scrib cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	scrib
Entrez Gene ID	368473
Full Name	scribbled planar cell polarity protein
Synonyms	scrb1,scribble,scribble1,si:ch211-12p8.1,si:zc12p8.1
General protein information	Preferred Names scribbled planar cell polarity protein Names protein scribble homolog landlocked llk
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021477417.1	XP_021333092.1	protein scribble homolog isoform X4
XM_021477418.1	XP_021333093.1	protein scribble homolog isoform X5
XM_021477425.1	XP_021333100.1	protein scribble homolog isoform X11
XM_021477429.1	XP_021333104.1	protein scribble homolog isoform X15
XM_009303383.2	XP_009301658.1	protein scribble homolog isoform X1
XM_009303392.2	XP_009301667.1	protein scribble homolog isoform X10
NM_001007175.1	NP_001007176.1	protein scribble homolog
XM_009303388.2	XP_009301663.1	protein scribble homolog isoform X6
XM_021477414.1	XP_021333089.1	protein scribble homolog isoform X1
XM_021477422.1	XP_021333097.1	protein scribble homolog isoform X8
XM_021477426.1	XP_021333101.1	protein scribble homolog isoform X12
XM_021477433.1	XP_021333108.1	protein scribble homolog isoform X19
XM_009303386.2	XP_009301661.1	protein scribble homolog isoform X4
XM_009303394.2	XP_009301669.1	protein scribble homolog isoform X12
XM_021477416.1	XP_021333091.1	protein scribble homolog isoform X3
XM_021477419.1	XP_021333094.1	protein scribble homolog isoform X6
XM_021477421.1	XP_021333096.1	protein scribble homolog isoform X7
XM_009303384.2	XP_009301659.1	protein scribble homolog isoform X2
XM_009303387.2	XP_009301662.1	protein scribble homolog isoform X5
XM_009303390.2	XP_009301665.1	protein scribble homolog isoform X8
XM_021477424.1	XP_021333099.1	protein scribble homolog isoform X10
XM_021477427.1	XP_021333102.1	protein scribble homolog isoform X13
XM_021477432.1	XP_021333107.1	protein scribble homolog isoform X18
XM_009303385.2	XP_009301660.1	protein scribble homolog isoform X3
XM_009303389.2	XP_009301664.1	protein scribble homolog isoform X7
XM_005166415.3	XP_005166472.1	protein scribble homolog isoform X9
XM_009303393.2	XP_009301668.1	protein scribble homolog isoform X11
XM_021477415.1	XP_021333090.1	protein scribble homolog isoform X2
XM_021477423.1	XP_021333098.1	protein scribble homolog isoform X9
XM_021477428.1	XP_021333103.1	protein scribble homolog isoform X14
XM_021477430.1	XP_021333105.1	protein scribble homolog isoform X16
XM_021477431.1	XP_021333106.1	protein scribble homolog isoform X17

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SCRIB	XP_003586927.1
Mus musculus (house mouse)	Scrib	NP_598850.1
Danio rerio (zebrafish)	scrib	NP_001007176.1
Xenopus tropicalis (tropical clawed frog)	scrib	NP_001243220.1
Pan troglodytes (chimpanzee)	SCRIB	XP_003951409.1
Caenorhabditis elegans (roundworm)	let-413	NP_001023851.1
Homo sapiens (human)	SCRIB	NP_874365.3
Canis lupus familiaris (dog)	SCRIB	XP_005628363.1
Rattus norvegicus (Norway rat)	Scrib	NP_001178808.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Planar cell polarity genes Frizzled3a, Vangl2, and Scribble are required for spinal commissural axon guidance.
Sun SD, Purdy AM, Walsh GS
BMC neuroscience17(1)83(2016 12)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate that anterior guidance decisions by commissural primary ascending (CoPA) axons are dependent on the function of planar cell polarity genes Fzd3a, Vangl2 and Scribble both prior to and after midline crossing; experiments establish CoPA axons as a model system to investigate the mechanism of planar cell polarity signaling in commissural axon guidance.
Title: Planar cell polarity genes Frizzled3a, Vangl2, and Scribble are required for spinal commissural axon guidance.

Suppression of scrib and puf60 in zebrafish leads to reduction in body length, small head size, and craniofacial defects.
Title: SCRIB and PUF60 are primary drivers of the multisystemic phenotypes of the 8q24.3 copy-number variant.

It was found that Scribble is required for oriented cell division and that its function in this process is independent of canonical apicobasal and planar polarity pathways.
Title: Zebrafish neural tube morphogenesis requires Scribble-dependent oriented cell divisions.

Scribble is a candidate link between Fat and the Hippo signaling cascade in vertebrates
Title: Scribble participates in Hippo signaling and is required for normal zebrafish pronephros development.

Lpp interacts with the PCP protein Scrib in zebrafish, and that Lpp and Scrib cooperate for the mediation of convergence and extension.
Title: Lpp is involved in Wnt/PCP signaling and acts together with Scrib to mediate convergence and extension movements during zebrafish gastrulation.

The following scrib gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the scrib cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa143217	XM_021477424.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143218	XM_021477425.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143219	XM_021477426.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143220	XM_021477427.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143221	XM_021477428.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143222	XM_021477429.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143223	XM_021477430.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143224	XM_021477431.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143225	XM_021477432.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa143226	XM_021477433.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa02667	NM_001007175.1 Latest version!	Danio rerio scribble planar cell polarity protein (scrib), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa13339	XM_005166415.3 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40217	XM_009303383.2 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40218	XM_009303384.2 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40219	XM_009303385.2 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40220	XM_009303386.2 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40221	XM_009303387.2 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40222	XM_009303388.2 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40223	XM_009303389.2 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40224	XM_009303390.2 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40225	XM_009303392.2 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40226	XM_009303393.2 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa40227	XM_009303394.2 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa143208	XM_021477414.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa143209	XM_021477415.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa143210	XM_021477416.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa143211	XM_021477417.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa143212	XM_021477418.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa143213	XM_021477419.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa143214	XM_021477421.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa143215	XM_021477422.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa143216	XM_021477423.1 Latest version!	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

scrib ( XM_021477424.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333099.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa143217
Clone ID Related Accession (Same CDS sequence)	XM_021477424.1
Accession Version	XM_021477424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGAT GAAGTCTTTA 
GAGCAGGACG CTTTGAAGGC TCAGATGGTC ATTGCCAAGT CTAAAGAGGG CAAGAAGCGA 
GGAACCCTGG ATCAGCTGTC AGAGTCCCCA TCCCCTGCAC CCACACCCTC TCCAACACCA 
ATGGAAGATA TCAGTCCACG GACAGTGACA TCACCAGGCA GACTGTCCCT GTCAGAAAAG 
AAGTTTGACT ACCGACAATT TGCTGCCATT CCTTCTTCCA AACCCGTTTA CGACATTCAG 
TCTCCAGGTG CAGCTGATGA CGTGCACTTC ATGGATGAGT CCTCCAGTAA CGCAGTGTCT 
GTTATTGATC CAGAAGTTCC AGTGGCCGCC ACGTCAGCTC TAGAAGAGAT GGCTCTCTAC 
AGTAACAAAC GTAAACTACG CCAGGGCCGC CGCAGCCTGG AGGCTCCTGT GCCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333099.1
CDS	1070..7069
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477424.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477424.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGAT GAAGTCTTTA 
GAGCAGGACG CTTTGAAGGC TCAGATGGTC ATTGCCAAGT CTAAAGAGGG CAAGAAGCGA 
GGAACCCTGG ATCAGCTGTC AGAGTCCCCA TCCCCTGCAC CCACACCCTC TCCAACACCA 
ATGGAAGATA TCAGTCCACG GACAGTGACA TCACCAGGCA GACTGTCCCT GTCAGAAAAG 
AAGTTTGACT ACCGACAATT TGCTGCCATT CCTTCTTCCA AACCCGTTTA CGACATTCAG 
TCTCCAGGTG CAGCTGATGA CGTGCACTTC ATGGATGAGT CCTCCAGTAA CGCAGTGTCT 
GTTATTGATC CAGAAGTTCC AGTGGCCGCC ACGTCAGCTC TAGAAGAGAT GGCTCTCTAC 
AGTAACAAAC GTAAACTACG CCAGGGCCGC CGCAGCCTGG AGGCTCCTGT GCCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477425.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333100.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X11, mRNA.

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CloneID	ODa143218
Clone ID Related Accession (Same CDS sequence)	XM_021477425.1
Accession Version	XM_021477425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGACCCCT TCGCCTCTGG GTAGGCACAG TCCAGAACAG 
CGCTCCTTCA AAGACAGACA AAAGTATTTC GAGATCGATG TGAAGCAACA GACTCCAGAC 
AAACCCAAAC CTCGCATCTC ACTGGTGGGA GAAGATGACC TGAAGAAAAT GAAGGAGGAA 
GAAGCAAAGC GAATTGAACA GAGGGCGCGA GAGTTCATGT TGGATGAAGA TGAGGAGGAG 
GAAGAAGAGG ATCTTGCCAA GCAAGTGGCA CATATGAAGG CTCACGGAAA GGTTGTTCTT 
GATGGTGTGG AGTACAAGGT AGAGAGCTTG GGTTCCCCGA CTTCACGGCA GTGTGCCACA 
CCACCAAACT ATAGTGCCAC ACCACCCAGC CACTGCGGAA GCTCAGGTCC TTCCTCTATC 
GACGGTAAAG GAGACTCCCA GCGTAATTCA GTGGAAGACA GTTTCAGACT GGAGCAGAGA 
CCCAATTCTA TGACCGGATT GATCCCAGTT TATCCCGGTG AATCTGCGGC TCCCATCCGG 
ACGGCCAAAG CCGAGCGCAG GCATCAAGAT CGATTACGCA TGCAGAGCCC AGAGCTCACT 
GTCGCTTTGG ACAAGGAGCT GTCGCCTGCT GAAAAACGAG CCCTGGAGGC AGAGAAGAGA 
GCCATGTGGA GAGCAGCCAG GCCTTATGGC TTAGAGGAGG ACGTAAGGCA GTATGAGCAA 
GACCTGGCTA AGCGGCTCTA CCAGTCCCGT GTGAGGGCAT CACTGGGCAG CGCTGCAGCC 
CCTGCCTCTT CCTCCTGCTC CTCTTCCTCT TCCTGTCAGC CCAGGATGAA GTCTTTAGAG 
CAGGACGCTT TGAAGGCTCA GATGGTCATT GCCAAGTCTA AAGAGGGCAA GAAGCGAGGA 
ACCCTGGATC AGCTGTCAGA GTCCCCATCC CCTGCACCCA CACCCTCTCC AACACCAATG 
GAAGATATCA GTCCACGGAC AGTGACATCA CCAGGCAGAC TGTCCCTGTC AGAAAAGAAG 
TTTGACTACC GACAATTTGC TGCCATTCCT TCTTCCAAAC CCGTTTACGA CATTCAGTCT 
CCAGGTGCAG CTGATGACGT GCACTTCATG GATGAGTCCT CCAGTAACGC AGTGTCTGTT 
ATTGATCCAG AAGTTCCAGT GGCCGCCACG TCAGCTCTAG AAGAGATGGC TCTCTACAGT 
AACAAACGTA AACTACGCCA GGGCCGCCGC AGCCTGGAGG CTCCTGTGCC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333100.1
CDS	1070..7066
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPETPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477425.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477425.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGACCCCT TCGCCTCTGG GTAGGCACAG TCCAGAACAG 
CGCTCCTTCA AAGACAGACA AAAGTATTTC GAGATCGATG TGAAGCAACA GACTCCAGAC 
AAACCCAAAC CTCGCATCTC ACTGGTGGGA GAAGATGACC TGAAGAAAAT GAAGGAGGAA 
GAAGCAAAGC GAATTGAACA GAGGGCGCGA GAGTTCATGT TGGATGAAGA TGAGGAGGAG 
GAAGAAGAGG ATCTTGCCAA GCAAGTGGCA CATATGAAGG CTCACGGAAA GGTTGTTCTT 
GATGGTGTGG AGTACAAGGT AGAGAGCTTG GGTTCCCCGA CTTCACGGCA GTGTGCCACA 
CCACCAAACT ATAGTGCCAC ACCACCCAGC CACTGCGGAA GCTCAGGTCC TTCCTCTATC 
GACGGTAAAG GAGACTCCCA GCGTAATTCA GTGGAAGACA GTTTCAGACT GGAGCAGAGA 
CCCAATTCTA TGACCGGATT GATCCCAGTT TATCCCGGTG AATCTGCGGC TCCCATCCGG 
ACGGCCAAAG CCGAGCGCAG GCATCAAGAT CGATTACGCA TGCAGAGCCC AGAGCTCACT 
GTCGCTTTGG ACAAGGAGCT GTCGCCTGCT GAAAAACGAG CCCTGGAGGC AGAGAAGAGA 
GCCATGTGGA GAGCAGCCAG GCCTTATGGC TTAGAGGAGG ACGTAAGGCA GTATGAGCAA 
GACCTGGCTA AGCGGCTCTA CCAGTCCCGT GTGAGGGCAT CACTGGGCAG CGCTGCAGCC 
CCTGCCTCTT CCTCCTGCTC CTCTTCCTCT TCCTGTCAGC CCAGGATGAA GTCTTTAGAG 
CAGGACGCTT TGAAGGCTCA GATGGTCATT GCCAAGTCTA AAGAGGGCAA GAAGCGAGGA 
ACCCTGGATC AGCTGTCAGA GTCCCCATCC CCTGCACCCA CACCCTCTCC AACACCAATG 
GAAGATATCA GTCCACGGAC AGTGACATCA CCAGGCAGAC TGTCCCTGTC AGAAAAGAAG 
TTTGACTACC GACAATTTGC TGCCATTCCT TCTTCCAAAC CCGTTTACGA CATTCAGTCT 
CCAGGTGCAG CTGATGACGT GCACTTCATG GATGAGTCCT CCAGTAACGC AGTGTCTGTT 
ATTGATCCAG AAGTTCCAGT GGCCGCCACG TCAGCTCTAG AAGAGATGGC TCTCTACAGT 
AACAAACGTA AACTACGCCA GGGCCGCCGC AGCCTGGAGG CTCCTGTGCC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477426.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333101.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X12, mRNA.

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CloneID	ODa143219
Clone ID Related Accession (Same CDS sequence)	XM_021477426.1
Accession Version	XM_021477426.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGCC TTATGGCTTA 
GAGGAGGACG TAAGGCAGTA TGAGCAAGAC CTGGCTAAGC GGCTCTACCA GTCCCGTGTG 
AGGGCATCAC TGGGCAGCGC TGCAGCCCCT GCCTCTTCCT CCTGCTCCTC TTCCTCTTCC 
TGTCAGCCCA GGATGAAGTC TTTAGAGCAG GACGCTTTGA AGGCTCAGAT GGTCATTGCC 
AAGTCTAAAG AGGGCAAGAA GCGAGGAACC CTGGATCAGC TGTCAGAGTC CCCATCCCCT 
GCACCCACAC CCTCTCCAAC ACCAATGGAA GATATCAGTC CACGGACAGT GACATCACCA 
GGCAGACTGT CTCCAGGTGC AGCTGATGAC GTGCACTTCA TGGATGAGTC CTCCAGTAAC 
GCAGGCGACT TCCTTGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333101.1
CDS	1071..7031
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSPGAADDVHFMDESSSNAGDFLG

Target ORF information:

RefSeq Version	XM_021477426.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477426.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGCC TTATGGCTTA 
GAGGAGGACG TAAGGCAGTA TGAGCAAGAC CTGGCTAAGC GGCTCTACCA GTCCCGTGTG 
AGGGCATCAC TGGGCAGCGC TGCAGCCCCT GCCTCTTCCT CCTGCTCCTC TTCCTCTTCC 
TGTCAGCCCA GGATGAAGTC TTTAGAGCAG GACGCTTTGA AGGCTCAGAT GGTCATTGCC 
AAGTCTAAAG AGGGCAAGAA GCGAGGAACC CTGGATCAGC TGTCAGAGTC CCCATCCCCT 
GCACCCACAC CCTCTCCAAC ACCAATGGAA GATATCAGTC CACGGACAGT GACATCACCA 
GGCAGACTGT CTCCAGGTGC AGCTGATGAC GTGCACTTCA TGGATGAGTC CTCCAGTAAC 
GCAGGCGACT TCCTTGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477427.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333102.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X13, mRNA.

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CloneID	ODa143220
Clone ID Related Accession (Same CDS sequence)	XM_021477427.1
Accession Version	XM_021477427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGAT GAAGTCTTTA 
GAGCAGGACG CTTTGAAGGC TCAGATGGTC ATTGCCAAGT CTAAAGAGGG CAAGAAGCGA 
GGAACCCTGG ATCAGCTGTC AGAGTCCCCA TCCCCTGCAC CCACACCCTC TCCAACACCA 
ATGGAAGATA TCAGTCCACG GACAGTGACA TCACCAGGCA GACTGTCTCC AGGTGCAGCT 
GATGACGTGC ACTTCATGGA TGAGTCCTCC AGTAACGCAG TGTCTGTTAT TGATCCAGAA 
GTTCCAGTGG CCGCCACGTC AGCTCTAGAA GAGATGGCTC TCTACAGTAA CAAACGTAAA 
CTACGCCAGG GCCGCCGCAG CCTGGAGGCT CCTGTGCCCA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333102.1
CDS	1070..6994
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477427.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477427.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGAT GAAGTCTTTA 
GAGCAGGACG CTTTGAAGGC TCAGATGGTC ATTGCCAAGT CTAAAGAGGG CAAGAAGCGA 
GGAACCCTGG ATCAGCTGTC AGAGTCCCCA TCCCCTGCAC CCACACCCTC TCCAACACCA 
ATGGAAGATA TCAGTCCACG GACAGTGACA TCACCAGGCA GACTGTCTCC AGGTGCAGCT 
GATGACGTGC ACTTCATGGA TGAGTCCTCC AGTAACGCAG TGTCTGTTAT TGATCCAGAA 
GTTCCAGTGG CCGCCACGTC AGCTCTAGAA GAGATGGCTC TCTACAGTAA CAAACGTAAA 
CTACGCCAGG GCCGCCGCAG CCTGGAGGCT CCTGTGCCCA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477428.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333103.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X14, mRNA.

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CloneID	ODa143221
Clone ID Related Accession (Same CDS sequence)	XM_021477428.1
Accession Version	XM_021477428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTGATCTTTA TTCTCCACGG 
AACAATGTCA GTGCAAAACA GCCATCACCT GAGACCCCTT CGCCTCTGGG TAGGCACAGT 
CCAGAACAGC GCTCCTTCAA AGACAGACAA AAGTATTTCG AGATCGATGT GAAGCAACAG 
ACTCCAGACA AACCCAAACC TCGCATCTCA CTGGTGGGAG AAGATGACCT GAAGAAAATG 
AAGGAGGAAG AAGCAAAGCG AATTGAACAG AGGGCGCGAG AGTTCATGTT GGATGAAGAT 
GAGGAGGAGG AAGAAGAGGA TCTTGCCAAG CAAGTGGCAC ATATGAAGGC TCACGGAAAG 
GTTGTTCTTG ATGGTGTGGA GTACAAGGTA GAGAGCTTGG GTTCCCCGAC TTCACGGCAG 
TGTGCCACAC CACCAAACTA TAGTGCCACA CCACCCAGCC ACTGCGGAAG CTCAGGTCCT 
TCCTCTATCG ACGGTAAAGG AGACTCCCAG CGTAATTCAG TGGAAGACAG TTTCAGACTG 
GAGCAGAGAC CCAATTCTAT GACCGGATTG ATCCCAGTTT ATCCCGGTGA ATCTGCGGCT 
CCCATCCGGA CGGCCAAAGC CGAGCGCAGG CATCAAGATC GATTACGCAT GCAGAGCCCA 
GAGCTCACTG TCGCTTTGGA CAAGGAGCTG TCGCCTGCTG AAAAACGAGC CCTGGAGGCA 
GAGAAGAGAG CCATGTGGAG AGCAGCCAGG CCTTATGGCT TAGAGGAGGA CGTAAGGCAG 
TATGAGCAAG ACCTGGCTAA GCGGCTCTAC CAGTCCCGTG TGAGGGCATC ACTGGGCAGC 
GCTGCAGCCC CTGCCTCTTC CTCCTGCTCC TCTTCCTCTT CCTGTCAGCC CAGGATGAAG 
TCTTTAGAGC AGGACGCTTT GAAGGCTCAG ATGGTCATTG CCAAGTCTAA AGAGGGCAAG 
AAGCGAGGAA CCCTGGATCA GCTGTCAGAG TCCCCATCCC CTGCACCCAC ACCCTCTCCA 
ACACCAATGG AAGATATCAG TCCACGGACA GTGACATCAC CAGGCAGACT GTCCCTGTCA 
GAAAAGAAGT TTGACTACCG ACAATTTGCT GCCATTCCTT CTTCCAAACC CGTTTACGAC 
ATTCAGTCTC CAGGTGCAGC TGATGACGTG CACTTCATGG ATGAGTCCTC CAGTAACGCA 
GTGTCTGTTA TTGATCCAGA AGTTCCAGTG GCCGCCACGT CAGCTCTAGA AGAGATGGCT 
CTCTACAGTA ACAAACGTAA ACTACGCCAG GGCCGCCGCA GCCTGGAGGC TCCTGTGCCC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333103.1
CDS	1069..6954
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTDLYSPRNNVSAKQPSPETPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477428.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477428.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTGATCTTTA TTCTCCACGG 
AACAATGTCA GTGCAAAACA GCCATCACCT GAGACCCCTT CGCCTCTGGG TAGGCACAGT 
CCAGAACAGC GCTCCTTCAA AGACAGACAA AAGTATTTCG AGATCGATGT GAAGCAACAG 
ACTCCAGACA AACCCAAACC TCGCATCTCA CTGGTGGGAG AAGATGACCT GAAGAAAATG 
AAGGAGGAAG AAGCAAAGCG AATTGAACAG AGGGCGCGAG AGTTCATGTT GGATGAAGAT 
GAGGAGGAGG AAGAAGAGGA TCTTGCCAAG CAAGTGGCAC ATATGAAGGC TCACGGAAAG 
GTTGTTCTTG ATGGTGTGGA GTACAAGGTA GAGAGCTTGG GTTCCCCGAC TTCACGGCAG 
TGTGCCACAC CACCAAACTA TAGTGCCACA CCACCCAGCC ACTGCGGAAG CTCAGGTCCT 
TCCTCTATCG ACGGTAAAGG AGACTCCCAG CGTAATTCAG TGGAAGACAG TTTCAGACTG 
GAGCAGAGAC CCAATTCTAT GACCGGATTG ATCCCAGTTT ATCCCGGTGA ATCTGCGGCT 
CCCATCCGGA CGGCCAAAGC CGAGCGCAGG CATCAAGATC GATTACGCAT GCAGAGCCCA 
GAGCTCACTG TCGCTTTGGA CAAGGAGCTG TCGCCTGCTG AAAAACGAGC CCTGGAGGCA 
GAGAAGAGAG CCATGTGGAG AGCAGCCAGG CCTTATGGCT TAGAGGAGGA CGTAAGGCAG 
TATGAGCAAG ACCTGGCTAA GCGGCTCTAC CAGTCCCGTG TGAGGGCATC ACTGGGCAGC 
GCTGCAGCCC CTGCCTCTTC CTCCTGCTCC TCTTCCTCTT CCTGTCAGCC CAGGATGAAG 
TCTTTAGAGC AGGACGCTTT GAAGGCTCAG ATGGTCATTG CCAAGTCTAA AGAGGGCAAG 
AAGCGAGGAA CCCTGGATCA GCTGTCAGAG TCCCCATCCC CTGCACCCAC ACCCTCTCCA 
ACACCAATGG AAGATATCAG TCCACGGACA GTGACATCAC CAGGCAGACT GTCCCTGTCA 
GAAAAGAAGT TTGACTACCG ACAATTTGCT GCCATTCCTT CTTCCAAACC CGTTTACGAC 
ATTCAGTCTC CAGGTGCAGC TGATGACGTG CACTTCATGG ATGAGTCCTC CAGTAACGCA 
GTGTCTGTTA TTGATCCAGA AGTTCCAGTG GCCGCCACGT CAGCTCTAGA AGAGATGGCT 
CTCTACAGTA ACAAACGTAA ACTACGCCAG GGCCGCCGCA GCCTGGAGGC TCCTGTGCCC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477429.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333104.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X15, mRNA.

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CloneID	ODa143222
Clone ID Related Accession (Same CDS sequence)	XM_021477429.1
Accession Version	XM_021477429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGCAGT CTCACAAATC TTCCTCTGCG 
ATGCTTGGAA GAGAGGTTCG ATATTCAAAC ATTCATTTCC CTTCAAATGC AAACACCAAG 
GATAATGCAT CAACTAAACC TGGTGCCATC CAGCCTCTCT CTCGGGTGCG ACCCCCTGTG 
TCTCCAGCCA GTCAGGATGG CCACAGTAGT CCAAACCCCT TCCAGCATGG CCTCTCTCCC 
ATCAACTCTC AGACCACTCC TCGTGCCCCA TCCCCTGACA CACATGAGGA ATACCCTATC 
AATATTAAGC AGGTTTATAA GGCCTTCGCT GCTGTGCCAC ACTCCCTGGC CGTACTGGAA 
CCCCCTCAGG ATCTTTATTC TCCACGGAAC AATGTCAGTG CAAAACAGCC ATCACCTGAG 
TTTCAGCCTG AGCTGAATAA TGAAGTGTTT ATGGATGAGT GTGATGGTGA GGGCAGTGGT 
CCTCGTCCCT CCCTCTTATC TGACCGCAGC GAGTACAGAA GCCTGGCAGC TGTGCCACGC 
CTGTCTCGCC CTTCAGTGGA GCCAAAGACC CCTTCGCCTC TGGGTAGGCA CAGTCCAGAA 
CAGCGCTCCT TCAAAGACAG ACAAAAGTAT TTCGAGATCG ATGTGAAGCA ACAGACTCCA 
GACAAACCCA AACCTCGCAT CTCACTGGTG GGAGAAGATG ACCTGAAGAA AATGAAGGAG 
GAAGAAGCAA AGCGAATTGA ACAGAGGGCG CGAGAGTTCA TGTTGGATGA AGATGAGGAG 
GAGGAAGAAG AGGATCTTGC CAAGCAAGTG GCACATATGA AGGCTCACGG AAAGGTTGTT 
CTTGATGGTG TGGAGTACAA GGTAGAGAGC TTGGGTTCCC CGACTTCACG GCAGTGTGCC 
ACACCACCAA ACTATAGTGC CACACCACCC AGCCACTGCG GAAGCTCAGG TCCTTCCTCT 
ATCGACGGTA AAGGAGACTC CCAGCGTAAT TCAGTGGAAG ACAGTTTCAG ACTGGAGCAG 
AGACCCAATT CTATGACCGG ATTGATCCCA GTTTATCCCG GTGAATCTGC GGCTCCCATC 
CGGACGGCCA AAGCCGAGCG CAGGCATCAA GATCGATTAC GCATGCAGAG CCCAGAGCTC 
ACTGTCGCTT TGGACAAGGA GCTGTCGCCT GCTGAAAAAC GAGCCCTGGA GGCAGAGAAG 
AGAGCCATGT GGAGAGCAGC CAGGCCTTAT GGCTTAGAGG AGGACGTAAG GCAGTATGAG 
CAAGACCTGG CTAAGCGGCT CTACCAGTCC CGTGTGAGGG CATCACTGGG CAGCGCTGCA 
GCCCCTGCCT CTTCCTCCTG CTCCTCTTCC TCTTCCTGTC AGCCCAGGAT GAAGTCTTTA 
GAGCAGGACG CTTTGAAGGC TCAGATGGTC ATTGCCAAGT CTAAAGAGGG CAAGAAGCGA 
GGAACCCTGG ATCAGCTGTC AGAGTCCCCA TCCCCTGCAC CCACACCCTC TCCAACACCA 
ATGGAAGATA TCAGTCCACG GACAGTGACA TCACCAGGCA GACTGTCCCT GTCAGAAAAG 
AAGTTTGACT ACCGACAATT TGCTGCCATT CCTTCTTCCA AACCCGTTTA CGACATTCAG 
TCTCCAGGTG CAGCTGATGA CGTGCACTTC ATGGATGAGT CCTCCAGTAA CGCAGTGTCT 
GTTATTGATC CAGAAGTTCC AGTGGCCGCC ACGTCAGCTC TAGAAGAGAT GGCTCTCTAC 
AGTAACAAAC GTAAACTACG CCAGGGCCGC CGCAGCCTGG AGGCTCCTGT GCCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333104.1
CDS	1069..6828
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477429.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477429.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGCAGT CTCACAAATC TTCCTCTGCG 
ATGCTTGGAA GAGAGGTTCG ATATTCAAAC ATTCATTTCC CTTCAAATGC AAACACCAAG 
GATAATGCAT CAACTAAACC TGGTGCCATC CAGCCTCTCT CTCGGGTGCG ACCCCCTGTG 
TCTCCAGCCA GTCAGGATGG CCACAGTAGT CCAAACCCCT TCCAGCATGG CCTCTCTCCC 
ATCAACTCTC AGACCACTCC TCGTGCCCCA TCCCCTGACA CACATGAGGA ATACCCTATC 
AATATTAAGC AGGTTTATAA GGCCTTCGCT GCTGTGCCAC ACTCCCTGGC CGTACTGGAA 
CCCCCTCAGG ATCTTTATTC TCCACGGAAC AATGTCAGTG CAAAACAGCC ATCACCTGAG 
TTTCAGCCTG AGCTGAATAA TGAAGTGTTT ATGGATGAGT GTGATGGTGA GGGCAGTGGT 
CCTCGTCCCT CCCTCTTATC TGACCGCAGC GAGTACAGAA GCCTGGCAGC TGTGCCACGC 
CTGTCTCGCC CTTCAGTGGA GCCAAAGACC CCTTCGCCTC TGGGTAGGCA CAGTCCAGAA 
CAGCGCTCCT TCAAAGACAG ACAAAAGTAT TTCGAGATCG ATGTGAAGCA ACAGACTCCA 
GACAAACCCA AACCTCGCAT CTCACTGGTG GGAGAAGATG ACCTGAAGAA AATGAAGGAG 
GAAGAAGCAA AGCGAATTGA ACAGAGGGCG CGAGAGTTCA TGTTGGATGA AGATGAGGAG 
GAGGAAGAAG AGGATCTTGC CAAGCAAGTG GCACATATGA AGGCTCACGG AAAGGTTGTT 
CTTGATGGTG TGGAGTACAA GGTAGAGAGC TTGGGTTCCC CGACTTCACG GCAGTGTGCC 
ACACCACCAA ACTATAGTGC CACACCACCC AGCCACTGCG GAAGCTCAGG TCCTTCCTCT 
ATCGACGGTA AAGGAGACTC CCAGCGTAAT TCAGTGGAAG ACAGTTTCAG ACTGGAGCAG 
AGACCCAATT CTATGACCGG ATTGATCCCA GTTTATCCCG GTGAATCTGC GGCTCCCATC 
CGGACGGCCA AAGCCGAGCG CAGGCATCAA GATCGATTAC GCATGCAGAG CCCAGAGCTC 
ACTGTCGCTT TGGACAAGGA GCTGTCGCCT GCTGAAAAAC GAGCCCTGGA GGCAGAGAAG 
AGAGCCATGT GGAGAGCAGC CAGGCCTTAT GGCTTAGAGG AGGACGTAAG GCAGTATGAG 
CAAGACCTGG CTAAGCGGCT CTACCAGTCC CGTGTGAGGG CATCACTGGG CAGCGCTGCA 
GCCCCTGCCT CTTCCTCCTG CTCCTCTTCC TCTTCCTGTC AGCCCAGGAT GAAGTCTTTA 
GAGCAGGACG CTTTGAAGGC TCAGATGGTC ATTGCCAAGT CTAAAGAGGG CAAGAAGCGA 
GGAACCCTGG ATCAGCTGTC AGAGTCCCCA TCCCCTGCAC CCACACCCTC TCCAACACCA 
ATGGAAGATA TCAGTCCACG GACAGTGACA TCACCAGGCA GACTGTCCCT GTCAGAAAAG 
AAGTTTGACT ACCGACAATT TGCTGCCATT CCTTCTTCCA AACCCGTTTA CGACATTCAG 
TCTCCAGGTG CAGCTGATGA CGTGCACTTC ATGGATGAGT CCTCCAGTAA CGCAGTGTCT 
GTTATTGATC CAGAAGTTCC AGTGGCCGCC ACGTCAGCTC TAGAAGAGAT GGCTCTCTAC 
AGTAACAAAC GTAAACTACG CCAGGGCCGC CGCAGCCTGG AGGCTCCTGT GCCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477430.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333105.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X16, mRNA.

