAAAAATGAT TCCTCAAATA GCTGCATTTC ATCTGATTCT 
CATCATCAGT ATATCCAACG TACATCATCA GATAAGACAT ACTCCCTATC AACCACAGAC 
ATAAGAGATA GCCATCCATC CTATGGCCAT GCTCCATACC ATGCACAGCA GTACAGTGAC 
GAAGGCACTT TCCATGCTCA AACTAGAACA GCAATGAAAC CTCCATTCAG TGGCTATTTC 
AGTAGTATGC AGGAACTGCA CACAAACAAC CACATGCAAC TCAACAGCCA GAATCAAACT 
GGAAGACCAG CTGCATCACT GTCAGTTAAT TTAGGAGTCA ATTCCCACCA TATCTCTCAG 
GGATTAACAC AGGCTTCTTT AATGAGAGCT GATGACTCTA AAGCCTCTTC AGTCTCAGAA 
ATGTCCCACA ATGGACGGGA CAGAATGTCC ATAGGTTCTC AAGGAGGGAA TAAAGAGCGG 
TATTTCCCTC CTCAATCGCA GCACCATGAT AATAAAGAAA GAAGTGCCTC CTTTAAACAA 
AATGAAAACC AACATTATGT TTCTGCACCA AGTAATCCCT TGAATATGTC TGATTCAACA 
AAACATAATG AGCTTAGGGG ATGCCAAAAG CAGCACCATG TGCATGGCTC AGATGAGCTT 
TCTGGTAGTT ATCCACCCAG TAAGCAGCCC GAGCACAGGA GAAGTGCTGG CCATCTTCAT 
CATAAGGAAT ACTCGCAACA ACCCTTTCCA ACTAAAAGCG AATCAAAGAT ATGTCCCCAG 
AAAACACCTA TGTTGTATTC TCTGGCCCAA GGGCACAATG ACACAGATGA CTGTTCTAAT 
CAGATCAAAA GTGGAAGCGC ACAACATGAA GCCCTTGACA GTCTCAGTAG TAAACAGGTT 
AGACGGAATG ACCGATTTGC CACCACTTTG CGCAACGAGA TCCAGATGAG AAGGGCGCAT 
CTCCAAAAGA GTCAAAGTGC AGCCACTTTA GAAAGTCCAG TTGAAACTGT AGAAGAGTCT 
GCTTTGTGGA AGACCACAGG GACTGCTTCT CCCTCATCAG ATGGCTGCTT TTCCATTTCT 
TATAAAGACC ATCTTAAAGA AGCTCAGGCC AGAGTCCTCC AGGCTACATC ATTCAGGAGA 
AAAGACCTAG AGCCAGTATT ATTTGAGCAT CCAGGTACTG AAGGTCCCAC ACGAAAAGAT 
GTACCTCCTT CAAGTGTCTC AGAGGTCACA CCTAGCAAAC CCACCTCAGG GAGTAATCAT 
GTGCTTCGCA TTGGAGGCCG TAAGCGGATA TCTGCTGAAA AGAAAGTGAC GTCTTTTTCT 
GAGCCAGATA AAATTCATGA AGTAGGAGCT GATGAACGCT CCACTATGCC TGAAAATGTT 
GCACACTTAG AAAATCAACA AAGATTCTTT GAGACAACAG GGAAACCAGT TTTCCCTAAG 
CCTATGCCAA AGCAAAACCT GCAGATATCA GAAGACCCCA GACTGTCCAA GCCTGGAGGC 
ATGCACTATT CTGCAAACAG TGATATGGCA GGGCGGAATA ATGGTGAAAG CTCAACCCCC 
ATTGAACACC ACATTAATGA AGGTCTGGAT GGCCCTAACT CAGTGAACCA TCAAGCATTG 
CTAGAACAAC AGCGACTAGG CACATTTGCA GAGTATGAGG CCAAATGGAA CACCCAGAGG 
AAGACTACAG AACCAAGAGT ATCTGGCCGA TACCACTCTG CTGATAACAT CCTCGATACA 
GGAAAAGAGC GACGGAGTAA CCCCACCTGT GTGCATGAAA GATCTAGATC CTCACCTTCT 
GCTGACTTTT ATGGGCAGAA ACTTCCACCA CAAGAAAAGA AGTCAGCTGA CTGTTTCAAA 
TCTGAAAAGA GTCCTTGCCA ACAGGACAAG AATAGTACAA GTCAAAATTT CTACCCATCT 
GTTTCTTTGG AGCACAGCCA CTGGTTCTAT ACACCCAGCA GGTTAAATGA AAAAGGATAT 
AGTGAAATCT TATGCAAGGA GAAACCAGAA ACACCTCCTG AAGAACCTGA GAAAAAGACT 
GTAGATCCTC CCTCCAGCCA TCATAAGCCT GCACTACATT TCCCTGTCCA TAGCACTCGC 
AGTGAACCAT CAGCCCTCTC CAAAAACAAG AGCTCATTTC TCTTGCCCCA TCTGGAGAAG 
TACAAATGCC CTGAGGGAAC ACCTCCTCCT CTTAGCAATA TTCAGGAGGT CCAGCCTGGA 
TCACAAGGAG GAGCTCTGGC CCCAATTTCC CCAGTCAACA ATCAAAGCTC CGCCCCAGTT 
TCAGCCCCCA TTCCTTGGAA GAGTCCAGAG TACAGCAAAG GGCCTGTCGG GGAGGAGGAG 
CAACACCAAG AGCTTGTGCC TCCTCCCTTT CCACCTCCTC CCCCTCCCGT TGTCCTGCCC 
ACCCAACAAA CCGCTGGCCC GACCCAGCCC ACAATGGATG GGCAGCGTTC GCCCTCGCCC 
CAGTTCGCTC CCCAGAGGCT GACTGACAAG CCCCCTGTCT CCGTCTCCTC ACAGGATGAA 
GCTCCTGGAA GGATGGATCA GATTAAAGAT GAGAACACAT CTGTGAAGAA AGTGCCCATT 
AAGATAGTCC ATTCAGAGAG TGACACGGAA AAAGAAAGTC GGCAATATCT AGACCTGCCC 
AGCGAGACGC CTGTCAACTC GCAGGGACTT GTGGGAGCTC ATCTTCAAAG TTTGGGGAAC 
CCGGATCAGT CATACTCTTT GTTTTGTACA TACACCAGAC AAAAAGATCA GGTTCCTGGT 
CTTAGAGACC CAGACATGGG CCCTCTAAAA GACCAGGGGC CACAAAGCAA TATAAACCCA 
GTGTCTTATG TGGAACCTGG CCTTCAGACA GGTCCACCAT TGGATCAGAG TACCAATGGA 
GTGTCTTTGC ATCCTTCACA TTCAGAGGAT GATAAAAGAA AGGAGCTGGC TAGAGACATA 
ATGGACAAAG ACAAGTCCCT CGCTGACATT TTAGACCAGA GTAAGATGAA GACCACTATG 
GATCTGATGG AGGGGATTTT CCCTCAAGGA GATCAGCTAC TGGAGGAGGC CCAACAGCGT 
AGGAAGGCTC AACCTAAACC ACTCTCCCCT CGAAACTCTG TTGAGAAGAA AGAGGAAGAC 
AGCCTGGTGG CGGCCGCTGC TTTAGTGAGC AACTCAACCT ACTACAGCAC ATCTGCACCC 
AAAGCAGAGC TGCTGATCAA GATGAAGGAC ATGCAGGACC AGAGCGTTGA ACACAACTCA 
GAAGAGGAGC TGGAGGAGGA CAATGAAAGT GACCTCGCCT GCAAGAAGCA CGAGTTGATT 
GAAAGTCTCA GTAAGAAGTT GCAGGTCTTA AAAGAAGCTC AGGAGAGCCT GCAGGAGGAC 
GTGCAGGACA ACAACGCTCT TGGAGAAGAA GTGGAGGCCA TCGTACAGGG CGTCTGCAAG 
CCTAATGAAC TCGACAAATT CCGCATGTTC GTCGGGGATC TTGATAAAGT GGTCAACCTT 
CTGCTGTCTC TGTCAGGACG TTTGGCCAGG GTAGAGAATG CCCTGAACAG CCTGGAGGAA 
GATGCTTCGC TGGAGGAAAG GCGCACTCTG ACAGAGAAAC GCAAACTGCT GATTCGTCAG 
CACGAGGATG CTAAGGAGCT GAAGGAGAAC CTGGACAGGA GAGAGCGTGT GGTCTACGAC 
ATTCTGGCCA GTTACCTGCC TGAGGAGAGT ATGGCTGACT ACGAGCACTT TGTCAAGATG 
AAATCCGCTC TTATTATCGA GCAACGCAAG CTGGAGGACA AGATCAAACT GGGAGATGAG 
CAGCTCAAGT GTCTGATGGA CAGTCTCCCA ATGGACCAGA GAATAATCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356632.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212121.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa59471
Clone ID Related Accession (Same CDS sequence)	XM_017356632.2 , XM_017356632.1
Accession Version	XM_017356632.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAAAATGA AAATGGTGGA TATTGTAGCG CAGAAAATGC CATCAGAGAG CGACGTGCAT 
ATGGCCCGGA GCTTTCTCAC GAAGATTTTA CGAAGTTCCA TGAGAAAGAA TCGGTTTAAA 
GGGAGAAATG AACCCATGAG CAGACCTCAC TCCTGGCATG CCACCAAGTT CAACGAGACC 
CATTCAGAAG CCAAAACCCA GTCCACACCC TCACCAGTTT GGCAAACAAG ATATGATGCA 
AGTTCATTCT CTTCTGAACT CTCAACTGGC TGGGATCAAA CAAATCTACG GAGAGTATCC 
GATCAGTTCA GTTCTTTGGG GAGTATGGAC AGCCTAGAAC ATAGTTCTCA TCCCTATCCA 
CCTGGCCGTC TTTCCCCAAA CAAATCCAAC AATAATAGCA TTGAACATTT GGGTAGTGGT 
AAACGGGACT CGGCATACAG CTCTTTTTCT ACAAGTTCGG GAACCCCAGA TTACACACTG 
TCCAAAAGTA ACACGGCATC CACTGAGAAC ATGCTGTACA AAATAAATCA GTGGGATTCA 
AGTGCCAGGC ACAGCAATGG CAGGCACAGC CAAAACCTAA GCGAAGGGGT ACGACAAGAT 
GAGAGGCCTG GATACCTGCA CCACCTCTCA AGTACAGGTA ACCACGAGAT CCCAAGAGCT 
GAAGAACTGC CTGGCAACCG TCATTCAAGC TCTGGAAGAG TTAATATTGG ACCTGTCTGG 
CATGTTCCAG ATATGAAGAA AAACATGATA TCATCTACTC CACCTCCCAC TCCACCCACA 
CGCAGTGATA GCTTTGCAGC TACCAAGGTT CACGAGAAGG GTCTGATCAC AGGTACTTCA 
GAGGGCCTTG GTGGTCATTC TCAAGTAAAG CCTCAAGCGA AACCACAACA AAAGCCAGGA 
GAAACCCATG AAAGCACACA AAGGTCTAAC CAAGTCATCG AGATTTGTCT TGAGGGTCGA 
CGGGATTATA ATCTACAATC CAAAAATGAT TCCTCAAATA GCTGCATTTC ATCTGATTCT 
CATCATCAGT ATATCCAACG TACATCATCA GATAAGACAT ACTCCCTATC AACCACAGAC 
ATAAGAGATA GCCATCCATC CTATGGCCAT GCTCCATACC ATGCACAGCA GTACAGTGAC 
GAAGGCACTT TCCATGCTCA AACTAGAACA GCAATGAAAC CTCCATTCAG TGGCTATTTC 
AGTAGTATGC AGGAACTGCA CACAAACAAC CACATGCAAC TCAACAGCCA GAATCAAACT 
GGAAGACCAG CTGCATCACT GTCAGTTAAT TTAGGAGTCA ATTCCCACCA TATCTCTCAG 
GGATTAACAC AGGCTTCTTT AATGAGAGCT GATGACTCTA AAGCCTCTTC AGTCTCAGAA 
ATGTCCCACA ATGGACGGGA CAGAATGTCC ATAGGTTCTC AAGGAGGGAA TAAAGAGCGG 
TATTTCCCTC CTCAATCGCA GCACCATGAT AATAAAGAAA GAAGTGCCTC CTTTAAACAA 
AATGAAAACC AACATTATGT TTCTGCACCA AGTAATCCCT TGAATATGTC TGATTCAACA 
AAACATAATG AGCTTAGGGG ATGCCAAAAG CAGCACCATG TGCATGGCTC AGATGAGCTT 
TCTGGTAGTT ATCCACCCAG TAAGCAGCCC GAGCACAGGA GAAGTGCTGG CCATCTTCAT 
CATAAGGAAT ACTCGCAACA ACCCTTTCCA ACTAAAAGCG AATCAAAGAT ATGTCCCCAG 
AAAACACCTA TGTTGTATTC TCTGGCCCAA GGGCACAATG ACACAGATGA CTGTTCTAAT 
CAGATCAAAA GTGGAAGCGC ACAACATGAA GCCCTTGACA GTCTCAGTAG TAAACAGGTT 
AGACGGAATG ACCGATTTGC CACCACTTTG CGCAACGAGA TCCAGATGAG AAGGGCGCAT 
CTCCAAAAGA GTCAAAGTGC AGCCACTTTA GAAAGTCCAG TTGAAACTGT AGAAGAGTCT 
GCTTTGTGGA AGACCACAGG GACTGCTTCT CCCTCATCAG ATGGCTGCTT TTCCATTTCT 
TATAAAGACC ATCTTAAAGA AGCTCAGGCC AGAGTCCTCC AGGCTACATC ATTCAGGAGA 
AAAGACCTAG AGCCAGTATT ATTTGAGCAT CCAGGTACTG AAGGTCCCAC ACGAAAAGAT 
GTACCTCCTT CAAGTGTCTC AGAGGTCACA CCTAGCAAAC CCACCTCAGG GAGTAATCAT 
GTGCTTCGCA TTGGAGGCCG TAAGCGGATA TCTGCTGAAA AGAAAGTGAC GTCTTTTTCT 
GAGCCAGATA AAATTCATGA AGTAGGAGCT GATGAACGCT CCACTATGCC TGAAAATGTT 
GCACACTTAG AAAATCAACA AAGATTCTTT GAGACAACAG GGAAACCAGT TTTCCCTAAG 
CCTATGCCAA AGCAAAACCT GCAGATATCA GAAGACCCCA GACTGTCCAA GCCTGGAGGC 
ATGCACTATT CTGCAAACAG TGATATGGCA GGGCGGAATA ATGGTGAAAG CTCAACCCCC 
ATTGAACACC ACATTAATGA AGGTCTGGAT GGCCCTAACT CAGTGAACCA TCAAGCATTG 
CTAGAACAAC AGCGACTAGG CACATTTGCA GAGTATGAGG CCAAATGGAA CACCCAGAGG 
AAGACTACAG AACCAAGAGT ATCTGGCCGA TACCACTCTG CTGATAACAT CCTCGATACA 
GGAAAAGAGC GACGGAGTAA CCCCACCTGT GTGCATGAAA GATCTAGATC CTCACCTTCT 
GCTGACTTTT ATGGGCAGAA ACTTCCACCA CAAGAAAAGA AGTCAGCTGA CTGTTTCAAA 
TCTGAAAAGA GTCCTTGCCA ACAGGACAAG AATAGTACAA GTCAAAATTT CTACCCATCT 
GTTTCTTTGG AGCACAGCCA CTGGTTCTAT ACACCCAGCA GGTTAAATGA AAAAGGATAT 
AGTGAAATCT TATGCAAGGA GAAACCAGAA ACACCTCCTG AAGAACCTGA GAAAAAGACT 
GTAGATCCTC CCTCCAGCCA TCATAAGCCT GCACTACATT TCCCTGTCCA TAGCACTCGC 
AGTGAACCAT CAGCCCTCTC CAAAAACAAG AGCTCATTTC TCTTGCCCCA TCTGGAGAAG 
TACAAATGCC CTGAGGGAAC ACCTCCTCCT CTTAGCAATA TTCAGGAGGT CCAGCCTGGA 
TCACAAGGAG GAGCTCTGGC CCCAATTTCC CCAGTCAACA ATCAAAGCTC CGCCCCAGTT 
TCAGCCCCCA TTCCTTGGAA GAGTCCAGAG TACAGCAAAG GGCCTGTCGG GGAGGAGGAG 
CAACACCAAG AGCTTGTGCC TCCTCCCTTT CCACCTCCTC CCCCTCCCGT TGTCCTGCCC 
ACCCAACAAA CCGCTGGCCC GACCCAGCCC ACAATGGATG GGCAGCGTTC GCCCTCGCCC 
CAGTTCGCTC CCCAGAGGCT GACTGACAAG CCCCCTGTCT CCGTCTCCTC ACAGGATGAA 
GCTCCTGGAA GGATGGATCA GATTAAAGAT GAGAACACAT CTGTGAAGAA AGTGCCCATT 
AAGATAGTCC ATTCAGAGAG TGACACGGAA AAAGAAAGTC GGCAATATCT AGACCTGCCC 
AGCGAGACGC CTGTCAACTC GCAGGGACTT GTGGGAGCTC ATCTTCAAAG TTTGGGGAAC 
CCGGATCAGT CATACTCTTT GTTTTGTACA TACACCAGAC AAAAAGATCA GGTTCCTGGT 
CTTAGAGACC CAGACATGGG CCCTCTAAAA GACCAGGGGC CACAAAGCAA TATAAACCCA 
GTGTCTTATG TGGAACCTGG CCTTCAGACA GGTCCACCAT TGGATCAGAG TACCAATGGA 
GTGTCTTTGC ATCCTTCACA TTCAGAGGAT GATAAAAGAA AGGAGCTGGC TAGAGACATA 
ATGGACAAAG ACAAGTCCCT CGCTGACATT TTAGACCAGA GTAAGATGAA GACCACTATG 
GATCTGATGG AGGGGATTTT CCCTCAAGGA GATCAGCTAC TGGAGGAGGC CCAACAGCGT 
AGGAAGGCTC AACCTAAACC ACTCTCCCCT CGAAACTCTG TTGAGAAGAA AGAGGAAGAC 
AGCCTGGTGG CGGCCGCTGC TTTAGTGAGC AACTCAACCT ACTACAGCAC ATCTGCACCC 
AAAGCAGAGC TGCTGATCAA GATGAAGGAC ATGCAGGACC AGAGCGTTGA ACACAACTCA 
GAAGAGGAGC TGGAGGAGGA CAATGAAAGT GACCTCGCCT GCAAGAAGCA CGAGTTGATT 
GAAAGTCTCA GTAAGAAGTT GCAGGTCTTA AAAGAAGCTC AGGAGAGCCT GCAGGAGGAC 
GTGCAGGACA ACAACGCTCT TGGAGAAGAA GTGGAGGCCA TCGTACAGGG CGTCTGCAAG 
CCTAATGAAC TCGACAAATT CCGCATGTTC GTCGGGGATC TTGATAAAGT GGTCAACCTT 
CTGCTGTCTC TGTCAGGACG TTTGGCCAGG GTAGAGAATG CCCTGAACAG CCTGGAGGAA 
GATGCTTCGC TGGAGGAAAG GCGCACTCTG ACAGAGAAAC GCAAACTGCT GATTCGTCAG 
CACGAGGATG CTAAGGAGCT GAAGGAGAAC CTGGACAGGA GAGAGCGTGT GGTCTACGAC 
ATTCTGGCCA GTTACCTGCC TGAGGAGAGT ATGGCTGACT ACGAGCACTT TGTCAAGATG 
AAATCCGCTC TTATTATCGA GCAACGCAAG CTGGAGGACA AGATCAAACT GGGAGATGAG 
CAGCTCAAGT GTCTGATGGA CAGTCTCCCA ATGGACCAGA GAATAATCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212121.1
CDS	766..5919
Translation	MDTINQYPVNPSFPEDDHRSFCSSIRSASFNEHHVNDEGWKVVEVTLSGGAPWGFTLRGGLEHGEPLLITKVEEGSKAATVRLQVGDELININNISLTGFRQEAICLVKSSYKTLSLVVKRKMKMVDIVAQKMPSESDVHMARSFLTKILRSSMRKNRFKGRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356632.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356632.1

ORF Insert Sequence:

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAAAATGA AAATGGTGGA TATTGTAGCG CAGAAAATGC CATCAGAGAG CGACGTGCAT 
ATGGCCCGGA GCTTTCTCAC GAAGATTTTA CGAAGTTCCA TGAGAAAGAA TCGGTTTAAA 
GGGAGAAATG AACCCATGAG CAGACCTCAC TCCTGGCATG CCACCAAGTT CAACGAGACC 
CATTCAGAAG CCAAAACCCA GTCCACACCC TCACCAGTTT GGCAAACAAG ATATGATGCA 
AGTTCATTCT CTTCTGAACT CTCAACTGGC TGGGATCAAA CAAATCTACG GAGAGTATCC 
GATCAGTTCA GTTCTTTGGG GAGTATGGAC AGCCTAGAAC ATAGTTCTCA TCCCTATCCA 
CCTGGCCGTC TTTCCCCAAA CAAATCCAAC AATAATAGCA TTGAACATTT GGGTAGTGGT 
AAACGGGACT CGGCATACAG CTCTTTTTCT ACAAGTTCGG GAACCCCAGA TTACACACTG 
TCCAAAAGTA ACACGGCATC CACTGAGAAC ATGCTGTACA AAATAAATCA GTGGGATTCA 
AGTGCCAGGC ACAGCAATGG CAGGCACAGC CAAAACCTAA GCGAAGGGGT ACGACAAGAT 
GAGAGGCCTG GATACCTGCA CCACCTCTCA AGTACAGGTA ACCACGAGAT CCCAAGAGCT 
GAAGAACTGC CTGGCAACCG TCATTCAAGC TCTGGAAGAG TTAATATTGG ACCTGTCTGG 
CATGTTCCAG ATATGAAGAA AAACATGATA TCATCTACTC CACCTCCCAC TCCACCCACA 
CGCAGTGATA GCTTTGCAGC TACCAAGGTT CACGAGAAGG GTCTGATCAC AGGTACTTCA 
GAGGGCCTTG GTGGTCATTC TCAAGTAAAG CCTCAAGCGA AACCACAACA AAAGCCAGGA 
GAAACCCATG AAAGCACACA AAGGTCTAAC CAAGTCATCG AGATTTGTCT TGAGGGTCGA 
CGGGATTATA ATCTACAATC CAAAAATGAT TCCTCAAATA GCTGCATTTC ATCTGATTCT 
CATCATCAGT ATATCCAACG TACATCATCA GATAAGACAT ACTCCCTATC AACCACAGAC 
ATAAGAGATA GCCATCCATC CTATGGCCAT GCTCCATACC ATGCACAGCA GTACAGTGAC 
GAAGGCACTT TCCATGCTCA AACTAGAACA GCAATGAAAC CTCCATTCAG TGGCTATTTC 
AGTAGTATGC AGGAACTGCA CACAAACAAC CACATGCAAC TCAACAGCCA GAATCAAACT 
GGAAGACCAG CTGCATCACT GTCAGTTAAT TTAGGAGTCA ATTCCCACCA TATCTCTCAG 
GGATTAACAC AGGCTTCTTT AATGAGAGCT GATGACTCTA AAGCCTCTTC AGTCTCAGAA 
ATGTCCCACA ATGGACGGGA CAGAATGTCC ATAGGTTCTC AAGGAGGGAA TAAAGAGCGG 
TATTTCCCTC CTCAATCGCA GCACCATGAT AATAAAGAAA GAAGTGCCTC CTTTAAACAA 
AATGAAAACC AACATTATGT TTCTGCACCA AGTAATCCCT TGAATATGTC TGATTCAACA 
AAACATAATG AGCTTAGGGG ATGCCAAAAG CAGCACCATG TGCATGGCTC AGATGAGCTT 
TCTGGTAGTT ATCCACCCAG TAAGCAGCCC GAGCACAGGA GAAGTGCTGG CCATCTTCAT 
CATAAGGAAT ACTCGCAACA ACCCTTTCCA ACTAAAAGCG AATCAAAGAT ATGTCCCCAG 
AAAACACCTA TGTTGTATTC TCTGGCCCAA GGGCACAATG ACACAGATGA CTGTTCTAAT 
CAGATCAAAA GTGGAAGCGC ACAACATGAA GCCCTTGACA GTCTCAGTAG TAAACAGGTT 
AGACGGAATG ACCGATTTGC CACCACTTTG CGCAACGAGA TCCAGATGAG AAGGGCGCAT 
CTCCAAAAGA GTCAAAGTGC AGCCACTTTA GAAAGTCCAG TTGAAACTGT AGAAGAGTCT 
GCTTTGTGGA AGACCACAGG GACTGCTTCT CCCTCATCAG ATGGCTGCTT TTCCATTTCT 
TATAAAGACC ATCTTAAAGA AGCTCAGGCC AGAGTCCTCC AGGCTACATC ATTCAGGAGA 
AAAGACCTAG AGCCAGTATT ATTTGAGCAT CCAGGTACTG AAGGTCCCAC ACGAAAAGAT 
GTACCTCCTT CAAGTGTCTC AGAGGTCACA CCTAGCAAAC CCACCTCAGG GAGTAATCAT 
GTGCTTCGCA TTGGAGGCCG TAAGCGGATA TCTGCTGAAA AGAAAGTGAC GTCTTTTTCT 
GAGCCAGATA AAATTCATGA AGTAGGAGCT GATGAACGCT CCACTATGCC TGAAAATGTT 
GCACACTTAG AAAATCAACA AAGATTCTTT GAGACAACAG GGAAACCAGT TTTCCCTAAG 
CCTATGCCAA AGCAAAACCT GCAGATATCA GAAGACCCCA GACTGTCCAA GCCTGGAGGC 
ATGCACTATT CTGCAAACAG TGATATGGCA GGGCGGAATA ATGGTGAAAG CTCAACCCCC 
ATTGAACACC ACATTAATGA AGGTCTGGAT GGCCCTAACT CAGTGAACCA TCAAGCATTG 
CTAGAACAAC AGCGACTAGG CACATTTGCA GAGTATGAGG CCAAATGGAA CACCCAGAGG 
AAGACTACAG AACCAAGAGT ATCTGGCCGA TACCACTCTG CTGATAACAT CCTCGATACA 
GGAAAAGAGC GACGGAGTAA CCCCACCTGT GTGCATGAAA GATCTAGATC CTCACCTTCT 
GCTGACTTTT ATGGGCAGAA ACTTCCACCA CAAGAAAAGA AGTCAGCTGA CTGTTTCAAA 
TCTGAAAAGA GTCCTTGCCA ACAGGACAAG AATAGTACAA GTCAAAATTT CTACCCATCT 
GTTTCTTTGG AGCACAGCCA CTGGTTCTAT ACACCCAGCA GGTTAAATGA AAAAGGATAT 
AGTGAAATCT TATGCAAGGA GAAACCAGAA ACACCTCCTG AAGAACCTGA GAAAAAGACT 
GTAGATCCTC CCTCCAGCCA TCATAAGCCT GCACTACATT TCCCTGTCCA TAGCACTCGC 
AGTGAACCAT CAGCCCTCTC CAAAAACAAG AGCTCATTTC TCTTGCCCCA TCTGGAGAAG 
TACAAATGCC CTGAGGGAAC ACCTCCTCCT CTTAGCAATA TTCAGGAGGT CCAGCCTGGA 
TCACAAGGAG GAGCTCTGGC CCCAATTTCC CCAGTCAACA ATCAAAGCTC CGCCCCAGTT 
TCAGCCCCCA TTCCTTGGAA GAGTCCAGAG TACAGCAAAG GGCCTGTCGG GGAGGAGGAG 
CAACACCAAG AGCTTGTGCC TCCTCCCTTT CCACCTCCTC CCCCTCCCGT TGTCCTGCCC 
ACCCAACAAA CCGCTGGCCC GACCCAGCCC ACAATGGATG GGCAGCGTTC GCCCTCGCCC 
CAGTTCGCTC CCCAGAGGCT GACTGACAAG CCCCCTGTCT CCGTCTCCTC ACAGGATGAA 
GCTCCTGGAA GGATGGATCA GATTAAAGAT GAGAACACAT CTGTGAAGAA AGTGCCCATT 
AAGATAGTCC ATTCAGAGAG TGACACGGAA AAAGAAAGTC GGCAATATCT AGACCTGCCC 
AGCGAGACGC CTGTCAACTC GCAGGGACTT GTGGGAGCTC ATCTTCAAAG TTTGGGGAAC 
CCGGATCAGT CATACTCTTT GTTTTGTACA TACACCAGAC AAAAAGATCA GGTTCCTGGT 
CTTAGAGACC CAGACATGGG CCCTCTAAAA GACCAGGGGC CACAAAGCAA TATAAACCCA 
GTGTCTTATG TGGAACCTGG CCTTCAGACA GGTCCACCAT TGGATCAGAG TACCAATGGA 
GTGTCTTTGC ATCCTTCACA TTCAGAGGAT GATAAAAGAA AGGAGCTGGC TAGAGACATA 
ATGGACAAAG ACAAGTCCCT CGCTGACATT TTAGACCAGA GTAAGATGAA GACCACTATG 
GATCTGATGG AGGGGATTTT CCCTCAAGGA GATCAGCTAC TGGAGGAGGC CCAACAGCGT 
AGGAAGGCTC AACCTAAACC ACTCTCCCCT CGAAACTCTG TTGAGAAGAA AGAGGAAGAC 
AGCCTGGTGG CGGCCGCTGC TTTAGTGAGC AACTCAACCT ACTACAGCAC ATCTGCACCC 
AAAGCAGAGC TGCTGATCAA GATGAAGGAC ATGCAGGACC AGAGCGTTGA ACACAACTCA 
GAAGAGGAGC TGGAGGAGGA CAATGAAAGT GACCTCGCCT GCAAGAAGCA CGAGTTGATT 
GAAAGTCTCA GTAAGAAGTT GCAGGTCTTA AAAGAAGCTC AGGAGAGCCT GCAGGAGGAC 
GTGCAGGACA ACAACGCTCT TGGAGAAGAA GTGGAGGCCA TCGTACAGGG CGTCTGCAAG 
CCTAATGAAC TCGACAAATT CCGCATGTTC GTCGGGGATC TTGATAAAGT GGTCAACCTT 
CTGCTGTCTC TGTCAGGACG TTTGGCCAGG GTAGAGAATG CCCTGAACAG CCTGGAGGAA 
GATGCTTCGC TGGAGGAAAG GCGCACTCTG ACAGAGAAAC GCAAACTGCT GATTCGTCAG 
CACGAGGATG CTAAGGAGCT GAAGGAGAAC CTGGACAGGA GAGAGCGTGT GGTCTACGAC 
ATTCTGGCCA GTTACCTGCC TGAGGAGAGT ATGGCTGACT ACGAGCACTT TGTCAAGATG 
AAATCCGCTC TTATTATCGA GCAACGCAAG CTGGAGGACA AGATCAAACT GGGAGATGAG 
CAGCTCAAGT GTCTGATGGA CAGTCTCCCA ATGGACCAGA GAATAATCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356633.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212122.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X2, mRNA.

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CloneID	ODa59472
Clone ID Related Accession (Same CDS sequence)	XM_017356633.1 , XM_017356633.2
Accession Version	XM_017356633.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAAAATGA AAATGGTGGA TATTGTAGCG CAGAAAATGC CATCAGAGAG CGACGTGCAT 
ATGGCCCGGA GCTTTCTCAC GAAGATTTTA CGAAGTTCCA TGAGGAGAAA TGAACCCATG 
AGCAGACCTC ACTCCTGGCA TGCCACCAAG TTCAACGAGA CCCATTCAGA AGCCAAAACC 
CAGTCCACAC CCTCACCAGT TTGGCAAACA AGATATGATG CAAGTTCATT CTCTTCTGAA 
CTCTCAACTG GCTGGGATCA AACAAATCTA CGGAGAGTAT CCGATCAGTT CAGTTCTTTG 
GGGAGTATGG ACAGCCTAGA ACATAGTTCT CATCCCTATC CACCTGGCCG TCTTTCCCCA 
AACAAATCCA ACAATAATAG CATTGAACAT TTGGGTAGTG GTAAACGGGA CTCGGCATAC 
AGCTCTTTTT CTACAAGTTC GGGAACCCCA GATTACACAC TGTCCAAAAG TAACACGGCA 
TCCACTGAGA ACATGCTGTA CAAAATAAAT CAGTGGGATT CAAGTGCCAG GCACAGCAAT 
GGCAGGCACA GCCAAAACCT AAGCGAAGGG GTACGACAAG ATGAGAGGCC TGGATACCTG 
CACCACCTCT CAAGTACAGG TAACCACGAG ATCCCAAGAG CTGAAGAACT GCCTGGCAAC 
CGTCATTCAA GCTCTGGAAG AGTTAATATT GGACCTGTCT GGCATGTTCC AGATATGAAG 
AAAAACATGA TATCATCTAC TCCACCTCCC ACTCCACCCA CACGCAGTGA TAGCTTTGCA 
GCTACCAAGG TTCACGAGAA GGGTCTGATC ACAGGTACTT CAGAGGGCCT TGGTGGTCAT 
TCTCAAGTAA AGCCTCAAGC GAAACCACAA CAAAAGCCAG GAGAAACCCA TGAAAGCACA 
CAAAGGTCTA ACCAAGTCAT CGAGATTTGT CTTGAGGGTC GACGGGATTA TAATCTACAA 
TCCAAAAATG ATTCCTCAAA TAGCTGCATT TCATCTGATT CTCATCATCA GTATATCCAA 
CGTACATCAT CAGATAAGAC ATACTCCCTA TCAACCACAG ACATAAGAGA TAGCCATCCA 
TCCTATGGCC ATGCTCCATA CCATGCACAG CAGTACAGTG ACGAAGGCAC TTTCCATGCT 
CAAACTAGAA CAGCAATGAA ACCTCCATTC AGTGGCTATT TCAGTAGTAT GCAGGAACTG 
CACACAAACA ACCACATGCA ACTCAACAGC CAGAATCAAA CTGGAAGACC AGCTGCATCA 
CTGTCAGTTA ATTTAGGAGT CAATTCCCAC CATATCTCTC AGGGATTAAC ACAGGCTTCT 
TTAATGAGAG CTGATGACTC TAAAGCCTCT TCAGTCTCAG AAATGTCCCA CAATGGACGG 
GACAGAATGT CCATAGGTTC TCAAGGAGGG AATAAAGAGC GGTATTTCCC TCCTCAATCG 
CAGCACCATG ATAATAAAGA AAGAAGTGCC TCCTTTAAAC AAAATGAAAA CCAACATTAT 
GTTTCTGCAC CAAGTAATCC CTTGAATATG TCTGATTCAA CAAAACATAA TGAGCTTAGG 
GGATGCCAAA AGCAGCACCA TGTGCATGGC TCAGATGAGC TTTCTGGTAG TTATCCACCC 
AGTAAGCAGC CCGAGCACAG GAGAAGTGCT GGCCATCTTC ATCATAAGGA ATACTCGCAA 
CAACCCTTTC CAACTAAAAG CGAATCAAAG ATATGTCCCC AGAAAACACC TATGTTGTAT 
TCTCTGGCCC AAGGGCACAA TGACACAGAT GACTGTTCTA ATCAGATCAA AAGTGGAAGC 
GCACAACATG AAGCCCTTGA CAGTCTCAGT AGTAAACAGG TTAGACGGAA TGACCGATTT 
GCCACCACTT TGCGCAACGA GATCCAGATG AGAAGGGCGC ATCTCCAAAA GAGTCAAAGT 
GCAGCCACTT TAGAAAGTCC AGTTGAAACT GTAGAAGAGT CTGCTTTGTG GAAGACCACA 
GGGACTGCTT CTCCCTCATC AGATGGCTGC TTTTCCATTT CTTATAAAGA CCATCTTAAA 
GAAGCTCAGG CCAGAGTCCT CCAGGCTACA TCATTCAGGA GAAAAGACCT AGAGCCAGTA 
TTATTTGAGC ATCCAGGTAC TGAAGGTCCC ACACGAAAAG ATGTACCTCC TTCAAGTGTC 
TCAGAGGTCA CACCTAGCAA ACCCACCTCA GGGAGTAATC ATGTGCTTCG CATTGGAGGC 
CGTAAGCGGA TATCTGCTGA AAAGAAAGTG ACGTCTTTTT CTGAGCCAGA TAAAATTCAT 
GAAGTAGGAG CTGATGAACG CTCCACTATG CCTGAAAATG TTGCACACTT AGAAAATCAA 
CAAAGATTCT TTGAGACAAC AGGGAAACCA GTTTTCCCTA AGCCTATGCC AAAGCAAAAC 
CTGCAGATAT CAGAAGACCC CAGACTGTCC AAGCCTGGAG GCATGCACTA TTCTGCAAAC 
AGTGATATGG CAGGGCGGAA TAATGGTGAA AGCTCAACCC CCATTGAACA CCACATTAAT 
GAAGGTCTGG ATGGCCCTAA CTCAGTGAAC CATCAAGCAT TGCTAGAACA ACAGCGACTA 
GGCACATTTG CAGAGTATGA GGCCAAATGG AACACCCAGA GGAAGACTAC AGAACCAAGA 
GTATCTGGCC GATACCACTC TGCTGATAAC ATCCTCGATA CAGGAAAAGA GCGACGGAGT 
AACCCCACCT GTGTGCATGA AAGATCTAGA TCCTCACCTT CTGCTGACTT TTATGGGCAG 
AAACTTCCAC CACAAGAAAA GAAGTCAGCT GACTGTTTCA AATCTGAAAA GAGTCCTTGC 
CAACAGGACA AGAATAGTAC AAGTCAAAAT TTCTACCCAT CTGTTTCTTT GGAGCACAGC 
CACTGGTTCT ATACACCCAG CAGGTTAAAT GAAAAAGGAT ATAGTGAAAT CTTATGCAAG 
GAGAAACCAG AAACACCTCC TGAAGAACCT GAGAAAAAGA CTGTAGATCC TCCCTCCAGC 
CATCATAAGC CTGCACTACA TTTCCCTGTC CATAGCACTC GCAGTGAACC ATCAGCCCTC 
TCCAAAAACA AGAGCTCATT TCTCTTGCCC CATCTGGAGA AGTACAAATG CCCTGAGGGA 
ACACCTCCTC CTCTTAGCAA TATTCAGGAG GTCCAGCCTG GATCACAAGG AGGAGCTCTG 
GCCCCAATTT CCCCAGTCAA CAATCAAAGC TCCGCCCCAG TTTCAGCCCC CATTCCTTGG 
AAGAGTCCAG AGTACAGCAA AGGGCCTGTC GGGGAGGAGG AGCAACACCA AGAGCTTGTG 
CCTCCTCCCT TTCCACCTCC TCCCCCTCCC GTTGTCCTGC CCACCCAACA AACCGCTGGC 
CCGACCCAGC CCACAATGGA TGGGCAGCGT TCGCCCTCGC CCCAGTTCGC TCCCCAGAGG 
CTGACTGACA AGCCCCCTGT CTCCGTCTCC TCACAGGATG AAGCTCCTGG AAGGATGGAT 
CAGATTAAAG ATGAGAACAC ATCTGTGAAG AAAGTGCCCA TTAAGATAGT CCATTCAGAG 
AGTGACACGG AAAAAGAAAG TCGGCAATAT CTAGACCTGC CCAGCGAGAC GCCTGTCAAC 
TCGCAGGGAC TTGTGGGAGC TCATCTTCAA AGTTTGGGGA ACCCGGATCA GTCATACTCT 
TTGTTTTGTA CATACACCAG ACAAAAAGAT CAGGTTCCTG GTCTTAGAGA CCCAGACATG 
GGCCCTCTAA AAGACCAGGG GCCACAAAGC AATATAAACC CAGTGTCTTA TGTGGAACCT 
GGCCTTCAGA CAGGTCCACC ATTGGATCAG AGTACCAATG GAGTGTCTTT GCATCCTTCA 
CATTCAGAGG ATGATAAAAG AAAGGAGCTG GCTAGAGACA TAATGGACAA AGACAAGTCC 
CTCGCTGACA TTTTAGACCA GAGTAAGATG AAGACCACTA TGGATCTGAT GGAGGGGATT 
TTCCCTCAAG GAGATCAGCT ACTGGAGGAG GCCCAACAGC GTAGGAAGGC TCAACCTAAA 
CCACTCTCCC CTCGAAACTC TGTTGAGAAG AAAGAGGAAG ACAGCCTGGT GGCGGCCGCT 
GCTTTAGTGA GCAACTCAAC CTACTACAGC ACATCTGCAC CCAAAGCAGA GCTGCTGATC 
AAGATGAAGG ACATGCAGGA CCAGAGCGTT GAACACAACT CAGAAGAGGA GCTGGAGGAG 
GACAATGAAA GTGACCTCGC CTGCAAGAAG CACGAGTTGA TTGAAAGTCT CAGTAAGAAG 
TTGCAGGTCT TAAAAGAAGC TCAGGAGAGC CTGCAGGAGG ACGTGCAGGA CAACAACGCT 
CTTGGAGAAG AAGTGGAGGC CATCGTACAG GGCGTCTGCA AGCCTAATGA ACTCGACAAA 
TTCCGCATGT TCGTCGGGGA TCTTGATAAA GTGGTCAACC TTCTGCTGTC TCTGTCAGGA 
CGTTTGGCCA GGGTAGAGAA TGCCCTGAAC AGCCTGGAGG AAGATGCTTC GCTGGAGGAA 
AGGCGCACTC TGACAGAGAA ACGCAAACTG CTGATTCGTC AGCACGAGGA TGCTAAGGAG 
CTGAAGGAGA ACCTGGACAG GAGAGAGCGT GTGGTCTACG ACATTCTGGC CAGTTACCTG 
CCTGAGGAGA GTATGGCTGA CTACGAGCAC TTTGTCAAGA TGAAATCCGC TCTTATTATC 
GAGCAACGCA AGCTGGAGGA CAAGATCAAA CTGGGAGATG AGCAGCTCAA GTGTCTGATG 
GACAGTCTCC CAATGGACCA GAGAATAATC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212122.1
CDS	766..5901
Translation	MDTINQYPVNPSFPEDDHRSFCSSIRSASFNEHHVNDEGWKVVEVTLSGGAPWGFTLRGGLEHGEPLLITKVEEGSKAATVRLQVGDELININNISLTGFRQEAICLVKSSYKTLSLVVKRKMKMVDIVAQKMPSESDVHMARSFLTKILRSSMRRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356633.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356633.1

ORF Insert Sequence:

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAAAATGA AAATGGTGGA TATTGTAGCG CAGAAAATGC CATCAGAGAG CGACGTGCAT 
ATGGCCCGGA GCTTTCTCAC GAAGATTTTA CGAAGTTCCA TGAGGAGAAA TGAACCCATG 
AGCAGACCTC ACTCCTGGCA TGCCACCAAG TTCAACGAGA CCCATTCAGA AGCCAAAACC 
CAGTCCACAC CCTCACCAGT TTGGCAAACA AGATATGATG CAAGTTCATT CTCTTCTGAA 
CTCTCAACTG GCTGGGATCA AACAAATCTA CGGAGAGTAT CCGATCAGTT CAGTTCTTTG 
GGGAGTATGG ACAGCCTAGA ACATAGTTCT CATCCCTATC CACCTGGCCG TCTTTCCCCA 
AACAAATCCA ACAATAATAG CATTGAACAT TTGGGTAGTG GTAAACGGGA CTCGGCATAC 
AGCTCTTTTT CTACAAGTTC GGGAACCCCA GATTACACAC TGTCCAAAAG TAACACGGCA 
TCCACTGAGA ACATGCTGTA CAAAATAAAT CAGTGGGATT CAAGTGCCAG GCACAGCAAT 
GGCAGGCACA GCCAAAACCT AAGCGAAGGG GTACGACAAG ATGAGAGGCC TGGATACCTG 
CACCACCTCT CAAGTACAGG TAACCACGAG ATCCCAAGAG CTGAAGAACT GCCTGGCAAC 
CGTCATTCAA GCTCTGGAAG AGTTAATATT GGACCTGTCT GGCATGTTCC AGATATGAAG 
AAAAACATGA TATCATCTAC TCCACCTCCC ACTCCACCCA CACGCAGTGA TAGCTTTGCA 
GCTACCAAGG TTCACGAGAA GGGTCTGATC ACAGGTACTT CAGAGGGCCT TGGTGGTCAT 
TCTCAAGTAA AGCCTCAAGC GAAACCACAA CAAAAGCCAG GAGAAACCCA TGAAAGCACA 
CAAAGGTCTA ACCAAGTCAT CGAGATTTGT CTTGAGGGTC GACGGGATTA TAATCTACAA 
TCCAAAAATG ATTCCTCAAA TAGCTGCATT TCATCTGATT CTCATCATCA GTATATCCAA 
CGTACATCAT CAGATAAGAC ATACTCCCTA TCAACCACAG ACATAAGAGA TAGCCATCCA 
TCCTATGGCC ATGCTCCATA CCATGCACAG CAGTACAGTG ACGAAGGCAC TTTCCATGCT 
CAAACTAGAA CAGCAATGAA ACCTCCATTC AGTGGCTATT TCAGTAGTAT GCAGGAACTG 
CACACAAACA ACCACATGCA ACTCAACAGC CAGAATCAAA CTGGAAGACC AGCTGCATCA 
CTGTCAGTTA ATTTAGGAGT CAATTCCCAC CATATCTCTC AGGGATTAAC ACAGGCTTCT 
TTAATGAGAG CTGATGACTC TAAAGCCTCT TCAGTCTCAG AAATGTCCCA CAATGGACGG 
GACAGAATGT CCATAGGTTC TCAAGGAGGG AATAAAGAGC GGTATTTCCC TCCTCAATCG 
CAGCACCATG ATAATAAAGA AAGAAGTGCC TCCTTTAAAC AAAATGAAAA CCAACATTAT 
GTTTCTGCAC CAAGTAATCC CTTGAATATG TCTGATTCAA CAAAACATAA TGAGCTTAGG 
GGATGCCAAA AGCAGCACCA TGTGCATGGC TCAGATGAGC TTTCTGGTAG TTATCCACCC 
AGTAAGCAGC CCGAGCACAG GAGAAGTGCT GGCCATCTTC ATCATAAGGA ATACTCGCAA 
CAACCCTTTC CAACTAAAAG CGAATCAAAG ATATGTCCCC AGAAAACACC TATGTTGTAT 
TCTCTGGCCC AAGGGCACAA TGACACAGAT GACTGTTCTA ATCAGATCAA AAGTGGAAGC 
GCACAACATG AAGCCCTTGA CAGTCTCAGT AGTAAACAGG TTAGACGGAA TGACCGATTT 
GCCACCACTT TGCGCAACGA GATCCAGATG AGAAGGGCGC ATCTCCAAAA GAGTCAAAGT 
GCAGCCACTT TAGAAAGTCC AGTTGAAACT GTAGAAGAGT CTGCTTTGTG GAAGACCACA 
GGGACTGCTT CTCCCTCATC AGATGGCTGC TTTTCCATTT CTTATAAAGA CCATCTTAAA 
GAAGCTCAGG CCAGAGTCCT CCAGGCTACA TCATTCAGGA GAAAAGACCT AGAGCCAGTA 
TTATTTGAGC ATCCAGGTAC TGAAGGTCCC ACACGAAAAG ATGTACCTCC TTCAAGTGTC 
TCAGAGGTCA CACCTAGCAA ACCCACCTCA GGGAGTAATC ATGTGCTTCG CATTGGAGGC 
CGTAAGCGGA TATCTGCTGA AAAGAAAGTG ACGTCTTTTT CTGAGCCAGA TAAAATTCAT 
GAAGTAGGAG CTGATGAACG CTCCACTATG CCTGAAAATG TTGCACACTT AGAAAATCAA 
CAAAGATTCT TTGAGACAAC AGGGAAACCA GTTTTCCCTA AGCCTATGCC AAAGCAAAAC 
CTGCAGATAT CAGAAGACCC CAGACTGTCC AAGCCTGGAG GCATGCACTA TTCTGCAAAC 
AGTGATATGG CAGGGCGGAA TAATGGTGAA AGCTCAACCC CCATTGAACA CCACATTAAT 
GAAGGTCTGG ATGGCCCTAA CTCAGTGAAC CATCAAGCAT TGCTAGAACA ACAGCGACTA 
GGCACATTTG CAGAGTATGA GGCCAAATGG AACACCCAGA GGAAGACTAC AGAACCAAGA 
GTATCTGGCC GATACCACTC TGCTGATAAC ATCCTCGATA CAGGAAAAGA GCGACGGAGT 
AACCCCACCT GTGTGCATGA AAGATCTAGA TCCTCACCTT CTGCTGACTT TTATGGGCAG 
AAACTTCCAC CACAAGAAAA GAAGTCAGCT GACTGTTTCA AATCTGAAAA GAGTCCTTGC 
CAACAGGACA AGAATAGTAC AAGTCAAAAT TTCTACCCAT CTGTTTCTTT GGAGCACAGC 
CACTGGTTCT ATACACCCAG CAGGTTAAAT GAAAAAGGAT ATAGTGAAAT CTTATGCAAG 
GAGAAACCAG AAACACCTCC TGAAGAACCT GAGAAAAAGA CTGTAGATCC TCCCTCCAGC 
CATCATAAGC CTGCACTACA TTTCCCTGTC CATAGCACTC GCAGTGAACC ATCAGCCCTC 
TCCAAAAACA AGAGCTCATT TCTCTTGCCC CATCTGGAGA AGTACAAATG CCCTGAGGGA 
ACACCTCCTC CTCTTAGCAA TATTCAGGAG GTCCAGCCTG GATCACAAGG AGGAGCTCTG 
GCCCCAATTT CCCCAGTCAA CAATCAAAGC TCCGCCCCAG TTTCAGCCCC CATTCCTTGG 
AAGAGTCCAG AGTACAGCAA AGGGCCTGTC GGGGAGGAGG AGCAACACCA AGAGCTTGTG 
CCTCCTCCCT TTCCACCTCC TCCCCCTCCC GTTGTCCTGC CCACCCAACA AACCGCTGGC 
CCGACCCAGC CCACAATGGA TGGGCAGCGT TCGCCCTCGC CCCAGTTCGC TCCCCAGAGG 
CTGACTGACA AGCCCCCTGT CTCCGTCTCC TCACAGGATG AAGCTCCTGG AAGGATGGAT 
CAGATTAAAG ATGAGAACAC ATCTGTGAAG AAAGTGCCCA TTAAGATAGT CCATTCAGAG 
AGTGACACGG AAAAAGAAAG TCGGCAATAT CTAGACCTGC CCAGCGAGAC GCCTGTCAAC 
TCGCAGGGAC TTGTGGGAGC TCATCTTCAA AGTTTGGGGA ACCCGGATCA GTCATACTCT 
TTGTTTTGTA CATACACCAG ACAAAAAGAT CAGGTTCCTG GTCTTAGAGA CCCAGACATG 
GGCCCTCTAA AAGACCAGGG GCCACAAAGC AATATAAACC CAGTGTCTTA TGTGGAACCT 
GGCCTTCAGA CAGGTCCACC ATTGGATCAG AGTACCAATG GAGTGTCTTT GCATCCTTCA 
CATTCAGAGG ATGATAAAAG AAAGGAGCTG GCTAGAGACA TAATGGACAA AGACAAGTCC 
CTCGCTGACA TTTTAGACCA GAGTAAGATG AAGACCACTA TGGATCTGAT GGAGGGGATT 
TTCCCTCAAG GAGATCAGCT ACTGGAGGAG GCCCAACAGC GTAGGAAGGC TCAACCTAAA 
CCACTCTCCC CTCGAAACTC TGTTGAGAAG AAAGAGGAAG ACAGCCTGGT GGCGGCCGCT 
GCTTTAGTGA GCAACTCAAC CTACTACAGC ACATCTGCAC CCAAAGCAGA GCTGCTGATC 
AAGATGAAGG ACATGCAGGA CCAGAGCGTT GAACACAACT CAGAAGAGGA GCTGGAGGAG 
GACAATGAAA GTGACCTCGC CTGCAAGAAG CACGAGTTGA TTGAAAGTCT CAGTAAGAAG 
TTGCAGGTCT TAAAAGAAGC TCAGGAGAGC CTGCAGGAGG ACGTGCAGGA CAACAACGCT 
CTTGGAGAAG AAGTGGAGGC CATCGTACAG GGCGTCTGCA AGCCTAATGA ACTCGACAAA 
TTCCGCATGT TCGTCGGGGA TCTTGATAAA GTGGTCAACC TTCTGCTGTC TCTGTCAGGA 
CGTTTGGCCA GGGTAGAGAA TGCCCTGAAC AGCCTGGAGG AAGATGCTTC GCTGGAGGAA 
AGGCGCACTC TGACAGAGAA ACGCAAACTG CTGATTCGTC AGCACGAGGA TGCTAAGGAG 
CTGAAGGAGA ACCTGGACAG GAGAGAGCGT GTGGTCTACG ACATTCTGGC CAGTTACCTG 
CCTGAGGAGA GTATGGCTGA CTACGAGCAC TTTGTCAAGA TGAAATCCGC TCTTATTATC 
GAGCAACGCA AGCTGGAGGA CAAGATCAAA CTGGGAGATG AGCAGCTCAA GTGTCTGATG 
GACAGTCTCC CAATGGACCA GAGAATAATC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356633.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212122.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X2, mRNA.

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CloneID	ODa59472
Clone ID Related Accession (Same CDS sequence)	XM_017356633.1 , XM_017356633.2
Accession Version	XM_017356633.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356633.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAAAATGA AAATGGTGGA TATTGTAGCG CAGAAAATGC CATCAGAGAG CGACGTGCAT 
ATGGCCCGGA GCTTTCTCAC GAAGATTTTA CGAAGTTCCA TGAGGAGAAA TGAACCCATG 
AGCAGACCTC ACTCCTGGCA TGCCACCAAG TTCAACGAGA CCCATTCAGA AGCCAAAACC 
CAGTCCACAC CCTCACCAGT TTGGCAAACA AGATATGATG CAAGTTCATT CTCTTCTGAA 
CTCTCAACTG GCTGGGATCA AACAAATCTA CGGAGAGTAT CCGATCAGTT CAGTTCTTTG 
GGGAGTATGG ACAGCCTAGA ACATAGTTCT CATCCCTATC CACCTGGCCG TCTTTCCCCA 
AACAAATCCA ACAATAATAG CATTGAACAT TTGGGTAGTG GTAAACGGGA CTCGGCATAC 
AGCTCTTTTT CTACAAGTTC GGGAACCCCA GATTACACAC TGTCCAAAAG TAACACGGCA 
TCCACTGAGA ACATGCTGTA CAAAATAAAT CAGTGGGATT CAAGTGCCAG GCACAGCAAT 
GGCAGGCACA GCCAAAACCT AAGCGAAGGG GTACGACAAG ATGAGAGGCC TGGATACCTG 
CACCACCTCT CAAGTACAGG TAACCACGAG ATCCCAAGAG CTGAAGAACT GCCTGGCAAC 
CGTCATTCAA GCTCTGGAAG AGTTAATATT GGACCTGTCT GGCATGTTCC AGATATGAAG 
AAAAACATGA TATCATCTAC TCCACCTCCC ACTCCACCCA CACGCAGTGA TAGCTTTGCA 
GCTACCAAGG TTCACGAGAA GGGTCTGATC ACAGGTACTT CAGAGGGCCT TGGTGGTCAT 
TCTCAAGTAA AGCCTCAAGC GAAACCACAA CAAAAGCCAG GAGAAACCCA TGAAAGCACA 
CAAAGGTCTA ACCAAGTCAT CGAGATTTGT CTTGAGGGTC GACGGGATTA TAATCTACAA 
TCCAAAAATG ATTCCTCAAA TAGCTGCATT TCATCTGATT CTCATCATCA GTATATCCAA 
CGTACATCAT CAGATAAGAC ATACTCCCTA TCAACCACAG ACATAAGAGA TAGCCATCCA 
TCCTATGGCC ATGCTCCATA CCATGCACAG CAGTACAGTG ACGAAGGCAC TTTCCATGCT 
CAAACTAGAA CAGCAATGAA ACCTCCATTC AGTGGCTATT TCAGTAGTAT GCAGGAACTG 
CACACAAACA ACCACATGCA ACTCAACAGC CAGAATCAAA CTGGAAGACC AGCTGCATCA 
CTGTCAGTTA ATTTAGGAGT CAATTCCCAC CATATCTCTC AGGGATTAAC ACAGGCTTCT 
TTAATGAGAG CTGATGACTC TAAAGCCTCT TCAGTCTCAG AAATGTCCCA CAATGGACGG 
GACAGAATGT CCATAGGTTC TCAAGGAGGG AATAAAGAGC GGTATTTCCC TCCTCAATCG 
CAGCACCATG ATAATAAAGA AAGAAGTGCC TCCTTTAAAC AAAATGAAAA CCAACATTAT 
GTTTCTGCAC CAAGTAATCC CTTGAATATG TCTGATTCAA CAAAACATAA TGAGCTTAGG 
GGATGCCAAA AGCAGCACCA TGTGCATGGC TCAGATGAGC TTTCTGGTAG TTATCCACCC 
AGTAAGCAGC CCGAGCACAG GAGAAGTGCT GGCCATCTTC ATCATAAGGA ATACTCGCAA 
CAACCCTTTC CAACTAAAAG CGAATCAAAG ATATGTCCCC AGAAAACACC TATGTTGTAT 
TCTCTGGCCC AAGGGCACAA TGACACAGAT GACTGTTCTA ATCAGATCAA AAGTGGAAGC 
GCACAACATG AAGCCCTTGA CAGTCTCAGT AGTAAACAGG TTAGACGGAA TGACCGATTT 
GCCACCACTT TGCGCAACGA GATCCAGATG AGAAGGGCGC ATCTCCAAAA GAGTCAAAGT 
GCAGCCACTT TAGAAAGTCC AGTTGAAACT GTAGAAGAGT CTGCTTTGTG GAAGACCACA 
GGGACTGCTT CTCCCTCATC AGATGGCTGC TTTTCCATTT CTTATAAAGA CCATCTTAAA 
GAAGCTCAGG CCAGAGTCCT CCAGGCTACA TCATTCAGGA GAAAAGACCT AGAGCCAGTA 
TTATTTGAGC ATCCAGGTAC TGAAGGTCCC ACACGAAAAG ATGTACCTCC TTCAAGTGTC 
TCAGAGGTCA CACCTAGCAA ACCCACCTCA GGGAGTAATC ATGTGCTTCG CATTGGAGGC 
CGTAAGCGGA TATCTGCTGA AAAGAAAGTG ACGTCTTTTT CTGAGCCAGA TAAAATTCAT 
GAAGTAGGAG CTGATGAACG CTCCACTATG CCTGAAAATG TTGCACACTT AGAAAATCAA 
CAAAGATTCT TTGAGACAAC AGGGAAACCA GTTTTCCCTA AGCCTATGCC AAAGCAAAAC 
CTGCAGATAT CAGAAGACCC CAGACTGTCC AAGCCTGGAG GCATGCACTA TTCTGCAAAC 
AGTGATATGG CAGGGCGGAA TAATGGTGAA AGCTCAACCC CCATTGAACA CCACATTAAT 
GAAGGTCTGG ATGGCCCTAA CTCAGTGAAC CATCAAGCAT TGCTAGAACA ACAGCGACTA 
GGCACATTTG CAGAGTATGA GGCCAAATGG AACACCCAGA GGAAGACTAC AGAACCAAGA 
GTATCTGGCC GATACCACTC TGCTGATAAC ATCCTCGATA CAGGAAAAGA GCGACGGAGT 
AACCCCACCT GTGTGCATGA AAGATCTAGA TCCTCACCTT CTGCTGACTT TTATGGGCAG 
AAACTTCCAC CACAAGAAAA GAAGTCAGCT GACTGTTTCA AATCTGAAAA GAGTCCTTGC 
CAACAGGACA AGAATAGTAC AAGTCAAAAT TTCTACCCAT CTGTTTCTTT GGAGCACAGC 
CACTGGTTCT ATACACCCAG CAGGTTAAAT GAAAAAGGAT ATAGTGAAAT CTTATGCAAG 
GAGAAACCAG AAACACCTCC TGAAGAACCT GAGAAAAAGA CTGTAGATCC TCCCTCCAGC 
CATCATAAGC CTGCACTACA TTTCCCTGTC CATAGCACTC GCAGTGAACC ATCAGCCCTC 
TCCAAAAACA AGAGCTCATT TCTCTTGCCC CATCTGGAGA AGTACAAATG CCCTGAGGGA 
ACACCTCCTC CTCTTAGCAA TATTCAGGAG GTCCAGCCTG GATCACAAGG AGGAGCTCTG 
GCCCCAATTT CCCCAGTCAA CAATCAAAGC TCCGCCCCAG TTTCAGCCCC CATTCCTTGG 
AAGAGTCCAG AGTACAGCAA AGGGCCTGTC GGGGAGGAGG AGCAACACCA AGAGCTTGTG 
CCTCCTCCCT TTCCACCTCC TCCCCCTCCC GTTGTCCTGC CCACCCAACA AACCGCTGGC 
CCGACCCAGC CCACAATGGA TGGGCAGCGT TCGCCCTCGC CCCAGTTCGC TCCCCAGAGG 
CTGACTGACA AGCCCCCTGT CTCCGTCTCC TCACAGGATG AAGCTCCTGG AAGGATGGAT 
CAGATTAAAG ATGAGAACAC ATCTGTGAAG AAAGTGCCCA TTAAGATAGT CCATTCAGAG 
AGTGACACGG AAAAAGAAAG TCGGCAATAT CTAGACCTGC CCAGCGAGAC GCCTGTCAAC 
TCGCAGGGAC TTGTGGGAGC TCATCTTCAA AGTTTGGGGA ACCCGGATCA GTCATACTCT 
TTGTTTTGTA CATACACCAG ACAAAAAGAT CAGGTTCCTG GTCTTAGAGA CCCAGACATG 
GGCCCTCTAA AAGACCAGGG GCCACAAAGC AATATAAACC CAGTGTCTTA TGTGGAACCT 
GGCCTTCAGA CAGGTCCACC ATTGGATCAG AGTACCAATG GAGTGTCTTT GCATCCTTCA 
CATTCAGAGG ATGATAAAAG AAAGGAGCTG GCTAGAGACA TAATGGACAA AGACAAGTCC 
CTCGCTGACA TTTTAGACCA GAGTAAGATG AAGACCACTA TGGATCTGAT GGAGGGGATT 
TTCCCTCAAG GAGATCAGCT ACTGGAGGAG GCCCAACAGC GTAGGAAGGC TCAACCTAAA 
CCACTCTCCC CTCGAAACTC TGTTGAGAAG AAAGAGGAAG ACAGCCTGGT GGCGGCCGCT 
GCTTTAGTGA GCAACTCAAC CTACTACAGC ACATCTGCAC CCAAAGCAGA GCTGCTGATC 
AAGATGAAGG ACATGCAGGA CCAGAGCGTT GAACACAACT CAGAAGAGGA GCTGGAGGAG 
GACAATGAAA GTGACCTCGC CTGCAAGAAG CACGAGTTGA TTGAAAGTCT CAGTAAGAAG 
TTGCAGGTCT TAAAAGAAGC TCAGGAGAGC CTGCAGGAGG ACGTGCAGGA CAACAACGCT 
CTTGGAGAAG AAGTGGAGGC CATCGTACAG GGCGTCTGCA AGCCTAATGA ACTCGACAAA 
TTCCGCATGT TCGTCGGGGA TCTTGATAAA GTGGTCAACC TTCTGCTGTC TCTGTCAGGA 
CGTTTGGCCA GGGTAGAGAA TGCCCTGAAC AGCCTGGAGG AAGATGCTTC GCTGGAGGAA 
AGGCGCACTC TGACAGAGAA ACGCAAACTG CTGATTCGTC AGCACGAGGA TGCTAAGGAG 
CTGAAGGAGA ACCTGGACAG GAGAGAGCGT GTGGTCTACG ACATTCTGGC CAGTTACCTG 
CCTGAGGAGA GTATGGCTGA CTACGAGCAC TTTGTCAAGA TGAAATCCGC TCTTATTATC 
GAGCAACGCA AGCTGGAGGA CAAGATCAAA CTGGGAGATG AGCAGCTCAA GTGTCTGATG 
GACAGTCTCC CAATGGACCA GAGAATAATC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212122.1
CDS	772..5907
Translation	MDTINQYPVNPSFPEDDHRSFCSSIRSASFNEHHVNDEGWKVVEVTLSGGAPWGFTLRGGLEHGEPLLITKVEEGSKAATVRLQVGDELININNISLTGFRQEAICLVKSSYKTLSLVVKRKMKMVDIVAQKMPSESDVHMARSFLTKILRSSMRRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356633.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356633.2

ORF Insert Sequence:

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAAAATGA AAATGGTGGA TATTGTAGCG CAGAAAATGC CATCAGAGAG CGACGTGCAT 
ATGGCCCGGA GCTTTCTCAC GAAGATTTTA CGAAGTTCCA TGAGGAGAAA TGAACCCATG 
AGCAGACCTC ACTCCTGGCA TGCCACCAAG TTCAACGAGA CCCATTCAGA AGCCAAAACC 
CAGTCCACAC CCTCACCAGT TTGGCAAACA AGATATGATG CAAGTTCATT CTCTTCTGAA 
CTCTCAACTG GCTGGGATCA AACAAATCTA CGGAGAGTAT CCGATCAGTT CAGTTCTTTG 
GGGAGTATGG ACAGCCTAGA ACATAGTTCT CATCCCTATC CACCTGGCCG TCTTTCCCCA 
AACAAATCCA ACAATAATAG CATTGAACAT TTGGGTAGTG GTAAACGGGA CTCGGCATAC 
AGCTCTTTTT CTACAAGTTC GGGAACCCCA GATTACACAC TGTCCAAAAG TAACACGGCA 
TCCACTGAGA ACATGCTGTA CAAAATAAAT CAGTGGGATT CAAGTGCCAG GCACAGCAAT 
GGCAGGCACA GCCAAAACCT AAGCGAAGGG GTACGACAAG ATGAGAGGCC TGGATACCTG 
CACCACCTCT CAAGTACAGG TAACCACGAG ATCCCAAGAG CTGAAGAACT GCCTGGCAAC 
CGTCATTCAA GCTCTGGAAG AGTTAATATT GGACCTGTCT GGCATGTTCC AGATATGAAG 
AAAAACATGA TATCATCTAC TCCACCTCCC ACTCCACCCA CACGCAGTGA TAGCTTTGCA 
GCTACCAAGG TTCACGAGAA GGGTCTGATC ACAGGTACTT CAGAGGGCCT TGGTGGTCAT 
TCTCAAGTAA AGCCTCAAGC GAAACCACAA CAAAAGCCAG GAGAAACCCA TGAAAGCACA 
CAAAGGTCTA ACCAAGTCAT CGAGATTTGT CTTGAGGGTC GACGGGATTA TAATCTACAA 
TCCAAAAATG ATTCCTCAAA TAGCTGCATT TCATCTGATT CTCATCATCA GTATATCCAA 
CGTACATCAT CAGATAAGAC ATACTCCCTA TCAACCACAG ACATAAGAGA TAGCCATCCA 
TCCTATGGCC ATGCTCCATA CCATGCACAG CAGTACAGTG ACGAAGGCAC TTTCCATGCT 
CAAACTAGAA CAGCAATGAA ACCTCCATTC AGTGGCTATT TCAGTAGTAT GCAGGAACTG 
CACACAAACA ACCACATGCA ACTCAACAGC CAGAATCAAA CTGGAAGACC AGCTGCATCA 
CTGTCAGTTA ATTTAGGAGT CAATTCCCAC CATATCTCTC AGGGATTAAC ACAGGCTTCT 
TTAATGAGAG CTGATGACTC TAAAGCCTCT TCAGTCTCAG AAATGTCCCA CAATGGACGG 
GACAGAATGT CCATAGGTTC TCAAGGAGGG AATAAAGAGC GGTATTTCCC TCCTCAATCG 
CAGCACCATG ATAATAAAGA AAGAAGTGCC TCCTTTAAAC AAAATGAAAA CCAACATTAT 
GTTTCTGCAC CAAGTAATCC CTTGAATATG TCTGATTCAA CAAAACATAA TGAGCTTAGG 
GGATGCCAAA AGCAGCACCA TGTGCATGGC TCAGATGAGC TTTCTGGTAG TTATCCACCC 
AGTAAGCAGC CCGAGCACAG GAGAAGTGCT GGCCATCTTC ATCATAAGGA ATACTCGCAA 
CAACCCTTTC CAACTAAAAG CGAATCAAAG ATATGTCCCC AGAAAACACC TATGTTGTAT 
TCTCTGGCCC AAGGGCACAA TGACACAGAT GACTGTTCTA ATCAGATCAA AAGTGGAAGC 
GCACAACATG AAGCCCTTGA CAGTCTCAGT AGTAAACAGG TTAGACGGAA TGACCGATTT 
GCCACCACTT TGCGCAACGA GATCCAGATG AGAAGGGCGC ATCTCCAAAA GAGTCAAAGT 
GCAGCCACTT TAGAAAGTCC AGTTGAAACT GTAGAAGAGT CTGCTTTGTG GAAGACCACA 
GGGACTGCTT CTCCCTCATC AGATGGCTGC TTTTCCATTT CTTATAAAGA CCATCTTAAA 
GAAGCTCAGG CCAGAGTCCT CCAGGCTACA TCATTCAGGA GAAAAGACCT AGAGCCAGTA 
TTATTTGAGC ATCCAGGTAC TGAAGGTCCC ACACGAAAAG ATGTACCTCC TTCAAGTGTC 
TCAGAGGTCA CACCTAGCAA ACCCACCTCA GGGAGTAATC ATGTGCTTCG CATTGGAGGC 
CGTAAGCGGA TATCTGCTGA AAAGAAAGTG ACGTCTTTTT CTGAGCCAGA TAAAATTCAT 
GAAGTAGGAG CTGATGAACG CTCCACTATG CCTGAAAATG TTGCACACTT AGAAAATCAA 
CAAAGATTCT TTGAGACAAC AGGGAAACCA GTTTTCCCTA AGCCTATGCC AAAGCAAAAC 
CTGCAGATAT CAGAAGACCC CAGACTGTCC AAGCCTGGAG GCATGCACTA TTCTGCAAAC 
AGTGATATGG CAGGGCGGAA TAATGGTGAA AGCTCAACCC CCATTGAACA CCACATTAAT 
GAAGGTCTGG ATGGCCCTAA CTCAGTGAAC CATCAAGCAT TGCTAGAACA ACAGCGACTA 
GGCACATTTG CAGAGTATGA GGCCAAATGG AACACCCAGA GGAAGACTAC AGAACCAAGA 
GTATCTGGCC GATACCACTC TGCTGATAAC ATCCTCGATA CAGGAAAAGA GCGACGGAGT 
AACCCCACCT GTGTGCATGA AAGATCTAGA TCCTCACCTT CTGCTGACTT TTATGGGCAG 
AAACTTCCAC CACAAGAAAA GAAGTCAGCT GACTGTTTCA AATCTGAAAA GAGTCCTTGC 
CAACAGGACA AGAATAGTAC AAGTCAAAAT TTCTACCCAT CTGTTTCTTT GGAGCACAGC 
CACTGGTTCT ATACACCCAG CAGGTTAAAT GAAAAAGGAT ATAGTGAAAT CTTATGCAAG 
GAGAAACCAG AAACACCTCC TGAAGAACCT GAGAAAAAGA CTGTAGATCC TCCCTCCAGC 
CATCATAAGC CTGCACTACA TTTCCCTGTC CATAGCACTC GCAGTGAACC ATCAGCCCTC 
TCCAAAAACA AGAGCTCATT TCTCTTGCCC CATCTGGAGA AGTACAAATG CCCTGAGGGA 
ACACCTCCTC CTCTTAGCAA TATTCAGGAG GTCCAGCCTG GATCACAAGG AGGAGCTCTG 
GCCCCAATTT CCCCAGTCAA CAATCAAAGC TCCGCCCCAG TTTCAGCCCC CATTCCTTGG 
AAGAGTCCAG AGTACAGCAA AGGGCCTGTC GGGGAGGAGG AGCAACACCA AGAGCTTGTG 
CCTCCTCCCT TTCCACCTCC TCCCCCTCCC GTTGTCCTGC CCACCCAACA AACCGCTGGC 
CCGACCCAGC CCACAATGGA TGGGCAGCGT TCGCCCTCGC CCCAGTTCGC TCCCCAGAGG 
CTGACTGACA AGCCCCCTGT CTCCGTCTCC TCACAGGATG AAGCTCCTGG AAGGATGGAT 
CAGATTAAAG ATGAGAACAC ATCTGTGAAG AAAGTGCCCA TTAAGATAGT CCATTCAGAG 
AGTGACACGG AAAAAGAAAG TCGGCAATAT CTAGACCTGC CCAGCGAGAC GCCTGTCAAC 
TCGCAGGGAC TTGTGGGAGC TCATCTTCAA AGTTTGGGGA ACCCGGATCA GTCATACTCT 
TTGTTTTGTA CATACACCAG ACAAAAAGAT CAGGTTCCTG GTCTTAGAGA CCCAGACATG 
GGCCCTCTAA AAGACCAGGG GCCACAAAGC AATATAAACC CAGTGTCTTA TGTGGAACCT 
GGCCTTCAGA CAGGTCCACC ATTGGATCAG AGTACCAATG GAGTGTCTTT GCATCCTTCA 
CATTCAGAGG ATGATAAAAG AAAGGAGCTG GCTAGAGACA TAATGGACAA AGACAAGTCC 
CTCGCTGACA TTTTAGACCA GAGTAAGATG AAGACCACTA TGGATCTGAT GGAGGGGATT 
TTCCCTCAAG GAGATCAGCT ACTGGAGGAG GCCCAACAGC GTAGGAAGGC TCAACCTAAA 
CCACTCTCCC CTCGAAACTC TGTTGAGAAG AAAGAGGAAG ACAGCCTGGT GGCGGCCGCT 
GCTTTAGTGA GCAACTCAAC CTACTACAGC ACATCTGCAC CCAAAGCAGA GCTGCTGATC 
AAGATGAAGG ACATGCAGGA CCAGAGCGTT GAACACAACT CAGAAGAGGA GCTGGAGGAG 
GACAATGAAA GTGACCTCGC CTGCAAGAAG CACGAGTTGA TTGAAAGTCT CAGTAAGAAG 
TTGCAGGTCT TAAAAGAAGC TCAGGAGAGC CTGCAGGAGG ACGTGCAGGA CAACAACGCT 
CTTGGAGAAG AAGTGGAGGC CATCGTACAG GGCGTCTGCA AGCCTAATGA ACTCGACAAA 
TTCCGCATGT TCGTCGGGGA TCTTGATAAA GTGGTCAACC TTCTGCTGTC TCTGTCAGGA 
CGTTTGGCCA GGGTAGAGAA TGCCCTGAAC AGCCTGGAGG AAGATGCTTC GCTGGAGGAA 
AGGCGCACTC TGACAGAGAA ACGCAAACTG CTGATTCGTC AGCACGAGGA TGCTAAGGAG 
CTGAAGGAGA ACCTGGACAG GAGAGAGCGT GTGGTCTACG ACATTCTGGC CAGTTACCTG 
CCTGAGGAGA GTATGGCTGA CTACGAGCAC TTTGTCAAGA TGAAATCCGC TCTTATTATC 
GAGCAACGCA AGCTGGAGGA CAAGATCAAA CTGGGAGATG AGCAGCTCAA GTGTCTGATG 
GACAGTCTCC CAATGGACCA GAGAATAATC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356634.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212123.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X3, mRNA.

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CloneID	ODa59473
Clone ID Related Accession (Same CDS sequence)	XM_017356634.2 , XM_017356634.1
Accession Version	XM_017356634.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356634.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAAAATGA AAATGGTGGA TATTGTAGCG CAGAAAATGC CATCAGAGAG CGACGTGCAT 
ATGGCCCGGA GCTTTCTCAC GAAGATTTTA CGAAGTTCCA TGAGAAAGAA TCGGTTTAAA 
GGGAGAAATG AACCCATGAG CAGACCTCAC TCCTGGCATG CCACCAAGTT CAACGAGACC 
CATTCAGAAG CCAAAACCCA GTCCACACCC TCACCAGTTT GGCAAACAAG ATATGATGCA 
AGTTCATTCT CTTCTGAACT CTCAACTGGC TGGGATCAAA CAAATCTACG GAGAGTATCC 
GATCAGTTCA GTTCTTTGGG GAGTATGGAC AGCCTAGAAC ATAGTTCTCA TCCCTATCCA 
CCTGGCCGTC TTTCCCCAAA CAAATCCAAC AATAATAGCA TTGAACATTT GGGTAGTGGT 
AAACGGGACT CGGCATACAG CTCTTTTTCT ACAAGTTCGG GAACCCCAGA TTACACACTG 
TCCAAAAGTA ACACGGCATC CACTGAGAAC ATGCTGTACA AAATAAATCA GTGGGATTCA 
AGTGCCAGGC ACAGCAATGG CAGGCACAGC CAAAACCTAA GCGAAGGGGT ACGACAAGAT 
GAGAGGCCTG GATACCTGCA CCACCTCTCA AGTACAGGTA ACCACGAGAT CCCAAGAGCT 
GAAGAACTGC CTGGCAACCG TCATTCAAGC TCTGGAAGAG TTAATATTGG ACCTGTCTGG 
CATGTTCCAG ATATGAAGAA AAACATGATA TCATCTACTC CACCTCCCAC TCCACCCACA 
CGCAGTGATA GCTTTGCAGC TACCAAGGTT CACGAGAAGG GTCTGATCAC AGGTACTTCA 
GAGGGCCTTG GTGGTCATTC TCAAGTAAAG CCTCAAGCGA AACCACAACA AAAGCCAGGA 
GAAACCCATG AAAGCACACA AAGGTCTAAC CAAGTCATCG AGATTTGTCT TGAGGGTCGA 
CGGGATTATA ATCTACAATC CAAAAATGAT TCCTCAAATA GCTGCATTTC ATCTGATTCT 
CATCATCAGT ATATCCAACG TACATCATCA GATAAGACAT ACTCCCTATC AACCACAGAC 
ATAAGAGATA GCCATCCATC CTATGGCCAT GCTCCATACC ATGCACAGCA GTACAGTGAC 
GAAGGCACTT TCCATGCTCA AACTAGAACA GCAATGAAAC CTCCATTCAG TGGCTATTTC 
AGTAGTATGC AGGAACTGCA CACAAACAAC CACATGCAAC TCAACAGCCA GAATCAAACT 
GGAAGACCAG CTGCATCACT GTCAGTTAAT TTAGGAGTCA ATTCCCACCA TATCTCTCAG 
GGATTAACAC AGGCTTCTTT AATGAGAGCT GATGACTCTA AAGCCTCTTC AGTCTCAGAA 
ATGTCCCACA ATGGACGGGA CAGAATGTCC ATAGGTTCTC AAGGAGGGAA TAAAGAGCGG 
TATTTCCCTC CTCAATCGCA GCACCATGAT AATAAAGAAA GAAGTGCCTC CTTTAAACAA 
AATGAAAACC AACATTATGT TTCTGCACCA AGTAATCCCT TGAATATGTC TGATTCAACA 
AAACATAATG AGCTTAGGGG ATGCCAAAAG CAGCACCATG TGCATGGCTC AGATGAGCTT 
TCTGGTAGTT ATCCACCCAG TAAGCAGCCC GAGCACAGGA GAAGTGCTGG CCATCTTCAT 
CATAAGGAAT ACTCGCAACA ACCCTTTCCA ACTAAAAGCG AATCAAAGAT ATGTCCCCAG 
AAAACACCTA TGTTGTATTC TCTGGCCCAA GGGCACAATG ACACAGATGA CTGTTCTAAT 
CAGATCAAAA GTGGAAGCGC ACAACATGAA GCCCTTGACA GTCTCAGTAG TAAACAGGTT 
AGACGGAATG ACCGATTTGC CACCACTTTG CGCAACGAGA TCCAGATGAG AAGGGCGCAT 
CTCCAAAAGA GTCAAAGTGC AGCCACTTTA GAAAGTCCAG TTGAAACTGT AGAAGAGTCT 
GCTTTGTGGA AGACCACAGG GACTGCTTCT CCCTCATCAG ATGGCTGCTT TTCCATTTCT 
TATAAAGACC ATCTTAAAGA AGCTCAGGCC AGAGTCCTCC AGGCTACATC ATTCAGGAGA 
AAAGACCTAG AGCCAGTATT ATTTGAGCAT CCAGGTACTG AAGGTCCCAC ACGAAAAGAT 
GTACCTCCTT CAAGTGTCTC AGAGGTCACA CCTAGCAAAC CCACCTCAGG GAGTAATCAT 
GTGCTTCGCA TTGGAGGCCG TAAGCGGATA TCTGCTGAAA AGAAAGTGAC GTCTTTTTCT 
GAGCCAGATA AAATTCATGA AGTAGGAGCT GATGAACGCT CCACTATGCC TGAAAATGTT 
GCACACTTAG AAAATCAACA AAGATTCTTT GAGACAACAG GGAAACCAGT TTTCCCTAAG 
CCTATGCCAA AGCAAAACCT GCAGATATCA GAAGACCCCA GACTGTCCAA GCCTGGAGGC 
ATGCACTATT CTGCAAACAG TGATATGGCA GGGCGGAATA ATGGTGAAAG CTCAACCCCC 
ATTGAACACC ACATTAATGA AGGTCTGGAT GGCCCTAACT CAGTGAACCA TCAAGCATTG 
CTAGAACAAC AGCGACTAGG CACATTTGCA GAGTATGAGG CCAAATGGAA CACCCAGAGG 
AAGACTACAG AACCAAGAGT ATCTGGCCGA TACCACTCTG CTGATAACAT CCTCGATACA 
GGAAAAGAGC GACGGAGTAA CCCCACCTGT GTGCATGAAA GATCTAGATC CTCACCTTCT 
GCTGACTTTT ATGGGCAGAA ACTTCCACCA CAAGAAAAGA AGTCAGCTGA CTGTTTCAAA 
TCTGAAAAGA GTCCTTGCCA ACAGGACAAG AATAGTACAA GGTTAAATGA AAAAGGATAT 
AGTGAAATCT TATGCAAGGA GAAACCAGAA ACACCTCCTG AAGAACCTGA GAAAAAGACT 
GTAGATCCTC CCTCCAGCCA TCATAAGCCT GCACTACATT TCCCTGTCCA TAGCACTCGC 
AGTGAACCAT CAGCCCTCTC CAAAAACAAG AGCTCATTTC TCTTGCCCCA TCTGGAGAAG 
TACAAATGCC CTGAGGGAAC ACCTCCTCCT CTTAGCAATA TTCAGGAGGT CCAGCCTGGA 
TCACAAGGAG GAGCTCTGGC CCCAATTTCC CCAGTCAACA ATCAAAGCTC CGCCCCAGTT 
TCAGCCCCCA TTCCTTGGAA GAGTCCAGAG TACAGCAAAG GGCCTGTCGG GGAGGAGGAG 
CAACACCAAG AGCTTGTGCC TCCTCCCTTT CCACCTCCTC CCCCTCCCGT TGTCCTGCCC 
ACCCAACAAA CCGCTGGCCC GACCCAGCCC ACAATGGATG GGCAGCGTTC GCCCTCGCCC 
CAGTTCGCTC CCCAGAGGCT GACTGACAAG CCCCCTGTCT CCGTCTCCTC ACAGGATGAA 
GCTCCTGGAA GGATGGATCA GATTAAAGAT GAGAACACAT CTGTGAAGAA AGTGCCCATT 
AAGATAGTCC ATTCAGAGAG TGACACGGAA AAAGAAAGTC GGCAATATCT AGACCTGCCC 
AGCGAGACGC CTGTCAACTC GCAGGGACTT GTGGGAGCTC ATCTTCAAAG TTTGGGGAAC 
CCGGATCAGT CATACTCTTT GTTTTGTACA TACACCAGAC AAAAAGATCA GGTTCCTGGT 
CTTAGAGACC CAGACATGGG CCCTCTAAAA GACCAGGGGC CACAAAGCAA TATAAACCCA 
GTGTCTTATG TGGAACCTGG CCTTCAGACA GGTCCACCAT TGGATCAGAG TACCAATGGA 
GTGTCTTTGC ATCCTTCACA TTCAGAGGAT GATAAAAGAA AGGAGCTGGC TAGAGACATA 
ATGGACAAAG ACAAGTCCCT CGCTGACATT TTAGACCAGA GTAAGATGAA GACCACTATG 
GATCTGATGG AGGGGATTTT CCCTCAAGGA GATCAGCTAC TGGAGGAGGC CCAACAGCGT 
AGGAAGGCTC AACCTAAACC ACTCTCCCCT CGAAACTCTG TTGAGAAGAA AGAGGAAGAC 
AGCCTGGTGG CGGCCGCTGC TTTAGTGAGC AACTCAACCT ACTACAGCAC ATCTGCACCC 
AAAGCAGAGC TGCTGATCAA GATGAAGGAC ATGCAGGACC AGAGCGTTGA ACACAACTCA 
GAAGAGGAGC TGGAGGAGGA CAATGAAAGT GACCTCGCCT GCAAGAAGCA CGAGTTGATT 
GAAAGTCTCA GTAAGAAGTT GCAGGTCTTA AAAGAAGCTC AGGAGAGCCT GCAGGAGGAC 
GTGCAGGACA ACAACGCTCT TGGAGAAGAA GTGGAGGCCA TCGTACAGGG CGTCTGCAAG 
CCTAATGAAC TCGACAAATT CCGCATGTTC GTCGGGGATC TTGATAAAGT GGTCAACCTT 
CTGCTGTCTC TGTCAGGACG TTTGGCCAGG GTAGAGAATG CCCTGAACAG CCTGGAGGAA 
GATGCTTCGC TGGAGGAAAG GCGCACTCTG ACAGAGAAAC GCAAACTGCT GATTCGTCAG 
CACGAGGATG CTAAGGAGCT GAAGGAGAAC CTGGACAGGA GAGAGCGTGT GGTCTACGAC 
ATTCTGGCCA GTTACCTGCC TGAGGAGAGT ATGGCTGACT ACGAGCACTT TGTCAAGATG 
AAATCCGCTC TTATTATCGA GCAACGCAAG CTGGAGGACA AGATCAAACT GGGAGATGAG 
CAGCTCAAGT GTCTGATGGA CAGTCTCCCA ATGGACCAGA GAATAATCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212123.1
CDS	772..5865
Translation	MDTINQYPVNPSFPEDDHRSFCSSIRSASFNEHHVNDEGWKVVEVTLSGGAPWGFTLRGGLEHGEPLLITKVEEGSKAATVRLQVGDELININNISLTGFRQEAICLVKSSYKTLSLVVKRKMKMVDIVAQKMPSESDVHMARSFLTKILRSSMRKNRFKGRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356634.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356634.2

