rgs12b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rgs12b
Entrez Gene ID	378970
Full Name	regulator of G protein signaling 12b
Synonyms	RGS12TS,RGS12TS-I,RGS12TS-L,RGS12TS-S,cb958,rgs12
General protein information	Preferred Names regulator of G protein signaling 12b Names regulator of G-protein signaling 12 regulator of G-protein signalling 12
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009293006.2	XP_009291281.1	regulator of G-protein signaling 12 isoform X5
XM_021481388.1	XP_021337063.1	regulator of G-protein signaling 12 isoform X1
XM_009292995.3	XP_009291270.1	regulator of G-protein signaling 12 isoform X3
XM_009293006.3	XP_009291281.1	regulator of G-protein signaling 12 isoform X4
NM_214724.1	NP_999889.1	regulator of G-protein signaling 12
XM_009292984.2	XP_009291259.1	regulator of G-protein signaling 12 isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC101731678	XP_004911316.1
Canis lupus familiaris (dog)	RGS12	XP_850554.1
Bos taurus (cattle)	RGS12	XP_005208281.1
Mus musculus (house mouse)	Rgs12	NP_775578.2
Homo sapiens (human)	RGS12	NP_937872.1
Rattus norvegicus (Norway rat)	Rgs12	NP_062212.1
Gallus gallus (chicken)	RGS12	XP_420820.3
Pan troglodytes (chimpanzee)	RGS12	XP_003310274.1
	RGS12	XP_002802183.1
Danio rerio (zebrafish)	rgs12b	NP_999889.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rgs12b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rgs12b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa40341	XM_009292995.3 Latest version!	Danio rerio regulator of G protein signaling 12b (rgs12b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa40342	XM_009293006.3 Latest version!	Danio rerio regulator of G protein signaling 12b (rgs12b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa40339	XM_009292984.2 Latest version!	Danio rerio regulator of G protein signaling 12b (rgs12b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa36013	NM_214724.1 Latest version!	Danio rerio regulator of G protein signaling 12b (rgs12b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa40338	XM_021481388.1 Latest version!	Danio rerio regulator of G protein signaling 12b (rgs12b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa40342	XM_009293006.2	Danio rerio regulator of G-protein signaling 12b (rgs12b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rgs12b ( XM_009292995.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291270.1 Danio rerio regulator of G protein signaling 12b (rgs12b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa40341
Clone ID Related Accession (Same CDS sequence)	XM_009292995.3
Accession Version	XM_009292995.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	regulator of G-protein signaling 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292995.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTTAC AGAGCGTGGA GTCATTTAAC GCTCGACTGT GGAGTCTAAC ACTGCCTTAC 
ATGGAGCCAA CTTTGAGCGT CAATCCTGAT GGATCTGCAG CTGTCTCTGA TGGTGAGCTT 
AACAGTGTGG ACCTGAAAGG CTGCGGCAGT GATAACAGTC TGAACAGCAA TGCCAGCCTA 
CCTAGCGTGC AATGCCATAG ACGGCACACT GAACGGCGCG TTGCATCGTG GGCCGTCTGC 
TTTGAGCGCC TGCTACAGGA TCCCGTCGGT GTCCGATATT TCTCGGAGTT TCTAAAGAAG 
GAGTTTAGCG AAGAGAATAT TTTATTTTGG CAAGCTTGTG AATATTTCAG CCATGTACCT 
GAAACAGACA AGAAACAGCT GTCCCAGCGG GCCAGAGAGA TCTACAACAG CTTCCTGTCT 
AGTAAAGCTA CCACACCCGT CAACATTGAT AGTCAAGCTC AGCTGGCTGA CGACATCCTC 
AACGCACCGA GGCCAGACAT GTTTAAAGAG CAACAGCTTC AGATCTTCAA CCTGATGAAG 
TTTGACAGTT ACACACGCTT CCTAAAGTCT TACCTGTATC AGGAATGTAT GCTTGCAGAG 
GTGGAGGGCC GTCCACTTCC AGACCCCTAC CAGGTGCCCA GTAGCCCCAC ATCCAAACAT 
AGCACAGGTT CCGACCGCTC CAACCTCTCT ACACCTAAAA AGGTGCAGGA CGACAAGAAG 
AGCAAATCGG GAAGGTCACT GAATGAAGAC AGCAGAGATG AACATGGCGA TAAAAAAAAA 
AGTATTTTCT TCTCTTGGTC CAGAAATAGA AGTTTTGGAA AAGGGTCGAA AAAAAGAGAC 
CTCGCAGATT ACAGCTTTAC AGGTAGTAAC GGAAGGAGAG AATCACAAGG CTCTTTGTCG 
TCTGGTGCGA GTTTGGAACT TGCCACTTCC GGTTCTGGAA AAAATGAGGG TGAGGTGTCG 
CGTAACTCCA TGTCGTTGTC TGAGCGTGCC GTGCGTCACT GTAACATTAA CCTACCGGAT 
GGCTCATGCT GCTCAGTACC CATCAGGCCC GGCGTCTCCA TCAGAGAGGT GCTCTTAGGC 
TTGTGTGAGA AACTAGGCAT TAATCTGGCT GCTGTGGACC TGTTCCTAGT AGGAGGAGAA 
AAGCCTTTAG TGTTGGACCA GGACTGCATG ACACTGTGCT CCAGGGATCT GCGGCTGGAG 
AAACGTACGC TATTCCGACT GGATCTGGTG CCTATAAATC GTTCTGTTGG ACTGAAAGCA 
AAACCCACTA AGCCGGTTAC AGAAGTGCTG AGACCAGTGG TGGCTAAATA TGGTTTAAAC 
TTAGGAGACC TGGTGGCCAC GATAAGTGGA GAGAAAGAGC CGCTGGATCT TGGGCTTCCT 
ATATCTAATC TAGATGGCCT GAGGGTGGTT CTTGAAGCAG GTGACCACGT TTCTGGGAAA 
GATAAACAAA AGCTGGTCTC ATCGAAAAGT CACGGTCCAC CTCTGTCTAC ACGAAGCCAA 
TCTGCCACAG GGGAGGACAA AACGGCAGGA AAGGCTTCGG CTGTGAAAAC CAGGGCCCAG 
GTGGAAAAGC GAAAACAGAA AAAGCTTAAT ATAGATGAAG CTGAAGAATT CTTCGAGCTC 
ATATCCAAAG CACAGAGCAA CAGAGCTGAC GACCAGCGGG GTTTGCTGAA CAAATCAGAC 
CTTGTCCTGC CCGACTTCCT GCGCCTCGAC ACGGAAGCAG ACGGCAACAA CCTCAGCTCC 
ACACCCATCT CCCACAAGAC TCGACCACGA TCCAAGGAGA ATGGCTCTGC CCTCAGTGCC 
AGTCACCAAA CGCAGAGCTT AGATTCAGCG GGGGAGGGCA AAGCACCCAG ACGAGCACTA 
ATGCCACCCA ACAGTGCCCT CAGAAACACA TCCAGCCGAA TTCCGCCACC GTTCAGTTCC 
TCGCTCTCCC CAATCCCACA TTCGCAAATG GGGGGCTCGT CCGGGATCAC AGACTGGAGG 
AGTGACGGAG GGGTCCAGGC ACTGGAGGAA GAGGAGAGCG GCATGGACCT GACTTTTGTT 
GCCGAAGGCG ACATCACAAG CCCTAACAGC TCTCTCCTTC ACCAGTCCCC CATTCCTCTC 
CCTCCTTCCC CAGAGCGATC ACACCTCCTG AAGGAGGACA CTTGCCAGGA CAGCTGGGCT 
CGATCAGGTA CCTCTCCTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291270.1
CDS	123..2306
Translation	MCLQSVESFNARLWSLTLPYMEPTLSVNPDGSAAVSDGELNSVDLKGCGSDNSLNSNASLPSVQCHRRHTERRVASWAVCFERLLQDPVGVRYFSEFLKKEFSEENILFWQACEYFSHVPETDKKQLSQRAREIYNSFLSSKATTPVNIDSQAQLADDILNAPRPDMFKEQQLQIFNLMKFDSYTRFLKSYLYQECMLAEVEGRPLPDPYQVPSSPTSKHSTGSDRSNLSTPKKVQDDKKSKSGRSLNEDSRDEHGDKKKSIFFSWSRNRSFGKGSKKRDLADYSFTGSNGRRESQGSLSSGASLELATSGSGKNEGEVSRNSMSLSERAVRHCNINLPDGSCCSVPIRPGVSIREVLLGLCEKLGINLAAVDLFLVGGEKPLVLDQDCMTLCSRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLNLGDLVATISGEKEPLDLGLPISNLDGLRVVLEAGDHVSGKDKQKLVSSKSHGPPLSTRSQSATGEDKTAGKASAVKTRAQVEKRKQKKLNIDEAEEFFELISKAQSNRADDQRGLLNKSDLVLPDFLRLDTEADGNNLSSTPISHKTRPRSKENGSALSASHQTQSLDSAGEGKAPRRALMPPNSALRNTSSRIPPPFSSSLSPIPHSQMGGSSGITDWRSDGGVQALEEEESGMDLTFVAEGDITSPNSSLLHQSPIPLPPSPERSHLLKEDTCQDSWARSGTSPV

Target ORF information:

RefSeq Version	XM_009292995.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulator of G protein signaling 12b (rgs12b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292995.3

ORF Insert Sequence:

ATGTGTTTAC AGAGCGTGGA GTCATTTAAC GCTCGACTGT GGAGTCTAAC ACTGCCTTAC 
ATGGAGCCAA CTTTGAGCGT CAATCCTGAT GGATCTGCAG CTGTCTCTGA TGGTGAGCTT 
AACAGTGTGG ACCTGAAAGG CTGCGGCAGT GATAACAGTC TGAACAGCAA TGCCAGCCTA 
CCTAGCGTGC AATGCCATAG ACGGCACACT GAACGGCGCG TTGCATCGTG GGCCGTCTGC 
TTTGAGCGCC TGCTACAGGA TCCCGTCGGT GTCCGATATT TCTCGGAGTT TCTAAAGAAG 
GAGTTTAGCG AAGAGAATAT TTTATTTTGG CAAGCTTGTG AATATTTCAG CCATGTACCT 
GAAACAGACA AGAAACAGCT GTCCCAGCGG GCCAGAGAGA TCTACAACAG CTTCCTGTCT 
AGTAAAGCTA CCACACCCGT CAACATTGAT AGTCAAGCTC AGCTGGCTGA CGACATCCTC 
AACGCACCGA GGCCAGACAT GTTTAAAGAG CAACAGCTTC AGATCTTCAA CCTGATGAAG 
TTTGACAGTT ACACACGCTT CCTAAAGTCT TACCTGTATC AGGAATGTAT GCTTGCAGAG 
GTGGAGGGCC GTCCACTTCC AGACCCCTAC CAGGTGCCCA GTAGCCCCAC ATCCAAACAT 
AGCACAGGTT CCGACCGCTC CAACCTCTCT ACACCTAAAA AGGTGCAGGA CGACAAGAAG 
AGCAAATCGG GAAGGTCACT GAATGAAGAC AGCAGAGATG AACATGGCGA TAAAAAAAAA 
AGTATTTTCT TCTCTTGGTC CAGAAATAGA AGTTTTGGAA AAGGGTCGAA AAAAAGAGAC 
CTCGCAGATT ACAGCTTTAC AGGTAGTAAC GGAAGGAGAG AATCACAAGG CTCTTTGTCG 
TCTGGTGCGA GTTTGGAACT TGCCACTTCC GGTTCTGGAA AAAATGAGGG TGAGGTGTCG 
CGTAACTCCA TGTCGTTGTC TGAGCGTGCC GTGCGTCACT GTAACATTAA CCTACCGGAT 
GGCTCATGCT GCTCAGTACC CATCAGGCCC GGCGTCTCCA TCAGAGAGGT GCTCTTAGGC 
TTGTGTGAGA AACTAGGCAT TAATCTGGCT GCTGTGGACC TGTTCCTAGT AGGAGGAGAA 
AAGCCTTTAG TGTTGGACCA GGACTGCATG ACACTGTGCT CCAGGGATCT GCGGCTGGAG 
AAACGTACGC TATTCCGACT GGATCTGGTG CCTATAAATC GTTCTGTTGG ACTGAAAGCA 
AAACCCACTA AGCCGGTTAC AGAAGTGCTG AGACCAGTGG TGGCTAAATA TGGTTTAAAC 
TTAGGAGACC TGGTGGCCAC GATAAGTGGA GAGAAAGAGC CGCTGGATCT TGGGCTTCCT 
ATATCTAATC TAGATGGCCT GAGGGTGGTT CTTGAAGCAG GTGACCACGT TTCTGGGAAA 
GATAAACAAA AGCTGGTCTC ATCGAAAAGT CACGGTCCAC CTCTGTCTAC ACGAAGCCAA 
TCTGCCACAG GGGAGGACAA AACGGCAGGA AAGGCTTCGG CTGTGAAAAC CAGGGCCCAG 
GTGGAAAAGC GAAAACAGAA AAAGCTTAAT ATAGATGAAG CTGAAGAATT CTTCGAGCTC 
ATATCCAAAG CACAGAGCAA CAGAGCTGAC GACCAGCGGG GTTTGCTGAA CAAATCAGAC 
CTTGTCCTGC CCGACTTCCT GCGCCTCGAC ACGGAAGCAG ACGGCAACAA CCTCAGCTCC 
ACACCCATCT CCCACAAGAC TCGACCACGA TCCAAGGAGA ATGGCTCTGC CCTCAGTGCC 
AGTCACCAAA CGCAGAGCTT AGATTCAGCG GGGGAGGGCA AAGCACCCAG ACGAGCACTA 
ATGCCACCCA ACAGTGCCCT CAGAAACACA TCCAGCCGAA TTCCGCCACC GTTCAGTTCC 
TCGCTCTCCC CAATCCCACA TTCGCAAATG GGGGGCTCGT CCGGGATCAC AGACTGGAGG 
AGTGACGGAG GGGTCCAGGC ACTGGAGGAA GAGGAGAGCG GCATGGACCT GACTTTTGTT 
GCCGAAGGCG ACATCACAAG CCCTAACAGC TCTCTCCTTC ACCAGTCCCC CATTCCTCTC 
CCTCCTTCCC CAGAGCGATC ACACCTCCTG AAGGAGGACA CTTGCCAGGA CAGCTGGGCT 
CGATCAGGTA CCTCTCCTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12b ( XM_009293006.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291281.1 Danio rerio regulator of G-protein signaling 12b (rgs12b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa40342
Clone ID Related Accession (Same CDS sequence)	XM_009293006.2 , XM_009293006.3
Accession Version	XM_009293006.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	regulator of G-protein signaling 12 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTAA AAAAGAGGCT GTCTTTTAAA AGGGTCTGCT ACTTTCATAC GTCTGCAGCT 
GTCTCTGATG GTGAGCTTAA CAGTGTGGAC CTGAAAGGCT GCGGCAGTGA TAACAGTCTG 
AACAGCAATG CCAGCCTACC TAGCGTGCAA TGCCATAGAC GGCACACTGA ACGGCGCGTT 
GCATCGTGGG CCGTCTGCTT TGAGCGCCTG CTACAGGATC CCGTCGGTGT CCGATATTTC 
TCGGAGTTTC TAAAGAAGGA GTTTAGCGAA GAGAATATTT TATTTTGGCA AGCTTGTGAA 
TATTTCAGCC ATGTACCTGA AACAGACAAG AAACAGCTGT CCCAGCGGGC CAGAGAGATC 
TACAACAGCT TCCTGTCTAG TAAAGCTACC ACACCCGTCA ACATTGATAG TCAAGCTCAG 
CTGGCTGACG ACATCCTCAA CGCACCGAGG CCAGACATGT TTAAAGAGCA ACAGCTTCAG 
ATCTTCAACC TGATGAAGTT TGACAGTTAC ACACGCTTCC TAAAGTCTTA CCTGTATCAG 
GAATGTATGC TTGCAGAGGT GGAGGGCCGT CCACTTCCAG ACCCCTACCA GGTGCCCAGT 
AGCCCCACAT CCAAACATAG CACAGGTTCC GACCGCTCCA ACCTCTCTAC ACCTAAAAAG 
GTGCAGGACG ACAAGAAGAG CAAATCGGGA AGGTCACTGA ATGAAGACAG CAGAGATGAA 
CATGGCGATA AAAAAAAAAG TATTTTCTTC TCTTGGTCCA GAAATAGAAG TTTTGGAAAA 
GGGTCGAAAA AAAGAGACCT CGCAGATTAC AGCTTTACAG GTAGTAACGG AAGGAGAGAA 
TCACAAGGCT CTTTGTCGTC TGGTGCGAGT TTGGAACTTG CCACTTCCGG TTCTGGAAAA 
AATGAGGGTG AGGTGTCGCG TAACTCCATG TCGTTGTCTG AGCGTGCCGT GCGTCACTGT 
AACATTAACC TACCGGATGG CTCATGCTGC TCAGTACCCA TCAGGCCCGG CGTCTCCATC 
AGAGAGGTGC TCTTAGGCTT GTGTGAGAAA CTAGGCATTA ATCTGGCTGC TGTGGACCTG 
TTCCTAGTAG GAGGAGAAAA GCCTTTAGTG TTGGACCAGG ACTGCATGAC ACTGTGCTCC 
AGGGATCTGC GGCTGGAGAA ACGTACGCTA TTCCGACTGG ATCTGGTGCC TATAAATCGT 
TCTGTTGGAC TGAAAGCAAA ACCCACTAAG CCGGTTACAG AAGTGCTGAG ACCAGTGGTG 
GCTAAATATG GTTTAAACTT AGGAGACCTG GTGGCCACGA TAAGTGGAGA GAAAGAGCCG 
CTGGATCTTG GGCTTCCTAT ATCTAATCTA GATGGCCTGA GGGTGGTTCT TGAAGCAGGT 
GACCACGTTT CTGGGAAAGA TAAACAAAAG CTGGTCTCAT CGAAAAGTCA CGGTCCACCT 
CTGTCTACAC GAAGCCAATC TGCCACAGGG GAGGACAAAA CGGCAGGAAA GGCTTCGGCT 
GTGAAAACCA GGGCCCAGGT GGAAAAGCGA AAACAGAAAA AGCTTAATAT AGATGAAGCT 
GAAGAATTCT TCGAGCTCAT ATCCAAAGCA CAGAGCAACA GAGCTGACGA CCAGCGGGGT 
TTGCTGAACA AATCAGACCT TGTCCTGCCC GACTTCCTGC GCCTCGACAC GGAAGCAGAC 
GGCAACAACC TCAGCTCCAC ACCCATCTCC CACAAGACTC GACCACGATC CAAGGAGAAT 
GGCTCTGCCC TCAGTGCCAG TCACCAAACG CAGAGCTTAG ATTCAGCGGG GGAGGGCAAA 
GCACCCAGAC GAGCACTAAT GCCACCCAAC AGTGCCCTCA GAAACACATC CAGCCGAATT 
CCGCCACCGT TCAGTTCCTC GCTCTCCCCA ATCCCACATT CGCAAATGGG GGGCTCGTCC 
GGGATCACAG ACTGGAGGAG TGACGGAGGG GTCCAGGCAC TGGAGGAAGA GGAGAGCGGC 
ATGGACCTGA CTTTTGTTGC CGAAGGCGAC ATCACAAGCC CTAACAGCTC TCTCCTTCAC 
CAGTCCCCCA TTCCTCTCCC TCCTTCCCCA GAGCGATCAC ACCTCCTGAA GGAGGACACT 
TGCCAGGACA GCTGGGCTCG ATCAGGTACC TCTCCTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291281.1
CDS	778..2919
Translation	MSLKKRLSFKRVCYFHTSAAVSDGELNSVDLKGCGSDNSLNSNASLPSVQCHRRHTERRVASWAVCFERLLQDPVGVRYFSEFLKKEFSEENILFWQACEYFSHVPETDKKQLSQRAREIYNSFLSSKATTPVNIDSQAQLADDILNAPRPDMFKEQQLQIFNLMKFDSYTRFLKSYLYQECMLAEVEGRPLPDPYQVPSSPTSKHSTGSDRSNLSTPKKVQDDKKSKSGRSLNEDSRDEHGDKKKSIFFSWSRNRSFGKGSKKRDLADYSFTGSNGRRESQGSLSSGASLELATSGSGKNEGEVSRNSMSLSERAVRHCNINLPDGSCCSVPIRPGVSIREVLLGLCEKLGINLAAVDLFLVGGEKPLVLDQDCMTLCSRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLNLGDLVATISGEKEPLDLGLPISNLDGLRVVLEAGDHVSGKDKQKLVSSKSHGPPLSTRSQSATGEDKTAGKASAVKTRAQVEKRKQKKLNIDEAEEFFELISKAQSNRADDQRGLLNKSDLVLPDFLRLDTEADGNNLSSTPISHKTRPRSKENGSALSASHQTQSLDSAGEGKAPRRALMPPNSALRNTSSRIPPPFSSSLSPIPHSQMGGSSGITDWRSDGGVQALEEEESGMDLTFVAEGDITSPNSSLLHQSPIPLPPSPERSHLLKEDTCQDSWARSGTSPV

Target ORF information:

RefSeq Version	XM_009293006.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio regulator of G-protein signaling 12b (rgs12b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293006.2

ORF Insert Sequence:

ATGAGTCTAA AAAAGAGGCT GTCTTTTAAA AGGGTCTGCT ACTTTCATAC GTCTGCAGCT 
GTCTCTGATG GTGAGCTTAA CAGTGTGGAC CTGAAAGGCT GCGGCAGTGA TAACAGTCTG 
AACAGCAATG CCAGCCTACC TAGCGTGCAA TGCCATAGAC GGCACACTGA ACGGCGCGTT 
GCATCGTGGG CCGTCTGCTT TGAGCGCCTG CTACAGGATC CCGTCGGTGT CCGATATTTC 
TCGGAGTTTC TAAAGAAGGA GTTTAGCGAA GAGAATATTT TATTTTGGCA AGCTTGTGAA 
TATTTCAGCC ATGTACCTGA AACAGACAAG AAACAGCTGT CCCAGCGGGC CAGAGAGATC 
TACAACAGCT TCCTGTCTAG TAAAGCTACC ACACCCGTCA ACATTGATAG TCAAGCTCAG 
CTGGCTGACG ACATCCTCAA CGCACCGAGG CCAGACATGT TTAAAGAGCA ACAGCTTCAG 
ATCTTCAACC TGATGAAGTT TGACAGTTAC ACACGCTTCC TAAAGTCTTA CCTGTATCAG 
GAATGTATGC TTGCAGAGGT GGAGGGCCGT CCACTTCCAG ACCCCTACCA GGTGCCCAGT 
AGCCCCACAT CCAAACATAG CACAGGTTCC GACCGCTCCA ACCTCTCTAC ACCTAAAAAG 
GTGCAGGACG ACAAGAAGAG CAAATCGGGA AGGTCACTGA ATGAAGACAG CAGAGATGAA 
CATGGCGATA AAAAAAAAAG TATTTTCTTC TCTTGGTCCA GAAATAGAAG TTTTGGAAAA 
GGGTCGAAAA AAAGAGACCT CGCAGATTAC AGCTTTACAG GTAGTAACGG AAGGAGAGAA 
TCACAAGGCT CTTTGTCGTC TGGTGCGAGT TTGGAACTTG CCACTTCCGG TTCTGGAAAA 
AATGAGGGTG AGGTGTCGCG TAACTCCATG TCGTTGTCTG AGCGTGCCGT GCGTCACTGT 
AACATTAACC TACCGGATGG CTCATGCTGC TCAGTACCCA TCAGGCCCGG CGTCTCCATC 
AGAGAGGTGC TCTTAGGCTT GTGTGAGAAA CTAGGCATTA ATCTGGCTGC TGTGGACCTG 
TTCCTAGTAG GAGGAGAAAA GCCTTTAGTG TTGGACCAGG ACTGCATGAC ACTGTGCTCC 
AGGGATCTGC GGCTGGAGAA ACGTACGCTA TTCCGACTGG ATCTGGTGCC TATAAATCGT 
TCTGTTGGAC TGAAAGCAAA ACCCACTAAG CCGGTTACAG AAGTGCTGAG ACCAGTGGTG 
GCTAAATATG GTTTAAACTT AGGAGACCTG GTGGCCACGA TAAGTGGAGA GAAAGAGCCG 
CTGGATCTTG GGCTTCCTAT ATCTAATCTA GATGGCCTGA GGGTGGTTCT TGAAGCAGGT 
GACCACGTTT CTGGGAAAGA TAAACAAAAG CTGGTCTCAT CGAAAAGTCA CGGTCCACCT 
CTGTCTACAC GAAGCCAATC TGCCACAGGG GAGGACAAAA CGGCAGGAAA GGCTTCGGCT 
GTGAAAACCA GGGCCCAGGT GGAAAAGCGA AAACAGAAAA AGCTTAATAT AGATGAAGCT 
GAAGAATTCT TCGAGCTCAT ATCCAAAGCA CAGAGCAACA GAGCTGACGA CCAGCGGGGT 
TTGCTGAACA AATCAGACCT TGTCCTGCCC GACTTCCTGC GCCTCGACAC GGAAGCAGAC 
GGCAACAACC TCAGCTCCAC ACCCATCTCC CACAAGACTC GACCACGATC CAAGGAGAAT 
GGCTCTGCCC TCAGTGCCAG TCACCAAACG CAGAGCTTAG ATTCAGCGGG GGAGGGCAAA 
GCACCCAGAC GAGCACTAAT GCCACCCAAC AGTGCCCTCA GAAACACATC CAGCCGAATT 
CCGCCACCGT TCAGTTCCTC GCTCTCCCCA ATCCCACATT CGCAAATGGG GGGCTCGTCC 
GGGATCACAG ACTGGAGGAG TGACGGAGGG GTCCAGGCAC TGGAGGAAGA GGAGAGCGGC 
ATGGACCTGA CTTTTGTTGC CGAAGGCGAC ATCACAAGCC CTAACAGCTC TCTCCTTCAC 
CAGTCCCCCA TTCCTCTCCC TCCTTCCCCA GAGCGATCAC ACCTCCTGAA GGAGGACACT 
TGCCAGGACA GCTGGGCTCG ATCAGGTACC TCTCCTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12b ( XM_009293006.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291281.1 Danio rerio regulator of G protein signaling 12b (rgs12b), transcript variant X6, mRNA.