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CloneID	ODa143223
Clone ID Related Accession (Same CDS sequence)	XM_021477430.1
Accession Version	XM_021477430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGTTTCAGCC TGAGCTGAAT AATGAAGTGT TTATGGATGA GTGTGATGGT 
GAGGGCAGTG GTCCTCGTCC CTCCCTCTTA TCTGACCGCA GCGAGTACAG AAGCCTGGCA 
GCTGTGCCAC GCCTGTCTCG CCCTTCAGTG GAGCCAAAGA CCCCTTCGCC TCTGGGTAGG 
CACAGTCCAG AACAGCGCTC CTTCAAAGAC AGACAAAAGT ATTTCGAGAT CGATGTGAAG 
CAACAGACTC CAGACAAACC CAAACCTCGC ATCTCACTGG TGGGAGAAGA TGACCTGAAG 
AAAATGAAGG AGGAAGAAGC AAAGCGAATT GAACAGAGGG CGCGAGAGTT CATGTTGGAT 
GAAGATGAGG AGGAGGAAGA AGAGGATCTT GCCAAGCAAG TGGCACATAT GAAGGCTCAC 
GGAAAGGTTG TTCTTGATGG TGTGGAGTAC AAGGTAGAGA GCTTGGGTTC CCCGACTTCA 
CGGCAGTGTG CCACACCACC AAACTATAGT GCCACACCAC CCAGCCACTG CGGAAGCTCA 
GGTCCTTCCT CTATCGACGG TAAAGGAGAC TCCCAGCGTA ATTCAGTGGA AGACAGTTTC 
AGACTGGAGC AGAGACCCAA TTCTATGACC GGATTGATCC CAGTTTATCC CGGTGAATCT 
GCGGCTCCCA TCCGGACGGC CAAAGCCGAG CGCAGGCATC AAGATCGATT ACGCATGCAG 
AGCCCAGAGC TCACTGTCGC TTTGGACAAG GAGCTGTCGC CTGCTGAAAA ACGAGCCCTG 
GAGGCAGAGA AGAGAGCCAT GTGGAGAGCA GCCAGGCCTT ATGGCTTAGA GGAGGACGTA 
AGGCAGTATG AGCAAGACCT GGCTAAGCGG CTCTACCAGT CCCGTGTGAG GGCATCACTG 
GGCAGCGCTG CAGCCCCTGC CTCTTCCTCC TGCTCCTCTT CCTCTTCCTG TCAGCCCAGG 
ATGAAGTCTT TAGAGCAGGA CGCTTTGAAG GCTCAGATGG TCATTGCCAA GTCTAAAGAG 
GGCAAGAAGC GAGGAACCCT GGATCAGCTG TCAGAGTCCC CATCCCCTGC ACCCACACCC 
TCTCCAACAC CAATGGAAGA TATCAGTCCA CGGACAGTGA CATCACCAGG CAGACTGTCC 
CTGTCAGAAA AGAAGTTTGA CTACCGACAA TTTGCTGCCA TTCCTTCTTC CAAACCCGTT 
TACGACATTC AGTCTCCAGG TGCAGCTGAT GACGTGCACT TCATGGATGA GTCCTCCAGT 
AACGCAGTGT CTGTTATTGA TCCAGAAGTT CCAGTGGCCG CCACGTCAGC TCTAGAAGAG 
ATGGCTCTCT ACAGTAACAA ACGTAAACTA CGCCAGGGCC GCCGCAGCCT GGAGGCTCCT 
GTGCCCACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333105.1
CDS	1068..6779
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477430.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477430.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGTTTCAGCC TGAGCTGAAT AATGAAGTGT TTATGGATGA GTGTGATGGT 
GAGGGCAGTG GTCCTCGTCC CTCCCTCTTA TCTGACCGCA GCGAGTACAG AAGCCTGGCA 
GCTGTGCCAC GCCTGTCTCG CCCTTCAGTG GAGCCAAAGA CCCCTTCGCC TCTGGGTAGG 
CACAGTCCAG AACAGCGCTC CTTCAAAGAC AGACAAAAGT ATTTCGAGAT CGATGTGAAG 
CAACAGACTC CAGACAAACC CAAACCTCGC ATCTCACTGG TGGGAGAAGA TGACCTGAAG 
AAAATGAAGG AGGAAGAAGC AAAGCGAATT GAACAGAGGG CGCGAGAGTT CATGTTGGAT 
GAAGATGAGG AGGAGGAAGA AGAGGATCTT GCCAAGCAAG TGGCACATAT GAAGGCTCAC 
GGAAAGGTTG TTCTTGATGG TGTGGAGTAC AAGGTAGAGA GCTTGGGTTC CCCGACTTCA 
CGGCAGTGTG CCACACCACC AAACTATAGT GCCACACCAC CCAGCCACTG CGGAAGCTCA 
GGTCCTTCCT CTATCGACGG TAAAGGAGAC TCCCAGCGTA ATTCAGTGGA AGACAGTTTC 
AGACTGGAGC AGAGACCCAA TTCTATGACC GGATTGATCC CAGTTTATCC CGGTGAATCT 
GCGGCTCCCA TCCGGACGGC CAAAGCCGAG CGCAGGCATC AAGATCGATT ACGCATGCAG 
AGCCCAGAGC TCACTGTCGC TTTGGACAAG GAGCTGTCGC CTGCTGAAAA ACGAGCCCTG 
GAGGCAGAGA AGAGAGCCAT GTGGAGAGCA GCCAGGCCTT ATGGCTTAGA GGAGGACGTA 
AGGCAGTATG AGCAAGACCT GGCTAAGCGG CTCTACCAGT CCCGTGTGAG GGCATCACTG 
GGCAGCGCTG CAGCCCCTGC CTCTTCCTCC TGCTCCTCTT CCTCTTCCTG TCAGCCCAGG 
ATGAAGTCTT TAGAGCAGGA CGCTTTGAAG GCTCAGATGG TCATTGCCAA GTCTAAAGAG 
GGCAAGAAGC GAGGAACCCT GGATCAGCTG TCAGAGTCCC CATCCCCTGC ACCCACACCC 
TCTCCAACAC CAATGGAAGA TATCAGTCCA CGGACAGTGA CATCACCAGG CAGACTGTCC 
CTGTCAGAAA AGAAGTTTGA CTACCGACAA TTTGCTGCCA TTCCTTCTTC CAAACCCGTT 
TACGACATTC AGTCTCCAGG TGCAGCTGAT GACGTGCACT TCATGGATGA GTCCTCCAGT 
AACGCAGTGT CTGTTATTGA TCCAGAAGTT CCAGTGGCCG CCACGTCAGC TCTAGAAGAG 
ATGGCTCTCT ACAGTAACAA ACGTAAACTA CGCCAGGGCC GCCGCAGCCT GGAGGCTCCT 
GTGCCCACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477431.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333106.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X17, mRNA.

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CloneID	ODa143224
Clone ID Related Accession (Same CDS sequence)	XM_021477431.1
Accession Version	XM_021477431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGAGTT TGAGCTCTGG ACCACTGAAA CTGGACTATA AGACACTGGC GGCTCTGCCT 
ACAACTAGTC TACAGAAAGT TAATAGAGCT TCACCATCTG AGCCTTTCAG GATTGACAGT 
CCTGTCCGAG ATGCTGCACA CTCTCCACAC AACTCACAGC AGTCTCACAA ATCTTCCTCT 
GCGATGCTTG GAAGAGAGGT TCGATATTCA AACATTCATT TCCCTTCAAA TGCAAACACC 
AAGGATAATG CATCAACTAA ACCTGGTGCC ATCCAGCCTC TCTCTCGGGT GCGACCCCCT 
GTGTCTCCAG CCAGTCAGGA TGGCCACAGT AGTCCAAACC CCTTCCAGCA TGGCCTCTCT 
CCCATCAACT CTCAGACCAC TCCTCGTGCC CCATCCCCTG ACACACATGA GGAATACCCT 
ATCAATATTA AGCAGGTTTA TAAGGCCTTC GCTGCTGTGC CACACTCCCT GGCCGTACTG 
GAACCCCCTC AGGATCTTTA TTCTCCACGG AACAATGTCA GTGCAAAACA GCCATCACCT 
GAGTTTCAGC CTGAGCTGAA TAATGAAGTG TTTATGGATG AGTGTGATGG TGAGGGCAGT 
GGTCCTCGTC CCTCCCTCTT ATCTGACCGC AGCGAGTACA GAAGCCTGGC AGCTGTGCCA 
CGCCTGTCTC GCCCTTCAGT GGAGCCAAAG ACCCCTTCGC CTCTGGGTAG GCACAGTCCA 
GAACAGCGCT CCTTCAAAGA CAGACAAAAG TATTTCGAGA TCGATGTGAA GCAACAGACT 
CCAGACAAAC CCAAACCTCG CATCTCACTG GTGGGAGAAG ATGACCTGAA GAAAATGAAG 
GAGGAAGAAG CAAAGCGAAT TGAACAGAGG GCGCGAGAGT TCATGTTGGA TGAAGATGAG 
GAGGAGGAAG AAGAGGATCT TGCCAAGCAA GTGGCACATA TGAAGGCTCA CGGAAAGGTT 
GTTCTTGATG GTGTGGAGTA CAAGGTAGAG AGCTTGGGTT CCCCGACTTC ACGGCAGTGT 
GCCACACCAC CAAACTATAG TGCCACACCA CCCAGCCACT GCGGAAGCTC AGGTCCTTCC 
TCTATCGACG GTAAAGGAGA CTCCCAGCGT AATTCAGTGG AAGACAGTTT CAGACTGGAG 
CAGAGACCCA ATTCTATGAC CGGATTGATC CCAGTTTATC CCGGTGAATC TGCGGCTCCC 
ATCCGGACGG CCAAAGCCGA GCGCAGGCAT CAAGATCGAT TACGCATGCA GAGCCCAGAG 
CTCACTGTCG CTTTGGACAA GGAGCTGTCG CCTGCTGAAA AACGAGCCCT GGAGGCAGAG 
AAGAGAGCCA TGTGGAGAGC AGCCAGGCCT TATGGCTTAG AGGAGGACGT AAGGCAGTAT 
GAGCAAGACC TGGCTAAGCG GCTCTACCAG TCCCGTGTGA GGGCATCACT GGGCAGCGCT 
GCAGCCCCTG CCTCTTCCTC CTGCTCCTCT TCCTCTTCCT GTCAGCCCAG GATGAAGTCT 
TTAGAGCAGG ACGCTTTGAA GGCTCAGATG GTCATTGCCA AGTCTAAAGA GGGCAAGAAG 
CGAGGAACCC TGGATCAGCT GTCAGAGTCC CCATCCCCTG CACCCACACC CTCTCCAACA 
CCAATGGAAG ATATCAGTCC ACGGACAGTG ACATCACCAG GCAGACTGTC CCTGTCAGAA 
AAGAAGTTTG ACTACCGACA ATTTGCTGCC ATTCCTTCTT CCAAACCCGT TTACGACATT 
CAGTCTCCAG GTGCAGCTGA TGACGTGCAC TTCATGGATG AGTCCTCCAG TAACGCAGTG 
TCTGTTATTG ATCCAGAAGT TCCAGTGGCC GCCACGTCAG CTCTAGAAGA GATGGCTCTC 
TACAGTAACA AACGTAAACT ACGCCAGGGC CGCCGCAGCC TGGAGGCTCC TGTGCCCACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333106.1
CDS	1069..6771
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVSLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477431.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477431.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGAGTT TGAGCTCTGG ACCACTGAAA CTGGACTATA AGACACTGGC GGCTCTGCCT 
ACAACTAGTC TACAGAAAGT TAATAGAGCT TCACCATCTG AGCCTTTCAG GATTGACAGT 
CCTGTCCGAG ATGCTGCACA CTCTCCACAC AACTCACAGC AGTCTCACAA ATCTTCCTCT 
GCGATGCTTG GAAGAGAGGT TCGATATTCA AACATTCATT TCCCTTCAAA TGCAAACACC 
AAGGATAATG CATCAACTAA ACCTGGTGCC ATCCAGCCTC TCTCTCGGGT GCGACCCCCT 
GTGTCTCCAG CCAGTCAGGA TGGCCACAGT AGTCCAAACC CCTTCCAGCA TGGCCTCTCT 
CCCATCAACT CTCAGACCAC TCCTCGTGCC CCATCCCCTG ACACACATGA GGAATACCCT 
ATCAATATTA AGCAGGTTTA TAAGGCCTTC GCTGCTGTGC CACACTCCCT GGCCGTACTG 
GAACCCCCTC AGGATCTTTA TTCTCCACGG AACAATGTCA GTGCAAAACA GCCATCACCT 
GAGTTTCAGC CTGAGCTGAA TAATGAAGTG TTTATGGATG AGTGTGATGG TGAGGGCAGT 
GGTCCTCGTC CCTCCCTCTT ATCTGACCGC AGCGAGTACA GAAGCCTGGC AGCTGTGCCA 
CGCCTGTCTC GCCCTTCAGT GGAGCCAAAG ACCCCTTCGC CTCTGGGTAG GCACAGTCCA 
GAACAGCGCT CCTTCAAAGA CAGACAAAAG TATTTCGAGA TCGATGTGAA GCAACAGACT 
CCAGACAAAC CCAAACCTCG CATCTCACTG GTGGGAGAAG ATGACCTGAA GAAAATGAAG 
GAGGAAGAAG CAAAGCGAAT TGAACAGAGG GCGCGAGAGT TCATGTTGGA TGAAGATGAG 
GAGGAGGAAG AAGAGGATCT TGCCAAGCAA GTGGCACATA TGAAGGCTCA CGGAAAGGTT 
GTTCTTGATG GTGTGGAGTA CAAGGTAGAG AGCTTGGGTT CCCCGACTTC ACGGCAGTGT 
GCCACACCAC CAAACTATAG TGCCACACCA CCCAGCCACT GCGGAAGCTC AGGTCCTTCC 
TCTATCGACG GTAAAGGAGA CTCCCAGCGT AATTCAGTGG AAGACAGTTT CAGACTGGAG 
CAGAGACCCA ATTCTATGAC CGGATTGATC CCAGTTTATC CCGGTGAATC TGCGGCTCCC 
ATCCGGACGG CCAAAGCCGA GCGCAGGCAT CAAGATCGAT TACGCATGCA GAGCCCAGAG 
CTCACTGTCG CTTTGGACAA GGAGCTGTCG CCTGCTGAAA AACGAGCCCT GGAGGCAGAG 
AAGAGAGCCA TGTGGAGAGC AGCCAGGCCT TATGGCTTAG AGGAGGACGT AAGGCAGTAT 
GAGCAAGACC TGGCTAAGCG GCTCTACCAG TCCCGTGTGA GGGCATCACT GGGCAGCGCT 
GCAGCCCCTG CCTCTTCCTC CTGCTCCTCT TCCTCTTCCT GTCAGCCCAG GATGAAGTCT 
TTAGAGCAGG ACGCTTTGAA GGCTCAGATG GTCATTGCCA AGTCTAAAGA GGGCAAGAAG 
CGAGGAACCC TGGATCAGCT GTCAGAGTCC CCATCCCCTG CACCCACACC CTCTCCAACA 
CCAATGGAAG ATATCAGTCC ACGGACAGTG ACATCACCAG GCAGACTGTC CCTGTCAGAA 
AAGAAGTTTG ACTACCGACA ATTTGCTGCC ATTCCTTCTT CCAAACCCGT TTACGACATT 
CAGTCTCCAG GTGCAGCTGA TGACGTGCAC TTCATGGATG AGTCCTCCAG TAACGCAGTG 
TCTGTTATTG ATCCAGAAGT TCCAGTGGCC GCCACGTCAG CTCTAGAAGA GATGGCTCTC 
TACAGTAACA AACGTAAACT ACGCCAGGGC CGCCGCAGCC TGGAGGCTCC TGTGCCCACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477432.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333107.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X18, mRNA.

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CloneID	ODa143225
Clone ID Related Accession (Same CDS sequence)	XM_021477432.1
Accession Version	XM_021477432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGCC TTATGGCTTA 
GAGGAGGACG TAAGGCAGTA TGAGCAAGAC CTGGCTAAGC GGCTCTACCA GTCCCGTGTG 
AGGGCATCAC TGGGCAGCGC TGCAGCCCCT GCCTCTTCCT CCTGCTCCTC TTCCTCTTCC 
TGTCAGCCCA GGATGAAGTC TTTAGAGCAG GACGCTTTGA AGGCTCAGAT GGTCATTGCC 
AAGTCTAAAG AGGGCAAGAA GCGAGGAACC CTGGATCAGC TGTCAGAGTC CCCATCCCCT 
GCACCCACAC CCTCTCCAAC ACCAATGGAA GATATCAGTC CACGGACAGT GACATCACCA 
GGCAGACTGT CCCTGTCAGA AAAGAAGTTT GACTACCGAC AATTTGCTGC CATTCCTTCT 
TCCAAACCCG TTTACGACAT TCAGTCTCCA GGTGCAGCTG ATGACGTGCA CTTCATGGAT 
GAGTCCTCCA GTAACGCAGT GTCTGTTATT GATCCAGAAG TTCCAGTGGC CGCCACGTCA 
GCTCTAGAAG AGATGGCTCT CTACAGTAAC AAACGTAAAC TACGCCAGGG CCGCCGCAGC 
CTGGAGGCTC CTGTGCCCAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333107.1
CDS	1069..6732
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477432.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477432.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGCC TTATGGCTTA 
GAGGAGGACG TAAGGCAGTA TGAGCAAGAC CTGGCTAAGC GGCTCTACCA GTCCCGTGTG 
AGGGCATCAC TGGGCAGCGC TGCAGCCCCT GCCTCTTCCT CCTGCTCCTC TTCCTCTTCC 
TGTCAGCCCA GGATGAAGTC TTTAGAGCAG GACGCTTTGA AGGCTCAGAT GGTCATTGCC 
AAGTCTAAAG AGGGCAAGAA GCGAGGAACC CTGGATCAGC TGTCAGAGTC CCCATCCCCT 
GCACCCACAC CCTCTCCAAC ACCAATGGAA GATATCAGTC CACGGACAGT GACATCACCA 
GGCAGACTGT CCCTGTCAGA AAAGAAGTTT GACTACCGAC AATTTGCTGC CATTCCTTCT 
TCCAAACCCG TTTACGACAT TCAGTCTCCA GGTGCAGCTG ATGACGTGCA CTTCATGGAT 
GAGTCCTCCA GTAACGCAGT GTCTGTTATT GATCCAGAAG TTCCAGTGGC CGCCACGTCA 
GCTCTAGAAG AGATGGCTCT CTACAGTAAC AAACGTAAAC TACGCCAGGG CCGCCGCAGC 
CTGGAGGCTC CTGTGCCCAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477433.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333108.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X19, mRNA.