ORF Insert Sequence:

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAAAATGA AAATGGTGGA TATTGTAGCG CAGAAAATGC CATCAGAGAG CGACGTGCAT 
ATGGCCCGGA GCTTTCTCAC GAAGATTTTA CGAAGTTCCA TGAGAAAGAA TCGGTTTAAA 
GGGAGAAATG AACCCATGAG CAGACCTCAC TCCTGGCATG CCACCAAGTT CAACGAGACC 
CATTCAGAAG CCAAAACCCA GTCCACACCC TCACCAGTTT GGCAAACAAG ATATGATGCA 
AGTTCATTCT CTTCTGAACT CTCAACTGGC TGGGATCAAA CAAATCTACG GAGAGTATCC 
GATCAGTTCA GTTCTTTGGG GAGTATGGAC AGCCTAGAAC ATAGTTCTCA TCCCTATCCA 
CCTGGCCGTC TTTCCCCAAA CAAATCCAAC AATAATAGCA TTGAACATTT GGGTAGTGGT 
AAACGGGACT CGGCATACAG CTCTTTTTCT ACAAGTTCGG GAACCCCAGA TTACACACTG 
TCCAAAAGTA ACACGGCATC CACTGAGAAC ATGCTGTACA AAATAAATCA GTGGGATTCA 
AGTGCCAGGC ACAGCAATGG CAGGCACAGC CAAAACCTAA GCGAAGGGGT ACGACAAGAT 
GAGAGGCCTG GATACCTGCA CCACCTCTCA AGTACAGGTA ACCACGAGAT CCCAAGAGCT 
GAAGAACTGC CTGGCAACCG TCATTCAAGC TCTGGAAGAG TTAATATTGG ACCTGTCTGG 
CATGTTCCAG ATATGAAGAA AAACATGATA TCATCTACTC CACCTCCCAC TCCACCCACA 
CGCAGTGATA GCTTTGCAGC TACCAAGGTT CACGAGAAGG GTCTGATCAC AGGTACTTCA 
GAGGGCCTTG GTGGTCATTC TCAAGTAAAG CCTCAAGCGA AACCACAACA AAAGCCAGGA 
GAAACCCATG AAAGCACACA AAGGTCTAAC CAAGTCATCG AGATTTGTCT TGAGGGTCGA 
CGGGATTATA ATCTACAATC CAAAAATGAT TCCTCAAATA GCTGCATTTC ATCTGATTCT 
CATCATCAGT ATATCCAACG TACATCATCA GATAAGACAT ACTCCCTATC AACCACAGAC 
ATAAGAGATA GCCATCCATC CTATGGCCAT GCTCCATACC ATGCACAGCA GTACAGTGAC 
GAAGGCACTT TCCATGCTCA AACTAGAACA GCAATGAAAC CTCCATTCAG TGGCTATTTC 
AGTAGTATGC AGGAACTGCA CACAAACAAC CACATGCAAC TCAACAGCCA GAATCAAACT 
GGAAGACCAG CTGCATCACT GTCAGTTAAT TTAGGAGTCA ATTCCCACCA TATCTCTCAG 
GGATTAACAC AGGCTTCTTT AATGAGAGCT GATGACTCTA AAGCCTCTTC AGTCTCAGAA 
ATGTCCCACA ATGGACGGGA CAGAATGTCC ATAGGTTCTC AAGGAGGGAA TAAAGAGCGG 
TATTTCCCTC CTCAATCGCA GCACCATGAT AATAAAGAAA GAAGTGCCTC CTTTAAACAA 
AATGAAAACC AACATTATGT TTCTGCACCA AGTAATCCCT TGAATATGTC TGATTCAACA 
AAACATAATG AGCTTAGGGG ATGCCAAAAG CAGCACCATG TGCATGGCTC AGATGAGCTT 
TCTGGTAGTT ATCCACCCAG TAAGCAGCCC GAGCACAGGA GAAGTGCTGG CCATCTTCAT 
CATAAGGAAT ACTCGCAACA ACCCTTTCCA ACTAAAAGCG AATCAAAGAT ATGTCCCCAG 
AAAACACCTA TGTTGTATTC TCTGGCCCAA GGGCACAATG ACACAGATGA CTGTTCTAAT 
CAGATCAAAA GTGGAAGCGC ACAACATGAA GCCCTTGACA GTCTCAGTAG TAAACAGGTT 
AGACGGAATG ACCGATTTGC CACCACTTTG CGCAACGAGA TCCAGATGAG AAGGGCGCAT 
CTCCAAAAGA GTCAAAGTGC AGCCACTTTA GAAAGTCCAG TTGAAACTGT AGAAGAGTCT 
GCTTTGTGGA AGACCACAGG GACTGCTTCT CCCTCATCAG ATGGCTGCTT TTCCATTTCT 
TATAAAGACC ATCTTAAAGA AGCTCAGGCC AGAGTCCTCC AGGCTACATC ATTCAGGAGA 
AAAGACCTAG AGCCAGTATT ATTTGAGCAT CCAGGTACTG AAGGTCCCAC ACGAAAAGAT 
GTACCTCCTT CAAGTGTCTC AGAGGTCACA CCTAGCAAAC CCACCTCAGG GAGTAATCAT 
GTGCTTCGCA TTGGAGGCCG TAAGCGGATA TCTGCTGAAA AGAAAGTGAC GTCTTTTTCT 
GAGCCAGATA AAATTCATGA AGTAGGAGCT GATGAACGCT CCACTATGCC TGAAAATGTT 
GCACACTTAG AAAATCAACA AAGATTCTTT GAGACAACAG GGAAACCAGT TTTCCCTAAG 
CCTATGCCAA AGCAAAACCT GCAGATATCA GAAGACCCCA GACTGTCCAA GCCTGGAGGC 
ATGCACTATT CTGCAAACAG TGATATGGCA GGGCGGAATA ATGGTGAAAG CTCAACCCCC 
ATTGAACACC ACATTAATGA AGGTCTGGAT GGCCCTAACT CAGTGAACCA TCAAGCATTG 
CTAGAACAAC AGCGACTAGG CACATTTGCA GAGTATGAGG CCAAATGGAA CACCCAGAGG 
AAGACTACAG AACCAAGAGT ATCTGGCCGA TACCACTCTG CTGATAACAT CCTCGATACA 
GGAAAAGAGC GACGGAGTAA CCCCACCTGT GTGCATGAAA GATCTAGATC CTCACCTTCT 
GCTGACTTTT ATGGGCAGAA ACTTCCACCA CAAGAAAAGA AGTCAGCTGA CTGTTTCAAA 
TCTGAAAAGA GTCCTTGCCA ACAGGACAAG AATAGTACAA GGTTAAATGA AAAAGGATAT 
AGTGAAATCT TATGCAAGGA GAAACCAGAA ACACCTCCTG AAGAACCTGA GAAAAAGACT 
GTAGATCCTC CCTCCAGCCA TCATAAGCCT GCACTACATT TCCCTGTCCA TAGCACTCGC 
AGTGAACCAT CAGCCCTCTC CAAAAACAAG AGCTCATTTC TCTTGCCCCA TCTGGAGAAG 
TACAAATGCC CTGAGGGAAC ACCTCCTCCT CTTAGCAATA TTCAGGAGGT CCAGCCTGGA 
TCACAAGGAG GAGCTCTGGC CCCAATTTCC CCAGTCAACA ATCAAAGCTC CGCCCCAGTT 
TCAGCCCCCA TTCCTTGGAA GAGTCCAGAG TACAGCAAAG GGCCTGTCGG GGAGGAGGAG 
CAACACCAAG AGCTTGTGCC TCCTCCCTTT CCACCTCCTC CCCCTCCCGT TGTCCTGCCC 
ACCCAACAAA CCGCTGGCCC GACCCAGCCC ACAATGGATG GGCAGCGTTC GCCCTCGCCC 
CAGTTCGCTC CCCAGAGGCT GACTGACAAG CCCCCTGTCT CCGTCTCCTC ACAGGATGAA 
GCTCCTGGAA GGATGGATCA GATTAAAGAT GAGAACACAT CTGTGAAGAA AGTGCCCATT 
AAGATAGTCC ATTCAGAGAG TGACACGGAA AAAGAAAGTC GGCAATATCT AGACCTGCCC 
AGCGAGACGC CTGTCAACTC GCAGGGACTT GTGGGAGCTC ATCTTCAAAG TTTGGGGAAC 
CCGGATCAGT CATACTCTTT GTTTTGTACA TACACCAGAC AAAAAGATCA GGTTCCTGGT 
CTTAGAGACC CAGACATGGG CCCTCTAAAA GACCAGGGGC CACAAAGCAA TATAAACCCA 
GTGTCTTATG TGGAACCTGG CCTTCAGACA GGTCCACCAT TGGATCAGAG TACCAATGGA 
GTGTCTTTGC ATCCTTCACA TTCAGAGGAT GATAAAAGAA AGGAGCTGGC TAGAGACATA 
ATGGACAAAG ACAAGTCCCT CGCTGACATT TTAGACCAGA GTAAGATGAA GACCACTATG 
GATCTGATGG AGGGGATTTT CCCTCAAGGA GATCAGCTAC TGGAGGAGGC CCAACAGCGT 
AGGAAGGCTC AACCTAAACC ACTCTCCCCT CGAAACTCTG TTGAGAAGAA AGAGGAAGAC 
AGCCTGGTGG CGGCCGCTGC TTTAGTGAGC AACTCAACCT ACTACAGCAC ATCTGCACCC 
AAAGCAGAGC TGCTGATCAA GATGAAGGAC ATGCAGGACC AGAGCGTTGA ACACAACTCA 
GAAGAGGAGC TGGAGGAGGA CAATGAAAGT GACCTCGCCT GCAAGAAGCA CGAGTTGATT 
GAAAGTCTCA GTAAGAAGTT GCAGGTCTTA AAAGAAGCTC AGGAGAGCCT GCAGGAGGAC 
GTGCAGGACA ACAACGCTCT TGGAGAAGAA GTGGAGGCCA TCGTACAGGG CGTCTGCAAG 
CCTAATGAAC TCGACAAATT CCGCATGTTC GTCGGGGATC TTGATAAAGT GGTCAACCTT 
CTGCTGTCTC TGTCAGGACG TTTGGCCAGG GTAGAGAATG CCCTGAACAG CCTGGAGGAA 
GATGCTTCGC TGGAGGAAAG GCGCACTCTG ACAGAGAAAC GCAAACTGCT GATTCGTCAG 
CACGAGGATG CTAAGGAGCT GAAGGAGAAC CTGGACAGGA GAGAGCGTGT GGTCTACGAC 
ATTCTGGCCA GTTACCTGCC TGAGGAGAGT ATGGCTGACT ACGAGCACTT TGTCAAGATG 
AAATCCGCTC TTATTATCGA GCAACGCAAG CTGGAGGACA AGATCAAACT GGGAGATGAG 
CAGCTCAAGT GTCTGATGGA CAGTCTCCCA ATGGACCAGA GAATAATCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356634.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212123.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X3, mRNA.

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CloneID	ODa59473
Clone ID Related Accession (Same CDS sequence)	XM_017356634.2 , XM_017356634.1
Accession Version	XM_017356634.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAAAATGA AAATGGTGGA TATTGTAGCG CAGAAAATGC CATCAGAGAG CGACGTGCAT 
ATGGCCCGGA GCTTTCTCAC GAAGATTTTA CGAAGTTCCA TGAGAAAGAA TCGGTTTAAA 
GGGAGAAATG AACCCATGAG CAGACCTCAC TCCTGGCATG CCACCAAGTT CAACGAGACC 
CATTCAGAAG CCAAAACCCA GTCCACACCC TCACCAGTTT GGCAAACAAG ATATGATGCA 
AGTTCATTCT CTTCTGAACT CTCAACTGGC TGGGATCAAA CAAATCTACG GAGAGTATCC 
GATCAGTTCA GTTCTTTGGG GAGTATGGAC AGCCTAGAAC ATAGTTCTCA TCCCTATCCA 
CCTGGCCGTC TTTCCCCAAA CAAATCCAAC AATAATAGCA TTGAACATTT GGGTAGTGGT 
AAACGGGACT CGGCATACAG CTCTTTTTCT ACAAGTTCGG GAACCCCAGA TTACACACTG 
TCCAAAAGTA ACACGGCATC CACTGAGAAC ATGCTGTACA AAATAAATCA GTGGGATTCA 
AGTGCCAGGC ACAGCAATGG CAGGCACAGC CAAAACCTAA GCGAAGGGGT ACGACAAGAT 
GAGAGGCCTG GATACCTGCA CCACCTCTCA AGTACAGGTA ACCACGAGAT CCCAAGAGCT 
GAAGAACTGC CTGGCAACCG TCATTCAAGC TCTGGAAGAG TTAATATTGG ACCTGTCTGG 
CATGTTCCAG ATATGAAGAA AAACATGATA TCATCTACTC CACCTCCCAC TCCACCCACA 
CGCAGTGATA GCTTTGCAGC TACCAAGGTT CACGAGAAGG GTCTGATCAC AGGTACTTCA 
GAGGGCCTTG GTGGTCATTC TCAAGTAAAG CCTCAAGCGA AACCACAACA AAAGCCAGGA 
GAAACCCATG AAAGCACACA AAGGTCTAAC CAAGTCATCG AGATTTGTCT TGAGGGTCGA 
CGGGATTATA ATCTACAATC CAAAAATGAT TCCTCAAATA GCTGCATTTC ATCTGATTCT 
CATCATCAGT ATATCCAACG TACATCATCA GATAAGACAT ACTCCCTATC AACCACAGAC 
ATAAGAGATA GCCATCCATC CTATGGCCAT GCTCCATACC ATGCACAGCA GTACAGTGAC 
GAAGGCACTT TCCATGCTCA AACTAGAACA GCAATGAAAC CTCCATTCAG TGGCTATTTC 
AGTAGTATGC AGGAACTGCA CACAAACAAC CACATGCAAC TCAACAGCCA GAATCAAACT 
GGAAGACCAG CTGCATCACT GTCAGTTAAT TTAGGAGTCA ATTCCCACCA TATCTCTCAG 
GGATTAACAC AGGCTTCTTT AATGAGAGCT GATGACTCTA AAGCCTCTTC AGTCTCAGAA 
ATGTCCCACA ATGGACGGGA CAGAATGTCC ATAGGTTCTC AAGGAGGGAA TAAAGAGCGG 
TATTTCCCTC CTCAATCGCA GCACCATGAT AATAAAGAAA GAAGTGCCTC CTTTAAACAA 
AATGAAAACC AACATTATGT TTCTGCACCA AGTAATCCCT TGAATATGTC TGATTCAACA 
AAACATAATG AGCTTAGGGG ATGCCAAAAG CAGCACCATG TGCATGGCTC AGATGAGCTT 
TCTGGTAGTT ATCCACCCAG TAAGCAGCCC GAGCACAGGA GAAGTGCTGG CCATCTTCAT 
CATAAGGAAT ACTCGCAACA ACCCTTTCCA ACTAAAAGCG AATCAAAGAT ATGTCCCCAG 
AAAACACCTA TGTTGTATTC TCTGGCCCAA GGGCACAATG ACACAGATGA CTGTTCTAAT 
CAGATCAAAA GTGGAAGCGC ACAACATGAA GCCCTTGACA GTCTCAGTAG TAAACAGGTT 
AGACGGAATG ACCGATTTGC CACCACTTTG CGCAACGAGA TCCAGATGAG AAGGGCGCAT 
CTCCAAAAGA GTCAAAGTGC AGCCACTTTA GAAAGTCCAG TTGAAACTGT AGAAGAGTCT 
GCTTTGTGGA AGACCACAGG GACTGCTTCT CCCTCATCAG ATGGCTGCTT TTCCATTTCT 
TATAAAGACC ATCTTAAAGA AGCTCAGGCC AGAGTCCTCC AGGCTACATC ATTCAGGAGA 
AAAGACCTAG AGCCAGTATT ATTTGAGCAT CCAGGTACTG AAGGTCCCAC ACGAAAAGAT 
GTACCTCCTT CAAGTGTCTC AGAGGTCACA CCTAGCAAAC CCACCTCAGG GAGTAATCAT 
GTGCTTCGCA TTGGAGGCCG TAAGCGGATA TCTGCTGAAA AGAAAGTGAC GTCTTTTTCT 
GAGCCAGATA AAATTCATGA AGTAGGAGCT GATGAACGCT CCACTATGCC TGAAAATGTT 
GCACACTTAG AAAATCAACA AAGATTCTTT GAGACAACAG GGAAACCAGT TTTCCCTAAG 
CCTATGCCAA AGCAAAACCT GCAGATATCA GAAGACCCCA GACTGTCCAA GCCTGGAGGC 
ATGCACTATT CTGCAAACAG TGATATGGCA GGGCGGAATA ATGGTGAAAG CTCAACCCCC 
ATTGAACACC ACATTAATGA AGGTCTGGAT GGCCCTAACT CAGTGAACCA TCAAGCATTG 
CTAGAACAAC AGCGACTAGG CACATTTGCA GAGTATGAGG CCAAATGGAA CACCCAGAGG 
AAGACTACAG AACCAAGAGT ATCTGGCCGA TACCACTCTG CTGATAACAT CCTCGATACA 
GGAAAAGAGC GACGGAGTAA CCCCACCTGT GTGCATGAAA GATCTAGATC CTCACCTTCT 
GCTGACTTTT ATGGGCAGAA ACTTCCACCA CAAGAAAAGA AGTCAGCTGA CTGTTTCAAA 
TCTGAAAAGA GTCCTTGCCA ACAGGACAAG AATAGTACAA GGTTAAATGA AAAAGGATAT 
AGTGAAATCT TATGCAAGGA GAAACCAGAA ACACCTCCTG AAGAACCTGA GAAAAAGACT 
GTAGATCCTC CCTCCAGCCA TCATAAGCCT GCACTACATT TCCCTGTCCA TAGCACTCGC 
AGTGAACCAT CAGCCCTCTC CAAAAACAAG AGCTCATTTC TCTTGCCCCA TCTGGAGAAG 
TACAAATGCC CTGAGGGAAC ACCTCCTCCT CTTAGCAATA TTCAGGAGGT CCAGCCTGGA 
TCACAAGGAG GAGCTCTGGC CCCAATTTCC CCAGTCAACA ATCAAAGCTC CGCCCCAGTT 
TCAGCCCCCA TTCCTTGGAA GAGTCCAGAG TACAGCAAAG GGCCTGTCGG GGAGGAGGAG 
CAACACCAAG AGCTTGTGCC TCCTCCCTTT CCACCTCCTC CCCCTCCCGT TGTCCTGCCC 
ACCCAACAAA CCGCTGGCCC GACCCAGCCC ACAATGGATG GGCAGCGTTC GCCCTCGCCC 
CAGTTCGCTC CCCAGAGGCT GACTGACAAG CCCCCTGTCT CCGTCTCCTC ACAGGATGAA 
GCTCCTGGAA GGATGGATCA GATTAAAGAT GAGAACACAT CTGTGAAGAA AGTGCCCATT 
AAGATAGTCC ATTCAGAGAG TGACACGGAA AAAGAAAGTC GGCAATATCT AGACCTGCCC 
AGCGAGACGC CTGTCAACTC GCAGGGACTT GTGGGAGCTC ATCTTCAAAG TTTGGGGAAC 
CCGGATCAGT CATACTCTTT GTTTTGTACA TACACCAGAC AAAAAGATCA GGTTCCTGGT 
CTTAGAGACC CAGACATGGG CCCTCTAAAA GACCAGGGGC CACAAAGCAA TATAAACCCA 
GTGTCTTATG TGGAACCTGG CCTTCAGACA GGTCCACCAT TGGATCAGAG TACCAATGGA 
GTGTCTTTGC ATCCTTCACA TTCAGAGGAT GATAAAAGAA AGGAGCTGGC TAGAGACATA 
ATGGACAAAG ACAAGTCCCT CGCTGACATT TTAGACCAGA GTAAGATGAA GACCACTATG 
GATCTGATGG AGGGGATTTT CCCTCAAGGA GATCAGCTAC TGGAGGAGGC CCAACAGCGT 
AGGAAGGCTC AACCTAAACC ACTCTCCCCT CGAAACTCTG TTGAGAAGAA AGAGGAAGAC 
AGCCTGGTGG CGGCCGCTGC TTTAGTGAGC AACTCAACCT ACTACAGCAC ATCTGCACCC 
AAAGCAGAGC TGCTGATCAA GATGAAGGAC ATGCAGGACC AGAGCGTTGA ACACAACTCA 
GAAGAGGAGC TGGAGGAGGA CAATGAAAGT GACCTCGCCT GCAAGAAGCA CGAGTTGATT 
GAAAGTCTCA GTAAGAAGTT GCAGGTCTTA AAAGAAGCTC AGGAGAGCCT GCAGGAGGAC 
GTGCAGGACA ACAACGCTCT TGGAGAAGAA GTGGAGGCCA TCGTACAGGG CGTCTGCAAG 
CCTAATGAAC TCGACAAATT CCGCATGTTC GTCGGGGATC TTGATAAAGT GGTCAACCTT 
CTGCTGTCTC TGTCAGGACG TTTGGCCAGG GTAGAGAATG CCCTGAACAG CCTGGAGGAA 
GATGCTTCGC TGGAGGAAAG GCGCACTCTG ACAGAGAAAC GCAAACTGCT GATTCGTCAG 
CACGAGGATG CTAAGGAGCT GAAGGAGAAC CTGGACAGGA GAGAGCGTGT GGTCTACGAC 
ATTCTGGCCA GTTACCTGCC TGAGGAGAGT ATGGCTGACT ACGAGCACTT TGTCAAGATG 
AAATCCGCTC TTATTATCGA GCAACGCAAG CTGGAGGACA AGATCAAACT GGGAGATGAG 
CAGCTCAAGT GTCTGATGGA CAGTCTCCCA ATGGACCAGA GAATAATCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212123.1
CDS	766..5859
Translation	MDTINQYPVNPSFPEDDHRSFCSSIRSASFNEHHVNDEGWKVVEVTLSGGAPWGFTLRGGLEHGEPLLITKVEEGSKAATVRLQVGDELININNISLTGFRQEAICLVKSSYKTLSLVVKRKMKMVDIVAQKMPSESDVHMARSFLTKILRSSMRKNRFKGRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356634.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356634.1

ORF Insert Sequence:

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAAAATGA AAATGGTGGA TATTGTAGCG CAGAAAATGC CATCAGAGAG CGACGTGCAT 
ATGGCCCGGA GCTTTCTCAC GAAGATTTTA CGAAGTTCCA TGAGAAAGAA TCGGTTTAAA 
GGGAGAAATG AACCCATGAG CAGACCTCAC TCCTGGCATG CCACCAAGTT CAACGAGACC 
CATTCAGAAG CCAAAACCCA GTCCACACCC TCACCAGTTT GGCAAACAAG ATATGATGCA 
AGTTCATTCT CTTCTGAACT CTCAACTGGC TGGGATCAAA CAAATCTACG GAGAGTATCC 
GATCAGTTCA GTTCTTTGGG GAGTATGGAC AGCCTAGAAC ATAGTTCTCA TCCCTATCCA 
CCTGGCCGTC TTTCCCCAAA CAAATCCAAC AATAATAGCA TTGAACATTT GGGTAGTGGT 
AAACGGGACT CGGCATACAG CTCTTTTTCT ACAAGTTCGG GAACCCCAGA TTACACACTG 
TCCAAAAGTA ACACGGCATC CACTGAGAAC ATGCTGTACA AAATAAATCA GTGGGATTCA 
AGTGCCAGGC ACAGCAATGG CAGGCACAGC CAAAACCTAA GCGAAGGGGT ACGACAAGAT 
GAGAGGCCTG GATACCTGCA CCACCTCTCA AGTACAGGTA ACCACGAGAT CCCAAGAGCT 
GAAGAACTGC CTGGCAACCG TCATTCAAGC TCTGGAAGAG TTAATATTGG ACCTGTCTGG 
CATGTTCCAG ATATGAAGAA AAACATGATA TCATCTACTC CACCTCCCAC TCCACCCACA 
CGCAGTGATA GCTTTGCAGC TACCAAGGTT CACGAGAAGG GTCTGATCAC AGGTACTTCA 
GAGGGCCTTG GTGGTCATTC TCAAGTAAAG CCTCAAGCGA AACCACAACA AAAGCCAGGA 
GAAACCCATG AAAGCACACA AAGGTCTAAC CAAGTCATCG AGATTTGTCT TGAGGGTCGA 
CGGGATTATA ATCTACAATC CAAAAATGAT TCCTCAAATA GCTGCATTTC ATCTGATTCT 
CATCATCAGT ATATCCAACG TACATCATCA GATAAGACAT ACTCCCTATC AACCACAGAC 
ATAAGAGATA GCCATCCATC CTATGGCCAT GCTCCATACC ATGCACAGCA GTACAGTGAC 
GAAGGCACTT TCCATGCTCA AACTAGAACA GCAATGAAAC CTCCATTCAG TGGCTATTTC 
AGTAGTATGC AGGAACTGCA CACAAACAAC CACATGCAAC TCAACAGCCA GAATCAAACT 
GGAAGACCAG CTGCATCACT GTCAGTTAAT TTAGGAGTCA ATTCCCACCA TATCTCTCAG 
GGATTAACAC AGGCTTCTTT AATGAGAGCT GATGACTCTA AAGCCTCTTC AGTCTCAGAA 
ATGTCCCACA ATGGACGGGA CAGAATGTCC ATAGGTTCTC AAGGAGGGAA TAAAGAGCGG 
TATTTCCCTC CTCAATCGCA GCACCATGAT AATAAAGAAA GAAGTGCCTC CTTTAAACAA 
AATGAAAACC AACATTATGT TTCTGCACCA AGTAATCCCT TGAATATGTC TGATTCAACA 
AAACATAATG AGCTTAGGGG ATGCCAAAAG CAGCACCATG TGCATGGCTC AGATGAGCTT 
TCTGGTAGTT ATCCACCCAG TAAGCAGCCC GAGCACAGGA GAAGTGCTGG CCATCTTCAT 
CATAAGGAAT ACTCGCAACA ACCCTTTCCA ACTAAAAGCG AATCAAAGAT ATGTCCCCAG 
AAAACACCTA TGTTGTATTC TCTGGCCCAA GGGCACAATG ACACAGATGA CTGTTCTAAT 
CAGATCAAAA GTGGAAGCGC ACAACATGAA GCCCTTGACA GTCTCAGTAG TAAACAGGTT 
AGACGGAATG ACCGATTTGC CACCACTTTG CGCAACGAGA TCCAGATGAG AAGGGCGCAT 
CTCCAAAAGA GTCAAAGTGC AGCCACTTTA GAAAGTCCAG TTGAAACTGT AGAAGAGTCT 
GCTTTGTGGA AGACCACAGG GACTGCTTCT CCCTCATCAG ATGGCTGCTT TTCCATTTCT 
TATAAAGACC ATCTTAAAGA AGCTCAGGCC AGAGTCCTCC AGGCTACATC ATTCAGGAGA 
AAAGACCTAG AGCCAGTATT ATTTGAGCAT CCAGGTACTG AAGGTCCCAC ACGAAAAGAT 
GTACCTCCTT CAAGTGTCTC AGAGGTCACA CCTAGCAAAC CCACCTCAGG GAGTAATCAT 
GTGCTTCGCA TTGGAGGCCG TAAGCGGATA TCTGCTGAAA AGAAAGTGAC GTCTTTTTCT 
GAGCCAGATA AAATTCATGA AGTAGGAGCT GATGAACGCT CCACTATGCC TGAAAATGTT 
GCACACTTAG AAAATCAACA AAGATTCTTT GAGACAACAG GGAAACCAGT TTTCCCTAAG 
CCTATGCCAA AGCAAAACCT GCAGATATCA GAAGACCCCA GACTGTCCAA GCCTGGAGGC 
ATGCACTATT CTGCAAACAG TGATATGGCA GGGCGGAATA ATGGTGAAAG CTCAACCCCC 
ATTGAACACC ACATTAATGA AGGTCTGGAT GGCCCTAACT CAGTGAACCA TCAAGCATTG 
CTAGAACAAC AGCGACTAGG CACATTTGCA GAGTATGAGG CCAAATGGAA CACCCAGAGG 
AAGACTACAG AACCAAGAGT ATCTGGCCGA TACCACTCTG CTGATAACAT CCTCGATACA 
GGAAAAGAGC GACGGAGTAA CCCCACCTGT GTGCATGAAA GATCTAGATC CTCACCTTCT 
GCTGACTTTT ATGGGCAGAA ACTTCCACCA CAAGAAAAGA AGTCAGCTGA CTGTTTCAAA 
TCTGAAAAGA GTCCTTGCCA ACAGGACAAG AATAGTACAA GGTTAAATGA AAAAGGATAT 
AGTGAAATCT TATGCAAGGA GAAACCAGAA ACACCTCCTG AAGAACCTGA GAAAAAGACT 
GTAGATCCTC CCTCCAGCCA TCATAAGCCT GCACTACATT TCCCTGTCCA TAGCACTCGC 
AGTGAACCAT CAGCCCTCTC CAAAAACAAG AGCTCATTTC TCTTGCCCCA TCTGGAGAAG 
TACAAATGCC CTGAGGGAAC ACCTCCTCCT CTTAGCAATA TTCAGGAGGT CCAGCCTGGA 
TCACAAGGAG GAGCTCTGGC CCCAATTTCC CCAGTCAACA ATCAAAGCTC CGCCCCAGTT 
TCAGCCCCCA TTCCTTGGAA GAGTCCAGAG TACAGCAAAG GGCCTGTCGG GGAGGAGGAG 
CAACACCAAG AGCTTGTGCC TCCTCCCTTT CCACCTCCTC CCCCTCCCGT TGTCCTGCCC 
ACCCAACAAA CCGCTGGCCC GACCCAGCCC ACAATGGATG GGCAGCGTTC GCCCTCGCCC 
CAGTTCGCTC CCCAGAGGCT GACTGACAAG CCCCCTGTCT CCGTCTCCTC ACAGGATGAA 
GCTCCTGGAA GGATGGATCA GATTAAAGAT GAGAACACAT CTGTGAAGAA AGTGCCCATT 
AAGATAGTCC ATTCAGAGAG TGACACGGAA AAAGAAAGTC GGCAATATCT AGACCTGCCC 
AGCGAGACGC CTGTCAACTC GCAGGGACTT GTGGGAGCTC ATCTTCAAAG TTTGGGGAAC 
CCGGATCAGT CATACTCTTT GTTTTGTACA TACACCAGAC AAAAAGATCA GGTTCCTGGT 
CTTAGAGACC CAGACATGGG CCCTCTAAAA GACCAGGGGC CACAAAGCAA TATAAACCCA 
GTGTCTTATG TGGAACCTGG CCTTCAGACA GGTCCACCAT TGGATCAGAG TACCAATGGA 
GTGTCTTTGC ATCCTTCACA TTCAGAGGAT GATAAAAGAA AGGAGCTGGC TAGAGACATA 
ATGGACAAAG ACAAGTCCCT CGCTGACATT TTAGACCAGA GTAAGATGAA GACCACTATG 
GATCTGATGG AGGGGATTTT CCCTCAAGGA GATCAGCTAC TGGAGGAGGC CCAACAGCGT 
AGGAAGGCTC AACCTAAACC ACTCTCCCCT CGAAACTCTG TTGAGAAGAA AGAGGAAGAC 
AGCCTGGTGG CGGCCGCTGC TTTAGTGAGC AACTCAACCT ACTACAGCAC ATCTGCACCC 
AAAGCAGAGC TGCTGATCAA GATGAAGGAC ATGCAGGACC AGAGCGTTGA ACACAACTCA 
GAAGAGGAGC TGGAGGAGGA CAATGAAAGT GACCTCGCCT GCAAGAAGCA CGAGTTGATT 
GAAAGTCTCA GTAAGAAGTT GCAGGTCTTA AAAGAAGCTC AGGAGAGCCT GCAGGAGGAC 
GTGCAGGACA ACAACGCTCT TGGAGAAGAA GTGGAGGCCA TCGTACAGGG CGTCTGCAAG 
CCTAATGAAC TCGACAAATT CCGCATGTTC GTCGGGGATC TTGATAAAGT GGTCAACCTT 
CTGCTGTCTC TGTCAGGACG TTTGGCCAGG GTAGAGAATG CCCTGAACAG CCTGGAGGAA 
GATGCTTCGC TGGAGGAAAG GCGCACTCTG ACAGAGAAAC GCAAACTGCT GATTCGTCAG 
CACGAGGATG CTAAGGAGCT GAAGGAGAAC CTGGACAGGA GAGAGCGTGT GGTCTACGAC 
ATTCTGGCCA GTTACCTGCC TGAGGAGAGT ATGGCTGACT ACGAGCACTT TGTCAAGATG 
AAATCCGCTC TTATTATCGA GCAACGCAAG CTGGAGGACA AGATCAAACT GGGAGATGAG 
CAGCTCAAGT GTCTGATGGA CAGTCTCCCA ATGGACCAGA GAATAATCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356635.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212124.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X4, mRNA.

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CloneID	ODa59474
Clone ID Related Accession (Same CDS sequence)	XM_017356635.1
Accession Version	XM_017356635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGAAAGAATC GGTTTAAAGG GAGAAATGAA CCCATGAGCA GACCTCACTC CTGGCATGCC 
ACCAAGTTCA ACGAGACCCA TTCAGAAGCC AAAACCCAGT CCACACCCTC ACCAGTTTGG 
CAAACAAGAT ATGATGCAAG TTCATTCTCT TCTGAACTCT CAACTGGCTG GGATCAAACA 
AATCTACGGA GAGTATCCGA TCAGTTCAGT TCTTTGGGGA GTATGGACAG CCTAGAACAT 
AGTTCTCATC CCTATCCACC TGGCCGTCTT TCCCCAAACA AATCCAACAA TAATAGCATT 
GAACATTTGG GTAGTGGTAA ACGGGACTCG GCATACAGCT CTTTTTCTAC AAGTTCGGGA 
ACCCCAGATT ACACACTGTC CAAAAGTAAC ACGGCATCCA CTGAGAACAT GCTGTACAAA 
ATAAATCAGT GGGATTCAAG TGCCAGGCAC AGCAATGGCA GGCACAGCCA AAACCTAAGC 
GAAGGGGTAC GACAAGATGA GAGGCCTGGA TACCTGCACC ACCTCTCAAG TACAGGTAAC 
CACGAGATCC CAAGAGCTGA AGAACTGCCT GGCAACCGTC ATTCAAGCTC TGGAAGAGTT 
AATATTGGAC CTGTCTGGCA TGTTCCAGAT ATGAAGAAAA ACATGATATC ATCTACTCCA 
CCTCCCACTC CACCCACACG CAGTGATAGC TTTGCAGCTA CCAAGGTTCA CGAGAAGGGT 
CTGATCACAG GTACTTCAGA GGGCCTTGGT GGTCATTCTC AAGTAAAGCC TCAAGCGAAA 
CCACAACAAA AGCCAGGAGA AACCCATGAA AGCACACAAA GGTCTAACCA AGTCATCGAG 
ATTTGTCTTG AGGGTCGACG GGATTATAAT CTACAATCCA AAAATGATTC CTCAAATAGC 
TGCATTTCAT CTGATTCTCA TCATCAGTAT ATCCAACGTA CATCATCAGA TAAGACATAC 
TCCCTATCAA CCACAGACAT AAGAGATAGC CATCCATCCT ATGGCCATGC TCCATACCAT 
GCACAGCAGT ACAGTGACGA AGGCACTTTC CATGCTCAAA CTAGAACAGC AATGAAACCT 
CCATTCAGTG GCTATTTCAG TAGTATGCAG GAACTGCACA CAAACAACCA CATGCAACTC 
AACAGCCAGA ATCAAACTGG AAGACCAGCT GCATCACTGT CAGTTAATTT AGGAGTCAAT 
TCCCACCATA TCTCTCAGGG ATTAACACAG GCTTCTTTAA TGAGAGCTGA TGACTCTAAA 
GCCTCTTCAG TCTCAGAAAT GTCCCACAAT GGACGGGACA GAATGTCCAT AGGTTCTCAA 
GGAGGGAATA AAGAGCGGTA TTTCCCTCCT CAATCGCAGC ACCATGATAA TAAAGAAAGA 
AGTGCCTCCT TTAAACAAAA TGAAAACCAA CATTATGTTT CTGCACCAAG TAATCCCTTG 
AATATGTCTG ATTCAACAAA ACATAATGAG CTTAGGGGAT GCCAAAAGCA GCACCATGTG 
CATGGCTCAG ATGAGCTTTC TGGTAGTTAT CCACCCAGTA AGCAGCCCGA GCACAGGAGA 
AGTGCTGGCC ATCTTCATCA TAAGGAATAC TCGCAACAAC CCTTTCCAAC TAAAAGCGAA 
TCAAAGATAT GTCCCCAGAA AACACCTATG TTGTATTCTC TGGCCCAAGG GCACAATGAC 
ACAGATGACT GTTCTAATCA GATCAAAAGT GGAAGCGCAC AACATGAAGC CCTTGACAGT 
CTCAGTAGTA AACAGGTTAG ACGGAATGAC CGATTTGCCA CCACTTTGCG CAACGAGATC 
CAGATGAGAA GGGCGCATCT CCAAAAGAGT CAAAGTGCAG CCACTTTAGA AAGTCCAGTT 
GAAACTGTAG AAGAGTCTGC TTTGTGGAAG ACCACAGGGA CTGCTTCTCC CTCATCAGAT 
GGCTGCTTTT CCATTTCTTA TAAAGACCAT CTTAAAGAAG CTCAGGCCAG AGTCCTCCAG 
GCTACATCAT TCAGGAGAAA AGACCTAGAG CCAGTATTAT TTGAGCATCC AGGTACTGAA 
GGTCCCACAC GAAAAGATGT ACCTCCTTCA AGTGTCTCAG AGGTCACACC TAGCAAACCC 
ACCTCAGGGA GTAATCATGT GCTTCGCATT GGAGGCCGTA AGCGGATATC TGCTGAAAAG 
AAAGTGACGT CTTTTTCTGA GCCAGATAAA ATTCATGAAG TAGGAGCTGA TGAACGCTCC 
ACTATGCCTG AAAATGTTGC ACACTTAGAA AATCAACAAA GATTCTTTGA GACAACAGGG 
AAACCAGTTT TCCCTAAGCC TATGCCAAAG CAAAACCTGC AGATATCAGA AGACCCCAGA 
CTGTCCAAGC CTGGAGGCAT GCACTATTCT GCAAACAGTG ATATGGCAGG GCGGAATAAT 
GGTGAAAGCT CAACCCCCAT TGAACACCAC ATTAATGAAG GTCTGGATGG CCCTAACTCA 
GTGAACCATC AAGCATTGCT AGAACAACAG CGACTAGGCA CATTTGCAGA GTATGAGGCC 
AAATGGAACA CCCAGAGGAA GACTACAGAA CCAAGAGTAT CTGGCCGATA CCACTCTGCT 
GATAACATCC TCGATACAGG AAAAGAGCGA CGGAGTAACC CCACCTGTGT GCATGAAAGA 
TCTAGATCCT CACCTTCTGC TGACTTTTAT GGGCAGAAAC TTCCACCACA AGAAAAGAAG 
TCAGCTGACT GTTTCAAATC TGAAAAGAGT CCTTGCCAAC AGGACAAGAA TAGTACAAGT 
CAAAATTTCT ACCCATCTGT TTCTTTGGAG CACAGCCACT GGTTCTATAC ACCCAGCAGG 
TTAAATGAAA AAGGATATAG TGAAATCTTA TGCAAGGAGA AACCAGAAAC ACCTCCTGAA 
GAACCTGAGA AAAAGACTGT AGATCCTCCC TCCAGCCATC ATAAGCCTGC ACTACATTTC 
CCTGTCCATA GCACTCGCAG TGAACCATCA GCCCTCTCCA AAAACAAGAG CTCATTTCTC 
TTGCCCCATC TGGAGAAGTA CAAATGCCCT GAGGGAACAC CTCCTCCTCT TAGCAATATT 
CAGGAGGTCC AGCCTGGATC ACAAGGAGGA GCTCTGGCCC CAATTTCCCC AGTCAACAAT 
CAAAGCTCCG CCCCAGTTTC AGCCCCCATT CCTTGGAAGA GTCCAGAGTA CAGCAAAGGG 
CCTGTCGGGG AGGAGGAGCA ACACCAAGAG CTTGTGCCTC CTCCCTTTCC ACCTCCTCCC 
CCTCCCGTTG TCCTGCCCAC CCAACAAACC GCTGGCCCGA CCCAGCCCAC AATGGATGGG 
CAGCGTTCGC CCTCGCCCCA GTTCGCTCCC CAGAGGCTGA CTGACAAGCC CCCTGTCTCC 
GTCTCCTCAC AGGATGAAGC TCCTGGAAGG ATGGATCAGA TTAAAGATGA GAACACATCT 
GTGAAGAAAG TGCCCATTAA GATAGTCCAT TCAGAGAGTG ACACGGAAAA AGAAAGTCGG 
CAATATCTAG ACCTGCCCAG CGAGACGCCT GTCAACTCGC AGGGACTTGT GGGAGCTCAT 
CTTCAAAGTT TGGGGAACCC GGATCAGTCA TACTCTTTGT TTTGTACATA CACCAGACAA 
AAAGATCAGG TTCCTGGTCT TAGAGACCCA GACATGGGCC CTCTAAAAGA CCAGGGGCCA 
CAAAGCAATA TAAACCCAGT GTCTTATGTG GAACCTGGCC TTCAGACAGG TCCACCATTG 
GATCAGAGTA CCAATGGAGT GTCTTTGCAT CCTTCACATT CAGAGGATGA TAAAAGAAAG 
GAGCTGGCTA GAGACATAAT GGACAAAGAC AAGTCCCTCG CTGACATTTT AGACCAGAGT 
AAGATGAAGA CCACTATGGA TCTGATGGAG GGGATTTTCC CTCAAGGAGA TCAGCTACTG 
GAGGAGGCCC AACAGCGTAG GAAGGCTCAA CCTAAACCAC TCTCCCCTCG AAACTCTGTT 
GAGAAGAAAG AGGAAGACAG CCTGGTGGCG GCCGCTGCTT TAGTGAGCAA CTCAACCTAC 
TACAGCACAT CTGCACCCAA AGCAGAGCTG CTGATCAAGA TGAAGGACAT GCAGGACCAG 
AGCGTTGAAC ACAACTCAGA AGAGGAGCTG GAGGAGGACA ATGAAAGTGA CCTCGCCTGC 
AAGAAGCACG AGTTGATTGA AAGTCTCAGT AAGAAGTTGC AGGTCTTAAA AGAAGCTCAG 
GAGAGCCTGC AGGAGGACGT GCAGGACAAC AACGCTCTTG GAGAAGAAGT GGAGGCCATC 
GTACAGGGCG TCTGCAAGCC TAATGAACTC GACAAATTCC GCATGTTCGT CGGGGATCTT 
GATAAAGTGG TCAACCTTCT GCTGTCTCTG TCAGGACGTT TGGCCAGGGT AGAGAATGCC 
CTGAACAGCC TGGAGGAAGA TGCTTCGCTG GAGGAAAGGC GCACTCTGAC AGAGAAACGC 
AAACTGCTGA TTCGTCAGCA CGAGGATGCT AAGGAGCTGA AGGAGAACCT GGACAGGAGA 
GAGCGTGTGG TCTACGACAT TCTGGCCAGT TACCTGCCTG AGGAGAGTAT GGCTGACTAC 
GAGCACTTTG TCAAGATGAA ATCCGCTCTT ATTATCGAGC AACGCAAGCT GGAGGACAAG 
ATCAAACTGG GAGATGAGCA GCTCAAGTGT CTGATGGACA GTCTCCCAAT GGACCAGAGA 
ATAATCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212124.1
CDS	766..5817
Translation	MDTINQYPVNPSFPEDDHRSFCSSIRSASFNEHHVNDEGWKVVEVTLSGGAPWGFTLRGGLEHGEPLLITKVEEGSKAATVRLQVGDELININNISLTGFRQEAICLVKSSYKTLSLVVKRKNRFKGRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356635.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356635.1