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CloneID	ODa40342
Clone ID Related Accession (Same CDS sequence)	XM_009293006.2 , XM_009293006.3
Accession Version	XM_009293006.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	regulator of G-protein signaling 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTAA AAAAGAGGCT GTCTTTTAAA AGGGTCTGCT ACTTTCATAC GTCTGCAGCT 
GTCTCTGATG GTGAGCTTAA CAGTGTGGAC CTGAAAGGCT GCGGCAGTGA TAACAGTCTG 
AACAGCAATG CCAGCCTACC TAGCGTGCAA TGCCATAGAC GGCACACTGA ACGGCGCGTT 
GCATCGTGGG CCGTCTGCTT TGAGCGCCTG CTACAGGATC CCGTCGGTGT CCGATATTTC 
TCGGAGTTTC TAAAGAAGGA GTTTAGCGAA GAGAATATTT TATTTTGGCA AGCTTGTGAA 
TATTTCAGCC ATGTACCTGA AACAGACAAG AAACAGCTGT CCCAGCGGGC CAGAGAGATC 
TACAACAGCT TCCTGTCTAG TAAAGCTACC ACACCCGTCA ACATTGATAG TCAAGCTCAG 
CTGGCTGACG ACATCCTCAA CGCACCGAGG CCAGACATGT TTAAAGAGCA ACAGCTTCAG 
ATCTTCAACC TGATGAAGTT TGACAGTTAC ACACGCTTCC TAAAGTCTTA CCTGTATCAG 
GAATGTATGC TTGCAGAGGT GGAGGGCCGT CCACTTCCAG ACCCCTACCA GGTGCCCAGT 
AGCCCCACAT CCAAACATAG CACAGGTTCC GACCGCTCCA ACCTCTCTAC ACCTAAAAAG 
GTGCAGGACG ACAAGAAGAG CAAATCGGGA AGGTCACTGA ATGAAGACAG CAGAGATGAA 
CATGGCGATA AAAAAAAAAG TATTTTCTTC TCTTGGTCCA GAAATAGAAG TTTTGGAAAA 
GGGTCGAAAA AAAGAGACCT CGCAGATTAC AGCTTTACAG GTAGTAACGG AAGGAGAGAA 
TCACAAGGCT CTTTGTCGTC TGGTGCGAGT TTGGAACTTG CCACTTCCGG TTCTGGAAAA 
AATGAGGGTG AGGTGTCGCG TAACTCCATG TCGTTGTCTG AGCGTGCCGT GCGTCACTGT 
AACATTAACC TACCGGATGG CTCATGCTGC TCAGTACCCA TCAGGCCCGG CGTCTCCATC 
AGAGAGGTGC TCTTAGGCTT GTGTGAGAAA CTAGGCATTA ATCTGGCTGC TGTGGACCTG 
TTCCTAGTAG GAGGAGAAAA GCCTTTAGTG TTGGACCAGG ACTGCATGAC ACTGTGCTCC 
AGGGATCTGC GGCTGGAGAA ACGTACGCTA TTCCGACTGG ATCTGGTGCC TATAAATCGT 
TCTGTTGGAC TGAAAGCAAA ACCCACTAAG CCGGTTACAG AAGTGCTGAG ACCAGTGGTG 
GCTAAATATG GTTTAAACTT AGGAGACCTG GTGGCCACGA TAAGTGGAGA GAAAGAGCCG 
CTGGATCTTG GGCTTCCTAT ATCTAATCTA GATGGCCTGA GGGTGGTTCT TGAAGCAGGT 
GACCACGTTT CTGGGAAAGA TAAACAAAAG CTGGTCTCAT CGAAAAGTCA CGGTCCACCT 
CTGTCTACAC GAAGCCAATC TGCCACAGGG GAGGACAAAA CGGCAGGAAA GGCTTCGGCT 
GTGAAAACCA GGGCCCAGGT GGAAAAGCGA AAACAGAAAA AGCTTAATAT AGATGAAGCT 
GAAGAATTCT TCGAGCTCAT ATCCAAAGCA CAGAGCAACA GAGCTGACGA CCAGCGGGGT 
TTGCTGAACA AATCAGACCT TGTCCTGCCC GACTTCCTGC GCCTCGACAC GGAAGCAGAC 
GGCAACAACC TCAGCTCCAC ACCCATCTCC CACAAGACTC GACCACGATC CAAGGAGAAT 
GGCTCTGCCC TCAGTGCCAG TCACCAAACG CAGAGCTTAG ATTCAGCGGG GGAGGGCAAA 
GCACCCAGAC GAGCACTAAT GCCACCCAAC AGTGCCCTCA GAAACACATC CAGCCGAATT 
CCGCCACCGT TCAGTTCCTC GCTCTCCCCA ATCCCACATT CGCAAATGGG GGGCTCGTCC 
GGGATCACAG ACTGGAGGAG TGACGGAGGG GTCCAGGCAC TGGAGGAAGA GGAGAGCGGC 
ATGGACCTGA CTTTTGTTGC CGAAGGCGAC ATCACAAGCC CTAACAGCTC TCTCCTTCAC 
CAGTCCCCCA TTCCTCTCCC TCCTTCCCCA GAGCGATCAC ACCTCCTGAA GGAGGACACT 
TGCCAGGACA GCTGGGCTCG ATCAGGTACC TCTCCTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291281.1
CDS	576..2717
Translation	MSLKKRLSFKRVCYFHTSAAVSDGELNSVDLKGCGSDNSLNSNASLPSVQCHRRHTERRVASWAVCFERLLQDPVGVRYFSEFLKKEFSEENILFWQACEYFSHVPETDKKQLSQRAREIYNSFLSSKATTPVNIDSQAQLADDILNAPRPDMFKEQQLQIFNLMKFDSYTRFLKSYLYQECMLAEVEGRPLPDPYQVPSSPTSKHSTGSDRSNLSTPKKVQDDKKSKSGRSLNEDSRDEHGDKKKSIFFSWSRNRSFGKGSKKRDLADYSFTGSNGRRESQGSLSSGASLELATSGSGKNEGEVSRNSMSLSERAVRHCNINLPDGSCCSVPIRPGVSIREVLLGLCEKLGINLAAVDLFLVGGEKPLVLDQDCMTLCSRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLNLGDLVATISGEKEPLDLGLPISNLDGLRVVLEAGDHVSGKDKQKLVSSKSHGPPLSTRSQSATGEDKTAGKASAVKTRAQVEKRKQKKLNIDEAEEFFELISKAQSNRADDQRGLLNKSDLVLPDFLRLDTEADGNNLSSTPISHKTRPRSKENGSALSASHQTQSLDSAGEGKAPRRALMPPNSALRNTSSRIPPPFSSSLSPIPHSQMGGSSGITDWRSDGGVQALEEEESGMDLTFVAEGDITSPNSSLLHQSPIPLPPSPERSHLLKEDTCQDSWARSGTSPV

Target ORF information:

RefSeq Version	XM_009293006.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulator of G protein signaling 12b (rgs12b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293006.3

ORF Insert Sequence:

ATGAGTCTAA AAAAGAGGCT GTCTTTTAAA AGGGTCTGCT ACTTTCATAC GTCTGCAGCT 
GTCTCTGATG GTGAGCTTAA CAGTGTGGAC CTGAAAGGCT GCGGCAGTGA TAACAGTCTG 
AACAGCAATG CCAGCCTACC TAGCGTGCAA TGCCATAGAC GGCACACTGA ACGGCGCGTT 
GCATCGTGGG CCGTCTGCTT TGAGCGCCTG CTACAGGATC CCGTCGGTGT CCGATATTTC 
TCGGAGTTTC TAAAGAAGGA GTTTAGCGAA GAGAATATTT TATTTTGGCA AGCTTGTGAA 
TATTTCAGCC ATGTACCTGA AACAGACAAG AAACAGCTGT CCCAGCGGGC CAGAGAGATC 
TACAACAGCT TCCTGTCTAG TAAAGCTACC ACACCCGTCA ACATTGATAG TCAAGCTCAG 
CTGGCTGACG ACATCCTCAA CGCACCGAGG CCAGACATGT TTAAAGAGCA ACAGCTTCAG 
ATCTTCAACC TGATGAAGTT TGACAGTTAC ACACGCTTCC TAAAGTCTTA CCTGTATCAG 
GAATGTATGC TTGCAGAGGT GGAGGGCCGT CCACTTCCAG ACCCCTACCA GGTGCCCAGT 
AGCCCCACAT CCAAACATAG CACAGGTTCC GACCGCTCCA ACCTCTCTAC ACCTAAAAAG 
GTGCAGGACG ACAAGAAGAG CAAATCGGGA AGGTCACTGA ATGAAGACAG CAGAGATGAA 
CATGGCGATA AAAAAAAAAG TATTTTCTTC TCTTGGTCCA GAAATAGAAG TTTTGGAAAA 
GGGTCGAAAA AAAGAGACCT CGCAGATTAC AGCTTTACAG GTAGTAACGG AAGGAGAGAA 
TCACAAGGCT CTTTGTCGTC TGGTGCGAGT TTGGAACTTG CCACTTCCGG TTCTGGAAAA 
AATGAGGGTG AGGTGTCGCG TAACTCCATG TCGTTGTCTG AGCGTGCCGT GCGTCACTGT 
AACATTAACC TACCGGATGG CTCATGCTGC TCAGTACCCA TCAGGCCCGG CGTCTCCATC 
AGAGAGGTGC TCTTAGGCTT GTGTGAGAAA CTAGGCATTA ATCTGGCTGC TGTGGACCTG 
TTCCTAGTAG GAGGAGAAAA GCCTTTAGTG TTGGACCAGG ACTGCATGAC ACTGTGCTCC 
AGGGATCTGC GGCTGGAGAA ACGTACGCTA TTCCGACTGG ATCTGGTGCC TATAAATCGT 
TCTGTTGGAC TGAAAGCAAA ACCCACTAAG CCGGTTACAG AAGTGCTGAG ACCAGTGGTG 
GCTAAATATG GTTTAAACTT AGGAGACCTG GTGGCCACGA TAAGTGGAGA GAAAGAGCCG 
CTGGATCTTG GGCTTCCTAT ATCTAATCTA GATGGCCTGA GGGTGGTTCT TGAAGCAGGT 
GACCACGTTT CTGGGAAAGA TAAACAAAAG CTGGTCTCAT CGAAAAGTCA CGGTCCACCT 
CTGTCTACAC GAAGCCAATC TGCCACAGGG GAGGACAAAA CGGCAGGAAA GGCTTCGGCT 
GTGAAAACCA GGGCCCAGGT GGAAAAGCGA AAACAGAAAA AGCTTAATAT AGATGAAGCT 
GAAGAATTCT TCGAGCTCAT ATCCAAAGCA CAGAGCAACA GAGCTGACGA CCAGCGGGGT 
TTGCTGAACA AATCAGACCT TGTCCTGCCC GACTTCCTGC GCCTCGACAC GGAAGCAGAC 
GGCAACAACC TCAGCTCCAC ACCCATCTCC CACAAGACTC GACCACGATC CAAGGAGAAT 
GGCTCTGCCC TCAGTGCCAG TCACCAAACG CAGAGCTTAG ATTCAGCGGG GGAGGGCAAA 
GCACCCAGAC GAGCACTAAT GCCACCCAAC AGTGCCCTCA GAAACACATC CAGCCGAATT 
CCGCCACCGT TCAGTTCCTC GCTCTCCCCA ATCCCACATT CGCAAATGGG GGGCTCGTCC 
GGGATCACAG ACTGGAGGAG TGACGGAGGG GTCCAGGCAC TGGAGGAAGA GGAGAGCGGC 
ATGGACCTGA CTTTTGTTGC CGAAGGCGAC ATCACAAGCC CTAACAGCTC TCTCCTTCAC 
CAGTCCCCCA TTCCTCTCCC TCCTTCCCCA GAGCGATCAC ACCTCCTGAA GGAGGACACT 
TGCCAGGACA GCTGGGCTCG ATCAGGTACC TCTCCTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12b ( XM_009292984.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291259.1 Danio rerio regulator of G protein signaling 12b (rgs12b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa40339
Clone ID Related Accession (Same CDS sequence)	XM_009292984.2
Accession Version	XM_009292984.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	regulator of G-protein signaling 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGGC AATCAGAACA AGGCAGGAGG CGTGTAAATG CCCAGCCATC TGCTCGCATT 
AGAGGAGTGG AGGTTGCAAG GGGGAGAACA GGCTATGGCT TCACACTCTC TGGTCAGTCT 
CCATGTGTTC TGAATTGCAT CCTTAAGGGT AGTCCAGCTG ATTATGTGGG CCTTCGCTCT 
GGAGATCAGA TCCTGAGTGT GAATGACATT AATGTGTCCA AAGCTTCTCA TGAGGATGTT 
GTTAAGCTGA TTGGCCGCTG CACAGGTGTC CTACACTTAG TCATTGCCGA AGGCAGTCAT 
AAAAACGGCC ACGTTGATTC GTGTTCAAGT GATGAAGAAC TGGAGTTCCA TGACCACAAG 
AGCCACTGGC TGAGGCCAAA GATGGACTCA AAGACACTAG GCATAAACCG TGCAGAACGT 
GTTGTAGCAG AGATGCAGTC TGGTGGGATC TTCAACATGA TCTTTGAAAA TTCGAGCCAC 
TCATCCAGCA GCTCAGAAAG AGCAGTTGCT TGTGCCTCTG CTTCCAAACC AAGGGCTGTC 
TCAGAGCCAG AATTCCCTTA TGTGAGCGTC AACACCCAAA AAAGTGATCC CAACTTGCTC 
ACTGAGGAAG AGATGGCCAA AGTGCTCAAT GACGACTCTG TGTTTGTTGA TATGTTTGAA 
CCGCAAGATG AATTTGCCCT GTTGCAAACT GCAGACTCTG CAGGAATACT CAATGTTGGG 
ATGATTGTGG GTTACTTGGG ATCTATTGAA TTAGCTTCAA CAAGTGCCAA TTTAGAAAGT 
GACAGCCTGC AGGCAATACG TGGCTGTATG CGACGTCTAC GTGCCGAACA AAAAGTGCAC 
TCGTTGGTTT TGCTTAAAGT CATGCATGAT TGTGTGCAAC TATGCAATGA CCGAGGTGCT 
GTTCTAGCAA CTTACCCTGC AGAGAAGTTA GCCTTCAGTG CTGTGTGTCC AGACGACCGC 
CGCTTTTTTG GCCTTGTCAC CATGCAAGCA ACAAATGACC ACCGCATGTA TAGGCATGGT 
GAGGAGGAGC AAGGCTTACG GACATCTTGT CATGTCTTCA TTGTAGACCC GGAACTCTGT 
CATCATAAAA TCCACCAGGG TATTGCCCGA CGCTTTGGCT TTGACTGCAC TCCAGACCCA 
GATACTGGTG GCTGCTTGGA GTTCCCACCG ACTTCTCAGC CACTTCTACA GTTTGTGTCT 
GTTCTGTATC GTGACATGGG AGAATCCATA GAGGGTATGC GAGCTCGAGC TTTCCTTGAT 
GGTGACTTAG ATGCTCAGCA GAACAACAGC ACAAGCAGCA ACAGTGACAG CGGCATTGGG 
AACTTCTTGC CAGAGGAAAA GAACAACCGA GTCCTCTTGG TGGACTTGGG TGCAAATCCT 
AGTCGACATG TTCCTACAGG ACCTTGGGAA AACCCTATGG GATCTTTTTG CCAGGGTGCC 
ATAGGAGGTC TTCCGCCACC ACCTCCTCCG CCACCGATCT CACATCGTAA CGGATTTCGG 
CATGAGCAGT TTGTGGATCA CCCTCAGCCC CATCCACATT CCCATGGAGA CCCACCTCTG 
CTTTCTGGCA GGCATCTAAA CTCCTCCTCA CGGCTGGATG TGTCAGGCCT TCCTTCGCGC 
TCACACTATT CTGCTCATGG CAAGAAAGCG GCAGGGGCAT CAGGCCAGCG CTGGCTGCCA 
GTTCACGTCC TGAGGGATTG GCGTCAGGGA CACAGCAGTG ATCAGGAGTC TTATGCAGAG 
TCCACAGACG GATGGTCCAG TGCTAACTGT AGCACCCTGC CACCACCGAT GAGCAAGATT 
CCAGCTGACC GGTACCGTGC TGCAGGCGAA ATCGCCTCAC AGCCTCACCG GCTTCATGCA 
CAGAAGGATG AGTGGGCTAA GAAGCTGTTT GGGGAGAAGA ACCTACGAGA GCATTCACAA 
CAATCGAACA ATGTCAGAAG AGCTAAAGAT TCAGAAAAAA AGGGTGGAAG GTTCAAAGGG 
ATCACTATGG GCTTTCCTCA GCGTTCCTCT GGCCGACGAT CTTTTGGACG TTCCAAGCGT 
CTTAGCATGG CTCGCTCACT TGATGACCTT GAGTCTGCAG CTGTCTCTGA TGGTGAGCTT 
AACAGTGTGG ACCTGAAAGG CTGCGGCAGT GATAACAGTC TGAACAGCAA TGCCAGCCTA 
CCTAGCGTGC AATGCCATAG ACGGCACACT GAACGGCGCG TTGCATCGTG GGCCGTCTGC 
TTTGAGCGCC TGCTACAGGA TCCCGTCGGT GTCCGATATT TCTCGGAGTT TCTAAAGAAG 
GAGTTTAGCG AAGAGAATAT TTTATTTTGG CAAGCTTGTG AATATTTCAG CCATGTACCT 
GAAACAGACA AGAAACAGCT GTCCCAGCGG GCCAGAGAGA TCTACAACAG CTTCCTGTCT 
AGTAAAGCTA CCACACCCGT CAACATTGAT AGTCAAGCTC AGCTGGCTGA CGACATCCTC 
AACGCACCGA GGCCAGACAT GTTTAAAGAG CAACAGCTTC AGATCTTCAA CCTGATGAAG 
TTTGACAGTT ACACACGCTT CCTAAAGTCT TACCTGTATC AGGAATGTAT GCTTGCAGAG 
GTGGAGGGCC GTCCACTTCC AGACCCCTAC CAGGTGCCCA GTAGCCCCAC ATCCAAACAT 
AGCACAGGTT CCGACCGCTC CAACCTCTCT ACACCTAAAA AGGTGCAGGA CGACAAGAAG 
AGCAAATCGG GAAGGTCACT GAATGAAGAC AGCAGAGATG AACATGGCGA TAAAAAAAAA 
AGTATTTTCT TCTCTTGGTC CAGAAATAGA AGTTTTGGAA AAGGGTCGAA AAAAAGAGAC 
CTCGCAGATT ACAGCTTTAC AGGTAGTAAC GGAAGGAGAG AATCACAAGG CTCTTTGTCG 
TCTGGTGCGA GTTTGGAACT TGCCACTTCC GGTTCTGGAA AAAATGAGGG TGAGGTGTCG 
CGTAACTCCA TGTCGTTGTC TGAGCGTGCC GTGCGTCACT GTAACATTAA CCTACCGGAT 
GGCTCATGCT GCTCAGTACC CATCAGGCCC GGCGTCTCCA TCAGAGAGGT GCTCTTAGGC 
TTGTGTGAGA AACTAGGCAT TAATCTGGCT GCTGTGGACC TGTTCCTAGT AGGAGGAGAA 
AAGCCTTTAG TGTTGGACCA GGACTGCATG ACACTGTGCT CCAGGGATCT GCGGCTGGAG 
AAACGTACGC TATTCCGACT GGATCTGGTG CCTATAAATC GTTCTGTTGG ACTGAAAGCA 
AAACCCACTA AGCCGGTTAC AGAAGTGCTG AGACCAGTGG TGGCTAAATA TGGTTTAAAC 
TTAGGAGACC TGGTGGCCAC GATAAGTGGA GAGAAAGAGC CGCTGGATCT TGGGCTTCCT 
ATATCTAATC TAGATGGCCT GAGGGTGGTT CTTGAAGCAG GTGACCACGT TTCTGGGAAA 
GATAAACAAA AGCTGGTCTC ATCGAAAAGT CACGGTCCAC CTCTGTCTAC ACGAAGCCAA 
TCTGCCACAA ATTCTTCGAG CTCATATCCA AAGCACAGAG CAACAGAGCT GACGACCAGC 
GGGGTTTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291259.1
CDS	356..3907
Translation	MFRQSEQGRRRVNAQPSARIRGVEVARGRTGYGFTLSGQSPCVLNCILKGSPADYVGLRSGDQILSVNDINVSKASHEDVVKLIGRCTGVLHLVIAEGSHKNGHVDSCSSDEELEFHDHKSHWLRPKMDSKTLGINRAERVVAEMQSGGIFNMIFENSSHSSSSSERAVACASASKPRAVSEPEFPYVSVNTQKSDPNLLTEEEMAKVLNDDSVFVDMFEPQDEFALLQTADSAGILNVGMIVGYLGSIELASTSANLESDSLQAIRGCMRRLRAEQKVHSLVLLKVMHDCVQLCNDRGAVLATYPAEKLAFSAVCPDDRRFFGLVTMQATNDHRMYRHGEEEQGLRTSCHVFIVDPELCHHKIHQGIARRFGFDCTPDPDTGGCLEFPPTSQPLLQFVSVLYRDMGESIEGMRARAFLDGDLDAQQNNSTSSNSDSGIGNFLPEEKNNRVLLVDLGANPSRHVPTGPWENPMGSFCQGAIGGLPPPPPPPPISHRNGFRHEQFVDHPQPHPHSHGDPPLLSGRHLNSSSRLDVSGLPSRSHYSAHGKKAAGASGQRWLPVHVLRDWRQGHSSDQESYAESTDGWSSANCSTLPPPMSKIPADRYRAAGEIASQPHRLHAQKDEWAKKLFGEKNLREHSQQSNNVRRAKDSEKKGGRFKGITMGFPQRSSGRRSFGRSKRLSMARSLDDLESAAVSDGELNSVDLKGCGSDNSLNSNASLPSVQCHRRHTERRVASWAVCFERLLQDPVGVRYFSEFLKKEFSEENILFWQACEYFSHVPETDKKQLSQRAREIYNSFLSSKATTPVNIDSQAQLADDILNAPRPDMFKEQQLQIFNLMKFDSYTRFLKSYLYQECMLAEVEGRPLPDPYQVPSSPTSKHSTGSDRSNLSTPKKVQDDKKSKSGRSLNEDSRDEHGDKKKSIFFSWSRNRSFGKGSKKRDLADYSFTGSNGRRESQGSLSSGASLELATSGSGKNEGEVSRNSMSLSERAVRHCNINLPDGSCCSVPIRPGVSIREVLLGLCEKLGINLAAVDLFLVGGEKPLVLDQDCMTLCSRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLNLGDLVATISGEKEPLDLGLPISNLDGLRVVLEAGDHVSGKDKQKLVSSKSHGPPLSTRSQSATNSSSSYPKHRATELTTSGVC

Target ORF information:

RefSeq Version	XM_009292984.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulator of G protein signaling 12b (rgs12b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292984.2

ORF Insert Sequence:

ATGTTCAGGC AATCAGAACA AGGCAGGAGG CGTGTAAATG CCCAGCCATC TGCTCGCATT 
AGAGGAGTGG AGGTTGCAAG GGGGAGAACA GGCTATGGCT TCACACTCTC TGGTCAGTCT 
CCATGTGTTC TGAATTGCAT CCTTAAGGGT AGTCCAGCTG ATTATGTGGG CCTTCGCTCT 
GGAGATCAGA TCCTGAGTGT GAATGACATT AATGTGTCCA AAGCTTCTCA TGAGGATGTT 
GTTAAGCTGA TTGGCCGCTG CACAGGTGTC CTACACTTAG TCATTGCCGA AGGCAGTCAT 
AAAAACGGCC ACGTTGATTC GTGTTCAAGT GATGAAGAAC TGGAGTTCCA TGACCACAAG 
AGCCACTGGC TGAGGCCAAA GATGGACTCA AAGACACTAG GCATAAACCG TGCAGAACGT 
GTTGTAGCAG AGATGCAGTC TGGTGGGATC TTCAACATGA TCTTTGAAAA TTCGAGCCAC 
TCATCCAGCA GCTCAGAAAG AGCAGTTGCT TGTGCCTCTG CTTCCAAACC AAGGGCTGTC 
TCAGAGCCAG AATTCCCTTA TGTGAGCGTC AACACCCAAA AAAGTGATCC CAACTTGCTC 
ACTGAGGAAG AGATGGCCAA AGTGCTCAAT GACGACTCTG TGTTTGTTGA TATGTTTGAA 
CCGCAAGATG AATTTGCCCT GTTGCAAACT GCAGACTCTG CAGGAATACT CAATGTTGGG 
ATGATTGTGG GTTACTTGGG ATCTATTGAA TTAGCTTCAA CAAGTGCCAA TTTAGAAAGT 
GACAGCCTGC AGGCAATACG TGGCTGTATG CGACGTCTAC GTGCCGAACA AAAAGTGCAC 
TCGTTGGTTT TGCTTAAAGT CATGCATGAT TGTGTGCAAC TATGCAATGA CCGAGGTGCT 
GTTCTAGCAA CTTACCCTGC AGAGAAGTTA GCCTTCAGTG CTGTGTGTCC AGACGACCGC 
CGCTTTTTTG GCCTTGTCAC CATGCAAGCA ACAAATGACC ACCGCATGTA TAGGCATGGT 
GAGGAGGAGC AAGGCTTACG GACATCTTGT CATGTCTTCA TTGTAGACCC GGAACTCTGT 
CATCATAAAA TCCACCAGGG TATTGCCCGA CGCTTTGGCT TTGACTGCAC TCCAGACCCA 
GATACTGGTG GCTGCTTGGA GTTCCCACCG ACTTCTCAGC CACTTCTACA GTTTGTGTCT 
GTTCTGTATC GTGACATGGG AGAATCCATA GAGGGTATGC GAGCTCGAGC TTTCCTTGAT 
GGTGACTTAG ATGCTCAGCA GAACAACAGC ACAAGCAGCA ACAGTGACAG CGGCATTGGG 
AACTTCTTGC CAGAGGAAAA GAACAACCGA GTCCTCTTGG TGGACTTGGG TGCAAATCCT 
AGTCGACATG TTCCTACAGG ACCTTGGGAA AACCCTATGG GATCTTTTTG CCAGGGTGCC 
ATAGGAGGTC TTCCGCCACC ACCTCCTCCG CCACCGATCT CACATCGTAA CGGATTTCGG 
CATGAGCAGT TTGTGGATCA CCCTCAGCCC CATCCACATT CCCATGGAGA CCCACCTCTG 
CTTTCTGGCA GGCATCTAAA CTCCTCCTCA CGGCTGGATG TGTCAGGCCT TCCTTCGCGC 
TCACACTATT CTGCTCATGG CAAGAAAGCG GCAGGGGCAT CAGGCCAGCG CTGGCTGCCA 
GTTCACGTCC TGAGGGATTG GCGTCAGGGA CACAGCAGTG ATCAGGAGTC TTATGCAGAG 
TCCACAGACG GATGGTCCAG TGCTAACTGT AGCACCCTGC CACCACCGAT GAGCAAGATT 
CCAGCTGACC GGTACCGTGC TGCAGGCGAA ATCGCCTCAC AGCCTCACCG GCTTCATGCA 
CAGAAGGATG AGTGGGCTAA GAAGCTGTTT GGGGAGAAGA ACCTACGAGA GCATTCACAA 
CAATCGAACA ATGTCAGAAG AGCTAAAGAT TCAGAAAAAA AGGGTGGAAG GTTCAAAGGG 
ATCACTATGG GCTTTCCTCA GCGTTCCTCT GGCCGACGAT CTTTTGGACG TTCCAAGCGT 
CTTAGCATGG CTCGCTCACT TGATGACCTT GAGTCTGCAG CTGTCTCTGA TGGTGAGCTT 
AACAGTGTGG ACCTGAAAGG CTGCGGCAGT GATAACAGTC TGAACAGCAA TGCCAGCCTA 
CCTAGCGTGC AATGCCATAG ACGGCACACT GAACGGCGCG TTGCATCGTG GGCCGTCTGC 
TTTGAGCGCC TGCTACAGGA TCCCGTCGGT GTCCGATATT TCTCGGAGTT TCTAAAGAAG 
GAGTTTAGCG AAGAGAATAT TTTATTTTGG CAAGCTTGTG AATATTTCAG CCATGTACCT 
GAAACAGACA AGAAACAGCT GTCCCAGCGG GCCAGAGAGA TCTACAACAG CTTCCTGTCT 
AGTAAAGCTA CCACACCCGT CAACATTGAT AGTCAAGCTC AGCTGGCTGA CGACATCCTC 
AACGCACCGA GGCCAGACAT GTTTAAAGAG CAACAGCTTC AGATCTTCAA CCTGATGAAG 
TTTGACAGTT ACACACGCTT CCTAAAGTCT TACCTGTATC AGGAATGTAT GCTTGCAGAG 
GTGGAGGGCC GTCCACTTCC AGACCCCTAC CAGGTGCCCA GTAGCCCCAC ATCCAAACAT 
AGCACAGGTT CCGACCGCTC CAACCTCTCT ACACCTAAAA AGGTGCAGGA CGACAAGAAG 
AGCAAATCGG GAAGGTCACT GAATGAAGAC AGCAGAGATG AACATGGCGA TAAAAAAAAA 
AGTATTTTCT TCTCTTGGTC CAGAAATAGA AGTTTTGGAA AAGGGTCGAA AAAAAGAGAC 
CTCGCAGATT ACAGCTTTAC AGGTAGTAAC GGAAGGAGAG AATCACAAGG CTCTTTGTCG 
TCTGGTGCGA GTTTGGAACT TGCCACTTCC GGTTCTGGAA AAAATGAGGG TGAGGTGTCG 
CGTAACTCCA TGTCGTTGTC TGAGCGTGCC GTGCGTCACT GTAACATTAA CCTACCGGAT 
GGCTCATGCT GCTCAGTACC CATCAGGCCC GGCGTCTCCA TCAGAGAGGT GCTCTTAGGC 
TTGTGTGAGA AACTAGGCAT TAATCTGGCT GCTGTGGACC TGTTCCTAGT AGGAGGAGAA 
AAGCCTTTAG TGTTGGACCA GGACTGCATG ACACTGTGCT CCAGGGATCT GCGGCTGGAG 
AAACGTACGC TATTCCGACT GGATCTGGTG CCTATAAATC GTTCTGTTGG ACTGAAAGCA 
AAACCCACTA AGCCGGTTAC AGAAGTGCTG AGACCAGTGG TGGCTAAATA TGGTTTAAAC 
TTAGGAGACC TGGTGGCCAC GATAAGTGGA GAGAAAGAGC CGCTGGATCT TGGGCTTCCT 
ATATCTAATC TAGATGGCCT GAGGGTGGTT CTTGAAGCAG GTGACCACGT TTCTGGGAAA 
GATAAACAAA AGCTGGTCTC ATCGAAAAGT CACGGTCCAC CTCTGTCTAC ACGAAGCCAA 
TCTGCCACAA ATTCTTCGAG CTCATATCCA AAGCACAGAG CAACAGAGCT GACGACCAGC 
GGGGTTTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12b ( NM_214724.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_999889.1 Danio rerio regulator of G protein signaling 12b (rgs12b), mRNA.

Price & Availability↑

CloneID	ODa36013
Clone ID Related Accession (Same CDS sequence)	NM_214724.1
Accession Version	NM_214724.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	regulator of G-protein signaling 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY427669.1. ##Evidence-Data-START## Transcript exon combination :: AY427669.1, BC163722.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTCAGGC AATCAGAACA AGGCAGGAGG CGTGTAAATG CCCAGCCATC TGCTCGCATT 
AGAGGAGTGG AGGTTGCAAG GGGGAGAACA GGTTATGGCT TCACACTCTC TGGTCAGTCT 
CCATGTGTTC TGAATTGCAT CCTCAAGGGT AGTCCAGCTG ATTATGTGGG CCTTCGCTCT 
GGAGATCAGA TCCTGAGTGT GAATGACATT AATGTGTCCA AAGCTTCTCA TGAGGATGTT 
GTTAAGCTGA TTGGCCGCTG CACAGGTGTC CTACACTTAG TCATTGCCGA AGGCAGTCAT 
AAAAACGGCC ATGTTGATTC GTGTTCAAGT GATGAAGAAC TGGAGTTCCA TGACCACAAG 
AGCCACTGGC TGAGGCCAAA GATGGACTCA AAGACACTAG GCATAAACCG TGCAGAACGT 
GTTGTAGCAG AGATGCAGTC TGGTGGGATC TTCAACATGA TCTTTGAAAA TTCAAGCCAC 
TCATCCAGCA GCTCAGAAAG AGCAGTTGCT TGTGCCTCTG CTTCCAAGCC AAGGGCTGTC 
TCAGAGCCAG AATTCCCTTA TGTGAGCGTC AACACCCAAA AAAGTGATCC CAACTTGCTC 
ACTGAGGAAG AGATGGCCAA AGTGCTCAAT GACGACTCTG TGTTTGTTGA TATGTTTGAA 
CCGCAAGATG AATTTGCCCT GTTGCAAACT GCAGACTCTG CAGGAATACT CAATGTTGGG 
ATGATTGTGG GTTACTTGGG ATCTATTGAA TTAGCTTCAA CAAGTGCCAA TTTAGAAAGT 
GACAGCCTGC AGGCAATCCG TGGCTGTATG CGACGTCTAC GTGCCGAACA AAAAGTGCAC 
TCGTTGGTTT TGCTTAAAGT CATGCATGAT TGTGTGCAAC TATGCAATGA CCGAGGTGCT 
GTTCTAGCAA CTTACCCCGC TGAGAAGTTA GCCTTCAGTG CTGTGTGTCC AGACGACCGC 
CGCTTTTTTG GCCTTGTCAC CATGCAAGCA ACAAATGACC ACCGCATGTA TAGGCATGGT 
GAGGAGGAGC AAGGCTTACG GACATCTTGT CATGTCTTCA TTGTAGACCC GGAACTCTGT 
CATCATAAAA TCCACCAAGG TATTGCCCGA CGCTTTGGCT TTGACTGCAC TCCAGACCCA 
GATACTGGTG GCTGCTTGGA GTTCCCACCG ACTTCTCAGC CACTTCTACA GTTTGTGTCT 
GTTCTGTATC GTGACATGGG AGAATCCATA GAGGGTATGC GAGCTCGAGC TTTCCTTGAT 
GGTGACTTAG ATGCTCAGCA GAACAACAGC ACAAGCAGCA ACAGTGACAG CGGCATTGGG 
AACTTCTTGC CAGAGGAAAA GAACAACCGA GTCCTCTTGG TGGACTTGGG TGCAAATCCT 
AGTCGACATG TTCCTACAGG ACCTTGGGAA AACCCTATGG GATCTTTTTG CCAGGGTGCC 
ATAGGAGGTC TTCCGCCACC ACCTCCTCCG CCACCGATCT CACATCGTAA CGGATTTCGG 
CATGAGCAGT TTGTGGATCA CCCTCAGCCC CATCCACATT CCCATGGAGA CCCACCTCTG 
CTTTCTGGCA GGCATCTAAA CTCCTCCTCA CGGCTGGATG TGTCAGGCCT TCCTTCGCGC 
TCACACTATT CTGCTCATGG CAAGAAAGCG GCAGGGGCAT CAGGCCAGCG CTGGCTGCCA 
GTTCACGTCC TGAGGGATTG GCGTCAGGGA CACAGCAGTG ATCAGGAGTC TTATGCAGAG 
TCCACAGACG GATGGTCCAG TGCTAACTGT AGCACCCTGC CACCACCGAT GAGCAAGATT 
CCAGCTGACC GGTACCGTGC TGCAGGCGAA ATCGCCTCAC AGCCTCACCG GCTTCATGCA 
CAGAAGGATG AGTGGGCTAA GAAGCTGTTT GGGGAGAAGA ACCTACGAGA GCATTCACAA 
CAATCGAACA ATGTCAGAAG AGCTAAAGAT TCAGAAAAAA AGGGTGGAAG GTTCAAAGGG 
ATCACTATGG GCTTTCCTCA GCGTTCCTCT GGCCGACGAT CTTTTGGACG TTCCAAGCGT 
CTTAGCATGG CTCGCTCACT TGATGACCTT GAGTCTGCAG CTGTCTCTGA TGGTGAGCTT 
AACAGTGTGG ACCTGAAAGG CTGCGGCAGT GATAACAGTC TGAACAGCAA TGCCAGCCTA 
CCTAGCGTGC AATGCCATAG ACGGCACACT GAACGGCGCG TTGCATCGTG GGCCGTCTGC 
TTTGAGCGCC TGCTACAGGA TCCCGTCGGT GTCCGATATT TCTCGGAGTT TCTAAAGAAG 
GAGTTTAGCG AAGAGAATAT TTTATTTTGG CAAGCTTGTG AATATTTCAG CCATGTACCT 
GAAACAGACA AGAAACAGCT GTCCCAGCGG GCCAGAGAGA TCTACAACAG CTTCCTGTCT 
AGCAAAGCTA CCACACCCGT CAACATTGAT AGTCAAGCTC AGCTGGCTGA CGACATCCTC 
AACGCACCGA GGCCAGACAT GTTTAAAGAG CAACAGCTTC AGATCTTCAA CCTGATGAAG 
TTTGACAGTT ACACACGCTT CCTAAAGTCT TACCTGTATC AGGAATGTAT GCTTGCAGAG 
GTGGAGGGCC GTCCACTTCC AGACCCCTAC CAGGTGCCCA GTAGCCCCAC ATCCAAACAT 
AGCACAGGTT CCGACCGCTC CAACCTCTCT ACACCTAAAA AGGTGCAGGA CGACAAGAAG 
AGCAAATCGG GAAGGTCACT GAATGAAGAC AGCAGAGATG AACATGGCGA TAAAAAAAAA 
AGTATTTTCT TCTCTTGGTC CAGAAATAGA AGTTTTGGAA AAGGGTCGAA AAAAAGAGAC 
CTCGCAGATT ACAGCTTTAC AGGTAGTAAC GGAAGGAGAG AATCACAAGG CTCTTTGTCG 
TCTGGTGCGA GTTTGGAACT TGCCACTTCC GGTTCTGGAA AAAATGAGGG TGAGGTGTCG 
CGTAACTCCA TGTCGTTGTC TGAGCGTGCC GTGCGTCACT GTAACATTAA CCTACCGGAT 
GGCTCATGCT GCTCAGTACC CATCAGGCCC GGCGTCTCCA TCAGAGAGGT GCTCTTAGGC 
TTGTGTGAGA AACTAGGCAT TAATCTGGCT GCTGTGGACC TGTTCCTAGT GGGAGGAGAA 
AAGCCTTTAG TGTTGGACCA GGACTGCATG ACACTGTGCT CCAGGGATCT GCGGCTGGAG 
AAACGTACGC TATTCCGACT GGATCTGGTG CCTATAAATC GTTCTGTTGG ACTGAAAGCA 
AAACCCACTA AGCCGGTTAC AGAAGTGCTG AGACCAGTGG TGGCTAAATA TGGTTTAAAC 
TTAGGAGACC TGGTGGCCAC GATAAGTGGA GAGAAAGAGC CGCTGGATCT TGGGCTTCCT 
ATATCTAATC TAGATGGCCT GAGGGTGGTT CTTGAAGCAG GTGACCACGT TTCTGGGAAA 
GATAAACAAA AGCTGGTCTC ATCGAAAAGT CACGGTCCAC CTCTGTCTAC ACGAAGCCAA 
TCTGCCACAG GGGAGGACAA AACGGCAGGA AAGGCTTCGG CTGTGAAAAC CAGGGCCCAG 
GTGGAAAAGC GAAAACAGAA AAAGCTTAAT ATAGATGAAG CCGAAGAATT CTTCGAGCTC 
ATATCCAAAG CACAGAGCAA CAGAGCTGAC GACCAGCGGG GTTTGCTGAA CAAATCAGAC 
CTTGTCCTGC CCGACTTCCT GCGCCTCGAC ACGGAAGCAG ACGGCAACAA CCTCAGCTCC 
ACACCCATCT CCCACAAGAC TCGACCACGA TCCAAGGAGA ATGGCTCTGC CCTCAGTGCC 
AGTCACCAAA CGCAGAGCTT AGATTCAGCG GGGGAGGGCA AAGCACCCAG ACGAGCACTA 
ATGCCACCCA ACAGTGCCCT CAGAAACACA TCCAGCCGAA TTCCGCCACC GTTCAGTTCC 
TCGCTCTCCC CAATCCCACA TTCGCAAATG GGGGGCTCGT CCGGGATCAC AGACTGGAGG 
AGTGACGGAG GGGTCCAGGC ACTGGAGGAA GAGGAGAGCG GCATGGACCT GACTTTTGTT 
GCCGAAGGCG ACATCACAAG CCCTAACAGC TCTCTCCTTC ACCAGTCCCC CATTCCTCTC 
CCTCCTTCCC CAGAGCGATC ACACCTCCTG AAGGAGGACA CTTGCCAGGA CAGCTGGGCT 
CGATCAGGCG GACTTATGAC CAAGACAGTG GTATCAAACA GAAACCGCTT CTCTTCAGTG 
AAAAATGAAA GACGGCAGAG AGACAGGACC AGTGTTTCCA AATATTCCCA CAGGAGGGCA 
CCCTCCGCCC CACCTCCTCC ACCTCCACAT CCACCTCCCG CTCCTCCATC AGCTCCACCC 
AGGGTCAGAG GAGCAGGCAA GCGCTCTCCT CTTCATGTGC GAGGGGCTCT TGACGTTGAT 
GGGATCCATT GTGGTGACCT TGGTGGTGAT AATAGAGAAT CCGAGGGCTT GGAGAGGGTG 
GTTGGTGATC TGAAAACCAG CAAAGGAAGA ATGAGAACAG GAGTTTATCA GACCTCAGAC 
CAAGCTGAAC TGCTGCCTAC GAAAAAGCGC ATTGATCCAG TGGCAAAAAA ATCAATCAAG 
CCTTCAGTTA AAACGTCAAG CAAGAAGAGC AACCACAGCA AAAACAATGC CACTTTTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999889.1
CDS	176..4798
Translation	MFRQSEQGRRRVNAQPSARIRGVEVARGRTGYGFTLSGQSPCVLNCILKGSPADYVGLRSGDQILSVNDINVSKASHEDVVKLIGRCTGVLHLVIAEGSHKNGHVDSCSSDEELEFHDHKSHWLRPKMDSKTLGINRAERVVAEMQSGGIFNMIFENSSHSSSSSERAVACASASKPRAVSEPEFPYVSVNTQKSDPNLLTEEEMAKVLNDDSVFVDMFEPQDEFALLQTADSAGILNVGMIVGYLGSIELASTSANLESDSLQAIRGCMRRLRAEQKVHSLVLLKVMHDCVQLCNDRGAVLATYPAEKLAFSAVCPDDRRFFGLVTMQATNDHRMYRHGEEEQGLRTSCHVFIVDPELCHHKIHQGIARRFGFDCTPDPDTGGCLEFPPTSQPLLQFVSVLYRDMGESIEGMRARAFLDGDLDAQQNNSTSSNSDSGIGNFLPEEKNNRVLLVDLGANPSRHVPTGPWENPMGSFCQGAIGGLPPPPPPPPISHRNGFRHEQFVDHPQPHPHSHGDPPLLSGRHLNSSSRLDVSGLPSRSHYSAHGKKAAGASGQRWLPVHVLRDWRQGHSSDQESYAESTDGWSSANCSTLPPPMSKIPADRYRAAGEIASQPHRLHAQKDEWAKKLFGEKNLREHSQQSNNVRRAKDSEKKGGRFKGITMGFPQRSSGRRSFGRSKRLSMARSLDDLESAAVSDGELNSVDLKGCGSDNSLNSNASLPSVQCHRRHTERRVASWAVCFERLLQDPVGVRYFSEFLKKEFSEENILFWQACEYFSHVPETDKKQLSQRAREIYNSFLSSKATTPVNIDSQAQLADDILNAPRPDMFKEQQLQIFNLMKFDSYTRFLKSYLYQECMLAEVEGRPLPDPYQVPSSPTSKHSTGSDRSNLSTPKKVQDDKKSKSGRSLNEDSRDEHGDKKKSIFFSWSRNRSFGKGSKKRDLADYSFTGSNGRRESQGSLSSGASLELATSGSGKNEGEVSRNSMSLSERAVRHCNINLPDGSCCSVPIRPGVSIREVLLGLCEKLGINLAAVDLFLVGGEKPLVLDQDCMTLCSRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLNLGDLVATISGEKEPLDLGLPISNLDGLRVVLEAGDHVSGKDKQKLVSSKSHGPPLSTRSQSATGEDKTAGKASAVKTRAQVEKRKQKKLNIDEAEEFFELISKAQSNRADDQRGLLNKSDLVLPDFLRLDTEADGNNLSSTPISHKTRPRSKENGSALSASHQTQSLDSAGEGKAPRRALMPPNSALRNTSSRIPPPFSSSLSPIPHSQMGGSSGITDWRSDGGVQALEEEESGMDLTFVAEGDITSPNSSLLHQSPIPLPPSPERSHLLKEDTCQDSWARSGGLMTKTVVSNRNRFSSVKNERRQRDRTSVSKYSHRRAPSAPPPPPPHPPPAPPSAPPRVRGAGKRSPLHVRGALDVDGIHCGDLGGDNRESEGLERVVGDLKTSKGRMRTGVYQTSDQAELLPTKKRIDPVAKKSIKPSVKTSSKKSNHSKNNATFV