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CloneID	ODa143226
Clone ID Related Accession (Same CDS sequence)	XM_021477433.1
Accession Version	XM_021477433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGGGTCTT CAGAGGAGAG CCACACCACA CCCTAGTGAG 
TTAAAGGTGA TGAAGAATGT AATTGAAGCA AGAAGAAATG AGGCTTATAC AGCCAGACCA 
GATGAAGATC TAGAATCCCC AGATGCTGAG GAGAAACGGT TAAGTGGTCT GTCCAATCAG 
AGTCATGATT CTCAGGCATC CAGTAGCACC ACCTCAGCCA CTTCCCATGA GGACAAGCAG 
GGTATAAACA GTACAGGGGT GGAGCTTAAT GATGGACAGG TTCAAGAAGA GGAGGATCTG 
GATGAGATGG AGGTGGAGTA TACTGAGCCT ACTGTGCACT TTGCTGAAGA GCCCATCATC 
CGTGGTGGTG ATGAGGACGA CGATTATGAT AATGACGATG ATGACGCTGA AAGGAGTGAC 
GAGGTGTCTC AAACGCCATC TTTTCCTGCC GAGAAACAGC GGCTCATACG TAAAGACACA 
CCACACTACA AAAAGCACTT CAAGATCACC AAGCTGCCCA AACCAGAGAC AGTCGCCGCC 
CTGCTGCAAG GCTTCAGTCA TGATGGCCTG TCACCTACTG CTCACACCCC TGAGAACGAG 
AGGGATGGAG AGGATGACGA GGAAGAGGAG GAGGATGAAG ACGAGGAGGA CGATCTCCAT 
ACTCCTTTCC AGCACAGACT AGATGCAGCA GAGCTAGAAG ACATCAGAAT GAGTCAGATG 
AACTCGTCCC TGCAGCCAGT GAAGGGGGTG TCATTTGATC AAGTCAATAA TCTGCTGATT 
GAACCTGCTC GAATTGAGGA GGAAGAGCTG ACCTTGAGCA TCCTGAGGCA GACTGGTGGA 
TTAGGCATCA GCATAGCAGG AGGGAAGGGA TCTACTCCCT ATAAAGGTGA CGATGAGGGT 
ATTTTCATCT CTAGGGTGTC AGAAGAAGGC CCTGCGGCTC GTGCTGGTGT CAAAGTTGGA 
GACAAACTTC TTGAGGTGAA TGGAGTAGAC CTGCACGGAG CGGAGCATCA TACAGCTGTT 
GAAGCTTTGC GCAATTCAGG AGCAGCCGTG GTCATGACTG TTTTGCGGGA GCGCATGGTG 
GAACCAGAAA ACGCCATCAC CACCACACCG TTGCGGCCAG AAGATGATTA TTTCCCCCGG 
GAGAGACGCT CCAGTGGGCT TCCTTTCCTC CTGGATCCTG ATTGTCCCGC TGTTAGCACT 
GGACCCGCAC AGCGACTAGC CACTTGTCTG ATCCGCAATG ATAAAGGCTT AGGCTTCAGC 
ATTGCTGGTG GGAAAGGGTC AACGCTGTAC AGAGTCGGAG ACACGGGCAT CTTCATCTCT 
CGTATTGCTG AAGGAGGAGC AGCTCACAGA GACAACATCC TGCAAGTGGG AGACAGGGTC 
ATATCAATCA ATGGTGTAGA CATGACAGAG GCCAGGCATG ACCAGGCAGT AGCGCTGCTT 
ACCGGCACCT CCCCCACCAT CACCCTGGTG GTGGATCGGG AACAAAGCAG TGTAGGCGGA 
GCCTCCCCTC GAACCCGACC ACATTCGCCC CCACCTCCAG AGCCCTCTGA CTCCCCTGAG 
CAAGAGGACG GTGGAGACGA GCACCTTGGA AACCATCTCA ACTGCCCCAT GGAAGATGAG 
TACCCTATTG AGGAAGTGAC GCTGATCAAA GCAGGTGGAC CCCTGGGTTT GAGCATTGTA 
GGGGGCAGCG ACCATGCCAG CCATCCATTT GGAATTAATG AACCTGGAGT GTTCATCTCA 
AAGGTCATCC CCAATGGCCT GGCCTCCCAG AGTGGACTTC GCGTGGGTGA TCGTATCTTG 
GAGGTGAACA GCATTGATCT GCGTCATGCC ACACACCAGG AGGCTGTTAG AGCCTTGCTT 
TCCAACAAAC AGGAGATCCG TATGCTTGTG CGCAGAGATC CTTCTCCCCC TGGCATGCAG 
GAAATTGTCA TTCACAAGCA ACCTGGAGAA AAGCTGGGAA TAAGCATTAG GGGCGGAGCT 
AAAGGTCATG CAGGAAACCC CTTTGACCCC ACAGATGAGG GCATCTTTAT TTCCAAGGTG 
AGCTCCAACG GTGCTGCTGC ACGAGATGGC CGACTGCGGG TGGGTATGCG TATTCTTGAG 
GTGGGCAACA ACAGTCTGCT GGGTATGACA CATACAGAAG CAGTGAGGGT CTTGCGTGCT 
TCTGGAGATT CCCTGGTCAT GCTTATTTGT GATGGCTTTG ACCCAAAAAG TGCTTCAACC 
ATAGAGGCAT CTCCTGGTGT TATTGCGAAT CCGTTTGCGG CAGGTATTGT TCGTAAAAAC 
AGTATGGAAA GCATCTCATC AGTCGACAGA GACCTGAGTC CAGAAGAGAT TGACATCATG 
CAGAAGGAGG TAGAAATGGT AAGAGAGACG TCCCAGTGGG AACGAGAGGA GATGGAAAAA 
GTGGAGCGCA TGCGCTTGGA GCGCGAGGAG GCAACTCGCT TGCTGGAGGA GGAAACTGAG 
AGTTTGAGCT CTGGACCACT GAAACTGGAC TATAAGACAC TGGCGGCTCT GCCTACAACT 
AGTCTACAGA AAGTTAATAG ATTTTCTCCC TCCTTTTCCA CATTTGCCAA AATGGAGGCT 
GCCATGCCGG CTGGCCCCGT AGACCCCCTG GGTTTTGAGA AAGTCCATGC TTCTATGTAC 
CATGTAGAAC CTGTTGCCCT CCCTTCATCT CCAATCTGCC CAGCCGCAAG TTCAGCGGCT 
TCAAAACAGC CCCAAGCTGA CTCACGGGAC CTGGTCTTAG ATCTAGGAAT GGCTCAAGAG 
GAGGAGGAAT GTCTTGTGGA CTCCCAACCA ATCTGCTTTA GTGAAAACCC ATTTCTTGTG 
GCTAACCGCA AGGGCAAAGG CTGCCCACCA GAAGAGCGCA TTTTATCAGG GCCACCTGTG 
GGTTACGGCA AGCATGGGCA GCTGCAGCCC TGGTTGTACA GCAAGGCTTC ACCATCTGAG 
CCTTTCAGGA TTGACAGTCC TGTCCGAGAT GCTGCACACT CTCCACACAA CTCACAGCAG 
TCTCACAAAT CTTCCTCTGC GATGCTTGGA AGAGAGGTTC GATATTCAAA CATTCATTTC 
CCTTCAAATG CAAACACCAA GGATAATGCA TCAACTAAAC CTGGTGCCAT CCAGCCTCTC 
TCTCGGGTGC GACCCCCTGT GTCTCCAGCC AGTCAGGATG GCCACAGTAG TCCAAACCCC 
TTCCAGCATG GCCTCTCTCC CATCAACTCT CAGACCACTC CTCGTGCCCC ATCCCCTGAC 
ACACATGAGG AATACCCTAT CAATATTAAG CAGGTTTATA AGGCCTTCGC TGCTGTGCCA 
CACTCCCTGG CCGTACTGGA ACCCCCTCAG GATCTTTATT CTCCACGGAA CAATGTCAGT 
GCAAAACAGC CATCACCTGA GTTTCAGCCT GAGCTGAATA ATGAAGTGTT TATGGATGAG 
TGTGATGGTG AGGGCAGTGG TCCTCGTCCC TCCCTCTTAT CTGACCGCAG CGAGTACAGA 
AGCCTGGCAG CTGTGCCACG CCTGTCTCGC CCTTCAGTGG AGCCAAAGAC CCCTTCGCCT 
CTGGGTAGGC ACAGTCCAGA ACAGCGCTCC TTCAAAGACA GACAAAAGTA TTTCGAGATC 
GATGTGAAGC AACAGACTCC AGACAAACCC AAACCTCGCA TCTCACTGGT GGGAGAAGAT 
GACCTGAAGA AAATGAAGGA GGAAGAAGCA AAGCGAATTG AACAGAGGGC GCGAGAGTTC 
ATGTTGGATG AAGATGAGGA GGAGGAAGAA GAGGATCTTG CCAAGCAAGT GGCACATATG 
AAGGCTCACG GAAAGGTTGT TCTTGATGGT GTGGAGTACA AGGTAGAGAG CTTGGGTTCC 
CCGACTTCAC GGCAGTGTGC CACACCACCA AACTATAGTG CCACACCACC CAGCCACTGC 
GGAAGCTCAG GTCCTTCCTC TATCGACGGT AAAGGAGACT CCCAGCGTAA TTCAGTGGAA 
GACAGTTTCA GACTGGAGCA GAGACCCAAT TCTATGACCG GATTGATCCC AGTTTATCCC 
GGTGAATCTG CGGCTCCCAT CCGGACGGCC AAAGCCGAGC GCAGGCATCA AGATCGATTA 
CGCATGCAGA GCCCAGAGCT CACTGTCGCT TTGGACAAGG AGCTGTCGCC TGCTGAAAAA 
CGAGCCCTGG AGGCAGAGAA GAGAGCCATG TGGAGAGCAG CCAGGATGAA GTCTTTAGAG 
CAGGACGCTT TGAAGGCTCA GATGGTCATT GCCAAGTCTA AAGAGGGCAA GAAGCGAGGA 
ACCCTGGATC AGCTGTCAGA GTCCCCATCC CCTGCACCCA CACCCTCTCC AACACCAATG 
GAAGATATCA GTCCACGGAC AGTGACATCA CCAGGCAGAC TGTCCCTGTC AGAAAAGAAG 
TTTGACTACC GACAATTTGC TGCCATTCCT TCTTCCAAAC CCGTTTACGA CATTCAGTCT 
CCAGGTGCAG CTGATGACGT GCACTTCATG GATGAGTCCT CCAGTAACGC AGTGTCTGTT 
ATTGATCCAG AAGTTCCAGT GGCCGCCACG TCAGCTCTAG AAGAGATGGC TCTCTACAGT 
AACAAACGTA AACTACGCCA GGGCCGCCGC AGCCTGGAGG CTCCTGTGCC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333108.1
CDS	1069..7065
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477433.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477433.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGGGTCTT CAGAGGAGAG CCACACCACA CCCTAGTGAG 
TTAAAGGTGA TGAAGAATGT AATTGAAGCA AGAAGAAATG AGGCTTATAC AGCCAGACCA 
GATGAAGATC TAGAATCCCC AGATGCTGAG GAGAAACGGT TAAGTGGTCT GTCCAATCAG 
AGTCATGATT CTCAGGCATC CAGTAGCACC ACCTCAGCCA CTTCCCATGA GGACAAGCAG 
GGTATAAACA GTACAGGGGT GGAGCTTAAT GATGGACAGG TTCAAGAAGA GGAGGATCTG 
GATGAGATGG AGGTGGAGTA TACTGAGCCT ACTGTGCACT TTGCTGAAGA GCCCATCATC 
CGTGGTGGTG ATGAGGACGA CGATTATGAT AATGACGATG ATGACGCTGA AAGGAGTGAC 
GAGGTGTCTC AAACGCCATC TTTTCCTGCC GAGAAACAGC GGCTCATACG TAAAGACACA 
CCACACTACA AAAAGCACTT CAAGATCACC AAGCTGCCCA AACCAGAGAC AGTCGCCGCC 
CTGCTGCAAG GCTTCAGTCA TGATGGCCTG TCACCTACTG CTCACACCCC TGAGAACGAG 
AGGGATGGAG AGGATGACGA GGAAGAGGAG GAGGATGAAG ACGAGGAGGA CGATCTCCAT 
ACTCCTTTCC AGCACAGACT AGATGCAGCA GAGCTAGAAG ACATCAGAAT GAGTCAGATG 
AACTCGTCCC TGCAGCCAGT GAAGGGGGTG TCATTTGATC AAGTCAATAA TCTGCTGATT 
GAACCTGCTC GAATTGAGGA GGAAGAGCTG ACCTTGAGCA TCCTGAGGCA GACTGGTGGA 
TTAGGCATCA GCATAGCAGG AGGGAAGGGA TCTACTCCCT ATAAAGGTGA CGATGAGGGT 
ATTTTCATCT CTAGGGTGTC AGAAGAAGGC CCTGCGGCTC GTGCTGGTGT CAAAGTTGGA 
GACAAACTTC TTGAGGTGAA TGGAGTAGAC CTGCACGGAG CGGAGCATCA TACAGCTGTT 
GAAGCTTTGC GCAATTCAGG AGCAGCCGTG GTCATGACTG TTTTGCGGGA GCGCATGGTG 
GAACCAGAAA ACGCCATCAC CACCACACCG TTGCGGCCAG AAGATGATTA TTTCCCCCGG 
GAGAGACGCT CCAGTGGGCT TCCTTTCCTC CTGGATCCTG ATTGTCCCGC TGTTAGCACT 
GGACCCGCAC AGCGACTAGC CACTTGTCTG ATCCGCAATG ATAAAGGCTT AGGCTTCAGC 
ATTGCTGGTG GGAAAGGGTC AACGCTGTAC AGAGTCGGAG ACACGGGCAT CTTCATCTCT 
CGTATTGCTG AAGGAGGAGC AGCTCACAGA GACAACATCC TGCAAGTGGG AGACAGGGTC 
ATATCAATCA ATGGTGTAGA CATGACAGAG GCCAGGCATG ACCAGGCAGT AGCGCTGCTT 
ACCGGCACCT CCCCCACCAT CACCCTGGTG GTGGATCGGG AACAAAGCAG TGTAGGCGGA 
GCCTCCCCTC GAACCCGACC ACATTCGCCC CCACCTCCAG AGCCCTCTGA CTCCCCTGAG 
CAAGAGGACG GTGGAGACGA GCACCTTGGA AACCATCTCA ACTGCCCCAT GGAAGATGAG 
TACCCTATTG AGGAAGTGAC GCTGATCAAA GCAGGTGGAC CCCTGGGTTT GAGCATTGTA 
GGGGGCAGCG ACCATGCCAG CCATCCATTT GGAATTAATG AACCTGGAGT GTTCATCTCA 
AAGGTCATCC CCAATGGCCT GGCCTCCCAG AGTGGACTTC GCGTGGGTGA TCGTATCTTG 
GAGGTGAACA GCATTGATCT GCGTCATGCC ACACACCAGG AGGCTGTTAG AGCCTTGCTT 
TCCAACAAAC AGGAGATCCG TATGCTTGTG CGCAGAGATC CTTCTCCCCC TGGCATGCAG 
GAAATTGTCA TTCACAAGCA ACCTGGAGAA AAGCTGGGAA TAAGCATTAG GGGCGGAGCT 
AAAGGTCATG CAGGAAACCC CTTTGACCCC ACAGATGAGG GCATCTTTAT TTCCAAGGTG 
AGCTCCAACG GTGCTGCTGC ACGAGATGGC CGACTGCGGG TGGGTATGCG TATTCTTGAG 
GTGGGCAACA ACAGTCTGCT GGGTATGACA CATACAGAAG CAGTGAGGGT CTTGCGTGCT 
TCTGGAGATT CCCTGGTCAT GCTTATTTGT GATGGCTTTG ACCCAAAAAG TGCTTCAACC 
ATAGAGGCAT CTCCTGGTGT TATTGCGAAT CCGTTTGCGG CAGGTATTGT TCGTAAAAAC 
AGTATGGAAA GCATCTCATC AGTCGACAGA GACCTGAGTC CAGAAGAGAT TGACATCATG 
CAGAAGGAGG TAGAAATGGT AAGAGAGACG TCCCAGTGGG AACGAGAGGA GATGGAAAAA 
GTGGAGCGCA TGCGCTTGGA GCGCGAGGAG GCAACTCGCT TGCTGGAGGA GGAAACTGAG 
AGTTTGAGCT CTGGACCACT GAAACTGGAC TATAAGACAC TGGCGGCTCT GCCTACAACT 
AGTCTACAGA AAGTTAATAG ATTTTCTCCC TCCTTTTCCA CATTTGCCAA AATGGAGGCT 
GCCATGCCGG CTGGCCCCGT AGACCCCCTG GGTTTTGAGA AAGTCCATGC TTCTATGTAC 
CATGTAGAAC CTGTTGCCCT CCCTTCATCT CCAATCTGCC CAGCCGCAAG TTCAGCGGCT 
TCAAAACAGC CCCAAGCTGA CTCACGGGAC CTGGTCTTAG ATCTAGGAAT GGCTCAAGAG 
GAGGAGGAAT GTCTTGTGGA CTCCCAACCA ATCTGCTTTA GTGAAAACCC ATTTCTTGTG 
GCTAACCGCA AGGGCAAAGG CTGCCCACCA GAAGAGCGCA TTTTATCAGG GCCACCTGTG 
GGTTACGGCA AGCATGGGCA GCTGCAGCCC TGGTTGTACA GCAAGGCTTC ACCATCTGAG 
CCTTTCAGGA TTGACAGTCC TGTCCGAGAT GCTGCACACT CTCCACACAA CTCACAGCAG 
TCTCACAAAT CTTCCTCTGC GATGCTTGGA AGAGAGGTTC GATATTCAAA CATTCATTTC 
CCTTCAAATG CAAACACCAA GGATAATGCA TCAACTAAAC CTGGTGCCAT CCAGCCTCTC 
TCTCGGGTGC GACCCCCTGT GTCTCCAGCC AGTCAGGATG GCCACAGTAG TCCAAACCCC 
TTCCAGCATG GCCTCTCTCC CATCAACTCT CAGACCACTC CTCGTGCCCC ATCCCCTGAC 
ACACATGAGG AATACCCTAT CAATATTAAG CAGGTTTATA AGGCCTTCGC TGCTGTGCCA 
CACTCCCTGG CCGTACTGGA ACCCCCTCAG GATCTTTATT CTCCACGGAA CAATGTCAGT 
GCAAAACAGC CATCACCTGA GTTTCAGCCT GAGCTGAATA ATGAAGTGTT TATGGATGAG 
TGTGATGGTG AGGGCAGTGG TCCTCGTCCC TCCCTCTTAT CTGACCGCAG CGAGTACAGA 
AGCCTGGCAG CTGTGCCACG CCTGTCTCGC CCTTCAGTGG AGCCAAAGAC CCCTTCGCCT 
CTGGGTAGGC ACAGTCCAGA ACAGCGCTCC TTCAAAGACA GACAAAAGTA TTTCGAGATC 
GATGTGAAGC AACAGACTCC AGACAAACCC AAACCTCGCA TCTCACTGGT GGGAGAAGAT 
GACCTGAAGA AAATGAAGGA GGAAGAAGCA AAGCGAATTG AACAGAGGGC GCGAGAGTTC 
ATGTTGGATG AAGATGAGGA GGAGGAAGAA GAGGATCTTG CCAAGCAAGT GGCACATATG 
AAGGCTCACG GAAAGGTTGT TCTTGATGGT GTGGAGTACA AGGTAGAGAG CTTGGGTTCC 
CCGACTTCAC GGCAGTGTGC CACACCACCA AACTATAGTG CCACACCACC CAGCCACTGC 
GGAAGCTCAG GTCCTTCCTC TATCGACGGT AAAGGAGACT CCCAGCGTAA TTCAGTGGAA 
GACAGTTTCA GACTGGAGCA GAGACCCAAT TCTATGACCG GATTGATCCC AGTTTATCCC 
GGTGAATCTG CGGCTCCCAT CCGGACGGCC AAAGCCGAGC GCAGGCATCA AGATCGATTA 
CGCATGCAGA GCCCAGAGCT CACTGTCGCT TTGGACAAGG AGCTGTCGCC TGCTGAAAAA 
CGAGCCCTGG AGGCAGAGAA GAGAGCCATG TGGAGAGCAG CCAGGATGAA GTCTTTAGAG 
CAGGACGCTT TGAAGGCTCA GATGGTCATT GCCAAGTCTA AAGAGGGCAA GAAGCGAGGA 
ACCCTGGATC AGCTGTCAGA GTCCCCATCC CCTGCACCCA CACCCTCTCC AACACCAATG 
GAAGATATCA GTCCACGGAC AGTGACATCA CCAGGCAGAC TGTCCCTGTC AGAAAAGAAG 
TTTGACTACC GACAATTTGC TGCCATTCCT TCTTCCAAAC CCGTTTACGA CATTCAGTCT 
CCAGGTGCAG CTGATGACGT GCACTTCATG GATGAGTCCT CCAGTAACGC AGTGTCTGTT 
ATTGATCCAG AAGTTCCAGT GGCCGCCACG TCAGCTCTAG AAGAGATGGC TCTCTACAGT 
AACAAACGTA AACTACGCCA GGGCCGCCGC AGCCTGGAGG CTCCTGTGCC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( NM_001007175.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007176.1 Danio rerio scribble planar cell polarity protein (scrib), mRNA.

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CloneID	ODa02667
Clone ID Related Accession (Same CDS sequence)	NM_001007175.1
Accession Version	NM_001007175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB188388.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB188388.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCTTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGGGTCTT CAGAGGAGAG CCACACCACA CCCTAGTGAG 
TTAAAGGTGA TGAAGAATGT AATTGAAGCA AGAAGAAATG AGGCTTATAC AGCCAGACCA 
GATGAAGATC TAGAATCCCC AGATGCTGAG GAGAAACGGT TAAGTGGTCT GTCCAATCAG 
AGTCATGATT CTCAGGCATC CAGTAGCACC ACCTCAGCCA CTTCCCATGA GGACAAGCAG 
GGTATAAACA GTACAGGGGT GGAGCTTAAT GATGGACAGG TTCAAGAAGA GGAGGATCTG 
GATGAGATGG AGGTGGAGTA TACTGAGCCT ACTGTGCACT TTGCTGAAGA GCCCATCATC 
CGTGGTGGTG ATGAGGACGA CGATTATGAT AATGACGATG ATGACGCTGA AAGGAGTGAC 
GAGGTGTCTC AAACGCCATC TTTTCCTGCC GAGAAACAGC GGCTCATACG TAAAGACACA 
CCACACTACA AAAAGCACTT CAAGATCACC AAGCTGCCCA AACCAGAGAC AGTCGCCGCC 
CTGCTGCAAG GCTTCAGTCA TGATGGCCTG TCACCTACTG CTCACACCCC TGAGAACGAG 
AGGGATGGAG AGGATGACGA GGAAGAGGAG GAGGATGAAG ACGAGGAGGA CGATCTCCAT 
ACTCCTTTCC AGCACAGACT AGATGCAGCA GAGCTAGAAG ACATCAGAAT GAGTCAGATG 
AACTCGTCCC TGCAGCCAGT GAAGGGGGTG TCATTTGATC AAGTCAATAA TCTGCTGATT 
GAACCTGCTC GAATTGAGGA GGAAGAGCTG ACCTTGAGCA TCCTGAGGCA GACTGGTGGA 
TTAGGCATCA GCATAGCAGG AGGGAAGGGA TCTACTCCCT ATAAAGGTGA CGATGAGGGT 
ATTTTCATCT CTAGGGTGTC AGAAGAAGGC CCTGCGGCTC GTGCTGGTGT CAAAGTTGGA 
GACAAACTTC TTGAGGTGAA TGGAGTAGAC CTGCACGGAG CGGAGCATCA TACAGCTGTT 
GAAGCTTTGC GCAATTCAGG AGCAGCCGTG GTCATGACTG TTTTGCGGGA GCGCATGGTG 
GAACCAGAAA ACGCCATCAC CACCACACCG TTGCGGCCAG AAGATGATTA TTTCCCCCGG 
GAGAGACGCT CCAGTGGGCT TCCTTTCCTC CTGGATCCTG ATTGTCCCGC TGTTAGCACT 
GGACCCGCAC AGCGACTAGC CACTTGTCTG ATCCGCAATG ATAAAGGCTT AGGCTTCAGC 
ATTGCTGGTG GGAAAGGGTC AACGCTGTAC AGAGTCGGAG ACACGGGCAT CTTCATCTCT 
CGTATTGCTG AAGGAGGAGC AGCTCACAGA GACAACATCC TGCAAGTGGG AGACAGGGTC 
ATATCAATCA ATGGTGTAGA CATGACAGAG GCCAGGCATG ACCAGGCAGT AGCGCTGCTT 
ACCGGCACCT CCCCCACCAT CACCCTGGTG GTGGATCGGG AACAAAGCAG TGTAGGCGGA 
GCCTCCCCTC GAACCCGACC ACATTCGCCC CCACCTCCAG AGCCCTCTGA CTCCCCTGAG 
CAAGAGGACG GTGGAGACGA GCACCTTGGA AACCATCTCA ACTGCCCCAT GGAAGATGAG 
TACCCTATTG AGGAAGTGAC GCTGATCAAA GCAGGTGGAC CCCTGGGTTT GAGCATTGTA 
GGGGGCAGCG ACCATGCCAG CCATCCATTT GGAATTAATG AACCTGGAGT GTTCATCTCA 
AAGGTCATCC CCAATGGCCT GGCCTCCCAG AGTGGACTTC GCGTGGGTGA TCGTATCTTG 
GAGGTGAACA GCATTGATCT GCGTCATGCC ACACACCAGG AGGCTGTTAG AGCCTTGCTT 
TCCAACAAAC AGGAGATCCG TATGCTTGTG CGCAGAGATC CTTCTCCCCC TGGCATGCAG 
GAAATTGTCA TTCACAAGCA ACCTGGAGAA AAGCTGGGAA TAAGCATTAG GGGCGGAGCT 
AAAGGTCATG CAGGAAACCC CTTTGACCCC ACAGATGAGG GCATCTTTAT TTCCAAGGTG 
AGCTCCAACG GTGCTGCTGC ACGAGATGGC CGACTGCGGG TGGGTATGCG TATTCTTGAG 
GTGGGCAACA ACAGTCTGCT GGGTATGACA CATACAGAAG CAGTGAGGGT CTTGCGTGCT 
TCTGGAGATT CCCTGGTCAT GCTTATTTGT GATGGCTTTG ACCCAAAAAG TGCTTCAACC 
ATAGAGGCAT CTCCTGGTGT TATTGCGAAT CCGTTTGCGG CAGGTATTGT TCGTAAAAAC 
AGTATGGAAA GCATCTCATC AGTCGACAGA GACCTGAGTC CAGAAGAGAT TGACATCATG 
CAGAAGGAGG TAGAAATGGT AAGAGAGACG TCCCAGTGGG AACGAGAGGA GATGGAAAAA 
GTGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT GATCTTTATT CTCCACGGAA CAATGTCAGT 
GCAAAACAGC CATCACCTGA GACCCCTTCG CCTCTGGGTA GGCACAGTCC AGAACAGCGC 
TCCTTCAAAG ACAGACAAAA GTATTTCGAG ATCGATGTGA AGCAACAGAC TCCAGACAAA 
CCCAAACCTC GCATCTCACT GGTGGGAGAA GATGACCTGA AGAAAATGAA GGAGGAAGAA 
GCAAAGCGAA TTGAACAGAG GGCGCGAGAG TTCATGTTGG ATGAAGATGA GGAGGAGGAA 
GAAGAGGATC TTGCCAAGCA AGTGGCACAT ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT 
GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT TCCCCGACTT CACGGCAGTG TGCCACACCA 
CCAAACTATA GTGCCACACC ACCCAGCCAC TGCGGAAGCT CAGGTCCTTC CTCTATCGAC 
GGTAAAGGAG ACTCCCAGCG TAATTCAGTG GAAGACAGTT TCAGACTGGA GCAGAGACCC 
AATTCTATGA CCGGATTGAT CCCAGTTTAT CCCGGTGAAT CTGCGGCTCC CATCCGGACG 
GCCAAAGCCG AGCGCAGGCA TCAAGATCGA TTACGCATGC AGAGCCCAGA GCTCACTGTC 
GCTTTGGACA AGGAGCTGTC GCCTGCTGAA AAACGAGCCC TGGAGGCAGA GAAGAGAGCC 
ATGTGGAGAG CAGCCAGGAT GAAGTCTTTA GAGCAGGACG CTTTGAAGGC TCAGATGGTC 
ATTGCCAAGT CTAAAGAGGG CAAGAAGCGA GGAACCCTGG ATCAGCTGTC AGAGTCCCCA 
TCCCCTGCAC CCACACCCTC TCCAACACCA ATGGAAGATA TCAGCCCACG GACAGTGACA 
TCACCAGGCA GACTGTCTCC AGGTGCAGCT GATGACGTGC ACTTCATGGA TGAGTCCTCC 
AGTAACGCAG TGTCTGTTAT TGATCCAGAA GTTCCAGTGG CCGCCACGTC AGCTCTAGAA 
GAGATGGCTC TCTACAGTAA CAAACGTAAA CTACGCCAGG GCCGCCGCAG CCTGGAGGCT 
CCTGTGCCCA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007176.1
CDS	1..5175
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVSLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTDLYSPRNNVSAKQPSPETPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	NM_001007175.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribble planar cell polarity protein (scrib), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007175.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCTTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGGGTCTT CAGAGGAGAG CCACACCACA CCCTAGTGAG 
TTAAAGGTGA TGAAGAATGT AATTGAAGCA AGAAGAAATG AGGCTTATAC AGCCAGACCA 
GATGAAGATC TAGAATCCCC AGATGCTGAG GAGAAACGGT TAAGTGGTCT GTCCAATCAG 
AGTCATGATT CTCAGGCATC CAGTAGCACC ACCTCAGCCA CTTCCCATGA GGACAAGCAG 
GGTATAAACA GTACAGGGGT GGAGCTTAAT GATGGACAGG TTCAAGAAGA GGAGGATCTG 
GATGAGATGG AGGTGGAGTA TACTGAGCCT ACTGTGCACT TTGCTGAAGA GCCCATCATC 
CGTGGTGGTG ATGAGGACGA CGATTATGAT AATGACGATG ATGACGCTGA AAGGAGTGAC 
GAGGTGTCTC AAACGCCATC TTTTCCTGCC GAGAAACAGC GGCTCATACG TAAAGACACA 
CCACACTACA AAAAGCACTT CAAGATCACC AAGCTGCCCA AACCAGAGAC AGTCGCCGCC 
CTGCTGCAAG GCTTCAGTCA TGATGGCCTG TCACCTACTG CTCACACCCC TGAGAACGAG 
AGGGATGGAG AGGATGACGA GGAAGAGGAG GAGGATGAAG ACGAGGAGGA CGATCTCCAT 
ACTCCTTTCC AGCACAGACT AGATGCAGCA GAGCTAGAAG ACATCAGAAT GAGTCAGATG 
AACTCGTCCC TGCAGCCAGT GAAGGGGGTG TCATTTGATC AAGTCAATAA TCTGCTGATT 
GAACCTGCTC GAATTGAGGA GGAAGAGCTG ACCTTGAGCA TCCTGAGGCA GACTGGTGGA 
TTAGGCATCA GCATAGCAGG AGGGAAGGGA TCTACTCCCT ATAAAGGTGA CGATGAGGGT 
ATTTTCATCT CTAGGGTGTC AGAAGAAGGC CCTGCGGCTC GTGCTGGTGT CAAAGTTGGA 
GACAAACTTC TTGAGGTGAA TGGAGTAGAC CTGCACGGAG CGGAGCATCA TACAGCTGTT 
GAAGCTTTGC GCAATTCAGG AGCAGCCGTG GTCATGACTG TTTTGCGGGA GCGCATGGTG 
GAACCAGAAA ACGCCATCAC CACCACACCG TTGCGGCCAG AAGATGATTA TTTCCCCCGG 
GAGAGACGCT CCAGTGGGCT TCCTTTCCTC CTGGATCCTG ATTGTCCCGC TGTTAGCACT 
GGACCCGCAC AGCGACTAGC CACTTGTCTG ATCCGCAATG ATAAAGGCTT AGGCTTCAGC 
ATTGCTGGTG GGAAAGGGTC AACGCTGTAC AGAGTCGGAG ACACGGGCAT CTTCATCTCT 
CGTATTGCTG AAGGAGGAGC AGCTCACAGA GACAACATCC TGCAAGTGGG AGACAGGGTC 
ATATCAATCA ATGGTGTAGA CATGACAGAG GCCAGGCATG ACCAGGCAGT AGCGCTGCTT 
ACCGGCACCT CCCCCACCAT CACCCTGGTG GTGGATCGGG AACAAAGCAG TGTAGGCGGA 
GCCTCCCCTC GAACCCGACC ACATTCGCCC CCACCTCCAG AGCCCTCTGA CTCCCCTGAG 
CAAGAGGACG GTGGAGACGA GCACCTTGGA AACCATCTCA ACTGCCCCAT GGAAGATGAG 
TACCCTATTG AGGAAGTGAC GCTGATCAAA GCAGGTGGAC CCCTGGGTTT GAGCATTGTA 
GGGGGCAGCG ACCATGCCAG CCATCCATTT GGAATTAATG AACCTGGAGT GTTCATCTCA 
AAGGTCATCC CCAATGGCCT GGCCTCCCAG AGTGGACTTC GCGTGGGTGA TCGTATCTTG 
GAGGTGAACA GCATTGATCT GCGTCATGCC ACACACCAGG AGGCTGTTAG AGCCTTGCTT 
TCCAACAAAC AGGAGATCCG TATGCTTGTG CGCAGAGATC CTTCTCCCCC TGGCATGCAG 
GAAATTGTCA TTCACAAGCA ACCTGGAGAA AAGCTGGGAA TAAGCATTAG GGGCGGAGCT 
AAAGGTCATG CAGGAAACCC CTTTGACCCC ACAGATGAGG GCATCTTTAT TTCCAAGGTG 
AGCTCCAACG GTGCTGCTGC ACGAGATGGC CGACTGCGGG TGGGTATGCG TATTCTTGAG 
GTGGGCAACA ACAGTCTGCT GGGTATGACA CATACAGAAG CAGTGAGGGT CTTGCGTGCT 
TCTGGAGATT CCCTGGTCAT GCTTATTTGT GATGGCTTTG ACCCAAAAAG TGCTTCAACC 
ATAGAGGCAT CTCCTGGTGT TATTGCGAAT CCGTTTGCGG CAGGTATTGT TCGTAAAAAC 
AGTATGGAAA GCATCTCATC AGTCGACAGA GACCTGAGTC CAGAAGAGAT TGACATCATG 
CAGAAGGAGG TAGAAATGGT AAGAGAGACG TCCCAGTGGG AACGAGAGGA GATGGAAAAA 
GTGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT GATCTTTATT CTCCACGGAA CAATGTCAGT 
GCAAAACAGC CATCACCTGA GACCCCTTCG CCTCTGGGTA GGCACAGTCC AGAACAGCGC 
TCCTTCAAAG ACAGACAAAA GTATTTCGAG ATCGATGTGA AGCAACAGAC TCCAGACAAA 
CCCAAACCTC GCATCTCACT GGTGGGAGAA GATGACCTGA AGAAAATGAA GGAGGAAGAA 
GCAAAGCGAA TTGAACAGAG GGCGCGAGAG TTCATGTTGG ATGAAGATGA GGAGGAGGAA 
GAAGAGGATC TTGCCAAGCA AGTGGCACAT ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT 
GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT TCCCCGACTT CACGGCAGTG TGCCACACCA 
CCAAACTATA GTGCCACACC ACCCAGCCAC TGCGGAAGCT CAGGTCCTTC CTCTATCGAC 
GGTAAAGGAG ACTCCCAGCG TAATTCAGTG GAAGACAGTT TCAGACTGGA GCAGAGACCC 
AATTCTATGA CCGGATTGAT CCCAGTTTAT CCCGGTGAAT CTGCGGCTCC CATCCGGACG 
GCCAAAGCCG AGCGCAGGCA TCAAGATCGA TTACGCATGC AGAGCCCAGA GCTCACTGTC 
GCTTTGGACA AGGAGCTGTC GCCTGCTGAA AAACGAGCCC TGGAGGCAGA GAAGAGAGCC 
ATGTGGAGAG CAGCCAGGAT GAAGTCTTTA GAGCAGGACG CTTTGAAGGC TCAGATGGTC 
ATTGCCAAGT CTAAAGAGGG CAAGAAGCGA GGAACCCTGG ATCAGCTGTC AGAGTCCCCA 
TCCCCTGCAC CCACACCCTC TCCAACACCA ATGGAAGATA TCAGCCCACG GACAGTGACA 
TCACCAGGCA GACTGTCTCC AGGTGCAGCT GATGACGTGC ACTTCATGGA TGAGTCCTCC 
AGTAACGCAG TGTCTGTTAT TGATCCAGAA GTTCCAGTGG CCGCCACGTC AGCTCTAGAA 
GAGATGGCTC TCTACAGTAA CAAACGTAAA CTACGCCAGG GCCGCCGCAG CCTGGAGGCT 
CCTGTGCCCA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_005166415.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166472.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X9, mRNA.