ORF Insert Sequence:

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGAAAGAATC GGTTTAAAGG GAGAAATGAA CCCATGAGCA GACCTCACTC CTGGCATGCC 
ACCAAGTTCA ACGAGACCCA TTCAGAAGCC AAAACCCAGT CCACACCCTC ACCAGTTTGG 
CAAACAAGAT ATGATGCAAG TTCATTCTCT TCTGAACTCT CAACTGGCTG GGATCAAACA 
AATCTACGGA GAGTATCCGA TCAGTTCAGT TCTTTGGGGA GTATGGACAG CCTAGAACAT 
AGTTCTCATC CCTATCCACC TGGCCGTCTT TCCCCAAACA AATCCAACAA TAATAGCATT 
GAACATTTGG GTAGTGGTAA ACGGGACTCG GCATACAGCT CTTTTTCTAC AAGTTCGGGA 
ACCCCAGATT ACACACTGTC CAAAAGTAAC ACGGCATCCA CTGAGAACAT GCTGTACAAA 
ATAAATCAGT GGGATTCAAG TGCCAGGCAC AGCAATGGCA GGCACAGCCA AAACCTAAGC 
GAAGGGGTAC GACAAGATGA GAGGCCTGGA TACCTGCACC ACCTCTCAAG TACAGGTAAC 
CACGAGATCC CAAGAGCTGA AGAACTGCCT GGCAACCGTC ATTCAAGCTC TGGAAGAGTT 
AATATTGGAC CTGTCTGGCA TGTTCCAGAT ATGAAGAAAA ACATGATATC ATCTACTCCA 
CCTCCCACTC CACCCACACG CAGTGATAGC TTTGCAGCTA CCAAGGTTCA CGAGAAGGGT 
CTGATCACAG GTACTTCAGA GGGCCTTGGT GGTCATTCTC AAGTAAAGCC TCAAGCGAAA 
CCACAACAAA AGCCAGGAGA AACCCATGAA AGCACACAAA GGTCTAACCA AGTCATCGAG 
ATTTGTCTTG AGGGTCGACG GGATTATAAT CTACAATCCA AAAATGATTC CTCAAATAGC 
TGCATTTCAT CTGATTCTCA TCATCAGTAT ATCCAACGTA CATCATCAGA TAAGACATAC 
TCCCTATCAA CCACAGACAT AAGAGATAGC CATCCATCCT ATGGCCATGC TCCATACCAT 
GCACAGCAGT ACAGTGACGA AGGCACTTTC CATGCTCAAA CTAGAACAGC AATGAAACCT 
CCATTCAGTG GCTATTTCAG TAGTATGCAG GAACTGCACA CAAACAACCA CATGCAACTC 
AACAGCCAGA ATCAAACTGG AAGACCAGCT GCATCACTGT CAGTTAATTT AGGAGTCAAT 
TCCCACCATA TCTCTCAGGG ATTAACACAG GCTTCTTTAA TGAGAGCTGA TGACTCTAAA 
GCCTCTTCAG TCTCAGAAAT GTCCCACAAT GGACGGGACA GAATGTCCAT AGGTTCTCAA 
GGAGGGAATA AAGAGCGGTA TTTCCCTCCT CAATCGCAGC ACCATGATAA TAAAGAAAGA 
AGTGCCTCCT TTAAACAAAA TGAAAACCAA CATTATGTTT CTGCACCAAG TAATCCCTTG 
AATATGTCTG ATTCAACAAA ACATAATGAG CTTAGGGGAT GCCAAAAGCA GCACCATGTG 
CATGGCTCAG ATGAGCTTTC TGGTAGTTAT CCACCCAGTA AGCAGCCCGA GCACAGGAGA 
AGTGCTGGCC ATCTTCATCA TAAGGAATAC TCGCAACAAC CCTTTCCAAC TAAAAGCGAA 
TCAAAGATAT GTCCCCAGAA AACACCTATG TTGTATTCTC TGGCCCAAGG GCACAATGAC 
ACAGATGACT GTTCTAATCA GATCAAAAGT GGAAGCGCAC AACATGAAGC CCTTGACAGT 
CTCAGTAGTA AACAGGTTAG ACGGAATGAC CGATTTGCCA CCACTTTGCG CAACGAGATC 
CAGATGAGAA GGGCGCATCT CCAAAAGAGT CAAAGTGCAG CCACTTTAGA AAGTCCAGTT 
GAAACTGTAG AAGAGTCTGC TTTGTGGAAG ACCACAGGGA CTGCTTCTCC CTCATCAGAT 
GGCTGCTTTT CCATTTCTTA TAAAGACCAT CTTAAAGAAG CTCAGGCCAG AGTCCTCCAG 
GCTACATCAT TCAGGAGAAA AGACCTAGAG CCAGTATTAT TTGAGCATCC AGGTACTGAA 
GGTCCCACAC GAAAAGATGT ACCTCCTTCA AGTGTCTCAG AGGTCACACC TAGCAAACCC 
ACCTCAGGGA GTAATCATGT GCTTCGCATT GGAGGCCGTA AGCGGATATC TGCTGAAAAG 
AAAGTGACGT CTTTTTCTGA GCCAGATAAA ATTCATGAAG TAGGAGCTGA TGAACGCTCC 
ACTATGCCTG AAAATGTTGC ACACTTAGAA AATCAACAAA GATTCTTTGA GACAACAGGG 
AAACCAGTTT TCCCTAAGCC TATGCCAAAG CAAAACCTGC AGATATCAGA AGACCCCAGA 
CTGTCCAAGC CTGGAGGCAT GCACTATTCT GCAAACAGTG ATATGGCAGG GCGGAATAAT 
GGTGAAAGCT CAACCCCCAT TGAACACCAC ATTAATGAAG GTCTGGATGG CCCTAACTCA 
GTGAACCATC AAGCATTGCT AGAACAACAG CGACTAGGCA CATTTGCAGA GTATGAGGCC 
AAATGGAACA CCCAGAGGAA GACTACAGAA CCAAGAGTAT CTGGCCGATA CCACTCTGCT 
GATAACATCC TCGATACAGG AAAAGAGCGA CGGAGTAACC CCACCTGTGT GCATGAAAGA 
TCTAGATCCT CACCTTCTGC TGACTTTTAT GGGCAGAAAC TTCCACCACA AGAAAAGAAG 
TCAGCTGACT GTTTCAAATC TGAAAAGAGT CCTTGCCAAC AGGACAAGAA TAGTACAAGT 
CAAAATTTCT ACCCATCTGT TTCTTTGGAG CACAGCCACT GGTTCTATAC ACCCAGCAGG 
TTAAATGAAA AAGGATATAG TGAAATCTTA TGCAAGGAGA AACCAGAAAC ACCTCCTGAA 
GAACCTGAGA AAAAGACTGT AGATCCTCCC TCCAGCCATC ATAAGCCTGC ACTACATTTC 
CCTGTCCATA GCACTCGCAG TGAACCATCA GCCCTCTCCA AAAACAAGAG CTCATTTCTC 
TTGCCCCATC TGGAGAAGTA CAAATGCCCT GAGGGAACAC CTCCTCCTCT TAGCAATATT 
CAGGAGGTCC AGCCTGGATC ACAAGGAGGA GCTCTGGCCC CAATTTCCCC AGTCAACAAT 
CAAAGCTCCG CCCCAGTTTC AGCCCCCATT CCTTGGAAGA GTCCAGAGTA CAGCAAAGGG 
CCTGTCGGGG AGGAGGAGCA ACACCAAGAG CTTGTGCCTC CTCCCTTTCC ACCTCCTCCC 
CCTCCCGTTG TCCTGCCCAC CCAACAAACC GCTGGCCCGA CCCAGCCCAC AATGGATGGG 
CAGCGTTCGC CCTCGCCCCA GTTCGCTCCC CAGAGGCTGA CTGACAAGCC CCCTGTCTCC 
GTCTCCTCAC AGGATGAAGC TCCTGGAAGG ATGGATCAGA TTAAAGATGA GAACACATCT 
GTGAAGAAAG TGCCCATTAA GATAGTCCAT TCAGAGAGTG ACACGGAAAA AGAAAGTCGG 
CAATATCTAG ACCTGCCCAG CGAGACGCCT GTCAACTCGC AGGGACTTGT GGGAGCTCAT 
CTTCAAAGTT TGGGGAACCC GGATCAGTCA TACTCTTTGT TTTGTACATA CACCAGACAA 
AAAGATCAGG TTCCTGGTCT TAGAGACCCA GACATGGGCC CTCTAAAAGA CCAGGGGCCA 
CAAAGCAATA TAAACCCAGT GTCTTATGTG GAACCTGGCC TTCAGACAGG TCCACCATTG 
GATCAGAGTA CCAATGGAGT GTCTTTGCAT CCTTCACATT CAGAGGATGA TAAAAGAAAG 
GAGCTGGCTA GAGACATAAT GGACAAAGAC AAGTCCCTCG CTGACATTTT AGACCAGAGT 
AAGATGAAGA CCACTATGGA TCTGATGGAG GGGATTTTCC CTCAAGGAGA TCAGCTACTG 
GAGGAGGCCC AACAGCGTAG GAAGGCTCAA CCTAAACCAC TCTCCCCTCG AAACTCTGTT 
GAGAAGAAAG AGGAAGACAG CCTGGTGGCG GCCGCTGCTT TAGTGAGCAA CTCAACCTAC 
TACAGCACAT CTGCACCCAA AGCAGAGCTG CTGATCAAGA TGAAGGACAT GCAGGACCAG 
AGCGTTGAAC ACAACTCAGA AGAGGAGCTG GAGGAGGACA ATGAAAGTGA CCTCGCCTGC 
AAGAAGCACG AGTTGATTGA AAGTCTCAGT AAGAAGTTGC AGGTCTTAAA AGAAGCTCAG 
GAGAGCCTGC AGGAGGACGT GCAGGACAAC AACGCTCTTG GAGAAGAAGT GGAGGCCATC 
GTACAGGGCG TCTGCAAGCC TAATGAACTC GACAAATTCC GCATGTTCGT CGGGGATCTT 
GATAAAGTGG TCAACCTTCT GCTGTCTCTG TCAGGACGTT TGGCCAGGGT AGAGAATGCC 
CTGAACAGCC TGGAGGAAGA TGCTTCGCTG GAGGAAAGGC GCACTCTGAC AGAGAAACGC 
AAACTGCTGA TTCGTCAGCA CGAGGATGCT AAGGAGCTGA AGGAGAACCT GGACAGGAGA 
GAGCGTGTGG TCTACGACAT TCTGGCCAGT TACCTGCCTG AGGAGAGTAT GGCTGACTAC 
GAGCACTTTG TCAAGATGAA ATCCGCTCTT ATTATCGAGC AACGCAAGCT GGAGGACAAG 
ATCAAACTGG GAGATGAGCA GCTCAAGTGT CTGATGGACA GTCTCCCAAT GGACCAGAGA 
ATAATCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356636.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212125.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X5, mRNA.

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CloneID	ODa59475
Clone ID Related Accession (Same CDS sequence)	XM_017356636.1 , XM_017356636.2
Accession Version	XM_017356636.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom2 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAGAAATG AACCCATGAG CAGACCTCAC TCCTGGCATG CCACCAAGTT CAACGAGACC 
CATTCAGAAG CCAAAACCCA GTCCACACCC TCACCAGTTT GGCAAACAAG ATATGATGCA 
AGTTCATTCT CTTCTGAACT CTCAACTGGC TGGGATCAAA CAAATCTACG GAGAGTATCC 
GATCAGTTCA GTTCTTTGGG GAGTATGGAC AGCCTAGAAC ATAGTTCTCA TCCCTATCCA 
CCTGGCCGTC TTTCCCCAAA CAAATCCAAC AATAATAGCA TTGAACATTT GGGTAGTGGT 
AAACGGGACT CGGCATACAG CTCTTTTTCT ACAAGTTCGG GAACCCCAGA TTACACACTG 
TCCAAAAGTA ACACGGCATC CACTGAGAAC ATGCTGTACA AAATAAATCA GTGGGATTCA 
AGTGCCAGGC ACAGCAATGG CAGGCACAGC CAAAACCTAA GCGAAGGGGT ACGACAAGAT 
GAGAGGCCTG GATACCTGCA CCACCTCTCA AGTACAGGTA ACCACGAGAT CCCAAGAGCT 
GAAGAACTGC CTGGCAACCG TCATTCAAGC TCTGGAAGAG TTAATATTGG ACCTGTCTGG 
CATGTTCCAG ATATGAAGAA AAACATGATA TCATCTACTC CACCTCCCAC TCCACCCACA 
CGCAGTGATA GCTTTGCAGC TACCAAGGTT CACGAGAAGG GTCTGATCAC AGGTACTTCA 
GAGGGCCTTG GTGGTCATTC TCAAGTAAAG CCTCAAGCGA AACCACAACA AAAGCCAGGA 
GAAACCCATG AAAGCACACA AAGGTCTAAC CAAGTCATCG AGATTTGTCT TGAGGGTCGA 
CGGGATTATA ATCTACAATC CAAAAATGAT TCCTCAAATA GCTGCATTTC ATCTGATTCT 
CATCATCAGT ATATCCAACG TACATCATCA GATAAGACAT ACTCCCTATC AACCACAGAC 
ATAAGAGATA GCCATCCATC CTATGGCCAT GCTCCATACC ATGCACAGCA GTACAGTGAC 
GAAGGCACTT TCCATGCTCA AACTAGAACA GCAATGAAAC CTCCATTCAG TGGCTATTTC 
AGTAGTATGC AGGAACTGCA CACAAACAAC CACATGCAAC TCAACAGCCA GAATCAAACT 
GGAAGACCAG CTGCATCACT GTCAGTTAAT TTAGGAGTCA ATTCCCACCA TATCTCTCAG 
GGATTAACAC AGGCTTCTTT AATGAGAGCT GATGACTCTA AAGCCTCTTC AGTCTCAGAA 
ATGTCCCACA ATGGACGGGA CAGAATGTCC ATAGGTTCTC AAGGAGGGAA TAAAGAGCGG 
TATTTCCCTC CTCAATCGCA GCACCATGAT AATAAAGAAA GAAGTGCCTC CTTTAAACAA 
AATGAAAACC AACATTATGT TTCTGCACCA AGTAATCCCT TGAATATGTC TGATTCAACA 
AAACATAATG AGCTTAGGGG ATGCCAAAAG CAGCACCATG TGCATGGCTC AGATGAGCTT 
TCTGGTAGTT ATCCACCCAG TAAGCAGCCC GAGCACAGGA GAAGTGCTGG CCATCTTCAT 
CATAAGGAAT ACTCGCAACA ACCCTTTCCA ACTAAAAGCG AATCAAAGAT ATGTCCCCAG 
AAAACACCTA TGTTGTATTC TCTGGCCCAA GGGCACAATG ACACAGATGA CTGTTCTAAT 
CAGATCAAAA GTGGAAGCGC ACAACATGAA GCCCTTGACA GTCTCAGTAG TAAACAGGTT 
AGACGGAATG ACCGATTTGC CACCACTTTG CGCAACGAGA TCCAGATGAG AAGGGCGCAT 
CTCCAAAAGA GTCAAAGTGC AGCCACTTTA GAAAGTCCAG TTGAAACTGT AGAAGAGTCT 
GCTTTGTGGA AGACCACAGG GACTGCTTCT CCCTCATCAG ATGGCTGCTT TTCCATTTCT 
TATAAAGACC ATCTTAAAGA AGCTCAGGCC AGAGTCCTCC AGGCTACATC ATTCAGGAGA 
AAAGACCTAG AGCCAGTATT ATTTGAGCAT CCAGGTACTG AAGGTCCCAC ACGAAAAGAT 
GTACCTCCTT CAAGTGTCTC AGAGGTCACA CCTAGCAAAC CCACCTCAGG GAGTAATCAT 
GTGCTTCGCA TTGGAGGCCG TAAGCGGATA TCTGCTGAAA AGAAAGTGAC GTCTTTTTCT 
GAGCCAGATA AAATTCATGA AGTAGGAGCT GATGAACGCT CCACTATGCC TGAAAATGTT 
GCACACTTAG AAAATCAACA AAGATTCTTT GAGACAACAG GGAAACCAGT TTTCCCTAAG 
CCTATGCCAA AGCAAAACCT GCAGATATCA GAAGACCCCA GACTGTCCAA GCCTGGAGGC 
ATGCACTATT CTGCAAACAG TGATATGGCA GGGCGGAATA ATGGTGAAAG CTCAACCCCC 
ATTGAACACC ACATTAATGA AGGTCTGGAT GGCCCTAACT CAGTGAACCA TCAAGCATTG 
CTAGAACAAC AGCGACTAGG CACATTTGCA GAGTATGAGG CCAAATGGAA CACCCAGAGG 
AAGACTACAG AACCAAGAGT ATCTGGCCGA TACCACTCTG CTGATAACAT CCTCGATACA 
GGAAAAGAGC GACGGAGTAA CCCCACCTGT GTGCATGAAA GATCTAGATC CTCACCTTCT 
GCTGACTTTT ATGGGCAGAA ACTTCCACCA CAAGAAAAGA AGTCAGCTGA CTGTTTCAAA 
TCTGAAAAGA GTCCTTGCCA ACAGGACAAG AATAGTACAA GTCAAAATTT CTACCCATCT 
GTTTCTTTGG AGCACAGCCA CTGGTTCTAT ACACCCAGCA GGTTAAATGA AAAAGGATAT 
AGTGAAATCT TATGCAAGGA GAAACCAGAA ACACCTCCTG AAGAACCTGA GAAAAAGACT 
GTAGATCCTC CCTCCAGCCA TCATAAGCCT GCACTACATT TCCCTGTCCA TAGCACTCGC 
AGTGAACCAT CAGCCCTCTC CAAAAACAAG AGCTCATTTC TCTTGCCCCA TCTGGAGAAG 
TACAAATGCC CTGAGGGAAC ACCTCCTCCT CTTAGCAATA TTCAGGAGGT CCAGCCTGGA 
TCACAAGGAG GAGCTCTGGC CCCAATTTCC CCAGTCAACA ATCAAAGCTC CGCCCCAGTT 
TCAGCCCCCA TTCCTTGGAA GAGTCCAGAG TACAGCAAAG GGCCTGTCGG GGAGGAGGAG 
CAACACCAAG AGCTTGTGCC TCCTCCCTTT CCACCTCCTC CCCCTCCCGT TGTCCTGCCC 
ACCCAACAAA CCGCTGGCCC GACCCAGCCC ACAATGGATG GGCAGCGTTC GCCCTCGCCC 
CAGTTCGCTC CCCAGAGGCT GACTGACAAG CCCCCTGTCT CCGTCTCCTC ACAGGATGAA 
GCTCCTGGAA GGATGGATCA GATTAAAGAT GAGAACACAT CTGTGAAGAA AGTGCCCATT 
AAGATAGTCC ATTCAGAGAG TGACACGGAA AAAGAAAGTC GGCAATATCT AGACCTGCCC 
AGCGAGACGC CTGTCAACTC GCAGGGACTT GTGGGAGCTC ATCTTCAAAG TTTGGGGAAC 
CCGGATCAGT CATACTCTTT GTTTTGTACA TACACCAGAC AAAAAGATCA GGTTCCTGGT 
CTTAGAGACC CAGACATGGG CCCTCTAAAA GACCAGGGGC CACAAAGCAA TATAAACCCA 
GTGTCTTATG TGGAACCTGG CCTTCAGACA GGTCCACCAT TGGATCAGAG TACCAATGGA 
GTGTCTTTGC ATCCTTCACA TTCAGAGGAT GATAAAAGAA AGGAGCTGGC TAGAGACATA 
ATGGACAAAG ACAAGTCCCT CGCTGACATT TTAGACCAGA GTAAGATGAA GACCACTATG 
GATCTGATGG AGGGGATTTT CCCTCAAGGA GATCAGCTAC TGGAGGAGGC CCAACAGCGT 
AGGAAGGCTC AACCTAAACC ACTCTCCCCT CGAAACTCTG TTGAGAAGAA AGAGGAAGAC 
AGCCTGGTGG CGGCCGCTGC TTTAGTGAGC AACTCAACCT ACTACAGCAC ATCTGCACCC 
AAAGCAGAGC TGCTGATCAA GATGAAGGAC ATGCAGGACC AGAGCGTTGA ACACAACTCA 
GAAGAGGAGC TGGAGGAGGA CAATGAAAGT GACCTCGCCT GCAAGAAGCA CGAGTTGATT 
GAAAGTCTCA GTAAGAAGTT GCAGGTCTTA AAAGAAGCTC AGGAGAGCCT GCAGGAGGAC 
GTGCAGGACA ACAACGCTCT TGGAGAAGAA GTGGAGGCCA TCGTACAGGG CGTCTGCAAG 
CCTAATGAAC TCGACAAATT CCGCATGTTC GTCGGGGATC TTGATAAAGT GGTCAACCTT 
CTGCTGTCTC TGTCAGGACG TTTGGCCAGG GTAGAGAATG CCCTGAACAG CCTGGAGGAA 
GATGCTTCGC TGGAGGAAAG GCGCACTCTG ACAGAGAAAC GCAAACTGCT GATTCGTCAG 
CACGAGGATG CTAAGGAGCT GAAGGAGAAC CTGGACAGGA GAGAGCGTGT GGTCTACGAC 
ATTCTGGCCA GTTACCTGCC TGAGGAGAGT ATGGCTGACT ACGAGCACTT TGTCAAGATG 
AAATCCGCTC TTATTATCGA GCAACGCAAG CTGGAGGACA AGATCAAACT GGGAGATGAG 
CAGCTCAAGT GTCTGATGGA CAGTCTCCCA ATGGACCAGA GAATAATCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212125.1
CDS	766..5799
Translation	MDTINQYPVNPSFPEDDHRSFCSSIRSASFNEHHVNDEGWKVVEVTLSGGAPWGFTLRGGLEHGEPLLITKVEEGSKAATVRLQVGDELININNISLTGFRQEAICLVKSSYKTLSLVVKRRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356636.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356636.1

ORF Insert Sequence:

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAGAAATG AACCCATGAG CAGACCTCAC TCCTGGCATG CCACCAAGTT CAACGAGACC 
CATTCAGAAG CCAAAACCCA GTCCACACCC TCACCAGTTT GGCAAACAAG ATATGATGCA 
AGTTCATTCT CTTCTGAACT CTCAACTGGC TGGGATCAAA CAAATCTACG GAGAGTATCC 
GATCAGTTCA GTTCTTTGGG GAGTATGGAC AGCCTAGAAC ATAGTTCTCA TCCCTATCCA 
CCTGGCCGTC TTTCCCCAAA CAAATCCAAC AATAATAGCA TTGAACATTT GGGTAGTGGT 
AAACGGGACT CGGCATACAG CTCTTTTTCT ACAAGTTCGG GAACCCCAGA TTACACACTG 
TCCAAAAGTA ACACGGCATC CACTGAGAAC ATGCTGTACA AAATAAATCA GTGGGATTCA 
AGTGCCAGGC ACAGCAATGG CAGGCACAGC CAAAACCTAA GCGAAGGGGT ACGACAAGAT 
GAGAGGCCTG GATACCTGCA CCACCTCTCA AGTACAGGTA ACCACGAGAT CCCAAGAGCT 
GAAGAACTGC CTGGCAACCG TCATTCAAGC TCTGGAAGAG TTAATATTGG ACCTGTCTGG 
CATGTTCCAG ATATGAAGAA AAACATGATA TCATCTACTC CACCTCCCAC TCCACCCACA 
CGCAGTGATA GCTTTGCAGC TACCAAGGTT CACGAGAAGG GTCTGATCAC AGGTACTTCA 
GAGGGCCTTG GTGGTCATTC TCAAGTAAAG CCTCAAGCGA AACCACAACA AAAGCCAGGA 
GAAACCCATG AAAGCACACA AAGGTCTAAC CAAGTCATCG AGATTTGTCT TGAGGGTCGA 
CGGGATTATA ATCTACAATC CAAAAATGAT TCCTCAAATA GCTGCATTTC ATCTGATTCT 
CATCATCAGT ATATCCAACG TACATCATCA GATAAGACAT ACTCCCTATC AACCACAGAC 
ATAAGAGATA GCCATCCATC CTATGGCCAT GCTCCATACC ATGCACAGCA GTACAGTGAC 
GAAGGCACTT TCCATGCTCA AACTAGAACA GCAATGAAAC CTCCATTCAG TGGCTATTTC 
AGTAGTATGC AGGAACTGCA CACAAACAAC CACATGCAAC TCAACAGCCA GAATCAAACT 
GGAAGACCAG CTGCATCACT GTCAGTTAAT TTAGGAGTCA ATTCCCACCA TATCTCTCAG 
GGATTAACAC AGGCTTCTTT AATGAGAGCT GATGACTCTA AAGCCTCTTC AGTCTCAGAA 
ATGTCCCACA ATGGACGGGA CAGAATGTCC ATAGGTTCTC AAGGAGGGAA TAAAGAGCGG 
TATTTCCCTC CTCAATCGCA GCACCATGAT AATAAAGAAA GAAGTGCCTC CTTTAAACAA 
AATGAAAACC AACATTATGT TTCTGCACCA AGTAATCCCT TGAATATGTC TGATTCAACA 
AAACATAATG AGCTTAGGGG ATGCCAAAAG CAGCACCATG TGCATGGCTC AGATGAGCTT 
TCTGGTAGTT ATCCACCCAG TAAGCAGCCC GAGCACAGGA GAAGTGCTGG CCATCTTCAT 
CATAAGGAAT ACTCGCAACA ACCCTTTCCA ACTAAAAGCG AATCAAAGAT ATGTCCCCAG 
AAAACACCTA TGTTGTATTC TCTGGCCCAA GGGCACAATG ACACAGATGA CTGTTCTAAT 
CAGATCAAAA GTGGAAGCGC ACAACATGAA GCCCTTGACA GTCTCAGTAG TAAACAGGTT 
AGACGGAATG ACCGATTTGC CACCACTTTG CGCAACGAGA TCCAGATGAG AAGGGCGCAT 
CTCCAAAAGA GTCAAAGTGC AGCCACTTTA GAAAGTCCAG TTGAAACTGT AGAAGAGTCT 
GCTTTGTGGA AGACCACAGG GACTGCTTCT CCCTCATCAG ATGGCTGCTT TTCCATTTCT 
TATAAAGACC ATCTTAAAGA AGCTCAGGCC AGAGTCCTCC AGGCTACATC ATTCAGGAGA 
AAAGACCTAG AGCCAGTATT ATTTGAGCAT CCAGGTACTG AAGGTCCCAC ACGAAAAGAT 
GTACCTCCTT CAAGTGTCTC AGAGGTCACA CCTAGCAAAC CCACCTCAGG GAGTAATCAT 
GTGCTTCGCA TTGGAGGCCG TAAGCGGATA TCTGCTGAAA AGAAAGTGAC GTCTTTTTCT 
GAGCCAGATA AAATTCATGA AGTAGGAGCT GATGAACGCT CCACTATGCC TGAAAATGTT 
GCACACTTAG AAAATCAACA AAGATTCTTT GAGACAACAG GGAAACCAGT TTTCCCTAAG 
CCTATGCCAA AGCAAAACCT GCAGATATCA GAAGACCCCA GACTGTCCAA GCCTGGAGGC 
ATGCACTATT CTGCAAACAG TGATATGGCA GGGCGGAATA ATGGTGAAAG CTCAACCCCC 
ATTGAACACC ACATTAATGA AGGTCTGGAT GGCCCTAACT CAGTGAACCA TCAAGCATTG 
CTAGAACAAC AGCGACTAGG CACATTTGCA GAGTATGAGG CCAAATGGAA CACCCAGAGG 
AAGACTACAG AACCAAGAGT ATCTGGCCGA TACCACTCTG CTGATAACAT CCTCGATACA 
GGAAAAGAGC GACGGAGTAA CCCCACCTGT GTGCATGAAA GATCTAGATC CTCACCTTCT 
GCTGACTTTT ATGGGCAGAA ACTTCCACCA CAAGAAAAGA AGTCAGCTGA CTGTTTCAAA 
TCTGAAAAGA GTCCTTGCCA ACAGGACAAG AATAGTACAA GTCAAAATTT CTACCCATCT 
GTTTCTTTGG AGCACAGCCA CTGGTTCTAT ACACCCAGCA GGTTAAATGA AAAAGGATAT 
AGTGAAATCT TATGCAAGGA GAAACCAGAA ACACCTCCTG AAGAACCTGA GAAAAAGACT 
GTAGATCCTC CCTCCAGCCA TCATAAGCCT GCACTACATT TCCCTGTCCA TAGCACTCGC 
AGTGAACCAT CAGCCCTCTC CAAAAACAAG AGCTCATTTC TCTTGCCCCA TCTGGAGAAG 
TACAAATGCC CTGAGGGAAC ACCTCCTCCT CTTAGCAATA TTCAGGAGGT CCAGCCTGGA 
TCACAAGGAG GAGCTCTGGC CCCAATTTCC CCAGTCAACA ATCAAAGCTC CGCCCCAGTT 
TCAGCCCCCA TTCCTTGGAA GAGTCCAGAG TACAGCAAAG GGCCTGTCGG GGAGGAGGAG 
CAACACCAAG AGCTTGTGCC TCCTCCCTTT CCACCTCCTC CCCCTCCCGT TGTCCTGCCC 
ACCCAACAAA CCGCTGGCCC GACCCAGCCC ACAATGGATG GGCAGCGTTC GCCCTCGCCC 
CAGTTCGCTC CCCAGAGGCT GACTGACAAG CCCCCTGTCT CCGTCTCCTC ACAGGATGAA 
GCTCCTGGAA GGATGGATCA GATTAAAGAT GAGAACACAT CTGTGAAGAA AGTGCCCATT 
AAGATAGTCC ATTCAGAGAG TGACACGGAA AAAGAAAGTC GGCAATATCT AGACCTGCCC 
AGCGAGACGC CTGTCAACTC GCAGGGACTT GTGGGAGCTC ATCTTCAAAG TTTGGGGAAC 
CCGGATCAGT CATACTCTTT GTTTTGTACA TACACCAGAC AAAAAGATCA GGTTCCTGGT 
CTTAGAGACC CAGACATGGG CCCTCTAAAA GACCAGGGGC CACAAAGCAA TATAAACCCA 
GTGTCTTATG TGGAACCTGG CCTTCAGACA GGTCCACCAT TGGATCAGAG TACCAATGGA 
GTGTCTTTGC ATCCTTCACA TTCAGAGGAT GATAAAAGAA AGGAGCTGGC TAGAGACATA 
ATGGACAAAG ACAAGTCCCT CGCTGACATT TTAGACCAGA GTAAGATGAA GACCACTATG 
GATCTGATGG AGGGGATTTT CCCTCAAGGA GATCAGCTAC TGGAGGAGGC CCAACAGCGT 
AGGAAGGCTC AACCTAAACC ACTCTCCCCT CGAAACTCTG TTGAGAAGAA AGAGGAAGAC 
AGCCTGGTGG CGGCCGCTGC TTTAGTGAGC AACTCAACCT ACTACAGCAC ATCTGCACCC 
AAAGCAGAGC TGCTGATCAA GATGAAGGAC ATGCAGGACC AGAGCGTTGA ACACAACTCA 
GAAGAGGAGC TGGAGGAGGA CAATGAAAGT GACCTCGCCT GCAAGAAGCA CGAGTTGATT 
GAAAGTCTCA GTAAGAAGTT GCAGGTCTTA AAAGAAGCTC AGGAGAGCCT GCAGGAGGAC 
GTGCAGGACA ACAACGCTCT TGGAGAAGAA GTGGAGGCCA TCGTACAGGG CGTCTGCAAG 
CCTAATGAAC TCGACAAATT CCGCATGTTC GTCGGGGATC TTGATAAAGT GGTCAACCTT 
CTGCTGTCTC TGTCAGGACG TTTGGCCAGG GTAGAGAATG CCCTGAACAG CCTGGAGGAA 
GATGCTTCGC TGGAGGAAAG GCGCACTCTG ACAGAGAAAC GCAAACTGCT GATTCGTCAG 
CACGAGGATG CTAAGGAGCT GAAGGAGAAC CTGGACAGGA GAGAGCGTGT GGTCTACGAC 
ATTCTGGCCA GTTACCTGCC TGAGGAGAGT ATGGCTGACT ACGAGCACTT TGTCAAGATG 
AAATCCGCTC TTATTATCGA GCAACGCAAG CTGGAGGACA AGATCAAACT GGGAGATGAG 
CAGCTCAAGT GTCTGATGGA CAGTCTCCCA ATGGACCAGA GAATAATCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356636.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212125.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa59475
Clone ID Related Accession (Same CDS sequence)	XM_017356636.1 , XM_017356636.2
Accession Version	XM_017356636.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAGAAATG AACCCATGAG CAGACCTCAC TCCTGGCATG CCACCAAGTT CAACGAGACC 
CATTCAGAAG CCAAAACCCA GTCCACACCC TCACCAGTTT GGCAAACAAG ATATGATGCA 
AGTTCATTCT CTTCTGAACT CTCAACTGGC TGGGATCAAA CAAATCTACG GAGAGTATCC 
GATCAGTTCA GTTCTTTGGG GAGTATGGAC AGCCTAGAAC ATAGTTCTCA TCCCTATCCA 
CCTGGCCGTC TTTCCCCAAA CAAATCCAAC AATAATAGCA TTGAACATTT GGGTAGTGGT 
AAACGGGACT CGGCATACAG CTCTTTTTCT ACAAGTTCGG GAACCCCAGA TTACACACTG 
TCCAAAAGTA ACACGGCATC CACTGAGAAC ATGCTGTACA AAATAAATCA GTGGGATTCA 
AGTGCCAGGC ACAGCAATGG CAGGCACAGC CAAAACCTAA GCGAAGGGGT ACGACAAGAT 
GAGAGGCCTG GATACCTGCA CCACCTCTCA AGTACAGGTA ACCACGAGAT CCCAAGAGCT 
GAAGAACTGC CTGGCAACCG TCATTCAAGC TCTGGAAGAG TTAATATTGG ACCTGTCTGG 
CATGTTCCAG ATATGAAGAA AAACATGATA TCATCTACTC CACCTCCCAC TCCACCCACA 
CGCAGTGATA GCTTTGCAGC TACCAAGGTT CACGAGAAGG GTCTGATCAC AGGTACTTCA 
GAGGGCCTTG GTGGTCATTC TCAAGTAAAG CCTCAAGCGA AACCACAACA AAAGCCAGGA 
GAAACCCATG AAAGCACACA AAGGTCTAAC CAAGTCATCG AGATTTGTCT TGAGGGTCGA 
CGGGATTATA ATCTACAATC CAAAAATGAT TCCTCAAATA GCTGCATTTC ATCTGATTCT 
CATCATCAGT ATATCCAACG TACATCATCA GATAAGACAT ACTCCCTATC AACCACAGAC 
ATAAGAGATA GCCATCCATC CTATGGCCAT GCTCCATACC ATGCACAGCA GTACAGTGAC 
GAAGGCACTT TCCATGCTCA AACTAGAACA GCAATGAAAC CTCCATTCAG TGGCTATTTC 
AGTAGTATGC AGGAACTGCA CACAAACAAC CACATGCAAC TCAACAGCCA GAATCAAACT 
GGAAGACCAG CTGCATCACT GTCAGTTAAT TTAGGAGTCA ATTCCCACCA TATCTCTCAG 
GGATTAACAC AGGCTTCTTT AATGAGAGCT GATGACTCTA AAGCCTCTTC AGTCTCAGAA 
ATGTCCCACA ATGGACGGGA CAGAATGTCC ATAGGTTCTC AAGGAGGGAA TAAAGAGCGG 
TATTTCCCTC CTCAATCGCA GCACCATGAT AATAAAGAAA GAAGTGCCTC CTTTAAACAA 
AATGAAAACC AACATTATGT TTCTGCACCA AGTAATCCCT TGAATATGTC TGATTCAACA 
AAACATAATG AGCTTAGGGG ATGCCAAAAG CAGCACCATG TGCATGGCTC AGATGAGCTT 
TCTGGTAGTT ATCCACCCAG TAAGCAGCCC GAGCACAGGA GAAGTGCTGG CCATCTTCAT 
CATAAGGAAT ACTCGCAACA ACCCTTTCCA ACTAAAAGCG AATCAAAGAT ATGTCCCCAG 
AAAACACCTA TGTTGTATTC TCTGGCCCAA GGGCACAATG ACACAGATGA CTGTTCTAAT 
CAGATCAAAA GTGGAAGCGC ACAACATGAA GCCCTTGACA GTCTCAGTAG TAAACAGGTT 
AGACGGAATG ACCGATTTGC CACCACTTTG CGCAACGAGA TCCAGATGAG AAGGGCGCAT 
CTCCAAAAGA GTCAAAGTGC AGCCACTTTA GAAAGTCCAG TTGAAACTGT AGAAGAGTCT 
GCTTTGTGGA AGACCACAGG GACTGCTTCT CCCTCATCAG ATGGCTGCTT TTCCATTTCT 
TATAAAGACC ATCTTAAAGA AGCTCAGGCC AGAGTCCTCC AGGCTACATC ATTCAGGAGA 
AAAGACCTAG AGCCAGTATT ATTTGAGCAT CCAGGTACTG AAGGTCCCAC ACGAAAAGAT 
GTACCTCCTT CAAGTGTCTC AGAGGTCACA CCTAGCAAAC CCACCTCAGG GAGTAATCAT 
GTGCTTCGCA TTGGAGGCCG TAAGCGGATA TCTGCTGAAA AGAAAGTGAC GTCTTTTTCT 
GAGCCAGATA AAATTCATGA AGTAGGAGCT GATGAACGCT CCACTATGCC TGAAAATGTT 
GCACACTTAG AAAATCAACA AAGATTCTTT GAGACAACAG GGAAACCAGT TTTCCCTAAG 
CCTATGCCAA AGCAAAACCT GCAGATATCA GAAGACCCCA GACTGTCCAA GCCTGGAGGC 
ATGCACTATT CTGCAAACAG TGATATGGCA GGGCGGAATA ATGGTGAAAG CTCAACCCCC 
ATTGAACACC ACATTAATGA AGGTCTGGAT GGCCCTAACT CAGTGAACCA TCAAGCATTG 
CTAGAACAAC AGCGACTAGG CACATTTGCA GAGTATGAGG CCAAATGGAA CACCCAGAGG 
AAGACTACAG AACCAAGAGT ATCTGGCCGA TACCACTCTG CTGATAACAT CCTCGATACA 
GGAAAAGAGC GACGGAGTAA CCCCACCTGT GTGCATGAAA GATCTAGATC CTCACCTTCT 
GCTGACTTTT ATGGGCAGAA ACTTCCACCA CAAGAAAAGA AGTCAGCTGA CTGTTTCAAA 
TCTGAAAAGA GTCCTTGCCA ACAGGACAAG AATAGTACAA GTCAAAATTT CTACCCATCT 
GTTTCTTTGG AGCACAGCCA CTGGTTCTAT ACACCCAGCA GGTTAAATGA AAAAGGATAT 
AGTGAAATCT TATGCAAGGA GAAACCAGAA ACACCTCCTG AAGAACCTGA GAAAAAGACT 
GTAGATCCTC CCTCCAGCCA TCATAAGCCT GCACTACATT TCCCTGTCCA TAGCACTCGC 
AGTGAACCAT CAGCCCTCTC CAAAAACAAG AGCTCATTTC TCTTGCCCCA TCTGGAGAAG 
TACAAATGCC CTGAGGGAAC ACCTCCTCCT CTTAGCAATA TTCAGGAGGT CCAGCCTGGA 
TCACAAGGAG GAGCTCTGGC CCCAATTTCC CCAGTCAACA ATCAAAGCTC CGCCCCAGTT 
TCAGCCCCCA TTCCTTGGAA GAGTCCAGAG TACAGCAAAG GGCCTGTCGG GGAGGAGGAG 
CAACACCAAG AGCTTGTGCC TCCTCCCTTT CCACCTCCTC CCCCTCCCGT TGTCCTGCCC 
ACCCAACAAA CCGCTGGCCC GACCCAGCCC ACAATGGATG GGCAGCGTTC GCCCTCGCCC 
CAGTTCGCTC CCCAGAGGCT GACTGACAAG CCCCCTGTCT CCGTCTCCTC ACAGGATGAA 
GCTCCTGGAA GGATGGATCA GATTAAAGAT GAGAACACAT CTGTGAAGAA AGTGCCCATT 
AAGATAGTCC ATTCAGAGAG TGACACGGAA AAAGAAAGTC GGCAATATCT AGACCTGCCC 
AGCGAGACGC CTGTCAACTC GCAGGGACTT GTGGGAGCTC ATCTTCAAAG TTTGGGGAAC 
CCGGATCAGT CATACTCTTT GTTTTGTACA TACACCAGAC AAAAAGATCA GGTTCCTGGT 
CTTAGAGACC CAGACATGGG CCCTCTAAAA GACCAGGGGC CACAAAGCAA TATAAACCCA 
GTGTCTTATG TGGAACCTGG CCTTCAGACA GGTCCACCAT TGGATCAGAG TACCAATGGA 
GTGTCTTTGC ATCCTTCACA TTCAGAGGAT GATAAAAGAA AGGAGCTGGC TAGAGACATA 
ATGGACAAAG ACAAGTCCCT CGCTGACATT TTAGACCAGA GTAAGATGAA GACCACTATG 
GATCTGATGG AGGGGATTTT CCCTCAAGGA GATCAGCTAC TGGAGGAGGC CCAACAGCGT 
AGGAAGGCTC AACCTAAACC ACTCTCCCCT CGAAACTCTG TTGAGAAGAA AGAGGAAGAC 
AGCCTGGTGG CGGCCGCTGC TTTAGTGAGC AACTCAACCT ACTACAGCAC ATCTGCACCC 
AAAGCAGAGC TGCTGATCAA GATGAAGGAC ATGCAGGACC AGAGCGTTGA ACACAACTCA 
GAAGAGGAGC TGGAGGAGGA CAATGAAAGT GACCTCGCCT GCAAGAAGCA CGAGTTGATT 
GAAAGTCTCA GTAAGAAGTT GCAGGTCTTA AAAGAAGCTC AGGAGAGCCT GCAGGAGGAC 
GTGCAGGACA ACAACGCTCT TGGAGAAGAA GTGGAGGCCA TCGTACAGGG CGTCTGCAAG 
CCTAATGAAC TCGACAAATT CCGCATGTTC GTCGGGGATC TTGATAAAGT GGTCAACCTT 
CTGCTGTCTC TGTCAGGACG TTTGGCCAGG GTAGAGAATG CCCTGAACAG CCTGGAGGAA 
GATGCTTCGC TGGAGGAAAG GCGCACTCTG ACAGAGAAAC GCAAACTGCT GATTCGTCAG 
CACGAGGATG CTAAGGAGCT GAAGGAGAAC CTGGACAGGA GAGAGCGTGT GGTCTACGAC 
ATTCTGGCCA GTTACCTGCC TGAGGAGAGT ATGGCTGACT ACGAGCACTT TGTCAAGATG 
AAATCCGCTC TTATTATCGA GCAACGCAAG CTGGAGGACA AGATCAAACT GGGAGATGAG 
CAGCTCAAGT GTCTGATGGA CAGTCTCCCA ATGGACCAGA GAATAATCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212125.1
CDS	772..5805
Translation	MDTINQYPVNPSFPEDDHRSFCSSIRSASFNEHHVNDEGWKVVEVTLSGGAPWGFTLRGGLEHGEPLLITKVEEGSKAATVRLQVGDELININNISLTGFRQEAICLVKSSYKTLSLVVKRRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356636.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356636.2