Target ORF information:

RefSeq Version	NM_214724.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulator of G protein signaling 12b (rgs12b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214724.1

ORF Insert Sequence:

ATGTTCAGGC AATCAGAACA AGGCAGGAGG CGTGTAAATG CCCAGCCATC TGCTCGCATT 
AGAGGAGTGG AGGTTGCAAG GGGGAGAACA GGTTATGGCT TCACACTCTC TGGTCAGTCT 
CCATGTGTTC TGAATTGCAT CCTCAAGGGT AGTCCAGCTG ATTATGTGGG CCTTCGCTCT 
GGAGATCAGA TCCTGAGTGT GAATGACATT AATGTGTCCA AAGCTTCTCA TGAGGATGTT 
GTTAAGCTGA TTGGCCGCTG CACAGGTGTC CTACACTTAG TCATTGCCGA AGGCAGTCAT 
AAAAACGGCC ATGTTGATTC GTGTTCAAGT GATGAAGAAC TGGAGTTCCA TGACCACAAG 
AGCCACTGGC TGAGGCCAAA GATGGACTCA AAGACACTAG GCATAAACCG TGCAGAACGT 
GTTGTAGCAG AGATGCAGTC TGGTGGGATC TTCAACATGA TCTTTGAAAA TTCAAGCCAC 
TCATCCAGCA GCTCAGAAAG AGCAGTTGCT TGTGCCTCTG CTTCCAAGCC AAGGGCTGTC 
TCAGAGCCAG AATTCCCTTA TGTGAGCGTC AACACCCAAA AAAGTGATCC CAACTTGCTC 
ACTGAGGAAG AGATGGCCAA AGTGCTCAAT GACGACTCTG TGTTTGTTGA TATGTTTGAA 
CCGCAAGATG AATTTGCCCT GTTGCAAACT GCAGACTCTG CAGGAATACT CAATGTTGGG 
ATGATTGTGG GTTACTTGGG ATCTATTGAA TTAGCTTCAA CAAGTGCCAA TTTAGAAAGT 
GACAGCCTGC AGGCAATCCG TGGCTGTATG CGACGTCTAC GTGCCGAACA AAAAGTGCAC 
TCGTTGGTTT TGCTTAAAGT CATGCATGAT TGTGTGCAAC TATGCAATGA CCGAGGTGCT 
GTTCTAGCAA CTTACCCCGC TGAGAAGTTA GCCTTCAGTG CTGTGTGTCC AGACGACCGC 
CGCTTTTTTG GCCTTGTCAC CATGCAAGCA ACAAATGACC ACCGCATGTA TAGGCATGGT 
GAGGAGGAGC AAGGCTTACG GACATCTTGT CATGTCTTCA TTGTAGACCC GGAACTCTGT 
CATCATAAAA TCCACCAAGG TATTGCCCGA CGCTTTGGCT TTGACTGCAC TCCAGACCCA 
GATACTGGTG GCTGCTTGGA GTTCCCACCG ACTTCTCAGC CACTTCTACA GTTTGTGTCT 
GTTCTGTATC GTGACATGGG AGAATCCATA GAGGGTATGC GAGCTCGAGC TTTCCTTGAT 
GGTGACTTAG ATGCTCAGCA GAACAACAGC ACAAGCAGCA ACAGTGACAG CGGCATTGGG 
AACTTCTTGC CAGAGGAAAA GAACAACCGA GTCCTCTTGG TGGACTTGGG TGCAAATCCT 
AGTCGACATG TTCCTACAGG ACCTTGGGAA AACCCTATGG GATCTTTTTG CCAGGGTGCC 
ATAGGAGGTC TTCCGCCACC ACCTCCTCCG CCACCGATCT CACATCGTAA CGGATTTCGG 
CATGAGCAGT TTGTGGATCA CCCTCAGCCC CATCCACATT CCCATGGAGA CCCACCTCTG 
CTTTCTGGCA GGCATCTAAA CTCCTCCTCA CGGCTGGATG TGTCAGGCCT TCCTTCGCGC 
TCACACTATT CTGCTCATGG CAAGAAAGCG GCAGGGGCAT CAGGCCAGCG CTGGCTGCCA 
GTTCACGTCC TGAGGGATTG GCGTCAGGGA CACAGCAGTG ATCAGGAGTC TTATGCAGAG 
TCCACAGACG GATGGTCCAG TGCTAACTGT AGCACCCTGC CACCACCGAT GAGCAAGATT 
CCAGCTGACC GGTACCGTGC TGCAGGCGAA ATCGCCTCAC AGCCTCACCG GCTTCATGCA 
CAGAAGGATG AGTGGGCTAA GAAGCTGTTT GGGGAGAAGA ACCTACGAGA GCATTCACAA 
CAATCGAACA ATGTCAGAAG AGCTAAAGAT TCAGAAAAAA AGGGTGGAAG GTTCAAAGGG 
ATCACTATGG GCTTTCCTCA GCGTTCCTCT GGCCGACGAT CTTTTGGACG TTCCAAGCGT 
CTTAGCATGG CTCGCTCACT TGATGACCTT GAGTCTGCAG CTGTCTCTGA TGGTGAGCTT 
AACAGTGTGG ACCTGAAAGG CTGCGGCAGT GATAACAGTC TGAACAGCAA TGCCAGCCTA 
CCTAGCGTGC AATGCCATAG ACGGCACACT GAACGGCGCG TTGCATCGTG GGCCGTCTGC 
TTTGAGCGCC TGCTACAGGA TCCCGTCGGT GTCCGATATT TCTCGGAGTT TCTAAAGAAG 
GAGTTTAGCG AAGAGAATAT TTTATTTTGG CAAGCTTGTG AATATTTCAG CCATGTACCT 
GAAACAGACA AGAAACAGCT GTCCCAGCGG GCCAGAGAGA TCTACAACAG CTTCCTGTCT 
AGCAAAGCTA CCACACCCGT CAACATTGAT AGTCAAGCTC AGCTGGCTGA CGACATCCTC 
AACGCACCGA GGCCAGACAT GTTTAAAGAG CAACAGCTTC AGATCTTCAA CCTGATGAAG 
TTTGACAGTT ACACACGCTT CCTAAAGTCT TACCTGTATC AGGAATGTAT GCTTGCAGAG 
GTGGAGGGCC GTCCACTTCC AGACCCCTAC CAGGTGCCCA GTAGCCCCAC ATCCAAACAT 
AGCACAGGTT CCGACCGCTC CAACCTCTCT ACACCTAAAA AGGTGCAGGA CGACAAGAAG 
AGCAAATCGG GAAGGTCACT GAATGAAGAC AGCAGAGATG AACATGGCGA TAAAAAAAAA 
AGTATTTTCT TCTCTTGGTC CAGAAATAGA AGTTTTGGAA AAGGGTCGAA AAAAAGAGAC 
CTCGCAGATT ACAGCTTTAC AGGTAGTAAC GGAAGGAGAG AATCACAAGG CTCTTTGTCG 
TCTGGTGCGA GTTTGGAACT TGCCACTTCC GGTTCTGGAA AAAATGAGGG TGAGGTGTCG 
CGTAACTCCA TGTCGTTGTC TGAGCGTGCC GTGCGTCACT GTAACATTAA CCTACCGGAT 
GGCTCATGCT GCTCAGTACC CATCAGGCCC GGCGTCTCCA TCAGAGAGGT GCTCTTAGGC 
TTGTGTGAGA AACTAGGCAT TAATCTGGCT GCTGTGGACC TGTTCCTAGT GGGAGGAGAA 
AAGCCTTTAG TGTTGGACCA GGACTGCATG ACACTGTGCT CCAGGGATCT GCGGCTGGAG 
AAACGTACGC TATTCCGACT GGATCTGGTG CCTATAAATC GTTCTGTTGG ACTGAAAGCA 
AAACCCACTA AGCCGGTTAC AGAAGTGCTG AGACCAGTGG TGGCTAAATA TGGTTTAAAC 
TTAGGAGACC TGGTGGCCAC GATAAGTGGA GAGAAAGAGC CGCTGGATCT TGGGCTTCCT 
ATATCTAATC TAGATGGCCT GAGGGTGGTT CTTGAAGCAG GTGACCACGT TTCTGGGAAA 
GATAAACAAA AGCTGGTCTC ATCGAAAAGT CACGGTCCAC CTCTGTCTAC ACGAAGCCAA 
TCTGCCACAG GGGAGGACAA AACGGCAGGA AAGGCTTCGG CTGTGAAAAC CAGGGCCCAG 
GTGGAAAAGC GAAAACAGAA AAAGCTTAAT ATAGATGAAG CCGAAGAATT CTTCGAGCTC 
ATATCCAAAG CACAGAGCAA CAGAGCTGAC GACCAGCGGG GTTTGCTGAA CAAATCAGAC 
CTTGTCCTGC CCGACTTCCT GCGCCTCGAC ACGGAAGCAG ACGGCAACAA CCTCAGCTCC 
ACACCCATCT CCCACAAGAC TCGACCACGA TCCAAGGAGA ATGGCTCTGC CCTCAGTGCC 
AGTCACCAAA CGCAGAGCTT AGATTCAGCG GGGGAGGGCA AAGCACCCAG ACGAGCACTA 
ATGCCACCCA ACAGTGCCCT CAGAAACACA TCCAGCCGAA TTCCGCCACC GTTCAGTTCC 
TCGCTCTCCC CAATCCCACA TTCGCAAATG GGGGGCTCGT CCGGGATCAC AGACTGGAGG 
AGTGACGGAG GGGTCCAGGC ACTGGAGGAA GAGGAGAGCG GCATGGACCT GACTTTTGTT 
GCCGAAGGCG ACATCACAAG CCCTAACAGC TCTCTCCTTC ACCAGTCCCC CATTCCTCTC 
CCTCCTTCCC CAGAGCGATC ACACCTCCTG AAGGAGGACA CTTGCCAGGA CAGCTGGGCT 
CGATCAGGCG GACTTATGAC CAAGACAGTG GTATCAAACA GAAACCGCTT CTCTTCAGTG 
AAAAATGAAA GACGGCAGAG AGACAGGACC AGTGTTTCCA AATATTCCCA CAGGAGGGCA 
CCCTCCGCCC CACCTCCTCC ACCTCCACAT CCACCTCCCG CTCCTCCATC AGCTCCACCC 
AGGGTCAGAG GAGCAGGCAA GCGCTCTCCT CTTCATGTGC GAGGGGCTCT TGACGTTGAT 
GGGATCCATT GTGGTGACCT TGGTGGTGAT AATAGAGAAT CCGAGGGCTT GGAGAGGGTG 
GTTGGTGATC TGAAAACCAG CAAAGGAAGA ATGAGAACAG GAGTTTATCA GACCTCAGAC 
CAAGCTGAAC TGCTGCCTAC GAAAAAGCGC ATTGATCCAG TGGCAAAAAA ATCAATCAAG 
CCTTCAGTTA AAACGTCAAG CAAGAAGAGC AACCACAGCA AAAACAATGC CACTTTTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rgs12b ( XM_021481388.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337063.1 Danio rerio regulator of G protein signaling 12b (rgs12b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40338
Clone ID Related Accession (Same CDS sequence)	XM_021481388.1
Accession Version	XM_021481388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	regulator of G-protein signaling 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGGC AATCAGAACA AGGCAGGAGG CGTGTAAATG CCCAGCCATC TGCTCGCATT 
AGAGGAGTGG AGGTTGCAAG GGGGAGAACA GGCTATGGCT TCACACTCTC TGGTCAGTCT 