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CloneID	ODa13339
Clone ID Related Accession (Same CDS sequence)	XM_005166415.3
Accession Version	XM_005166415.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein scribble homolog isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166415.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGACCCCTTC GCCTCTGGGT AGGCACAGTC CAGAACAGCG CTCCTTCAAA 
GACAGACAAA AGTATTTCGA GATCGATGTG AAGCAACAGA CTCCAGACAA ACCCAAACCT 
CGCATCTCAC TGGTGGGAGA AGATGACCTG AAGAAAATGA AGGAGGAAGA AGCAAAGCGA 
ATTGAACAGA GGGCGCGAGA GTTCATGTTG GATGAAGATG AGGAGGAGGA AGAAGAGGAT 
CTTGCCAAGC AAGTGGCACA TATGAAGGCT CACGGAAAGG TTGTTCTTGA TGGTGTGGAG 
TACAAGGTAG AGAGCTTGGG TTCCCCGACT TCACGGCAGT GTGCCACACC ACCAAACTAT 
AGTGCCACAC CACCCAGCCA CTGCGGAAGC TCAGGTCCTT CCTCTATCGA CGGTAAAGGA 
GACTCCCAGC GTAATTCAGT GGAAGACAGT TTCAGACTGG AGCAGAGACC CAATTCTATG 
ACCGGATTGA TCCCAGTTTA TCCCGGTGAA TCTGCGGCTC CCATCCGGAC GGCCAAAGCC 
GAGCGCAGGC ATCAAGATCG ATTACGCATG CAGAGCCCAG AGCTCACTGT CGCTTTGGAC 
AAGGAGCTGT CGCCTGCTGA AAAACGAGCC CTGGAGGCAG AGAAGAGAGC CATGTGGAGA 
GCAGCCAGGA TGAAGTCTTT AGAGCAGGAC GCTTTGAAGG CTCAGATGGT CATTGCCAAG 
TCTAAAGAGG GCAAGAAGCG AGGAACCCTG GATCAGCTGT CAGAGTCCCC ATCCCCTGCA 
CCCACACCCT CTCCAACACC AATGGAAGAT ATCAGTCCAC GGACAGTGAC ATCACCAGGC 
AGACTGTCCC TGTCAGAAAA GAAGTTTGAC TACCGACAAT TTGCTGCCAT TCCTTCTTCC 
AAACCCGTTT ACGACATTCA GTCTCCAGGT GCAGCTGATG ACGTGCACTT CATGGATGAG 
TCCTCCAGTA ACGCAGTGTC TGTTATTGAT CCAGAAGTTC CAGTGGCCGC CACGTCAGCT 
CTAGAAGAGA TGGCTCTCTA CAGTAACAAA CGTAAACTAC GCCAGGGCCG CCGCAGCCTG 
GAGGCTCCTG TGCCCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166472.1
CDS	1125..6545
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPETPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_005166415.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166415.3

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGACCCCTTC GCCTCTGGGT AGGCACAGTC CAGAACAGCG CTCCTTCAAA 
GACAGACAAA AGTATTTCGA GATCGATGTG AAGCAACAGA CTCCAGACAA ACCCAAACCT 
CGCATCTCAC TGGTGGGAGA AGATGACCTG AAGAAAATGA AGGAGGAAGA AGCAAAGCGA 
ATTGAACAGA GGGCGCGAGA GTTCATGTTG GATGAAGATG AGGAGGAGGA AGAAGAGGAT 
CTTGCCAAGC AAGTGGCACA TATGAAGGCT CACGGAAAGG TTGTTCTTGA TGGTGTGGAG 
TACAAGGTAG AGAGCTTGGG TTCCCCGACT TCACGGCAGT GTGCCACACC ACCAAACTAT 
AGTGCCACAC CACCCAGCCA CTGCGGAAGC TCAGGTCCTT CCTCTATCGA CGGTAAAGGA 
GACTCCCAGC GTAATTCAGT GGAAGACAGT TTCAGACTGG AGCAGAGACC CAATTCTATG 
ACCGGATTGA TCCCAGTTTA TCCCGGTGAA TCTGCGGCTC CCATCCGGAC GGCCAAAGCC 
GAGCGCAGGC ATCAAGATCG ATTACGCATG CAGAGCCCAG AGCTCACTGT CGCTTTGGAC 
AAGGAGCTGT CGCCTGCTGA AAAACGAGCC CTGGAGGCAG AGAAGAGAGC CATGTGGAGA 
GCAGCCAGGA TGAAGTCTTT AGAGCAGGAC GCTTTGAAGG CTCAGATGGT CATTGCCAAG 
TCTAAAGAGG GCAAGAAGCG AGGAACCCTG GATCAGCTGT CAGAGTCCCC ATCCCCTGCA 
CCCACACCCT CTCCAACACC AATGGAAGAT ATCAGTCCAC GGACAGTGAC ATCACCAGGC 
AGACTGTCCC TGTCAGAAAA GAAGTTTGAC TACCGACAAT TTGCTGCCAT TCCTTCTTCC 
AAACCCGTTT ACGACATTCA GTCTCCAGGT GCAGCTGATG ACGTGCACTT CATGGATGAG 
TCCTCCAGTA ACGCAGTGTC TGTTATTGAT CCAGAAGTTC CAGTGGCCGC CACGTCAGCT 
CTAGAAGAGA TGGCTCTCTA CAGTAACAAA CGTAAACTAC GCCAGGGCCG CCGCAGCCTG 
GAGGCTCCTG TGCCCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_009303383.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301658.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X1, mRNA.

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CloneID	ODa40217
Clone ID Related Accession (Same CDS sequence)	XM_009303383.2
Accession Version	XM_009303383.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein scribble homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGTTTCAGCC TGAGCTGAAT AATGAAGTGT TTATGGATGA GTGTGATGGT 
GAGGGCAGTG GTCCTCGTCC CTCCCTCTTA TCTGACCGCA GCGAGTACAG AAGCCTGGCA 
GCTGTGCCAC GCCTGTCTCG CCCTTCAGTG GAGCCAAAGA CCCCTTCGCC TCTGGGTAGG 
CACAGTCCAG AACAGCGCTC CTTCAAAGAC AGACAAAAGT ATTTCGAGAT CGATGTGAAG 
CAACAGACTC CAGACAAACC CAAACCTCGC ATCTCACTGG TGGGAGAAGA TGACCTGAAG 
AAAATGAAGG AGGAAGAAGC AAAGCGAATT GAACAGAGGG CGCGAGAGTT CATGTTGGAT 
GAAGATGAGG AGGAGGAAGA AGAGGATCTT GCCAAGCAAG TGGCACATAT GAAGGCTCAC 
GGAAAGGTTG TTCTTGATGG TGTGGAGTAC AAGGTAGAGA GCTTGGGTTC CCCGACTTCA 
CGGCAGTGTG CCACACCACC AAACTATAGT GCCACACCAC CCAGCCACTG CGGAAGCTCA 
GGTCCTTCCT CTATCGACGG TAAAGGAGAC TCCCAGCGTA ATTCAGTGGA AGACAGTTTC 
AGACTGGAGC AGAGACCCAA TTCTATGACC GGATTGATCC CAGTTTATCC CGGTGAATCT 
GCGGCTCCCA TCCGGACGGC CAAAGCCGAG CGCAGGCATC AAGATCGATT ACGCATGCAG 
AGCCCAGAGC TCACTGTCGC TTTGGACAAG GAGCTGTCGC CTGCTGAAAA ACGAGCCCTG 
GAGGCAGAGA AGAGAGCCAT GTGGAGAGCA GCCAGGATGA AGTCTTTAGA GCAGGACGCT 
TTGAAGGCTC AGATGGTCAT TGCCAAGTCT AAAGAGGGCA AGAAGCGAGG AACCCTGGAT 
CAGCTGTCAG AGTCCCCATC CCCTGCACCC ACACCCTCTC CAACACCAAT GGAAGATATC 
AGTCCACGGA CAGTGACATC ACCAGGCAGA CTGTCCCTGT CAGAAAAGAA GTTTGACTAC 
CGACAATTTG CTGCCATTCC TTCTTCCAAA CCCGTTTACG ACATTCAGTC TCCAGGTGCA 
GCTGATGACG TGCACTTCAT GGATGAGTCC TCCAGTAACG CAGTGTCTGT TATTGATCCA 
GAAGTTCCAG TGGCCGCCAC GTCAGCTCTA GAAGAGATGG CTCTCTACAG TAACAAACGT 
AAACTACGCC AGGGCCGCCG CAGCCTGGAG GCTCCTGTGC CCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301658.1
CDS	1131..6698
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_009303383.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303383.2

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGTTTCAGCC TGAGCTGAAT AATGAAGTGT TTATGGATGA GTGTGATGGT 
GAGGGCAGTG GTCCTCGTCC CTCCCTCTTA TCTGACCGCA GCGAGTACAG AAGCCTGGCA 
GCTGTGCCAC GCCTGTCTCG CCCTTCAGTG GAGCCAAAGA CCCCTTCGCC TCTGGGTAGG 
CACAGTCCAG AACAGCGCTC CTTCAAAGAC AGACAAAAGT ATTTCGAGAT CGATGTGAAG 
CAACAGACTC CAGACAAACC CAAACCTCGC ATCTCACTGG TGGGAGAAGA TGACCTGAAG 
AAAATGAAGG AGGAAGAAGC AAAGCGAATT GAACAGAGGG CGCGAGAGTT CATGTTGGAT 
GAAGATGAGG AGGAGGAAGA AGAGGATCTT GCCAAGCAAG TGGCACATAT GAAGGCTCAC 
GGAAAGGTTG TTCTTGATGG TGTGGAGTAC AAGGTAGAGA GCTTGGGTTC CCCGACTTCA 
CGGCAGTGTG CCACACCACC AAACTATAGT GCCACACCAC CCAGCCACTG CGGAAGCTCA 
GGTCCTTCCT CTATCGACGG TAAAGGAGAC TCCCAGCGTA ATTCAGTGGA AGACAGTTTC 
AGACTGGAGC AGAGACCCAA TTCTATGACC GGATTGATCC CAGTTTATCC CGGTGAATCT 
GCGGCTCCCA TCCGGACGGC CAAAGCCGAG CGCAGGCATC AAGATCGATT ACGCATGCAG 
AGCCCAGAGC TCACTGTCGC TTTGGACAAG GAGCTGTCGC CTGCTGAAAA ACGAGCCCTG 
GAGGCAGAGA AGAGAGCCAT GTGGAGAGCA GCCAGGATGA AGTCTTTAGA GCAGGACGCT 
TTGAAGGCTC AGATGGTCAT TGCCAAGTCT AAAGAGGGCA AGAAGCGAGG AACCCTGGAT 
CAGCTGTCAG AGTCCCCATC CCCTGCACCC ACACCCTCTC CAACACCAAT GGAAGATATC 
AGTCCACGGA CAGTGACATC ACCAGGCAGA CTGTCCCTGT CAGAAAAGAA GTTTGACTAC 
CGACAATTTG CTGCCATTCC TTCTTCCAAA CCCGTTTACG ACATTCAGTC TCCAGGTGCA 
GCTGATGACG TGCACTTCAT GGATGAGTCC TCCAGTAACG CAGTGTCTGT TATTGATCCA 
GAAGTTCCAG TGGCCGCCAC GTCAGCTCTA GAAGAGATGG CTCTCTACAG TAACAAACGT 
AAACTACGCC AGGGCCGCCG CAGCCTGGAG GCTCCTGTGC CCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_009303384.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301659.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X2, mRNA.

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CloneID	ODa40218
Clone ID Related Accession (Same CDS sequence)	XM_009303384.2
Accession Version	XM_009303384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein scribble homolog isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGGGTCTT CAGAGGAGAG CCACACCACA CCCTAGTGAG 
TTAAAGGTGA TGAAGAATGT AATTGAAGCA AGAAGAAATG AGGCTTATAC AGCCAGACCA 
GATGAAGATC TAGAATCCCC AGATGCTGAG GAGAAACGGT TAAGTGGTCT GTCCAATCAG 
AGTCATGATT CTCAGGCATC CAGTAGCACC ACCTCAGCCA CTTCCCATGA GGACAAGCAG 
GGTATAAACA GTACAGGGGT GGAGCTTAAT GATGGACAGG TTCAAGAAGA GGAGGATCTG 
GATGAGATGG AGGTGGAGTA TACTGAGCCT ACTGTGCACT TTGCTGAAGA GCCCATCATC 
CGTGGTGGTG ATGAGGACGA CGATTATGAT AATGACGATG ATGACGCTGA AAGGAGTGAC 
GAGGTGTCTC AAACGCCATC TTTTCCTGCC GAGAAACAGC GGCTCATACG TAAAGACACA 
CCACACTACA AAAAGCACTT CAAGATCACC AAGCTGCCCA AACCAGAGAC AGTCGCCGCC 
CTGCTGCAAG GCTTCAGTCA TGATGGCCTG TCACCTACTG CTCACACCCC TGAGAACGAG 
AGGGATGGAG AGGATGACGA GGAAGAGGAG GAGGATGAAG ACGAGGAGGA CGATCTCCAT 
ACTCCTTTCC AGCACAGACT AGATGCAGCA GAGCTAGAAG ACATCAGAAT GAGTCAGATG 
AACTCGTCCC TGCAGCCAGT GAAGGGGGTG TCATTTGATC AAGTCAATAA TCTGCTGATT 
GAACCTGCTC GAATTGAGGA GGAAGAGCTG ACCTTGAGCA TCCTGAGGCA GACTGGTGGA 
TTAGGCATCA GCATAGCAGG AGGGAAGGGA TCTACTCCCT ATAAAGGTGA CGATGAGGGT 
ATTTTCATCT CTAGGGTGTC AGAAGAAGGC CCTGCGGCTC GTGCTGGTGT CAAAGTTGGA 
GACAAACTTC TTGAGGTGAA TGGAGTAGAC CTGCACGGAG CGGAGCATCA TACAGCTGTT 
GAAGCTTTGC GCAATTCAGG AGCAGCCGTG GTCATGACTG TTTTGCGGGA GCGCATGGTG 
GAACCAGAAA ACGCCATCAC CACCACACCG TTGCGGCCAG AAGATGATTA TTTCCCCCGG 
GAGAGACGCT CCAGTGGGCT TCCTTTCCTC CTGGATCCTG ATTGTCCCGC TGTTAGCACT 
GGACCCGCAC AGCGACTAGC CACTTGTCTG ATCCGCAATG ATAAAGGCTT AGGCTTCAGC 
ATTGCTGGTG GGAAAGGGTC AACGCTGTAC AGAGTCGGAG ACACGGGCAT CTTCATCTCT 
CGTATTGCTG AAGGAGGAGC AGCTCACAGA GACAACATCC TGCAAGTGGG AGACAGGGTC 
ATATCAATCA ATGGTGTAGA CATGACAGAG GCCAGGCATG ACCAGGCAGT AGCGCTGCTT 
ACCGGCACCT CCCCCACCAT CACCCTGGTG GTGGATCGGG AACAAAGCAG TGTAGGCGGA 
GCCTCCCCTC GAACCCGACC ACATTCGCCC CCACCTCCAG AGCCCTCTGA CTCCCCTGAG 
CAAGAGGACG GTGGAGACGA GCACCTTGGA AACCATCTCA ACTGCCCCAT GGAAGATGAG 
TACCCTATTG AGGAAGTGAC GCTGATCAAA GCAGGTGGAC CCCTGGGTTT GAGCATTGTA 
GGGGGCAGCG ACCATGCCAG CCATCCATTT GGAATTAATG AACCTGGAGT GTTCATCTCA 
AAGGTCATCC CCAATGGCCT GGCCTCCCAG AGTGGACTTC GCGTGGGTGA TCGTATCTTG 
GAGGTGAACA GCATTGATCT GCGTCATGCC ACACACCAGG AGGCTGTTAG AGCCTTGCTT 
TCCAACAAAC AGGAGATCCG TATGCTTGTG CGCAGAGATC CTTCTCCCCC TGGCATGCAG 
GAAATTGTCA TTCACAAGCA ACCTGGAGAA AAGCTGGGAA TAAGCATTAG GGGCGGAGCT 
AAAGGTCATG CAGGAAACCC CTTTGACCCC ACAGATGAGG GCATCTTTAT TTCCAAGGTG 
AGCTCCAACG GTGCTGCTGC ACGAGATGGC CGACTGCGGG TGGGTATGCG TATTCTTGAG 
GTGGGCAACA ACAGTCTGCT GGGTATGACA CATACAGAAG CAGTGAGGGT CTTGCGTGCT 
TCTGGAGATT CCCTGGTCAT GCTTATTTGT GATGGCTTTG ACCCAAAAAG TGCTTCAACC 
ATAGAGGCAT CTCCTGGTGT TATTGCGAAT CCGTTTGCGG CAGGTATTGT TCGTAAAAAC 
AGTATGGAAA GCATCTCATC AGTCGACAGA GACCTGAGTC CAGAAGAGAT TGACATCATG 
CAGAAGGAGG TAGAAATGGT AAGAGAGACG TCCCAGTGGG AACGAGAGGA GATGGAAAAA 
GTGGAGCGCA TGCGCTTGGA GCGCGAGGAG GCAACTCGCT TGCTGGAGGA GGAAACTGAG 
AGTTTGAGCT CTGGACCACT GAAACTGGAC TATAAGACAC TGGCGGCTCT GCCTACAACT 
AGTCTACAGA AAGTTAATAG AGCTTCACCA TCTGAGCCTT TCAGGATTGA CAGTCCTGTC 
CGAGATGCTG CACACTCTCC ACACAACTCA CAGTCAAACA TTCATTTCCC TTCAAATGCA 
AACACCAAGG ATAATGCATC AACTAAACCT GGTGCCATCC AGCCTCTCTC TCGGGTGCGA 
CCCCCTGTGT CTCCAGCCAG TCAGGATGGC CACAGTAGTC CAAACCCCTT CCAGCATGGC 
CTCTCTCCCA TCAACTCTCA GACCACTCCT CGTGCCCCAT CCCCTGACAC ACATGAGGAA 
TACCCTATCA ATATTAAGCA GGTTTATAAG GCCTTCGCTG CTGTGCCACA CTCCCTGGCC 
GTACTGGAAC CCCCTCAGGA TCTTTATTCT CCACGGAACA ATGTCAGTGC AAAACAGCCA 
TCACCTGAGT TTCAGCCTGA GCTGAATAAT GAAGTGTTTA TGGATGAGTG TGATGGTGAG 
GGCAGTGGTC CTCGTCCCTC CCTCTTATCT GACCGCAGCG AGTACAGAAG CCTGGCAGCT 
GTGCCACGCC TGTCTCGCCC TTCAGTGGAG CCAAAGACCC CTTCGCCTCT GGGTAGGCAC 
AGTCCAGAAC AGCGCTCCTT CAAAGACAGA CAAAAGTATT TCGAGATCGA TGTGAAGCAA 
CAGACTCCAG ACAAACCCAA ACCTCGCATC TCACTGGTGG GAGAAGATGA CCTGAAGAAA 
ATGAAGGAGG AAGAAGCAAA GCGAATTGAA CAGAGGGCGC GAGAGTTCAT GTTGGATGAA 
GATGAGGAGG AGGAAGAAGA GGATCTTGCC AAGCAAGTGG CACATATGAA GGCTCACGGA 
AAGGTTGTTC TTGATGGTGT GGAGTACAAG GTAGAGAGCT TGGGTTCCCC GACTTCACGG 
CAGTGTGCCA CACCACCAAA CTATAGTGCC ACACCACCCA GCCACTGCGG AAGCTCAGGT 
CCTTCCTCTA TCGACGGTAA AGGAGACTCC CAGCGTAATT CAGTGGAAGA CAGTTTCAGA 
CTGGAGCAGA GACCCAATTC TATGACCGGA TTGATCCCAG TTTATCCCGG TGAATCTGCG 
GCTCCCATCC GGACGGCCAA AGCCGAGCGC AGGCATCAAG ATCGATTACG CATGCAGAGC 
CCAGAGCTCA CTGTCGCTTT GGACAAGGAG CTGTCGCCTG CTGAAAAACG AGCCCTGGAG 
GCAGAGAAGA GAGCCATGTG GAGAGCAGCC AGGATGAAGT CTTTAGAGCA GGACGCTTTG 
AAGGCTCAGA TGGTCATTGC CAAGTCTAAA GAGGGCAAGA AGCGAGGAAC CCTGGATCAG 
CTGTCAGAGT CCCCATCCCC TGCACCCACA CCCTCTCCAA CACCAATGGA AGATATCAGT 
CCACGGACAG TGACATCACC AGGCAGACTG TCCCTGTCAG AAAAGAAGTT TGACTACCGA 
CAATTTGCTG CCATTCCTTC TTCCAAACCC GTTTACGACA TTCAGTCTCC AGGTGCAGCT 
GATGACGTGC ACTTCATGGA TGAGTCCTCC AGTAACGCAG TGTCTGTTAT TGATCCAGAA 
GTTCCAGTGG CCGCCACGTC AGCTCTAGAA GAGATGGCTC TCTACAGTAA CAAACGTAAA 
CTACGCCAGG GCCGCCGCAG CCTGGAGGCT CCTGTGCCCA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301659.1
CDS	1128..6692
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_009303384.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303384.2

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGGGTCTT CAGAGGAGAG CCACACCACA CCCTAGTGAG 
TTAAAGGTGA TGAAGAATGT AATTGAAGCA AGAAGAAATG AGGCTTATAC AGCCAGACCA 
GATGAAGATC TAGAATCCCC AGATGCTGAG GAGAAACGGT TAAGTGGTCT GTCCAATCAG 
AGTCATGATT CTCAGGCATC CAGTAGCACC ACCTCAGCCA CTTCCCATGA GGACAAGCAG 
GGTATAAACA GTACAGGGGT GGAGCTTAAT GATGGACAGG TTCAAGAAGA GGAGGATCTG 
GATGAGATGG AGGTGGAGTA TACTGAGCCT ACTGTGCACT TTGCTGAAGA GCCCATCATC 
CGTGGTGGTG ATGAGGACGA CGATTATGAT AATGACGATG ATGACGCTGA AAGGAGTGAC 
GAGGTGTCTC AAACGCCATC TTTTCCTGCC GAGAAACAGC GGCTCATACG TAAAGACACA 
CCACACTACA AAAAGCACTT CAAGATCACC AAGCTGCCCA AACCAGAGAC AGTCGCCGCC 
CTGCTGCAAG GCTTCAGTCA TGATGGCCTG TCACCTACTG CTCACACCCC TGAGAACGAG 
AGGGATGGAG AGGATGACGA GGAAGAGGAG GAGGATGAAG ACGAGGAGGA CGATCTCCAT 
ACTCCTTTCC AGCACAGACT AGATGCAGCA GAGCTAGAAG ACATCAGAAT GAGTCAGATG 
AACTCGTCCC TGCAGCCAGT GAAGGGGGTG TCATTTGATC AAGTCAATAA TCTGCTGATT 
GAACCTGCTC GAATTGAGGA GGAAGAGCTG ACCTTGAGCA TCCTGAGGCA GACTGGTGGA 
TTAGGCATCA GCATAGCAGG AGGGAAGGGA TCTACTCCCT ATAAAGGTGA CGATGAGGGT 
ATTTTCATCT CTAGGGTGTC AGAAGAAGGC CCTGCGGCTC GTGCTGGTGT CAAAGTTGGA 
GACAAACTTC TTGAGGTGAA TGGAGTAGAC CTGCACGGAG CGGAGCATCA TACAGCTGTT 
GAAGCTTTGC GCAATTCAGG AGCAGCCGTG GTCATGACTG TTTTGCGGGA GCGCATGGTG 
GAACCAGAAA ACGCCATCAC CACCACACCG TTGCGGCCAG AAGATGATTA TTTCCCCCGG 
GAGAGACGCT CCAGTGGGCT TCCTTTCCTC CTGGATCCTG ATTGTCCCGC TGTTAGCACT 
GGACCCGCAC AGCGACTAGC CACTTGTCTG ATCCGCAATG ATAAAGGCTT AGGCTTCAGC 
ATTGCTGGTG GGAAAGGGTC AACGCTGTAC AGAGTCGGAG ACACGGGCAT CTTCATCTCT 
CGTATTGCTG AAGGAGGAGC AGCTCACAGA GACAACATCC TGCAAGTGGG AGACAGGGTC 
ATATCAATCA ATGGTGTAGA CATGACAGAG GCCAGGCATG ACCAGGCAGT AGCGCTGCTT 
ACCGGCACCT CCCCCACCAT CACCCTGGTG GTGGATCGGG AACAAAGCAG TGTAGGCGGA 
GCCTCCCCTC GAACCCGACC ACATTCGCCC CCACCTCCAG AGCCCTCTGA CTCCCCTGAG 
CAAGAGGACG GTGGAGACGA GCACCTTGGA AACCATCTCA ACTGCCCCAT GGAAGATGAG 
TACCCTATTG AGGAAGTGAC GCTGATCAAA GCAGGTGGAC CCCTGGGTTT GAGCATTGTA 
GGGGGCAGCG ACCATGCCAG CCATCCATTT GGAATTAATG AACCTGGAGT GTTCATCTCA 
AAGGTCATCC CCAATGGCCT GGCCTCCCAG AGTGGACTTC GCGTGGGTGA TCGTATCTTG 
GAGGTGAACA GCATTGATCT GCGTCATGCC ACACACCAGG AGGCTGTTAG AGCCTTGCTT 
TCCAACAAAC AGGAGATCCG TATGCTTGTG CGCAGAGATC CTTCTCCCCC TGGCATGCAG 
GAAATTGTCA TTCACAAGCA ACCTGGAGAA AAGCTGGGAA TAAGCATTAG GGGCGGAGCT 
AAAGGTCATG CAGGAAACCC CTTTGACCCC ACAGATGAGG GCATCTTTAT TTCCAAGGTG 
AGCTCCAACG GTGCTGCTGC ACGAGATGGC CGACTGCGGG TGGGTATGCG TATTCTTGAG 
GTGGGCAACA ACAGTCTGCT GGGTATGACA CATACAGAAG CAGTGAGGGT CTTGCGTGCT 
TCTGGAGATT CCCTGGTCAT GCTTATTTGT GATGGCTTTG ACCCAAAAAG TGCTTCAACC 
ATAGAGGCAT CTCCTGGTGT TATTGCGAAT CCGTTTGCGG CAGGTATTGT TCGTAAAAAC 
AGTATGGAAA GCATCTCATC AGTCGACAGA GACCTGAGTC CAGAAGAGAT TGACATCATG 
CAGAAGGAGG TAGAAATGGT AAGAGAGACG TCCCAGTGGG AACGAGAGGA GATGGAAAAA 
GTGGAGCGCA TGCGCTTGGA GCGCGAGGAG GCAACTCGCT TGCTGGAGGA GGAAACTGAG 
AGTTTGAGCT CTGGACCACT GAAACTGGAC TATAAGACAC TGGCGGCTCT GCCTACAACT 
AGTCTACAGA AAGTTAATAG AGCTTCACCA TCTGAGCCTT TCAGGATTGA CAGTCCTGTC 
CGAGATGCTG CACACTCTCC ACACAACTCA CAGTCAAACA TTCATTTCCC TTCAAATGCA 
AACACCAAGG ATAATGCATC AACTAAACCT GGTGCCATCC AGCCTCTCTC TCGGGTGCGA 
CCCCCTGTGT CTCCAGCCAG TCAGGATGGC CACAGTAGTC CAAACCCCTT CCAGCATGGC 
CTCTCTCCCA TCAACTCTCA GACCACTCCT CGTGCCCCAT CCCCTGACAC ACATGAGGAA 
TACCCTATCA ATATTAAGCA GGTTTATAAG GCCTTCGCTG CTGTGCCACA CTCCCTGGCC 
GTACTGGAAC CCCCTCAGGA TCTTTATTCT CCACGGAACA ATGTCAGTGC AAAACAGCCA 
TCACCTGAGT TTCAGCCTGA GCTGAATAAT GAAGTGTTTA TGGATGAGTG TGATGGTGAG 
GGCAGTGGTC CTCGTCCCTC CCTCTTATCT GACCGCAGCG AGTACAGAAG CCTGGCAGCT 
GTGCCACGCC TGTCTCGCCC TTCAGTGGAG CCAAAGACCC CTTCGCCTCT GGGTAGGCAC 
AGTCCAGAAC AGCGCTCCTT CAAAGACAGA CAAAAGTATT TCGAGATCGA TGTGAAGCAA 
CAGACTCCAG ACAAACCCAA ACCTCGCATC TCACTGGTGG GAGAAGATGA CCTGAAGAAA 
ATGAAGGAGG AAGAAGCAAA GCGAATTGAA CAGAGGGCGC GAGAGTTCAT GTTGGATGAA 
GATGAGGAGG AGGAAGAAGA GGATCTTGCC AAGCAAGTGG CACATATGAA GGCTCACGGA 
AAGGTTGTTC TTGATGGTGT GGAGTACAAG GTAGAGAGCT TGGGTTCCCC GACTTCACGG 
CAGTGTGCCA CACCACCAAA CTATAGTGCC ACACCACCCA GCCACTGCGG AAGCTCAGGT 
CCTTCCTCTA TCGACGGTAA AGGAGACTCC CAGCGTAATT CAGTGGAAGA CAGTTTCAGA 
CTGGAGCAGA GACCCAATTC TATGACCGGA TTGATCCCAG TTTATCCCGG TGAATCTGCG 
GCTCCCATCC GGACGGCCAA AGCCGAGCGC AGGCATCAAG ATCGATTACG CATGCAGAGC 
CCAGAGCTCA CTGTCGCTTT GGACAAGGAG CTGTCGCCTG CTGAAAAACG AGCCCTGGAG 
GCAGAGAAGA GAGCCATGTG GAGAGCAGCC AGGATGAAGT CTTTAGAGCA GGACGCTTTG 
AAGGCTCAGA TGGTCATTGC CAAGTCTAAA GAGGGCAAGA AGCGAGGAAC CCTGGATCAG 
CTGTCAGAGT CCCCATCCCC TGCACCCACA CCCTCTCCAA CACCAATGGA AGATATCAGT 
CCACGGACAG TGACATCACC AGGCAGACTG TCCCTGTCAG AAAAGAAGTT TGACTACCGA 
CAATTTGCTG CCATTCCTTC TTCCAAACCC GTTTACGACA TTCAGTCTCC AGGTGCAGCT 
GATGACGTGC ACTTCATGGA TGAGTCCTCC AGTAACGCAG TGTCTGTTAT TGATCCAGAA 
GTTCCAGTGG CCGCCACGTC AGCTCTAGAA GAGATGGCTC TCTACAGTAA CAAACGTAAA 
CTACGCCAGG GCCGCCGCAG CCTGGAGGCT CCTGTGCCCA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_009303385.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301660.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X3, mRNA.

