dnaaf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dnaaf1
Entrez Gene ID	386722
Full Name	dynein, axonemal, assembly factor 1
Synonyms	lrrc50,swt,zgc:56169
General protein information	Preferred Names dynein, axonemal, assembly factor 1 Names dynein assembly factor 1, axonemal leucine rich repeat containing 50 leucine-rich repeat-containing protein 50 switch hitter unm tm317b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001319131.1	NP_001306060.1	dynein assembly factor 1, axonemal

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

DNAAF1 links heart laterality with the AAA+ ATPase RUVBL1 and ciliary intraflagellar transport.
Hartill VL, van de Hoek G, Patel MP, Little R, Watson CM, Berry IR, Shoemark A, Abdelmottaleb D, Parkes E, Bacchelli C, Szymanska K, Knoers NV, Scambler PJ, Ueffing M, Boldt K, Yates R, Winyard PJ, Adler B, Moya E, Hattingh L, Shenoy A, Hogg C, Sheridan E, Roepman R, Norris D, Mitchison HM, Giles RH, Johnson CA
Human molecular genetics(2017 Dec)

Rare disruptive mutations in ciliary function genes contribute to testicular cancer susceptibility.
Litchfield K, Levy M, Dudakia D, Proszek P, Shipley C, Basten S, Rapley E, Bishop DT, Reid A, Huddart R, Broderick P, Castro DG, O'Connor S, Giles RH, Houlston RS, Turnbull C
Nature communications713840(2016 Dec)

Mutations in the Motile Cilia Gene DNAAF1 Are Associated with Neural Tube Defects in Humans.
Miao C, Jiang Q, Li H, Zhang Q, Bai B, Bao Y, Zhang T
G3 (Bethesda, Md.)6(10)3307-3316(2016 10)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

c21orf59/kurly Controls Both Cilia Motility and Polarization.
Jaffe KM, Grimes DT, Schottenfeld-Roames J, Werner ME, Ku TS, Kim SK, Pelliccia JL, Morante NF, Mitchell BJ, Burdine RD
Cell reports14(8)1841-9(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dnaaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dnaaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa13064	NM_001319131.1 Latest version!	Danio rerio dynein axonemal assembly factor 1 (dnaaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dnaaf1 ( NM_001319131.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001306060.1 Danio rerio dynein axonemal assembly factor 1 (dnaaf1), mRNA.

