RGS12 cDNA ORF clone, Canis lupus familiaris(dog)

The following RGS12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RGS12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe35796 XM_014112534.2
Latest version!
Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe35797 XM_014112536.2
Latest version!
Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe35798 XM_014112538.2
Latest version!
Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe35799 XM_014112539.2
Latest version!
Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe35800 XM_014112540.2
Latest version!
Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe04433 XM_014112535.2
Latest version!
Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe04434 XM_014112537.2
Latest version!
Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe04433 XM_014112535.1 Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe35799 XM_014112539.1 Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe35800 XM_014112540.1 Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe35796 XM_014112534.1 Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe35797 XM_014112536.1 Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe04434 XM_014112537.1 Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe35798 XM_014112538.1 Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe35796
    Clone ID Related Accession (Same CDS sequence) XM_014112534.2 , XM_014112534.1
    Accession Version XM_014112534.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 12 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112534.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCAG CTGAGTGGAA AATCGAAATC AGGAAGATCC
    CTGAACGAAG AGCTGGGGGA TGAGGATAGT GAGAAGAAGC GGAAAGGAGC CTTCTTCTCT
    TGGTCAAGGA CCAGGAGCAC TGGGCGGTCC CAGAAGAAGA AGGAGCATGG TGACCACACA
    AACGAGCCCC TGCATGCCAA TGGAGGCCTG GGCCGCCGCG AGTCGCAGGG CTCCGTGTCC
    TCTGCGGGGA GTCTGGACCT GTCGGATGCC TGTAGGACCT TGATGCCAGA GCGGGACAAG
    GCCGCCAAGC ACTGCTGCAT CCAGCTCCCC GATGGGACGT CCTGCATGGT GGTCGTCAAG
    GCAGGCTTAT CCATCAAGGA GGTCCTGGCT GGGCTCTGCG AGCGACATGG CATCAACGGG
    GCTGCCGTAG ACCTCTTCCT GGTGGGCGGG GACAAGCCTC TGGTGCTGCA TCAAGACAGT
    AGCATCTTGG AGTCTCGGGA CCTGCGGCTA GAAAAGCGGA CTTTGTTTCG GCTGGATCTG
    GTTCCAATTA ACCGGTCAGT GGGACTGAAG GCCAAGCCGA CCAAGCCTGT CACAGAAGTA
    CTGCGGCCCG TGGTAGCCAA GTATGGCCTG CAGCTGAACG AGCTGGTGGC CAGGCTGAGT
    GGAGAGAAGG AGCCCCTGGA CCTTGGTGCC CCCATATCAA GTCTGGATGG GCAGAGGGTC
    ATCTTGGAGG AGAAGGAGCC TTCCAGGGGA AAAGGATCCA CAGATAAACA GAAAAGTGCA
    CCAGCCAAAC AGAATATAGC TGTAAATTCC AGTTCCAGAA ATCACTCGGC TACGGGAGAG
    GAGAGAACGC TAGGCAAGTC TAATTCTATT AAAATAAAAG GAGAAAATGG AAAAAATGCT
    AGGGATCCCC GGCTTTCAAA GAGAGAAGAA TCTATTGCAA AGATTGGGAA AAAAAAATAT
    CAGAAAATTA ATTTGGACGA AGCAGAGGAG TTTTTTGAGC TCATTTCCAA AGCTCAGAGC
    AACAGAGCAG ACGACCAGCG CGGGTTGCTG AGGAAGGAGG ACCTGGTGCT ACCCGAGTTT
    CTGCGCTTAC CTCCCGGCCC CATGGAGCTT GCCCTTTCCT CACCAAGCGC GGCCAAGAGC
    TTTGGCAAGA GGAGCTTGAC GGGCAACGGC AAGGAAAAGG CTCCCCAGCC CCAGGAGGCA
    TGGGGGCCCG GCCAAGACTT CCGCGAAAGC CCAGCAACAA GCCCCAGCTC GGCTGACAGC
    ACCCCGTTCA GCGCCCCCCT GACCCCCTCG CCCCCAACAC AGGAAGCTGA GGCCGGGGGT
    GTCCAGACCC TGGAGGGCGA ACACGTGGCC GACCTGACGC TCACGGGGGA GGGGGACATG
    AGCAGCCCCA ACAGCACTTT ACTGCCGCCA ACCCCCACCC CGCAGGACTC GGCCGGACCT
    CCGAGACCAG ACACACCGGG AGTTCATGGG CACCCTCTGG CATTTCCTGT CCTGGGAAGG
    ACCAGCTGGG GAGGAGGCGG GGCCAGAGGC AGCCAAGGCC TCCCAGTCAA CACCATCATC
    GACGTGGACC GTGTGGCTGG CTTGGCACCC GGGCAAGTCA GCAGCATCCC CAGGGTGCGG
    ACAGGTCCCC AGAGGTCACA GGCCAGTGGG CCCCTGACAT CAGAGCTCCC CAGCCTGAGC
    CCCGTCCCAG CTGGGCCTAC CAAGTTCAAG GCCAGCCAGC ACCATGCAAC CTTCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968009.1
    CDS121..4440
    Translation

    Target ORF information:

    RefSeq Version XM_014112534.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112534.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCAG CTGAGTGGAA AATCGAAATC AGGAAGATCC
    CTGAACGAAG AGCTGGGGGA TGAGGATAGT GAGAAGAAGC GGAAAGGAGC CTTCTTCTCT
    TGGTCAAGGA CCAGGAGCAC TGGGCGGTCC CAGAAGAAGA AGGAGCATGG TGACCACACA
    AACGAGCCCC TGCATGCCAA TGGAGGCCTG GGCCGCCGCG AGTCGCAGGG CTCCGTGTCC
    TCTGCGGGGA GTCTGGACCT GTCGGATGCC TGTAGGACCT TGATGCCAGA GCGGGACAAG
    GCCGCCAAGC ACTGCTGCAT CCAGCTCCCC GATGGGACGT CCTGCATGGT GGTCGTCAAG
    GCAGGCTTAT CCATCAAGGA GGTCCTGGCT GGGCTCTGCG AGCGACATGG CATCAACGGG
    GCTGCCGTAG ACCTCTTCCT GGTGGGCGGG GACAAGCCTC TGGTGCTGCA TCAAGACAGT
    AGCATCTTGG AGTCTCGGGA CCTGCGGCTA GAAAAGCGGA CTTTGTTTCG GCTGGATCTG
    GTTCCAATTA ACCGGTCAGT GGGACTGAAG GCCAAGCCGA CCAAGCCTGT CACAGAAGTA
    CTGCGGCCCG TGGTAGCCAA GTATGGCCTG CAGCTGAACG AGCTGGTGGC CAGGCTGAGT
    GGAGAGAAGG AGCCCCTGGA CCTTGGTGCC CCCATATCAA GTCTGGATGG GCAGAGGGTC
    ATCTTGGAGG AGAAGGAGCC TTCCAGGGGA AAAGGATCCA CAGATAAACA GAAAAGTGCA
    CCAGCCAAAC AGAATATAGC TGTAAATTCC AGTTCCAGAA ATCACTCGGC TACGGGAGAG
    GAGAGAACGC TAGGCAAGTC TAATTCTATT AAAATAAAAG GAGAAAATGG AAAAAATGCT
    AGGGATCCCC GGCTTTCAAA GAGAGAAGAA TCTATTGCAA AGATTGGGAA AAAAAAATAT
    CAGAAAATTA ATTTGGACGA AGCAGAGGAG TTTTTTGAGC TCATTTCCAA AGCTCAGAGC
    AACAGAGCAG ACGACCAGCG CGGGTTGCTG AGGAAGGAGG ACCTGGTGCT ACCCGAGTTT
    CTGCGCTTAC CTCCCGGCCC CATGGAGCTT GCCCTTTCCT CACCAAGCGC GGCCAAGAGC
    TTTGGCAAGA GGAGCTTGAC GGGCAACGGC AAGGAAAAGG CTCCCCAGCC CCAGGAGGCA
    TGGGGGCCCG GCCAAGACTT CCGCGAAAGC CCAGCAACAA GCCCCAGCTC GGCTGACAGC
    ACCCCGTTCA GCGCCCCCCT GACCCCCTCG CCCCCAACAC AGGAAGCTGA GGCCGGGGGT
    GTCCAGACCC TGGAGGGCGA ACACGTGGCC GACCTGACGC TCACGGGGGA GGGGGACATG
    AGCAGCCCCA ACAGCACTTT ACTGCCGCCA ACCCCCACCC CGCAGGACTC GGCCGGACCT
    CCGAGACCAG ACACACCGGG AGTTCATGGG CACCCTCTGG CATTTCCTGT CCTGGGAAGG
    ACCAGCTGGG GAGGAGGCGG GGCCAGAGGC AGCCAAGGCC TCCCAGTCAA CACCATCATC
    GACGTGGACC GTGTGGCTGG CTTGGCACCC GGGCAAGTCA GCAGCATCCC CAGGGTGCGG
    ACAGGTCCCC AGAGGTCACA GGCCAGTGGG CCCCTGACAT CAGAGCTCCC CAGCCTGAGC
    CCCGTCCCAG CTGGGCCTAC CAAGTTCAAG GCCAGCCAGC ACCATGCAAC CTTCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35796
    Clone ID Related Accession (Same CDS sequence) XM_014112534.2 , XM_014112534.1
    Accession Version XM_014112534.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 12 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCAG CTGAGTGGAA AATCGAAATC AGGAAGATCC
    CTGAACGAAG AGCTGGGGGA TGAGGATAGT GAGAAGAAGC GGAAAGGAGC CTTCTTCTCT
    TGGTCAAGGA CCAGGAGCAC TGGGCGGTCC CAGAAGAAGA AGGAGCATGG TGACCACACA
    AACGAGCCCC TGCATGCCAA TGGAGGCCTG GGCCGCCGCG AGTCGCAGGG CTCCGTGTCC
    TCTGCGGGGA GTCTGGACCT GTCGGATGCC TGTAGGACCT TGATGCCAGA GCGGGACAAG
    GCCGCCAAGC ACTGCTGCAT CCAGCTCCCC GATGGGACGT CCTGCATGGT GGTCGTCAAG
    GCAGGCTTAT CCATCAAGGA GGTCCTGGCT GGGCTCTGCG AGCGACATGG CATCAACGGG
    GCTGCCGTAG ACCTCTTCCT GGTGGGCGGG GACAAGCCTC TGGTGCTGCA TCAAGACAGT
    AGCATCTTGG AGTCTCGGGA CCTGCGGCTA GAAAAGCGGA CTTTGTTTCG GCTGGATCTG
    GTTCCAATTA ACCGGTCAGT GGGACTGAAG GCCAAGCCGA CCAAGCCTGT CACAGAAGTA
    CTGCGGCCCG TGGTAGCCAA GTATGGCCTG CAGCTGAACG AGCTGGTGGC CAGGCTGAGT
    GGAGAGAAGG AGCCCCTGGA CCTTGGTGCC CCCATATCAA GTCTGGATGG GCAGAGGGTC
    ATCTTGGAGG AGAAGGAGCC TTCCAGGGGA AAAGGATCCA CAGATAAACA GAAAAGTGCA
    CCAGCCAAAC AGAATATAGC TGTAAATTCC AGTTCCAGAA ATCACTCGGC TACGGGAGAG
    GAGAGAACGC TAGGCAAGTC TAATTCTATT AAAATAAAAG GAGAAAATGG AAAAAATGCT
    AGGGATCCCC GGCTTTCAAA GAGAGAAGAA TCTATTGCAA AGATTGGGAA AAAAAAATAT
    CAGAAAATTA ATTTGGACGA AGCAGAGGAG TTTTTTGAGC TCATTTCCAA AGCTCAGAGC
    AACAGAGCAG ACGACCAGCG CGGGTTGCTG AGGAAGGAGG ACCTGGTGCT ACCCGAGTTT
    CTGCGCTTAC CTCCCGGCCC CATGGAGCTT GCCCTTTCCT CACCAAGCGC GGCCAAGAGC
    TTTGGCAAGA GGAGCTTGAC GGGCAACGGC AAGGAAAAGG CTCCCCAGCC CCAGGAGGCA
    TGGGGGCCCG GCCAAGACTT CCGCGAAAGC CCAGCAACAA GCCCCAGCTC GGCTGACAGC
    ACCCCGTTCA GCGCCCCCCT GACCCCCTCG CCCCCAACAC AGGAAGCTGA GGCCGGGGGT
    GTCCAGACCC TGGAGGGCGA ACACGTGGCC GACCTGACGC TCACGGGGGA GGGGGACATG
    AGCAGCCCCA ACAGCACTTT ACTGCCGCCA ACCCCCACCC CGCAGGACTC GGCCGGACCT
    CCGAGACCAG ACACACCGGG AGTTCATGGG CACCCTCTGG CATTTCCTGT CCTGGGAAGG
    ACCAGCTGGG GAGGAGGCGG GGCCAGAGGC AGCCAAGGCC TCCCAGTCAA CACCATCATC
    GACGTGGACC GTGTGGCTGG CTTGGCACCC GGGCAAGTCA GCAGCATCCC CAGGGTGCGG
    ACAGGTCCCC AGAGGTCACA GGCCAGTGGG CCCCTGACAT CAGAGCTCCC CAGCCTGAGC
    CCCGTCCCAG CTGGGCCTAC CAAGTTCAAG GCCAGCCAGC ACCATGCAAC CTTCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968009.1
    CDS101..4420
    Translation

    Target ORF information:

    RefSeq Version XM_014112534.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCAG CTGAGTGGAA AATCGAAATC AGGAAGATCC
    CTGAACGAAG AGCTGGGGGA TGAGGATAGT GAGAAGAAGC GGAAAGGAGC CTTCTTCTCT
    TGGTCAAGGA CCAGGAGCAC TGGGCGGTCC CAGAAGAAGA AGGAGCATGG TGACCACACA
    AACGAGCCCC TGCATGCCAA TGGAGGCCTG GGCCGCCGCG AGTCGCAGGG CTCCGTGTCC
    TCTGCGGGGA GTCTGGACCT GTCGGATGCC TGTAGGACCT TGATGCCAGA GCGGGACAAG
    GCCGCCAAGC ACTGCTGCAT CCAGCTCCCC GATGGGACGT CCTGCATGGT GGTCGTCAAG
    GCAGGCTTAT CCATCAAGGA GGTCCTGGCT GGGCTCTGCG AGCGACATGG CATCAACGGG
    GCTGCCGTAG ACCTCTTCCT GGTGGGCGGG GACAAGCCTC TGGTGCTGCA TCAAGACAGT
    AGCATCTTGG AGTCTCGGGA CCTGCGGCTA GAAAAGCGGA CTTTGTTTCG GCTGGATCTG
    GTTCCAATTA ACCGGTCAGT GGGACTGAAG GCCAAGCCGA CCAAGCCTGT CACAGAAGTA
    CTGCGGCCCG TGGTAGCCAA GTATGGCCTG CAGCTGAACG AGCTGGTGGC CAGGCTGAGT
    GGAGAGAAGG AGCCCCTGGA CCTTGGTGCC CCCATATCAA GTCTGGATGG GCAGAGGGTC
    ATCTTGGAGG AGAAGGAGCC TTCCAGGGGA AAAGGATCCA CAGATAAACA GAAAAGTGCA
    CCAGCCAAAC AGAATATAGC TGTAAATTCC AGTTCCAGAA ATCACTCGGC TACGGGAGAG
    GAGAGAACGC TAGGCAAGTC TAATTCTATT AAAATAAAAG GAGAAAATGG AAAAAATGCT
    AGGGATCCCC GGCTTTCAAA GAGAGAAGAA TCTATTGCAA AGATTGGGAA AAAAAAATAT
    CAGAAAATTA ATTTGGACGA AGCAGAGGAG TTTTTTGAGC TCATTTCCAA AGCTCAGAGC
    AACAGAGCAG ACGACCAGCG CGGGTTGCTG AGGAAGGAGG ACCTGGTGCT ACCCGAGTTT
    CTGCGCTTAC CTCCCGGCCC CATGGAGCTT GCCCTTTCCT CACCAAGCGC GGCCAAGAGC
    TTTGGCAAGA GGAGCTTGAC GGGCAACGGC AAGGAAAAGG CTCCCCAGCC CCAGGAGGCA
    TGGGGGCCCG GCCAAGACTT CCGCGAAAGC CCAGCAACAA GCCCCAGCTC GGCTGACAGC
    ACCCCGTTCA GCGCCCCCCT GACCCCCTCG CCCCCAACAC AGGAAGCTGA GGCCGGGGGT
    GTCCAGACCC TGGAGGGCGA ACACGTGGCC GACCTGACGC TCACGGGGGA GGGGGACATG
    AGCAGCCCCA ACAGCACTTT ACTGCCGCCA ACCCCCACCC CGCAGGACTC GGCCGGACCT
    CCGAGACCAG ACACACCGGG AGTTCATGGG CACCCTCTGG CATTTCCTGT CCTGGGAAGG
    ACCAGCTGGG GAGGAGGCGG GGCCAGAGGC AGCCAAGGCC TCCCAGTCAA CACCATCATC
    GACGTGGACC GTGTGGCTGG CTTGGCACCC GGGCAAGTCA GCAGCATCCC CAGGGTGCGG
    ACAGGTCCCC AGAGGTCACA GGCCAGTGGG CCCCTGACAT CAGAGCTCCC CAGCCTGAGC
    CCCGTCCCAG CTGGGCCTAC CAAGTTCAAG GCCAGCCAGC ACCATGCAAC CTTCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35797
    Clone ID Related Accession (Same CDS sequence) XM_014112536.1 , XM_014112536.2
    Accession Version XM_014112536.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4257bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 12 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCAG CTGAGTGGAA AATCGAAATC AGGAAGATCC
    CTGAACGAAG AGCTGGGGGA TGAGGATAGT GAGAAGAAGC GGAAAGGAGC CTTCTTCTCT
    TGGTCAAGGA CCAGGAGCAC TGGGCGGTCC CAGAAGAAGA AGGAGCATGG TGACCACACA
    AACGAGCCCC TGCATGCCAA TGGAGGCCTG GGCCGCCGCG AGTCGCAGGG CTCCGTGTCC
    TCTGCGGGGA GTCTGGACCT GTCGGATGCC TGTAGGACCT TGATGCCAGA GCGGGACAAG
    GCCGCCAAGC ACTGCTGCAT CCAGCTCCCC GATGGGACGT CCTGCATGGT GGTCGTCAAG
    GCAGGCTTAT CCATCAAGGA GGTCCTGGCT GGGCTCTGCG AGCGACATGG CATCAACGGG
    GCTGCCGTAG ACCTCTTCCT GGTGGGCGGG GACAAGCCTC TGGTGCTGCA TCAAGACAGT
    AGCATCTTGG AGTCTCGGGA CCTGCGGCTA GAAAAGCGGA CTTTGTTTCG GCTGGATCTG
    GTTCCAATTA ACCGGTCAGT GGGACTGAAG GCCAAGCCGA CCAAGCCTGT CACAGAAGTA
    CTGCGGCCCG TGGTAGCCAA GTATGGCCTG CAGCTGAACG AGCTGGTGGC CAGGCTGAGT
    GGAGAGAAGG AGCCCCTGGA CCTTGGTGCC CCCATATCAA GTCTGGATGG GCAGAGGGTC
    ATCTTGGAGG AGAAGGAGCC TTCCAGGGGA AAAGGATCCA CAGATAAACA GAAAAGTGCA
    CCAGCCAAAC AGAATATAGC TGTAAATTCC AGTTCCAGAA ATCACTCGGC TACGGGAGAG
    GAGAGAACGC TAGGCAAGTC TAATTCTATT AAAATAAAAG GAGAAAATGG AAAAAATGCT
    AGGGATCCCC GGCTTTCAAA GAGAGAAGAA TCTATTGCAA AGATTGGGAA AAAAAAATAT
    CAGAAAATTA ATTTGGACGA AGCAGAGGAG TTTTTTGAGC TCATTTCCAA AGCTCAGAGC
    AACAGAGCAG ACGACCAGCG CGGGTTGCTG AGGAAGGAGG ACCTGGTGCT ACCCGAGTTT
    CTGCGCTTAC CTCCCGGCCC CATGGAGCTT GCCCTTTCCT CACCAAGCGC GGCCAAGAGC
    TTTGGCAAGA GGAGCTTGAC GGGCAACGGC AAGGAAAAGG CTCCCCAGCC CCAGGAGGCA
    TGGGGGCCCG GCCAAGACTT CCGCGAAAGC CCAGCAACAA GCCCCAGCTC GGCTGACAGC
    ACCCCGTTCA GCGCCCCCCT GACCCCCTCG CCCCCAACAC AGGAAGCTGA GGCCGGGGGT
    GTCCAGACCC TGGAGGGCGA ACACGTGGCC GACCTGACGC TCACGGGGGA GGGGGACATG
    AGCAGCCCCA ACAGCACTTT ACTGCCGCCA ACCCCCACCC CGCAGGACTC GGCCGGACCT
    CCGAGACCAG GAGGCGGGGC CAGAGGCAGC CAAGGCCTCC CAGTCAACAC CATCATCGAC
    GTGGACCGTG TGGCTGGCTT GGCACCCGGG CAAGTCAGCA GCATCCCCAG GGTGCGGACA
    GGTCCCCAGA GGTCACAGGC CAGTGGGCCC CTGACATCAG AGCTCCCCAG CCTGAGCCCC
    GTCCCAGCTG GGCCTACCAA GTTCAAGGCC AGCCAGCACC ATGCAACCTT CGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968011.1
    CDS101..4357
    Translation

    Target ORF information:

    RefSeq Version XM_014112536.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCAG CTGAGTGGAA AATCGAAATC AGGAAGATCC
    CTGAACGAAG AGCTGGGGGA TGAGGATAGT GAGAAGAAGC GGAAAGGAGC CTTCTTCTCT
    TGGTCAAGGA CCAGGAGCAC TGGGCGGTCC CAGAAGAAGA AGGAGCATGG TGACCACACA
    AACGAGCCCC TGCATGCCAA TGGAGGCCTG GGCCGCCGCG AGTCGCAGGG CTCCGTGTCC
    TCTGCGGGGA GTCTGGACCT GTCGGATGCC TGTAGGACCT TGATGCCAGA GCGGGACAAG
    GCCGCCAAGC ACTGCTGCAT CCAGCTCCCC GATGGGACGT CCTGCATGGT GGTCGTCAAG
    GCAGGCTTAT CCATCAAGGA GGTCCTGGCT GGGCTCTGCG AGCGACATGG CATCAACGGG
    GCTGCCGTAG ACCTCTTCCT GGTGGGCGGG GACAAGCCTC TGGTGCTGCA TCAAGACAGT
    AGCATCTTGG AGTCTCGGGA CCTGCGGCTA GAAAAGCGGA CTTTGTTTCG GCTGGATCTG
    GTTCCAATTA ACCGGTCAGT GGGACTGAAG GCCAAGCCGA CCAAGCCTGT CACAGAAGTA
    CTGCGGCCCG TGGTAGCCAA GTATGGCCTG CAGCTGAACG AGCTGGTGGC CAGGCTGAGT
    GGAGAGAAGG AGCCCCTGGA CCTTGGTGCC CCCATATCAA GTCTGGATGG GCAGAGGGTC
    ATCTTGGAGG AGAAGGAGCC TTCCAGGGGA AAAGGATCCA CAGATAAACA GAAAAGTGCA
    CCAGCCAAAC AGAATATAGC TGTAAATTCC AGTTCCAGAA ATCACTCGGC TACGGGAGAG
    GAGAGAACGC TAGGCAAGTC TAATTCTATT AAAATAAAAG GAGAAAATGG AAAAAATGCT
    AGGGATCCCC GGCTTTCAAA GAGAGAAGAA TCTATTGCAA AGATTGGGAA AAAAAAATAT
    CAGAAAATTA ATTTGGACGA AGCAGAGGAG TTTTTTGAGC TCATTTCCAA AGCTCAGAGC
    AACAGAGCAG ACGACCAGCG CGGGTTGCTG AGGAAGGAGG ACCTGGTGCT ACCCGAGTTT
    CTGCGCTTAC CTCCCGGCCC CATGGAGCTT GCCCTTTCCT CACCAAGCGC GGCCAAGAGC
    TTTGGCAAGA GGAGCTTGAC GGGCAACGGC AAGGAAAAGG CTCCCCAGCC CCAGGAGGCA
    TGGGGGCCCG GCCAAGACTT CCGCGAAAGC CCAGCAACAA GCCCCAGCTC GGCTGACAGC
    ACCCCGTTCA GCGCCCCCCT GACCCCCTCG CCCCCAACAC AGGAAGCTGA GGCCGGGGGT
    GTCCAGACCC TGGAGGGCGA ACACGTGGCC GACCTGACGC TCACGGGGGA GGGGGACATG
    AGCAGCCCCA ACAGCACTTT ACTGCCGCCA ACCCCCACCC CGCAGGACTC GGCCGGACCT
    CCGAGACCAG GAGGCGGGGC CAGAGGCAGC CAAGGCCTCC CAGTCAACAC CATCATCGAC
    GTGGACCGTG TGGCTGGCTT GGCACCCGGG CAAGTCAGCA GCATCCCCAG GGTGCGGACA
    GGTCCCCAGA GGTCACAGGC CAGTGGGCCC CTGACATCAG AGCTCCCCAG CCTGAGCCCC
    GTCCCAGCTG GGCCTACCAA GTTCAAGGCC AGCCAGCACC ATGCAACCTT CGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35797
    Clone ID Related Accession (Same CDS sequence) XM_014112536.1 , XM_014112536.2
    Accession Version XM_014112536.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4257bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 12 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCAG CTGAGTGGAA AATCGAAATC AGGAAGATCC
    CTGAACGAAG AGCTGGGGGA TGAGGATAGT GAGAAGAAGC GGAAAGGAGC CTTCTTCTCT
    TGGTCAAGGA CCAGGAGCAC TGGGCGGTCC CAGAAGAAGA AGGAGCATGG TGACCACACA
    AACGAGCCCC TGCATGCCAA TGGAGGCCTG GGCCGCCGCG AGTCGCAGGG CTCCGTGTCC
    TCTGCGGGGA GTCTGGACCT GTCGGATGCC TGTAGGACCT TGATGCCAGA GCGGGACAAG
    GCCGCCAAGC ACTGCTGCAT CCAGCTCCCC GATGGGACGT CCTGCATGGT GGTCGTCAAG
    GCAGGCTTAT CCATCAAGGA GGTCCTGGCT GGGCTCTGCG AGCGACATGG CATCAACGGG
    GCTGCCGTAG ACCTCTTCCT GGTGGGCGGG GACAAGCCTC TGGTGCTGCA TCAAGACAGT
    AGCATCTTGG AGTCTCGGGA CCTGCGGCTA GAAAAGCGGA CTTTGTTTCG GCTGGATCTG
    GTTCCAATTA ACCGGTCAGT GGGACTGAAG GCCAAGCCGA CCAAGCCTGT CACAGAAGTA
    CTGCGGCCCG TGGTAGCCAA GTATGGCCTG CAGCTGAACG AGCTGGTGGC CAGGCTGAGT
    GGAGAGAAGG AGCCCCTGGA CCTTGGTGCC CCCATATCAA GTCTGGATGG GCAGAGGGTC
    ATCTTGGAGG AGAAGGAGCC TTCCAGGGGA AAAGGATCCA CAGATAAACA GAAAAGTGCA
    CCAGCCAAAC AGAATATAGC TGTAAATTCC AGTTCCAGAA ATCACTCGGC TACGGGAGAG
    GAGAGAACGC TAGGCAAGTC TAATTCTATT AAAATAAAAG GAGAAAATGG AAAAAATGCT
    AGGGATCCCC GGCTTTCAAA GAGAGAAGAA TCTATTGCAA AGATTGGGAA AAAAAAATAT
    CAGAAAATTA ATTTGGACGA AGCAGAGGAG TTTTTTGAGC TCATTTCCAA AGCTCAGAGC
    AACAGAGCAG ACGACCAGCG CGGGTTGCTG AGGAAGGAGG ACCTGGTGCT ACCCGAGTTT
    CTGCGCTTAC CTCCCGGCCC CATGGAGCTT GCCCTTTCCT CACCAAGCGC GGCCAAGAGC
    TTTGGCAAGA GGAGCTTGAC GGGCAACGGC AAGGAAAAGG CTCCCCAGCC CCAGGAGGCA
    TGGGGGCCCG GCCAAGACTT CCGCGAAAGC CCAGCAACAA GCCCCAGCTC GGCTGACAGC
    ACCCCGTTCA GCGCCCCCCT GACCCCCTCG CCCCCAACAC AGGAAGCTGA GGCCGGGGGT
    GTCCAGACCC TGGAGGGCGA ACACGTGGCC GACCTGACGC TCACGGGGGA GGGGGACATG
    AGCAGCCCCA ACAGCACTTT ACTGCCGCCA ACCCCCACCC CGCAGGACTC GGCCGGACCT
    CCGAGACCAG GAGGCGGGGC CAGAGGCAGC CAAGGCCTCC CAGTCAACAC CATCATCGAC
    GTGGACCGTG TGGCTGGCTT GGCACCCGGG CAAGTCAGCA GCATCCCCAG GGTGCGGACA
    GGTCCCCAGA GGTCACAGGC CAGTGGGCCC CTGACATCAG AGCTCCCCAG CCTGAGCCCC
    GTCCCAGCTG GGCCTACCAA GTTCAAGGCC AGCCAGCACC ATGCAACCTT CGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968011.1
    CDS121..4377
    Translation