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CloneID	ODa40219
Clone ID Related Accession (Same CDS sequence)	XM_009303385.2
Accession Version	XM_009303385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein scribble homolog isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGAT GAAGTCTTTA 
GAGCAGGACG CTTTGAAGGC TCAGATGGTC ATTGCCAAGT CTAAAGAGGG CAAGAAGCGA 
GGAACCCTGG ATCAGCTGTC AGAGTCCCCA TCCCCTGCAC CCACACCCTC TCCAACACCA 
ATGGAAGATA TCAGTCCACG GACAGTGACA TCACCAGGCA GACTGTCCCT GTCAGAAAAG 
AAGTTTGACT ACCGACAATT TGCTGCCATT CCTTCTTCCA AACCCGTTTA CGACATTCAG 
TCTCCAGGTG CAGCTGATGA CGTGCACTTC ATGGATGAGT CCTCCAGTAA CGCAGTGTCT 
GTTATTGATC CAGAAGTTCC AGTGGCCGCC ACGTCAGCTC TAGAAGAGAT GGCTCTCTAC 
AGTAACAAAC GTAAACTACG CCAGGGCCGC CGCAGCCTGG AGGCTCCTGT GCCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301660.1
CDS	1133..6652
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_009303385.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303385.2

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGAT GAAGTCTTTA 
GAGCAGGACG CTTTGAAGGC TCAGATGGTC ATTGCCAAGT CTAAAGAGGG CAAGAAGCGA 
GGAACCCTGG ATCAGCTGTC AGAGTCCCCA TCCCCTGCAC CCACACCCTC TCCAACACCA 
ATGGAAGATA TCAGTCCACG GACAGTGACA TCACCAGGCA GACTGTCCCT GTCAGAAAAG 
AAGTTTGACT ACCGACAATT TGCTGCCATT CCTTCTTCCA AACCCGTTTA CGACATTCAG 
TCTCCAGGTG CAGCTGATGA CGTGCACTTC ATGGATGAGT CCTCCAGTAA CGCAGTGTCT 
GTTATTGATC CAGAAGTTCC AGTGGCCGCC ACGTCAGCTC TAGAAGAGAT GGCTCTCTAC 
AGTAACAAAC GTAAACTACG CCAGGGCCGC CGCAGCCTGG AGGCTCCTGT GCCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_009303386.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301661.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X4, mRNA.

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CloneID	ODa40220
Clone ID Related Accession (Same CDS sequence)	XM_009303386.2
Accession Version	XM_009303386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein scribble homolog isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGAGTT TGAGCTCTGG ACCACTGAAA CTGGACTATA AGACACTGGC GGCTCTGCCT 
ACAACTAGTC TACAGAAAGT TAATAGAGCT TCACCATCTG AGCCTTTCAG GATTGACAGT 
CCTGTCCGAG ATGCTGCACA CTCTCCACAC AACTCACAGT CAAACATTCA TTTCCCTTCA 
AATGCAAACA CCAAGGATAA TGCATCAACT AAACCTGGTG CCATCCAGCC TCTCTCTCGG 
GTGCGACCCC CTGTGTCTCC AGCCAGTCAG GATGGCCACA GTAGTCCAAA CCCCTTCCAG 
CATGGCCTCT CTCCCATCAA CTCTCAGACC ACTCCTCGTG CCCCATCCCC TGACACACAT 
GAGGAATACC CTATCAATAT TAAGCAGGTT TATAAGGCCT TCGCTGCTGT GCCACACTCC 
CTGGCCGTAC TGGAACCCCC TCAGGATCTT TATTCTCCAC GGAACAATGT CAGTGCAAAA 
CAGCCATCAC CTGAGTTTCA GCCTGAGCTG AATAATGAAG TGTTTATGGA TGAGTGTGAT 
GGTGAGGGCA GTGGTCCTCG TCCCTCCCTC TTATCTGACC GCAGCGAGTA CAGAAGCCTG 
GCAGCTGTGC CACGCCTGTC TCGCCCTTCA GTGGAGCCAA AGACCCCTTC GCCTCTGGGT 
AGGCACAGTC CAGAACAGCG CTCCTTCAAA GACAGACAAA AGTATTTCGA GATCGATGTG 
AAGCAACAGA CTCCAGACAA ACCCAAACCT CGCATCTCAC TGGTGGGAGA AGATGACCTG 
AAGAAAATGA AGGAGGAAGA AGCAAAGCGA ATTGAACAGA GGGCGCGAGA GTTCATGTTG 
GATGAAGATG AGGAGGAGGA AGAAGAGGAT CTTGCCAAGC AAGTGGCACA TATGAAGGCT 
CACGGAAAGG TTGTTCTTGA TGGTGTGGAG TACAAGGTAG AGAGCTTGGG TTCCCCGACT 
TCACGGCAGT GTGCCACACC ACCAAACTAT AGTGCCACAC CACCCAGCCA CTGCGGAAGC 
TCAGGTCCTT CCTCTATCGA CGGTAAAGGA GACTCCCAGC GTAATTCAGT GGAAGACAGT 
TTCAGACTGG AGCAGAGACC CAATTCTATG ACCGGATTGA TCCCAGTTTA TCCCGGTGAA 
TCTGCGGCTC CCATCCGGAC GGCCAAAGCC GAGCGCAGGC ATCAAGATCG ATTACGCATG 
CAGAGCCCAG AGCTCACTGT CGCTTTGGAC AAGGAGCTGT CGCCTGCTGA AAAACGAGCC 
CTGGAGGCAG AGAAGAGAGC CATGTGGAGA GCAGCCAGGA TGAAGTCTTT AGAGCAGGAC 
GCTTTGAAGG CTCAGATGGT CATTGCCAAG TCTAAAGAGG GCAAGAAGCG AGGAACCCTG 
GATCAGCTGT CAGAGTCCCC ATCCCCTGCA CCCACACCCT CTCCAACACC AATGGAAGAT 
ATCAGTCCAC GGACAGTGAC ATCACCAGGC AGACTGTCCC TGTCAGAAAA GAAGTTTGAC 
TACCGACAAT TTGCTGCCAT TCCTTCTTCC AAACCCGTTT ACGACATTCA GTCTCCAGGT 
GCAGCTGATG ACGTGCACTT CATGGATGAG TCCTCCAGTA ACGCAGTGTC TGTTATTGAT 
CCAGAAGTTC CAGTGGCCGC CACGTCAGCT CTAGAAGAGA TGGCTCTCTA CAGTAACAAA 
CGTAAACTAC GCCAGGGCCG CCGCAGCCTG GAGGCTCCTG TGCCCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301661.1
CDS	1130..6640
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVSLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_009303386.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303386.2

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGAGTT TGAGCTCTGG ACCACTGAAA CTGGACTATA AGACACTGGC GGCTCTGCCT 
ACAACTAGTC TACAGAAAGT TAATAGAGCT TCACCATCTG AGCCTTTCAG GATTGACAGT 
CCTGTCCGAG ATGCTGCACA CTCTCCACAC AACTCACAGT CAAACATTCA TTTCCCTTCA 
AATGCAAACA CCAAGGATAA TGCATCAACT AAACCTGGTG CCATCCAGCC TCTCTCTCGG 
GTGCGACCCC CTGTGTCTCC AGCCAGTCAG GATGGCCACA GTAGTCCAAA CCCCTTCCAG 
CATGGCCTCT CTCCCATCAA CTCTCAGACC ACTCCTCGTG CCCCATCCCC TGACACACAT 
GAGGAATACC CTATCAATAT TAAGCAGGTT TATAAGGCCT TCGCTGCTGT GCCACACTCC 
CTGGCCGTAC TGGAACCCCC TCAGGATCTT TATTCTCCAC GGAACAATGT CAGTGCAAAA 
CAGCCATCAC CTGAGTTTCA GCCTGAGCTG AATAATGAAG TGTTTATGGA TGAGTGTGAT 
GGTGAGGGCA GTGGTCCTCG TCCCTCCCTC TTATCTGACC GCAGCGAGTA CAGAAGCCTG 
GCAGCTGTGC CACGCCTGTC TCGCCCTTCA GTGGAGCCAA AGACCCCTTC GCCTCTGGGT 
AGGCACAGTC CAGAACAGCG CTCCTTCAAA GACAGACAAA AGTATTTCGA GATCGATGTG 
AAGCAACAGA CTCCAGACAA ACCCAAACCT CGCATCTCAC TGGTGGGAGA AGATGACCTG 
AAGAAAATGA AGGAGGAAGA AGCAAAGCGA ATTGAACAGA GGGCGCGAGA GTTCATGTTG 
GATGAAGATG AGGAGGAGGA AGAAGAGGAT CTTGCCAAGC AAGTGGCACA TATGAAGGCT 
CACGGAAAGG TTGTTCTTGA TGGTGTGGAG TACAAGGTAG AGAGCTTGGG TTCCCCGACT 
TCACGGCAGT GTGCCACACC ACCAAACTAT AGTGCCACAC CACCCAGCCA CTGCGGAAGC 
TCAGGTCCTT CCTCTATCGA CGGTAAAGGA GACTCCCAGC GTAATTCAGT GGAAGACAGT 
TTCAGACTGG AGCAGAGACC CAATTCTATG ACCGGATTGA TCCCAGTTTA TCCCGGTGAA 
TCTGCGGCTC CCATCCGGAC GGCCAAAGCC GAGCGCAGGC ATCAAGATCG ATTACGCATG 
CAGAGCCCAG AGCTCACTGT CGCTTTGGAC AAGGAGCTGT CGCCTGCTGA AAAACGAGCC 
CTGGAGGCAG AGAAGAGAGC CATGTGGAGA GCAGCCAGGA TGAAGTCTTT AGAGCAGGAC 
GCTTTGAAGG CTCAGATGGT CATTGCCAAG TCTAAAGAGG GCAAGAAGCG AGGAACCCTG 
GATCAGCTGT CAGAGTCCCC ATCCCCTGCA CCCACACCCT CTCCAACACC AATGGAAGAT 
ATCAGTCCAC GGACAGTGAC ATCACCAGGC AGACTGTCCC TGTCAGAAAA GAAGTTTGAC 
TACCGACAAT TTGCTGCCAT TCCTTCTTCC AAACCCGTTT ACGACATTCA GTCTCCAGGT 
GCAGCTGATG ACGTGCACTT CATGGATGAG TCCTCCAGTA ACGCAGTGTC TGTTATTGAT 
CCAGAAGTTC CAGTGGCCGC CACGTCAGCT CTAGAAGAGA TGGCTCTCTA CAGTAACAAA 
CGTAAACTAC GCCAGGGCCG CCGCAGCCTG GAGGCTCCTG TGCCCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_009303387.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301662.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X5, mRNA.

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CloneID	ODa40221
Clone ID Related Accession (Same CDS sequence)	XM_009303387.2
Accession Version	XM_009303387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein scribble homolog isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGCTG ACCTTGAGCA TCCTGAGGCA GACTGGTGGA 
TTAGGCATCA GCATAGCAGG AGGGAAGGGA TCTACTCCCT ATAAAGGTGA CGATGAGGGT 
ATTTTCATCT CTAGGGTGTC AGAAGAAGGC CCTGCGGCTC GTGCTGGTGT CAAAGTTGGA 
GACAAACTTC TTGAGGTGAA TGGAGTAGAC CTGCACGGAG CGGAGCATCA TACAGCTGTT 
GAAGCTTTGC GCAATTCAGG AGCAGCCGTG GTCATGACTG TTTTGCGGGA GCGCATGGTG 
GAACCAGAAA ACGCCATCAC CACCACACCG TTGCGGCCAG AAGATGATTA TTTCCCCCGG 
GAGAGACGCT CCAGTGGGCT TCCTTTCCTC CTGGATCCTG ATTGTCCCGC TGTTAGCACT 
GGACCCGCAC AGCGACTAGC CACTTGTCTG ATCCGCAATG ATAAAGGCTT AGGCTTCAGC 
ATTGCTGGTG GGAAAGGGTC AACGCTGTAC AGAGTCGGAG ACACGGGCAT CTTCATCTCT 
CGTATTGCTG AAGGAGGAGC AGCTCACAGA GACAACATCC TGCAAGTGGG AGACAGGGTC 
ATATCAATCA ATGGTGTAGA CATGACAGAG GCCAGGCATG ACCAGGCAGT AGCGCTGCTT 
ACCGGCACCT CCCCCACCAT CACCCTGGTG GTGGATCGGG AACAAAGCAG TGTAGGCGGA 
GCCTCCCCTC GAACCCGACC ACATTCGCCC CCACCTCCAG AGCCCTCTGA CTCCCCTGAG 
CAAGAGGACG GTGGAGACGA GCACCTTGGA AACCATCTCA ACTGCCCCAT GGAAGATGAG 
TACCCTATTG AGGAAGTGAC GCTGATCAAA GCAGGTGGAC CCCTGGGTTT GAGCATTGTA 
GGGGGCAGCG ACCATGCCAG CCATCCATTT GGAATTAATG AACCTGGAGT GTTCATCTCA 
AAGGTCATCC CCAATGGCCT GGCCTCCCAG AGTGGACTTC GCGTGGGTGA TCGTATCTTG 
GAGGTGAACA GCATTGATCT GCGTCATGCC ACACACCAGG AGGCTGTTAG AGCCTTGCTT 
TCCAACAAAC AGGAGATCCG TATGCTTGTG CGCAGAGATC CTTCTCCCCC TGGCATGCAG 
GAAATTGTCA TTCACAAGCA ACCTGGAGAA AAGCTGGGAA TAAGCATTAG GGGCGGAGCT 
AAAGGTCATG CAGGAAACCC CTTTGACCCC ACAGATGAGG GCATCTTTAT TTCCAAGGTG 
AGCTCCAACG GTGCTGCTGC ACGAGATGGC CGACTGCGGG TGGGTATGCG TATTCTTGAG 
GTGGGCAACA ACAGTCTGCT GGGTATGACA CATACAGAAG CAGTGAGGGT CTTGCGTGCT 
TCTGGAGATT CCCTGGTCAT GCTTATTTGT GATGGCTTTG ACCCAAAAAG TGCTTCAACC 
ATAGAGGCAT CTCCTGGTGT TATTGCGAAT CCGTTTGCGG CAGGTATTGT TCGTAAAAAC 
AGTATGGAAA GCATCTCATC AGTCGACAGA GACCTGAGTC CAGAAGAGAT TGACATCATG 
CAGAAGGAGG TAGAAATGGT AAGAGAGACG TCCCAGTGGG AACGAGAGGA GATGGAAAAA 
GTGGAGCGCA TGCGCTTGGA GCGCGAGGAG GCAACTCGCT TGCTGGAGGA GGAAACTGAG 
AGTTTGAGCT CTGGACCACT GAAACTGGAC TATAAGACAC TGGCGGCTCT GCCTACAACT 
AGTCTACAGA AAGTTAATAG AGCTTCACCA TCTGAGCCTT TCAGGATTGA CAGTCCTGTC 
CGAGATGCTG CACACTCTCC ACACAACTCA CAGTCAAACA TTCATTTCCC TTCAAATGCA 
AACACCAAGG ATAATGCATC AACTAAACCT GGTGCCATCC AGCCTCTCTC TCGGGTGCGA 
CCCCCTGTGT CTCCAGCCAG TCAGGATGGC CACAGTAGTC CAAACCCCTT CCAGCATGGC 
CTCTCTCCCA TCAACTCTCA GACCACTCCT CGTGCCCCAT CCCCTGACAC ACATGAGGAA 
TACCCTATCA ATATTAAGCA GGTTTATAAG GCCTTCGCTG CTGTGCCACA CTCCCTGGCC 
GTACTGGAAC CCCCTCAGGA TCTTTATTCT CCACGGAACA ATGTCAGTGC AAAACAGCCA 
TCACCTGAGT TTCAGCCTGA GCTGAATAAT GAAGTGTTTA TGGATGAGTG TGATGGTGAG 
GGCAGTGGTC CTCGTCCCTC CCTCTTATCT GACCGCAGCG AGTACAGAAG CCTGGCAGCT 
GTGCCACGCC TGTCTCGCCC TTCAGTGGAG CCAAAGACCC CTTCGCCTCT GGGTAGGCAC 
AGTCCAGAAC AGCGCTCCTT CAAAGACAGA CAAAAGTATT TCGAGATCGA TGTGAAGCAA 
CAGACTCCAG ACAAACCCAA ACCTCGCATC TCACTGGTGG GAGAAGATGA CCTGAAGAAA 
ATGAAGGAGG AAGAAGCAAA GCGAATTGAA CAGAGGGCGC GAGAGTTCAT GTTGGATGAA 
GATGAGGAGG AGGAAGAAGA GGATCTTGCC AAGCAAGTGG CACATATGAA GGCTCACGGA 
AAGGTTGTTC TTGATGGTGT GGAGTACAAG GTAGAGAGCT TGGGTTCCCC GACTTCACGG 
CAGTGTGCCA CACCACCAAA CTATAGTGCC ACACCACCCA GCCACTGCGG AAGCTCAGGT 
CCTTCCTCTA TCGACGGTAA AGGAGACTCC CAGCGTAATT CAGTGGAAGA CAGTTTCAGA 
CTGGAGCAGA GACCCAATTC TATGACCGGA TTGATCCCAG TTTATCCCGG TGAATCTGCG 
GCTCCCATCC GGACGGCCAA AGCCGAGCGC AGGCATCAAG ATCGATTACG CATGCAGAGC 
CCAGAGCTCA CTGTCGCTTT GGACAAGGAG CTGTCGCCTG CTGAAAAACG AGCCCTGGAG 
GCAGAGAAGA GAGCCATGTG GAGAGCAGCC AGGATGAAGT CTTTAGAGCA GGACGCTTTG 
AAGGCTCAGA TGGTCATTGC CAAGTCTAAA GAGGGCAAGA AGCGAGGAAC CCTGGATCAG 
CTGTCAGAGT CCCCATCCCC TGCACCCACA CCCTCTCCAA CACCAATGGA AGATATCAGT 
CCACGGACAG TGACATCACC AGGCAGACTG TCCCTGTCAG AAAAGAAGTT TGACTACCGA 
CAATTTGCTG CCATTCCTTC TTCCAAACCC GTTTACGACA TTCAGTCTCC AGGTGCAGCT 
GATGACGTGC ACTTCATGGA TGAGTCCTCC AGTAACGCAG TGTCTGTTAT TGATCCAGAA 
GTTCCAGTGG CCGCCACGTC AGCTCTAGAA GAGATGGCTC TCTACAGTAA CAAACGTAAA 
CTACGCCAGG GCCGCCGCAG CCTGGAGGCT CCTGTGCCCA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301662.1
CDS	1129..6633
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKLTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_009303387.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303387.2

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGCTG ACCTTGAGCA TCCTGAGGCA GACTGGTGGA 
TTAGGCATCA GCATAGCAGG AGGGAAGGGA TCTACTCCCT ATAAAGGTGA CGATGAGGGT 
ATTTTCATCT CTAGGGTGTC AGAAGAAGGC CCTGCGGCTC GTGCTGGTGT CAAAGTTGGA 
GACAAACTTC TTGAGGTGAA TGGAGTAGAC CTGCACGGAG CGGAGCATCA TACAGCTGTT 
GAAGCTTTGC GCAATTCAGG AGCAGCCGTG GTCATGACTG TTTTGCGGGA GCGCATGGTG 
GAACCAGAAA ACGCCATCAC CACCACACCG TTGCGGCCAG AAGATGATTA TTTCCCCCGG 
GAGAGACGCT CCAGTGGGCT TCCTTTCCTC CTGGATCCTG ATTGTCCCGC TGTTAGCACT 
GGACCCGCAC AGCGACTAGC CACTTGTCTG ATCCGCAATG ATAAAGGCTT AGGCTTCAGC 
ATTGCTGGTG GGAAAGGGTC AACGCTGTAC AGAGTCGGAG ACACGGGCAT CTTCATCTCT 
CGTATTGCTG AAGGAGGAGC AGCTCACAGA GACAACATCC TGCAAGTGGG AGACAGGGTC 
ATATCAATCA ATGGTGTAGA CATGACAGAG GCCAGGCATG ACCAGGCAGT AGCGCTGCTT 
ACCGGCACCT CCCCCACCAT CACCCTGGTG GTGGATCGGG AACAAAGCAG TGTAGGCGGA 
GCCTCCCCTC GAACCCGACC ACATTCGCCC CCACCTCCAG AGCCCTCTGA CTCCCCTGAG 
CAAGAGGACG GTGGAGACGA GCACCTTGGA AACCATCTCA ACTGCCCCAT GGAAGATGAG 
TACCCTATTG AGGAAGTGAC GCTGATCAAA GCAGGTGGAC CCCTGGGTTT GAGCATTGTA 
GGGGGCAGCG ACCATGCCAG CCATCCATTT GGAATTAATG AACCTGGAGT GTTCATCTCA 
AAGGTCATCC CCAATGGCCT GGCCTCCCAG AGTGGACTTC GCGTGGGTGA TCGTATCTTG 
GAGGTGAACA GCATTGATCT GCGTCATGCC ACACACCAGG AGGCTGTTAG AGCCTTGCTT 
TCCAACAAAC AGGAGATCCG TATGCTTGTG CGCAGAGATC CTTCTCCCCC TGGCATGCAG 
GAAATTGTCA TTCACAAGCA ACCTGGAGAA AAGCTGGGAA TAAGCATTAG GGGCGGAGCT 
AAAGGTCATG CAGGAAACCC CTTTGACCCC ACAGATGAGG GCATCTTTAT TTCCAAGGTG 
AGCTCCAACG GTGCTGCTGC ACGAGATGGC CGACTGCGGG TGGGTATGCG TATTCTTGAG 
GTGGGCAACA ACAGTCTGCT GGGTATGACA CATACAGAAG CAGTGAGGGT CTTGCGTGCT 
TCTGGAGATT CCCTGGTCAT GCTTATTTGT GATGGCTTTG ACCCAAAAAG TGCTTCAACC 
ATAGAGGCAT CTCCTGGTGT TATTGCGAAT CCGTTTGCGG CAGGTATTGT TCGTAAAAAC 
AGTATGGAAA GCATCTCATC AGTCGACAGA GACCTGAGTC CAGAAGAGAT TGACATCATG 
CAGAAGGAGG TAGAAATGGT AAGAGAGACG TCCCAGTGGG AACGAGAGGA GATGGAAAAA 
GTGGAGCGCA TGCGCTTGGA GCGCGAGGAG GCAACTCGCT TGCTGGAGGA GGAAACTGAG 
AGTTTGAGCT CTGGACCACT GAAACTGGAC TATAAGACAC TGGCGGCTCT GCCTACAACT 
AGTCTACAGA AAGTTAATAG AGCTTCACCA TCTGAGCCTT TCAGGATTGA CAGTCCTGTC 
CGAGATGCTG CACACTCTCC ACACAACTCA CAGTCAAACA TTCATTTCCC TTCAAATGCA 
AACACCAAGG ATAATGCATC AACTAAACCT GGTGCCATCC AGCCTCTCTC TCGGGTGCGA 
CCCCCTGTGT CTCCAGCCAG TCAGGATGGC CACAGTAGTC CAAACCCCTT CCAGCATGGC 
CTCTCTCCCA TCAACTCTCA GACCACTCCT CGTGCCCCAT CCCCTGACAC ACATGAGGAA 
TACCCTATCA ATATTAAGCA GGTTTATAAG GCCTTCGCTG CTGTGCCACA CTCCCTGGCC 
GTACTGGAAC CCCCTCAGGA TCTTTATTCT CCACGGAACA ATGTCAGTGC AAAACAGCCA 
TCACCTGAGT TTCAGCCTGA GCTGAATAAT GAAGTGTTTA TGGATGAGTG TGATGGTGAG 
GGCAGTGGTC CTCGTCCCTC CCTCTTATCT GACCGCAGCG AGTACAGAAG CCTGGCAGCT 
GTGCCACGCC TGTCTCGCCC TTCAGTGGAG CCAAAGACCC CTTCGCCTCT GGGTAGGCAC 
AGTCCAGAAC AGCGCTCCTT CAAAGACAGA CAAAAGTATT TCGAGATCGA TGTGAAGCAA 
CAGACTCCAG ACAAACCCAA ACCTCGCATC TCACTGGTGG GAGAAGATGA CCTGAAGAAA 
ATGAAGGAGG AAGAAGCAAA GCGAATTGAA CAGAGGGCGC GAGAGTTCAT GTTGGATGAA 
GATGAGGAGG AGGAAGAAGA GGATCTTGCC AAGCAAGTGG CACATATGAA GGCTCACGGA 
AAGGTTGTTC TTGATGGTGT GGAGTACAAG GTAGAGAGCT TGGGTTCCCC GACTTCACGG 
CAGTGTGCCA CACCACCAAA CTATAGTGCC ACACCACCCA GCCACTGCGG AAGCTCAGGT 
CCTTCCTCTA TCGACGGTAA AGGAGACTCC CAGCGTAATT CAGTGGAAGA CAGTTTCAGA 
CTGGAGCAGA GACCCAATTC TATGACCGGA TTGATCCCAG TTTATCCCGG TGAATCTGCG 
GCTCCCATCC GGACGGCCAA AGCCGAGCGC AGGCATCAAG ATCGATTACG CATGCAGAGC 
CCAGAGCTCA CTGTCGCTTT GGACAAGGAG CTGTCGCCTG CTGAAAAACG AGCCCTGGAG 
GCAGAGAAGA GAGCCATGTG GAGAGCAGCC AGGATGAAGT CTTTAGAGCA GGACGCTTTG 
AAGGCTCAGA TGGTCATTGC CAAGTCTAAA GAGGGCAAGA AGCGAGGAAC CCTGGATCAG 
CTGTCAGAGT CCCCATCCCC TGCACCCACA CCCTCTCCAA CACCAATGGA AGATATCAGT 
CCACGGACAG TGACATCACC AGGCAGACTG TCCCTGTCAG AAAAGAAGTT TGACTACCGA 
CAATTTGCTG CCATTCCTTC TTCCAAACCC GTTTACGACA TTCAGTCTCC AGGTGCAGCT 
GATGACGTGC ACTTCATGGA TGAGTCCTCC AGTAACGCAG TGTCTGTTAT TGATCCAGAA 
GTTCCAGTGG CCGCCACGTC AGCTCTAGAA GAGATGGCTC TCTACAGTAA CAAACGTAAA 
CTACGCCAGG GCCGCCGCAG CCTGGAGGCT CCTGTGCCCA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_009303388.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301663.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X6, mRNA.