Price & Availability↑

CloneID	ODa13064
Clone ID Related Accession (Same CDS sequence)	NM_001319131.1
Accession Version	NM_001319131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-05
Organism	Danio rerio(zebrafish)
Product	dynein assembly factor 1, axonemal
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR376738.9. On Jan 26, 2016 this sequence version replaced XM_005168864.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01008226.1, GDQH01021787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAAGACAA AAATGATCGT ATCCACATCG ATGAACGCGG GAGAAGGAAC AAGAACTGAG 
GCTGAAGACA CTAGTAAAGG TGTTGCGTCT TTAGCAGATC TGGATTCTCA GAGTGCAGCT 
GCTGAAGTGT CAGAGAGTGA CATAAAGCCT TCAGGCCCCA GAATTACCAA AGACTTCTTA 
AGGGAGCACT GCAAGAAAAA CAAACTGTAC CTGACACCAA GGCTGAATGA CACACTTTAT 
CTTCACTTTA AAGGTTTTTC TTGTATAGAG GGTTTGGAAG AATATACTGG CCTGCGCTGC 
CTATGGCTCG AGTGTAATGG TATTCGAAAG ATTGAAAATC TGGAGAATCA GACAGAGCTT 
CGCTGTCTGT TCCTCCATCA GAATCTCATT CACACTCTGG AAAACCTGGA ACCACTCAGC 
AAGCTTTGCA CGCTTAATGT CTCAAACAAT TATATCAAGG TCATTGAGAA CATTTCTAGT 
TTGAGCGACT TGTCCACTCT ACAGATCTCT CATAACACAC TGGAGAATGT TTGTGACATG 
GAGGAGCTTT CACACTGTCC ATCCATCAGT GTCCTTGATC TGTCCCACAA CCGCATCAGT 
GATCCAGCGC TAGTTAATAT TCTAGAGAAA ATGCCTGACT TGCGGGTGTT AAATCTAATG 
GGCAATGAAG TCATCAAAAA AATCCCAAAC TACAGGAAGA CTCTGATTGT GCGTCTGAAG 
CAGCTGACCT ATCTAGATGA TCGGCCTGTT TTTCCAAAAG ATAGAGCATG TGCCGAGGCC 
TGGGCAGCTG GTGGTTTGGA AGGTGAGCAA AAAGAAAGGG AAATGTGGCA AACAAGAGAG 
AGAAGGAAGA TCCAGGAAAG TTTGGATGCA ATGACTGCCA TTCGAGAGAC TGCTCTGAAA 
CGGCGGCAGG ACCGAGAGCT TTCAGAGAAA GGAATTCTAG AGTTTCCAAG TCCAGTTGCT 
GAGGACCAGA GCCCACATGA TGAAATATCA GATATTGAAC CTGCAGAGAG TGACTCAGAT 
AGTGAAACAA CACAAGAACA AACTGATGCA TCTCCATCGC CATCAGAGAT GGACCACAAT 
TTCAACTCTG CATCTGATGA AGATCAGATT ATTCAAAAAA CACAAAATGA TTCAGACCAG 
TCACAACCAC AACCGAATGA AAGTGTTCAT TCATCATTTG ACGAGGAACA GATTCGCCAA 
TCAGCAACAT GGGGACTGAA GCCTAAAGAG GAGAAGAATC AAATTTTGAG CATCTACACA 
AAACAATCAC CGTCTGATCA GAGAGTTTTA ATCTCAGTCT CAGGTCCCGG TGCTCTTGTC 
ACTGAACTGA TCTCTGAAGA CGAAATAGAG TCTATCAACC TTTCAGATGA ACCTGTATTA 
ACAATTGATG ATCTTCCAGA TCTTGAGGAT GAAGAAAATA GCCACATACT ACCAATAACA 
TCACAGAGTG GGATGCGTCC AAAGATTGAG GTGATCTCCA GCATTGACAC AGAGTCAGAA 
GCTGTTAGGA GTTTGTATCC CTGGCCTCAT TCAGACGCTG TGGCCCTTGA CCAATCTCAG 
CAGCTTATCA GTGTCTCCAC TACAAGAAAC TCAGTCAAAC AGTCTGGCAT CAGTCTGGCA 
GAGGCAGGAG ATGACGTCCC AAAGGCAGCA GAAAAAAGAA AGACTGTTCT GATTGAGGAA 
CTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001306060.1
CDS	108..1796
Translation	MKTKMIVSTSMNAGEGTRTEAEDTSKGVASLADLDSQSAAAEVSESDIKPSGPRITKDFLREHCKKNKLYLTPRLNDTLYLHFKGFSCIEGLEEYTGLRCLWLECNGIRKIENLENQTELRCLFLHQNLIHTLENLEPLSKLCTLNVSNNYIKVIENISSLSDLSTLQISHNTLENVCDMEELSHCPSISVLDLSHNRISDPALVNILEKMPDLRVLNLMGNEVIKKIPNYRKTLIVRLKQLTYLDDRPVFPKDRACAEAWAAGGLEGEQKEREMWQTRERRKIQESLDAMTAIRETALKRRQDRELSEKGILEFPSPVAEDQSPHDEISDIEPAESDSDSETTQEQTDASPSPSEMDHNFNSASDEDQIIQKTQNDSDQSQPQPNESVHSSFDEEQIRQSATWGLKPKEEKNQILSIYTKQSPSDQRVLISVSGPGALVTELISEDEIESINLSDEPVLTIDDLPDLEDEENSHILPITSQSGMRPKIEVISSIDTESEAVRSLYPWPHSDAVALDQSQQLISVSTTRNSVKQSGISLAEAGDDVPKAAEKRKTVLIEELD

Target ORF information:

RefSeq Version	NM_001319131.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dynein axonemal assembly factor 1 (dnaaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001319131.1

ORF Insert Sequence:

ATGAAGACAA AAATGATCGT ATCCACATCG ATGAACGCGG GAGAAGGAAC AAGAACTGAG 
GCTGAAGACA CTAGTAAAGG TGTTGCGTCT TTAGCAGATC TGGATTCTCA GAGTGCAGCT 
GCTGAAGTGT CAGAGAGTGA CATAAAGCCT TCAGGCCCCA GAATTACCAA AGACTTCTTA 
AGGGAGCACT GCAAGAAAAA CAAACTGTAC CTGACACCAA GGCTGAATGA CACACTTTAT 
CTTCACTTTA AAGGTTTTTC TTGTATAGAG GGTTTGGAAG AATATACTGG CCTGCGCTGC 
CTATGGCTCG AGTGTAATGG TATTCGAAAG ATTGAAAATC TGGAGAATCA GACAGAGCTT 
CGCTGTCTGT TCCTCCATCA GAATCTCATT CACACTCTGG AAAACCTGGA ACCACTCAGC 
AAGCTTTGCA CGCTTAATGT CTCAAACAAT TATATCAAGG TCATTGAGAA CATTTCTAGT 
TTGAGCGACT TGTCCACTCT ACAGATCTCT CATAACACAC TGGAGAATGT TTGTGACATG 
GAGGAGCTTT CACACTGTCC ATCCATCAGT GTCCTTGATC TGTCCCACAA CCGCATCAGT 
GATCCAGCGC TAGTTAATAT TCTAGAGAAA ATGCCTGACT TGCGGGTGTT AAATCTAATG 
GGCAATGAAG TCATCAAAAA AATCCCAAAC TACAGGAAGA CTCTGATTGT GCGTCTGAAG 
CAGCTGACCT ATCTAGATGA TCGGCCTGTT TTTCCAAAAG ATAGAGCATG TGCCGAGGCC 
TGGGCAGCTG GTGGTTTGGA AGGTGAGCAA AAAGAAAGGG AAATGTGGCA AACAAGAGAG 
AGAAGGAAGA TCCAGGAAAG TTTGGATGCA ATGACTGCCA TTCGAGAGAC TGCTCTGAAA 
CGGCGGCAGG ACCGAGAGCT TTCAGAGAAA GGAATTCTAG AGTTTCCAAG TCCAGTTGCT 
GAGGACCAGA GCCCACATGA TGAAATATCA GATATTGAAC CTGCAGAGAG TGACTCAGAT 
AGTGAAACAA CACAAGAACA AACTGATGCA TCTCCATCGC CATCAGAGAT GGACCACAAT 
TTCAACTCTG CATCTGATGA AGATCAGATT ATTCAAAAAA CACAAAATGA TTCAGACCAG 
TCACAACCAC AACCGAATGA AAGTGTTCAT TCATCATTTG ACGAGGAACA GATTCGCCAA 
TCAGCAACAT GGGGACTGAA GCCTAAAGAG GAGAAGAATC AAATTTTGAG CATCTACACA 
AAACAATCAC CGTCTGATCA GAGAGTTTTA ATCTCAGTCT CAGGTCCCGG TGCTCTTGTC 
ACTGAACTGA TCTCTGAAGA CGAAATAGAG TCTATCAACC TTTCAGATGA ACCTGTATTA 
ACAATTGATG ATCTTCCAGA TCTTGAGGAT GAAGAAAATA GCCACATACT ACCAATAACA 
TCACAGAGTG GGATGCGTCC AAAGATTGAG GTGATCTCCA GCATTGACAC AGAGTCAGAA 
GCTGTTAGGA GTTTGTATCC CTGGCCTCAT TCAGACGCTG TGGCCCTTGA CCAATCTCAG 
CAGCTTATCA GTGTCTCCAC TACAAGAAAC TCAGTCAAAC AGTCTGGCAT CAGTCTGGCA 
GAGGCAGGAG ATGACGTCCC AAAGGCAGCA GAAAAAAGAA AGACTGTTCT GATTGAGGAA 
CTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNAAF1 links heart laterality with the AAA+ ATPase RUVBL1 and ciliary intraflagellar transport.
Human molecular genetics(2017 Dec)
Hartill VL,van de Hoek G,Patel MP,Little R,Watson CM,Berry IR,Shoemark A,Abdelmottaleb D,Parkes E,Bacchelli C,Szymanska K,Knoers NV,Scambler PJ,Ueffing M,Boldt K,Yates R,Winyard PJ,Adler B,Moya E,Hattingh L,Shenoy A,Hogg C,Sheridan E,Roepman R,Norris D,Mitchison HM,Giles RH,Johnson CA

Rare disruptive mutations in ciliary function genes contribute to testicular cancer susceptibility.
Nature communications713840(2016 Dec)
Litchfield K,Levy M,Dudakia D,Proszek P,Shipley C,Basten S,Rapley E,Bishop DT,Reid A,Huddart R,Broderick P,Castro DG,O'Connor S,Giles RH,Houlston RS,Turnbull C

Mutations in the Motile Cilia Gene DNAAF1 Are Associated with Neural Tube Defects in Humans.
G3 (Bethesda, Md.)6(10)3307-3316(2016 10)
Miao C,Jiang Q,Li H,Zhang Q,Bai B,Bao Y,Zhang T

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

c21orf59/kurly Controls Both Cilia Motility and Polarization.
Cell reports14(8)1841-9(2016 Mar)
Jaffe KM,Grimes DT,Schottenfeld-Roames J,Werner ME,Ku TS,Kim SK,Pelliccia JL,Morante NF,Mitchell BJ,Burdine RD

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

dnaaf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報