    Target ORF information:

    RefSeq Version XM_014112536.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112536.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCAG CTGAGTGGAA AATCGAAATC AGGAAGATCC
    CTGAACGAAG AGCTGGGGGA TGAGGATAGT GAGAAGAAGC GGAAAGGAGC CTTCTTCTCT
    TGGTCAAGGA CCAGGAGCAC TGGGCGGTCC CAGAAGAAGA AGGAGCATGG TGACCACACA
    AACGAGCCCC TGCATGCCAA TGGAGGCCTG GGCCGCCGCG AGTCGCAGGG CTCCGTGTCC
    TCTGCGGGGA GTCTGGACCT GTCGGATGCC TGTAGGACCT TGATGCCAGA GCGGGACAAG
    GCCGCCAAGC ACTGCTGCAT CCAGCTCCCC GATGGGACGT CCTGCATGGT GGTCGTCAAG
    GCAGGCTTAT CCATCAAGGA GGTCCTGGCT GGGCTCTGCG AGCGACATGG CATCAACGGG
    GCTGCCGTAG ACCTCTTCCT GGTGGGCGGG GACAAGCCTC TGGTGCTGCA TCAAGACAGT
    AGCATCTTGG AGTCTCGGGA CCTGCGGCTA GAAAAGCGGA CTTTGTTTCG GCTGGATCTG
    GTTCCAATTA ACCGGTCAGT GGGACTGAAG GCCAAGCCGA CCAAGCCTGT CACAGAAGTA
    CTGCGGCCCG TGGTAGCCAA GTATGGCCTG CAGCTGAACG AGCTGGTGGC CAGGCTGAGT
    GGAGAGAAGG AGCCCCTGGA CCTTGGTGCC CCCATATCAA GTCTGGATGG GCAGAGGGTC
    ATCTTGGAGG AGAAGGAGCC TTCCAGGGGA AAAGGATCCA CAGATAAACA GAAAAGTGCA
    CCAGCCAAAC AGAATATAGC TGTAAATTCC AGTTCCAGAA ATCACTCGGC TACGGGAGAG
    GAGAGAACGC TAGGCAAGTC TAATTCTATT AAAATAAAAG GAGAAAATGG AAAAAATGCT
    AGGGATCCCC GGCTTTCAAA GAGAGAAGAA TCTATTGCAA AGATTGGGAA AAAAAAATAT
    CAGAAAATTA ATTTGGACGA AGCAGAGGAG TTTTTTGAGC TCATTTCCAA AGCTCAGAGC
    AACAGAGCAG ACGACCAGCG CGGGTTGCTG AGGAAGGAGG ACCTGGTGCT ACCCGAGTTT
    CTGCGCTTAC CTCCCGGCCC CATGGAGCTT GCCCTTTCCT CACCAAGCGC GGCCAAGAGC
    TTTGGCAAGA GGAGCTTGAC GGGCAACGGC AAGGAAAAGG CTCCCCAGCC CCAGGAGGCA
    TGGGGGCCCG GCCAAGACTT CCGCGAAAGC CCAGCAACAA GCCCCAGCTC GGCTGACAGC
    ACCCCGTTCA GCGCCCCCCT GACCCCCTCG CCCCCAACAC AGGAAGCTGA GGCCGGGGGT
    GTCCAGACCC TGGAGGGCGA ACACGTGGCC GACCTGACGC TCACGGGGGA GGGGGACATG
    AGCAGCCCCA ACAGCACTTT ACTGCCGCCA ACCCCCACCC CGCAGGACTC GGCCGGACCT
    CCGAGACCAG GAGGCGGGGC CAGAGGCAGC CAAGGCCTCC CAGTCAACAC CATCATCGAC
    GTGGACCGTG TGGCTGGCTT GGCACCCGGG CAAGTCAGCA GCATCCCCAG GGTGCGGACA
    GGTCCCCAGA GGTCACAGGC CAGTGGGCCC CTGACATCAG AGCTCCCCAG CCTGAGCCCC
    GTCCCAGCTG GGCCTACCAA GTTCAAGGCC AGCCAGCACC ATGCAACCTT CGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35798
    Clone ID Related Accession (Same CDS sequence) XM_014112538.1 , XM_014112538.2
    Accession Version XM_014112538.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 12 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGCA GCTGAGTGGA AAATCGAAAT CAGGAAGATC CCTGAACGAA
    GAGCTGGGGG ATGAGGATAG TGAGAAGAAG CGGAAAGGAG CCTTCTTCTC TTGGTCAAGG
    ACCAGGAGCA CTGGGCGGTC CCAGAAGAAG AAGGAGCATG GTGACCACAC AAACGAGCCC
    CTGCATGCCA ATGGAGGCCT GGGCCGCCGC GAGTCGCAGG GCTCCGTGTC CTCTGCGGGG
    AGTCTGGACC TGTCGGATGC CTGTAGGACC TTGATGCCAG AGCGGGACAA GGCCGCCAAG
    CACTGCTGCA TCCAGCTCCC CGATGGGACG TCCTGCATGG TGGTCGTCAA GGCAGGCTTA
    TCCATCAAGG AGGTCCTGGC TGGGCTCTGC GAGCGACATG GCATCAACGG GGCTGCCGTA
    GACCTCTTCC TGGTGGGCGG GGACAAGCCT CTGGTGCTGC ATCAAGACAG TAGCATCTTG
    GAGTCTCGGG ACCTGCGGCT AGAAAAGCGG ACTTTGTTTC GGCTGGATCT GGTTCCAATT
    AACCGGTCAG TGGGACTGAA GGCCAAGCCG ACCAAGCCTG TCACAGAAGT ACTGCGGCCC
    GTGGTAGCCA AGTATGGCCT GCAGCTGAAC GAGCTGGTGG CCAGGCTGAG TGGAGAGAAG
    GAGCCCCTGG ACCTTGGTGC CCCCATATCA AGTCTGGATG GGCAGAGGGT CATCTTGGAG
    GAGAAGGAGC CTTCCAGGGG AAAAGGATCC ACAGATAAAC AGAAAAGTGC ACCAGCCAAA
    CAGAATATAG CTGTAAATTC CAGTTCCAGA AATCACTCGG CTACGGGAGA GGAGAGAACG
    CTAGGCAAGT CTAATTCTAT TAAAATAAAA GGAGAAAATG GAAAAAATGC TAGGGATCCC
    CGGCTTTCAA AGAGAGAAGA ATCTATTGCA AAGATTGGGA AAAAAAAATA TCAGAAAATT
    AATTTGGACG AAGCAGAGGA GTTTTTTGAG CTCATTTCCA AAGCTCAGAG CAACAGAGCA
    GACGACCAGC GCGGGTTGCT GAGGAAGGAG GACCTGGTGC TACCCGAGTT TCTGCGCTTA
    CCTCCCGGCC CCATGGAGCT TGCCCTTTCC TCACCAAGCG CGGCCAAGAG CTTTGGCAAG
    AGGAGCTTGA CGGGCAACGG CAAGGAAAAG GCTCCCCAGC CCCAGGAGGC ATGGGGGCCC
    GGCCAAGACT TCCGCGAAAG CCCAGCAACA AGCCCCAGCT CGGCTGACAG CACCCCGTTC
    AGCGCCCCCC TGACCCCCTC GCCCCCAACA CAGGAAGCTG AGGCCGGGGG TGTCCAGACC
    CTGGAGGGCG AACACGTGGC CGACCTGACG CTCACGGGGG AGGGGGACAT GAGCAGCCCC
    AACAGCACTT TACTGCCGCC AACCCCCACC CCGCAGGACT CGGCCGGACC TCCGAGACCA
    GACACACCGG GAGTTCATGG GCACCCTCTG GCATTTCCTG TCCTGGGAAG GACCAGCTGG
    GGAGGAGGCG GGGCCAGAGG CAGCCAAGGC CTCCCAGTCA ACACCATCAT CGACGTGGAC
    CGTGTGGCTG GCTTGGCACC CGGGCAAGTC AGCAGCATCC CCAGGGTGCG GACAGGTCCC
    CAGAGGTCAC AGGCCAGTGG GCCCCTGACA TCAGAGCTCC CCAGCCTGAG CCCCGTCCCA
    GCTGGGCCTA CCAAGTTCAA GGCCAGCCAG CACCATGCAA CCTTCGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968013.1
    CDS101..4351
    Translation

    Target ORF information:

    RefSeq Version XM_014112538.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112538.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGCA GCTGAGTGGA AAATCGAAAT CAGGAAGATC CCTGAACGAA
    GAGCTGGGGG ATGAGGATAG TGAGAAGAAG CGGAAAGGAG CCTTCTTCTC TTGGTCAAGG
    ACCAGGAGCA CTGGGCGGTC CCAGAAGAAG AAGGAGCATG GTGACCACAC AAACGAGCCC
    CTGCATGCCA ATGGAGGCCT GGGCCGCCGC GAGTCGCAGG GCTCCGTGTC CTCTGCGGGG
    AGTCTGGACC TGTCGGATGC CTGTAGGACC TTGATGCCAG AGCGGGACAA GGCCGCCAAG
    CACTGCTGCA TCCAGCTCCC CGATGGGACG TCCTGCATGG TGGTCGTCAA GGCAGGCTTA
    TCCATCAAGG AGGTCCTGGC TGGGCTCTGC GAGCGACATG GCATCAACGG GGCTGCCGTA
    GACCTCTTCC TGGTGGGCGG GGACAAGCCT CTGGTGCTGC ATCAAGACAG TAGCATCTTG
    GAGTCTCGGG ACCTGCGGCT AGAAAAGCGG ACTTTGTTTC GGCTGGATCT GGTTCCAATT
    AACCGGTCAG TGGGACTGAA GGCCAAGCCG ACCAAGCCTG TCACAGAAGT ACTGCGGCCC
    GTGGTAGCCA AGTATGGCCT GCAGCTGAAC GAGCTGGTGG CCAGGCTGAG TGGAGAGAAG
    GAGCCCCTGG ACCTTGGTGC CCCCATATCA AGTCTGGATG GGCAGAGGGT CATCTTGGAG
    GAGAAGGAGC CTTCCAGGGG AAAAGGATCC ACAGATAAAC AGAAAAGTGC ACCAGCCAAA
    CAGAATATAG CTGTAAATTC CAGTTCCAGA AATCACTCGG CTACGGGAGA GGAGAGAACG
    CTAGGCAAGT CTAATTCTAT TAAAATAAAA GGAGAAAATG GAAAAAATGC TAGGGATCCC
    CGGCTTTCAA AGAGAGAAGA ATCTATTGCA AAGATTGGGA AAAAAAAATA TCAGAAAATT
    AATTTGGACG AAGCAGAGGA GTTTTTTGAG CTCATTTCCA AAGCTCAGAG CAACAGAGCA
    GACGACCAGC GCGGGTTGCT GAGGAAGGAG GACCTGGTGC TACCCGAGTT TCTGCGCTTA
    CCTCCCGGCC CCATGGAGCT TGCCCTTTCC TCACCAAGCG CGGCCAAGAG CTTTGGCAAG
    AGGAGCTTGA CGGGCAACGG CAAGGAAAAG GCTCCCCAGC CCCAGGAGGC ATGGGGGCCC
    GGCCAAGACT TCCGCGAAAG CCCAGCAACA AGCCCCAGCT CGGCTGACAG CACCCCGTTC
    AGCGCCCCCC TGACCCCCTC GCCCCCAACA CAGGAAGCTG AGGCCGGGGG TGTCCAGACC
    CTGGAGGGCG AACACGTGGC CGACCTGACG CTCACGGGGG AGGGGGACAT GAGCAGCCCC
    AACAGCACTT TACTGCCGCC AACCCCCACC CCGCAGGACT CGGCCGGACC TCCGAGACCA
    GACACACCGG GAGTTCATGG GCACCCTCTG GCATTTCCTG TCCTGGGAAG GACCAGCTGG
    GGAGGAGGCG GGGCCAGAGG CAGCCAAGGC CTCCCAGTCA ACACCATCAT CGACGTGGAC
    CGTGTGGCTG GCTTGGCACC CGGGCAAGTC AGCAGCATCC CCAGGGTGCG GACAGGTCCC
    CAGAGGTCAC AGGCCAGTGG GCCCCTGACA TCAGAGCTCC CCAGCCTGAG CCCCGTCCCA
    GCTGGGCCTA CCAAGTTCAA GGCCAGCCAG CACCATGCAA CCTTCGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35798
    Clone ID Related Accession (Same CDS sequence) XM_014112538.1 , XM_014112538.2
    Accession Version XM_014112538.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 12 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112538.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGCA GCTGAGTGGA AAATCGAAAT CAGGAAGATC CCTGAACGAA
    GAGCTGGGGG ATGAGGATAG TGAGAAGAAG CGGAAAGGAG CCTTCTTCTC TTGGTCAAGG
    ACCAGGAGCA CTGGGCGGTC CCAGAAGAAG AAGGAGCATG GTGACCACAC AAACGAGCCC
    CTGCATGCCA ATGGAGGCCT GGGCCGCCGC GAGTCGCAGG GCTCCGTGTC CTCTGCGGGG
    AGTCTGGACC TGTCGGATGC CTGTAGGACC TTGATGCCAG AGCGGGACAA GGCCGCCAAG
    CACTGCTGCA TCCAGCTCCC CGATGGGACG TCCTGCATGG TGGTCGTCAA GGCAGGCTTA
    TCCATCAAGG AGGTCCTGGC TGGGCTCTGC GAGCGACATG GCATCAACGG GGCTGCCGTA
    GACCTCTTCC TGGTGGGCGG GGACAAGCCT CTGGTGCTGC ATCAAGACAG TAGCATCTTG
    GAGTCTCGGG ACCTGCGGCT AGAAAAGCGG ACTTTGTTTC GGCTGGATCT GGTTCCAATT
    AACCGGTCAG TGGGACTGAA GGCCAAGCCG ACCAAGCCTG TCACAGAAGT ACTGCGGCCC
    GTGGTAGCCA AGTATGGCCT GCAGCTGAAC GAGCTGGTGG CCAGGCTGAG TGGAGAGAAG
    GAGCCCCTGG ACCTTGGTGC CCCCATATCA AGTCTGGATG GGCAGAGGGT CATCTTGGAG
    GAGAAGGAGC CTTCCAGGGG AAAAGGATCC ACAGATAAAC AGAAAAGTGC ACCAGCCAAA
    CAGAATATAG CTGTAAATTC CAGTTCCAGA AATCACTCGG CTACGGGAGA GGAGAGAACG
    CTAGGCAAGT CTAATTCTAT TAAAATAAAA GGAGAAAATG GAAAAAATGC TAGGGATCCC
    CGGCTTTCAA AGAGAGAAGA ATCTATTGCA AAGATTGGGA AAAAAAAATA TCAGAAAATT
    AATTTGGACG AAGCAGAGGA GTTTTTTGAG CTCATTTCCA AAGCTCAGAG CAACAGAGCA
    GACGACCAGC GCGGGTTGCT GAGGAAGGAG GACCTGGTGC TACCCGAGTT TCTGCGCTTA
    CCTCCCGGCC CCATGGAGCT TGCCCTTTCC TCACCAAGCG CGGCCAAGAG CTTTGGCAAG
    AGGAGCTTGA CGGGCAACGG CAAGGAAAAG GCTCCCCAGC CCCAGGAGGC ATGGGGGCCC
    GGCCAAGACT TCCGCGAAAG CCCAGCAACA AGCCCCAGCT CGGCTGACAG CACCCCGTTC
    AGCGCCCCCC TGACCCCCTC GCCCCCAACA CAGGAAGCTG AGGCCGGGGG TGTCCAGACC
    CTGGAGGGCG AACACGTGGC CGACCTGACG CTCACGGGGG AGGGGGACAT GAGCAGCCCC
    AACAGCACTT TACTGCCGCC AACCCCCACC CCGCAGGACT CGGCCGGACC TCCGAGACCA
    GACACACCGG GAGTTCATGG GCACCCTCTG GCATTTCCTG TCCTGGGAAG GACCAGCTGG
    GGAGGAGGCG GGGCCAGAGG CAGCCAAGGC CTCCCAGTCA ACACCATCAT CGACGTGGAC
    CGTGTGGCTG GCTTGGCACC CGGGCAAGTC AGCAGCATCC CCAGGGTGCG GACAGGTCCC
    CAGAGGTCAC AGGCCAGTGG GCCCCTGACA TCAGAGCTCC CCAGCCTGAG CCCCGTCCCA
    GCTGGGCCTA CCAAGTTCAA GGCCAGCCAG CACCATGCAA CCTTCGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968013.1
    CDS122..4372
    Translation