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CloneID	ODa40222
Clone ID Related Accession (Same CDS sequence)	XM_009303388.2
Accession Version	XM_009303388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein scribble homolog isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303388.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGTTTCAGCC TGAGCTGAAT AATGAAGTGT TTATGGATGA GTGTGATGGT 
GAGGGCAGTG GTCCTCGTCC CTCCCTCTTA TCTGACCGCA GCGAGTACAG AAGCCTGGCA 
GCTGTGCCAC GCCTGTCTCG CCCTTCAGTG GAGCCAAAGA CCCCTTCGCC TCTGGGTAGG 
CACAGTCCAG AACAGCGCTC CTTCAAAGAC AGACAAAAGT ATTTCGAGAT CGATGTGAAG 
CAACAGACTC CAGACAAACC CAAACCTCGC ATCTCACTGG TGGGAGAAGA TGACCTGAAG 
AAAATGAAGG AGGAAGAAGC AAAGCGAATT GAACAGAGGG CGCGAGAGTT CATGTTGGAT 
GAAGATGAGG AGGAGGAAGA AGAGGATCTT GCCAAGCAAG TGGCACATAT GAAGGCTCAC 
GGAAAGGTTG TTCTTGATGG TGTGGAGTAC AAGGTAGAGA GCTTGGGTTC CCCGACTTCA 
CGGCAGTGTG CCACACCACC AAACTATAGT GCCACACCAC CCAGCCACTG CGGAAGCTCA 
GGTCCTTCCT CTATCGACGG TAAAGGAGAC TCCCAGCGTA ATTCAGTGGA AGACAGTTTC 
AGACTGGAGC AGAGACCCAA TTCTATGACC GGATTGATCC CAGTTTATCC CGGTGAATCT 
GCGGCTCCCA TCCGGACGGC CAAAGCCGAG CGCAGGCATC AAGATCGATT ACGCATGCAG 
AGCCCAGAGC TCACTGTCGC TTTGGACAAG GAGCTGTCGC CTGCTGAAAA ACGAGCCCTG 
GAGGCAGAGA AGAGAGCCAT GTGGAGAGCA GCCAGGATGA AGTCTTTAGA GCAGGACGCT 
TTGAAGGCTC AGATGGTCAT TGCCAAGTCT AAAGAGGGCA AGAAGCGAGG AACCCTGGAT 
CAGCTGTCAG AGTCCCCATC CCCTGCACCC ACACCCTCTC CAACACCAAT GGAAGATATC 
AGTCCACGGA CAGTGACATC ACCAGGCAGA CTGTCTCCAG GTGCAGCTGA TGACGTGCAC 
TTCATGGATG AGTCCTCCAG TAACGCAGTG TCTGTTATTG ATCCAGAAGT TCCAGTGGCC 
GCCACGTCAG CTCTAGAAGA GATGGCTCTC TACAGTAACA AACGTAAACT ACGCCAGGGC 
CGCCGCAGCC TGGAGGCTCC TGTGCCCACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301663.1
CDS	1129..6621
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_009303388.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303388.2

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGTTTCAGCC TGAGCTGAAT AATGAAGTGT TTATGGATGA GTGTGATGGT 
GAGGGCAGTG GTCCTCGTCC CTCCCTCTTA TCTGACCGCA GCGAGTACAG AAGCCTGGCA 
GCTGTGCCAC GCCTGTCTCG CCCTTCAGTG GAGCCAAAGA CCCCTTCGCC TCTGGGTAGG 
CACAGTCCAG AACAGCGCTC CTTCAAAGAC AGACAAAAGT ATTTCGAGAT CGATGTGAAG 
CAACAGACTC CAGACAAACC CAAACCTCGC ATCTCACTGG TGGGAGAAGA TGACCTGAAG 
AAAATGAAGG AGGAAGAAGC AAAGCGAATT GAACAGAGGG CGCGAGAGTT CATGTTGGAT 
GAAGATGAGG AGGAGGAAGA AGAGGATCTT GCCAAGCAAG TGGCACATAT GAAGGCTCAC 
GGAAAGGTTG TTCTTGATGG TGTGGAGTAC AAGGTAGAGA GCTTGGGTTC CCCGACTTCA 
CGGCAGTGTG CCACACCACC AAACTATAGT GCCACACCAC CCAGCCACTG CGGAAGCTCA 
GGTCCTTCCT CTATCGACGG TAAAGGAGAC TCCCAGCGTA ATTCAGTGGA AGACAGTTTC 
AGACTGGAGC AGAGACCCAA TTCTATGACC GGATTGATCC CAGTTTATCC CGGTGAATCT 
GCGGCTCCCA TCCGGACGGC CAAAGCCGAG CGCAGGCATC AAGATCGATT ACGCATGCAG 
AGCCCAGAGC TCACTGTCGC TTTGGACAAG GAGCTGTCGC CTGCTGAAAA ACGAGCCCTG 
GAGGCAGAGA AGAGAGCCAT GTGGAGAGCA GCCAGGATGA AGTCTTTAGA GCAGGACGCT 
TTGAAGGCTC AGATGGTCAT TGCCAAGTCT AAAGAGGGCA AGAAGCGAGG AACCCTGGAT 
CAGCTGTCAG AGTCCCCATC CCCTGCACCC ACACCCTCTC CAACACCAAT GGAAGATATC 
AGTCCACGGA CAGTGACATC ACCAGGCAGA CTGTCTCCAG GTGCAGCTGA TGACGTGCAC 
TTCATGGATG AGTCCTCCAG TAACGCAGTG TCTGTTATTG ATCCAGAAGT TCCAGTGGCC 
GCCACGTCAG CTCTAGAAGA GATGGCTCTC TACAGTAACA AACGTAAACT ACGCCAGGGC 
CGCCGCAGCC TGGAGGCTCC TGTGCCCACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_009303389.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301664.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X7, mRNA.

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CloneID	ODa40223
Clone ID Related Accession (Same CDS sequence)	XM_009303389.2
Accession Version	XM_009303389.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein scribble homolog isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303389.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGTTTCAGCC TGAGCTGAAT AATGAAGTGT TTATGGATGA GTGTGATGGT 
GAGGGCAGTG GTCCTCGTCC CTCCCTCTTA TCTGACCGCA GCGAGTACAG AAGCCTGGCA 
GCTGTGCCAC GCCTGTCTCG CCCTTCAGTG GAGCCAAAGA CCCCTTCGCC TCTGGGTAGG 
CACAGTCCAG AACAGCGCTC CTTCAAAGAC AGACAAAAGT ATTTCGAGAT CGATGTGAAG 
CAACAGACTC CAGACAAACC CAAACCTCGC ATCTCACTGG TGGGAGAAGA TGACCTGAAG 
AAAATGAAGG AGGAAGAAGC AAAGCGAATT GAACAGAGGG CGCGAGAGTT CATGTTGGAT 
GAAGATGAGG AGGAGGAAGA AGAGGATCTT GCCAAGCAAG TGGCACATAT GAAGGCTCAC 
GGAAAGGTTG TTCTTGATGG TGTGGAGTAC AAGGTAGAGA GCTTGGGTTC CCCGACTTCA 
CGGCAGTGTG CCACACCACC AAACTATAGT GCCACACCAC CCAGCCACTG CGGAAGCTCA 
GGTCCTTCCT CTATCGACGG TAAAGGAGAC TCCCAGCGTA ATTCAGTGGA AGACAGTTTC 
AGACTGGAGC AGAGACCCAA TTCTATGACC GGATTGATCC CAGTTTATCC CGGTGAATCT 
GCGGCTCCCA TCCGGACGGC CAAAGCCGAG CGCAGGCATC AAGATCGATT ACGCATGCAG 
AGCCCAGAGC TCACTGTCGC TTTGGACAAG GAGCTGTCGC CTGCTGAAAA ACGAGCCCTG 
GAGGCAGAGA AGAGAGCCAT GTGGAGAGCA GCCAGGATGA AGTCTTTAGA GCAGGACGCT 
TTGAAGGCTC AGATGGTCAT TGCCAAGTCT AAAGAGGGCA AGAAGCGAGG AACCCTGGAT 
CAGCTGTCAG AGTCCCCATC CCCTGCACCC ACACCCTCTC CAACACCAAT GGAAGATATC 
AGTCCACGGA CAGTGACATC ACCAGGCAGA CTGTCCCTGT CAGAAAAGAA GTTTGACTAC 
CGACAATTTG CTGCCATTCC TTCTTCCAAA CCCGTTTACG ACATTCAGTC TCCAGGTGCA 
GCTGATGACG TGCACTTCAT GGATGAGTCC TCCAGTAACG CAGGCGACTT CCTTGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301664.1
CDS	1135..6594
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAGDFLG

Target ORF information:

RefSeq Version	XM_009303389.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303389.2

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGTTTCAGCC TGAGCTGAAT AATGAAGTGT TTATGGATGA GTGTGATGGT 
GAGGGCAGTG GTCCTCGTCC CTCCCTCTTA TCTGACCGCA GCGAGTACAG AAGCCTGGCA 
GCTGTGCCAC GCCTGTCTCG CCCTTCAGTG GAGCCAAAGA CCCCTTCGCC TCTGGGTAGG 
CACAGTCCAG AACAGCGCTC CTTCAAAGAC AGACAAAAGT ATTTCGAGAT CGATGTGAAG 
CAACAGACTC CAGACAAACC CAAACCTCGC ATCTCACTGG TGGGAGAAGA TGACCTGAAG 
AAAATGAAGG AGGAAGAAGC AAAGCGAATT GAACAGAGGG CGCGAGAGTT CATGTTGGAT 
GAAGATGAGG AGGAGGAAGA AGAGGATCTT GCCAAGCAAG TGGCACATAT GAAGGCTCAC 
GGAAAGGTTG TTCTTGATGG TGTGGAGTAC AAGGTAGAGA GCTTGGGTTC CCCGACTTCA 
CGGCAGTGTG CCACACCACC AAACTATAGT GCCACACCAC CCAGCCACTG CGGAAGCTCA 
GGTCCTTCCT CTATCGACGG TAAAGGAGAC TCCCAGCGTA ATTCAGTGGA AGACAGTTTC 
AGACTGGAGC AGAGACCCAA TTCTATGACC GGATTGATCC CAGTTTATCC CGGTGAATCT 
GCGGCTCCCA TCCGGACGGC CAAAGCCGAG CGCAGGCATC AAGATCGATT ACGCATGCAG 
AGCCCAGAGC TCACTGTCGC TTTGGACAAG GAGCTGTCGC CTGCTGAAAA ACGAGCCCTG 
GAGGCAGAGA AGAGAGCCAT GTGGAGAGCA GCCAGGATGA AGTCTTTAGA GCAGGACGCT 
TTGAAGGCTC AGATGGTCAT TGCCAAGTCT AAAGAGGGCA AGAAGCGAGG AACCCTGGAT 
CAGCTGTCAG AGTCCCCATC CCCTGCACCC ACACCCTCTC CAACACCAAT GGAAGATATC 
AGTCCACGGA CAGTGACATC ACCAGGCAGA CTGTCCCTGT CAGAAAAGAA GTTTGACTAC 
CGACAATTTG CTGCCATTCC TTCTTCCAAA CCCGTTTACG ACATTCAGTC TCCAGGTGCA 
GCTGATGACG TGCACTTCAT GGATGAGTCC TCCAGTAACG CAGGCGACTT CCTTGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_009303390.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301665.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X8, mRNA.

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CloneID	ODa40224
Clone ID Related Accession (Same CDS sequence)	XM_009303390.2
Accession Version	XM_009303390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein scribble homolog isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT GATCTTTATT CTCCACGGAA CAATGTCAGT 
GCAAAACAGC CATCACCTGA GTTTCAGCCT GAGCTGAATA ATGAAGTGTT TATGGATGAG 
TGTGATGGTG AGGGCAGTGG TCCTCGTCCC TCCCTCTTAT CTGACCGCAG CGAGTACAGA 
AGCCTGGCAG CTGTGCCACG CCTGTCTCGC CCTTCAGTGG AGCCAAAGAC CCCTTCGCCT 
CTGGGTAGGC ACAGTCCAGA ACAGCGCTCC TTCAAAGACA GACAAAAGTA TTTCGAGATC 
GATGTGAAGC AACAGACTCC AGACAAACCC AAACCTCGCA TCTCACTGGT GGGAGAAGAT 
GACCTGAAGA AAATGAAGGA GGAAGAAGCA AAGCGAATTG AACAGAGGGC GCGAGAGTTC 
ATGTTGGATG AAGATGAGGA GGAGGAAGAA GAGGATCTTG CCAAGCAAGT GGCACATATG 
AAGGCTCACG GAAAGGTTGT TCTTGATGGT GTGGAGTACA AGGTAGAGAG CTTGGGTTCC 
CCGACTTCAC GGCAGTGTGC CACACCACCA AACTATAGTG CCACACCACC CAGCCACTGC 
GGAAGCTCAG GTCCTTCCTC TATCGACGGT AAAGGAGACT CCCAGCGTAA TTCAGTGGAA 
GACAGTTTCA GACTGGAGCA GAGACCCAAT TCTATGACCG GATTGATCCC AGTTTATCCC 
GGTGAATCTG CGGCTCCCAT CCGGACGGCC AAAGCCGAGC GCAGGCATCA AGATCGATTA 
CGCATGCAGA GCCCAGAGCT CACTGTCGCT TTGGACAAGG AGCTGTCGCC TGCTGAAAAA 
CGAGCCCTGG AGGCAGAGAA GAGAGCCATG TGGAGAGCAG CCAGGATGAA GTCTTTAGAG 
CAGGACGCTT TGAAGGCTCA GATGGTCATT GCCAAGTCTA AAGAGGGCAA GAAGCGAGGA 
ACCCTGGATC AGCTGTCAGA GTCCCCATCC CCTGCACCCA CACCCTCTCC AACACCAATG 
GAAGATATCA GTCCACGGAC AGTGACATCA CCAGGCAGAC TGTCCCTGTC AGAAAAGAAG 
TTTGACTACC GACAATTTGC TGCCATTCCT TCTTCCAAAC CCGTTTACGA CATTCAGTCT 
CCAGGTGCAG CTGATGACGT GCACTTCATG GATGAGTCCT CCAGTAACGC AGTGTCTGTT 
ATTGATCCAG AAGTTCCAGT GGCCGCCACG TCAGCTCTAG AAGAGATGGC TCTCTACAGT 
AACAAACGTA AACTACGCCA GGGCCGCCGC AGCCTGGAGG CTCCTGTGCC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301665.1
CDS	1126..6582
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_009303390.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303390.2

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT GATCTTTATT CTCCACGGAA CAATGTCAGT 
GCAAAACAGC CATCACCTGA GTTTCAGCCT GAGCTGAATA ATGAAGTGTT TATGGATGAG 
TGTGATGGTG AGGGCAGTGG TCCTCGTCCC TCCCTCTTAT CTGACCGCAG CGAGTACAGA 
AGCCTGGCAG CTGTGCCACG CCTGTCTCGC CCTTCAGTGG AGCCAAAGAC CCCTTCGCCT 
CTGGGTAGGC ACAGTCCAGA ACAGCGCTCC TTCAAAGACA GACAAAAGTA TTTCGAGATC 
GATGTGAAGC AACAGACTCC AGACAAACCC AAACCTCGCA TCTCACTGGT GGGAGAAGAT 
GACCTGAAGA AAATGAAGGA GGAAGAAGCA AAGCGAATTG AACAGAGGGC GCGAGAGTTC 
ATGTTGGATG AAGATGAGGA GGAGGAAGAA GAGGATCTTG CCAAGCAAGT GGCACATATG 
AAGGCTCACG GAAAGGTTGT TCTTGATGGT GTGGAGTACA AGGTAGAGAG CTTGGGTTCC 
CCGACTTCAC GGCAGTGTGC CACACCACCA AACTATAGTG CCACACCACC CAGCCACTGC 
GGAAGCTCAG GTCCTTCCTC TATCGACGGT AAAGGAGACT CCCAGCGTAA TTCAGTGGAA 
GACAGTTTCA GACTGGAGCA GAGACCCAAT TCTATGACCG GATTGATCCC AGTTTATCCC 
GGTGAATCTG CGGCTCCCAT CCGGACGGCC AAAGCCGAGC GCAGGCATCA AGATCGATTA 
CGCATGCAGA GCCCAGAGCT CACTGTCGCT TTGGACAAGG AGCTGTCGCC TGCTGAAAAA 
CGAGCCCTGG AGGCAGAGAA GAGAGCCATG TGGAGAGCAG CCAGGATGAA GTCTTTAGAG 
CAGGACGCTT TGAAGGCTCA GATGGTCATT GCCAAGTCTA AAGAGGGCAA GAAGCGAGGA 
ACCCTGGATC AGCTGTCAGA GTCCCCATCC CCTGCACCCA CACCCTCTCC AACACCAATG 
GAAGATATCA GTCCACGGAC AGTGACATCA CCAGGCAGAC TGTCCCTGTC AGAAAAGAAG 
TTTGACTACC GACAATTTGC TGCCATTCCT TCTTCCAAAC CCGTTTACGA CATTCAGTCT 
CCAGGTGCAG CTGATGACGT GCACTTCATG GATGAGTCCT CCAGTAACGC AGTGTCTGTT 
ATTGATCCAG AAGTTCCAGT GGCCGCCACG TCAGCTCTAG AAGAGATGGC TCTCTACAGT 
AACAAACGTA AACTACGCCA GGGCCGCCGC AGCCTGGAGG CTCCTGTGCC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_009303392.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301667.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X10, mRNA.

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CloneID	ODa40225
Clone ID Related Accession (Same CDS sequence)	XM_009303392.2
Accession Version	XM_009303392.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein scribble homolog isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGTTTCAGCC TGAGCTGAAT AATGAAGTGT TTATGGATGA GTGTGATGGT 
GAGGGCAGTG GTCCTCGTCC CTCCCTCTTA TCTGACCGCA GCGAGTACAG AAGCCTGGCA 
GCTGTGCCAC GCCTGTCTCG CCCTTCAGTG GAGCCAAAGA CCCCTTCGCC TCTGGGTAGG 
CACAGTCCAG AACAGCGCTC CTTCAAAGAC AGACAAAAGT ATTTCGAGAT CGATGTGAAG 
CAACAGACTC CAGACAAACC CAAACCTCGC ATCTCACTGG TGGGAGAAGA TGACCTGAAG 
AAAATGAAGG AGGAAGAAGC AAAGCGAATT GAACAGAGGG CGCGAGAGTT CATGTTGGAT 
GAAGATGAGG AGGAGGAAGA AGAGGATCTT GCCAAGCAAG TGGCACATAT GAAGGCTCAC 
GGAAAGGTTG TTCTTGATGG TGTGGAGTAC AAGGTAGAGA GCTTGGGTTC CCCGACTTCA 
CGGCAGTGTG CCACACCACC AAACTATAGT GCCACACCAC CCAGCCACTG CGGAAGCTCA 
GGTCCTTCCT CTATCGACGG TAAAGGAGAC TCCCAGCGTA ATTCAGTGGA AGACAGTTTC 
AGACTGGAGC AGAGACCCAA TTCTATGACC GGATTGATCC CAGTTTATCC CGGTGAATCT 
GCGGCTCCCA TCCGGACGGC CAAAGCCGAG CGCAGGCATC AAGATCGATT ACGCATGCAG 
AGCCCAGAGC TCACTGTCGC TTTGGACAAG GAGCTGTCGC CTGCTGAAAA ACGAGCCCTG 
GAGGCAGAGA AGAGAGCCAT GTGGAGAGCA GCCAGGATGA AGTCTTTAGA GCAGGACGCT 
TTGAAGGCTC AGATGGTCAT TGCCAAGTCT AAAGAGGGCA AGAAGCGAGG AACCCTGGAT 
CAGCTGTCAG AGTCCCCATC CCCTGCACCC ACACCCTCTC CAACACCAAT GGAAGATATC 
AGTCCACGGA CAGTGACATC ACCAGGCAGA CTGTCTCCAG GTGCAGCTGA TGACGTGCAC 
TTCATGGATG AGTCCTCCAG TAACGCAGGC GACTTCCTTG GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301667.1
CDS	1133..6517
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSPGAADDVHFMDESSSNAGDFLG

Target ORF information:

RefSeq Version	XM_009303392.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303392.2

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGTTTCAGCC TGAGCTGAAT AATGAAGTGT TTATGGATGA GTGTGATGGT 
GAGGGCAGTG GTCCTCGTCC CTCCCTCTTA TCTGACCGCA GCGAGTACAG AAGCCTGGCA 
GCTGTGCCAC GCCTGTCTCG CCCTTCAGTG GAGCCAAAGA CCCCTTCGCC TCTGGGTAGG 
CACAGTCCAG AACAGCGCTC CTTCAAAGAC AGACAAAAGT ATTTCGAGAT CGATGTGAAG 
CAACAGACTC CAGACAAACC CAAACCTCGC ATCTCACTGG TGGGAGAAGA TGACCTGAAG 
AAAATGAAGG AGGAAGAAGC AAAGCGAATT GAACAGAGGG CGCGAGAGTT CATGTTGGAT 
GAAGATGAGG AGGAGGAAGA AGAGGATCTT GCCAAGCAAG TGGCACATAT GAAGGCTCAC 
GGAAAGGTTG TTCTTGATGG TGTGGAGTAC AAGGTAGAGA GCTTGGGTTC CCCGACTTCA 
CGGCAGTGTG CCACACCACC AAACTATAGT GCCACACCAC CCAGCCACTG CGGAAGCTCA 
GGTCCTTCCT CTATCGACGG TAAAGGAGAC TCCCAGCGTA ATTCAGTGGA AGACAGTTTC 
AGACTGGAGC AGAGACCCAA TTCTATGACC GGATTGATCC CAGTTTATCC CGGTGAATCT 
GCGGCTCCCA TCCGGACGGC CAAAGCCGAG CGCAGGCATC AAGATCGATT ACGCATGCAG 
AGCCCAGAGC TCACTGTCGC TTTGGACAAG GAGCTGTCGC CTGCTGAAAA ACGAGCCCTG 
GAGGCAGAGA AGAGAGCCAT GTGGAGAGCA GCCAGGATGA AGTCTTTAGA GCAGGACGCT 
TTGAAGGCTC AGATGGTCAT TGCCAAGTCT AAAGAGGGCA AGAAGCGAGG AACCCTGGAT 
CAGCTGTCAG AGTCCCCATC CCCTGCACCC ACACCCTCTC CAACACCAAT GGAAGATATC 
AGTCCACGGA CAGTGACATC ACCAGGCAGA CTGTCTCCAG GTGCAGCTGA TGACGTGCAC 
TTCATGGATG AGTCCTCCAG TAACGCAGGC GACTTCCTTG GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_009303393.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301668.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X11, mRNA.

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CloneID	ODa40226
Clone ID Related Accession (Same CDS sequence)	XM_009303393.2
Accession Version	XM_009303393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein scribble homolog isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGTTTCAGCC TGAGCTGAAT AATGAAGTGT TTATGGATGA GTGTGATGGT 
GAGGGCAGTG GTCCTCGTCC CTCCCTCTTA TCTGACCGCA GCGAGTACAG AAGCCTGGCA 
GCTGTGCCAC GCCTGTCTCG CCCTTCAGTG GAGCCAAAGA CCCCTTCGCC TCTGGGTAGG 
CACAGTCCAG AACAGCGCTC CTTCAAAGAC AGACAAAAGT ATTTCGAGAT CGATGTGAAG 
CAACAGACTC CAGACAAACC CAAACCTCGC ATCTCACTGG TGGGAGAAGA TGACCTGAAG 
AAAATGAAGG AGGAAGAAGC AAAGCGAATT GAACAGAGGG CGCGAGAGTT CATGTTGGAT 
GAAGATGAGG AGGAGGAAGA AGAGGATCTT GCCAAGCAAG TGGCACATAT GAAGGCTCAC 
GGAAAGGTTG TTCTTGATGG TGTGGAGTAC AAGGTAGAGA GCTTGGGTTC CCCGACTTCA 
CGGCAGTGTG CCACACCACC AAACTATAGT GCCACACCAC CCAGCCACTG CGGAAGCTCA 
GGTCCTTCCT CTATCGACGG TAAAGGAGAC TCCCAGCGTA ATTCAGTGGA AGACAGTTTC 
AGACTGGAGC AGAGACCCAA TTCTATGACC GGATTGATCC CAGTTTATCC CGGTGAATCT 
GCGGCTCCCA TCCGGACGGC CAAAGCCGAG CGCAGGCATC AAGATCGATT ACGCATGCAG 
AGCCCAGAGC TCACTGTCGC TTTGGACAAG GAGCTGTCGC CTGCTGAAAA ACGAGCCCTG 
GAGGCAGAGA AGAGAGCCAT GTGGAGAGCA GCCAGGCCTT ATGGCTTAGA GGAGGACGTA 
AGGCAGTATG AGCAAGACCT GGCTAAGCGG CTCTACCAGT CCCGTGTGAG GGCATCACTG 
GGCAGCGCTG CAGCCCCTGC CTCTTCCTCC TGCTCCTCTT CCTCTTCCTG TCAGCCCAGG 
TCTGCCCTTC ACAGCCAGAA AGCATCATGG GGGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301668.1
CDS	1292..6610
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRSALHSQKASWGL

Target ORF information:

RefSeq Version	XM_009303393.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303393.2

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT CCTCGTGCCC CATCCCCTGA CACACATGAG 
GAATACCCTA TCAATATTAA GCAGGTTTAT AAGGCCTTCG CTGCTGTGCC ACACTCCCTG 
GCCGTACTGG AACCCCCTCA GGATCTTTAT TCTCCACGGA ACAATGTCAG TGCAAAACAG 
CCATCACCTG AGTTTCAGCC TGAGCTGAAT AATGAAGTGT TTATGGATGA GTGTGATGGT 
GAGGGCAGTG GTCCTCGTCC CTCCCTCTTA TCTGACCGCA GCGAGTACAG AAGCCTGGCA 
GCTGTGCCAC GCCTGTCTCG CCCTTCAGTG GAGCCAAAGA CCCCTTCGCC TCTGGGTAGG 
CACAGTCCAG AACAGCGCTC CTTCAAAGAC AGACAAAAGT ATTTCGAGAT CGATGTGAAG 
CAACAGACTC CAGACAAACC CAAACCTCGC ATCTCACTGG TGGGAGAAGA TGACCTGAAG 
AAAATGAAGG AGGAAGAAGC AAAGCGAATT GAACAGAGGG CGCGAGAGTT CATGTTGGAT 
GAAGATGAGG AGGAGGAAGA AGAGGATCTT GCCAAGCAAG TGGCACATAT GAAGGCTCAC 
GGAAAGGTTG TTCTTGATGG TGTGGAGTAC AAGGTAGAGA GCTTGGGTTC CCCGACTTCA 
CGGCAGTGTG CCACACCACC AAACTATAGT GCCACACCAC CCAGCCACTG CGGAAGCTCA 
GGTCCTTCCT CTATCGACGG TAAAGGAGAC TCCCAGCGTA ATTCAGTGGA AGACAGTTTC 
AGACTGGAGC AGAGACCCAA TTCTATGACC GGATTGATCC CAGTTTATCC CGGTGAATCT 
GCGGCTCCCA TCCGGACGGC CAAAGCCGAG CGCAGGCATC AAGATCGATT ACGCATGCAG 
AGCCCAGAGC TCACTGTCGC TTTGGACAAG GAGCTGTCGC CTGCTGAAAA ACGAGCCCTG 
GAGGCAGAGA AGAGAGCCAT GTGGAGAGCA GCCAGGCCTT ATGGCTTAGA GGAGGACGTA 
AGGCAGTATG AGCAAGACCT GGCTAAGCGG CTCTACCAGT CCCGTGTGAG GGCATCACTG 
GGCAGCGCTG CAGCCCCTGC CTCTTCCTCC TGCTCCTCTT CCTCTTCCTG TCAGCCCAGG 
TCTGCCCTTC ACAGCCAGAA AGCATCATGG GGGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_009303394.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301669.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X12, mRNA.