ORF Insert Sequence:

ATGGACACGA TCAACCAATA CCCGGTGAAC CCCAGTTTTC CAGAAGACGA TCACAGGAGC 
TTTTGCAGTT CAATCAGATC TGCGAGTTTT AATGAACATC ACGTTAATGA TGAGGGATGG 
AAAGTAGTGG AAGTGACCCT GTCCGGGGGA GCACCGTGGG GTTTTACCCT CAGAGGAGGA 
CTGGAGCACG GAGAACCTCT TCTTATAACC AAGGTGGAAG AGGGCAGTAA GGCTGCGACC 
GTGAGACTGC AGGTTGGAGA TGAACTCATC AACATCAACA ACATTTCTCT CACTGGCTTC 
AGACAAGAGG CCATCTGTCT AGTCAAAAGC TCCTACAAGA CTCTCAGCCT GGTGGTCAAA 
AGGAGAAATG AACCCATGAG CAGACCTCAC TCCTGGCATG CCACCAAGTT CAACGAGACC 
CATTCAGAAG CCAAAACCCA GTCCACACCC TCACCAGTTT GGCAAACAAG ATATGATGCA 
AGTTCATTCT CTTCTGAACT CTCAACTGGC TGGGATCAAA CAAATCTACG GAGAGTATCC 
GATCAGTTCA GTTCTTTGGG GAGTATGGAC AGCCTAGAAC ATAGTTCTCA TCCCTATCCA 
CCTGGCCGTC TTTCCCCAAA CAAATCCAAC AATAATAGCA TTGAACATTT GGGTAGTGGT 
AAACGGGACT CGGCATACAG CTCTTTTTCT ACAAGTTCGG GAACCCCAGA TTACACACTG 
TCCAAAAGTA ACACGGCATC CACTGAGAAC ATGCTGTACA AAATAAATCA GTGGGATTCA 
AGTGCCAGGC ACAGCAATGG CAGGCACAGC CAAAACCTAA GCGAAGGGGT ACGACAAGAT 
GAGAGGCCTG GATACCTGCA CCACCTCTCA AGTACAGGTA ACCACGAGAT CCCAAGAGCT 
GAAGAACTGC CTGGCAACCG TCATTCAAGC TCTGGAAGAG TTAATATTGG ACCTGTCTGG 
CATGTTCCAG ATATGAAGAA AAACATGATA TCATCTACTC CACCTCCCAC TCCACCCACA 
CGCAGTGATA GCTTTGCAGC TACCAAGGTT CACGAGAAGG GTCTGATCAC AGGTACTTCA 
GAGGGCCTTG GTGGTCATTC TCAAGTAAAG CCTCAAGCGA AACCACAACA AAAGCCAGGA 
GAAACCCATG AAAGCACACA AAGGTCTAAC CAAGTCATCG AGATTTGTCT TGAGGGTCGA 
CGGGATTATA ATCTACAATC CAAAAATGAT TCCTCAAATA GCTGCATTTC ATCTGATTCT 
CATCATCAGT ATATCCAACG TACATCATCA GATAAGACAT ACTCCCTATC AACCACAGAC 
ATAAGAGATA GCCATCCATC CTATGGCCAT GCTCCATACC ATGCACAGCA GTACAGTGAC 
GAAGGCACTT TCCATGCTCA AACTAGAACA GCAATGAAAC CTCCATTCAG TGGCTATTTC 
AGTAGTATGC AGGAACTGCA CACAAACAAC CACATGCAAC TCAACAGCCA GAATCAAACT 
GGAAGACCAG CTGCATCACT GTCAGTTAAT TTAGGAGTCA ATTCCCACCA TATCTCTCAG 
GGATTAACAC AGGCTTCTTT AATGAGAGCT GATGACTCTA AAGCCTCTTC AGTCTCAGAA 
ATGTCCCACA ATGGACGGGA CAGAATGTCC ATAGGTTCTC AAGGAGGGAA TAAAGAGCGG 
TATTTCCCTC CTCAATCGCA GCACCATGAT AATAAAGAAA GAAGTGCCTC CTTTAAACAA 
AATGAAAACC AACATTATGT TTCTGCACCA AGTAATCCCT TGAATATGTC TGATTCAACA 
AAACATAATG AGCTTAGGGG ATGCCAAAAG CAGCACCATG TGCATGGCTC AGATGAGCTT 
TCTGGTAGTT ATCCACCCAG TAAGCAGCCC GAGCACAGGA GAAGTGCTGG CCATCTTCAT 
CATAAGGAAT ACTCGCAACA ACCCTTTCCA ACTAAAAGCG AATCAAAGAT ATGTCCCCAG 
AAAACACCTA TGTTGTATTC TCTGGCCCAA GGGCACAATG ACACAGATGA CTGTTCTAAT 
CAGATCAAAA GTGGAAGCGC ACAACATGAA GCCCTTGACA GTCTCAGTAG TAAACAGGTT 
AGACGGAATG ACCGATTTGC CACCACTTTG CGCAACGAGA TCCAGATGAG AAGGGCGCAT 
CTCCAAAAGA GTCAAAGTGC AGCCACTTTA GAAAGTCCAG TTGAAACTGT AGAAGAGTCT 
GCTTTGTGGA AGACCACAGG GACTGCTTCT CCCTCATCAG ATGGCTGCTT TTCCATTTCT 
TATAAAGACC ATCTTAAAGA AGCTCAGGCC AGAGTCCTCC AGGCTACATC ATTCAGGAGA 
AAAGACCTAG AGCCAGTATT ATTTGAGCAT CCAGGTACTG AAGGTCCCAC ACGAAAAGAT 
GTACCTCCTT CAAGTGTCTC AGAGGTCACA CCTAGCAAAC CCACCTCAGG GAGTAATCAT 
GTGCTTCGCA TTGGAGGCCG TAAGCGGATA TCTGCTGAAA AGAAAGTGAC GTCTTTTTCT 
GAGCCAGATA AAATTCATGA AGTAGGAGCT GATGAACGCT CCACTATGCC TGAAAATGTT 
GCACACTTAG AAAATCAACA AAGATTCTTT GAGACAACAG GGAAACCAGT TTTCCCTAAG 
CCTATGCCAA AGCAAAACCT GCAGATATCA GAAGACCCCA GACTGTCCAA GCCTGGAGGC 
ATGCACTATT CTGCAAACAG TGATATGGCA GGGCGGAATA ATGGTGAAAG CTCAACCCCC 
ATTGAACACC ACATTAATGA AGGTCTGGAT GGCCCTAACT CAGTGAACCA TCAAGCATTG 
CTAGAACAAC AGCGACTAGG CACATTTGCA GAGTATGAGG CCAAATGGAA CACCCAGAGG 
AAGACTACAG AACCAAGAGT ATCTGGCCGA TACCACTCTG CTGATAACAT CCTCGATACA 
GGAAAAGAGC GACGGAGTAA CCCCACCTGT GTGCATGAAA GATCTAGATC CTCACCTTCT 
GCTGACTTTT ATGGGCAGAA ACTTCCACCA CAAGAAAAGA AGTCAGCTGA CTGTTTCAAA 
TCTGAAAAGA GTCCTTGCCA ACAGGACAAG AATAGTACAA GTCAAAATTT CTACCCATCT 
GTTTCTTTGG AGCACAGCCA CTGGTTCTAT ACACCCAGCA GGTTAAATGA AAAAGGATAT 
AGTGAAATCT TATGCAAGGA GAAACCAGAA ACACCTCCTG AAGAACCTGA GAAAAAGACT 
GTAGATCCTC CCTCCAGCCA TCATAAGCCT GCACTACATT TCCCTGTCCA TAGCACTCGC 
AGTGAACCAT CAGCCCTCTC CAAAAACAAG AGCTCATTTC TCTTGCCCCA TCTGGAGAAG 
TACAAATGCC CTGAGGGAAC ACCTCCTCCT CTTAGCAATA TTCAGGAGGT CCAGCCTGGA 
TCACAAGGAG GAGCTCTGGC CCCAATTTCC CCAGTCAACA ATCAAAGCTC CGCCCCAGTT 
TCAGCCCCCA TTCCTTGGAA GAGTCCAGAG TACAGCAAAG GGCCTGTCGG GGAGGAGGAG 
CAACACCAAG AGCTTGTGCC TCCTCCCTTT CCACCTCCTC CCCCTCCCGT TGTCCTGCCC 
ACCCAACAAA CCGCTGGCCC GACCCAGCCC ACAATGGATG GGCAGCGTTC GCCCTCGCCC 
CAGTTCGCTC CCCAGAGGCT GACTGACAAG CCCCCTGTCT CCGTCTCCTC ACAGGATGAA 
GCTCCTGGAA GGATGGATCA GATTAAAGAT GAGAACACAT CTGTGAAGAA AGTGCCCATT 
AAGATAGTCC ATTCAGAGAG TGACACGGAA AAAGAAAGTC GGCAATATCT AGACCTGCCC 
AGCGAGACGC CTGTCAACTC GCAGGGACTT GTGGGAGCTC ATCTTCAAAG TTTGGGGAAC 
CCGGATCAGT CATACTCTTT GTTTTGTACA TACACCAGAC AAAAAGATCA GGTTCCTGGT 
CTTAGAGACC CAGACATGGG CCCTCTAAAA GACCAGGGGC CACAAAGCAA TATAAACCCA 
GTGTCTTATG TGGAACCTGG CCTTCAGACA GGTCCACCAT TGGATCAGAG TACCAATGGA 
GTGTCTTTGC ATCCTTCACA TTCAGAGGAT GATAAAAGAA AGGAGCTGGC TAGAGACATA 
ATGGACAAAG ACAAGTCCCT CGCTGACATT TTAGACCAGA GTAAGATGAA GACCACTATG 
GATCTGATGG AGGGGATTTT CCCTCAAGGA GATCAGCTAC TGGAGGAGGC CCAACAGCGT 
AGGAAGGCTC AACCTAAACC ACTCTCCCCT CGAAACTCTG TTGAGAAGAA AGAGGAAGAC 
AGCCTGGTGG CGGCCGCTGC TTTAGTGAGC AACTCAACCT ACTACAGCAC ATCTGCACCC 
AAAGCAGAGC TGCTGATCAA GATGAAGGAC ATGCAGGACC AGAGCGTTGA ACACAACTCA 
GAAGAGGAGC TGGAGGAGGA CAATGAAAGT GACCTCGCCT GCAAGAAGCA CGAGTTGATT 
GAAAGTCTCA GTAAGAAGTT GCAGGTCTTA AAAGAAGCTC AGGAGAGCCT GCAGGAGGAC 
GTGCAGGACA ACAACGCTCT TGGAGAAGAA GTGGAGGCCA TCGTACAGGG CGTCTGCAAG 
CCTAATGAAC TCGACAAATT CCGCATGTTC GTCGGGGATC TTGATAAAGT GGTCAACCTT 
CTGCTGTCTC TGTCAGGACG TTTGGCCAGG GTAGAGAATG CCCTGAACAG CCTGGAGGAA 
GATGCTTCGC TGGAGGAAAG GCGCACTCTG ACAGAGAAAC GCAAACTGCT GATTCGTCAG 
CACGAGGATG CTAAGGAGCT GAAGGAGAAC CTGGACAGGA GAGAGCGTGT GGTCTACGAC 
ATTCTGGCCA GTTACCTGCC TGAGGAGAGT ATGGCTGACT ACGAGCACTT TGTCAAGATG 
AAATCCGCTC TTATTATCGA GCAACGCAAG CTGGAGGACA AGATCAAACT GGGAGATGAG 
CAGCTCAAGT GTCTGATGGA CAGTCTCCCA ATGGACCAGA GAATAATCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356637.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212126.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X5, mRNA.

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CloneID	ODa59476
Clone ID Related Accession (Same CDS sequence)	XM_017356637.2 , XM_017356637.1 , XM_017356638.1 , XM_017356638.2 , XM_021477154.1
Accession Version	XM_017356637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGG TGGATATTGT AGCGCAGAAA ATGCCATCAG AGAGCGACGT GCATATGGCC 
CGGAGCTTTC TCACGAAGAT TTTACGAAGT TCCATGAGAA AGAATCGGTT TAAAGGGAGA 
AATGAACCCA TGAGCAGACC TCACTCCTGG CATGCCACCA AGTTCAACGA GACCCATTCA 
GAAGCCAAAA CCCAGTCCAC ACCCTCACCA GTTTGGCAAA CAAGATATGA TGCAAGTTCA 
TTCTCTTCTG AACTCTCAAC TGGCTGGGAT CAAACAAATC TACGGAGAGT ATCCGATCAG 
TTCAGTTCTT TGGGGAGTAT GGACAGCCTA GAACATAGTT CTCATCCCTA TCCACCTGGC 
CGTCTTTCCC CAAACAAATC CAACAATAAT AGCATTGAAC ATTTGGGTAG TGGTAAACGG 
GACTCGGCAT ACAGCTCTTT TTCTACAAGT TCGGGAACCC CAGATTACAC ACTGTCCAAA 
AGTAACACGG CATCCACTGA GAACATGCTG TACAAAATAA ATCAGTGGGA TTCAAGTGCC 
AGGCACAGCA ATGGCAGGCA CAGCCAAAAC CTAAGCGAAG GGGTACGACA AGATGAGAGG 
CCTGGATACC TGCACCACCT CTCAAGTACA GGTAACCACG AGATCCCAAG AGCTGAAGAA 
CTGCCTGGCA ACCGTCATTC AAGCTCTGGA AGAGTTAATA TTGGACCTGT CTGGCATGTT 
CCAGATATGA AGAAAAACAT GATATCATCT ACTCCACCTC CCACTCCACC CACACGCAGT 
GATAGCTTTG CAGCTACCAA GGTTCACGAG AAGGGTCTGA TCACAGGTAC TTCAGAGGGC 
CTTGGTGGTC ATTCTCAAGT AAAGCCTCAA GCGAAACCAC AACAAAAGCC AGGAGAAACC 
CATGAAAGCA CACAAAGGTC TAACCAAGTC ATCGAGATTT GTCTTGAGGG TCGACGGGAT 
TATAATCTAC AATCCAAAAA TGATTCCTCA AATAGCTGCA TTTCATCTGA TTCTCATCAT 
CAGTATATCC AACGTACATC ATCAGATAAG ACATACTCCC TATCAACCAC AGACATAAGA 
GATAGCCATC CATCCTATGG CCATGCTCCA TACCATGCAC AGCAGTACAG TGACGAAGGC 
ACTTTCCATG CTCAAACTAG AACAGCAATG AAACCTCCAT TCAGTGGCTA TTTCAGTAGT 
ATGCAGGAAC TGCACACAAA CAACCACATG CAACTCAACA GCCAGAATCA AACTGGAAGA 
CCAGCTGCAT CACTGTCAGT TAATTTAGGA GTCAATTCCC ACCATATCTC TCAGGGATTA 
ACACAGGCTT CTTTAATGAG AGCTGATGAC TCTAAAGCCT CTTCAGTCTC AGAAATGTCC 
CACAATGGAC GGGACAGAAT GTCCATAGGT TCTCAAGGAG GGAATAAAGA GCGGTATTTC 
CCTCCTCAAT CGCAGCACCA TGATAATAAA GAAAGAAGTG CCTCCTTTAA ACAAAATGAA 
AACCAACATT ATGTTTCTGC ACCAAGTAAT CCCTTGAATA TGTCTGATTC AACAAAACAT 
AATGAGCTTA GGGGATGCCA AAAGCAGCAC CATGTGCATG GCTCAGATGA GCTTTCTGGT 
AGTTATCCAC CCAGTAAGCA GCCCGAGCAC AGGAGAAGTG CTGGCCATCT TCATCATAAG 
GAATACTCGC AACAACCCTT TCCAACTAAA AGCGAATCAA AGATATGTCC CCAGAAAACA 
CCTATGTTGT ATTCTCTGGC CCAAGGGCAC AATGACACAG ATGACTGTTC TAATCAGATC 
AAAAGTGGAA GCGCACAACA TGAAGCCCTT GACAGTCTCA GTAGTAAACA GGTTAGACGG 
AATGACCGAT TTGCCACCAC TTTGCGCAAC GAGATCCAGA TGAGAAGGGC GCATCTCCAA 
AAGAGTCAAA GTGCAGCCAC TTTAGAAAGT CCAGTTGAAA CTGTAGAAGA GTCTGCTTTG 
TGGAAGACCA CAGGGACTGC TTCTCCCTCA TCAGATGGCT GCTTTTCCAT TTCTTATAAA 
GACCATCTTA AAGAAGCTCA GGCCAGAGTC CTCCAGGCTA CATCATTCAG GAGAAAAGAC 
CTAGAGCCAG TATTATTTGA GCATCCAGGT ACTGAAGGTC CCACACGAAA AGATGTACCT 
CCTTCAAGTG TCTCAGAGGT CACACCTAGC AAACCCACCT CAGGGAGTAA TCATGTGCTT 
CGCATTGGAG GCCGTAAGCG GATATCTGCT GAAAAGAAAG TGACGTCTTT TTCTGAGCCA 
GATAAAATTC ATGAAGTAGG AGCTGATGAA CGCTCCACTA TGCCTGAAAA TGTTGCACAC 
TTAGAAAATC AACAAAGATT CTTTGAGACA ACAGGGAAAC CAGTTTTCCC TAAGCCTATG 
CCAAAGCAAA ACCTGCAGAT ATCAGAAGAC CCCAGACTGT CCAAGCCTGG AGGCATGCAC 
TATTCTGCAA ACAGTGATAT GGCAGGGCGG AATAATGGTG AAAGCTCAAC CCCCATTGAA 
CACCACATTA ATGAAGGTCT GGATGGCCCT AACTCAGTGA ACCATCAAGC ATTGCTAGAA 
CAACAGCGAC TAGGCACATT TGCAGAGTAT GAGGCCAAAT GGAACACCCA GAGGAAGACT 
ACAGAACCAA GAGTATCTGG CCGATACCAC TCTGCTGATA ACATCCTCGA TACAGGAAAA 
GAGCGACGGA GTAACCCCAC CTGTGTGCAT GAAAGATCTA GATCCTCACC TTCTGCTGAC 
TTTTATGGGC AGAAACTTCC ACCACAAGAA AAGAAGTCAG CTGACTGTTT CAAATCTGAA 
AAGAGTCCTT GCCAACAGGA CAAGAATAGT ACAAGTCAAA ATTTCTACCC ATCTGTTTCT 
TTGGAGCACA GCCACTGGTT CTATACACCC AGCAGGTTAA ATGAAAAAGG ATATAGTGAA 
ATCTTATGCA AGGAGAAACC AGAAACACCT CCTGAAGAAC CTGAGAAAAA GACTGTAGAT 
CCTCCCTCCA GCCATCATAA GCCTGCACTA CATTTCCCTG TCCATAGCAC TCGCAGTGAA 
CCATCAGCCC TCTCCAAAAA CAAGAGCTCA TTTCTCTTGC CCCATCTGGA GAAGTACAAA 
TGCCCTGAGG GAACACCTCC TCCTCTTAGC AATATTCAGG AGGTCCAGCC TGGATCACAA 
GGAGGAGCTC TGGCCCCAAT TTCCCCAGTC AACAATCAAA GCTCCGCCCC AGTTTCAGCC 
CCCATTCCTT GGAAGAGTCC AGAGTACAGC AAAGGGCCTG TCGGGGAGGA GGAGCAACAC 
CAAGAGCTTG TGCCTCCTCC CTTTCCACCT CCTCCCCCTC CCGTTGTCCT GCCCACCCAA 
CAAACCGCTG GCCCGACCCA GCCCACAATG GATGGGCAGC GTTCGCCCTC GCCCCAGTTC 
GCTCCCCAGA GGCTGACTGA CAAGCCCCCT GTCTCCGTCT CCTCACAGGA TGAAGCTCCT 
GGAAGGATGG ATCAGATTAA AGATGAGAAC ACATCTGTGA AGAAAGTGCC CATTAAGATA 
GTCCATTCAG AGAGTGACAC GGAAAAAGAA AGTCGGCAAT ATCTAGACCT GCCCAGCGAG 
ACGCCTGTCA ACTCGCAGGG ACTTGTGGGA GCTCATCTTC AAAGTTTGGG GAACCCGGAT 
CAGTCATACT CTTTGTTTTG TACATACACC AGACAAAAAG ATCAGGTTCC TGGTCTTAGA 
GACCCAGACA TGGGCCCTCT AAAAGACCAG GGGCCACAAA GCAATATAAA CCCAGTGTCT 
TATGTGGAAC CTGGCCTTCA GACAGGTCCA CCATTGGATC AGAGTACCAA TGGAGTGTCT 
TTGCATCCTT CACATTCAGA GGATGATAAA AGAAAGGAGC TGGCTAGAGA CATAATGGAC 
AAAGACAAGT CCCTCGCTGA CATTTTAGAC CAGAGTAAGA TGAAGACCAC TATGGATCTG 
ATGGAGGGGA TTTTCCCTCA AGGAGATCAG CTACTGGAGG AGGCCCAACA GCGTAGGAAG 
GCTCAACCTA AACCACTCTC CCCTCGAAAC TCTGTTGAGA AGAAAGAGGA AGACAGCCTG 
GTGGCGGCCG CTGCTTTAGT GAGCAACTCA ACCTACTACA GCACATCTGC ACCCAAAGCA 
GAGCTGCTGA TCAAGATGAA GGACATGCAG GACCAGAGCG TTGAACACAA CTCAGAAGAG 
GAGCTGGAGG AGGACAATGA AAGTGACCTC GCCTGCAAGA AGCACGAGTT GATTGAAAGT 
CTCAGTAAGA AGTTGCAGGT CTTAAAAGAA GCTCAGGAGA GCCTGCAGGA GGACGTGCAG 
GACAACAACG CTCTTGGAGA AGAAGTGGAG GCCATCGTAC AGGGCGTCTG CAAGCCTAAT 
GAACTCGACA AATTCCGCAT GTTCGTCGGG GATCTTGATA AAGTGGTCAA CCTTCTGCTG 
TCTCTGTCAG GACGTTTGGC CAGGGTAGAG AATGCCCTGA ACAGCCTGGA GGAAGATGCT 
TCGCTGGAGG AAAGGCGCAC TCTGACAGAG AAACGCAAAC TGCTGATTCG TCAGCACGAG 
GATGCTAAGG AGCTGAAGGA GAACCTGGAC AGGAGAGAGC GTGTGGTCTA CGACATTCTG 
GCCAGTTACC TGCCTGAGGA GAGTATGGCT GACTACGAGC ACTTTGTCAA GATGAAATCC 
GCTCTTATTA TCGAGCAACG CAAGCTGGAG GACAAGATCA AACTGGGAGA TGAGCAGCTC 
AAGTGTCTGA TGGACAGTCT CCCAATGGAC CAGAGAATAA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212126.1
CDS	626..5413
Translation	MKMVDIVAQKMPSESDVHMARSFLTKILRSSMRKNRFKGRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356637.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356637.2

ORF Insert Sequence:

ATGAAAATGG TGGATATTGT AGCGCAGAAA ATGCCATCAG AGAGCGACGT GCATATGGCC 
CGGAGCTTTC TCACGAAGAT TTTACGAAGT TCCATGAGAA AGAATCGGTT TAAAGGGAGA 
AATGAACCCA TGAGCAGACC TCACTCCTGG CATGCCACCA AGTTCAACGA GACCCATTCA 
GAAGCCAAAA CCCAGTCCAC ACCCTCACCA GTTTGGCAAA CAAGATATGA TGCAAGTTCA 
TTCTCTTCTG AACTCTCAAC TGGCTGGGAT CAAACAAATC TACGGAGAGT ATCCGATCAG 
TTCAGTTCTT TGGGGAGTAT GGACAGCCTA GAACATAGTT CTCATCCCTA TCCACCTGGC 
CGTCTTTCCC CAAACAAATC CAACAATAAT AGCATTGAAC ATTTGGGTAG TGGTAAACGG 
GACTCGGCAT ACAGCTCTTT TTCTACAAGT TCGGGAACCC CAGATTACAC ACTGTCCAAA 
AGTAACACGG CATCCACTGA GAACATGCTG TACAAAATAA ATCAGTGGGA TTCAAGTGCC 
AGGCACAGCA ATGGCAGGCA CAGCCAAAAC CTAAGCGAAG GGGTACGACA AGATGAGAGG 
CCTGGATACC TGCACCACCT CTCAAGTACA GGTAACCACG AGATCCCAAG AGCTGAAGAA 
CTGCCTGGCA ACCGTCATTC AAGCTCTGGA AGAGTTAATA TTGGACCTGT CTGGCATGTT 
CCAGATATGA AGAAAAACAT GATATCATCT ACTCCACCTC CCACTCCACC CACACGCAGT 
GATAGCTTTG CAGCTACCAA GGTTCACGAG AAGGGTCTGA TCACAGGTAC TTCAGAGGGC 
CTTGGTGGTC ATTCTCAAGT AAAGCCTCAA GCGAAACCAC AACAAAAGCC AGGAGAAACC 
CATGAAAGCA CACAAAGGTC TAACCAAGTC ATCGAGATTT GTCTTGAGGG TCGACGGGAT 
TATAATCTAC AATCCAAAAA TGATTCCTCA AATAGCTGCA TTTCATCTGA TTCTCATCAT 
CAGTATATCC AACGTACATC ATCAGATAAG ACATACTCCC TATCAACCAC AGACATAAGA 
GATAGCCATC CATCCTATGG CCATGCTCCA TACCATGCAC AGCAGTACAG TGACGAAGGC 
ACTTTCCATG CTCAAACTAG AACAGCAATG AAACCTCCAT TCAGTGGCTA TTTCAGTAGT 
ATGCAGGAAC TGCACACAAA CAACCACATG CAACTCAACA GCCAGAATCA AACTGGAAGA 
CCAGCTGCAT CACTGTCAGT TAATTTAGGA GTCAATTCCC ACCATATCTC TCAGGGATTA 
ACACAGGCTT CTTTAATGAG AGCTGATGAC TCTAAAGCCT CTTCAGTCTC AGAAATGTCC 
CACAATGGAC GGGACAGAAT GTCCATAGGT TCTCAAGGAG GGAATAAAGA GCGGTATTTC 
CCTCCTCAAT CGCAGCACCA TGATAATAAA GAAAGAAGTG CCTCCTTTAA ACAAAATGAA 
AACCAACATT ATGTTTCTGC ACCAAGTAAT CCCTTGAATA TGTCTGATTC AACAAAACAT 
AATGAGCTTA GGGGATGCCA AAAGCAGCAC CATGTGCATG GCTCAGATGA GCTTTCTGGT 
AGTTATCCAC CCAGTAAGCA GCCCGAGCAC AGGAGAAGTG CTGGCCATCT TCATCATAAG 
GAATACTCGC AACAACCCTT TCCAACTAAA AGCGAATCAA AGATATGTCC CCAGAAAACA 
CCTATGTTGT ATTCTCTGGC CCAAGGGCAC AATGACACAG ATGACTGTTC TAATCAGATC 
AAAAGTGGAA GCGCACAACA TGAAGCCCTT GACAGTCTCA GTAGTAAACA GGTTAGACGG 
AATGACCGAT TTGCCACCAC TTTGCGCAAC GAGATCCAGA TGAGAAGGGC GCATCTCCAA 
AAGAGTCAAA GTGCAGCCAC TTTAGAAAGT CCAGTTGAAA CTGTAGAAGA GTCTGCTTTG 
TGGAAGACCA CAGGGACTGC TTCTCCCTCA TCAGATGGCT GCTTTTCCAT TTCTTATAAA 
GACCATCTTA AAGAAGCTCA GGCCAGAGTC CTCCAGGCTA CATCATTCAG GAGAAAAGAC 
CTAGAGCCAG TATTATTTGA GCATCCAGGT ACTGAAGGTC CCACACGAAA AGATGTACCT 
CCTTCAAGTG TCTCAGAGGT CACACCTAGC AAACCCACCT CAGGGAGTAA TCATGTGCTT 
CGCATTGGAG GCCGTAAGCG GATATCTGCT GAAAAGAAAG TGACGTCTTT TTCTGAGCCA 
GATAAAATTC ATGAAGTAGG AGCTGATGAA CGCTCCACTA TGCCTGAAAA TGTTGCACAC 
TTAGAAAATC AACAAAGATT CTTTGAGACA ACAGGGAAAC CAGTTTTCCC TAAGCCTATG 
CCAAAGCAAA ACCTGCAGAT ATCAGAAGAC CCCAGACTGT CCAAGCCTGG AGGCATGCAC 
TATTCTGCAA ACAGTGATAT GGCAGGGCGG AATAATGGTG AAAGCTCAAC CCCCATTGAA 
CACCACATTA ATGAAGGTCT GGATGGCCCT AACTCAGTGA ACCATCAAGC ATTGCTAGAA 
CAACAGCGAC TAGGCACATT TGCAGAGTAT GAGGCCAAAT GGAACACCCA GAGGAAGACT 
ACAGAACCAA GAGTATCTGG CCGATACCAC TCTGCTGATA ACATCCTCGA TACAGGAAAA 
GAGCGACGGA GTAACCCCAC CTGTGTGCAT GAAAGATCTA GATCCTCACC TTCTGCTGAC 
TTTTATGGGC AGAAACTTCC ACCACAAGAA AAGAAGTCAG CTGACTGTTT CAAATCTGAA 
AAGAGTCCTT GCCAACAGGA CAAGAATAGT ACAAGTCAAA ATTTCTACCC ATCTGTTTCT 
TTGGAGCACA GCCACTGGTT CTATACACCC AGCAGGTTAA ATGAAAAAGG ATATAGTGAA 
ATCTTATGCA AGGAGAAACC AGAAACACCT CCTGAAGAAC CTGAGAAAAA GACTGTAGAT 
CCTCCCTCCA GCCATCATAA GCCTGCACTA CATTTCCCTG TCCATAGCAC TCGCAGTGAA 
CCATCAGCCC TCTCCAAAAA CAAGAGCTCA TTTCTCTTGC CCCATCTGGA GAAGTACAAA 
TGCCCTGAGG GAACACCTCC TCCTCTTAGC AATATTCAGG AGGTCCAGCC TGGATCACAA 
GGAGGAGCTC TGGCCCCAAT TTCCCCAGTC AACAATCAAA GCTCCGCCCC AGTTTCAGCC 
CCCATTCCTT GGAAGAGTCC AGAGTACAGC AAAGGGCCTG TCGGGGAGGA GGAGCAACAC 
CAAGAGCTTG TGCCTCCTCC CTTTCCACCT CCTCCCCCTC CCGTTGTCCT GCCCACCCAA 
CAAACCGCTG GCCCGACCCA GCCCACAATG GATGGGCAGC GTTCGCCCTC GCCCCAGTTC 
GCTCCCCAGA GGCTGACTGA CAAGCCCCCT GTCTCCGTCT CCTCACAGGA TGAAGCTCCT 
GGAAGGATGG ATCAGATTAA AGATGAGAAC ACATCTGTGA AGAAAGTGCC CATTAAGATA 
GTCCATTCAG AGAGTGACAC GGAAAAAGAA AGTCGGCAAT ATCTAGACCT GCCCAGCGAG 
ACGCCTGTCA ACTCGCAGGG ACTTGTGGGA GCTCATCTTC AAAGTTTGGG GAACCCGGAT 
CAGTCATACT CTTTGTTTTG TACATACACC AGACAAAAAG ATCAGGTTCC TGGTCTTAGA 
GACCCAGACA TGGGCCCTCT AAAAGACCAG GGGCCACAAA GCAATATAAA CCCAGTGTCT 
TATGTGGAAC CTGGCCTTCA GACAGGTCCA CCATTGGATC AGAGTACCAA TGGAGTGTCT 
TTGCATCCTT CACATTCAGA GGATGATAAA AGAAAGGAGC TGGCTAGAGA CATAATGGAC 
AAAGACAAGT CCCTCGCTGA CATTTTAGAC CAGAGTAAGA TGAAGACCAC TATGGATCTG 
ATGGAGGGGA TTTTCCCTCA AGGAGATCAG CTACTGGAGG AGGCCCAACA GCGTAGGAAG 
GCTCAACCTA AACCACTCTC CCCTCGAAAC TCTGTTGAGA AGAAAGAGGA AGACAGCCTG 
GTGGCGGCCG CTGCTTTAGT GAGCAACTCA ACCTACTACA GCACATCTGC ACCCAAAGCA 
GAGCTGCTGA TCAAGATGAA GGACATGCAG GACCAGAGCG TTGAACACAA CTCAGAAGAG 
GAGCTGGAGG AGGACAATGA AAGTGACCTC GCCTGCAAGA AGCACGAGTT GATTGAAAGT 
CTCAGTAAGA AGTTGCAGGT CTTAAAAGAA GCTCAGGAGA GCCTGCAGGA GGACGTGCAG 
GACAACAACG CTCTTGGAGA AGAAGTGGAG GCCATCGTAC AGGGCGTCTG CAAGCCTAAT 
GAACTCGACA AATTCCGCAT GTTCGTCGGG GATCTTGATA AAGTGGTCAA CCTTCTGCTG 
TCTCTGTCAG GACGTTTGGC CAGGGTAGAG AATGCCCTGA ACAGCCTGGA GGAAGATGCT 
TCGCTGGAGG AAAGGCGCAC TCTGACAGAG AAACGCAAAC TGCTGATTCG TCAGCACGAG 
GATGCTAAGG AGCTGAAGGA GAACCTGGAC AGGAGAGAGC GTGTGGTCTA CGACATTCTG 
GCCAGTTACC TGCCTGAGGA GAGTATGGCT GACTACGAGC ACTTTGTCAA GATGAAATCC 
GCTCTTATTA TCGAGCAACG CAAGCTGGAG GACAAGATCA AACTGGGAGA TGAGCAGCTC 
AAGTGTCTGA TGGACAGTCT CCCAATGGAC CAGAGAATAA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356637.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212126.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X6, mRNA.

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CloneID	ODa59476
Clone ID Related Accession (Same CDS sequence)	XM_017356637.2 , XM_017356637.1 , XM_017356638.1 , XM_017356638.2 , XM_021477154.1
Accession Version	XM_017356637.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom2 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGG TGGATATTGT AGCGCAGAAA ATGCCATCAG AGAGCGACGT GCATATGGCC 
CGGAGCTTTC TCACGAAGAT TTTACGAAGT TCCATGAGAA AGAATCGGTT TAAAGGGAGA 
AATGAACCCA TGAGCAGACC TCACTCCTGG CATGCCACCA AGTTCAACGA GACCCATTCA 
GAAGCCAAAA CCCAGTCCAC ACCCTCACCA GTTTGGCAAA CAAGATATGA TGCAAGTTCA 
TTCTCTTCTG AACTCTCAAC TGGCTGGGAT CAAACAAATC TACGGAGAGT ATCCGATCAG 
TTCAGTTCTT TGGGGAGTAT GGACAGCCTA GAACATAGTT CTCATCCCTA TCCACCTGGC 
CGTCTTTCCC CAAACAAATC CAACAATAAT AGCATTGAAC ATTTGGGTAG TGGTAAACGG 
GACTCGGCAT ACAGCTCTTT TTCTACAAGT TCGGGAACCC CAGATTACAC ACTGTCCAAA 
AGTAACACGG CATCCACTGA GAACATGCTG TACAAAATAA ATCAGTGGGA TTCAAGTGCC 
AGGCACAGCA ATGGCAGGCA CAGCCAAAAC CTAAGCGAAG GGGTACGACA AGATGAGAGG 
CCTGGATACC TGCACCACCT CTCAAGTACA GGTAACCACG AGATCCCAAG AGCTGAAGAA 
CTGCCTGGCA ACCGTCATTC AAGCTCTGGA AGAGTTAATA TTGGACCTGT CTGGCATGTT 
CCAGATATGA AGAAAAACAT GATATCATCT ACTCCACCTC CCACTCCACC CACACGCAGT 
GATAGCTTTG CAGCTACCAA GGTTCACGAG AAGGGTCTGA TCACAGGTAC TTCAGAGGGC 
CTTGGTGGTC ATTCTCAAGT AAAGCCTCAA GCGAAACCAC AACAAAAGCC AGGAGAAACC 
CATGAAAGCA CACAAAGGTC TAACCAAGTC ATCGAGATTT GTCTTGAGGG TCGACGGGAT 
TATAATCTAC AATCCAAAAA TGATTCCTCA AATAGCTGCA TTTCATCTGA TTCTCATCAT 
CAGTATATCC AACGTACATC ATCAGATAAG ACATACTCCC TATCAACCAC AGACATAAGA 
GATAGCCATC CATCCTATGG CCATGCTCCA TACCATGCAC AGCAGTACAG TGACGAAGGC 
ACTTTCCATG CTCAAACTAG AACAGCAATG AAACCTCCAT TCAGTGGCTA TTTCAGTAGT 
ATGCAGGAAC TGCACACAAA CAACCACATG CAACTCAACA GCCAGAATCA AACTGGAAGA 
CCAGCTGCAT CACTGTCAGT TAATTTAGGA GTCAATTCCC ACCATATCTC TCAGGGATTA 
ACACAGGCTT CTTTAATGAG AGCTGATGAC TCTAAAGCCT CTTCAGTCTC AGAAATGTCC 
CACAATGGAC GGGACAGAAT GTCCATAGGT TCTCAAGGAG GGAATAAAGA GCGGTATTTC 
CCTCCTCAAT CGCAGCACCA TGATAATAAA GAAAGAAGTG CCTCCTTTAA ACAAAATGAA 
AACCAACATT ATGTTTCTGC ACCAAGTAAT CCCTTGAATA TGTCTGATTC AACAAAACAT 
AATGAGCTTA GGGGATGCCA AAAGCAGCAC CATGTGCATG GCTCAGATGA GCTTTCTGGT 
AGTTATCCAC CCAGTAAGCA GCCCGAGCAC AGGAGAAGTG CTGGCCATCT TCATCATAAG 
GAATACTCGC AACAACCCTT TCCAACTAAA AGCGAATCAA AGATATGTCC CCAGAAAACA 
CCTATGTTGT ATTCTCTGGC CCAAGGGCAC AATGACACAG ATGACTGTTC TAATCAGATC 
AAAAGTGGAA GCGCACAACA TGAAGCCCTT GACAGTCTCA GTAGTAAACA GGTTAGACGG 
AATGACCGAT TTGCCACCAC TTTGCGCAAC GAGATCCAGA TGAGAAGGGC GCATCTCCAA 
AAGAGTCAAA GTGCAGCCAC TTTAGAAAGT CCAGTTGAAA CTGTAGAAGA GTCTGCTTTG 
TGGAAGACCA CAGGGACTGC TTCTCCCTCA TCAGATGGCT GCTTTTCCAT TTCTTATAAA 
GACCATCTTA AAGAAGCTCA GGCCAGAGTC CTCCAGGCTA CATCATTCAG GAGAAAAGAC 
CTAGAGCCAG TATTATTTGA GCATCCAGGT ACTGAAGGTC CCACACGAAA AGATGTACCT 
CCTTCAAGTG TCTCAGAGGT CACACCTAGC AAACCCACCT CAGGGAGTAA TCATGTGCTT 
CGCATTGGAG GCCGTAAGCG GATATCTGCT GAAAAGAAAG TGACGTCTTT TTCTGAGCCA 
GATAAAATTC ATGAAGTAGG AGCTGATGAA CGCTCCACTA TGCCTGAAAA TGTTGCACAC 
TTAGAAAATC AACAAAGATT CTTTGAGACA ACAGGGAAAC CAGTTTTCCC TAAGCCTATG 
CCAAAGCAAA ACCTGCAGAT ATCAGAAGAC CCCAGACTGT CCAAGCCTGG AGGCATGCAC 
TATTCTGCAA ACAGTGATAT GGCAGGGCGG AATAATGGTG AAAGCTCAAC CCCCATTGAA 
CACCACATTA ATGAAGGTCT GGATGGCCCT AACTCAGTGA ACCATCAAGC ATTGCTAGAA 
CAACAGCGAC TAGGCACATT TGCAGAGTAT GAGGCCAAAT GGAACACCCA GAGGAAGACT 
ACAGAACCAA GAGTATCTGG CCGATACCAC TCTGCTGATA ACATCCTCGA TACAGGAAAA 
GAGCGACGGA GTAACCCCAC CTGTGTGCAT GAAAGATCTA GATCCTCACC TTCTGCTGAC 
TTTTATGGGC AGAAACTTCC ACCACAAGAA AAGAAGTCAG CTGACTGTTT CAAATCTGAA 
AAGAGTCCTT GCCAACAGGA CAAGAATAGT ACAAGTCAAA ATTTCTACCC ATCTGTTTCT 
TTGGAGCACA GCCACTGGTT CTATACACCC AGCAGGTTAA ATGAAAAAGG ATATAGTGAA 
ATCTTATGCA AGGAGAAACC AGAAACACCT CCTGAAGAAC CTGAGAAAAA GACTGTAGAT 
CCTCCCTCCA GCCATCATAA GCCTGCACTA CATTTCCCTG TCCATAGCAC TCGCAGTGAA 
CCATCAGCCC TCTCCAAAAA CAAGAGCTCA TTTCTCTTGC CCCATCTGGA GAAGTACAAA 
TGCCCTGAGG GAACACCTCC TCCTCTTAGC AATATTCAGG AGGTCCAGCC TGGATCACAA 
GGAGGAGCTC TGGCCCCAAT TTCCCCAGTC AACAATCAAA GCTCCGCCCC AGTTTCAGCC 
CCCATTCCTT GGAAGAGTCC AGAGTACAGC AAAGGGCCTG TCGGGGAGGA GGAGCAACAC 
CAAGAGCTTG TGCCTCCTCC CTTTCCACCT CCTCCCCCTC CCGTTGTCCT GCCCACCCAA 
CAAACCGCTG GCCCGACCCA GCCCACAATG GATGGGCAGC GTTCGCCCTC GCCCCAGTTC 
GCTCCCCAGA GGCTGACTGA CAAGCCCCCT GTCTCCGTCT CCTCACAGGA TGAAGCTCCT 
GGAAGGATGG ATCAGATTAA AGATGAGAAC ACATCTGTGA AGAAAGTGCC CATTAAGATA 
GTCCATTCAG AGAGTGACAC GGAAAAAGAA AGTCGGCAAT ATCTAGACCT GCCCAGCGAG 
ACGCCTGTCA ACTCGCAGGG ACTTGTGGGA GCTCATCTTC AAAGTTTGGG GAACCCGGAT 
CAGTCATACT CTTTGTTTTG TACATACACC AGACAAAAAG ATCAGGTTCC TGGTCTTAGA 
GACCCAGACA TGGGCCCTCT AAAAGACCAG GGGCCACAAA GCAATATAAA CCCAGTGTCT 
TATGTGGAAC CTGGCCTTCA GACAGGTCCA CCATTGGATC AGAGTACCAA TGGAGTGTCT 
TTGCATCCTT CACATTCAGA GGATGATAAA AGAAAGGAGC TGGCTAGAGA CATAATGGAC 
AAAGACAAGT CCCTCGCTGA CATTTTAGAC CAGAGTAAGA TGAAGACCAC TATGGATCTG 
ATGGAGGGGA TTTTCCCTCA AGGAGATCAG CTACTGGAGG AGGCCCAACA GCGTAGGAAG 
GCTCAACCTA AACCACTCTC CCCTCGAAAC TCTGTTGAGA AGAAAGAGGA AGACAGCCTG 
GTGGCGGCCG CTGCTTTAGT GAGCAACTCA ACCTACTACA GCACATCTGC ACCCAAAGCA 
GAGCTGCTGA TCAAGATGAA GGACATGCAG GACCAGAGCG TTGAACACAA CTCAGAAGAG 
GAGCTGGAGG AGGACAATGA AAGTGACCTC GCCTGCAAGA AGCACGAGTT GATTGAAAGT 
CTCAGTAAGA AGTTGCAGGT CTTAAAAGAA GCTCAGGAGA GCCTGCAGGA GGACGTGCAG 
GACAACAACG CTCTTGGAGA AGAAGTGGAG GCCATCGTAC AGGGCGTCTG CAAGCCTAAT 
GAACTCGACA AATTCCGCAT GTTCGTCGGG GATCTTGATA AAGTGGTCAA CCTTCTGCTG 
TCTCTGTCAG GACGTTTGGC CAGGGTAGAG AATGCCCTGA ACAGCCTGGA GGAAGATGCT 
TCGCTGGAGG AAAGGCGCAC TCTGACAGAG AAACGCAAAC TGCTGATTCG TCAGCACGAG 
GATGCTAAGG AGCTGAAGGA GAACCTGGAC AGGAGAGAGC GTGTGGTCTA CGACATTCTG 
GCCAGTTACC TGCCTGAGGA GAGTATGGCT GACTACGAGC ACTTTGTCAA GATGAAATCC 
GCTCTTATTA TCGAGCAACG CAAGCTGGAG GACAAGATCA AACTGGGAGA TGAGCAGCTC 
AAGTGTCTGA TGGACAGTCT CCCAATGGAC CAGAGAATAA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212126.1
CDS	859..5646
Translation	MKMVDIVAQKMPSESDVHMARSFLTKILRSSMRKNRFKGRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356637.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356637.1