CCATGTGTTC TGAATTGCAT CCTTAAGGGT AGTCCAGCTG ATTATGTGGG CCTTCGCTCT 
GGAGATCAGA TCCTGAGTGT GAATGACATT AATGTGTCCA AAGCTTCTCA TGAGGATGTT 
GTTAAGCTGA TTGGCCGCTG CACAGGTGTC CTACACTTAG TCATTGCCGA AGGCAGTCAT 
AAAAACGGCC ACGTTGATTC GTGTTCAAGT GATGAAGAAC TGGAGTTCCA TGACCACAAG 
AGCCACTGGC TGAGGCCAAA GATGGACTCA AAGACACTAG GCATAAACCG TGCAGAACGT 
GTTGTAGCAG AGATGCAGTC TGGTGGGATC TTCAACATGA TCTTTGAAAA TTCGAGCCAC 
TCATCCAGCA GCTCAGAAAG AGCAGTTGCT TGTGCCTCTG CTTCCAAACC AAGGGCTGTC 
TCAGAGCCAG AATTCCCTTA TGTGAGCGTC AACACCCAAA AAAGTGATCC CAACTTGCTC 
ACTGAGGAAG AGATGGCCAA AGTGCTCAAT GACGACTCTG TGTTTGTTGA TATGTTTGAA 
CCGCAAGATG AATTTGCCCT GTTGCAAACT GCAGACTCTG CAGGAATACT CAATGTTGGG 
ATGATTGTGG GTTACTTGGG ATCTATTGAA TTAGCTTCAA CAAGTGCCAA TTTAGAAAGT 
GACAGCCTGC AGGCAATACG TGGCTGTATG CGACGTCTAC GTGCCGAACA AAAAGTGCAC 
TCGTTGGTTT TGCTTAAAGT CATGCATGAT TGTGTGCAAC TATGCAATGA CCGAGGTGCT 
GTTCTAGCAA CTTACCCTGC AGAGAAGTTA GCCTTCAGTG CTGTGTGTCC AGACGACCGC 
CGCTTTTTTG GCCTTGTCAC CATGCAAGCA ACAAATGACC ACCGCATGTA TAGGCATGGT 
GAGGAGGAGC AAGGCTTACG GACATCTTGT CATGTCTTCA TTGTAGACCC GGAACTCTGT 
CATCATAAAA TCCACCAGGG TATTGCCCGA CGCTTTGGCT TTGACTGCAC TCCAGACCCA 
GATACTGGTG GCTGCTTGGA GTTCCCACCG ACTTCTCAGC CACTTCTACA GTTTGTGTCT 
GTTCTGTATC GTGACATGGG AGAATCCATA GAGGGTATGC GAGCTCGAGC TTTCCTTGAT 
GGTGACTTAG ATGCTCAGCA GAACAACAGC ACAAGCAGCA ACAGTGACAG CGGCATTGGG 
AACTTCTTGC CAGAGGAAAA GAACAACCGA GTCCTCTTGG TGGACTTGGG TGCAAATCCT 
AGTCGACATG TTCCTACAGG ACCTTGGGAA AACCCTATGG GATCTTTTTG CCAGGGTGCC 
ATAGGAGGTC TTCCGCCACC ACCTCCTCCG CCACCGATCT CACATCGTAA CGGATTTCGG 
CATGAGCAGT TTGTGGATCA CCCTCAGCCC CATCCACATT CCCATGGAGA CCCACCTCTG 
CTTTCTGGCA GGCATCTAAA CTCCTCCTCA CGGCTGGATG TGTCAGGCCT TCCTTCGCGC 
TCACACTATT CTGCTCATGG CAAGAAAGCG GCAGGGGCAT CAGGCCAGCG CTGGCTGCCA 
GTTCACGTCC TGAGGGATTG GCGTCAGGGA CACAGCAGTG ATCAGGAGTC TTATGCAGAG 
TCCACAGACG GATGGTCCAG TGCTAACTGT AGCACCCTGC CACCACCGAT GAGCAAGATT 
CCAGCTGACC GGTACCGTGC TGCAGGCGAA ATCGCCTCAC AGCCTCACCG GCTTCATGCA 
CAGAAGGATG AGTGGGCTAA GAAGCTGTTT GGGGAGAAGA ACCTACGAGA GCATTCACAA 
CAATCGAACA ATGTCAGAAG AGCTAAAGAT TCAGAAAAAA AGGGTGGAAG GTTCAAAGGG 
ATCACTATGG GCTTTCCTCA GCGTTCCTCT GGCCGACGAT CTTTTGGACG TTCCAAGCGT 
CTTAGCATGG CTCGCTCACT TGATGACCTT GAGTCTGCAG CTGTCTCTGA TGGTGAGCTT 
AACAGTGTGG ACCTGAAAGG CTGCGGCAGT GATAACAGTC TGAACAGCAA TGCCAGCCTA 
CCTAGCGTGC AATGCCATAG ACGGCACACT GAACGGCGCG TTGCATCGTG GGCCGTCTGC 
TTTGAGCGCC TGCTACAGGA TCCCGTCGGT GTCCGATATT TCTCGGAGTT TCTAAAGAAG 
GAGTTTAGCG AAGAGAATAT TTTATTTTGG CAAGCTTGTG AATATTTCAG CCATGTACCT 
GAAACAGACA AGAAACAGCT GTCCCAGCGG GCCAGAGAGA TCTACAACAG CTTCCTGTCT 
AGTAAAGCTA CCACACCCGT CAACATTGAT AGTCAAGCTC AGCTGGCTGA CGACATCCTC 
AACGCACCGA GGCCAGACAT GTTTAAAGAG CAACAGCTTC AGATCTTCAA CCTGATGAAG 
TTTGACAGTT ACACACGCTT CCTAAAGTCT TACCTGTATC AGGAATGTAT GCTTGCAGAG 
GTGGAGGGCC GTCCACTTCC AGACCCCTAC CAGGTGCCCA GTAGCCCCAC ATCCAAACAT 
AGCACAGGTT CCGACCGCTC CAACCTCTCT ACACCTAAAA AGGTGCAGGA CGACAAGAAG 
AGCAAATCGG GAAGGTCACT GAATGAAGAC AGCAGAGATG AACATGGCGA TAAAAAAAAA 
AGTATTTTCT TCTCTTGGTC CAGAAATAGA AGTTTTGGAA AAGGGTCGAA AAAAAGAGAC 
CTCGCAGATT ACAGCTTTAC AGGTAGTAAC GGAAGGAGAG AATCACAAGG CTCTTTGTCG 
TCTGGTGCGA GTTTGGAACT TGCCACTTCC GGTTCTGGAA AAAATGAGGG TGAGGTGTCG 
CGTAACTCCA TGTCGTTGTC TGAGCGTGCC GTGCGTCACT GTAACATTAA CCTACCGGAT 
GGCTCATGCT GCTCAGTACC CATCAGGCCC GGCGTCTCCA TCAGAGAGGT GCTCTTAGGC 
TTGTGTGAGA AACTAGGCAT TAATCTGGCT GCTGTGGACC TGTTCCTAGT AGGAGGAGAA 
AAGCCTTTAG TGTTGGACCA GGACTGCATG ACACTGTGCT CCAGGGATCT GCGGCTGGAG 
AAACGTACGC TATTCCGACT GGATCTGGTG CCTATAAATC GTTCTGTTGG ACTGAAAGCA 
AAACCCACTA AGCCGGTTAC AGAAGTGCTG AGACCAGTGG TGGCTAAATA TGGTTTAAAC 
TTAGGAGACC TGGTGGCCAC GATAAGTGGA GAGAAAGAGC CGCTGGATCT TGGGCTTCCT 
ATATCTAATC TAGATGGCCT GAGGGTGGTT CTTGAAGCAG GTGACCACGT TTCTGGGAAA 
GATAAACAAA AGCTGGTCTC ATCGAAAAGT CACGGTCCAC CTCTGTCTAC ACGAAGCCAA 
TCTGCCACAG GGGAGGACAA AACGGCAGGA AAGGCTTCGG CTGTGAAAAC CAGGGCCCAG 
GTGGAAAAGC GAAAACAGAA AAAGCTTAAT ATAGATGAAG CTGAAGAATT CTTCGAGCTC 
ATATCCAAAG CACAGAGCAA CAGAGCTGAC GACCAGCGGG GTTTGCTGAA CAAATCAGAC 
CTTGTCCTGC CCGACTTCCT GCGCCTCGAC ACGGAAGCAG ACGGCAACAA CCTCAGCTCC 
ACACCCATCT CCCACAAGAC TCGACCACGA TCCAAGGAGA ATGGCTCTGC CCTCAGTGCC 
AGTCACCAAA CGCAGAGCTT AGATTCAGCG GGGGAGGGCA AAGCACCCAG ACGAGCACTA 
ATGCCACCCA ACAGTGCCCT CAGAAACACA TCCAGCCGAA TTCCGCCACC GTTCAGTTCC 
TCGCTCTCCC CAATCCCACA TTCGCAAATG GGGGGCTCGT CCGGGATCAC AGACTGGAGG 
AGTGACGGAG GGGTCCAGGC ACTGGAGGAA GAGGAGAGCG GCATGGACCT GACTTTTGTT 
GCCGAAGGCG ACATCACAAG CCCTAACAGC TCTCTCCTTC ACCAGTCCCC CATTCCTCTC 
CCTCCTTCCC CAGAGCGATC ACACCTCCTG AAGGAGGACA CTTGCCAGGA CAGCTGGGCT 
CGATCAGGTA CCTCTCCTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337063.1
CDS	244..4407
Translation	MFRQSEQGRRRVNAQPSARIRGVEVARGRTGYGFTLSGQSPCVLNCILKGSPADYVGLRSGDQILSVNDINVSKASHEDVVKLIGRCTGVLHLVIAEGSHKNGHVDSCSSDEELEFHDHKSHWLRPKMDSKTLGINRAERVVAEMQSGGIFNMIFENSSHSSSSSERAVACASASKPRAVSEPEFPYVSVNTQKSDPNLLTEEEMAKVLNDDSVFVDMFEPQDEFALLQTADSAGILNVGMIVGYLGSIELASTSANLESDSLQAIRGCMRRLRAEQKVHSLVLLKVMHDCVQLCNDRGAVLATYPAEKLAFSAVCPDDRRFFGLVTMQATNDHRMYRHGEEEQGLRTSCHVFIVDPELCHHKIHQGIARRFGFDCTPDPDTGGCLEFPPTSQPLLQFVSVLYRDMGESIEGMRARAFLDGDLDAQQNNSTSSNSDSGIGNFLPEEKNNRVLLVDLGANPSRHVPTGPWENPMGSFCQGAIGGLPPPPPPPPISHRNGFRHEQFVDHPQPHPHSHGDPPLLSGRHLNSSSRLDVSGLPSRSHYSAHGKKAAGASGQRWLPVHVLRDWRQGHSSDQESYAESTDGWSSANCSTLPPPMSKIPADRYRAAGEIASQPHRLHAQKDEWAKKLFGEKNLREHSQQSNNVRRAKDSEKKGGRFKGITMGFPQRSSGRRSFGRSKRLSMARSLDDLESAAVSDGELNSVDLKGCGSDNSLNSNASLPSVQCHRRHTERRVASWAVCFERLLQDPVGVRYFSEFLKKEFSEENILFWQACEYFSHVPETDKKQLSQRAREIYNSFLSSKATTPVNIDSQAQLADDILNAPRPDMFKEQQLQIFNLMKFDSYTRFLKSYLYQECMLAEVEGRPLPDPYQVPSSPTSKHSTGSDRSNLSTPKKVQDDKKSKSGRSLNEDSRDEHGDKKKSIFFSWSRNRSFGKGSKKRDLADYSFTGSNGRRESQGSLSSGASLELATSGSGKNEGEVSRNSMSLSERAVRHCNINLPDGSCCSVPIRPGVSIREVLLGLCEKLGINLAAVDLFLVGGEKPLVLDQDCMTLCSRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVAKYGLNLGDLVATISGEKEPLDLGLPISNLDGLRVVLEAGDHVSGKDKQKLVSSKSHGPPLSTRSQSATGEDKTAGKASAVKTRAQVEKRKQKKLNIDEAEEFFELISKAQSNRADDQRGLLNKSDLVLPDFLRLDTEADGNNLSSTPISHKTRPRSKENGSALSASHQTQSLDSAGEGKAPRRALMPPNSALRNTSSRIPPPFSSSLSPIPHSQMGGSSGITDWRSDGGVQALEEEESGMDLTFVAEGDITSPNSSLLHQSPIPLPPSPERSHLLKEDTCQDSWARSGTSPV