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CloneID	ODa40227
Clone ID Related Accession (Same CDS sequence)	XM_009303394.2
Accession Version	XM_009303394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein scribble homolog isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT GATCTTTATT CTCCACGGAA CAATGTCAGT 
GCAAAACAGC CATCACCTGA GACCCCTTCG CCTCTGGGTA GGCACAGTCC AGAACAGCGC 
TCCTTCAAAG ACAGACAAAA GTATTTCGAG ATCGATGTGA AGCAACAGAC TCCAGACAAA 
CCCAAACCTC GCATCTCACT GGTGGGAGAA GATGACCTGA AGAAAATGAA GGAGGAAGAA 
GCAAAGCGAA TTGAACAGAG GGCGCGAGAG TTCATGTTGG ATGAAGATGA GGAGGAGGAA 
GAAGAGGATC TTGCCAAGCA AGTGGCACAT ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT 
GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT TCCCCGACTT CACGGCAGTG TGCCACACCA 
CCAAACTATA GTGCCACACC ACCCAGCCAC TGCGGAAGCT CAGGTCCTTC CTCTATCGAC 
GGTAAAGGAG ACTCCCAGCG TAATTCAGTG GAAGACAGTT TCAGACTGGA GCAGAGACCC 
AATTCTATGA CCGGATTGAT CCCAGTTTAT CCCGGTGAAT CTGCGGCTCC CATCCGGACG 
GCCAAAGCCG AGCGCAGGCA TCAAGATCGA TTACGCATGC AGAGCCCAGA GCTCACTGTC 
GCTTTGGACA AGGAGCTGTC GCCTGCTGAA AAACGAGCCC TGGAGGCAGA GAAGAGAGCC 
ATGTGGAGAG CAGCCAGGAT GAAGTCTTTA GAGCAGGACG CTTTGAAGGC TCAGATGGTC 
ATTGCCAAGT CTAAAGAGGG CAAGAAGCGA GGAACCCTGG ATCAGCTGTC AGAGTCCCCA 
TCCCCTGCAC CCACACCCTC TCCAACACCA ATGGAAGATA TCAGTCCACG GACAGTGACA 
TCACCAGGCA GACTGTCCCT GTCAGAAAAG AAGTTTGACT ACCGACAATT TGCTGCCATT 
CCTTCTTCCA AACCCGTTTA CGACATTCAG TCTCCAGGTG CAGCTGATGA CGTGCACTTC 
ATGGATGAGT CCTCCAGTAA CGCAGTGTCT GTTATTGATC CAGAAGTTCC AGTGGCCGCC 
ACGTCAGCTC TAGAAGAGAT GGCTCTCTAC AGTAACAAAC GTAAACTACG CCAGGGCCGC 
CGCAGCCTGG AGGCTCCTGT GCCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301669.1
CDS	1120..6429
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTDLYSPRNNVSAKQPSPETPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_009303394.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303394.2

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGAGCTTCA CCATCTGAGC CTTTCAGGAT TGACAGTCCT 
GTCCGAGATG CTGCACACTC TCCACACAAC TCACAGTCAA ACATTCATTT CCCTTCAAAT 
GCAAACACCA AGGATAATGC ATCAACTAAA CCTGGTGCCA TCCAGCCTCT CTCTCGGGTG 
CGACCCCCTG TGTCTCCAGC CAGTCAGGAT GGCCACAGTA GTCCAAACCC CTTCCAGCAT 
GGCCTCTCTC CCATCAACTC TCAGACCACT GATCTTTATT CTCCACGGAA CAATGTCAGT 
GCAAAACAGC CATCACCTGA GACCCCTTCG CCTCTGGGTA GGCACAGTCC AGAACAGCGC 
TCCTTCAAAG ACAGACAAAA GTATTTCGAG ATCGATGTGA AGCAACAGAC TCCAGACAAA 
CCCAAACCTC GCATCTCACT GGTGGGAGAA GATGACCTGA AGAAAATGAA GGAGGAAGAA 
GCAAAGCGAA TTGAACAGAG GGCGCGAGAG TTCATGTTGG ATGAAGATGA GGAGGAGGAA 
GAAGAGGATC TTGCCAAGCA AGTGGCACAT ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT 
GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT TCCCCGACTT CACGGCAGTG TGCCACACCA 
CCAAACTATA GTGCCACACC ACCCAGCCAC TGCGGAAGCT CAGGTCCTTC CTCTATCGAC 
GGTAAAGGAG ACTCCCAGCG TAATTCAGTG GAAGACAGTT TCAGACTGGA GCAGAGACCC 
AATTCTATGA CCGGATTGAT CCCAGTTTAT CCCGGTGAAT CTGCGGCTCC CATCCGGACG 
GCCAAAGCCG AGCGCAGGCA TCAAGATCGA TTACGCATGC AGAGCCCAGA GCTCACTGTC 
GCTTTGGACA AGGAGCTGTC GCCTGCTGAA AAACGAGCCC TGGAGGCAGA GAAGAGAGCC 
ATGTGGAGAG CAGCCAGGAT GAAGTCTTTA GAGCAGGACG CTTTGAAGGC TCAGATGGTC 
ATTGCCAAGT CTAAAGAGGG CAAGAAGCGA GGAACCCTGG ATCAGCTGTC AGAGTCCCCA 
TCCCCTGCAC CCACACCCTC TCCAACACCA ATGGAAGATA TCAGTCCACG GACAGTGACA 
TCACCAGGCA GACTGTCCCT GTCAGAAAAG AAGTTTGACT ACCGACAATT TGCTGCCATT 
CCTTCTTCCA AACCCGTTTA CGACATTCAG TCTCCAGGTG CAGCTGATGA CGTGCACTTC 
ATGGATGAGT CCTCCAGTAA CGCAGTGTCT GTTATTGATC CAGAAGTTCC AGTGGCCGCC 
ACGTCAGCTC TAGAAGAGAT GGCTCTCTAC AGTAACAAAC GTAAACTACG CCAGGGCCGC 
CGCAGCCTGG AGGCTCCTGT GCCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477414.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333089.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X1, mRNA.

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CloneID	ODa143208
Clone ID Related Accession (Same CDS sequence)	XM_021477414.1
Accession Version	XM_021477414.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGCC TTATGGCTTA 
GAGGAGGACG TAAGGCAGTA TGAGCAAGAC CTGGCTAAGC GGCTCTACCA GTCCCGTGTG 
AGGGCATCAC TGGGCAGCGC TGCAGCCCCT GCCTCTTCCT CCTGCTCCTC TTCCTCTTCC 
TGTCAGCCCA GGATGAAGTC TTTAGAGCAG GACGCTTTGA AGGCTCAGAT GGTCATTGCC 
AAGTCTAAAG AGGGCAAGAA GCGAGGAACC CTGGATCAGC TGTCAGAGTC CCCATCCCCT 
GCACCCACAC CCTCTCCAAC ACCAATGGAA GATATCAGTC CACGGACAGT GACATCACCA 
GGCAGACTGT CCCTGTCAGA AAAGAAGTTT GACTACCGAC AATTTGCTGC CATTCCTTCT 
TCCAAACCCG TTTACGACAT TCAGTCTCCA GGTGCAGCTG ATGACGTGCA CTTCATGGAT 
GAGTCCTCCA GTAACGCAGT GTCTGTTATT GATCCAGAAG TTCCAGTGGC CGCCACGTCA 
GCTCTAGAAG AGATGGCTCT CTACAGTAAC AAACGTAAAC TACGCCAGGG CCGCCGCAGC 
CTGGAGGCTC CTGTGCCCAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333089.1
CDS	1071..7214
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477414.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477414.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGCC TTATGGCTTA 
GAGGAGGACG TAAGGCAGTA TGAGCAAGAC CTGGCTAAGC GGCTCTACCA GTCCCGTGTG 
AGGGCATCAC TGGGCAGCGC TGCAGCCCCT GCCTCTTCCT CCTGCTCCTC TTCCTCTTCC 
TGTCAGCCCA GGATGAAGTC TTTAGAGCAG GACGCTTTGA AGGCTCAGAT GGTCATTGCC 
AAGTCTAAAG AGGGCAAGAA GCGAGGAACC CTGGATCAGC TGTCAGAGTC CCCATCCCCT 
GCACCCACAC CCTCTCCAAC ACCAATGGAA GATATCAGTC CACGGACAGT GACATCACCA 
GGCAGACTGT CCCTGTCAGA AAAGAAGTTT GACTACCGAC AATTTGCTGC CATTCCTTCT 
TCCAAACCCG TTTACGACAT TCAGTCTCCA GGTGCAGCTG ATGACGTGCA CTTCATGGAT 
GAGTCCTCCA GTAACGCAGT GTCTGTTATT GATCCAGAAG TTCCAGTGGC CGCCACGTCA 
GCTCTAGAAG AGATGGCTCT CTACAGTAAC AAACGTAAAC TACGCCAGGG CCGCCGCAGC 
CTGGAGGCTC CTGTGCCCAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477415.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333090.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X2, mRNA.

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CloneID	ODa143209
Clone ID Related Accession (Same CDS sequence)	XM_021477415.1
Accession Version	XM_021477415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGGGTCTT CAGAGGAGAG CCACACCACA CCCTAGTGAG 
TTAAAGGTGA TGAAGAATGT AATTGAAGCA AGAAGAAATG AGGCTTATAC AGCCAGACCA 
GATGAAGATC TAGAATCCCC AGATGCTGAG GAGAAACGGT TAAGTGGTCT GTCCAATCAG 
AGTCATGATT CTCAGGCATC CAGTAGCACC ACCTCAGCCA CTTCCCATGA GGACAAGCAG 
GGTATAAACA GTACAGGGGT GGAGCTTAAT GATGGACAGG TTCAAGAAGA GGAGGATCTG 
GATGAGATGG AGGTGGAGTA TACTGAGCCT ACTGTGCACT TTGCTGAAGA GCCCATCATC 
CGTGGTGGTG ATGAGGACGA CGATTATGAT AATGACGATG ATGACGCTGA AAGGAGTGAC 
GAGGTGTCTC AAACGCCATC TTTTCCTGCC GAGAAACAGC GGCTCATACG TAAAGACACA 
CCACACTACA AAAAGCACTT CAAGATCACC AAGCTGCCCA AACCAGAGAC AGTCGCCGCC 
CTGCTGCAAG GCTTCAGTCA TGATGGCCTG TCACCTACTG CTCACACCCC TGAGAACGAG 
AGGGATGGAG AGGATGACGA GGAAGAGGAG GAGGATGAAG ACGAGGAGGA CGATCTCCAT 
ACTCCTTTCC AGCACAGACT AGATGCAGCA GAGCTAGAAG ACATCAGAAT GAGTCAGATG 
AACTCGTCCC TGCAGCCAGT GAAGGGGGTG TCATTTGATC AAGTCAATAA TCTGCTGATT 
GAACCTGCTC GAATTGAGGA GGAAGAGCTG ACCTTGAGCA TCCTGAGGCA GACTGGTGGA 
TTAGGCATCA GCATAGCAGG AGGGAAGGGA TCTACTCCCT ATAAAGGTGA CGATGAGGGT 
ATTTTCATCT CTAGGGTGTC AGAAGAAGGC CCTGCGGCTC GTGCTGGTGT CAAAGTTGGA 
GACAAACTTC TTGAGGTGAA TGGAGTAGAC CTGCACGGAG CGGAGCATCA TACAGCTGTT 
GAAGCTTTGC GCAATTCAGG AGCAGCCGTG GTCATGACTG TTTTGCGGGA GCGCATGGTG 
GAACCAGAAA ACGCCATCAC CACCACACCG TTGCGGCCAG AAGATGATTA TTTCCCCCGG 
GAGAGACGCT CCAGTGGGCT TCCTTTCCTC CTGGATCCTG ATTGTCCCGC TGTTAGCACT 
GGACCCGCAC AGCGACTAGC CACTTGTCTG ATCCGCAATG ATAAAGGCTT AGGCTTCAGC 
ATTGCTGGTG GGAAAGGGTC AACGCTGTAC AGAGTCGGAG ACACGGGCAT CTTCATCTCT 
CGTATTGCTG AAGGAGGAGC AGCTCACAGA GACAACATCC TGCAAGTGGG AGACAGGGTC 
ATATCAATCA ATGGTGTAGA CATGACAGAG GCCAGGCATG ACCAGGCAGT AGCGCTGCTT 
ACCGGCACCT CCCCCACCAT CACCCTGGTG GTGGATCGGG AACAAAGCAG TGTAGGCGGA 
GCCTCCCCTC GAACCCGACC ACATTCGCCC CCACCTCCAG AGCCCTCTGA CTCCCCTGAG 
CAAGAGGACG GTGGAGACGA GCACCTTGGA AACCATCTCA ACTGCCCCAT GGAAGATGAG 
TACCCTATTG AGGAAGTGAC GCTGATCAAA GCAGGTGGAC CCCTGGGTTT GAGCATTGTA 
GGGGGCAGCG ACCATGCCAG CCATCCATTT GGAATTAATG AACCTGGAGT GTTCATCTCA 
AAGGTCATCC CCAATGGCCT GGCCTCCCAG AGTGGACTTC GCGTGGGTGA TCGTATCTTG 
GAGGTGAACA GCATTGATCT GCGTCATGCC ACACACCAGG AGGCTGTTAG AGCCTTGCTT 
TCCAACAAAC AGGAGATCCG TATGCTTGTG CGCAGAGATC CTTCTCCCCC TGGCATGCAG 
GAAATTGTCA TTCACAAGCA ACCTGGAGAA AAGCTGGGAA TAAGCATTAG GGGCGGAGCT 
AAAGGTCATG CAGGAAACCC CTTTGACCCC ACAGATGAGG GCATCTTTAT TTCCAAGGTG 
AGCTCCAACG GTGCTGCTGC ACGAGATGGC CGACTGCGGG TGGGTATGCG TATTCTTGAG 
GTGGGCAACA ACAGTCTGCT GGGTATGACA CATACAGAAG CAGTGAGGGT CTTGCGTGCT 
TCTGGAGATT CCCTGGTCAT GCTTATTTGT GATGGCTTTG ACCCAAAAAG TGCTTCAACC 
ATAGAGGCAT CTCCTGGTGT TATTGCGAAT CCGTTTGCGG CAGGTATTGT TCGTAAAAAC 
AGTATGGAAA GCATCTCATC AGTCGACAGA GACCTGAGTC CAGAAGAGAT TGACATCATG 
CAGAAGGAGG TAGAAATGGT AAGAGAGACG TCCCAGTGGG AACGAGAGGA GATGGAAAAA 
GTGGAGCGCA TGCGCTTGGA GCGCGAGGAG GCAACTCGCT TGCTGGAGGA GGAAACTGAG 
AGTTTGAGCT CTGGACCACT GAAACTGGAC TATAAGACAC TGGCGGCTCT GCCTACAACT 
AGTCTACAGA AAGTTAATAG ATTTTCTCCC TCCTTTTCCA CATTTGCCAA AATGGAGGCT 
GCCATGCCGG CTGGCCCCGT AGACCCCCTG GGTTTTGAGA AAGTCCATGC TTCTATGTAC 
CATGTAGAAC CTGTTGCCCT CCCTTCATCT CCAATCTGCC CAGCCGCAAG TTCAGCGGCT 
TCAAAACAGC CCCAAGCTGA CTCACGGGAC CTGGTCTTAG ATCTAGGAAT GGCTCAAGAG 
GAGGAGGAAT GTCTTGTGGA CTCCCAACCA ATCTGCTTTA GTGAAAACCC ATTTCTTGTG 
GCTAACCGCA AGGGCAAAGG CTGCCCACCA GAAGAGCGCA TTTTATCAGG GCCACCTGTG 
GGTTACGGCA AGCATGGGCA GCTGCAGCCC TGGTTGTACA GCAAGGCTTC ACCATCTGAG 
CCTTTCAGGA TTGACAGTCC TGTCCGAGAT GCTGCACACT CTCCACACAA CTCACAGCAG 
TCTCACAAAT CTTCCTCTGC GATGCTTGGA AGAGAGGTTC GATATTCAAA CATTCATTTC 
CCTTCAAATG CAAACACCAA GGATAATGCA TCAACTAAAC CTGGTGCCAT CCAGCCTCTC 
TCTCGGGTGC GACCCCCTGT GTCTCCAGCC AGTCAGGATG GCCACAGTAG TCCAAACCCC 
TTCCAGCATG GCCTCTCTCC CATCAACTCT CAGACCACTC CTCGTGCCCC ATCCCCTGAC 
ACACATGAGG AATACCCTAT CAATATTAAG CAGGTTTATA AGGCCTTCGC TGCTGTGCCA 
CACTCCCTGG CCGTACTGGA ACCCCCTCAG GATCTTTATT CTCCACGGAA CAATGTCAGT 
GCAAAACAGC CATCACCTGA GTTTCAGCCT GAGCTGAATA ATGAAGTGTT TATGGATGAG 
TGTGATGGTG AGGGCAGTGG TCCTCGTCCC TCCCTCTTAT CTGACCGCAG CGAGTACAGA 
AGCCTGGCAG CTGTGCCACG CCTGTCTCGC CCTTCAGTGG AGCCAAAGAC CCCTTCGCCT 
CTGGGTAGGC ACAGTCCAGA ACAGCGCTCC TTCAAAGACA GACAAAAGTA TTTCGAGATC 
GATGTGAAGC AACAGACTCC AGACAAACCC AAACCTCGCA TCTCACTGGT GGGAGAAGAT 
GACCTGAAGA AAATGAAGGA GGAAGAAGCA AAGCGAATTG AACAGAGGGC GCGAGAGTTC 
ATGTTGGATG AAGATGAGGA GGAGGAAGAA GAGGATCTTG CCAAGCAAGT GGCACATATG 
AAGGCTCACG GAAAGGTTGT TCTTGATGGT GTGGAGTACA AGGTAGAGAG CTTGGGTTCC 
CCGACTTCAC GGCAGTGTGC CACACCACCA AACTATAGTG CCACACCACC CAGCCACTGC 
GGAAGCTCAG GTCCTTCCTC TATCGACGGT AAAGGAGACT CCCAGCGTAA TTCAGTGGAA 
GACAGTTTCA GACTGGAGCA GAGACCCAAT TCTATGACCG GATTGATCCC AGTTTATCCC 
GGTGAATCTG CGGCTCCCAT CCGGACGGCC AAAGCCGAGC GCAGGCATCA AGATCGATTA 
CGCATGCAGA GCCCAGAGCT CACTGTCGCT TTGGACAAGG AGCTGTCGCC TGCTGAAAAA 
CGAGCCCTGG AGGCAGAGAA GAGAGCCATG TGGAGAGCAG CCAGGCCTTA TGGCTTAGAG 
GAGGACGTAA GGCAGTATGA GCAAGACCTG GCTAAGCGGC TCTACCAGTC CCGTGTGAGG 
GCATCACTGG GCAGCGCTGC AGCCCCTGCC TCTTCCTCCT GCTCCTCTTC CTCTTCCTGT 
CAGCCCAGGA TGAAGTCTTT AGAGCAGGAC GCTTTGAAGG CTCAGATGGT CATTGCCAAG 
TCTAAAGAGG GCAAGAAGCG AGGAACCCTG GATCAGCTGT CAGAGTCCCC ATCCCCTGCA 
CCCACACCCT CTCCAACACC AATGGAAGAT ATCAGTCCAC GGACAGTGAC ATCACCAGGC 
AGACTGTCCC TGTCAGAAAA GAAGTTTGAC TACCGACAAT TTGCTGCCAT TCCTTCTTCC 
AAACCCGTTT ACGACATTCA GTCTCCAGGT GCAGCTGATG ACGTGCACTT CATGGATGAG 
TCCTCCAGTA ACGCAGTGTC TGTTATTGAT CCAGAAGTTC CAGTGGCCGC CACGTCAGCT 
CTAGAAGAGA TGGCTCTCTA CAGTAACAAA CGTAAACTAC GCCAGGGCCG CCGCAGCCTG 
GAGGCTCCTG TGCCCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333090.1
CDS	1070..7210
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477415.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477415.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGGGTCTT CAGAGGAGAG CCACACCACA CCCTAGTGAG 
TTAAAGGTGA TGAAGAATGT AATTGAAGCA AGAAGAAATG AGGCTTATAC AGCCAGACCA 
GATGAAGATC TAGAATCCCC AGATGCTGAG GAGAAACGGT TAAGTGGTCT GTCCAATCAG 
AGTCATGATT CTCAGGCATC CAGTAGCACC ACCTCAGCCA CTTCCCATGA GGACAAGCAG 
GGTATAAACA GTACAGGGGT GGAGCTTAAT GATGGACAGG TTCAAGAAGA GGAGGATCTG 
GATGAGATGG AGGTGGAGTA TACTGAGCCT ACTGTGCACT TTGCTGAAGA GCCCATCATC 
CGTGGTGGTG ATGAGGACGA CGATTATGAT AATGACGATG ATGACGCTGA AAGGAGTGAC 
GAGGTGTCTC AAACGCCATC TTTTCCTGCC GAGAAACAGC GGCTCATACG TAAAGACACA 
CCACACTACA AAAAGCACTT CAAGATCACC AAGCTGCCCA AACCAGAGAC AGTCGCCGCC 
CTGCTGCAAG GCTTCAGTCA TGATGGCCTG TCACCTACTG CTCACACCCC TGAGAACGAG 
AGGGATGGAG AGGATGACGA GGAAGAGGAG GAGGATGAAG ACGAGGAGGA CGATCTCCAT 
ACTCCTTTCC AGCACAGACT AGATGCAGCA GAGCTAGAAG ACATCAGAAT GAGTCAGATG 
AACTCGTCCC TGCAGCCAGT GAAGGGGGTG TCATTTGATC AAGTCAATAA TCTGCTGATT 
GAACCTGCTC GAATTGAGGA GGAAGAGCTG ACCTTGAGCA TCCTGAGGCA GACTGGTGGA 
TTAGGCATCA GCATAGCAGG AGGGAAGGGA TCTACTCCCT ATAAAGGTGA CGATGAGGGT 
ATTTTCATCT CTAGGGTGTC AGAAGAAGGC CCTGCGGCTC GTGCTGGTGT CAAAGTTGGA 
GACAAACTTC TTGAGGTGAA TGGAGTAGAC CTGCACGGAG CGGAGCATCA TACAGCTGTT 
GAAGCTTTGC GCAATTCAGG AGCAGCCGTG GTCATGACTG TTTTGCGGGA GCGCATGGTG 
GAACCAGAAA ACGCCATCAC CACCACACCG TTGCGGCCAG AAGATGATTA TTTCCCCCGG 
GAGAGACGCT CCAGTGGGCT TCCTTTCCTC CTGGATCCTG ATTGTCCCGC TGTTAGCACT 
GGACCCGCAC AGCGACTAGC CACTTGTCTG ATCCGCAATG ATAAAGGCTT AGGCTTCAGC 
ATTGCTGGTG GGAAAGGGTC AACGCTGTAC AGAGTCGGAG ACACGGGCAT CTTCATCTCT 
CGTATTGCTG AAGGAGGAGC AGCTCACAGA GACAACATCC TGCAAGTGGG AGACAGGGTC 
ATATCAATCA ATGGTGTAGA CATGACAGAG GCCAGGCATG ACCAGGCAGT AGCGCTGCTT 
ACCGGCACCT CCCCCACCAT CACCCTGGTG GTGGATCGGG AACAAAGCAG TGTAGGCGGA 
GCCTCCCCTC GAACCCGACC ACATTCGCCC CCACCTCCAG AGCCCTCTGA CTCCCCTGAG 
CAAGAGGACG GTGGAGACGA GCACCTTGGA AACCATCTCA ACTGCCCCAT GGAAGATGAG 
TACCCTATTG AGGAAGTGAC GCTGATCAAA GCAGGTGGAC CCCTGGGTTT GAGCATTGTA 
GGGGGCAGCG ACCATGCCAG CCATCCATTT GGAATTAATG AACCTGGAGT GTTCATCTCA 
AAGGTCATCC CCAATGGCCT GGCCTCCCAG AGTGGACTTC GCGTGGGTGA TCGTATCTTG 
GAGGTGAACA GCATTGATCT GCGTCATGCC ACACACCAGG AGGCTGTTAG AGCCTTGCTT 
TCCAACAAAC AGGAGATCCG TATGCTTGTG CGCAGAGATC CTTCTCCCCC TGGCATGCAG 
GAAATTGTCA TTCACAAGCA ACCTGGAGAA AAGCTGGGAA TAAGCATTAG GGGCGGAGCT 
AAAGGTCATG CAGGAAACCC CTTTGACCCC ACAGATGAGG GCATCTTTAT TTCCAAGGTG 
AGCTCCAACG GTGCTGCTGC ACGAGATGGC CGACTGCGGG TGGGTATGCG TATTCTTGAG 
GTGGGCAACA ACAGTCTGCT GGGTATGACA CATACAGAAG CAGTGAGGGT CTTGCGTGCT 
TCTGGAGATT CCCTGGTCAT GCTTATTTGT GATGGCTTTG ACCCAAAAAG TGCTTCAACC 
ATAGAGGCAT CTCCTGGTGT TATTGCGAAT CCGTTTGCGG CAGGTATTGT TCGTAAAAAC 
AGTATGGAAA GCATCTCATC AGTCGACAGA GACCTGAGTC CAGAAGAGAT TGACATCATG 
CAGAAGGAGG TAGAAATGGT AAGAGAGACG TCCCAGTGGG AACGAGAGGA GATGGAAAAA 
GTGGAGCGCA TGCGCTTGGA GCGCGAGGAG GCAACTCGCT TGCTGGAGGA GGAAACTGAG 
AGTTTGAGCT CTGGACCACT GAAACTGGAC TATAAGACAC TGGCGGCTCT GCCTACAACT 
AGTCTACAGA AAGTTAATAG ATTTTCTCCC TCCTTTTCCA CATTTGCCAA AATGGAGGCT 
GCCATGCCGG CTGGCCCCGT AGACCCCCTG GGTTTTGAGA AAGTCCATGC TTCTATGTAC 
CATGTAGAAC CTGTTGCCCT CCCTTCATCT CCAATCTGCC CAGCCGCAAG TTCAGCGGCT 
TCAAAACAGC CCCAAGCTGA CTCACGGGAC CTGGTCTTAG ATCTAGGAAT GGCTCAAGAG 
GAGGAGGAAT GTCTTGTGGA CTCCCAACCA ATCTGCTTTA GTGAAAACCC ATTTCTTGTG 
GCTAACCGCA AGGGCAAAGG CTGCCCACCA GAAGAGCGCA TTTTATCAGG GCCACCTGTG 
GGTTACGGCA AGCATGGGCA GCTGCAGCCC TGGTTGTACA GCAAGGCTTC ACCATCTGAG 
CCTTTCAGGA TTGACAGTCC TGTCCGAGAT GCTGCACACT CTCCACACAA CTCACAGCAG 
TCTCACAAAT CTTCCTCTGC GATGCTTGGA AGAGAGGTTC GATATTCAAA CATTCATTTC 
CCTTCAAATG CAAACACCAA GGATAATGCA TCAACTAAAC CTGGTGCCAT CCAGCCTCTC 
TCTCGGGTGC GACCCCCTGT GTCTCCAGCC AGTCAGGATG GCCACAGTAG TCCAAACCCC 
TTCCAGCATG GCCTCTCTCC CATCAACTCT CAGACCACTC CTCGTGCCCC ATCCCCTGAC 
ACACATGAGG AATACCCTAT CAATATTAAG CAGGTTTATA AGGCCTTCGC TGCTGTGCCA 
CACTCCCTGG CCGTACTGGA ACCCCCTCAG GATCTTTATT CTCCACGGAA CAATGTCAGT 
GCAAAACAGC CATCACCTGA GTTTCAGCCT GAGCTGAATA ATGAAGTGTT TATGGATGAG 
TGTGATGGTG AGGGCAGTGG TCCTCGTCCC TCCCTCTTAT CTGACCGCAG CGAGTACAGA 
AGCCTGGCAG CTGTGCCACG CCTGTCTCGC CCTTCAGTGG AGCCAAAGAC CCCTTCGCCT 
CTGGGTAGGC ACAGTCCAGA ACAGCGCTCC TTCAAAGACA GACAAAAGTA TTTCGAGATC 
GATGTGAAGC AACAGACTCC AGACAAACCC AAACCTCGCA TCTCACTGGT GGGAGAAGAT 
GACCTGAAGA AAATGAAGGA GGAAGAAGCA AAGCGAATTG AACAGAGGGC GCGAGAGTTC 
ATGTTGGATG AAGATGAGGA GGAGGAAGAA GAGGATCTTG CCAAGCAAGT GGCACATATG 
AAGGCTCACG GAAAGGTTGT TCTTGATGGT GTGGAGTACA AGGTAGAGAG CTTGGGTTCC 
CCGACTTCAC GGCAGTGTGC CACACCACCA AACTATAGTG CCACACCACC CAGCCACTGC 
GGAAGCTCAG GTCCTTCCTC TATCGACGGT AAAGGAGACT CCCAGCGTAA TTCAGTGGAA 
GACAGTTTCA GACTGGAGCA GAGACCCAAT TCTATGACCG GATTGATCCC AGTTTATCCC 
GGTGAATCTG CGGCTCCCAT CCGGACGGCC AAAGCCGAGC GCAGGCATCA AGATCGATTA 
CGCATGCAGA GCCCAGAGCT CACTGTCGCT TTGGACAAGG AGCTGTCGCC TGCTGAAAAA 
CGAGCCCTGG AGGCAGAGAA GAGAGCCATG TGGAGAGCAG CCAGGCCTTA TGGCTTAGAG 
GAGGACGTAA GGCAGTATGA GCAAGACCTG GCTAAGCGGC TCTACCAGTC CCGTGTGAGG 
GCATCACTGG GCAGCGCTGC AGCCCCTGCC TCTTCCTCCT GCTCCTCTTC CTCTTCCTGT 
CAGCCCAGGA TGAAGTCTTT AGAGCAGGAC GCTTTGAAGG CTCAGATGGT CATTGCCAAG 
TCTAAAGAGG GCAAGAAGCG AGGAACCCTG GATCAGCTGT CAGAGTCCCC ATCCCCTGCA 
CCCACACCCT CTCCAACACC AATGGAAGAT ATCAGTCCAC GGACAGTGAC ATCACCAGGC 
AGACTGTCCC TGTCAGAAAA GAAGTTTGAC TACCGACAAT TTGCTGCCAT TCCTTCTTCC 
AAACCCGTTT ACGACATTCA GTCTCCAGGT GCAGCTGATG ACGTGCACTT CATGGATGAG 
TCCTCCAGTA ACGCAGTGTC TGTTATTGAT CCAGAAGTTC CAGTGGCCGC CACGTCAGCT 
CTAGAAGAGA TGGCTCTCTA CAGTAACAAA CGTAAACTAC GCCAGGGCCG CCGCAGCCTG 
GAGGCTCCTG TGCCCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477416.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333091.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X3, mRNA.