ORF Insert Sequence:

ATGAAAATGG TGGATATTGT AGCGCAGAAA ATGCCATCAG AGAGCGACGT GCATATGGCC 
CGGAGCTTTC TCACGAAGAT TTTACGAAGT TCCATGAGAA AGAATCGGTT TAAAGGGAGA 
AATGAACCCA TGAGCAGACC TCACTCCTGG CATGCCACCA AGTTCAACGA GACCCATTCA 
GAAGCCAAAA CCCAGTCCAC ACCCTCACCA GTTTGGCAAA CAAGATATGA TGCAAGTTCA 
TTCTCTTCTG AACTCTCAAC TGGCTGGGAT CAAACAAATC TACGGAGAGT ATCCGATCAG 
TTCAGTTCTT TGGGGAGTAT GGACAGCCTA GAACATAGTT CTCATCCCTA TCCACCTGGC 
CGTCTTTCCC CAAACAAATC CAACAATAAT AGCATTGAAC ATTTGGGTAG TGGTAAACGG 
GACTCGGCAT ACAGCTCTTT TTCTACAAGT TCGGGAACCC CAGATTACAC ACTGTCCAAA 
AGTAACACGG CATCCACTGA GAACATGCTG TACAAAATAA ATCAGTGGGA TTCAAGTGCC 
AGGCACAGCA ATGGCAGGCA CAGCCAAAAC CTAAGCGAAG GGGTACGACA AGATGAGAGG 
CCTGGATACC TGCACCACCT CTCAAGTACA GGTAACCACG AGATCCCAAG AGCTGAAGAA 
CTGCCTGGCA ACCGTCATTC AAGCTCTGGA AGAGTTAATA TTGGACCTGT CTGGCATGTT 
CCAGATATGA AGAAAAACAT GATATCATCT ACTCCACCTC CCACTCCACC CACACGCAGT 
GATAGCTTTG CAGCTACCAA GGTTCACGAG AAGGGTCTGA TCACAGGTAC TTCAGAGGGC 
CTTGGTGGTC ATTCTCAAGT AAAGCCTCAA GCGAAACCAC AACAAAAGCC AGGAGAAACC 
CATGAAAGCA CACAAAGGTC TAACCAAGTC ATCGAGATTT GTCTTGAGGG TCGACGGGAT 
TATAATCTAC AATCCAAAAA TGATTCCTCA AATAGCTGCA TTTCATCTGA TTCTCATCAT 
CAGTATATCC AACGTACATC ATCAGATAAG ACATACTCCC TATCAACCAC AGACATAAGA 
GATAGCCATC CATCCTATGG CCATGCTCCA TACCATGCAC AGCAGTACAG TGACGAAGGC 
ACTTTCCATG CTCAAACTAG AACAGCAATG AAACCTCCAT TCAGTGGCTA TTTCAGTAGT 
ATGCAGGAAC TGCACACAAA CAACCACATG CAACTCAACA GCCAGAATCA AACTGGAAGA 
CCAGCTGCAT CACTGTCAGT TAATTTAGGA GTCAATTCCC ACCATATCTC TCAGGGATTA 
ACACAGGCTT CTTTAATGAG AGCTGATGAC TCTAAAGCCT CTTCAGTCTC AGAAATGTCC 
CACAATGGAC GGGACAGAAT GTCCATAGGT TCTCAAGGAG GGAATAAAGA GCGGTATTTC 
CCTCCTCAAT CGCAGCACCA TGATAATAAA GAAAGAAGTG CCTCCTTTAA ACAAAATGAA 
AACCAACATT ATGTTTCTGC ACCAAGTAAT CCCTTGAATA TGTCTGATTC AACAAAACAT 
AATGAGCTTA GGGGATGCCA AAAGCAGCAC CATGTGCATG GCTCAGATGA GCTTTCTGGT 
AGTTATCCAC CCAGTAAGCA GCCCGAGCAC AGGAGAAGTG CTGGCCATCT TCATCATAAG 
GAATACTCGC AACAACCCTT TCCAACTAAA AGCGAATCAA AGATATGTCC CCAGAAAACA 
CCTATGTTGT ATTCTCTGGC CCAAGGGCAC AATGACACAG ATGACTGTTC TAATCAGATC 
AAAAGTGGAA GCGCACAACA TGAAGCCCTT GACAGTCTCA GTAGTAAACA GGTTAGACGG 
AATGACCGAT TTGCCACCAC TTTGCGCAAC GAGATCCAGA TGAGAAGGGC GCATCTCCAA 
AAGAGTCAAA GTGCAGCCAC TTTAGAAAGT CCAGTTGAAA CTGTAGAAGA GTCTGCTTTG 
TGGAAGACCA CAGGGACTGC TTCTCCCTCA TCAGATGGCT GCTTTTCCAT TTCTTATAAA 
GACCATCTTA AAGAAGCTCA GGCCAGAGTC CTCCAGGCTA CATCATTCAG GAGAAAAGAC 
CTAGAGCCAG TATTATTTGA GCATCCAGGT ACTGAAGGTC CCACACGAAA AGATGTACCT 
CCTTCAAGTG TCTCAGAGGT CACACCTAGC AAACCCACCT CAGGGAGTAA TCATGTGCTT 
CGCATTGGAG GCCGTAAGCG GATATCTGCT GAAAAGAAAG TGACGTCTTT TTCTGAGCCA 
GATAAAATTC ATGAAGTAGG AGCTGATGAA CGCTCCACTA TGCCTGAAAA TGTTGCACAC 
TTAGAAAATC AACAAAGATT CTTTGAGACA ACAGGGAAAC CAGTTTTCCC TAAGCCTATG 
CCAAAGCAAA ACCTGCAGAT ATCAGAAGAC CCCAGACTGT CCAAGCCTGG AGGCATGCAC 
TATTCTGCAA ACAGTGATAT GGCAGGGCGG AATAATGGTG AAAGCTCAAC CCCCATTGAA 
CACCACATTA ATGAAGGTCT GGATGGCCCT AACTCAGTGA ACCATCAAGC ATTGCTAGAA 
CAACAGCGAC TAGGCACATT TGCAGAGTAT GAGGCCAAAT GGAACACCCA GAGGAAGACT 
ACAGAACCAA GAGTATCTGG CCGATACCAC TCTGCTGATA ACATCCTCGA TACAGGAAAA 
GAGCGACGGA GTAACCCCAC CTGTGTGCAT GAAAGATCTA GATCCTCACC TTCTGCTGAC 
TTTTATGGGC AGAAACTTCC ACCACAAGAA AAGAAGTCAG CTGACTGTTT CAAATCTGAA 
AAGAGTCCTT GCCAACAGGA CAAGAATAGT ACAAGTCAAA ATTTCTACCC ATCTGTTTCT 
TTGGAGCACA GCCACTGGTT CTATACACCC AGCAGGTTAA ATGAAAAAGG ATATAGTGAA 
ATCTTATGCA AGGAGAAACC AGAAACACCT CCTGAAGAAC CTGAGAAAAA GACTGTAGAT 
CCTCCCTCCA GCCATCATAA GCCTGCACTA CATTTCCCTG TCCATAGCAC TCGCAGTGAA 
CCATCAGCCC TCTCCAAAAA CAAGAGCTCA TTTCTCTTGC CCCATCTGGA GAAGTACAAA 
TGCCCTGAGG GAACACCTCC TCCTCTTAGC AATATTCAGG AGGTCCAGCC TGGATCACAA 
GGAGGAGCTC TGGCCCCAAT TTCCCCAGTC AACAATCAAA GCTCCGCCCC AGTTTCAGCC 
CCCATTCCTT GGAAGAGTCC AGAGTACAGC AAAGGGCCTG TCGGGGAGGA GGAGCAACAC 
CAAGAGCTTG TGCCTCCTCC CTTTCCACCT CCTCCCCCTC CCGTTGTCCT GCCCACCCAA 
CAAACCGCTG GCCCGACCCA GCCCACAATG GATGGGCAGC GTTCGCCCTC GCCCCAGTTC 
GCTCCCCAGA GGCTGACTGA CAAGCCCCCT GTCTCCGTCT CCTCACAGGA TGAAGCTCCT 
GGAAGGATGG ATCAGATTAA AGATGAGAAC ACATCTGTGA AGAAAGTGCC CATTAAGATA 
GTCCATTCAG AGAGTGACAC GGAAAAAGAA AGTCGGCAAT ATCTAGACCT GCCCAGCGAG 
ACGCCTGTCA ACTCGCAGGG ACTTGTGGGA GCTCATCTTC AAAGTTTGGG GAACCCGGAT 
CAGTCATACT CTTTGTTTTG TACATACACC AGACAAAAAG ATCAGGTTCC TGGTCTTAGA 
GACCCAGACA TGGGCCCTCT AAAAGACCAG GGGCCACAAA GCAATATAAA CCCAGTGTCT 
TATGTGGAAC CTGGCCTTCA GACAGGTCCA CCATTGGATC AGAGTACCAA TGGAGTGTCT 
TTGCATCCTT CACATTCAGA GGATGATAAA AGAAAGGAGC TGGCTAGAGA CATAATGGAC 
AAAGACAAGT CCCTCGCTGA CATTTTAGAC CAGAGTAAGA TGAAGACCAC TATGGATCTG 
ATGGAGGGGA TTTTCCCTCA AGGAGATCAG CTACTGGAGG AGGCCCAACA GCGTAGGAAG 
GCTCAACCTA AACCACTCTC CCCTCGAAAC TCTGTTGAGA AGAAAGAGGA AGACAGCCTG 
GTGGCGGCCG CTGCTTTAGT GAGCAACTCA ACCTACTACA GCACATCTGC ACCCAAAGCA 
GAGCTGCTGA TCAAGATGAA GGACATGCAG GACCAGAGCG TTGAACACAA CTCAGAAGAG 
GAGCTGGAGG AGGACAATGA AAGTGACCTC GCCTGCAAGA AGCACGAGTT GATTGAAAGT 
CTCAGTAAGA AGTTGCAGGT CTTAAAAGAA GCTCAGGAGA GCCTGCAGGA GGACGTGCAG 
GACAACAACG CTCTTGGAGA AGAAGTGGAG GCCATCGTAC AGGGCGTCTG CAAGCCTAAT 
GAACTCGACA AATTCCGCAT GTTCGTCGGG GATCTTGATA AAGTGGTCAA CCTTCTGCTG 
TCTCTGTCAG GACGTTTGGC CAGGGTAGAG AATGCCCTGA ACAGCCTGGA GGAAGATGCT 
TCGCTGGAGG AAAGGCGCAC TCTGACAGAG AAACGCAAAC TGCTGATTCG TCAGCACGAG 
GATGCTAAGG AGCTGAAGGA GAACCTGGAC AGGAGAGAGC GTGTGGTCTA CGACATTCTG 
GCCAGTTACC TGCCTGAGGA GAGTATGGCT GACTACGAGC ACTTTGTCAA GATGAAATCC 
GCTCTTATTA TCGAGCAACG CAAGCTGGAG GACAAGATCA AACTGGGAGA TGAGCAGCTC 
AAGTGTCTGA TGGACAGTCT CCCAATGGAC CAGAGAATAA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356638.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212127.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X7, mRNA.

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CloneID	ODa59476
Clone ID Related Accession (Same CDS sequence)	XM_017356637.2 , XM_017356637.1 , XM_017356638.1 , XM_017356638.2 , XM_021477154.1
Accession Version	XM_017356638.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom2 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGG TGGATATTGT AGCGCAGAAA ATGCCATCAG AGAGCGACGT GCATATGGCC 
CGGAGCTTTC TCACGAAGAT TTTACGAAGT TCCATGAGAA AGAATCGGTT TAAAGGGAGA 
AATGAACCCA TGAGCAGACC TCACTCCTGG CATGCCACCA AGTTCAACGA GACCCATTCA 
GAAGCCAAAA CCCAGTCCAC ACCCTCACCA GTTTGGCAAA CAAGATATGA TGCAAGTTCA 
TTCTCTTCTG AACTCTCAAC TGGCTGGGAT CAAACAAATC TACGGAGAGT ATCCGATCAG 
TTCAGTTCTT TGGGGAGTAT GGACAGCCTA GAACATAGTT CTCATCCCTA TCCACCTGGC 
CGTCTTTCCC CAAACAAATC CAACAATAAT AGCATTGAAC ATTTGGGTAG TGGTAAACGG 
GACTCGGCAT ACAGCTCTTT TTCTACAAGT TCGGGAACCC CAGATTACAC ACTGTCCAAA 
AGTAACACGG CATCCACTGA GAACATGCTG TACAAAATAA ATCAGTGGGA TTCAAGTGCC 
AGGCACAGCA ATGGCAGGCA CAGCCAAAAC CTAAGCGAAG GGGTACGACA AGATGAGAGG 
CCTGGATACC TGCACCACCT CTCAAGTACA GGTAACCACG AGATCCCAAG AGCTGAAGAA 
CTGCCTGGCA ACCGTCATTC AAGCTCTGGA AGAGTTAATA TTGGACCTGT CTGGCATGTT 
CCAGATATGA AGAAAAACAT GATATCATCT ACTCCACCTC CCACTCCACC CACACGCAGT 
GATAGCTTTG CAGCTACCAA GGTTCACGAG AAGGGTCTGA TCACAGGTAC TTCAGAGGGC 
CTTGGTGGTC ATTCTCAAGT AAAGCCTCAA GCGAAACCAC AACAAAAGCC AGGAGAAACC 
CATGAAAGCA CACAAAGGTC TAACCAAGTC ATCGAGATTT GTCTTGAGGG TCGACGGGAT 
TATAATCTAC AATCCAAAAA TGATTCCTCA AATAGCTGCA TTTCATCTGA TTCTCATCAT 
CAGTATATCC AACGTACATC ATCAGATAAG ACATACTCCC TATCAACCAC AGACATAAGA 
GATAGCCATC CATCCTATGG CCATGCTCCA TACCATGCAC AGCAGTACAG TGACGAAGGC 
ACTTTCCATG CTCAAACTAG AACAGCAATG AAACCTCCAT TCAGTGGCTA TTTCAGTAGT 
ATGCAGGAAC TGCACACAAA CAACCACATG CAACTCAACA GCCAGAATCA AACTGGAAGA 
CCAGCTGCAT CACTGTCAGT TAATTTAGGA GTCAATTCCC ACCATATCTC TCAGGGATTA 
ACACAGGCTT CTTTAATGAG AGCTGATGAC TCTAAAGCCT CTTCAGTCTC AGAAATGTCC 
CACAATGGAC GGGACAGAAT GTCCATAGGT TCTCAAGGAG GGAATAAAGA GCGGTATTTC 
CCTCCTCAAT CGCAGCACCA TGATAATAAA GAAAGAAGTG CCTCCTTTAA ACAAAATGAA 
AACCAACATT ATGTTTCTGC ACCAAGTAAT CCCTTGAATA TGTCTGATTC AACAAAACAT 
AATGAGCTTA GGGGATGCCA AAAGCAGCAC CATGTGCATG GCTCAGATGA GCTTTCTGGT 
AGTTATCCAC CCAGTAAGCA GCCCGAGCAC AGGAGAAGTG CTGGCCATCT TCATCATAAG 
GAATACTCGC AACAACCCTT TCCAACTAAA AGCGAATCAA AGATATGTCC CCAGAAAACA 
CCTATGTTGT ATTCTCTGGC CCAAGGGCAC AATGACACAG ATGACTGTTC TAATCAGATC 
AAAAGTGGAA GCGCACAACA TGAAGCCCTT GACAGTCTCA GTAGTAAACA GGTTAGACGG 
AATGACCGAT TTGCCACCAC TTTGCGCAAC GAGATCCAGA TGAGAAGGGC GCATCTCCAA 
AAGAGTCAAA GTGCAGCCAC TTTAGAAAGT CCAGTTGAAA CTGTAGAAGA GTCTGCTTTG 
TGGAAGACCA CAGGGACTGC TTCTCCCTCA TCAGATGGCT GCTTTTCCAT TTCTTATAAA 
GACCATCTTA AAGAAGCTCA GGCCAGAGTC CTCCAGGCTA CATCATTCAG GAGAAAAGAC 
CTAGAGCCAG TATTATTTGA GCATCCAGGT ACTGAAGGTC CCACACGAAA AGATGTACCT 
CCTTCAAGTG TCTCAGAGGT CACACCTAGC AAACCCACCT CAGGGAGTAA TCATGTGCTT 
CGCATTGGAG GCCGTAAGCG GATATCTGCT GAAAAGAAAG TGACGTCTTT TTCTGAGCCA 
GATAAAATTC ATGAAGTAGG AGCTGATGAA CGCTCCACTA TGCCTGAAAA TGTTGCACAC 
TTAGAAAATC AACAAAGATT CTTTGAGACA ACAGGGAAAC CAGTTTTCCC TAAGCCTATG 
CCAAAGCAAA ACCTGCAGAT ATCAGAAGAC CCCAGACTGT CCAAGCCTGG AGGCATGCAC 
TATTCTGCAA ACAGTGATAT GGCAGGGCGG AATAATGGTG AAAGCTCAAC CCCCATTGAA 
CACCACATTA ATGAAGGTCT GGATGGCCCT AACTCAGTGA ACCATCAAGC ATTGCTAGAA 
CAACAGCGAC TAGGCACATT TGCAGAGTAT GAGGCCAAAT GGAACACCCA GAGGAAGACT 
ACAGAACCAA GAGTATCTGG CCGATACCAC TCTGCTGATA ACATCCTCGA TACAGGAAAA 
GAGCGACGGA GTAACCCCAC CTGTGTGCAT GAAAGATCTA GATCCTCACC TTCTGCTGAC 
TTTTATGGGC AGAAACTTCC ACCACAAGAA AAGAAGTCAG CTGACTGTTT CAAATCTGAA 
AAGAGTCCTT GCCAACAGGA CAAGAATAGT ACAAGTCAAA ATTTCTACCC ATCTGTTTCT 
TTGGAGCACA GCCACTGGTT CTATACACCC AGCAGGTTAA ATGAAAAAGG ATATAGTGAA 
ATCTTATGCA AGGAGAAACC AGAAACACCT CCTGAAGAAC CTGAGAAAAA GACTGTAGAT 
CCTCCCTCCA GCCATCATAA GCCTGCACTA CATTTCCCTG TCCATAGCAC TCGCAGTGAA 
CCATCAGCCC TCTCCAAAAA CAAGAGCTCA TTTCTCTTGC CCCATCTGGA GAAGTACAAA 
TGCCCTGAGG GAACACCTCC TCCTCTTAGC AATATTCAGG AGGTCCAGCC TGGATCACAA 
GGAGGAGCTC TGGCCCCAAT TTCCCCAGTC AACAATCAAA GCTCCGCCCC AGTTTCAGCC 
CCCATTCCTT GGAAGAGTCC AGAGTACAGC AAAGGGCCTG TCGGGGAGGA GGAGCAACAC 
CAAGAGCTTG TGCCTCCTCC CTTTCCACCT CCTCCCCCTC CCGTTGTCCT GCCCACCCAA 
CAAACCGCTG GCCCGACCCA GCCCACAATG GATGGGCAGC GTTCGCCCTC GCCCCAGTTC 
GCTCCCCAGA GGCTGACTGA CAAGCCCCCT GTCTCCGTCT CCTCACAGGA TGAAGCTCCT 
GGAAGGATGG ATCAGATTAA AGATGAGAAC ACATCTGTGA AGAAAGTGCC CATTAAGATA 
GTCCATTCAG AGAGTGACAC GGAAAAAGAA AGTCGGCAAT ATCTAGACCT GCCCAGCGAG 
ACGCCTGTCA ACTCGCAGGG ACTTGTGGGA GCTCATCTTC AAAGTTTGGG GAACCCGGAT 
CAGTCATACT CTTTGTTTTG TACATACACC AGACAAAAAG ATCAGGTTCC TGGTCTTAGA 
GACCCAGACA TGGGCCCTCT AAAAGACCAG GGGCCACAAA GCAATATAAA CCCAGTGTCT 
TATGTGGAAC CTGGCCTTCA GACAGGTCCA CCATTGGATC AGAGTACCAA TGGAGTGTCT 
TTGCATCCTT CACATTCAGA GGATGATAAA AGAAAGGAGC TGGCTAGAGA CATAATGGAC 
AAAGACAAGT CCCTCGCTGA CATTTTAGAC CAGAGTAAGA TGAAGACCAC TATGGATCTG 
ATGGAGGGGA TTTTCCCTCA AGGAGATCAG CTACTGGAGG AGGCCCAACA GCGTAGGAAG 
GCTCAACCTA AACCACTCTC CCCTCGAAAC TCTGTTGAGA AGAAAGAGGA AGACAGCCTG 
GTGGCGGCCG CTGCTTTAGT GAGCAACTCA ACCTACTACA GCACATCTGC ACCCAAAGCA 
GAGCTGCTGA TCAAGATGAA GGACATGCAG GACCAGAGCG TTGAACACAA CTCAGAAGAG 
GAGCTGGAGG AGGACAATGA AAGTGACCTC GCCTGCAAGA AGCACGAGTT GATTGAAAGT 
CTCAGTAAGA AGTTGCAGGT CTTAAAAGAA GCTCAGGAGA GCCTGCAGGA GGACGTGCAG 
GACAACAACG CTCTTGGAGA AGAAGTGGAG GCCATCGTAC AGGGCGTCTG CAAGCCTAAT 
GAACTCGACA AATTCCGCAT GTTCGTCGGG GATCTTGATA AAGTGGTCAA CCTTCTGCTG 
TCTCTGTCAG GACGTTTGGC CAGGGTAGAG AATGCCCTGA ACAGCCTGGA GGAAGATGCT 
TCGCTGGAGG AAAGGCGCAC TCTGACAGAG AAACGCAAAC TGCTGATTCG TCAGCACGAG 
GATGCTAAGG AGCTGAAGGA GAACCTGGAC AGGAGAGAGC GTGTGGTCTA CGACATTCTG 
GCCAGTTACC TGCCTGAGGA GAGTATGGCT GACTACGAGC ACTTTGTCAA GATGAAATCC 
GCTCTTATTA TCGAGCAACG CAAGCTGGAG GACAAGATCA AACTGGGAGA TGAGCAGCTC 
AAGTGTCTGA TGGACAGTCT CCCAATGGAC CAGAGAATAA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212127.1
CDS	159..4946
Translation	MKMVDIVAQKMPSESDVHMARSFLTKILRSSMRKNRFKGRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356638.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356638.1

ORF Insert Sequence:

ATGAAAATGG TGGATATTGT AGCGCAGAAA ATGCCATCAG AGAGCGACGT GCATATGGCC 
CGGAGCTTTC TCACGAAGAT TTTACGAAGT TCCATGAGAA AGAATCGGTT TAAAGGGAGA 
AATGAACCCA TGAGCAGACC TCACTCCTGG CATGCCACCA AGTTCAACGA GACCCATTCA 
GAAGCCAAAA CCCAGTCCAC ACCCTCACCA GTTTGGCAAA CAAGATATGA TGCAAGTTCA 
TTCTCTTCTG AACTCTCAAC TGGCTGGGAT CAAACAAATC TACGGAGAGT ATCCGATCAG 
TTCAGTTCTT TGGGGAGTAT GGACAGCCTA GAACATAGTT CTCATCCCTA TCCACCTGGC 
CGTCTTTCCC CAAACAAATC CAACAATAAT AGCATTGAAC ATTTGGGTAG TGGTAAACGG 
GACTCGGCAT ACAGCTCTTT TTCTACAAGT TCGGGAACCC CAGATTACAC ACTGTCCAAA 
AGTAACACGG CATCCACTGA GAACATGCTG TACAAAATAA ATCAGTGGGA TTCAAGTGCC 
AGGCACAGCA ATGGCAGGCA CAGCCAAAAC CTAAGCGAAG GGGTACGACA AGATGAGAGG 
CCTGGATACC TGCACCACCT CTCAAGTACA GGTAACCACG AGATCCCAAG AGCTGAAGAA 
CTGCCTGGCA ACCGTCATTC AAGCTCTGGA AGAGTTAATA TTGGACCTGT CTGGCATGTT 
CCAGATATGA AGAAAAACAT GATATCATCT ACTCCACCTC CCACTCCACC CACACGCAGT 
GATAGCTTTG CAGCTACCAA GGTTCACGAG AAGGGTCTGA TCACAGGTAC TTCAGAGGGC 
CTTGGTGGTC ATTCTCAAGT AAAGCCTCAA GCGAAACCAC AACAAAAGCC AGGAGAAACC 
CATGAAAGCA CACAAAGGTC TAACCAAGTC ATCGAGATTT GTCTTGAGGG TCGACGGGAT 
TATAATCTAC AATCCAAAAA TGATTCCTCA AATAGCTGCA TTTCATCTGA TTCTCATCAT 
CAGTATATCC AACGTACATC ATCAGATAAG ACATACTCCC TATCAACCAC AGACATAAGA 
GATAGCCATC CATCCTATGG CCATGCTCCA TACCATGCAC AGCAGTACAG TGACGAAGGC 
ACTTTCCATG CTCAAACTAG AACAGCAATG AAACCTCCAT TCAGTGGCTA TTTCAGTAGT 
ATGCAGGAAC TGCACACAAA CAACCACATG CAACTCAACA GCCAGAATCA AACTGGAAGA 
CCAGCTGCAT CACTGTCAGT TAATTTAGGA GTCAATTCCC ACCATATCTC TCAGGGATTA 
ACACAGGCTT CTTTAATGAG AGCTGATGAC TCTAAAGCCT CTTCAGTCTC AGAAATGTCC 
CACAATGGAC GGGACAGAAT GTCCATAGGT TCTCAAGGAG GGAATAAAGA GCGGTATTTC 
CCTCCTCAAT CGCAGCACCA TGATAATAAA GAAAGAAGTG CCTCCTTTAA ACAAAATGAA 
AACCAACATT ATGTTTCTGC ACCAAGTAAT CCCTTGAATA TGTCTGATTC AACAAAACAT 
AATGAGCTTA GGGGATGCCA AAAGCAGCAC CATGTGCATG GCTCAGATGA GCTTTCTGGT 
AGTTATCCAC CCAGTAAGCA GCCCGAGCAC AGGAGAAGTG CTGGCCATCT TCATCATAAG 
GAATACTCGC AACAACCCTT TCCAACTAAA AGCGAATCAA AGATATGTCC CCAGAAAACA 
CCTATGTTGT ATTCTCTGGC CCAAGGGCAC AATGACACAG ATGACTGTTC TAATCAGATC 
AAAAGTGGAA GCGCACAACA TGAAGCCCTT GACAGTCTCA GTAGTAAACA GGTTAGACGG 
AATGACCGAT TTGCCACCAC TTTGCGCAAC GAGATCCAGA TGAGAAGGGC GCATCTCCAA 
AAGAGTCAAA GTGCAGCCAC TTTAGAAAGT CCAGTTGAAA CTGTAGAAGA GTCTGCTTTG 
TGGAAGACCA CAGGGACTGC TTCTCCCTCA TCAGATGGCT GCTTTTCCAT TTCTTATAAA 
GACCATCTTA AAGAAGCTCA GGCCAGAGTC CTCCAGGCTA CATCATTCAG GAGAAAAGAC 
CTAGAGCCAG TATTATTTGA GCATCCAGGT ACTGAAGGTC CCACACGAAA AGATGTACCT 
CCTTCAAGTG TCTCAGAGGT CACACCTAGC AAACCCACCT CAGGGAGTAA TCATGTGCTT 
CGCATTGGAG GCCGTAAGCG GATATCTGCT GAAAAGAAAG TGACGTCTTT TTCTGAGCCA 
GATAAAATTC ATGAAGTAGG AGCTGATGAA CGCTCCACTA TGCCTGAAAA TGTTGCACAC 
TTAGAAAATC AACAAAGATT CTTTGAGACA ACAGGGAAAC CAGTTTTCCC TAAGCCTATG 
CCAAAGCAAA ACCTGCAGAT ATCAGAAGAC CCCAGACTGT CCAAGCCTGG AGGCATGCAC 
TATTCTGCAA ACAGTGATAT GGCAGGGCGG AATAATGGTG AAAGCTCAAC CCCCATTGAA 
CACCACATTA ATGAAGGTCT GGATGGCCCT AACTCAGTGA ACCATCAAGC ATTGCTAGAA 
CAACAGCGAC TAGGCACATT TGCAGAGTAT GAGGCCAAAT GGAACACCCA GAGGAAGACT 
ACAGAACCAA GAGTATCTGG CCGATACCAC TCTGCTGATA ACATCCTCGA TACAGGAAAA 
GAGCGACGGA GTAACCCCAC CTGTGTGCAT GAAAGATCTA GATCCTCACC TTCTGCTGAC 
TTTTATGGGC AGAAACTTCC ACCACAAGAA AAGAAGTCAG CTGACTGTTT CAAATCTGAA 
AAGAGTCCTT GCCAACAGGA CAAGAATAGT ACAAGTCAAA ATTTCTACCC ATCTGTTTCT 
TTGGAGCACA GCCACTGGTT CTATACACCC AGCAGGTTAA ATGAAAAAGG ATATAGTGAA 
ATCTTATGCA AGGAGAAACC AGAAACACCT CCTGAAGAAC CTGAGAAAAA GACTGTAGAT 
CCTCCCTCCA GCCATCATAA GCCTGCACTA CATTTCCCTG TCCATAGCAC TCGCAGTGAA 
CCATCAGCCC TCTCCAAAAA CAAGAGCTCA TTTCTCTTGC CCCATCTGGA GAAGTACAAA 
TGCCCTGAGG GAACACCTCC TCCTCTTAGC AATATTCAGG AGGTCCAGCC TGGATCACAA 
GGAGGAGCTC TGGCCCCAAT TTCCCCAGTC AACAATCAAA GCTCCGCCCC AGTTTCAGCC 
CCCATTCCTT GGAAGAGTCC AGAGTACAGC AAAGGGCCTG TCGGGGAGGA GGAGCAACAC 
CAAGAGCTTG TGCCTCCTCC CTTTCCACCT CCTCCCCCTC CCGTTGTCCT GCCCACCCAA 
CAAACCGCTG GCCCGACCCA GCCCACAATG GATGGGCAGC GTTCGCCCTC GCCCCAGTTC 
GCTCCCCAGA GGCTGACTGA CAAGCCCCCT GTCTCCGTCT CCTCACAGGA TGAAGCTCCT 
GGAAGGATGG ATCAGATTAA AGATGAGAAC ACATCTGTGA AGAAAGTGCC CATTAAGATA 
GTCCATTCAG AGAGTGACAC GGAAAAAGAA AGTCGGCAAT ATCTAGACCT GCCCAGCGAG 
ACGCCTGTCA ACTCGCAGGG ACTTGTGGGA GCTCATCTTC AAAGTTTGGG GAACCCGGAT 
CAGTCATACT CTTTGTTTTG TACATACACC AGACAAAAAG ATCAGGTTCC TGGTCTTAGA 
GACCCAGACA TGGGCCCTCT AAAAGACCAG GGGCCACAAA GCAATATAAA CCCAGTGTCT 
TATGTGGAAC CTGGCCTTCA GACAGGTCCA CCATTGGATC AGAGTACCAA TGGAGTGTCT 
TTGCATCCTT CACATTCAGA GGATGATAAA AGAAAGGAGC TGGCTAGAGA CATAATGGAC 
AAAGACAAGT CCCTCGCTGA CATTTTAGAC CAGAGTAAGA TGAAGACCAC TATGGATCTG 
ATGGAGGGGA TTTTCCCTCA AGGAGATCAG CTACTGGAGG AGGCCCAACA GCGTAGGAAG 
GCTCAACCTA AACCACTCTC CCCTCGAAAC TCTGTTGAGA AGAAAGAGGA AGACAGCCTG 
GTGGCGGCCG CTGCTTTAGT GAGCAACTCA ACCTACTACA GCACATCTGC ACCCAAAGCA 
GAGCTGCTGA TCAAGATGAA GGACATGCAG GACCAGAGCG TTGAACACAA CTCAGAAGAG 
GAGCTGGAGG AGGACAATGA AAGTGACCTC GCCTGCAAGA AGCACGAGTT GATTGAAAGT 
CTCAGTAAGA AGTTGCAGGT CTTAAAAGAA GCTCAGGAGA GCCTGCAGGA GGACGTGCAG 
GACAACAACG CTCTTGGAGA AGAAGTGGAG GCCATCGTAC AGGGCGTCTG CAAGCCTAAT 
GAACTCGACA AATTCCGCAT GTTCGTCGGG GATCTTGATA AAGTGGTCAA CCTTCTGCTG 
TCTCTGTCAG GACGTTTGGC CAGGGTAGAG AATGCCCTGA ACAGCCTGGA GGAAGATGCT 
TCGCTGGAGG AAAGGCGCAC TCTGACAGAG AAACGCAAAC TGCTGATTCG TCAGCACGAG 
GATGCTAAGG AGCTGAAGGA GAACCTGGAC AGGAGAGAGC GTGTGGTCTA CGACATTCTG 
GCCAGTTACC TGCCTGAGGA GAGTATGGCT GACTACGAGC ACTTTGTCAA GATGAAATCC 
GCTCTTATTA TCGAGCAACG CAAGCTGGAG GACAAGATCA AACTGGGAGA TGAGCAGCTC 
AAGTGTCTGA TGGACAGTCT CCCAATGGAC CAGAGAATAA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356638.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212127.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa59476
Clone ID Related Accession (Same CDS sequence)	XM_017356637.2 , XM_017356637.1 , XM_017356638.1 , XM_017356638.2 , XM_021477154.1
Accession Version	XM_017356638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGG TGGATATTGT AGCGCAGAAA ATGCCATCAG AGAGCGACGT GCATATGGCC 
CGGAGCTTTC TCACGAAGAT TTTACGAAGT TCCATGAGAA AGAATCGGTT TAAAGGGAGA 
AATGAACCCA TGAGCAGACC TCACTCCTGG CATGCCACCA AGTTCAACGA GACCCATTCA 
GAAGCCAAAA CCCAGTCCAC ACCCTCACCA GTTTGGCAAA CAAGATATGA TGCAAGTTCA 
TTCTCTTCTG AACTCTCAAC TGGCTGGGAT CAAACAAATC TACGGAGAGT ATCCGATCAG 
TTCAGTTCTT TGGGGAGTAT GGACAGCCTA GAACATAGTT CTCATCCCTA TCCACCTGGC 
CGTCTTTCCC CAAACAAATC CAACAATAAT AGCATTGAAC ATTTGGGTAG TGGTAAACGG 
GACTCGGCAT ACAGCTCTTT TTCTACAAGT TCGGGAACCC CAGATTACAC ACTGTCCAAA 
AGTAACACGG CATCCACTGA GAACATGCTG TACAAAATAA ATCAGTGGGA TTCAAGTGCC 
AGGCACAGCA ATGGCAGGCA CAGCCAAAAC CTAAGCGAAG GGGTACGACA AGATGAGAGG 
CCTGGATACC TGCACCACCT CTCAAGTACA GGTAACCACG AGATCCCAAG AGCTGAAGAA 
CTGCCTGGCA ACCGTCATTC AAGCTCTGGA AGAGTTAATA TTGGACCTGT CTGGCATGTT 
CCAGATATGA AGAAAAACAT GATATCATCT ACTCCACCTC CCACTCCACC CACACGCAGT 
GATAGCTTTG CAGCTACCAA GGTTCACGAG AAGGGTCTGA TCACAGGTAC TTCAGAGGGC 
CTTGGTGGTC ATTCTCAAGT AAAGCCTCAA GCGAAACCAC AACAAAAGCC AGGAGAAACC 
CATGAAAGCA CACAAAGGTC TAACCAAGTC ATCGAGATTT GTCTTGAGGG TCGACGGGAT 
TATAATCTAC AATCCAAAAA TGATTCCTCA AATAGCTGCA TTTCATCTGA TTCTCATCAT 
CAGTATATCC AACGTACATC ATCAGATAAG ACATACTCCC TATCAACCAC AGACATAAGA 
GATAGCCATC CATCCTATGG CCATGCTCCA TACCATGCAC AGCAGTACAG TGACGAAGGC 
ACTTTCCATG CTCAAACTAG AACAGCAATG AAACCTCCAT TCAGTGGCTA TTTCAGTAGT 
ATGCAGGAAC TGCACACAAA CAACCACATG CAACTCAACA GCCAGAATCA AACTGGAAGA 
CCAGCTGCAT CACTGTCAGT TAATTTAGGA GTCAATTCCC ACCATATCTC TCAGGGATTA 
ACACAGGCTT CTTTAATGAG AGCTGATGAC TCTAAAGCCT CTTCAGTCTC AGAAATGTCC 
CACAATGGAC GGGACAGAAT GTCCATAGGT TCTCAAGGAG GGAATAAAGA GCGGTATTTC 
CCTCCTCAAT CGCAGCACCA TGATAATAAA GAAAGAAGTG CCTCCTTTAA ACAAAATGAA 
AACCAACATT ATGTTTCTGC ACCAAGTAAT CCCTTGAATA TGTCTGATTC AACAAAACAT 
AATGAGCTTA GGGGATGCCA AAAGCAGCAC CATGTGCATG GCTCAGATGA GCTTTCTGGT 
AGTTATCCAC CCAGTAAGCA GCCCGAGCAC AGGAGAAGTG CTGGCCATCT TCATCATAAG 
GAATACTCGC AACAACCCTT TCCAACTAAA AGCGAATCAA AGATATGTCC CCAGAAAACA 
CCTATGTTGT ATTCTCTGGC CCAAGGGCAC AATGACACAG ATGACTGTTC TAATCAGATC 
AAAAGTGGAA GCGCACAACA TGAAGCCCTT GACAGTCTCA GTAGTAAACA GGTTAGACGG 
AATGACCGAT TTGCCACCAC TTTGCGCAAC GAGATCCAGA TGAGAAGGGC GCATCTCCAA 
AAGAGTCAAA GTGCAGCCAC TTTAGAAAGT CCAGTTGAAA CTGTAGAAGA GTCTGCTTTG 
TGGAAGACCA CAGGGACTGC TTCTCCCTCA TCAGATGGCT GCTTTTCCAT TTCTTATAAA 
GACCATCTTA AAGAAGCTCA GGCCAGAGTC CTCCAGGCTA CATCATTCAG GAGAAAAGAC 
CTAGAGCCAG TATTATTTGA GCATCCAGGT ACTGAAGGTC CCACACGAAA AGATGTACCT 
CCTTCAAGTG TCTCAGAGGT CACACCTAGC AAACCCACCT CAGGGAGTAA TCATGTGCTT 
CGCATTGGAG GCCGTAAGCG GATATCTGCT GAAAAGAAAG TGACGTCTTT TTCTGAGCCA 
GATAAAATTC ATGAAGTAGG AGCTGATGAA CGCTCCACTA TGCCTGAAAA TGTTGCACAC 
TTAGAAAATC AACAAAGATT CTTTGAGACA ACAGGGAAAC CAGTTTTCCC TAAGCCTATG 
CCAAAGCAAA ACCTGCAGAT ATCAGAAGAC CCCAGACTGT CCAAGCCTGG AGGCATGCAC 
TATTCTGCAA ACAGTGATAT GGCAGGGCGG AATAATGGTG AAAGCTCAAC CCCCATTGAA 
CACCACATTA ATGAAGGTCT GGATGGCCCT AACTCAGTGA ACCATCAAGC ATTGCTAGAA 
CAACAGCGAC TAGGCACATT TGCAGAGTAT GAGGCCAAAT GGAACACCCA GAGGAAGACT 
ACAGAACCAA GAGTATCTGG CCGATACCAC TCTGCTGATA ACATCCTCGA TACAGGAAAA 
GAGCGACGGA GTAACCCCAC CTGTGTGCAT GAAAGATCTA GATCCTCACC TTCTGCTGAC 
TTTTATGGGC AGAAACTTCC ACCACAAGAA AAGAAGTCAG CTGACTGTTT CAAATCTGAA 
AAGAGTCCTT GCCAACAGGA CAAGAATAGT ACAAGTCAAA ATTTCTACCC ATCTGTTTCT 
TTGGAGCACA GCCACTGGTT CTATACACCC AGCAGGTTAA ATGAAAAAGG ATATAGTGAA 
ATCTTATGCA AGGAGAAACC AGAAACACCT CCTGAAGAAC CTGAGAAAAA GACTGTAGAT 
CCTCCCTCCA GCCATCATAA GCCTGCACTA CATTTCCCTG TCCATAGCAC TCGCAGTGAA 
CCATCAGCCC TCTCCAAAAA CAAGAGCTCA TTTCTCTTGC CCCATCTGGA GAAGTACAAA 
TGCCCTGAGG GAACACCTCC TCCTCTTAGC AATATTCAGG AGGTCCAGCC TGGATCACAA 
GGAGGAGCTC TGGCCCCAAT TTCCCCAGTC AACAATCAAA GCTCCGCCCC AGTTTCAGCC 
CCCATTCCTT GGAAGAGTCC AGAGTACAGC AAAGGGCCTG TCGGGGAGGA GGAGCAACAC 
CAAGAGCTTG TGCCTCCTCC CTTTCCACCT CCTCCCCCTC CCGTTGTCCT GCCCACCCAA 
CAAACCGCTG GCCCGACCCA GCCCACAATG GATGGGCAGC GTTCGCCCTC GCCCCAGTTC 
GCTCCCCAGA GGCTGACTGA CAAGCCCCCT GTCTCCGTCT CCTCACAGGA TGAAGCTCCT 
GGAAGGATGG ATCAGATTAA AGATGAGAAC ACATCTGTGA AGAAAGTGCC CATTAAGATA 
GTCCATTCAG AGAGTGACAC GGAAAAAGAA AGTCGGCAAT ATCTAGACCT GCCCAGCGAG 
ACGCCTGTCA ACTCGCAGGG ACTTGTGGGA GCTCATCTTC AAAGTTTGGG GAACCCGGAT 
CAGTCATACT CTTTGTTTTG TACATACACC AGACAAAAAG ATCAGGTTCC TGGTCTTAGA 
GACCCAGACA TGGGCCCTCT AAAAGACCAG GGGCCACAAA GCAATATAAA CCCAGTGTCT 
TATGTGGAAC CTGGCCTTCA GACAGGTCCA CCATTGGATC AGAGTACCAA TGGAGTGTCT 
TTGCATCCTT CACATTCAGA GGATGATAAA AGAAAGGAGC TGGCTAGAGA CATAATGGAC 
AAAGACAAGT CCCTCGCTGA CATTTTAGAC CAGAGTAAGA TGAAGACCAC TATGGATCTG 
ATGGAGGGGA TTTTCCCTCA AGGAGATCAG CTACTGGAGG AGGCCCAACA GCGTAGGAAG 
GCTCAACCTA AACCACTCTC CCCTCGAAAC TCTGTTGAGA AGAAAGAGGA AGACAGCCTG 
GTGGCGGCCG CTGCTTTAGT GAGCAACTCA ACCTACTACA GCACATCTGC ACCCAAAGCA 
GAGCTGCTGA TCAAGATGAA GGACATGCAG GACCAGAGCG TTGAACACAA CTCAGAAGAG 
GAGCTGGAGG AGGACAATGA AAGTGACCTC GCCTGCAAGA AGCACGAGTT GATTGAAAGT 
CTCAGTAAGA AGTTGCAGGT CTTAAAAGAA GCTCAGGAGA GCCTGCAGGA GGACGTGCAG 
GACAACAACG CTCTTGGAGA AGAAGTGGAG GCCATCGTAC AGGGCGTCTG CAAGCCTAAT 
GAACTCGACA AATTCCGCAT GTTCGTCGGG GATCTTGATA AAGTGGTCAA CCTTCTGCTG 
TCTCTGTCAG GACGTTTGGC CAGGGTAGAG AATGCCCTGA ACAGCCTGGA GGAAGATGCT 
TCGCTGGAGG AAAGGCGCAC TCTGACAGAG AAACGCAAAC TGCTGATTCG TCAGCACGAG 
GATGCTAAGG AGCTGAAGGA GAACCTGGAC AGGAGAGAGC GTGTGGTCTA CGACATTCTG 
GCCAGTTACC TGCCTGAGGA GAGTATGGCT GACTACGAGC ACTTTGTCAA GATGAAATCC 
GCTCTTATTA TCGAGCAACG CAAGCTGGAG GACAAGATCA AACTGGGAGA TGAGCAGCTC 
AAGTGTCTGA TGGACAGTCT CCCAATGGAC CAGAGAATAA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212127.1
CDS	175..4962
Translation	MKMVDIVAQKMPSESDVHMARSFLTKILRSSMRKNRFKGRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356638.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356638.2