    Target ORF information:

    RefSeq Version XM_014112538.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112538.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGCA GCTGAGTGGA AAATCGAAAT CAGGAAGATC CCTGAACGAA
    GAGCTGGGGG ATGAGGATAG TGAGAAGAAG CGGAAAGGAG CCTTCTTCTC TTGGTCAAGG
    ACCAGGAGCA CTGGGCGGTC CCAGAAGAAG AAGGAGCATG GTGACCACAC AAACGAGCCC
    CTGCATGCCA ATGGAGGCCT GGGCCGCCGC GAGTCGCAGG GCTCCGTGTC CTCTGCGGGG
    AGTCTGGACC TGTCGGATGC CTGTAGGACC TTGATGCCAG AGCGGGACAA GGCCGCCAAG
    CACTGCTGCA TCCAGCTCCC CGATGGGACG TCCTGCATGG TGGTCGTCAA GGCAGGCTTA
    TCCATCAAGG AGGTCCTGGC TGGGCTCTGC GAGCGACATG GCATCAACGG GGCTGCCGTA
    GACCTCTTCC TGGTGGGCGG GGACAAGCCT CTGGTGCTGC ATCAAGACAG TAGCATCTTG
    GAGTCTCGGG ACCTGCGGCT AGAAAAGCGG ACTTTGTTTC GGCTGGATCT GGTTCCAATT
    AACCGGTCAG TGGGACTGAA GGCCAAGCCG ACCAAGCCTG TCACAGAAGT ACTGCGGCCC
    GTGGTAGCCA AGTATGGCCT GCAGCTGAAC GAGCTGGTGG CCAGGCTGAG TGGAGAGAAG
    GAGCCCCTGG ACCTTGGTGC CCCCATATCA AGTCTGGATG GGCAGAGGGT CATCTTGGAG
    GAGAAGGAGC CTTCCAGGGG AAAAGGATCC ACAGATAAAC AGAAAAGTGC ACCAGCCAAA
    CAGAATATAG CTGTAAATTC CAGTTCCAGA AATCACTCGG CTACGGGAGA GGAGAGAACG
    CTAGGCAAGT CTAATTCTAT TAAAATAAAA GGAGAAAATG GAAAAAATGC TAGGGATCCC
    CGGCTTTCAA AGAGAGAAGA ATCTATTGCA AAGATTGGGA AAAAAAAATA TCAGAAAATT
    AATTTGGACG AAGCAGAGGA GTTTTTTGAG CTCATTTCCA AAGCTCAGAG CAACAGAGCA
    GACGACCAGC GCGGGTTGCT GAGGAAGGAG GACCTGGTGC TACCCGAGTT TCTGCGCTTA
    CCTCCCGGCC CCATGGAGCT TGCCCTTTCC TCACCAAGCG CGGCCAAGAG CTTTGGCAAG
    AGGAGCTTGA CGGGCAACGG CAAGGAAAAG GCTCCCCAGC CCCAGGAGGC ATGGGGGCCC
    GGCCAAGACT TCCGCGAAAG CCCAGCAACA AGCCCCAGCT CGGCTGACAG CACCCCGTTC
    AGCGCCCCCC TGACCCCCTC GCCCCCAACA CAGGAAGCTG AGGCCGGGGG TGTCCAGACC
    CTGGAGGGCG AACACGTGGC CGACCTGACG CTCACGGGGG AGGGGGACAT GAGCAGCCCC
    AACAGCACTT TACTGCCGCC AACCCCCACC CCGCAGGACT CGGCCGGACC TCCGAGACCA
    GACACACCGG GAGTTCATGG GCACCCTCTG GCATTTCCTG TCCTGGGAAG GACCAGCTGG
    GGAGGAGGCG GGGCCAGAGG CAGCCAAGGC CTCCCAGTCA ACACCATCAT CGACGTGGAC
    CGTGTGGCTG GCTTGGCACC CGGGCAAGTC AGCAGCATCC CCAGGGTGCG GACAGGTCCC
    CAGAGGTCAC AGGCCAGTGG GCCCCTGACA TCAGAGCTCC CCAGCCTGAG CCCCGTCCCA
    GCTGGGCCTA CCAAGTTCAA GGCCAGCCAG CACCATGCAA CCTTCGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35799
    Clone ID Related Accession (Same CDS sequence) XM_014112539.1 , XM_014112539.2
    Accession Version XM_014112539.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4248bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 12 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGCT GAGTGGAAAA TCGAAATCAG GAAGATCCCT GAACGAAGAG
    CTGGGGGATG AGGATAGTGA GAAGAAGCGG AAAGGAGCCT TCTTCTCTTG GTCAAGGACC
    AGGAGCACTG GGCGGTCCCA GAAGAAGAAG GAGCATGGTG ACCACACAAA CGAGCCCCTG
    CATGCCAATG GAGGCCTGGG CCGCCGCGAG TCGCAGGGCT CCGTGTCCTC TGCGGGGAGT
    CTGGACCTGT CGGATGCCTG TAGGACCTTG ATGCCAGAGC GGGACAAGGC CGCCAAGCAC
    TGCTGCATCC AGCTCCCCGA TGGGACGTCC TGCATGGTGG TCGTCAAGGC AGGCTTATCC
    ATCAAGGAGG TCCTGGCTGG GCTCTGCGAG CGACATGGCA TCAACGGGGC TGCCGTAGAC
    CTCTTCCTGG TGGGCGGGGA CAAGCCTCTG GTGCTGCATC AAGACAGTAG CATCTTGGAG
    TCTCGGGACC TGCGGCTAGA AAAGCGGACT TTGTTTCGGC TGGATCTGGT TCCAATTAAC
    CGGTCAGTGG GACTGAAGGC CAAGCCGACC AAGCCTGTCA CAGAAGTACT GCGGCCCGTG
    GTAGCCAAGT ATGGCCTGCA GCTGAACGAG CTGGTGGCCA GGCTGAGTGG AGAGAAGGAG
    CCCCTGGACC TTGGTGCCCC CATATCAAGT CTGGATGGGC AGAGGGTCAT CTTGGAGGAG
    AAGGAGCCTT CCAGGGGAAA AGGATCCACA GATAAACAGA AAAGTGCACC AGCCAAACAG
    AATATAGCTG TAAATTCCAG TTCCAGAAAT CACTCGGCTA CGGGAGAGGA GAGAACGCTA
    GGCAAGTCTA ATTCTATTAA AATAAAAGGA GAAAATGGAA AAAATGCTAG GGATCCCCGG
    CTTTCAAAGA GAGAAGAATC TATTGCAAAG ATTGGGAAAA AAAAATATCA GAAAATTAAT
    TTGGACGAAG CAGAGGAGTT TTTTGAGCTC ATTTCCAAAG CTCAGAGCAA CAGAGCAGAC
    GACCAGCGCG GGTTGCTGAG GAAGGAGGAC CTGGTGCTAC CCGAGTTTCT GCGCTTACCT
    CCCGGCCCCA TGGAGCTTGC CCTTTCCTCA CCAAGCGCGG CCAAGAGCTT TGGCAAGAGG
    AGCTTGACGG GCAACGGCAA GGAAAAGGCT CCCCAGCCCC AGGAGGCATG GGGGCCCGGC
    CAAGACTTCC GCGAAAGCCC AGCAACAAGC CCCAGCTCGG CTGACAGCAC CCCGTTCAGC
    GCCCCCCTGA CCCCCTCGCC CCCAACACAG GAAGCTGAGG CCGGGGGTGT CCAGACCCTG
    GAGGGCGAAC ACGTGGCCGA CCTGACGCTC ACGGGGGAGG GGGACATGAG CAGCCCCAAC
    AGCACTTTAC TGCCGCCAAC CCCCACCCCG CAGGACTCGG CCGGACCTCC GAGACCAGAC
    ACACCGGGAG TTCATGGGCA CCCTCTGGCA TTTCCTGTCC TGGGAAGGAC CAGCTGGGGA
    GGAGGCGGGG CCAGAGGCAG CCAAGGCCTC CCAGTCAACA CCATCATCGA CGTGGACCGT
    GTGGCTGGCT TGGCACCCGG GCAAGTCAGC AGCATCCCCA GGGTGCGGAC AGGTCCCCAG
    AGGTCACAGG CCAGTGGGCC CCTGACATCA GAGCTCCCCA GCCTGAGCCC CGTCCCAGCT
    GGGCCTACCA AGTTCAAGGC CAGCCAGCAC CATGCAACCT TCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968014.1
    CDS101..4348
    Translation

    Target ORF information:

    RefSeq Version XM_014112539.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGCT GAGTGGAAAA TCGAAATCAG GAAGATCCCT GAACGAAGAG
    CTGGGGGATG AGGATAGTGA GAAGAAGCGG AAAGGAGCCT TCTTCTCTTG GTCAAGGACC
    AGGAGCACTG GGCGGTCCCA GAAGAAGAAG GAGCATGGTG ACCACACAAA CGAGCCCCTG
    CATGCCAATG GAGGCCTGGG CCGCCGCGAG TCGCAGGGCT CCGTGTCCTC TGCGGGGAGT
    CTGGACCTGT CGGATGCCTG TAGGACCTTG ATGCCAGAGC GGGACAAGGC CGCCAAGCAC
    TGCTGCATCC AGCTCCCCGA TGGGACGTCC TGCATGGTGG TCGTCAAGGC AGGCTTATCC
    ATCAAGGAGG TCCTGGCTGG GCTCTGCGAG CGACATGGCA TCAACGGGGC TGCCGTAGAC
    CTCTTCCTGG TGGGCGGGGA CAAGCCTCTG GTGCTGCATC AAGACAGTAG CATCTTGGAG
    TCTCGGGACC TGCGGCTAGA AAAGCGGACT TTGTTTCGGC TGGATCTGGT TCCAATTAAC
    CGGTCAGTGG GACTGAAGGC CAAGCCGACC AAGCCTGTCA CAGAAGTACT GCGGCCCGTG
    GTAGCCAAGT ATGGCCTGCA GCTGAACGAG CTGGTGGCCA GGCTGAGTGG AGAGAAGGAG
    CCCCTGGACC TTGGTGCCCC CATATCAAGT CTGGATGGGC AGAGGGTCAT CTTGGAGGAG
    AAGGAGCCTT CCAGGGGAAA AGGATCCACA GATAAACAGA AAAGTGCACC AGCCAAACAG
    AATATAGCTG TAAATTCCAG TTCCAGAAAT CACTCGGCTA CGGGAGAGGA GAGAACGCTA
    GGCAAGTCTA ATTCTATTAA AATAAAAGGA GAAAATGGAA AAAATGCTAG GGATCCCCGG
    CTTTCAAAGA GAGAAGAATC TATTGCAAAG ATTGGGAAAA AAAAATATCA GAAAATTAAT
    TTGGACGAAG CAGAGGAGTT TTTTGAGCTC ATTTCCAAAG CTCAGAGCAA CAGAGCAGAC
    GACCAGCGCG GGTTGCTGAG GAAGGAGGAC CTGGTGCTAC CCGAGTTTCT GCGCTTACCT
    CCCGGCCCCA TGGAGCTTGC CCTTTCCTCA CCAAGCGCGG CCAAGAGCTT TGGCAAGAGG
    AGCTTGACGG GCAACGGCAA GGAAAAGGCT CCCCAGCCCC AGGAGGCATG GGGGCCCGGC
    CAAGACTTCC GCGAAAGCCC AGCAACAAGC CCCAGCTCGG CTGACAGCAC CCCGTTCAGC
    GCCCCCCTGA CCCCCTCGCC CCCAACACAG GAAGCTGAGG CCGGGGGTGT CCAGACCCTG
    GAGGGCGAAC ACGTGGCCGA CCTGACGCTC ACGGGGGAGG GGGACATGAG CAGCCCCAAC
    AGCACTTTAC TGCCGCCAAC CCCCACCCCG CAGGACTCGG CCGGACCTCC GAGACCAGAC
    ACACCGGGAG TTCATGGGCA CCCTCTGGCA TTTCCTGTCC TGGGAAGGAC CAGCTGGGGA
    GGAGGCGGGG CCAGAGGCAG CCAAGGCCTC CCAGTCAACA CCATCATCGA CGTGGACCGT
    GTGGCTGGCT TGGCACCCGG GCAAGTCAGC AGCATCCCCA GGGTGCGGAC AGGTCCCCAG
    AGGTCACAGG CCAGTGGGCC CCTGACATCA GAGCTCCCCA GCCTGAGCCC CGTCCCAGCT
    GGGCCTACCA AGTTCAAGGC CAGCCAGCAC CATGCAACCT TCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35799
    Clone ID Related Accession (Same CDS sequence) XM_014112539.1 , XM_014112539.2
    Accession Version XM_014112539.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4248bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 12 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGCT GAGTGGAAAA TCGAAATCAG GAAGATCCCT GAACGAAGAG
    CTGGGGGATG AGGATAGTGA GAAGAAGCGG AAAGGAGCCT TCTTCTCTTG GTCAAGGACC
    AGGAGCACTG GGCGGTCCCA GAAGAAGAAG GAGCATGGTG ACCACACAAA CGAGCCCCTG
    CATGCCAATG GAGGCCTGGG CCGCCGCGAG TCGCAGGGCT CCGTGTCCTC TGCGGGGAGT
    CTGGACCTGT CGGATGCCTG TAGGACCTTG ATGCCAGAGC GGGACAAGGC CGCCAAGCAC
    TGCTGCATCC AGCTCCCCGA TGGGACGTCC TGCATGGTGG TCGTCAAGGC AGGCTTATCC
    ATCAAGGAGG TCCTGGCTGG GCTCTGCGAG CGACATGGCA TCAACGGGGC TGCCGTAGAC
    CTCTTCCTGG TGGGCGGGGA CAAGCCTCTG GTGCTGCATC AAGACAGTAG CATCTTGGAG
    TCTCGGGACC TGCGGCTAGA AAAGCGGACT TTGTTTCGGC TGGATCTGGT TCCAATTAAC
    CGGTCAGTGG GACTGAAGGC CAAGCCGACC AAGCCTGTCA CAGAAGTACT GCGGCCCGTG
    GTAGCCAAGT ATGGCCTGCA GCTGAACGAG CTGGTGGCCA GGCTGAGTGG AGAGAAGGAG
    CCCCTGGACC TTGGTGCCCC CATATCAAGT CTGGATGGGC AGAGGGTCAT CTTGGAGGAG
    AAGGAGCCTT CCAGGGGAAA AGGATCCACA GATAAACAGA AAAGTGCACC AGCCAAACAG
    AATATAGCTG TAAATTCCAG TTCCAGAAAT CACTCGGCTA CGGGAGAGGA GAGAACGCTA
    GGCAAGTCTA ATTCTATTAA AATAAAAGGA GAAAATGGAA AAAATGCTAG GGATCCCCGG
    CTTTCAAAGA GAGAAGAATC TATTGCAAAG ATTGGGAAAA AAAAATATCA GAAAATTAAT
    TTGGACGAAG CAGAGGAGTT TTTTGAGCTC ATTTCCAAAG CTCAGAGCAA CAGAGCAGAC
    GACCAGCGCG GGTTGCTGAG GAAGGAGGAC CTGGTGCTAC CCGAGTTTCT GCGCTTACCT
    CCCGGCCCCA TGGAGCTTGC CCTTTCCTCA CCAAGCGCGG CCAAGAGCTT TGGCAAGAGG
    AGCTTGACGG GCAACGGCAA GGAAAAGGCT CCCCAGCCCC AGGAGGCATG GGGGCCCGGC
    CAAGACTTCC GCGAAAGCCC AGCAACAAGC CCCAGCTCGG CTGACAGCAC CCCGTTCAGC
    GCCCCCCTGA CCCCCTCGCC CCCAACACAG GAAGCTGAGG CCGGGGGTGT CCAGACCCTG
    GAGGGCGAAC ACGTGGCCGA CCTGACGCTC ACGGGGGAGG GGGACATGAG CAGCCCCAAC
    AGCACTTTAC TGCCGCCAAC CCCCACCCCG CAGGACTCGG CCGGACCTCC GAGACCAGAC
    ACACCGGGAG TTCATGGGCA CCCTCTGGCA TTTCCTGTCC TGGGAAGGAC CAGCTGGGGA
    GGAGGCGGGG CCAGAGGCAG CCAAGGCCTC CCAGTCAACA CCATCATCGA CGTGGACCGT
    GTGGCTGGCT TGGCACCCGG GCAAGTCAGC AGCATCCCCA GGGTGCGGAC AGGTCCCCAG
    AGGTCACAGG CCAGTGGGCC CCTGACATCA GAGCTCCCCA GCCTGAGCCC CGTCCCAGCT
    GGGCCTACCA AGTTCAAGGC CAGCCAGCAC CATGCAACCT TCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968014.1
    CDS122..4369
    Translation

    Target ORF information:

    RefSeq Version XM_014112539.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112539.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGCT GAGTGGAAAA TCGAAATCAG GAAGATCCCT GAACGAAGAG
    CTGGGGGATG AGGATAGTGA GAAGAAGCGG AAAGGAGCCT TCTTCTCTTG GTCAAGGACC
    AGGAGCACTG GGCGGTCCCA GAAGAAGAAG GAGCATGGTG ACCACACAAA CGAGCCCCTG
    CATGCCAATG GAGGCCTGGG CCGCCGCGAG TCGCAGGGCT CCGTGTCCTC TGCGGGGAGT
    CTGGACCTGT CGGATGCCTG TAGGACCTTG ATGCCAGAGC GGGACAAGGC CGCCAAGCAC
    TGCTGCATCC AGCTCCCCGA TGGGACGTCC TGCATGGTGG TCGTCAAGGC AGGCTTATCC
    ATCAAGGAGG TCCTGGCTGG GCTCTGCGAG CGACATGGCA TCAACGGGGC TGCCGTAGAC
    CTCTTCCTGG TGGGCGGGGA CAAGCCTCTG GTGCTGCATC AAGACAGTAG CATCTTGGAG
    TCTCGGGACC TGCGGCTAGA AAAGCGGACT TTGTTTCGGC TGGATCTGGT TCCAATTAAC
    CGGTCAGTGG GACTGAAGGC CAAGCCGACC AAGCCTGTCA CAGAAGTACT GCGGCCCGTG
    GTAGCCAAGT ATGGCCTGCA GCTGAACGAG CTGGTGGCCA GGCTGAGTGG AGAGAAGGAG
    CCCCTGGACC TTGGTGCCCC CATATCAAGT CTGGATGGGC AGAGGGTCAT CTTGGAGGAG
    AAGGAGCCTT CCAGGGGAAA AGGATCCACA GATAAACAGA AAAGTGCACC AGCCAAACAG
    AATATAGCTG TAAATTCCAG TTCCAGAAAT CACTCGGCTA CGGGAGAGGA GAGAACGCTA
    GGCAAGTCTA ATTCTATTAA AATAAAAGGA GAAAATGGAA AAAATGCTAG GGATCCCCGG
    CTTTCAAAGA GAGAAGAATC TATTGCAAAG ATTGGGAAAA AAAAATATCA GAAAATTAAT
    TTGGACGAAG CAGAGGAGTT TTTTGAGCTC ATTTCCAAAG CTCAGAGCAA CAGAGCAGAC
    GACCAGCGCG GGTTGCTGAG GAAGGAGGAC CTGGTGCTAC CCGAGTTTCT GCGCTTACCT
    CCCGGCCCCA TGGAGCTTGC CCTTTCCTCA CCAAGCGCGG CCAAGAGCTT TGGCAAGAGG
    AGCTTGACGG GCAACGGCAA GGAAAAGGCT CCCCAGCCCC AGGAGGCATG GGGGCCCGGC
    CAAGACTTCC GCGAAAGCCC AGCAACAAGC CCCAGCTCGG CTGACAGCAC CCCGTTCAGC
    GCCCCCCTGA CCCCCTCGCC CCCAACACAG GAAGCTGAGG CCGGGGGTGT CCAGACCCTG
    GAGGGCGAAC ACGTGGCCGA CCTGACGCTC ACGGGGGAGG GGGACATGAG CAGCCCCAAC
    AGCACTTTAC TGCCGCCAAC CCCCACCCCG CAGGACTCGG CCGGACCTCC GAGACCAGAC
    ACACCGGGAG TTCATGGGCA CCCTCTGGCA TTTCCTGTCC TGGGAAGGAC CAGCTGGGGA
    GGAGGCGGGG CCAGAGGCAG CCAAGGCCTC CCAGTCAACA CCATCATCGA CGTGGACCGT
    GTGGCTGGCT TGGCACCCGG GCAAGTCAGC AGCATCCCCA GGGTGCGGAC AGGTCCCCAG
    AGGTCACAGG CCAGTGGGCC CCTGACATCA GAGCTCCCCA GCCTGAGCCC CGTCCCAGCT
    GGGCCTACCA AGTTCAAGGC CAGCCAGCAC CATGCAACCT TCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35800
    Clone ID Related Accession (Same CDS sequence) XM_014112540.2 , XM_014112540.1
    Accession Version XM_014112540.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 12 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCAG CTGAGTGGAA AATCGAAATC AGGAAGATCC
    CTGAACGAAG AGCTGGGGGA TGAGGATAGT GAGAAGAAGC GGAAAGGAGC CTTCTTCTCT
    TGGTCAAGGA CCAGGAGCAC TGGGCGGTCC CAGAAGAAGA AGGAGCATGG TGACCACACA
    AACGAGCCCC TGCATGCCAA TGGAGGCCTG GGCCGCCGCG AGTCGCAGGG CTCCGTGTCC
    TCTGCGGGGA GTCTGGACCT GTCGGATGCC TGTAGGACCT TGATGCCAGA GCGGGACAAG
    GCCGCCAAGC ACTGCTGCAT CCAGCTCCCC GATGGGACGT CCTGCATGGT GGTCGTCAAG
    GCAGGCTTAT CCATCAAGGA GGTCCTGGCT GGGCTCTGCG AGCGACATGG CATCAACGGG
    GCTGCCGTAG ACCTCTTCCT GGTGGGCGGG GACAAGCCTC TGGTGCTGCA TCAAGACAGT
    AGCATCTTGG AGTCTCGGGA CCTGCGGCTA GAAAAGCGGA CTTTGTTTCG GCTGGATCTG
    GTTCCAATTA ACCGGTCAGT GGGACTGAAG GCCAAGCCGA CCAAGCCTGT CACAGAAGTA
    CTGCGGCCCG TGGTAGCCAA GTATGGCCTG CAGCTGAACG AGCTGGTGGC CAGGCTGAGT
    GGAGAGAAGG AGCCCCTGGA CCTTGGTGCC CCCATATCAA GTCTGGATGG GCAGAGGGTC
    ATCTTGGAGG AGAAGGAGCC TTCCAGGGGA AAAGGATCCA CAGATAAACA GAAAAGTGCA
    CCAGCCAAAC AGAATATAGC TGTAAATTCC AGTTCCAGAA ATCACTCGGC TACGAGTTTT
    TTGAGCTCAT TTCCAAAGCT CAGAGCAACA GAGCAGACGA CCAGCGCGGG TTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968015.1
    CDS121..3597
    Translation

    Target ORF information:

    RefSeq Version XM_014112540.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112540.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCAG CTGAGTGGAA AATCGAAATC AGGAAGATCC
    CTGAACGAAG AGCTGGGGGA TGAGGATAGT GAGAAGAAGC GGAAAGGAGC CTTCTTCTCT
    TGGTCAAGGA CCAGGAGCAC TGGGCGGTCC CAGAAGAAGA AGGAGCATGG TGACCACACA
    AACGAGCCCC TGCATGCCAA TGGAGGCCTG GGCCGCCGCG AGTCGCAGGG CTCCGTGTCC
    TCTGCGGGGA GTCTGGACCT GTCGGATGCC TGTAGGACCT TGATGCCAGA GCGGGACAAG
    GCCGCCAAGC ACTGCTGCAT CCAGCTCCCC GATGGGACGT CCTGCATGGT GGTCGTCAAG
    GCAGGCTTAT CCATCAAGGA GGTCCTGGCT GGGCTCTGCG AGCGACATGG CATCAACGGG
    GCTGCCGTAG ACCTCTTCCT GGTGGGCGGG GACAAGCCTC TGGTGCTGCA TCAAGACAGT
    AGCATCTTGG AGTCTCGGGA CCTGCGGCTA GAAAAGCGGA CTTTGTTTCG GCTGGATCTG
    GTTCCAATTA ACCGGTCAGT GGGACTGAAG GCCAAGCCGA CCAAGCCTGT CACAGAAGTA
    CTGCGGCCCG TGGTAGCCAA GTATGGCCTG CAGCTGAACG AGCTGGTGGC CAGGCTGAGT
    GGAGAGAAGG AGCCCCTGGA CCTTGGTGCC CCCATATCAA GTCTGGATGG GCAGAGGGTC
    ATCTTGGAGG AGAAGGAGCC TTCCAGGGGA AAAGGATCCA CAGATAAACA GAAAAGTGCA
    CCAGCCAAAC AGAATATAGC TGTAAATTCC AGTTCCAGAA ATCACTCGGC TACGAGTTTT
    TTGAGCTCAT TTCCAAAGCT CAGAGCAACA GAGCAGACGA CCAGCGCGGG TTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35800
    Clone ID Related Accession (Same CDS sequence) XM_014112540.2 , XM_014112540.1
    Accession Version XM_014112540.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 12 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCAG CTGAGTGGAA AATCGAAATC AGGAAGATCC
    CTGAACGAAG AGCTGGGGGA TGAGGATAGT GAGAAGAAGC GGAAAGGAGC CTTCTTCTCT
    TGGTCAAGGA CCAGGAGCAC TGGGCGGTCC CAGAAGAAGA AGGAGCATGG TGACCACACA
    AACGAGCCCC TGCATGCCAA TGGAGGCCTG GGCCGCCGCG AGTCGCAGGG CTCCGTGTCC
    TCTGCGGGGA GTCTGGACCT GTCGGATGCC TGTAGGACCT TGATGCCAGA GCGGGACAAG
    GCCGCCAAGC ACTGCTGCAT CCAGCTCCCC GATGGGACGT CCTGCATGGT GGTCGTCAAG
    GCAGGCTTAT CCATCAAGGA GGTCCTGGCT GGGCTCTGCG AGCGACATGG CATCAACGGG
    GCTGCCGTAG ACCTCTTCCT GGTGGGCGGG GACAAGCCTC TGGTGCTGCA TCAAGACAGT
    AGCATCTTGG AGTCTCGGGA CCTGCGGCTA GAAAAGCGGA CTTTGTTTCG GCTGGATCTG
    GTTCCAATTA ACCGGTCAGT GGGACTGAAG GCCAAGCCGA CCAAGCCTGT CACAGAAGTA
    CTGCGGCCCG TGGTAGCCAA GTATGGCCTG CAGCTGAACG AGCTGGTGGC CAGGCTGAGT
    GGAGAGAAGG AGCCCCTGGA CCTTGGTGCC CCCATATCAA GTCTGGATGG GCAGAGGGTC
    ATCTTGGAGG AGAAGGAGCC TTCCAGGGGA AAAGGATCCA CAGATAAACA GAAAAGTGCA
    CCAGCCAAAC AGAATATAGC TGTAAATTCC AGTTCCAGAA ATCACTCGGC TACGAGTTTT
    TTGAGCTCAT TTCCAAAGCT CAGAGCAACA GAGCAGACGA CCAGCGCGGG TTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968015.1
    CDS101..3577
    Translation

    Target ORF information:

    RefSeq Version XM_014112540.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCAG CTGAGTGGAA AATCGAAATC AGGAAGATCC
    CTGAACGAAG AGCTGGGGGA TGAGGATAGT GAGAAGAAGC GGAAAGGAGC CTTCTTCTCT
    TGGTCAAGGA CCAGGAGCAC TGGGCGGTCC CAGAAGAAGA AGGAGCATGG TGACCACACA
    AACGAGCCCC TGCATGCCAA TGGAGGCCTG GGCCGCCGCG AGTCGCAGGG CTCCGTGTCC
    TCTGCGGGGA GTCTGGACCT GTCGGATGCC TGTAGGACCT TGATGCCAGA GCGGGACAAG
    GCCGCCAAGC ACTGCTGCAT CCAGCTCCCC GATGGGACGT CCTGCATGGT GGTCGTCAAG
    GCAGGCTTAT CCATCAAGGA GGTCCTGGCT GGGCTCTGCG AGCGACATGG CATCAACGGG
    GCTGCCGTAG ACCTCTTCCT GGTGGGCGGG GACAAGCCTC TGGTGCTGCA TCAAGACAGT
    AGCATCTTGG AGTCTCGGGA CCTGCGGCTA GAAAAGCGGA CTTTGTTTCG GCTGGATCTG
    GTTCCAATTA ACCGGTCAGT GGGACTGAAG GCCAAGCCGA CCAAGCCTGT CACAGAAGTA
    CTGCGGCCCG TGGTAGCCAA GTATGGCCTG CAGCTGAACG AGCTGGTGGC CAGGCTGAGT
    GGAGAGAAGG AGCCCCTGGA CCTTGGTGCC CCCATATCAA GTCTGGATGG GCAGAGGGTC
    ATCTTGGAGG AGAAGGAGCC TTCCAGGGGA AAAGGATCCA CAGATAAACA GAAAAGTGCA
    CCAGCCAAAC AGAATATAGC TGTAAATTCC AGTTCCAGAA ATCACTCGGC TACGAGTTTT
    TTGAGCTCAT TTCCAAAGCT CAGAGCAACA GAGCAGACGA CCAGCGCGGG TTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04433
    Clone ID Related Accession (Same CDS sequence) XM_014112535.2 , XM_014112535.1
    Accession Version XM_014112535.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 12 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCTG AGTGGAAAAT CGAAATCAGG AAGATCCCTG
    AACGAAGAGC TGGGGGATGA GGATAGTGAG AAGAAGCGGA AAGGAGCCTT CTTCTCTTGG
    TCAAGGACCA GGAGCACTGG GCGGTCCCAG AAGAAGAAGG AGCATGGTGA CCACACAAAC
    GAGCCCCTGC ATGCCAATGG AGGCCTGGGC CGCCGCGAGT CGCAGGGCTC CGTGTCCTCT
    GCGGGGAGTC TGGACCTGTC GGATGCCTGT AGGACCTTGA TGCCAGAGCG GGACAAGGCC
    GCCAAGCACT GCTGCATCCA GCTCCCCGAT GGGACGTCCT GCATGGTGGT CGTCAAGGCA
    GGCTTATCCA TCAAGGAGGT CCTGGCTGGG CTCTGCGAGC GACATGGCAT CAACGGGGCT
    GCCGTAGACC TCTTCCTGGT GGGCGGGGAC AAGCCTCTGG TGCTGCATCA AGACAGTAGC
    ATCTTGGAGT CTCGGGACCT GCGGCTAGAA AAGCGGACTT TGTTTCGGCT GGATCTGGTT
    CCAATTAACC GGTCAGTGGG ACTGAAGGCC AAGCCGACCA AGCCTGTCAC AGAAGTACTG
    CGGCCCGTGG TAGCCAAGTA TGGCCTGCAG CTGAACGAGC TGGTGGCCAG GCTGAGTGGA
    GAGAAGGAGC CCCTGGACCT TGGTGCCCCC ATATCAAGTC TGGATGGGCA GAGGGTCATC
    TTGGAGGAGA AGGAGCCTTC CAGGGGAAAA GGATCCACAG ATAAACAGAA AAGTGCACCA
    GCCAAACAGA ATATAGCTGT AAATTCCAGT TCCAGAAATC ACTCGGCTAC GGGAGAGGAG
    AGAACGCTAG GCAAGTCTAA TTCTATTAAA ATAAAAGGAG AAAATGGAAA AAATGCTAGG
    GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG
    AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC
    AGAGCAGACG ACCAGCGCGG GTTGCTGAGG AAGGAGGACC TGGTGCTACC CGAGTTTCTG
    CGCTTACCTC CCGGCCCCAT GGAGCTTGCC CTTTCCTCAC CAAGCGCGGC CAAGAGCTTT
    GGCAAGAGGA GCTTGACGGG CAACGGCAAG GAAAAGGCTC CCCAGCCCCA GGAGGCATGG
    GGGCCCGGCC AAGACTTCCG CGAAAGCCCA GCAACAAGCC CCAGCTCGGC TGACAGCACC
    CCGTTCAGCG CCCCCCTGAC CCCCTCGCCC CCAACACAGG AAGCTGAGGC CGGGGGTGTC
    CAGACCCTGG AGGGCGAACA CGTGGCCGAC CTGACGCTCA CGGGGGAGGG GGACATGAGC
    AGCCCCAACA GCACTTTACT GCCGCCAACC CCCACCCCGC AGGACTCGGC CGGACCTCCG
    AGACCAGACA CACCGGGAGT TCATGGGCAC CCTCTGGCAT TTCCTGTCCT GGGAAGGACC
    AGCTGGGGAG GAGGCGGGGC CAGAGGCAGC CAAGGCCTCC CAGTCAACAC CATCATCGAC
    GTGGACCGTG TGGCTGGCTT GGCACCCGGG CAAGTCAGCA GCATCCCCAG GGTGCGGACA
    GGTCCCCAGA GGTCACAGGC CAGTGGGCCC CTGACATCAG AGCTCCCCAG CCTGAGCCCC
    GTCCCAGCTG GGCCTACCAA GTTCAAGGCC AGCCAGCACC ATGCAACCTT CGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968010.1
    CDS121..4437
    Translation

    Target ORF information:

    RefSeq Version XM_014112535.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112535.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCTG AGTGGAAAAT CGAAATCAGG AAGATCCCTG
    AACGAAGAGC TGGGGGATGA GGATAGTGAG AAGAAGCGGA AAGGAGCCTT CTTCTCTTGG
    TCAAGGACCA GGAGCACTGG GCGGTCCCAG AAGAAGAAGG AGCATGGTGA CCACACAAAC
    GAGCCCCTGC ATGCCAATGG AGGCCTGGGC CGCCGCGAGT CGCAGGGCTC CGTGTCCTCT
    GCGGGGAGTC TGGACCTGTC GGATGCCTGT AGGACCTTGA TGCCAGAGCG GGACAAGGCC
    GCCAAGCACT GCTGCATCCA GCTCCCCGAT GGGACGTCCT GCATGGTGGT CGTCAAGGCA
    GGCTTATCCA TCAAGGAGGT CCTGGCTGGG CTCTGCGAGC GACATGGCAT CAACGGGGCT
    GCCGTAGACC TCTTCCTGGT GGGCGGGGAC AAGCCTCTGG TGCTGCATCA AGACAGTAGC
    ATCTTGGAGT CTCGGGACCT GCGGCTAGAA AAGCGGACTT TGTTTCGGCT GGATCTGGTT
    CCAATTAACC GGTCAGTGGG ACTGAAGGCC AAGCCGACCA AGCCTGTCAC AGAAGTACTG
    CGGCCCGTGG TAGCCAAGTA TGGCCTGCAG CTGAACGAGC TGGTGGCCAG GCTGAGTGGA
    GAGAAGGAGC CCCTGGACCT TGGTGCCCCC ATATCAAGTC TGGATGGGCA GAGGGTCATC
    TTGGAGGAGA AGGAGCCTTC CAGGGGAAAA GGATCCACAG ATAAACAGAA AAGTGCACCA
    GCCAAACAGA ATATAGCTGT AAATTCCAGT TCCAGAAATC ACTCGGCTAC GGGAGAGGAG
    AGAACGCTAG GCAAGTCTAA TTCTATTAAA ATAAAAGGAG AAAATGGAAA AAATGCTAGG
    GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG
    AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC
    AGAGCAGACG ACCAGCGCGG GTTGCTGAGG AAGGAGGACC TGGTGCTACC CGAGTTTCTG
    CGCTTACCTC CCGGCCCCAT GGAGCTTGCC CTTTCCTCAC CAAGCGCGGC CAAGAGCTTT
    GGCAAGAGGA GCTTGACGGG CAACGGCAAG GAAAAGGCTC CCCAGCCCCA GGAGGCATGG
    GGGCCCGGCC AAGACTTCCG CGAAAGCCCA GCAACAAGCC CCAGCTCGGC TGACAGCACC
    CCGTTCAGCG CCCCCCTGAC CCCCTCGCCC CCAACACAGG AAGCTGAGGC CGGGGGTGTC
    CAGACCCTGG AGGGCGAACA CGTGGCCGAC CTGACGCTCA CGGGGGAGGG GGACATGAGC
    AGCCCCAACA GCACTTTACT GCCGCCAACC CCCACCCCGC AGGACTCGGC CGGACCTCCG
    AGACCAGACA CACCGGGAGT TCATGGGCAC CCTCTGGCAT TTCCTGTCCT GGGAAGGACC
    AGCTGGGGAG GAGGCGGGGC CAGAGGCAGC CAAGGCCTCC CAGTCAACAC CATCATCGAC
    GTGGACCGTG TGGCTGGCTT GGCACCCGGG CAAGTCAGCA GCATCCCCAG GGTGCGGACA
    GGTCCCCAGA GGTCACAGGC CAGTGGGCCC CTGACATCAG AGCTCCCCAG CCTGAGCCCC
    GTCCCAGCTG GGCCTACCAA GTTCAAGGCC AGCCAGCACC ATGCAACCTT CGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04433
    Clone ID Related Accession (Same CDS sequence) XM_014112535.2 , XM_014112535.1
    Accession Version XM_014112535.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 12 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCTG AGTGGAAAAT CGAAATCAGG AAGATCCCTG
    AACGAAGAGC TGGGGGATGA GGATAGTGAG AAGAAGCGGA AAGGAGCCTT CTTCTCTTGG
    TCAAGGACCA GGAGCACTGG GCGGTCCCAG AAGAAGAAGG AGCATGGTGA CCACACAAAC
    GAGCCCCTGC ATGCCAATGG AGGCCTGGGC CGCCGCGAGT CGCAGGGCTC CGTGTCCTCT
    GCGGGGAGTC TGGACCTGTC GGATGCCTGT AGGACCTTGA TGCCAGAGCG GGACAAGGCC
    GCCAAGCACT GCTGCATCCA GCTCCCCGAT GGGACGTCCT GCATGGTGGT CGTCAAGGCA
    GGCTTATCCA TCAAGGAGGT CCTGGCTGGG CTCTGCGAGC GACATGGCAT CAACGGGGCT
    GCCGTAGACC TCTTCCTGGT GGGCGGGGAC AAGCCTCTGG TGCTGCATCA AGACAGTAGC
    ATCTTGGAGT CTCGGGACCT GCGGCTAGAA AAGCGGACTT TGTTTCGGCT GGATCTGGTT
    CCAATTAACC GGTCAGTGGG ACTGAAGGCC AAGCCGACCA AGCCTGTCAC AGAAGTACTG
    CGGCCCGTGG TAGCCAAGTA TGGCCTGCAG CTGAACGAGC TGGTGGCCAG GCTGAGTGGA
    GAGAAGGAGC CCCTGGACCT TGGTGCCCCC ATATCAAGTC TGGATGGGCA GAGGGTCATC
    TTGGAGGAGA AGGAGCCTTC CAGGGGAAAA GGATCCACAG ATAAACAGAA AAGTGCACCA
    GCCAAACAGA ATATAGCTGT AAATTCCAGT TCCAGAAATC ACTCGGCTAC GGGAGAGGAG
    AGAACGCTAG GCAAGTCTAA TTCTATTAAA ATAAAAGGAG AAAATGGAAA AAATGCTAGG
    GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG
    AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC
    AGAGCAGACG ACCAGCGCGG GTTGCTGAGG AAGGAGGACC TGGTGCTACC CGAGTTTCTG
    CGCTTACCTC CCGGCCCCAT GGAGCTTGCC CTTTCCTCAC CAAGCGCGGC CAAGAGCTTT
    GGCAAGAGGA GCTTGACGGG CAACGGCAAG GAAAAGGCTC CCCAGCCCCA GGAGGCATGG
    GGGCCCGGCC AAGACTTCCG CGAAAGCCCA GCAACAAGCC CCAGCTCGGC TGACAGCACC
    CCGTTCAGCG CCCCCCTGAC CCCCTCGCCC CCAACACAGG AAGCTGAGGC CGGGGGTGTC
    CAGACCCTGG AGGGCGAACA CGTGGCCGAC CTGACGCTCA CGGGGGAGGG GGACATGAGC
    AGCCCCAACA GCACTTTACT GCCGCCAACC CCCACCCCGC AGGACTCGGC CGGACCTCCG
    AGACCAGACA CACCGGGAGT TCATGGGCAC CCTCTGGCAT TTCCTGTCCT GGGAAGGACC
    AGCTGGGGAG GAGGCGGGGC CAGAGGCAGC CAAGGCCTCC CAGTCAACAC CATCATCGAC
    GTGGACCGTG TGGCTGGCTT GGCACCCGGG CAAGTCAGCA GCATCCCCAG GGTGCGGACA
    GGTCCCCAGA GGTCACAGGC CAGTGGGCCC CTGACATCAG AGCTCCCCAG CCTGAGCCCC
    GTCCCAGCTG GGCCTACCAA GTTCAAGGCC AGCCAGCACC ATGCAACCTT CGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968010.1
    CDS101..4417
    Translation