Target ORF information:

RefSeq Version	XM_021481388.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulator of G protein signaling 12b (rgs12b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481388.1

ORF Insert Sequence:

ATGTTCAGGC AATCAGAACA AGGCAGGAGG CGTGTAAATG CCCAGCCATC TGCTCGCATT 
AGAGGAGTGG AGGTTGCAAG GGGGAGAACA GGCTATGGCT TCACACTCTC TGGTCAGTCT 
CCATGTGTTC TGAATTGCAT CCTTAAGGGT AGTCCAGCTG ATTATGTGGG CCTTCGCTCT 
GGAGATCAGA TCCTGAGTGT GAATGACATT AATGTGTCCA AAGCTTCTCA TGAGGATGTT 
GTTAAGCTGA TTGGCCGCTG CACAGGTGTC CTACACTTAG TCATTGCCGA AGGCAGTCAT 
AAAAACGGCC ACGTTGATTC GTGTTCAAGT GATGAAGAAC TGGAGTTCCA TGACCACAAG 
AGCCACTGGC TGAGGCCAAA GATGGACTCA AAGACACTAG GCATAAACCG TGCAGAACGT 
GTTGTAGCAG AGATGCAGTC TGGTGGGATC TTCAACATGA TCTTTGAAAA TTCGAGCCAC 
TCATCCAGCA GCTCAGAAAG AGCAGTTGCT TGTGCCTCTG CTTCCAAACC AAGGGCTGTC 
TCAGAGCCAG AATTCCCTTA TGTGAGCGTC AACACCCAAA AAAGTGATCC CAACTTGCTC 
ACTGAGGAAG AGATGGCCAA AGTGCTCAAT GACGACTCTG TGTTTGTTGA TATGTTTGAA 
CCGCAAGATG AATTTGCCCT GTTGCAAACT GCAGACTCTG CAGGAATACT CAATGTTGGG 
ATGATTGTGG GTTACTTGGG ATCTATTGAA TTAGCTTCAA CAAGTGCCAA TTTAGAAAGT 
GACAGCCTGC AGGCAATACG TGGCTGTATG CGACGTCTAC GTGCCGAACA AAAAGTGCAC 
TCGTTGGTTT TGCTTAAAGT CATGCATGAT TGTGTGCAAC TATGCAATGA CCGAGGTGCT 
GTTCTAGCAA CTTACCCTGC AGAGAAGTTA GCCTTCAGTG CTGTGTGTCC AGACGACCGC 
CGCTTTTTTG GCCTTGTCAC CATGCAAGCA ACAAATGACC ACCGCATGTA TAGGCATGGT 
GAGGAGGAGC AAGGCTTACG GACATCTTGT CATGTCTTCA TTGTAGACCC GGAACTCTGT 
CATCATAAAA TCCACCAGGG TATTGCCCGA CGCTTTGGCT TTGACTGCAC TCCAGACCCA 
GATACTGGTG GCTGCTTGGA GTTCCCACCG ACTTCTCAGC CACTTCTACA GTTTGTGTCT 
GTTCTGTATC GTGACATGGG AGAATCCATA GAGGGTATGC GAGCTCGAGC TTTCCTTGAT 
GGTGACTTAG ATGCTCAGCA GAACAACAGC ACAAGCAGCA ACAGTGACAG CGGCATTGGG 
AACTTCTTGC CAGAGGAAAA GAACAACCGA GTCCTCTTGG TGGACTTGGG TGCAAATCCT 
AGTCGACATG TTCCTACAGG ACCTTGGGAA AACCCTATGG GATCTTTTTG CCAGGGTGCC 
ATAGGAGGTC TTCCGCCACC ACCTCCTCCG CCACCGATCT CACATCGTAA CGGATTTCGG 
CATGAGCAGT TTGTGGATCA CCCTCAGCCC CATCCACATT CCCATGGAGA CCCACCTCTG 
CTTTCTGGCA GGCATCTAAA CTCCTCCTCA CGGCTGGATG TGTCAGGCCT TCCTTCGCGC 
TCACACTATT CTGCTCATGG CAAGAAAGCG GCAGGGGCAT CAGGCCAGCG CTGGCTGCCA 
GTTCACGTCC TGAGGGATTG GCGTCAGGGA CACAGCAGTG ATCAGGAGTC TTATGCAGAG 
TCCACAGACG GATGGTCCAG TGCTAACTGT AGCACCCTGC CACCACCGAT GAGCAAGATT 
CCAGCTGACC GGTACCGTGC TGCAGGCGAA ATCGCCTCAC AGCCTCACCG GCTTCATGCA 
CAGAAGGATG AGTGGGCTAA GAAGCTGTTT GGGGAGAAGA ACCTACGAGA GCATTCACAA 
CAATCGAACA ATGTCAGAAG AGCTAAAGAT TCAGAAAAAA AGGGTGGAAG GTTCAAAGGG 
ATCACTATGG GCTTTCCTCA GCGTTCCTCT GGCCGACGAT CTTTTGGACG TTCCAAGCGT 
CTTAGCATGG CTCGCTCACT TGATGACCTT GAGTCTGCAG CTGTCTCTGA TGGTGAGCTT 
AACAGTGTGG ACCTGAAAGG CTGCGGCAGT GATAACAGTC TGAACAGCAA TGCCAGCCTA 
CCTAGCGTGC AATGCCATAG ACGGCACACT GAACGGCGCG TTGCATCGTG GGCCGTCTGC 
TTTGAGCGCC TGCTACAGGA TCCCGTCGGT GTCCGATATT TCTCGGAGTT TCTAAAGAAG 
GAGTTTAGCG AAGAGAATAT TTTATTTTGG CAAGCTTGTG AATATTTCAG CCATGTACCT 
GAAACAGACA AGAAACAGCT GTCCCAGCGG GCCAGAGAGA TCTACAACAG CTTCCTGTCT 
AGTAAAGCTA CCACACCCGT CAACATTGAT AGTCAAGCTC AGCTGGCTGA CGACATCCTC 
AACGCACCGA GGCCAGACAT GTTTAAAGAG CAACAGCTTC AGATCTTCAA CCTGATGAAG 
TTTGACAGTT ACACACGCTT CCTAAAGTCT TACCTGTATC AGGAATGTAT GCTTGCAGAG 
GTGGAGGGCC GTCCACTTCC AGACCCCTAC CAGGTGCCCA GTAGCCCCAC ATCCAAACAT 
AGCACAGGTT CCGACCGCTC CAACCTCTCT ACACCTAAAA AGGTGCAGGA CGACAAGAAG 
AGCAAATCGG GAAGGTCACT GAATGAAGAC AGCAGAGATG AACATGGCGA TAAAAAAAAA 
AGTATTTTCT TCTCTTGGTC CAGAAATAGA AGTTTTGGAA AAGGGTCGAA AAAAAGAGAC 
CTCGCAGATT ACAGCTTTAC AGGTAGTAAC GGAAGGAGAG AATCACAAGG CTCTTTGTCG 
TCTGGTGCGA GTTTGGAACT TGCCACTTCC GGTTCTGGAA AAAATGAGGG TGAGGTGTCG 
CGTAACTCCA TGTCGTTGTC TGAGCGTGCC GTGCGTCACT GTAACATTAA CCTACCGGAT 
GGCTCATGCT GCTCAGTACC CATCAGGCCC GGCGTCTCCA TCAGAGAGGT GCTCTTAGGC 
TTGTGTGAGA AACTAGGCAT TAATCTGGCT GCTGTGGACC TGTTCCTAGT AGGAGGAGAA 
AAGCCTTTAG TGTTGGACCA GGACTGCATG ACACTGTGCT CCAGGGATCT GCGGCTGGAG 
AAACGTACGC TATTCCGACT GGATCTGGTG CCTATAAATC GTTCTGTTGG ACTGAAAGCA 
AAACCCACTA AGCCGGTTAC AGAAGTGCTG AGACCAGTGG TGGCTAAATA TGGTTTAAAC 
TTAGGAGACC TGGTGGCCAC GATAAGTGGA GAGAAAGAGC CGCTGGATCT TGGGCTTCCT 
ATATCTAATC TAGATGGCCT GAGGGTGGTT CTTGAAGCAG GTGACCACGT TTCTGGGAAA 
GATAAACAAA AGCTGGTCTC ATCGAAAAGT CACGGTCCAC CTCTGTCTAC ACGAAGCCAA 
TCTGCCACAG GGGAGGACAA AACGGCAGGA AAGGCTTCGG CTGTGAAAAC CAGGGCCCAG 
GTGGAAAAGC GAAAACAGAA AAAGCTTAAT ATAGATGAAG CTGAAGAATT CTTCGAGCTC 
ATATCCAAAG CACAGAGCAA CAGAGCTGAC GACCAGCGGG GTTTGCTGAA CAAATCAGAC 
CTTGTCCTGC CCGACTTCCT GCGCCTCGAC ACGGAAGCAG ACGGCAACAA CCTCAGCTCC 
ACACCCATCT CCCACAAGAC TCGACCACGA TCCAAGGAGA ATGGCTCTGC CCTCAGTGCC 
AGTCACCAAA CGCAGAGCTT AGATTCAGCG GGGGAGGGCA AAGCACCCAG ACGAGCACTA 
ATGCCACCCA ACAGTGCCCT CAGAAACACA TCCAGCCGAA TTCCGCCACC GTTCAGTTCC 
TCGCTCTCCC CAATCCCACA TTCGCAAATG GGGGGCTCGT CCGGGATCAC AGACTGGAGG 
AGTGACGGAG GGGTCCAGGC ACTGGAGGAA GAGGAGAGCG GCATGGACCT GACTTTTGTT 
GCCGAAGGCG ACATCACAAG CCCTAACAGC TCTCTCCTTC ACCAGTCCCC CATTCCTCTC 
CCTCCTTCCC CAGAGCGATC ACACCTCCTG AAGGAGGACA CTTGCCAGGA CAGCTGGGCT 
CGATCAGGTA CCTCTCCTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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rgs12b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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