ORF Insert Sequence:

ATGAAAATGG TGGATATTGT AGCGCAGAAA ATGCCATCAG AGAGCGACGT GCATATGGCC 
CGGAGCTTTC TCACGAAGAT TTTACGAAGT TCCATGAGAA AGAATCGGTT TAAAGGGAGA 
AATGAACCCA TGAGCAGACC TCACTCCTGG CATGCCACCA AGTTCAACGA GACCCATTCA 
GAAGCCAAAA CCCAGTCCAC ACCCTCACCA GTTTGGCAAA CAAGATATGA TGCAAGTTCA 
TTCTCTTCTG AACTCTCAAC TGGCTGGGAT CAAACAAATC TACGGAGAGT ATCCGATCAG 
TTCAGTTCTT TGGGGAGTAT GGACAGCCTA GAACATAGTT CTCATCCCTA TCCACCTGGC 
CGTCTTTCCC CAAACAAATC CAACAATAAT AGCATTGAAC ATTTGGGTAG TGGTAAACGG 
GACTCGGCAT ACAGCTCTTT TTCTACAAGT TCGGGAACCC CAGATTACAC ACTGTCCAAA 
AGTAACACGG CATCCACTGA GAACATGCTG TACAAAATAA ATCAGTGGGA TTCAAGTGCC 
AGGCACAGCA ATGGCAGGCA CAGCCAAAAC CTAAGCGAAG GGGTACGACA AGATGAGAGG 
CCTGGATACC TGCACCACCT CTCAAGTACA GGTAACCACG AGATCCCAAG AGCTGAAGAA 
CTGCCTGGCA ACCGTCATTC AAGCTCTGGA AGAGTTAATA TTGGACCTGT CTGGCATGTT 
CCAGATATGA AGAAAAACAT GATATCATCT ACTCCACCTC CCACTCCACC CACACGCAGT 
GATAGCTTTG CAGCTACCAA GGTTCACGAG AAGGGTCTGA TCACAGGTAC TTCAGAGGGC 
CTTGGTGGTC ATTCTCAAGT AAAGCCTCAA GCGAAACCAC AACAAAAGCC AGGAGAAACC 
CATGAAAGCA CACAAAGGTC TAACCAAGTC ATCGAGATTT GTCTTGAGGG TCGACGGGAT 
TATAATCTAC AATCCAAAAA TGATTCCTCA AATAGCTGCA TTTCATCTGA TTCTCATCAT 
CAGTATATCC AACGTACATC ATCAGATAAG ACATACTCCC TATCAACCAC AGACATAAGA 
GATAGCCATC CATCCTATGG CCATGCTCCA TACCATGCAC AGCAGTACAG TGACGAAGGC 
ACTTTCCATG CTCAAACTAG AACAGCAATG AAACCTCCAT TCAGTGGCTA TTTCAGTAGT 
ATGCAGGAAC TGCACACAAA CAACCACATG CAACTCAACA GCCAGAATCA AACTGGAAGA 
CCAGCTGCAT CACTGTCAGT TAATTTAGGA GTCAATTCCC ACCATATCTC TCAGGGATTA 
ACACAGGCTT CTTTAATGAG AGCTGATGAC TCTAAAGCCT CTTCAGTCTC AGAAATGTCC 
CACAATGGAC GGGACAGAAT GTCCATAGGT TCTCAAGGAG GGAATAAAGA GCGGTATTTC 
CCTCCTCAAT CGCAGCACCA TGATAATAAA GAAAGAAGTG CCTCCTTTAA ACAAAATGAA 
AACCAACATT ATGTTTCTGC ACCAAGTAAT CCCTTGAATA TGTCTGATTC AACAAAACAT 
AATGAGCTTA GGGGATGCCA AAAGCAGCAC CATGTGCATG GCTCAGATGA GCTTTCTGGT 
AGTTATCCAC CCAGTAAGCA GCCCGAGCAC AGGAGAAGTG CTGGCCATCT TCATCATAAG 
GAATACTCGC AACAACCCTT TCCAACTAAA AGCGAATCAA AGATATGTCC CCAGAAAACA 
CCTATGTTGT ATTCTCTGGC CCAAGGGCAC AATGACACAG ATGACTGTTC TAATCAGATC 
AAAAGTGGAA GCGCACAACA TGAAGCCCTT GACAGTCTCA GTAGTAAACA GGTTAGACGG 
AATGACCGAT TTGCCACCAC TTTGCGCAAC GAGATCCAGA TGAGAAGGGC GCATCTCCAA 
AAGAGTCAAA GTGCAGCCAC TTTAGAAAGT CCAGTTGAAA CTGTAGAAGA GTCTGCTTTG 
TGGAAGACCA CAGGGACTGC TTCTCCCTCA TCAGATGGCT GCTTTTCCAT TTCTTATAAA 
GACCATCTTA AAGAAGCTCA GGCCAGAGTC CTCCAGGCTA CATCATTCAG GAGAAAAGAC 
CTAGAGCCAG TATTATTTGA GCATCCAGGT ACTGAAGGTC CCACACGAAA AGATGTACCT 
CCTTCAAGTG TCTCAGAGGT CACACCTAGC AAACCCACCT CAGGGAGTAA TCATGTGCTT 
CGCATTGGAG GCCGTAAGCG GATATCTGCT GAAAAGAAAG TGACGTCTTT TTCTGAGCCA 
GATAAAATTC ATGAAGTAGG AGCTGATGAA CGCTCCACTA TGCCTGAAAA TGTTGCACAC 
TTAGAAAATC AACAAAGATT CTTTGAGACA ACAGGGAAAC CAGTTTTCCC TAAGCCTATG 
CCAAAGCAAA ACCTGCAGAT ATCAGAAGAC CCCAGACTGT CCAAGCCTGG AGGCATGCAC 
TATTCTGCAA ACAGTGATAT GGCAGGGCGG AATAATGGTG AAAGCTCAAC CCCCATTGAA 
CACCACATTA ATGAAGGTCT GGATGGCCCT AACTCAGTGA ACCATCAAGC ATTGCTAGAA 
CAACAGCGAC TAGGCACATT TGCAGAGTAT GAGGCCAAAT GGAACACCCA GAGGAAGACT 
ACAGAACCAA GAGTATCTGG CCGATACCAC TCTGCTGATA ACATCCTCGA TACAGGAAAA 
GAGCGACGGA GTAACCCCAC CTGTGTGCAT GAAAGATCTA GATCCTCACC TTCTGCTGAC 
TTTTATGGGC AGAAACTTCC ACCACAAGAA AAGAAGTCAG CTGACTGTTT CAAATCTGAA 
AAGAGTCCTT GCCAACAGGA CAAGAATAGT ACAAGTCAAA ATTTCTACCC ATCTGTTTCT 
TTGGAGCACA GCCACTGGTT CTATACACCC AGCAGGTTAA ATGAAAAAGG ATATAGTGAA 
ATCTTATGCA AGGAGAAACC AGAAACACCT CCTGAAGAAC CTGAGAAAAA GACTGTAGAT 
CCTCCCTCCA GCCATCATAA GCCTGCACTA CATTTCCCTG TCCATAGCAC TCGCAGTGAA 
CCATCAGCCC TCTCCAAAAA CAAGAGCTCA TTTCTCTTGC CCCATCTGGA GAAGTACAAA 
TGCCCTGAGG GAACACCTCC TCCTCTTAGC AATATTCAGG AGGTCCAGCC TGGATCACAA 
GGAGGAGCTC TGGCCCCAAT TTCCCCAGTC AACAATCAAA GCTCCGCCCC AGTTTCAGCC 
CCCATTCCTT GGAAGAGTCC AGAGTACAGC AAAGGGCCTG TCGGGGAGGA GGAGCAACAC 
CAAGAGCTTG TGCCTCCTCC CTTTCCACCT CCTCCCCCTC CCGTTGTCCT GCCCACCCAA 
CAAACCGCTG GCCCGACCCA GCCCACAATG GATGGGCAGC GTTCGCCCTC GCCCCAGTTC 
GCTCCCCAGA GGCTGACTGA CAAGCCCCCT GTCTCCGTCT CCTCACAGGA TGAAGCTCCT 
GGAAGGATGG ATCAGATTAA AGATGAGAAC ACATCTGTGA AGAAAGTGCC CATTAAGATA 
GTCCATTCAG AGAGTGACAC GGAAAAAGAA AGTCGGCAAT ATCTAGACCT GCCCAGCGAG 
ACGCCTGTCA ACTCGCAGGG ACTTGTGGGA GCTCATCTTC AAAGTTTGGG GAACCCGGAT 
CAGTCATACT CTTTGTTTTG TACATACACC AGACAAAAAG ATCAGGTTCC TGGTCTTAGA 
GACCCAGACA TGGGCCCTCT AAAAGACCAG GGGCCACAAA GCAATATAAA CCCAGTGTCT 
TATGTGGAAC CTGGCCTTCA GACAGGTCCA CCATTGGATC AGAGTACCAA TGGAGTGTCT 
TTGCATCCTT CACATTCAGA GGATGATAAA AGAAAGGAGC TGGCTAGAGA CATAATGGAC 
AAAGACAAGT CCCTCGCTGA CATTTTAGAC CAGAGTAAGA TGAAGACCAC TATGGATCTG 
ATGGAGGGGA TTTTCCCTCA AGGAGATCAG CTACTGGAGG AGGCCCAACA GCGTAGGAAG 
GCTCAACCTA AACCACTCTC CCCTCGAAAC TCTGTTGAGA AGAAAGAGGA AGACAGCCTG 
GTGGCGGCCG CTGCTTTAGT GAGCAACTCA ACCTACTACA GCACATCTGC ACCCAAAGCA 
GAGCTGCTGA TCAAGATGAA GGACATGCAG GACCAGAGCG TTGAACACAA CTCAGAAGAG 
GAGCTGGAGG AGGACAATGA AAGTGACCTC GCCTGCAAGA AGCACGAGTT GATTGAAAGT 
CTCAGTAAGA AGTTGCAGGT CTTAAAAGAA GCTCAGGAGA GCCTGCAGGA GGACGTGCAG 
GACAACAACG CTCTTGGAGA AGAAGTGGAG GCCATCGTAC AGGGCGTCTG CAAGCCTAAT 
GAACTCGACA AATTCCGCAT GTTCGTCGGG GATCTTGATA AAGTGGTCAA CCTTCTGCTG 
TCTCTGTCAG GACGTTTGGC CAGGGTAGAG AATGCCCTGA ACAGCCTGGA GGAAGATGCT 
TCGCTGGAGG AAAGGCGCAC TCTGACAGAG AAACGCAAAC TGCTGATTCG TCAGCACGAG 
GATGCTAAGG AGCTGAAGGA GAACCTGGAC AGGAGAGAGC GTGTGGTCTA CGACATTCTG 
GCCAGTTACC TGCCTGAGGA GAGTATGGCT GACTACGAGC ACTTTGTCAA GATGAAATCC 
GCTCTTATTA TCGAGCAACG CAAGCTGGAG GACAAGATCA AACTGGGAGA TGAGCAGCTC 
AAGTGTCTGA TGGACAGTCT CCCAATGGAC CAGAGAATAA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_021477154.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332829.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa59476
Clone ID Related Accession (Same CDS sequence)	XM_017356637.2 , XM_017356637.1 , XM_017356638.1 , XM_017356638.2 , XM_021477154.1
Accession Version	XM_021477154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGG TGGATATTGT AGCGCAGAAA ATGCCATCAG AGAGCGACGT GCATATGGCC 
CGGAGCTTTC TCACGAAGAT TTTACGAAGT TCCATGAGAA AGAATCGGTT TAAAGGGAGA 
AATGAACCCA TGAGCAGACC TCACTCCTGG CATGCCACCA AGTTCAACGA GACCCATTCA 
GAAGCCAAAA CCCAGTCCAC ACCCTCACCA GTTTGGCAAA CAAGATATGA TGCAAGTTCA 
TTCTCTTCTG AACTCTCAAC TGGCTGGGAT CAAACAAATC TACGGAGAGT ATCCGATCAG 
TTCAGTTCTT TGGGGAGTAT GGACAGCCTA GAACATAGTT CTCATCCCTA TCCACCTGGC 
CGTCTTTCCC CAAACAAATC CAACAATAAT AGCATTGAAC ATTTGGGTAG TGGTAAACGG 
GACTCGGCAT ACAGCTCTTT TTCTACAAGT TCGGGAACCC CAGATTACAC ACTGTCCAAA 
AGTAACACGG CATCCACTGA GAACATGCTG TACAAAATAA ATCAGTGGGA TTCAAGTGCC 
AGGCACAGCA ATGGCAGGCA CAGCCAAAAC CTAAGCGAAG GGGTACGACA AGATGAGAGG 
CCTGGATACC TGCACCACCT CTCAAGTACA GGTAACCACG AGATCCCAAG AGCTGAAGAA 
CTGCCTGGCA ACCGTCATTC AAGCTCTGGA AGAGTTAATA TTGGACCTGT CTGGCATGTT 
CCAGATATGA AGAAAAACAT GATATCATCT ACTCCACCTC CCACTCCACC CACACGCAGT 
GATAGCTTTG CAGCTACCAA GGTTCACGAG AAGGGTCTGA TCACAGGTAC TTCAGAGGGC 
CTTGGTGGTC ATTCTCAAGT AAAGCCTCAA GCGAAACCAC AACAAAAGCC AGGAGAAACC 
CATGAAAGCA CACAAAGGTC TAACCAAGTC ATCGAGATTT GTCTTGAGGG TCGACGGGAT 
TATAATCTAC AATCCAAAAA TGATTCCTCA AATAGCTGCA TTTCATCTGA TTCTCATCAT 
CAGTATATCC AACGTACATC ATCAGATAAG ACATACTCCC TATCAACCAC AGACATAAGA 
GATAGCCATC CATCCTATGG CCATGCTCCA TACCATGCAC AGCAGTACAG TGACGAAGGC 
ACTTTCCATG CTCAAACTAG AACAGCAATG AAACCTCCAT TCAGTGGCTA TTTCAGTAGT 
ATGCAGGAAC TGCACACAAA CAACCACATG CAACTCAACA GCCAGAATCA AACTGGAAGA 
CCAGCTGCAT CACTGTCAGT TAATTTAGGA GTCAATTCCC ACCATATCTC TCAGGGATTA 
ACACAGGCTT CTTTAATGAG AGCTGATGAC TCTAAAGCCT CTTCAGTCTC AGAAATGTCC 
CACAATGGAC GGGACAGAAT GTCCATAGGT TCTCAAGGAG GGAATAAAGA GCGGTATTTC 
CCTCCTCAAT CGCAGCACCA TGATAATAAA GAAAGAAGTG CCTCCTTTAA ACAAAATGAA 
AACCAACATT ATGTTTCTGC ACCAAGTAAT CCCTTGAATA TGTCTGATTC AACAAAACAT 
AATGAGCTTA GGGGATGCCA AAAGCAGCAC CATGTGCATG GCTCAGATGA GCTTTCTGGT 
AGTTATCCAC CCAGTAAGCA GCCCGAGCAC AGGAGAAGTG CTGGCCATCT TCATCATAAG 
GAATACTCGC AACAACCCTT TCCAACTAAA AGCGAATCAA AGATATGTCC CCAGAAAACA 
CCTATGTTGT ATTCTCTGGC CCAAGGGCAC AATGACACAG ATGACTGTTC TAATCAGATC 
AAAAGTGGAA GCGCACAACA TGAAGCCCTT GACAGTCTCA GTAGTAAACA GGTTAGACGG 
AATGACCGAT TTGCCACCAC TTTGCGCAAC GAGATCCAGA TGAGAAGGGC GCATCTCCAA 
AAGAGTCAAA GTGCAGCCAC TTTAGAAAGT CCAGTTGAAA CTGTAGAAGA GTCTGCTTTG 
TGGAAGACCA CAGGGACTGC TTCTCCCTCA TCAGATGGCT GCTTTTCCAT TTCTTATAAA 
GACCATCTTA AAGAAGCTCA GGCCAGAGTC CTCCAGGCTA CATCATTCAG GAGAAAAGAC 
CTAGAGCCAG TATTATTTGA GCATCCAGGT ACTGAAGGTC CCACACGAAA AGATGTACCT 
CCTTCAAGTG TCTCAGAGGT CACACCTAGC AAACCCACCT CAGGGAGTAA TCATGTGCTT 
CGCATTGGAG GCCGTAAGCG GATATCTGCT GAAAAGAAAG TGACGTCTTT TTCTGAGCCA 
GATAAAATTC ATGAAGTAGG AGCTGATGAA CGCTCCACTA TGCCTGAAAA TGTTGCACAC 
TTAGAAAATC AACAAAGATT CTTTGAGACA ACAGGGAAAC CAGTTTTCCC TAAGCCTATG 
CCAAAGCAAA ACCTGCAGAT ATCAGAAGAC CCCAGACTGT CCAAGCCTGG AGGCATGCAC 
TATTCTGCAA ACAGTGATAT GGCAGGGCGG AATAATGGTG AAAGCTCAAC CCCCATTGAA 
CACCACATTA ATGAAGGTCT GGATGGCCCT AACTCAGTGA ACCATCAAGC ATTGCTAGAA 
CAACAGCGAC TAGGCACATT TGCAGAGTAT GAGGCCAAAT GGAACACCCA GAGGAAGACT 
ACAGAACCAA GAGTATCTGG CCGATACCAC TCTGCTGATA ACATCCTCGA TACAGGAAAA 
GAGCGACGGA GTAACCCCAC CTGTGTGCAT GAAAGATCTA GATCCTCACC TTCTGCTGAC 
TTTTATGGGC AGAAACTTCC ACCACAAGAA AAGAAGTCAG CTGACTGTTT CAAATCTGAA 
AAGAGTCCTT GCCAACAGGA CAAGAATAGT ACAAGTCAAA ATTTCTACCC ATCTGTTTCT 
TTGGAGCACA GCCACTGGTT CTATACACCC AGCAGGTTAA ATGAAAAAGG ATATAGTGAA 
ATCTTATGCA AGGAGAAACC AGAAACACCT CCTGAAGAAC CTGAGAAAAA GACTGTAGAT 
CCTCCCTCCA GCCATCATAA GCCTGCACTA CATTTCCCTG TCCATAGCAC TCGCAGTGAA 
CCATCAGCCC TCTCCAAAAA CAAGAGCTCA TTTCTCTTGC CCCATCTGGA GAAGTACAAA 
TGCCCTGAGG GAACACCTCC TCCTCTTAGC AATATTCAGG AGGTCCAGCC TGGATCACAA 
GGAGGAGCTC TGGCCCCAAT TTCCCCAGTC AACAATCAAA GCTCCGCCCC AGTTTCAGCC 
CCCATTCCTT GGAAGAGTCC AGAGTACAGC AAAGGGCCTG TCGGGGAGGA GGAGCAACAC 
CAAGAGCTTG TGCCTCCTCC CTTTCCACCT CCTCCCCCTC CCGTTGTCCT GCCCACCCAA 
CAAACCGCTG GCCCGACCCA GCCCACAATG GATGGGCAGC GTTCGCCCTC GCCCCAGTTC 
GCTCCCCAGA GGCTGACTGA CAAGCCCCCT GTCTCCGTCT CCTCACAGGA TGAAGCTCCT 
GGAAGGATGG ATCAGATTAA AGATGAGAAC ACATCTGTGA AGAAAGTGCC CATTAAGATA 
GTCCATTCAG AGAGTGACAC GGAAAAAGAA AGTCGGCAAT ATCTAGACCT GCCCAGCGAG 
ACGCCTGTCA ACTCGCAGGG ACTTGTGGGA GCTCATCTTC AAAGTTTGGG GAACCCGGAT 
CAGTCATACT CTTTGTTTTG TACATACACC AGACAAAAAG ATCAGGTTCC TGGTCTTAGA 
GACCCAGACA TGGGCCCTCT AAAAGACCAG GGGCCACAAA GCAATATAAA CCCAGTGTCT 
TATGTGGAAC CTGGCCTTCA GACAGGTCCA CCATTGGATC AGAGTACCAA TGGAGTGTCT 
TTGCATCCTT CACATTCAGA GGATGATAAA AGAAAGGAGC TGGCTAGAGA CATAATGGAC 
AAAGACAAGT CCCTCGCTGA CATTTTAGAC CAGAGTAAGA TGAAGACCAC TATGGATCTG 
ATGGAGGGGA TTTTCCCTCA AGGAGATCAG CTACTGGAGG AGGCCCAACA GCGTAGGAAG 
GCTCAACCTA AACCACTCTC CCCTCGAAAC TCTGTTGAGA AGAAAGAGGA AGACAGCCTG 
GTGGCGGCCG CTGCTTTAGT GAGCAACTCA ACCTACTACA GCACATCTGC ACCCAAAGCA 
GAGCTGCTGA TCAAGATGAA GGACATGCAG GACCAGAGCG TTGAACACAA CTCAGAAGAG 
GAGCTGGAGG AGGACAATGA AAGTGACCTC GCCTGCAAGA AGCACGAGTT GATTGAAAGT 
CTCAGTAAGA AGTTGCAGGT CTTAAAAGAA GCTCAGGAGA GCCTGCAGGA GGACGTGCAG 
GACAACAACG CTCTTGGAGA AGAAGTGGAG GCCATCGTAC AGGGCGTCTG CAAGCCTAAT 
GAACTCGACA AATTCCGCAT GTTCGTCGGG GATCTTGATA AAGTGGTCAA CCTTCTGCTG 
TCTCTGTCAG GACGTTTGGC CAGGGTAGAG AATGCCCTGA ACAGCCTGGA GGAAGATGCT 
TCGCTGGAGG AAAGGCGCAC TCTGACAGAG AAACGCAAAC TGCTGATTCG TCAGCACGAG 
GATGCTAAGG AGCTGAAGGA GAACCTGGAC AGGAGAGAGC GTGTGGTCTA CGACATTCTG 
GCCAGTTACC TGCCTGAGGA GAGTATGGCT GACTACGAGC ACTTTGTCAA GATGAAATCC 
GCTCTTATTA TCGAGCAACG CAAGCTGGAG GACAAGATCA AACTGGGAGA TGAGCAGCTC 
AAGTGTCTGA TGGACAGTCT CCCAATGGAC CAGAGAATAA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332829.1
CDS	283..5070
Translation	MKMVDIVAQKMPSESDVHMARSFLTKILRSSMRKNRFKGRNEPMSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_021477154.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477154.1

ORF Insert Sequence:

ATGAAAATGG TGGATATTGT AGCGCAGAAA ATGCCATCAG AGAGCGACGT GCATATGGCC 
CGGAGCTTTC TCACGAAGAT TTTACGAAGT TCCATGAGAA AGAATCGGTT TAAAGGGAGA 
AATGAACCCA TGAGCAGACC TCACTCCTGG CATGCCACCA AGTTCAACGA GACCCATTCA 
GAAGCCAAAA CCCAGTCCAC ACCCTCACCA GTTTGGCAAA CAAGATATGA TGCAAGTTCA 
TTCTCTTCTG AACTCTCAAC TGGCTGGGAT CAAACAAATC TACGGAGAGT ATCCGATCAG 
TTCAGTTCTT TGGGGAGTAT GGACAGCCTA GAACATAGTT CTCATCCCTA TCCACCTGGC 
CGTCTTTCCC CAAACAAATC CAACAATAAT AGCATTGAAC ATTTGGGTAG TGGTAAACGG 
GACTCGGCAT ACAGCTCTTT TTCTACAAGT TCGGGAACCC CAGATTACAC ACTGTCCAAA 
AGTAACACGG CATCCACTGA GAACATGCTG TACAAAATAA ATCAGTGGGA TTCAAGTGCC 
AGGCACAGCA ATGGCAGGCA CAGCCAAAAC CTAAGCGAAG GGGTACGACA AGATGAGAGG 
CCTGGATACC TGCACCACCT CTCAAGTACA GGTAACCACG AGATCCCAAG AGCTGAAGAA 
CTGCCTGGCA ACCGTCATTC AAGCTCTGGA AGAGTTAATA TTGGACCTGT CTGGCATGTT 
CCAGATATGA AGAAAAACAT GATATCATCT ACTCCACCTC CCACTCCACC CACACGCAGT 
GATAGCTTTG CAGCTACCAA GGTTCACGAG AAGGGTCTGA TCACAGGTAC TTCAGAGGGC 
CTTGGTGGTC ATTCTCAAGT AAAGCCTCAA GCGAAACCAC AACAAAAGCC AGGAGAAACC 
CATGAAAGCA CACAAAGGTC TAACCAAGTC ATCGAGATTT GTCTTGAGGG TCGACGGGAT 
TATAATCTAC AATCCAAAAA TGATTCCTCA AATAGCTGCA TTTCATCTGA TTCTCATCAT 
CAGTATATCC AACGTACATC ATCAGATAAG ACATACTCCC TATCAACCAC AGACATAAGA 
GATAGCCATC CATCCTATGG CCATGCTCCA TACCATGCAC AGCAGTACAG TGACGAAGGC 
ACTTTCCATG CTCAAACTAG AACAGCAATG AAACCTCCAT TCAGTGGCTA TTTCAGTAGT 
ATGCAGGAAC TGCACACAAA CAACCACATG CAACTCAACA GCCAGAATCA AACTGGAAGA 
CCAGCTGCAT CACTGTCAGT TAATTTAGGA GTCAATTCCC ACCATATCTC TCAGGGATTA 
ACACAGGCTT CTTTAATGAG AGCTGATGAC TCTAAAGCCT CTTCAGTCTC AGAAATGTCC 
CACAATGGAC GGGACAGAAT GTCCATAGGT TCTCAAGGAG GGAATAAAGA GCGGTATTTC 
CCTCCTCAAT CGCAGCACCA TGATAATAAA GAAAGAAGTG CCTCCTTTAA ACAAAATGAA 
AACCAACATT ATGTTTCTGC ACCAAGTAAT CCCTTGAATA TGTCTGATTC AACAAAACAT 
AATGAGCTTA GGGGATGCCA AAAGCAGCAC CATGTGCATG GCTCAGATGA GCTTTCTGGT 
AGTTATCCAC CCAGTAAGCA GCCCGAGCAC AGGAGAAGTG CTGGCCATCT TCATCATAAG 
GAATACTCGC AACAACCCTT TCCAACTAAA AGCGAATCAA AGATATGTCC CCAGAAAACA 
CCTATGTTGT ATTCTCTGGC CCAAGGGCAC AATGACACAG ATGACTGTTC TAATCAGATC 
AAAAGTGGAA GCGCACAACA TGAAGCCCTT GACAGTCTCA GTAGTAAACA GGTTAGACGG 
AATGACCGAT TTGCCACCAC TTTGCGCAAC GAGATCCAGA TGAGAAGGGC GCATCTCCAA 
AAGAGTCAAA GTGCAGCCAC TTTAGAAAGT CCAGTTGAAA CTGTAGAAGA GTCTGCTTTG 
TGGAAGACCA CAGGGACTGC TTCTCCCTCA TCAGATGGCT GCTTTTCCAT TTCTTATAAA 
GACCATCTTA AAGAAGCTCA GGCCAGAGTC CTCCAGGCTA CATCATTCAG GAGAAAAGAC 
CTAGAGCCAG TATTATTTGA GCATCCAGGT ACTGAAGGTC CCACACGAAA AGATGTACCT 
CCTTCAAGTG TCTCAGAGGT CACACCTAGC AAACCCACCT CAGGGAGTAA TCATGTGCTT 
CGCATTGGAG GCCGTAAGCG GATATCTGCT GAAAAGAAAG TGACGTCTTT TTCTGAGCCA 
GATAAAATTC ATGAAGTAGG AGCTGATGAA CGCTCCACTA TGCCTGAAAA TGTTGCACAC 
TTAGAAAATC AACAAAGATT CTTTGAGACA ACAGGGAAAC CAGTTTTCCC TAAGCCTATG 
CCAAAGCAAA ACCTGCAGAT ATCAGAAGAC CCCAGACTGT CCAAGCCTGG AGGCATGCAC 
TATTCTGCAA ACAGTGATAT GGCAGGGCGG AATAATGGTG AAAGCTCAAC CCCCATTGAA 
CACCACATTA ATGAAGGTCT GGATGGCCCT AACTCAGTGA ACCATCAAGC ATTGCTAGAA 
CAACAGCGAC TAGGCACATT TGCAGAGTAT GAGGCCAAAT GGAACACCCA GAGGAAGACT 
ACAGAACCAA GAGTATCTGG CCGATACCAC TCTGCTGATA ACATCCTCGA TACAGGAAAA 
GAGCGACGGA GTAACCCCAC CTGTGTGCAT GAAAGATCTA GATCCTCACC TTCTGCTGAC 
TTTTATGGGC AGAAACTTCC ACCACAAGAA AAGAAGTCAG CTGACTGTTT CAAATCTGAA 
AAGAGTCCTT GCCAACAGGA CAAGAATAGT ACAAGTCAAA ATTTCTACCC ATCTGTTTCT 
TTGGAGCACA GCCACTGGTT CTATACACCC AGCAGGTTAA ATGAAAAAGG ATATAGTGAA 
ATCTTATGCA AGGAGAAACC AGAAACACCT CCTGAAGAAC CTGAGAAAAA GACTGTAGAT 
CCTCCCTCCA GCCATCATAA GCCTGCACTA CATTTCCCTG TCCATAGCAC TCGCAGTGAA 
CCATCAGCCC TCTCCAAAAA CAAGAGCTCA TTTCTCTTGC CCCATCTGGA GAAGTACAAA 
TGCCCTGAGG GAACACCTCC TCCTCTTAGC AATATTCAGG AGGTCCAGCC TGGATCACAA 
GGAGGAGCTC TGGCCCCAAT TTCCCCAGTC AACAATCAAA GCTCCGCCCC AGTTTCAGCC 
CCCATTCCTT GGAAGAGTCC AGAGTACAGC AAAGGGCCTG TCGGGGAGGA GGAGCAACAC 
CAAGAGCTTG TGCCTCCTCC CTTTCCACCT CCTCCCCCTC CCGTTGTCCT GCCCACCCAA 
CAAACCGCTG GCCCGACCCA GCCCACAATG GATGGGCAGC GTTCGCCCTC GCCCCAGTTC 
GCTCCCCAGA GGCTGACTGA CAAGCCCCCT GTCTCCGTCT CCTCACAGGA TGAAGCTCCT 
GGAAGGATGG ATCAGATTAA AGATGAGAAC ACATCTGTGA AGAAAGTGCC CATTAAGATA 
GTCCATTCAG AGAGTGACAC GGAAAAAGAA AGTCGGCAAT ATCTAGACCT GCCCAGCGAG 
ACGCCTGTCA ACTCGCAGGG ACTTGTGGGA GCTCATCTTC AAAGTTTGGG GAACCCGGAT 
CAGTCATACT CTTTGTTTTG TACATACACC AGACAAAAAG ATCAGGTTCC TGGTCTTAGA 
GACCCAGACA TGGGCCCTCT AAAAGACCAG GGGCCACAAA GCAATATAAA CCCAGTGTCT 
TATGTGGAAC CTGGCCTTCA GACAGGTCCA CCATTGGATC AGAGTACCAA TGGAGTGTCT 
TTGCATCCTT CACATTCAGA GGATGATAAA AGAAAGGAGC TGGCTAGAGA CATAATGGAC 
AAAGACAAGT CCCTCGCTGA CATTTTAGAC CAGAGTAAGA TGAAGACCAC TATGGATCTG 
ATGGAGGGGA TTTTCCCTCA AGGAGATCAG CTACTGGAGG AGGCCCAACA GCGTAGGAAG 
GCTCAACCTA AACCACTCTC CCCTCGAAAC TCTGTTGAGA AGAAAGAGGA AGACAGCCTG 
GTGGCGGCCG CTGCTTTAGT GAGCAACTCA ACCTACTACA GCACATCTGC ACCCAAAGCA 
GAGCTGCTGA TCAAGATGAA GGACATGCAG GACCAGAGCG TTGAACACAA CTCAGAAGAG 
GAGCTGGAGG AGGACAATGA AAGTGACCTC GCCTGCAAGA AGCACGAGTT GATTGAAAGT 
CTCAGTAAGA AGTTGCAGGT CTTAAAAGAA GCTCAGGAGA GCCTGCAGGA GGACGTGCAG 
GACAACAACG CTCTTGGAGA AGAAGTGGAG GCCATCGTAC AGGGCGTCTG CAAGCCTAAT 
GAACTCGACA AATTCCGCAT GTTCGTCGGG GATCTTGATA AAGTGGTCAA CCTTCTGCTG 
TCTCTGTCAG GACGTTTGGC CAGGGTAGAG AATGCCCTGA ACAGCCTGGA GGAAGATGCT 
TCGCTGGAGG AAAGGCGCAC TCTGACAGAG AAACGCAAAC TGCTGATTCG TCAGCACGAG 
GATGCTAAGG AGCTGAAGGA GAACCTGGAC AGGAGAGAGC GTGTGGTCTA CGACATTCTG 
GCCAGTTACC TGCCTGAGGA GAGTATGGCT GACTACGAGC ACTTTGTCAA GATGAAATCC 
GCTCTTATTA TCGAGCAACG CAAGCTGGAG GACAAGATCA AACTGGGAGA TGAGCAGCTC 
AAGTGTCTGA TGGACAGTCT CCCAATGGAC CAGAGAATAA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356639.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212128.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X8, mRNA.