    Target ORF information:

    RefSeq Version XM_014112535.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCTG AGTGGAAAAT CGAAATCAGG AAGATCCCTG
    AACGAAGAGC TGGGGGATGA GGATAGTGAG AAGAAGCGGA AAGGAGCCTT CTTCTCTTGG
    TCAAGGACCA GGAGCACTGG GCGGTCCCAG AAGAAGAAGG AGCATGGTGA CCACACAAAC
    GAGCCCCTGC ATGCCAATGG AGGCCTGGGC CGCCGCGAGT CGCAGGGCTC CGTGTCCTCT
    GCGGGGAGTC TGGACCTGTC GGATGCCTGT AGGACCTTGA TGCCAGAGCG GGACAAGGCC
    GCCAAGCACT GCTGCATCCA GCTCCCCGAT GGGACGTCCT GCATGGTGGT CGTCAAGGCA
    GGCTTATCCA TCAAGGAGGT CCTGGCTGGG CTCTGCGAGC GACATGGCAT CAACGGGGCT
    GCCGTAGACC TCTTCCTGGT GGGCGGGGAC AAGCCTCTGG TGCTGCATCA AGACAGTAGC
    ATCTTGGAGT CTCGGGACCT GCGGCTAGAA AAGCGGACTT TGTTTCGGCT GGATCTGGTT
    CCAATTAACC GGTCAGTGGG ACTGAAGGCC AAGCCGACCA AGCCTGTCAC AGAAGTACTG
    CGGCCCGTGG TAGCCAAGTA TGGCCTGCAG CTGAACGAGC TGGTGGCCAG GCTGAGTGGA
    GAGAAGGAGC CCCTGGACCT TGGTGCCCCC ATATCAAGTC TGGATGGGCA GAGGGTCATC
    TTGGAGGAGA AGGAGCCTTC CAGGGGAAAA GGATCCACAG ATAAACAGAA AAGTGCACCA
    GCCAAACAGA ATATAGCTGT AAATTCCAGT TCCAGAAATC ACTCGGCTAC GGGAGAGGAG
    AGAACGCTAG GCAAGTCTAA TTCTATTAAA ATAAAAGGAG AAAATGGAAA AAATGCTAGG
    GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG
    AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC
    AGAGCAGACG ACCAGCGCGG GTTGCTGAGG AAGGAGGACC TGGTGCTACC CGAGTTTCTG
    CGCTTACCTC CCGGCCCCAT GGAGCTTGCC CTTTCCTCAC CAAGCGCGGC CAAGAGCTTT
    GGCAAGAGGA GCTTGACGGG CAACGGCAAG GAAAAGGCTC CCCAGCCCCA GGAGGCATGG
    GGGCCCGGCC AAGACTTCCG CGAAAGCCCA GCAACAAGCC CCAGCTCGGC TGACAGCACC
    CCGTTCAGCG CCCCCCTGAC CCCCTCGCCC CCAACACAGG AAGCTGAGGC CGGGGGTGTC
    CAGACCCTGG AGGGCGAACA CGTGGCCGAC CTGACGCTCA CGGGGGAGGG GGACATGAGC
    AGCCCCAACA GCACTTTACT GCCGCCAACC CCCACCCCGC AGGACTCGGC CGGACCTCCG
    AGACCAGACA CACCGGGAGT TCATGGGCAC CCTCTGGCAT TTCCTGTCCT GGGAAGGACC
    AGCTGGGGAG GAGGCGGGGC CAGAGGCAGC CAAGGCCTCC CAGTCAACAC CATCATCGAC
    GTGGACCGTG TGGCTGGCTT GGCACCCGGG CAAGTCAGCA GCATCCCCAG GGTGCGGACA
    GGTCCCCAGA GGTCACAGGC CAGTGGGCCC CTGACATCAG AGCTCCCCAG CCTGAGCCCC
    GTCCCAGCTG GGCCTACCAA GTTCAAGGCC AGCCAGCACC ATGCAACCTT CGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04434
    Clone ID Related Accession (Same CDS sequence) XM_014112537.1 , XM_014112537.2
    Accession Version XM_014112537.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product regulator of G-protein signaling 12 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCTG AGTGGAAAAT CGAAATCAGG AAGATCCCTG
    AACGAAGAGC TGGGGGATGA GGATAGTGAG AAGAAGCGGA AAGGAGCCTT CTTCTCTTGG
    TCAAGGACCA GGAGCACTGG GCGGTCCCAG AAGAAGAAGG AGCATGGTGA CCACACAAAC
    GAGCCCCTGC ATGCCAATGG AGGCCTGGGC CGCCGCGAGT CGCAGGGCTC CGTGTCCTCT
    GCGGGGAGTC TGGACCTGTC GGATGCCTGT AGGACCTTGA TGCCAGAGCG GGACAAGGCC
    GCCAAGCACT GCTGCATCCA GCTCCCCGAT GGGACGTCCT GCATGGTGGT CGTCAAGGCA
    GGCTTATCCA TCAAGGAGGT CCTGGCTGGG CTCTGCGAGC GACATGGCAT CAACGGGGCT
    GCCGTAGACC TCTTCCTGGT GGGCGGGGAC AAGCCTCTGG TGCTGCATCA AGACAGTAGC
    ATCTTGGAGT CTCGGGACCT GCGGCTAGAA AAGCGGACTT TGTTTCGGCT GGATCTGGTT
    CCAATTAACC GGTCAGTGGG ACTGAAGGCC AAGCCGACCA AGCCTGTCAC AGAAGTACTG
    CGGCCCGTGG TAGCCAAGTA TGGCCTGCAG CTGAACGAGC TGGTGGCCAG GCTGAGTGGA
    GAGAAGGAGC CCCTGGACCT TGGTGCCCCC ATATCAAGTC TGGATGGGCA GAGGGTCATC
    TTGGAGGAGA AGGAGCCTTC CAGGGGAAAA GGATCCACAG ATAAACAGAA AAGTGCACCA
    GCCAAACAGA ATATAGCTGT AAATTCCAGT TCCAGAAATC ACTCGGCTAC GGGAGAGGAG
    AGAACGCTAG GCAAGTCTAA TTCTATTAAA ATAAAAGGAG AAAATGGAAA AAATGCTAGG
    GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG
    AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC
    AGAGCAGACG ACCAGCGCGG GTTGCTGAGG AAGGAGGACC TGGTGCTACC CGAGTTTCTG
    CGCTTACCTC CCGGCCCCAT GGAGCTTGCC CTTTCCTCAC CAAGCGCGGC CAAGAGCTTT
    GGCAAGAGGA GCTTGACGGG CAACGGCAAG GAAAAGGCTC CCCAGCCCCA GGAGGCATGG
    GGGCCCGGCC AAGACTTCCG CGAAAGCCCA GCAACAAGCC CCAGCTCGGC TGACAGCACC
    CCGTTCAGCG CCCCCCTGAC CCCCTCGCCC CCAACACAGG AAGCTGAGGC CGGGGGTGTC
    CAGACCCTGG AGGGCGAACA CGTGGCCGAC CTGACGCTCA CGGGGGAGGG GGACATGAGC
    AGCCCCAACA GCACTTTACT GCCGCCAACC CCCACCCCGC AGGACTCGGC CGGACCTCCG
    AGACCAGGAG GCGGGGCCAG AGGCAGCCAA GGCCTCCCAG TCAACACCAT CATCGACGTG
    GACCGTGTGG CTGGCTTGGC ACCCGGGCAA GTCAGCAGCA TCCCCAGGGT GCGGACAGGT
    CCCCAGAGGT CACAGGCCAG TGGGCCCCTG ACATCAGAGC TCCCCAGCCT GAGCCCCGTC
    CCAGCTGGGC CTACCAAGTT CAAGGCCAGC CAGCACCATG CAACCTTCGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968012.1
    CDS101..4354
    Translation

    Target ORF information:

    RefSeq Version XM_014112537.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris regulator of G-protein signaling 12 (RGS12), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCTG AGTGGAAAAT CGAAATCAGG AAGATCCCTG
    AACGAAGAGC TGGGGGATGA GGATAGTGAG AAGAAGCGGA AAGGAGCCTT CTTCTCTTGG
    TCAAGGACCA GGAGCACTGG GCGGTCCCAG AAGAAGAAGG AGCATGGTGA CCACACAAAC
    GAGCCCCTGC ATGCCAATGG AGGCCTGGGC CGCCGCGAGT CGCAGGGCTC CGTGTCCTCT
    GCGGGGAGTC TGGACCTGTC GGATGCCTGT AGGACCTTGA TGCCAGAGCG GGACAAGGCC
    GCCAAGCACT GCTGCATCCA GCTCCCCGAT GGGACGTCCT GCATGGTGGT CGTCAAGGCA
    GGCTTATCCA TCAAGGAGGT CCTGGCTGGG CTCTGCGAGC GACATGGCAT CAACGGGGCT
    GCCGTAGACC TCTTCCTGGT GGGCGGGGAC AAGCCTCTGG TGCTGCATCA AGACAGTAGC
    ATCTTGGAGT CTCGGGACCT GCGGCTAGAA AAGCGGACTT TGTTTCGGCT GGATCTGGTT
    CCAATTAACC GGTCAGTGGG ACTGAAGGCC AAGCCGACCA AGCCTGTCAC AGAAGTACTG
    CGGCCCGTGG TAGCCAAGTA TGGCCTGCAG CTGAACGAGC TGGTGGCCAG GCTGAGTGGA
    GAGAAGGAGC CCCTGGACCT TGGTGCCCCC ATATCAAGTC TGGATGGGCA GAGGGTCATC
    TTGGAGGAGA AGGAGCCTTC CAGGGGAAAA GGATCCACAG ATAAACAGAA AAGTGCACCA
    GCCAAACAGA ATATAGCTGT AAATTCCAGT TCCAGAAATC ACTCGGCTAC GGGAGAGGAG
    AGAACGCTAG GCAAGTCTAA TTCTATTAAA ATAAAAGGAG AAAATGGAAA AAATGCTAGG
    GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG
    AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC
    AGAGCAGACG ACCAGCGCGG GTTGCTGAGG AAGGAGGACC TGGTGCTACC CGAGTTTCTG
    CGCTTACCTC CCGGCCCCAT GGAGCTTGCC CTTTCCTCAC CAAGCGCGGC CAAGAGCTTT
    GGCAAGAGGA GCTTGACGGG CAACGGCAAG GAAAAGGCTC CCCAGCCCCA GGAGGCATGG
    GGGCCCGGCC AAGACTTCCG CGAAAGCCCA GCAACAAGCC CCAGCTCGGC TGACAGCACC
    CCGTTCAGCG CCCCCCTGAC CCCCTCGCCC CCAACACAGG AAGCTGAGGC CGGGGGTGTC
    CAGACCCTGG AGGGCGAACA CGTGGCCGAC CTGACGCTCA CGGGGGAGGG GGACATGAGC
    AGCCCCAACA GCACTTTACT GCCGCCAACC CCCACCCCGC AGGACTCGGC CGGACCTCCG
    AGACCAGGAG GCGGGGCCAG AGGCAGCCAA GGCCTCCCAG TCAACACCAT CATCGACGTG
    GACCGTGTGG CTGGCTTGGC ACCCGGGCAA GTCAGCAGCA TCCCCAGGGT GCGGACAGGT
    CCCCAGAGGT CACAGGCCAG TGGGCCCCTG ACATCAGAGC TCCCCAGCCT GAGCCCCGTC
    CCAGCTGGGC CTACCAAGTT CAAGGCCAGC CAGCACCATG CAACCTTCGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04434
    Clone ID Related Accession (Same CDS sequence) XM_014112537.1 , XM_014112537.2
    Accession Version XM_014112537.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product regulator of G-protein signaling 12 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCTG AGTGGAAAAT CGAAATCAGG AAGATCCCTG
    AACGAAGAGC TGGGGGATGA GGATAGTGAG AAGAAGCGGA AAGGAGCCTT CTTCTCTTGG
    TCAAGGACCA GGAGCACTGG GCGGTCCCAG AAGAAGAAGG AGCATGGTGA CCACACAAAC
    GAGCCCCTGC ATGCCAATGG AGGCCTGGGC CGCCGCGAGT CGCAGGGCTC CGTGTCCTCT
    GCGGGGAGTC TGGACCTGTC GGATGCCTGT AGGACCTTGA TGCCAGAGCG GGACAAGGCC
    GCCAAGCACT GCTGCATCCA GCTCCCCGAT GGGACGTCCT GCATGGTGGT CGTCAAGGCA
    GGCTTATCCA TCAAGGAGGT CCTGGCTGGG CTCTGCGAGC GACATGGCAT CAACGGGGCT
    GCCGTAGACC TCTTCCTGGT GGGCGGGGAC AAGCCTCTGG TGCTGCATCA AGACAGTAGC
    ATCTTGGAGT CTCGGGACCT GCGGCTAGAA AAGCGGACTT TGTTTCGGCT GGATCTGGTT
    CCAATTAACC GGTCAGTGGG ACTGAAGGCC AAGCCGACCA AGCCTGTCAC AGAAGTACTG
    CGGCCCGTGG TAGCCAAGTA TGGCCTGCAG CTGAACGAGC TGGTGGCCAG GCTGAGTGGA
    GAGAAGGAGC CCCTGGACCT TGGTGCCCCC ATATCAAGTC TGGATGGGCA GAGGGTCATC
    TTGGAGGAGA AGGAGCCTTC CAGGGGAAAA GGATCCACAG ATAAACAGAA AAGTGCACCA
    GCCAAACAGA ATATAGCTGT AAATTCCAGT TCCAGAAATC ACTCGGCTAC GGGAGAGGAG
    AGAACGCTAG GCAAGTCTAA TTCTATTAAA ATAAAAGGAG AAAATGGAAA AAATGCTAGG
    GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG
    AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC
    AGAGCAGACG ACCAGCGCGG GTTGCTGAGG AAGGAGGACC TGGTGCTACC CGAGTTTCTG
    CGCTTACCTC CCGGCCCCAT GGAGCTTGCC CTTTCCTCAC CAAGCGCGGC CAAGAGCTTT
    GGCAAGAGGA GCTTGACGGG CAACGGCAAG GAAAAGGCTC CCCAGCCCCA GGAGGCATGG
    GGGCCCGGCC AAGACTTCCG CGAAAGCCCA GCAACAAGCC CCAGCTCGGC TGACAGCACC
    CCGTTCAGCG CCCCCCTGAC CCCCTCGCCC CCAACACAGG AAGCTGAGGC CGGGGGTGTC
    CAGACCCTGG AGGGCGAACA CGTGGCCGAC CTGACGCTCA CGGGGGAGGG GGACATGAGC
    AGCCCCAACA GCACTTTACT GCCGCCAACC CCCACCCCGC AGGACTCGGC CGGACCTCCG
    AGACCAGGAG GCGGGGCCAG AGGCAGCCAA GGCCTCCCAG TCAACACCAT CATCGACGTG
    GACCGTGTGG CTGGCTTGGC ACCCGGGCAA GTCAGCAGCA TCCCCAGGGT GCGGACAGGT
    CCCCAGAGGT CACAGGCCAG TGGGCCCCTG ACATCAGAGC TCCCCAGCCT GAGCCCCGTC
    CCAGCTGGGC CTACCAAGTT CAAGGCCAGC CAGCACCATG CAACCTTCGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968012.1
    CDS122..4375
    Translation

    Target ORF information:

    RefSeq Version XM_014112537.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris regulator of G protein signaling 12 (RGS12), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112537.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTATAGAG CAGGGGACAC AGTGGAGCGT CCACTTCATG GGCCGGCGCC CCTTCGCATG 
    AGGAGTGTGG AAGTGGCCAG AGGAAGAACA GGGTACGGAT TCACCCTTTC TGGTCAGGCG
    CCTTGTGTGC TCAGCTGTGT CATGAGAGGG AGCCCTGCAG ATTTTGTGGG CCTCCGTGCT
    GGTGATCAGA TATTTGCTGT CAATGAAATC AATGTGAAGA AAGCGTCCCA TGAGGATGTA
    GTGAAATTAA TTGGGAAATG TTCTGGTGTC CTGCACATGG TGATTGGTGA AGGCATTGGC
    CACGTGGAGT CCTGTTCCAG TGATGAAGAG ATTGGATTTT ATGAAGGAAA AGGCTGGCTG
    AAGCCCAAGC TTGATTCTAA AGCCTTAGGT ATAAATAGAG CAGAGCGAGT TGTTGAAGAA
    ATGCAGTCCG GTGGAATTCT CAGTATGATC TTTGAAAATC CTAGTCTTCG TGCGAGCAAT
    TCAGAGCCCT TGAAATTGAA ACAAAGATCC CTTTCAGAGT CAGCCGCTGC GCGATTTGAC
    CTTGGACGTG AAAGTATAAG TCATCCGAAT CCTGGCCTGC TTTCTAAGGA GGAAGTATCA
    AAAATGATTA ATGATGATTC GGTTTTCAGT ATCGGACCTG AGAATCCTGA AGATTTTGTA
    GTAGATGCAA GTATTTTAAA CGTTGCCATG GTCGTAGGCT ACTTAGGTTC CATCGAACTT
    CCTTCCACAA GCTGCAACCT TGAGTCGGAC AGTTTACAGG CGATCCGTGG CTGCCTGCGG
    CGCTTGCGGG CGGAGCAGAA AATCCACTCC CTGGTGACGA TGCGGGTCCT GCACGACCGT
    GTGCAGCTCT GTACTGACCA GGCGGCTGTT CTGGCCGAGT ACCCGGCTGA GAAGCTGGCC
    TTCAGTGCTG TGTGCCCAGA TGACAGGAGG CTCTTTGGGC TCGTGACCAT GCAGACCAGT
    GACGATGGGA GTCTGGCTCA CGAGGATGAG GGTGCCTTGC GGACTTCCTG CCATGTGTTC
    ATGGTGGATC CAGACTTATT CAATCACAAG ATCCATCAGG GCATTGCTCG GCGGTTTGGG
    TTTGAGTGCA CGGCTGACCC CGACACCAAC GGCTGCCTTG AGTTCCCAGC ATCCTCTCTC
    CCTGTCCTCC AGTTTGTCTC CGTACTGTAC AGAGACATGG GCGAGCTAAT CGAGGGAGTC
    AGGGCCCGGG CCTTTGTGGA CGGGGATGCG GATGCCCACC AGAACACCAG CACCAGCAGC
    AACAGTGACA GTGGCATTGG CAACTTCCAC CAGGAGGAGC GAAGCAGCCG AGTGCTTGTG
    GTTGACCTGG GCGCCAGCTC TAGCAGGCAC GGCCCCAGCA ACAGTGGCTG GGAGGGTGCC
    GGTGGGAGGA GCTCCCAGCC TTGGGGTGCC CCCTGGAATG GGGCCTTTTG CCACGACCCC
    GAAGGGGGCC CCCTGTTTGA GGCCTCTCCA CAGGCTGATA GGTGCCGGGA CTTCAGTAAG
    CACTTAGGGT CCACTTCTCA CGGAGAGGCT CCCCCTGCCC CCTTGCGGAG TTCAGTGCCG
    CCTTCCAAGA GGGGTGCCGT GGGCCATGGC GGTAGTTTCG GCCAGCGGTG GCTTCCAGTC
    CACGTCCTGC AGGAGTGGCA GTGCGGACAT GCCAGTGACC AGGAGTCTTA CACAGACTCC
    ACCGATGGGT GGTCTAGTGT CAACTGCGGC ACGCTGCCCC CTCCCATGAG CAAGATTCCC
    GCCGACCGCT ACAGGGTGGA GGGCAGCTTG GCACAGCCCC CCCTGAGCAC CCAGAAAAGG
    GACTGGTCCA GGAAGGCTTT CGGGATGCAA AACATTTTCG GTCCCCATCG ACAAGTTAGA
    AAGACTAAGG AAGACAAAAA GGGCTCAAAA TTTGGGCGGG GAATCGGACT CACTCAGACT
    TCTCAACGCA CCTCTGCCCG GAGATCATTT GGAAGATCCA AGAGATTTAG TATCACTCGC
    TCCCTTGATG ATCTCGAGTC TGCAACTGTG TCTGATGGCG AGCTGACCGG CACTGACCTG
    AAGGACTGCG TGAGCAACCA CAGCCTGAGC AGCAATGCCA GCCTCCCCAG CGTGCAGAGC
    TGCAGGCGCC TGCGTGAGAG GAGGGCCGCC AGCTGGGCCG TGTCATTCGA GCGCCTTCTG
    CAGGACCCTC TCGGTGTTCG ATACTTCTCT GATTTCCTAA GAAAGGAATT CAGTGAAGAA
    AACATTTTAT TCTGGCAGGC CTGTGAATAT TTTAATCATG TTCCTGCACA CGATAAAAAA
    GAGCTTTCCT ATAGGGCCCG CGAGATCTTT AGCAAGTTCC TGTGCAGCAA AGCCACCACC
    CCCGTCAACA TTGACAGCCA GGCCCAGCTG GCAGATGACA TTCTCAGTGC TCCTCACCCC
    GACATGTTCA AGGAGCAGCA GCTGCAGATC TTCAACCTGA TGAAGTTTGA TAGCTACACT
    CGCTTTCTCA AGTCCCAGCT GTACCAGGAG TGCATCCTGG CCGAAGTGGA AGGCCGCTCC
    CTCCCTGACG CACAGCAGGT ACCCAGCAGC CCGGCCTCCA AGCACAGCGT CAGCTCAGAG
    CACTCCAGCG TGTCCACGCC AAAAAAGCTG AGTGGAAAAT CGAAATCAGG AAGATCCCTG
    AACGAAGAGC TGGGGGATGA GGATAGTGAG AAGAAGCGGA AAGGAGCCTT CTTCTCTTGG
    TCAAGGACCA GGAGCACTGG GCGGTCCCAG AAGAAGAAGG AGCATGGTGA CCACACAAAC
    GAGCCCCTGC ATGCCAATGG AGGCCTGGGC CGCCGCGAGT CGCAGGGCTC CGTGTCCTCT
    GCGGGGAGTC TGGACCTGTC GGATGCCTGT AGGACCTTGA TGCCAGAGCG GGACAAGGCC
    GCCAAGCACT GCTGCATCCA GCTCCCCGAT GGGACGTCCT GCATGGTGGT CGTCAAGGCA
    GGCTTATCCA TCAAGGAGGT CCTGGCTGGG CTCTGCGAGC GACATGGCAT CAACGGGGCT
    GCCGTAGACC TCTTCCTGGT GGGCGGGGAC AAGCCTCTGG TGCTGCATCA AGACAGTAGC
    ATCTTGGAGT CTCGGGACCT GCGGCTAGAA AAGCGGACTT TGTTTCGGCT GGATCTGGTT
    CCAATTAACC GGTCAGTGGG ACTGAAGGCC AAGCCGACCA AGCCTGTCAC AGAAGTACTG
    CGGCCCGTGG TAGCCAAGTA TGGCCTGCAG CTGAACGAGC TGGTGGCCAG GCTGAGTGGA
    GAGAAGGAGC CCCTGGACCT TGGTGCCCCC ATATCAAGTC TGGATGGGCA GAGGGTCATC
    TTGGAGGAGA AGGAGCCTTC CAGGGGAAAA GGATCCACAG ATAAACAGAA AAGTGCACCA
    GCCAAACAGA ATATAGCTGT AAATTCCAGT TCCAGAAATC ACTCGGCTAC GGGAGAGGAG
    AGAACGCTAG GCAAGTCTAA TTCTATTAAA ATAAAAGGAG AAAATGGAAA AAATGCTAGG
    GATCCCCGGC TTTCAAAGAG AGAAGAATCT ATTGCAAAGA TTGGGAAAAA AAAATATCAG
    AAAATTAATT TGGACGAAGC AGAGGAGTTT TTTGAGCTCA TTTCCAAAGC TCAGAGCAAC
    AGAGCAGACG ACCAGCGCGG GTTGCTGAGG AAGGAGGACC TGGTGCTACC CGAGTTTCTG
    CGCTTACCTC CCGGCCCCAT GGAGCTTGCC CTTTCCTCAC CAAGCGCGGC CAAGAGCTTT
    GGCAAGAGGA GCTTGACGGG CAACGGCAAG GAAAAGGCTC CCCAGCCCCA GGAGGCATGG
    GGGCCCGGCC AAGACTTCCG CGAAAGCCCA GCAACAAGCC CCAGCTCGGC TGACAGCACC
    CCGTTCAGCG CCCCCCTGAC CCCCTCGCCC CCAACACAGG AAGCTGAGGC CGGGGGTGTC
    CAGACCCTGG AGGGCGAACA CGTGGCCGAC CTGACGCTCA CGGGGGAGGG GGACATGAGC
    AGCCCCAACA GCACTTTACT GCCGCCAACC CCCACCCCGC AGGACTCGGC CGGACCTCCG
    AGACCAGGAG GCGGGGCCAG AGGCAGCCAA GGCCTCCCAG TCAACACCAT CATCGACGTG
    GACCGTGTGG CTGGCTTGGC ACCCGGGCAA GTCAGCAGCA TCCCCAGGGT GCGGACAGGT
    CCCCAGAGGT CACAGGCCAG TGGGCCCCTG ACATCAGAGC TCCCCAGCCT GAGCCCCGTC
    CCAGCTGGGC CTACCAAGTT CAAGGCCAGC CAGCACCATG CAACCTTCGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.