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CloneID	ODa59477
Clone ID Related Accession (Same CDS sequence)	XM_017356639.1 , XM_017356639.2
Accession Version	XM_017356639.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom2 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGAC CTCACTCCTG GCATGCCACC AAGTTCAACG AGACCCATTC AGAAGCCAAA 
ACCCAGTCCA CACCCTCACC AGTTTGGCAA ACAAGATATG ATGCAAGTTC ATTCTCTTCT 
GAACTCTCAA CTGGCTGGGA TCAAACAAAT CTACGGAGAG TATCCGATCA GTTCAGTTCT 
TTGGGGAGTA TGGACAGCCT AGAACATAGT TCTCATCCCT ATCCACCTGG CCGTCTTTCC 
CCAAACAAAT CCAACAATAA TAGCATTGAA CATTTGGGTA GTGGTAAACG GGACTCGGCA 
TACAGCTCTT TTTCTACAAG TTCGGGAACC CCAGATTACA CACTGTCCAA AAGTAACACG 
GCATCCACTG AGAACATGCT GTACAAAATA AATCAGTGGG ATTCAAGTGC CAGGCACAGC 
AATGGCAGGC ACAGCCAAAA CCTAAGCGAA GGGGTACGAC AAGATGAGAG GCCTGGATAC 
CTGCACCACC TCTCAAGTAC AGGTAACCAC GAGATCCCAA GAGCTGAAGA ACTGCCTGGC 
AACCGTCATT CAAGCTCTGG AAGAGTTAAT ATTGGACCTG TCTGGCATGT TCCAGATATG 
AAGAAAAACA TGATATCATC TACTCCACCT CCCACTCCAC CCACACGCAG TGATAGCTTT 
GCAGCTACCA AGGTTCACGA GAAGGGTCTG ATCACAGGTA CTTCAGAGGG CCTTGGTGGT 
CATTCTCAAG TAAAGCCTCA AGCGAAACCA CAACAAAAGC CAGGAGAAAC CCATGAAAGC 
ACACAAAGGT CTAACCAAGT CATCGAGATT TGTCTTGAGG GTCGACGGGA TTATAATCTA 
CAATCCAAAA ATGATTCCTC AAATAGCTGC ATTTCATCTG ATTCTCATCA TCAGTATATC 
CAACGTACAT CATCAGATAA GACATACTCC CTATCAACCA CAGACATAAG AGATAGCCAT 
CCATCCTATG GCCATGCTCC ATACCATGCA CAGCAGTACA GTGACGAAGG CACTTTCCAT 
GCTCAAACTA GAACAGCAAT GAAACCTCCA TTCAGTGGCT ATTTCAGTAG TATGCAGGAA 
CTGCACACAA ACAACCACAT GCAACTCAAC AGCCAGAATC AAACTGGAAG ACCAGCTGCA 
TCACTGTCAG TTAATTTAGG AGTCAATTCC CACCATATCT CTCAGGGATT AACACAGGCT 
TCTTTAATGA GAGCTGATGA CTCTAAAGCC TCTTCAGTCT CAGAAATGTC CCACAATGGA 
CGGGACAGAA TGTCCATAGG TTCTCAAGGA GGGAATAAAG AGCGGTATTT CCCTCCTCAA 
TCGCAGCACC ATGATAATAA AGAAAGAAGT GCCTCCTTTA AACAAAATGA AAACCAACAT 
TATGTTTCTG CACCAAGTAA TCCCTTGAAT ATGTCTGATT CAACAAAACA TAATGAGCTT 
AGGGGATGCC AAAAGCAGCA CCATGTGCAT GGCTCAGATG AGCTTTCTGG TAGTTATCCA 
CCCAGTAAGC AGCCCGAGCA CAGGAGAAGT GCTGGCCATC TTCATCATAA GGAATACTCG 
CAACAACCCT TTCCAACTAA AAGCGAATCA AAGATATGTC CCCAGAAAAC ACCTATGTTG 
TATTCTCTGG CCCAAGGGCA CAATGACACA GATGACTGTT CTAATCAGAT CAAAAGTGGA 
AGCGCACAAC ATGAAGCCCT TGACAGTCTC AGTAGTAAAC AGGTTAGACG GAATGACCGA 
TTTGCCACCA CTTTGCGCAA CGAGATCCAG ATGAGAAGGG CGCATCTCCA AAAGAGTCAA 
AGTGCAGCCA CTTTAGAAAG TCCAGTTGAA ACTGTAGAAG AGTCTGCTTT GTGGAAGACC 
ACAGGGACTG CTTCTCCCTC ATCAGATGGC TGCTTTTCCA TTTCTTATAA AGACCATCTT 
AAAGAAGCTC AGGCCAGAGT CCTCCAGGCT ACATCATTCA GGAGAAAAGA CCTAGAGCCA 
GTATTATTTG AGCATCCAGG TACTGAAGGT CCCACACGAA AAGATGTACC TCCTTCAAGT 
GTCTCAGAGG TCACACCTAG CAAACCCACC TCAGGGAGTA ATCATGTGCT TCGCATTGGA 
GGCCGTAAGC GGATATCTGC TGAAAAGAAA GTGACGTCTT TTTCTGAGCC AGATAAAATT 
CATGAAGTAG GAGCTGATGA ACGCTCCACT ATGCCTGAAA ATGTTGCACA CTTAGAAAAT 
CAACAAAGAT TCTTTGAGAC AACAGGGAAA CCAGTTTTCC CTAAGCCTAT GCCAAAGCAA 
AACCTGCAGA TATCAGAAGA CCCCAGACTG TCCAAGCCTG GAGGCATGCA CTATTCTGCA 
AACAGTGATA TGGCAGGGCG GAATAATGGT GAAAGCTCAA CCCCCATTGA ACACCACATT 
AATGAAGGTC TGGATGGCCC TAACTCAGTG AACCATCAAG CATTGCTAGA ACAACAGCGA 
CTAGGCACAT TTGCAGAGTA TGAGGCCAAA TGGAACACCC AGAGGAAGAC TACAGAACCA 
AGAGTATCTG GCCGATACCA CTCTGCTGAT AACATCCTCG ATACAGGAAA AGAGCGACGG 
AGTAACCCCA CCTGTGTGCA TGAAAGATCT AGATCCTCAC CTTCTGCTGA CTTTTATGGG 
CAGAAACTTC CACCACAAGA AAAGAAGTCA GCTGACTGTT TCAAATCTGA AAAGAGTCCT 
TGCCAACAGG ACAAGAATAG TACAAGTCAA AATTTCTACC CATCTGTTTC TTTGGAGCAC 
AGCCACTGGT TCTATACACC CAGCAGGTTA AATGAAAAAG GATATAGTGA AATCTTATGC 
AAGGAGAAAC CAGAAACACC TCCTGAAGAA CCTGAGAAAA AGACTGTAGA TCCTCCCTCC 
AGCCATCATA AGCCTGCACT ACATTTCCCT GTCCATAGCA CTCGCAGTGA ACCATCAGCC 
CTCTCCAAAA ACAAGAGCTC ATTTCTCTTG CCCCATCTGG AGAAGTACAA ATGCCCTGAG 
GGAACACCTC CTCCTCTTAG CAATATTCAG GAGGTCCAGC CTGGATCACA AGGAGGAGCT 
CTGGCCCCAA TTTCCCCAGT CAACAATCAA AGCTCCGCCC CAGTTTCAGC CCCCATTCCT 
TGGAAGAGTC CAGAGTACAG CAAAGGGCCT GTCGGGGAGG AGGAGCAACA CCAAGAGCTT 
GTGCCTCCTC CCTTTCCACC TCCTCCCCCT CCCGTTGTCC TGCCCACCCA ACAAACCGCT 
GGCCCGACCC AGCCCACAAT GGATGGGCAG CGTTCGCCCT CGCCCCAGTT CGCTCCCCAG 
AGGCTGACTG ACAAGCCCCC TGTCTCCGTC TCCTCACAGG ATGAAGCTCC TGGAAGGATG 
GATCAGATTA AAGATGAGAA CACATCTGTG AAGAAAGTGC CCATTAAGAT AGTCCATTCA 
GAGAGTGACA CGGAAAAAGA AAGTCGGCAA TATCTAGACC TGCCCAGCGA GACGCCTGTC 
AACTCGCAGG GACTTGTGGG AGCTCATCTT CAAAGTTTGG GGAACCCGGA TCAGTCATAC 
TCTTTGTTTT GTACATACAC CAGACAAAAA GATCAGGTTC CTGGTCTTAG AGACCCAGAC 
ATGGGCCCTC TAAAAGACCA GGGGCCACAA AGCAATATAA ACCCAGTGTC TTATGTGGAA 
CCTGGCCTTC AGACAGGTCC ACCATTGGAT CAGAGTACCA ATGGAGTGTC TTTGCATCCT 
TCACATTCAG AGGATGATAA AAGAAAGGAG CTGGCTAGAG ACATAATGGA CAAAGACAAG 
TCCCTCGCTG ACATTTTAGA CCAGAGTAAG ATGAAGACCA CTATGGATCT GATGGAGGGG 
ATTTTCCCTC AAGGAGATCA GCTACTGGAG GAGGCCCAAC AGCGTAGGAA GGCTCAACCT 
AAACCACTCT CCCCTCGAAA CTCTGTTGAG AAGAAAGAGG AAGACAGCCT GGTGGCGGCC 
GCTGCTTTAG TGAGCAACTC AACCTACTAC AGCACATCTG CACCCAAAGC AGAGCTGCTG 
ATCAAGATGA AGGACATGCA GGACCAGAGC GTTGAACACA ACTCAGAAGA GGAGCTGGAG 
GAGGACAATG AAAGTGACCT CGCCTGCAAG AAGCACGAGT TGATTGAAAG TCTCAGTAAG 
AAGTTGCAGG TCTTAAAAGA AGCTCAGGAG AGCCTGCAGG AGGACGTGCA GGACAACAAC 
GCTCTTGGAG AAGAAGTGGA GGCCATCGTA CAGGGCGTCT GCAAGCCTAA TGAACTCGAC 
AAATTCCGCA TGTTCGTCGG GGATCTTGAT AAAGTGGTCA ACCTTCTGCT GTCTCTGTCA 
GGACGTTTGG CCAGGGTAGA GAATGCCCTG AACAGCCTGG AGGAAGATGC TTCGCTGGAG 
GAAAGGCGCA CTCTGACAGA GAAACGCAAA CTGCTGATTC GTCAGCACGA GGATGCTAAG 
GAGCTGAAGG AGAACCTGGA CAGGAGAGAG CGTGTGGTCT ACGACATTCT GGCCAGTTAC 
CTGCCTGAGG AGAGTATGGC TGACTACGAG CACTTTGTCA AGATGAAATC CGCTCTTATT 
ATCGAGCAAC GCAAGCTGGA GGACAAGATC AAACTGGGAG ATGAGCAGCT CAAGTGTCTG 
ATGGACAGTC TCCCAATGGA CCAGAGAATA ATCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212128.1
CDS	866..5524
Translation	MSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356639.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356639.1

ORF Insert Sequence:

ATGAGCAGAC CTCACTCCTG GCATGCCACC AAGTTCAACG AGACCCATTC AGAAGCCAAA 
ACCCAGTCCA CACCCTCACC AGTTTGGCAA ACAAGATATG ATGCAAGTTC ATTCTCTTCT 
GAACTCTCAA CTGGCTGGGA TCAAACAAAT CTACGGAGAG TATCCGATCA GTTCAGTTCT 
TTGGGGAGTA TGGACAGCCT AGAACATAGT TCTCATCCCT ATCCACCTGG CCGTCTTTCC 
CCAAACAAAT CCAACAATAA TAGCATTGAA CATTTGGGTA GTGGTAAACG GGACTCGGCA 
TACAGCTCTT TTTCTACAAG TTCGGGAACC CCAGATTACA CACTGTCCAA AAGTAACACG 
GCATCCACTG AGAACATGCT GTACAAAATA AATCAGTGGG ATTCAAGTGC CAGGCACAGC 
AATGGCAGGC ACAGCCAAAA CCTAAGCGAA GGGGTACGAC AAGATGAGAG GCCTGGATAC 
CTGCACCACC TCTCAAGTAC AGGTAACCAC GAGATCCCAA GAGCTGAAGA ACTGCCTGGC 
AACCGTCATT CAAGCTCTGG AAGAGTTAAT ATTGGACCTG TCTGGCATGT TCCAGATATG 
AAGAAAAACA TGATATCATC TACTCCACCT CCCACTCCAC CCACACGCAG TGATAGCTTT 
GCAGCTACCA AGGTTCACGA GAAGGGTCTG ATCACAGGTA CTTCAGAGGG CCTTGGTGGT 
CATTCTCAAG TAAAGCCTCA AGCGAAACCA CAACAAAAGC CAGGAGAAAC CCATGAAAGC 
ACACAAAGGT CTAACCAAGT CATCGAGATT TGTCTTGAGG GTCGACGGGA TTATAATCTA 
CAATCCAAAA ATGATTCCTC AAATAGCTGC ATTTCATCTG ATTCTCATCA TCAGTATATC 
CAACGTACAT CATCAGATAA GACATACTCC CTATCAACCA CAGACATAAG AGATAGCCAT 
CCATCCTATG GCCATGCTCC ATACCATGCA CAGCAGTACA GTGACGAAGG CACTTTCCAT 
GCTCAAACTA GAACAGCAAT GAAACCTCCA TTCAGTGGCT ATTTCAGTAG TATGCAGGAA 
CTGCACACAA ACAACCACAT GCAACTCAAC AGCCAGAATC AAACTGGAAG ACCAGCTGCA 
TCACTGTCAG TTAATTTAGG AGTCAATTCC CACCATATCT CTCAGGGATT AACACAGGCT 
TCTTTAATGA GAGCTGATGA CTCTAAAGCC TCTTCAGTCT CAGAAATGTC CCACAATGGA 
CGGGACAGAA TGTCCATAGG TTCTCAAGGA GGGAATAAAG AGCGGTATTT CCCTCCTCAA 
TCGCAGCACC ATGATAATAA AGAAAGAAGT GCCTCCTTTA AACAAAATGA AAACCAACAT 
TATGTTTCTG CACCAAGTAA TCCCTTGAAT ATGTCTGATT CAACAAAACA TAATGAGCTT 
AGGGGATGCC AAAAGCAGCA CCATGTGCAT GGCTCAGATG AGCTTTCTGG TAGTTATCCA 
CCCAGTAAGC AGCCCGAGCA CAGGAGAAGT GCTGGCCATC TTCATCATAA GGAATACTCG 
CAACAACCCT TTCCAACTAA AAGCGAATCA AAGATATGTC CCCAGAAAAC ACCTATGTTG 
TATTCTCTGG CCCAAGGGCA CAATGACACA GATGACTGTT CTAATCAGAT CAAAAGTGGA 
AGCGCACAAC ATGAAGCCCT TGACAGTCTC AGTAGTAAAC AGGTTAGACG GAATGACCGA 
TTTGCCACCA CTTTGCGCAA CGAGATCCAG ATGAGAAGGG CGCATCTCCA AAAGAGTCAA 
AGTGCAGCCA CTTTAGAAAG TCCAGTTGAA ACTGTAGAAG AGTCTGCTTT GTGGAAGACC 
ACAGGGACTG CTTCTCCCTC ATCAGATGGC TGCTTTTCCA TTTCTTATAA AGACCATCTT 
AAAGAAGCTC AGGCCAGAGT CCTCCAGGCT ACATCATTCA GGAGAAAAGA CCTAGAGCCA 
GTATTATTTG AGCATCCAGG TACTGAAGGT CCCACACGAA AAGATGTACC TCCTTCAAGT 
GTCTCAGAGG TCACACCTAG CAAACCCACC TCAGGGAGTA ATCATGTGCT TCGCATTGGA 
GGCCGTAAGC GGATATCTGC TGAAAAGAAA GTGACGTCTT TTTCTGAGCC AGATAAAATT 
CATGAAGTAG GAGCTGATGA ACGCTCCACT ATGCCTGAAA ATGTTGCACA CTTAGAAAAT 
CAACAAAGAT TCTTTGAGAC AACAGGGAAA CCAGTTTTCC CTAAGCCTAT GCCAAAGCAA 
AACCTGCAGA TATCAGAAGA CCCCAGACTG TCCAAGCCTG GAGGCATGCA CTATTCTGCA 
AACAGTGATA TGGCAGGGCG GAATAATGGT GAAAGCTCAA CCCCCATTGA ACACCACATT 
AATGAAGGTC TGGATGGCCC TAACTCAGTG AACCATCAAG CATTGCTAGA ACAACAGCGA 
CTAGGCACAT TTGCAGAGTA TGAGGCCAAA TGGAACACCC AGAGGAAGAC TACAGAACCA 
AGAGTATCTG GCCGATACCA CTCTGCTGAT AACATCCTCG ATACAGGAAA AGAGCGACGG 
AGTAACCCCA CCTGTGTGCA TGAAAGATCT AGATCCTCAC CTTCTGCTGA CTTTTATGGG 
CAGAAACTTC CACCACAAGA AAAGAAGTCA GCTGACTGTT TCAAATCTGA AAAGAGTCCT 
TGCCAACAGG ACAAGAATAG TACAAGTCAA AATTTCTACC CATCTGTTTC TTTGGAGCAC 
AGCCACTGGT TCTATACACC CAGCAGGTTA AATGAAAAAG GATATAGTGA AATCTTATGC 
AAGGAGAAAC CAGAAACACC TCCTGAAGAA CCTGAGAAAA AGACTGTAGA TCCTCCCTCC 
AGCCATCATA AGCCTGCACT ACATTTCCCT GTCCATAGCA CTCGCAGTGA ACCATCAGCC 
CTCTCCAAAA ACAAGAGCTC ATTTCTCTTG CCCCATCTGG AGAAGTACAA ATGCCCTGAG 
GGAACACCTC CTCCTCTTAG CAATATTCAG GAGGTCCAGC CTGGATCACA AGGAGGAGCT 
CTGGCCCCAA TTTCCCCAGT CAACAATCAA AGCTCCGCCC CAGTTTCAGC CCCCATTCCT 
TGGAAGAGTC CAGAGTACAG CAAAGGGCCT GTCGGGGAGG AGGAGCAACA CCAAGAGCTT 
GTGCCTCCTC CCTTTCCACC TCCTCCCCCT CCCGTTGTCC TGCCCACCCA ACAAACCGCT 
GGCCCGACCC AGCCCACAAT GGATGGGCAG CGTTCGCCCT CGCCCCAGTT CGCTCCCCAG 
AGGCTGACTG ACAAGCCCCC TGTCTCCGTC TCCTCACAGG ATGAAGCTCC TGGAAGGATG 
GATCAGATTA AAGATGAGAA CACATCTGTG AAGAAAGTGC CCATTAAGAT AGTCCATTCA 
GAGAGTGACA CGGAAAAAGA AAGTCGGCAA TATCTAGACC TGCCCAGCGA GACGCCTGTC 
AACTCGCAGG GACTTGTGGG AGCTCATCTT CAAAGTTTGG GGAACCCGGA TCAGTCATAC 
TCTTTGTTTT GTACATACAC CAGACAAAAA GATCAGGTTC CTGGTCTTAG AGACCCAGAC 
ATGGGCCCTC TAAAAGACCA GGGGCCACAA AGCAATATAA ACCCAGTGTC TTATGTGGAA 
CCTGGCCTTC AGACAGGTCC ACCATTGGAT CAGAGTACCA ATGGAGTGTC TTTGCATCCT 
TCACATTCAG AGGATGATAA AAGAAAGGAG CTGGCTAGAG ACATAATGGA CAAAGACAAG 
TCCCTCGCTG ACATTTTAGA CCAGAGTAAG ATGAAGACCA CTATGGATCT GATGGAGGGG 
ATTTTCCCTC AAGGAGATCA GCTACTGGAG GAGGCCCAAC AGCGTAGGAA GGCTCAACCT 
AAACCACTCT CCCCTCGAAA CTCTGTTGAG AAGAAAGAGG AAGACAGCCT GGTGGCGGCC 
GCTGCTTTAG TGAGCAACTC AACCTACTAC AGCACATCTG CACCCAAAGC AGAGCTGCTG 
ATCAAGATGA AGGACATGCA GGACCAGAGC GTTGAACACA ACTCAGAAGA GGAGCTGGAG 
GAGGACAATG AAAGTGACCT CGCCTGCAAG AAGCACGAGT TGATTGAAAG TCTCAGTAAG 
AAGTTGCAGG TCTTAAAAGA AGCTCAGGAG AGCCTGCAGG AGGACGTGCA GGACAACAAC 
GCTCTTGGAG AAGAAGTGGA GGCCATCGTA CAGGGCGTCT GCAAGCCTAA TGAACTCGAC 
AAATTCCGCA TGTTCGTCGG GGATCTTGAT AAAGTGGTCA ACCTTCTGCT GTCTCTGTCA 
GGACGTTTGG CCAGGGTAGA GAATGCCCTG AACAGCCTGG AGGAAGATGC TTCGCTGGAG 
GAAAGGCGCA CTCTGACAGA GAAACGCAAA CTGCTGATTC GTCAGCACGA GGATGCTAAG 
GAGCTGAAGG AGAACCTGGA CAGGAGAGAG CGTGTGGTCT ACGACATTCT GGCCAGTTAC 
CTGCCTGAGG AGAGTATGGC TGACTACGAG CACTTTGTCA AGATGAAATC CGCTCTTATT 
ATCGAGCAAC GCAAGCTGGA GGACAAGATC AAACTGGGAG ATGAGCAGCT CAAGTGTCTG 
ATGGACAGTC TCCCAATGGA CCAGAGAATA ATCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2a ( XM_017356639.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212128.1 Danio rerio shroom family member 2a (shroom2a), transcript variant X8, mRNA.

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CloneID	ODa59477
Clone ID Related Accession (Same CDS sequence)	XM_017356639.1 , XM_017356639.2
Accession Version	XM_017356639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGAC CTCACTCCTG GCATGCCACC AAGTTCAACG AGACCCATTC AGAAGCCAAA 
ACCCAGTCCA CACCCTCACC AGTTTGGCAA ACAAGATATG ATGCAAGTTC ATTCTCTTCT 
GAACTCTCAA CTGGCTGGGA TCAAACAAAT CTACGGAGAG TATCCGATCA GTTCAGTTCT 
TTGGGGAGTA TGGACAGCCT AGAACATAGT TCTCATCCCT ATCCACCTGG CCGTCTTTCC 
CCAAACAAAT CCAACAATAA TAGCATTGAA CATTTGGGTA GTGGTAAACG GGACTCGGCA 
TACAGCTCTT TTTCTACAAG TTCGGGAACC CCAGATTACA CACTGTCCAA AAGTAACACG 
GCATCCACTG AGAACATGCT GTACAAAATA AATCAGTGGG ATTCAAGTGC CAGGCACAGC 
AATGGCAGGC ACAGCCAAAA CCTAAGCGAA GGGGTACGAC AAGATGAGAG GCCTGGATAC 
CTGCACCACC TCTCAAGTAC AGGTAACCAC GAGATCCCAA GAGCTGAAGA ACTGCCTGGC 
AACCGTCATT CAAGCTCTGG AAGAGTTAAT ATTGGACCTG TCTGGCATGT TCCAGATATG 
AAGAAAAACA TGATATCATC TACTCCACCT CCCACTCCAC CCACACGCAG TGATAGCTTT 
GCAGCTACCA AGGTTCACGA GAAGGGTCTG ATCACAGGTA CTTCAGAGGG CCTTGGTGGT 
CATTCTCAAG TAAAGCCTCA AGCGAAACCA CAACAAAAGC CAGGAGAAAC CCATGAAAGC 
ACACAAAGGT CTAACCAAGT CATCGAGATT TGTCTTGAGG GTCGACGGGA TTATAATCTA 
CAATCCAAAA ATGATTCCTC AAATAGCTGC ATTTCATCTG ATTCTCATCA TCAGTATATC 
CAACGTACAT CATCAGATAA GACATACTCC CTATCAACCA CAGACATAAG AGATAGCCAT 
CCATCCTATG GCCATGCTCC ATACCATGCA CAGCAGTACA GTGACGAAGG CACTTTCCAT 
GCTCAAACTA GAACAGCAAT GAAACCTCCA TTCAGTGGCT ATTTCAGTAG TATGCAGGAA 
CTGCACACAA ACAACCACAT GCAACTCAAC AGCCAGAATC AAACTGGAAG ACCAGCTGCA 
TCACTGTCAG TTAATTTAGG AGTCAATTCC CACCATATCT CTCAGGGATT AACACAGGCT 
TCTTTAATGA GAGCTGATGA CTCTAAAGCC TCTTCAGTCT CAGAAATGTC CCACAATGGA 
CGGGACAGAA TGTCCATAGG TTCTCAAGGA GGGAATAAAG AGCGGTATTT CCCTCCTCAA 
TCGCAGCACC ATGATAATAA AGAAAGAAGT GCCTCCTTTA AACAAAATGA AAACCAACAT 
TATGTTTCTG CACCAAGTAA TCCCTTGAAT ATGTCTGATT CAACAAAACA TAATGAGCTT 
AGGGGATGCC AAAAGCAGCA CCATGTGCAT GGCTCAGATG AGCTTTCTGG TAGTTATCCA 
CCCAGTAAGC AGCCCGAGCA CAGGAGAAGT GCTGGCCATC TTCATCATAA GGAATACTCG 
CAACAACCCT TTCCAACTAA AAGCGAATCA AAGATATGTC CCCAGAAAAC ACCTATGTTG 
TATTCTCTGG CCCAAGGGCA CAATGACACA GATGACTGTT CTAATCAGAT CAAAAGTGGA 
AGCGCACAAC ATGAAGCCCT TGACAGTCTC AGTAGTAAAC AGGTTAGACG GAATGACCGA 
TTTGCCACCA CTTTGCGCAA CGAGATCCAG ATGAGAAGGG CGCATCTCCA AAAGAGTCAA 
AGTGCAGCCA CTTTAGAAAG TCCAGTTGAA ACTGTAGAAG AGTCTGCTTT GTGGAAGACC 
ACAGGGACTG CTTCTCCCTC ATCAGATGGC TGCTTTTCCA TTTCTTATAA AGACCATCTT 
AAAGAAGCTC AGGCCAGAGT CCTCCAGGCT ACATCATTCA GGAGAAAAGA CCTAGAGCCA 
GTATTATTTG AGCATCCAGG TACTGAAGGT CCCACACGAA AAGATGTACC TCCTTCAAGT 
GTCTCAGAGG TCACACCTAG CAAACCCACC TCAGGGAGTA ATCATGTGCT TCGCATTGGA 
GGCCGTAAGC GGATATCTGC TGAAAAGAAA GTGACGTCTT TTTCTGAGCC AGATAAAATT 
CATGAAGTAG GAGCTGATGA ACGCTCCACT ATGCCTGAAA ATGTTGCACA CTTAGAAAAT 
CAACAAAGAT TCTTTGAGAC AACAGGGAAA CCAGTTTTCC CTAAGCCTAT GCCAAAGCAA 
AACCTGCAGA TATCAGAAGA CCCCAGACTG TCCAAGCCTG GAGGCATGCA CTATTCTGCA 
AACAGTGATA TGGCAGGGCG GAATAATGGT GAAAGCTCAA CCCCCATTGA ACACCACATT 
AATGAAGGTC TGGATGGCCC TAACTCAGTG AACCATCAAG CATTGCTAGA ACAACAGCGA 
CTAGGCACAT TTGCAGAGTA TGAGGCCAAA TGGAACACCC AGAGGAAGAC TACAGAACCA 
AGAGTATCTG GCCGATACCA CTCTGCTGAT AACATCCTCG ATACAGGAAA AGAGCGACGG 
AGTAACCCCA CCTGTGTGCA TGAAAGATCT AGATCCTCAC CTTCTGCTGA CTTTTATGGG 
CAGAAACTTC CACCACAAGA AAAGAAGTCA GCTGACTGTT TCAAATCTGA AAAGAGTCCT 
TGCCAACAGG ACAAGAATAG TACAAGTCAA AATTTCTACC CATCTGTTTC TTTGGAGCAC 
AGCCACTGGT TCTATACACC CAGCAGGTTA AATGAAAAAG GATATAGTGA AATCTTATGC 
AAGGAGAAAC CAGAAACACC TCCTGAAGAA CCTGAGAAAA AGACTGTAGA TCCTCCCTCC 
AGCCATCATA AGCCTGCACT ACATTTCCCT GTCCATAGCA CTCGCAGTGA ACCATCAGCC 
CTCTCCAAAA ACAAGAGCTC ATTTCTCTTG CCCCATCTGG AGAAGTACAA ATGCCCTGAG 
GGAACACCTC CTCCTCTTAG CAATATTCAG GAGGTCCAGC CTGGATCACA AGGAGGAGCT 
CTGGCCCCAA TTTCCCCAGT CAACAATCAA AGCTCCGCCC CAGTTTCAGC CCCCATTCCT 
TGGAAGAGTC CAGAGTACAG CAAAGGGCCT GTCGGGGAGG AGGAGCAACA CCAAGAGCTT 
GTGCCTCCTC CCTTTCCACC TCCTCCCCCT CCCGTTGTCC TGCCCACCCA ACAAACCGCT 
GGCCCGACCC AGCCCACAAT GGATGGGCAG CGTTCGCCCT CGCCCCAGTT CGCTCCCCAG 
AGGCTGACTG ACAAGCCCCC TGTCTCCGTC TCCTCACAGG ATGAAGCTCC TGGAAGGATG 
GATCAGATTA AAGATGAGAA CACATCTGTG AAGAAAGTGC CCATTAAGAT AGTCCATTCA 
GAGAGTGACA CGGAAAAAGA AAGTCGGCAA TATCTAGACC TGCCCAGCGA GACGCCTGTC 
AACTCGCAGG GACTTGTGGG AGCTCATCTT CAAAGTTTGG GGAACCCGGA TCAGTCATAC 
TCTTTGTTTT GTACATACAC CAGACAAAAA GATCAGGTTC CTGGTCTTAG AGACCCAGAC 
ATGGGCCCTC TAAAAGACCA GGGGCCACAA AGCAATATAA ACCCAGTGTC TTATGTGGAA 
CCTGGCCTTC AGACAGGTCC ACCATTGGAT CAGAGTACCA ATGGAGTGTC TTTGCATCCT 
TCACATTCAG AGGATGATAA AAGAAAGGAG CTGGCTAGAG ACATAATGGA CAAAGACAAG 
TCCCTCGCTG ACATTTTAGA CCAGAGTAAG ATGAAGACCA CTATGGATCT GATGGAGGGG 
ATTTTCCCTC AAGGAGATCA GCTACTGGAG GAGGCCCAAC AGCGTAGGAA GGCTCAACCT 
AAACCACTCT CCCCTCGAAA CTCTGTTGAG AAGAAAGAGG AAGACAGCCT GGTGGCGGCC 
GCTGCTTTAG TGAGCAACTC AACCTACTAC AGCACATCTG CACCCAAAGC AGAGCTGCTG 
ATCAAGATGA AGGACATGCA GGACCAGAGC GTTGAACACA ACTCAGAAGA GGAGCTGGAG 
GAGGACAATG AAAGTGACCT CGCCTGCAAG AAGCACGAGT TGATTGAAAG TCTCAGTAAG 
AAGTTGCAGG TCTTAAAAGA AGCTCAGGAG AGCCTGCAGG AGGACGTGCA GGACAACAAC 
GCTCTTGGAG AAGAAGTGGA GGCCATCGTA CAGGGCGTCT GCAAGCCTAA TGAACTCGAC 
AAATTCCGCA TGTTCGTCGG GGATCTTGAT AAAGTGGTCA ACCTTCTGCT GTCTCTGTCA 
GGACGTTTGG CCAGGGTAGA GAATGCCCTG AACAGCCTGG AGGAAGATGC TTCGCTGGAG 
GAAAGGCGCA CTCTGACAGA GAAACGCAAA CTGCTGATTC GTCAGCACGA GGATGCTAAG 
GAGCTGAAGG AGAACCTGGA CAGGAGAGAG CGTGTGGTCT ACGACATTCT GGCCAGTTAC 
CTGCCTGAGG AGAGTATGGC TGACTACGAG CACTTTGTCA AGATGAAATC CGCTCTTATT 
ATCGAGCAAC GCAAGCTGGA GGACAAGATC AAACTGGGAG ATGAGCAGCT CAAGTGTCTG 
ATGGACAGTC TCCCAATGGA CCAGAGAATA ATCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212128.1
CDS	633..5291
Translation	MSRPHSWHATKFNETHSEAKTQSTPSPVWQTRYDASSFSSELSTGWDQTNLRRVSDQFSSLGSMDSLEHSSHPYPPGRLSPNKSNNNSIEHLGSGKRDSAYSSFSTSSGTPDYTLSKSNTASTENMLYKINQWDSSARHSNGRHSQNLSEGVRQDERPGYLHHLSSTGNHEIPRAEELPGNRHSSSGRVNIGPVWHVPDMKKNMISSTPPPTPPTRSDSFAATKVHEKGLITGTSEGLGGHSQVKPQAKPQQKPGETHESTQRSNQVIEICLEGRRDYNLQSKNDSSNSCISSDSHHQYIQRTSSDKTYSLSTTDIRDSHPSYGHAPYHAQQYSDEGTFHAQTRTAMKPPFSGYFSSMQELHTNNHMQLNSQNQTGRPAASLSVNLGVNSHHISQGLTQASLMRADDSKASSVSEMSHNGRDRMSIGSQGGNKERYFPPQSQHHDNKERSASFKQNENQHYVSAPSNPLNMSDSTKHNELRGCQKQHHVHGSDELSGSYPPSKQPEHRRSAGHLHHKEYSQQPFPTKSESKICPQKTPMLYSLAQGHNDTDDCSNQIKSGSAQHEALDSLSSKQVRRNDRFATTLRNEIQMRRAHLQKSQSAATLESPVETVEESALWKTTGTASPSSDGCFSISYKDHLKEAQARVLQATSFRRKDLEPVLFEHPGTEGPTRKDVPPSSVSEVTPSKPTSGSNHVLRIGGRKRISAEKKVTSFSEPDKIHEVGADERSTMPENVAHLENQQRFFETTGKPVFPKPMPKQNLQISEDPRLSKPGGMHYSANSDMAGRNNGESSTPIEHHINEGLDGPNSVNHQALLEQQRLGTFAEYEAKWNTQRKTTEPRVSGRYHSADNILDTGKERRSNPTCVHERSRSSPSADFYGQKLPPQEKKSADCFKSEKSPCQQDKNSTSQNFYPSVSLEHSHWFYTPSRLNEKGYSEILCKEKPETPPEEPEKKTVDPPSSHHKPALHFPVHSTRSEPSALSKNKSSFLLPHLEKYKCPEGTPPPLSNIQEVQPGSQGGALAPISPVNNQSSAPVSAPIPWKSPEYSKGPVGEEEQHQELVPPPFPPPPPPVVLPTQQTAGPTQPTMDGQRSPSPQFAPQRLTDKPPVSVSSQDEAPGRMDQIKDENTSVKKVPIKIVHSESDTEKESRQYLDLPSETPVNSQGLVGAHLQSLGNPDQSYSLFCTYTRQKDQVPGLRDPDMGPLKDQGPQSNINPVSYVEPGLQTGPPLDQSTNGVSLHPSHSEDDKRKELARDIMDKDKSLADILDQSKMKTTMDLMEGIFPQGDQLLEEAQQRRKAQPKPLSPRNSVEKKEEDSLVAAAALVSNSTYYSTSAPKAELLIKMKDMQDQSVEHNSEEELEEDNESDLACKKHELIESLSKKLQVLKEAQESLQEDVQDNNALGEEVEAIVQGVCKPNELDKFRMFVGDLDKVVNLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASYLPEESMADYEHFVKMKSALIIEQRKLEDKIKLGDEQLKCLMDSLPMDQRIIN

Target ORF information:

RefSeq Version	XM_017356639.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 2a (shroom2a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356639.2

ORF Insert Sequence:

ATGAGCAGAC CTCACTCCTG GCATGCCACC AAGTTCAACG AGACCCATTC AGAAGCCAAA 
ACCCAGTCCA CACCCTCACC AGTTTGGCAA ACAAGATATG ATGCAAGTTC ATTCTCTTCT 
GAACTCTCAA CTGGCTGGGA TCAAACAAAT CTACGGAGAG TATCCGATCA GTTCAGTTCT 
TTGGGGAGTA TGGACAGCCT AGAACATAGT TCTCATCCCT ATCCACCTGG CCGTCTTTCC 
CCAAACAAAT CCAACAATAA TAGCATTGAA CATTTGGGTA GTGGTAAACG GGACTCGGCA 
TACAGCTCTT TTTCTACAAG TTCGGGAACC CCAGATTACA CACTGTCCAA AAGTAACACG 
GCATCCACTG AGAACATGCT GTACAAAATA AATCAGTGGG ATTCAAGTGC CAGGCACAGC 
AATGGCAGGC ACAGCCAAAA CCTAAGCGAA GGGGTACGAC AAGATGAGAG GCCTGGATAC 
CTGCACCACC TCTCAAGTAC AGGTAACCAC GAGATCCCAA GAGCTGAAGA ACTGCCTGGC 
AACCGTCATT CAAGCTCTGG AAGAGTTAAT ATTGGACCTG TCTGGCATGT TCCAGATATG 
AAGAAAAACA TGATATCATC TACTCCACCT CCCACTCCAC CCACACGCAG TGATAGCTTT 
GCAGCTACCA AGGTTCACGA GAAGGGTCTG ATCACAGGTA CTTCAGAGGG CCTTGGTGGT 
CATTCTCAAG TAAAGCCTCA AGCGAAACCA CAACAAAAGC CAGGAGAAAC CCATGAAAGC 
ACACAAAGGT CTAACCAAGT CATCGAGATT TGTCTTGAGG GTCGACGGGA TTATAATCTA 
CAATCCAAAA ATGATTCCTC AAATAGCTGC ATTTCATCTG ATTCTCATCA TCAGTATATC 
CAACGTACAT CATCAGATAA GACATACTCC CTATCAACCA CAGACATAAG AGATAGCCAT 
CCATCCTATG GCCATGCTCC ATACCATGCA CAGCAGTACA GTGACGAAGG CACTTTCCAT 
GCTCAAACTA GAACAGCAAT GAAACCTCCA TTCAGTGGCT ATTTCAGTAG TATGCAGGAA 
CTGCACACAA ACAACCACAT GCAACTCAAC AGCCAGAATC AAACTGGAAG ACCAGCTGCA 
TCACTGTCAG TTAATTTAGG AGTCAATTCC CACCATATCT CTCAGGGATT AACACAGGCT 
TCTTTAATGA GAGCTGATGA CTCTAAAGCC TCTTCAGTCT CAGAAATGTC CCACAATGGA 
CGGGACAGAA TGTCCATAGG TTCTCAAGGA GGGAATAAAG AGCGGTATTT CCCTCCTCAA 
TCGCAGCACC ATGATAATAA AGAAAGAAGT GCCTCCTTTA AACAAAATGA AAACCAACAT 
TATGTTTCTG CACCAAGTAA TCCCTTGAAT ATGTCTGATT CAACAAAACA TAATGAGCTT 
AGGGGATGCC AAAAGCAGCA CCATGTGCAT GGCTCAGATG AGCTTTCTGG TAGTTATCCA 
CCCAGTAAGC AGCCCGAGCA CAGGAGAAGT GCTGGCCATC TTCATCATAA GGAATACTCG 
CAACAACCCT TTCCAACTAA AAGCGAATCA AAGATATGTC CCCAGAAAAC ACCTATGTTG 
TATTCTCTGG CCCAAGGGCA CAATGACACA GATGACTGTT CTAATCAGAT CAAAAGTGGA 
AGCGCACAAC ATGAAGCCCT TGACAGTCTC AGTAGTAAAC AGGTTAGACG GAATGACCGA 
TTTGCCACCA CTTTGCGCAA CGAGATCCAG ATGAGAAGGG CGCATCTCCA AAAGAGTCAA 
AGTGCAGCCA CTTTAGAAAG TCCAGTTGAA ACTGTAGAAG AGTCTGCTTT GTGGAAGACC 
ACAGGGACTG CTTCTCCCTC ATCAGATGGC TGCTTTTCCA TTTCTTATAA AGACCATCTT 
AAAGAAGCTC AGGCCAGAGT CCTCCAGGCT ACATCATTCA GGAGAAAAGA CCTAGAGCCA 
GTATTATTTG AGCATCCAGG TACTGAAGGT CCCACACGAA AAGATGTACC TCCTTCAAGT 
GTCTCAGAGG TCACACCTAG CAAACCCACC TCAGGGAGTA ATCATGTGCT TCGCATTGGA 
GGCCGTAAGC GGATATCTGC TGAAAAGAAA GTGACGTCTT TTTCTGAGCC AGATAAAATT 
CATGAAGTAG GAGCTGATGA ACGCTCCACT ATGCCTGAAA ATGTTGCACA CTTAGAAAAT 
CAACAAAGAT TCTTTGAGAC AACAGGGAAA CCAGTTTTCC CTAAGCCTAT GCCAAAGCAA 
AACCTGCAGA TATCAGAAGA CCCCAGACTG TCCAAGCCTG GAGGCATGCA CTATTCTGCA 
AACAGTGATA TGGCAGGGCG GAATAATGGT GAAAGCTCAA CCCCCATTGA ACACCACATT 
AATGAAGGTC TGGATGGCCC TAACTCAGTG AACCATCAAG CATTGCTAGA ACAACAGCGA 
CTAGGCACAT TTGCAGAGTA TGAGGCCAAA TGGAACACCC AGAGGAAGAC TACAGAACCA 
AGAGTATCTG GCCGATACCA CTCTGCTGAT AACATCCTCG ATACAGGAAA AGAGCGACGG 
AGTAACCCCA CCTGTGTGCA TGAAAGATCT AGATCCTCAC CTTCTGCTGA CTTTTATGGG 
CAGAAACTTC CACCACAAGA AAAGAAGTCA GCTGACTGTT TCAAATCTGA AAAGAGTCCT 
TGCCAACAGG ACAAGAATAG TACAAGTCAA AATTTCTACC CATCTGTTTC TTTGGAGCAC 
AGCCACTGGT TCTATACACC CAGCAGGTTA AATGAAAAAG GATATAGTGA AATCTTATGC 
AAGGAGAAAC CAGAAACACC TCCTGAAGAA CCTGAGAAAA AGACTGTAGA TCCTCCCTCC 
AGCCATCATA AGCCTGCACT ACATTTCCCT GTCCATAGCA CTCGCAGTGA ACCATCAGCC 
CTCTCCAAAA ACAAGAGCTC ATTTCTCTTG CCCCATCTGG AGAAGTACAA ATGCCCTGAG 
GGAACACCTC CTCCTCTTAG CAATATTCAG GAGGTCCAGC CTGGATCACA AGGAGGAGCT 
CTGGCCCCAA TTTCCCCAGT CAACAATCAA AGCTCCGCCC CAGTTTCAGC CCCCATTCCT 
TGGAAGAGTC CAGAGTACAG CAAAGGGCCT GTCGGGGAGG AGGAGCAACA CCAAGAGCTT 
GTGCCTCCTC CCTTTCCACC TCCTCCCCCT CCCGTTGTCC TGCCCACCCA ACAAACCGCT 
GGCCCGACCC AGCCCACAAT GGATGGGCAG CGTTCGCCCT CGCCCCAGTT CGCTCCCCAG 
AGGCTGACTG ACAAGCCCCC TGTCTCCGTC TCCTCACAGG ATGAAGCTCC TGGAAGGATG 
GATCAGATTA AAGATGAGAA CACATCTGTG AAGAAAGTGC CCATTAAGAT AGTCCATTCA 
GAGAGTGACA CGGAAAAAGA AAGTCGGCAA TATCTAGACC TGCCCAGCGA GACGCCTGTC 
AACTCGCAGG GACTTGTGGG AGCTCATCTT CAAAGTTTGG GGAACCCGGA TCAGTCATAC 
TCTTTGTTTT GTACATACAC CAGACAAAAA GATCAGGTTC CTGGTCTTAG AGACCCAGAC 
ATGGGCCCTC TAAAAGACCA GGGGCCACAA AGCAATATAA ACCCAGTGTC TTATGTGGAA 
CCTGGCCTTC AGACAGGTCC ACCATTGGAT CAGAGTACCA ATGGAGTGTC TTTGCATCCT 
TCACATTCAG AGGATGATAA AAGAAAGGAG CTGGCTAGAG ACATAATGGA CAAAGACAAG 
TCCCTCGCTG ACATTTTAGA CCAGAGTAAG ATGAAGACCA CTATGGATCT GATGGAGGGG 
ATTTTCCCTC AAGGAGATCA GCTACTGGAG GAGGCCCAAC AGCGTAGGAA GGCTCAACCT 
AAACCACTCT CCCCTCGAAA CTCTGTTGAG AAGAAAGAGG AAGACAGCCT GGTGGCGGCC 
GCTGCTTTAG TGAGCAACTC AACCTACTAC AGCACATCTG CACCCAAAGC AGAGCTGCTG 
ATCAAGATGA AGGACATGCA GGACCAGAGC GTTGAACACA ACTCAGAAGA GGAGCTGGAG 
GAGGACAATG AAAGTGACCT CGCCTGCAAG AAGCACGAGT TGATTGAAAG TCTCAGTAAG 
AAGTTGCAGG TCTTAAAAGA AGCTCAGGAG AGCCTGCAGG AGGACGTGCA GGACAACAAC 
GCTCTTGGAG AAGAAGTGGA GGCCATCGTA CAGGGCGTCT GCAAGCCTAA TGAACTCGAC 
AAATTCCGCA TGTTCGTCGG GGATCTTGAT AAAGTGGTCA ACCTTCTGCT GTCTCTGTCA 
GGACGTTTGG CCAGGGTAGA GAATGCCCTG AACAGCCTGG AGGAAGATGC TTCGCTGGAG 
GAAAGGCGCA CTCTGACAGA GAAACGCAAA CTGCTGATTC GTCAGCACGA GGATGCTAAG 
GAGCTGAAGG AGAACCTGGA CAGGAGAGAG CGTGTGGTCT ACGACATTCT GGCCAGTTAC 
CTGCCTGAGG AGAGTATGGC TGACTACGAG CACTTTGTCA AGATGAAATC CGCTCTTATT 
ATCGAGCAAC GCAAGCTGGA GGACAAGATC AAACTGGGAG ATGAGCAGCT CAAGTGTCTG 
ATGGACAGTC TCCCAATGGA CCAGAGAATA ATCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	shroom2a
Entrez Gene ID	386817
Full Name	shroom family member 2a
Synonyms	im:7141132,wu:fj08g11,wu:fl10b04
General protein information	Preferred Names shroom family member 2a Names protein Shroom2 shroom family member 2a fj08g11
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

shroom2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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