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CloneID	ODa143210
Clone ID Related Accession (Same CDS sequence)	XM_021477416.1
Accession Version	XM_021477416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
TCAAACATTC ATTTCCCTTC AAATGCAAAC ACCAAGGATA ATGCATCAAC TAAACCTGGT 
GCCATCCAGC CTCTCTCTCG GGTGCGACCC CCTGTGTCTC CAGCCAGTCA GGATGGCCAC 
AGTAGTCCAA ACCCCTTCCA GCATGGCCTC TCTCCCATCA ACTCTCAGAC CACTCCTCGT 
GCCCCATCCC CTGACACACA TGAGGAATAC CCTATCAATA TTAAGCAGGT TTATAAGGCC 
TTCGCTGCTG TGCCACACTC CCTGGCCGTA CTGGAACCCC CTCAGGATCT TTATTCTCCA 
CGGAACAATG TCAGTGCAAA ACAGCCATCA CCTGAGTTTC AGCCTGAGCT GAATAATGAA 
GTGTTTATGG ATGAGTGTGA TGGTGAGGGC AGTGGTCCTC GTCCCTCCCT CTTATCTGAC 
CGCAGCGAGT ACAGAAGCCT GGCAGCTGTG CCACGCCTGT CTCGCCCTTC AGTGGAGCCA 
AAGACCCCTT CGCCTCTGGG TAGGCACAGT CCAGAACAGC GCTCCTTCAA AGACAGACAA 
AAGTATTTCG AGATCGATGT GAAGCAACAG ACTCCAGACA AACCCAAACC TCGCATCTCA 
CTGGTGGGAG AAGATGACCT GAAGAAAATG AAGGAGGAAG AAGCAAAGCG AATTGAACAG 
AGGGCGCGAG AGTTCATGTT GGATGAAGAT GAGGAGGAGG AAGAAGAGGA TCTTGCCAAG 
CAAGTGGCAC ATATGAAGGC TCACGGAAAG GTTGTTCTTG ATGGTGTGGA GTACAAGGTA 
GAGAGCTTGG GTTCCCCGAC TTCACGGCAG TGTGCCACAC CACCAAACTA TAGTGCCACA 
CCACCCAGCC ACTGCGGAAG CTCAGGTCCT TCCTCTATCG ACGGTAAAGG AGACTCCCAG 
CGTAATTCAG TGGAAGACAG TTTCAGACTG GAGCAGAGAC CCAATTCTAT GACCGGATTG 
ATCCCAGTTT ATCCCGGTGA ATCTGCGGCT CCCATCCGGA CGGCCAAAGC CGAGCGCAGG 
CATCAAGATC GATTACGCAT GCAGAGCCCA GAGCTCACTG TCGCTTTGGA CAAGGAGCTG 
TCGCCTGCTG AAAAACGAGC CCTGGAGGCA GAGAAGAGAG CCATGTGGAG AGCAGCCAGG 
CCTTATGGCT TAGAGGAGGA CGTAAGGCAG TATGAGCAAG ACCTGGCTAA GCGGCTCTAC 
CAGTCCCGTG TGAGGGCATC ACTGGGCAGC GCTGCAGCCC CTGCCTCTTC CTCCTGCTCC 
TCTTCCTCTT CCTGTCAGCC CAGGATGAAG TCTTTAGAGC AGGACGCTTT GAAGGCTCAG 
ATGGTCATTG CCAAGTCTAA AGAGGGCAAG AAGCGAGGAA CCCTGGATCA GCTGTCAGAG 
TCCCCATCCC CTGCACCCAC ACCCTCTCCA ACACCAATGG AAGATATCAG TCCACGGACA 
GTGACATCAC CAGGCAGACT GTCCCTGTCA GAAAAGAAGT TTGACTACCG ACAATTTGCT 
GCCATTCCTT CTTCCAAACC CGTTTACGAC ATTCAGTCTC CAGGTGCAGC TGATGACGTG 
CACTTCATGG ATGAGTCCTC CAGTAACGCA GTGTCTGTTA TTGATCCAGA AGTTCCAGTG 
GCCGCCACGT CAGCTCTAGA AGAGATGGCT CTCTACAGTA ACAAACGTAA ACTACGCCAG 
GGCCGCCGCA GCCTGGAGGC TCCTGTGCCC ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333091.1
CDS	1070..7165
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477416.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477416.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
TCAAACATTC ATTTCCCTTC AAATGCAAAC ACCAAGGATA ATGCATCAAC TAAACCTGGT 
GCCATCCAGC CTCTCTCTCG GGTGCGACCC CCTGTGTCTC CAGCCAGTCA GGATGGCCAC 
AGTAGTCCAA ACCCCTTCCA GCATGGCCTC TCTCCCATCA ACTCTCAGAC CACTCCTCGT 
GCCCCATCCC CTGACACACA TGAGGAATAC CCTATCAATA TTAAGCAGGT TTATAAGGCC 
TTCGCTGCTG TGCCACACTC CCTGGCCGTA CTGGAACCCC CTCAGGATCT TTATTCTCCA 
CGGAACAATG TCAGTGCAAA ACAGCCATCA CCTGAGTTTC AGCCTGAGCT GAATAATGAA 
GTGTTTATGG ATGAGTGTGA TGGTGAGGGC AGTGGTCCTC GTCCCTCCCT CTTATCTGAC 
CGCAGCGAGT ACAGAAGCCT GGCAGCTGTG CCACGCCTGT CTCGCCCTTC AGTGGAGCCA 
AAGACCCCTT CGCCTCTGGG TAGGCACAGT CCAGAACAGC GCTCCTTCAA AGACAGACAA 
AAGTATTTCG AGATCGATGT GAAGCAACAG ACTCCAGACA AACCCAAACC TCGCATCTCA 
CTGGTGGGAG AAGATGACCT GAAGAAAATG AAGGAGGAAG AAGCAAAGCG AATTGAACAG 
AGGGCGCGAG AGTTCATGTT GGATGAAGAT GAGGAGGAGG AAGAAGAGGA TCTTGCCAAG 
CAAGTGGCAC ATATGAAGGC TCACGGAAAG GTTGTTCTTG ATGGTGTGGA GTACAAGGTA 
GAGAGCTTGG GTTCCCCGAC TTCACGGCAG TGTGCCACAC CACCAAACTA TAGTGCCACA 
CCACCCAGCC ACTGCGGAAG CTCAGGTCCT TCCTCTATCG ACGGTAAAGG AGACTCCCAG 
CGTAATTCAG TGGAAGACAG TTTCAGACTG GAGCAGAGAC CCAATTCTAT GACCGGATTG 
ATCCCAGTTT ATCCCGGTGA ATCTGCGGCT CCCATCCGGA CGGCCAAAGC CGAGCGCAGG 
CATCAAGATC GATTACGCAT GCAGAGCCCA GAGCTCACTG TCGCTTTGGA CAAGGAGCTG 
TCGCCTGCTG AAAAACGAGC CCTGGAGGCA GAGAAGAGAG CCATGTGGAG AGCAGCCAGG 
CCTTATGGCT TAGAGGAGGA CGTAAGGCAG TATGAGCAAG ACCTGGCTAA GCGGCTCTAC 
CAGTCCCGTG TGAGGGCATC ACTGGGCAGC GCTGCAGCCC CTGCCTCTTC CTCCTGCTCC 
TCTTCCTCTT CCTGTCAGCC CAGGATGAAG TCTTTAGAGC AGGACGCTTT GAAGGCTCAG 
ATGGTCATTG CCAAGTCTAA AGAGGGCAAG AAGCGAGGAA CCCTGGATCA GCTGTCAGAG 
TCCCCATCCC CTGCACCCAC ACCCTCTCCA ACACCAATGG AAGATATCAG TCCACGGACA 
GTGACATCAC CAGGCAGACT GTCCCTGTCA GAAAAGAAGT TTGACTACCG ACAATTTGCT 
GCCATTCCTT CTTCCAAACC CGTTTACGAC ATTCAGTCTC CAGGTGCAGC TGATGACGTG 
CACTTCATGG ATGAGTCCTC CAGTAACGCA GTGTCTGTTA TTGATCCAGA AGTTCCAGTG 
GCCGCCACGT CAGCTCTAGA AGAGATGGCT CTCTACAGTA ACAAACGTAA ACTACGCCAG 
GGCCGCCGCA GCCTGGAGGC TCCTGTGCCC ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477417.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333092.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X4, mRNA.

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CloneID	ODa143211
Clone ID Related Accession (Same CDS sequence)	XM_021477417.1
Accession Version	XM_021477417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGAGTT TGAGCTCTGG ACCACTGAAA CTGGACTATA AGACACTGGC GGCTCTGCCT 
ACAACTAGTC TACAGAAAGT TAATAGATTT TCTCCCTCCT TTTCCACATT TGCCAAAATG 
GAGGCTGCCA TGCCGGCTGG CCCCGTAGAC CCCCTGGGTT TTGAGAAAGT CCATGCTTCT 
ATGTACCATG TAGAACCTGT TGCCCTCCCT TCATCTCCAA TCTGCCCAGC CGCAAGTTCA 
GCGGCTTCAA AACAGCCCCA AGCTGACTCA CGGGACCTGG TCTTAGATCT AGGAATGGCT 
CAAGAGGAGG AGGAATGTCT TGTGGACTCC CAACCAATCT GCTTTAGTGA AAACCCATTT 
CTTGTGGCTA ACCGCAAGGG CAAAGGCTGC CCACCAGAAG AGCGCATTTT ATCAGGGCCA 
CCTGTGGGTT ACGGCAAGCA TGGGCAGCTG CAGCCCTGGT TGTACAGCAA GGCTTCACCA 
TCTGAGCCTT TCAGGATTGA CAGTCCTGTC CGAGATGCTG CACACTCTCC ACACAACTCA 
CAGCAGTCTC ACAAATCTTC CTCTGCGATG CTTGGAAGAG AGGTTCGATA TTCAAACATT 
CATTTCCCTT CAAATGCAAA CACCAAGGAT AATGCATCAA CTAAACCTGG TGCCATCCAG 
CCTCTCTCTC GGGTGCGACC CCCTGTGTCT CCAGCCAGTC AGGATGGCCA CAGTAGTCCA 
AACCCCTTCC AGCATGGCCT CTCTCCCATC AACTCTCAGA CCACTCCTCG TGCCCCATCC 
CCTGACACAC ATGAGGAATA CCCTATCAAT ATTAAGCAGG TTTATAAGGC CTTCGCTGCT 
GTGCCACACT CCCTGGCCGT ACTGGAACCC CCTCAGGATC TTTATTCTCC ACGGAACAAT 
GTCAGTGCAA AACAGCCATC ACCTGAGTTT CAGCCTGAGC TGAATAATGA AGTGTTTATG 
GATGAGTGTG ATGGTGAGGG CAGTGGTCCT CGTCCCTCCC TCTTATCTGA CCGCAGCGAG 
TACAGAAGCC TGGCAGCTGT GCCACGCCTG TCTCGCCCTT CAGTGGAGCC AAAGACCCCT 
TCGCCTCTGG GTAGGCACAG TCCAGAACAG CGCTCCTTCA AAGACAGACA AAAGTATTTC 
GAGATCGATG TGAAGCAACA GACTCCAGAC AAACCCAAAC CTCGCATCTC ACTGGTGGGA 
GAAGATGACC TGAAGAAAAT GAAGGAGGAA GAAGCAAAGC GAATTGAACA GAGGGCGCGA 
GAGTTCATGT TGGATGAAGA TGAGGAGGAG GAAGAAGAGG ATCTTGCCAA GCAAGTGGCA 
CATATGAAGG CTCACGGAAA GGTTGTTCTT GATGGTGTGG AGTACAAGGT AGAGAGCTTG 
GGTTCCCCGA CTTCACGGCA GTGTGCCACA CCACCAAACT ATAGTGCCAC ACCACCCAGC 
CACTGCGGAA GCTCAGGTCC TTCCTCTATC GACGGTAAAG GAGACTCCCA GCGTAATTCA 
GTGGAAGACA GTTTCAGACT GGAGCAGAGA CCCAATTCTA TGACCGGATT GATCCCAGTT 
TATCCCGGTG AATCTGCGGC TCCCATCCGG ACGGCCAAAG CCGAGCGCAG GCATCAAGAT 
CGATTACGCA TGCAGAGCCC AGAGCTCACT GTCGCTTTGG ACAAGGAGCT GTCGCCTGCT 
GAAAAACGAG CCCTGGAGGC AGAGAAGAGA GCCATGTGGA GAGCAGCCAG GCCTTATGGC 
TTAGAGGAGG ACGTAAGGCA GTATGAGCAA GACCTGGCTA AGCGGCTCTA CCAGTCCCGT 
GTGAGGGCAT CACTGGGCAG CGCTGCAGCC CCTGCCTCTT CCTCCTGCTC CTCTTCCTCT 
TCCTGTCAGC CCAGGATGAA GTCTTTAGAG CAGGACGCTT TGAAGGCTCA GATGGTCATT 
GCCAAGTCTA AAGAGGGCAA GAAGCGAGGA ACCCTGGATC AGCTGTCAGA GTCCCCATCC 
CCTGCACCCA CACCCTCTCC AACACCAATG GAAGATATCA GTCCACGGAC AGTGACATCA 
CCAGGCAGAC TGTCCCTGTC AGAAAAGAAG TTTGACTACC GACAATTTGC TGCCATTCCT 
TCTTCCAAAC CCGTTTACGA CATTCAGTCT CCAGGTGCAG CTGATGACGT GCACTTCATG 
GATGAGTCCT CCAGTAACGC AGTGTCTGTT ATTGATCCAG AAGTTCCAGT GGCCGCCACG 
TCAGCTCTAG AAGAGATGGC TCTCTACAGT AACAAACGTA AACTACGCCA GGGCCGCCGC 
AGCCTGGAGG CTCCTGTGCC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333092.1
CDS	1070..7156
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVSLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477417.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477417.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGAGTT TGAGCTCTGG ACCACTGAAA CTGGACTATA AGACACTGGC GGCTCTGCCT 
ACAACTAGTC TACAGAAAGT TAATAGATTT TCTCCCTCCT TTTCCACATT TGCCAAAATG 
GAGGCTGCCA TGCCGGCTGG CCCCGTAGAC CCCCTGGGTT TTGAGAAAGT CCATGCTTCT 
ATGTACCATG TAGAACCTGT TGCCCTCCCT TCATCTCCAA TCTGCCCAGC CGCAAGTTCA 
GCGGCTTCAA AACAGCCCCA AGCTGACTCA CGGGACCTGG TCTTAGATCT AGGAATGGCT 
CAAGAGGAGG AGGAATGTCT TGTGGACTCC CAACCAATCT GCTTTAGTGA AAACCCATTT 
CTTGTGGCTA ACCGCAAGGG CAAAGGCTGC CCACCAGAAG AGCGCATTTT ATCAGGGCCA 
CCTGTGGGTT ACGGCAAGCA TGGGCAGCTG CAGCCCTGGT TGTACAGCAA GGCTTCACCA 
TCTGAGCCTT TCAGGATTGA CAGTCCTGTC CGAGATGCTG CACACTCTCC ACACAACTCA 
CAGCAGTCTC ACAAATCTTC CTCTGCGATG CTTGGAAGAG AGGTTCGATA TTCAAACATT 
CATTTCCCTT CAAATGCAAA CACCAAGGAT AATGCATCAA CTAAACCTGG TGCCATCCAG 
CCTCTCTCTC GGGTGCGACC CCCTGTGTCT CCAGCCAGTC AGGATGGCCA CAGTAGTCCA 
AACCCCTTCC AGCATGGCCT CTCTCCCATC AACTCTCAGA CCACTCCTCG TGCCCCATCC 
CCTGACACAC ATGAGGAATA CCCTATCAAT ATTAAGCAGG TTTATAAGGC CTTCGCTGCT 
GTGCCACACT CCCTGGCCGT ACTGGAACCC CCTCAGGATC TTTATTCTCC ACGGAACAAT 
GTCAGTGCAA AACAGCCATC ACCTGAGTTT CAGCCTGAGC TGAATAATGA AGTGTTTATG 
GATGAGTGTG ATGGTGAGGG CAGTGGTCCT CGTCCCTCCC TCTTATCTGA CCGCAGCGAG 
TACAGAAGCC TGGCAGCTGT GCCACGCCTG TCTCGCCCTT CAGTGGAGCC AAAGACCCCT 
TCGCCTCTGG GTAGGCACAG TCCAGAACAG CGCTCCTTCA AAGACAGACA AAAGTATTTC 
GAGATCGATG TGAAGCAACA GACTCCAGAC AAACCCAAAC CTCGCATCTC ACTGGTGGGA 
GAAGATGACC TGAAGAAAAT GAAGGAGGAA GAAGCAAAGC GAATTGAACA GAGGGCGCGA 
GAGTTCATGT TGGATGAAGA TGAGGAGGAG GAAGAAGAGG ATCTTGCCAA GCAAGTGGCA 
CATATGAAGG CTCACGGAAA GGTTGTTCTT GATGGTGTGG AGTACAAGGT AGAGAGCTTG 
GGTTCCCCGA CTTCACGGCA GTGTGCCACA CCACCAAACT ATAGTGCCAC ACCACCCAGC 
CACTGCGGAA GCTCAGGTCC TTCCTCTATC GACGGTAAAG GAGACTCCCA GCGTAATTCA 
GTGGAAGACA GTTTCAGACT GGAGCAGAGA CCCAATTCTA TGACCGGATT GATCCCAGTT 
TATCCCGGTG AATCTGCGGC TCCCATCCGG ACGGCCAAAG CCGAGCGCAG GCATCAAGAT 
CGATTACGCA TGCAGAGCCC AGAGCTCACT GTCGCTTTGG ACAAGGAGCT GTCGCCTGCT 
GAAAAACGAG CCCTGGAGGC AGAGAAGAGA GCCATGTGGA GAGCAGCCAG GCCTTATGGC 
TTAGAGGAGG ACGTAAGGCA GTATGAGCAA GACCTGGCTA AGCGGCTCTA CCAGTCCCGT 
GTGAGGGCAT CACTGGGCAG CGCTGCAGCC CCTGCCTCTT CCTCCTGCTC CTCTTCCTCT 
TCCTGTCAGC CCAGGATGAA GTCTTTAGAG CAGGACGCTT TGAAGGCTCA GATGGTCATT 
GCCAAGTCTA AAGAGGGCAA GAAGCGAGGA ACCCTGGATC AGCTGTCAGA GTCCCCATCC 
CCTGCACCCA CACCCTCTCC AACACCAATG GAAGATATCA GTCCACGGAC AGTGACATCA 
CCAGGCAGAC TGTCCCTGTC AGAAAAGAAG TTTGACTACC GACAATTTGC TGCCATTCCT 
TCTTCCAAAC CCGTTTACGA CATTCAGTCT CCAGGTGCAG CTGATGACGT GCACTTCATG 
GATGAGTCCT CCAGTAACGC AGTGTCTGTT ATTGATCCAG AAGTTCCAGT GGCCGCCACG 
TCAGCTCTAG AAGAGATGGC TCTCTACAGT AACAAACGTA AACTACGCCA GGGCCGCCGC 
AGCCTGGAGG CTCCTGTGCC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477418.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333093.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X5, mRNA.

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CloneID	ODa143212
Clone ID Related Accession (Same CDS sequence)	XM_021477418.1
Accession Version	XM_021477418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGCTG ACCTTGAGCA TCCTGAGGCA GACTGGTGGA 
TTAGGCATCA GCATAGCAGG AGGGAAGGGA TCTACTCCCT ATAAAGGTGA CGATGAGGGT 
ATTTTCATCT CTAGGGTGTC AGAAGAAGGC CCTGCGGCTC GTGCTGGTGT CAAAGTTGGA 
GACAAACTTC TTGAGGTGAA TGGAGTAGAC CTGCACGGAG CGGAGCATCA TACAGCTGTT 
GAAGCTTTGC GCAATTCAGG AGCAGCCGTG GTCATGACTG TTTTGCGGGA GCGCATGGTG 
GAACCAGAAA ACGCCATCAC CACCACACCG TTGCGGCCAG AAGATGATTA TTTCCCCCGG 
GAGAGACGCT CCAGTGGGCT TCCTTTCCTC CTGGATCCTG ATTGTCCCGC TGTTAGCACT 
GGACCCGCAC AGCGACTAGC CACTTGTCTG ATCCGCAATG ATAAAGGCTT AGGCTTCAGC 
ATTGCTGGTG GGAAAGGGTC AACGCTGTAC AGAGTCGGAG ACACGGGCAT CTTCATCTCT 
CGTATTGCTG AAGGAGGAGC AGCTCACAGA GACAACATCC TGCAAGTGGG AGACAGGGTC 
ATATCAATCA ATGGTGTAGA CATGACAGAG GCCAGGCATG ACCAGGCAGT AGCGCTGCTT 
ACCGGCACCT CCCCCACCAT CACCCTGGTG GTGGATCGGG AACAAAGCAG TGTAGGCGGA 
GCCTCCCCTC GAACCCGACC ACATTCGCCC CCACCTCCAG AGCCCTCTGA CTCCCCTGAG 
CAAGAGGACG GTGGAGACGA GCACCTTGGA AACCATCTCA ACTGCCCCAT GGAAGATGAG 
TACCCTATTG AGGAAGTGAC GCTGATCAAA GCAGGTGGAC CCCTGGGTTT GAGCATTGTA 
GGGGGCAGCG ACCATGCCAG CCATCCATTT GGAATTAATG AACCTGGAGT GTTCATCTCA 
AAGGTCATCC CCAATGGCCT GGCCTCCCAG AGTGGACTTC GCGTGGGTGA TCGTATCTTG 
GAGGTGAACA GCATTGATCT GCGTCATGCC ACACACCAGG AGGCTGTTAG AGCCTTGCTT 
TCCAACAAAC AGGAGATCCG TATGCTTGTG CGCAGAGATC CTTCTCCCCC TGGCATGCAG 
GAAATTGTCA TTCACAAGCA ACCTGGAGAA AAGCTGGGAA TAAGCATTAG GGGCGGAGCT 
AAAGGTCATG CAGGAAACCC CTTTGACCCC ACAGATGAGG GCATCTTTAT TTCCAAGGTG 
AGCTCCAACG GTGCTGCTGC ACGAGATGGC CGACTGCGGG TGGGTATGCG TATTCTTGAG 
GTGGGCAACA ACAGTCTGCT GGGTATGACA CATACAGAAG CAGTGAGGGT CTTGCGTGCT 
TCTGGAGATT CCCTGGTCAT GCTTATTTGT GATGGCTTTG ACCCAAAAAG TGCTTCAACC 
ATAGAGGCAT CTCCTGGTGT TATTGCGAAT CCGTTTGCGG CAGGTATTGT TCGTAAAAAC 
AGTATGGAAA GCATCTCATC AGTCGACAGA GACCTGAGTC CAGAAGAGAT TGACATCATG 
CAGAAGGAGG TAGAAATGGT AAGAGAGACG TCCCAGTGGG AACGAGAGGA GATGGAAAAA 
GTGGAGCGCA TGCGCTTGGA GCGCGAGGAG GCAACTCGCT TGCTGGAGGA GGAAACTGAG 
AGTTTGAGCT CTGGACCACT GAAACTGGAC TATAAGACAC TGGCGGCTCT GCCTACAACT 
AGTCTACAGA AAGTTAATAG ATTTTCTCCC TCCTTTTCCA CATTTGCCAA AATGGAGGCT 
GCCATGCCGG CTGGCCCCGT AGACCCCCTG GGTTTTGAGA AAGTCCATGC TTCTATGTAC 
CATGTAGAAC CTGTTGCCCT CCCTTCATCT CCAATCTGCC CAGCCGCAAG TTCAGCGGCT 
TCAAAACAGC CCCAAGCTGA CTCACGGGAC CTGGTCTTAG ATCTAGGAAT GGCTCAAGAG 
GAGGAGGAAT GTCTTGTGGA CTCCCAACCA ATCTGCTTTA GTGAAAACCC ATTTCTTGTG 
GCTAACCGCA AGGGCAAAGG CTGCCCACCA GAAGAGCGCA TTTTATCAGG GCCACCTGTG 
GGTTACGGCA AGCATGGGCA GCTGCAGCCC TGGTTGTACA GCAAGGCTTC ACCATCTGAG 
CCTTTCAGGA TTGACAGTCC TGTCCGAGAT GCTGCACACT CTCCACACAA CTCACAGCAG 
TCTCACAAAT CTTCCTCTGC GATGCTTGGA AGAGAGGTTC GATATTCAAA CATTCATTTC 
CCTTCAAATG CAAACACCAA GGATAATGCA TCAACTAAAC CTGGTGCCAT CCAGCCTCTC 
TCTCGGGTGC GACCCCCTGT GTCTCCAGCC AGTCAGGATG GCCACAGTAG TCCAAACCCC 
TTCCAGCATG GCCTCTCTCC CATCAACTCT CAGACCACTC CTCGTGCCCC ATCCCCTGAC 
ACACATGAGG AATACCCTAT CAATATTAAG CAGGTTTATA AGGCCTTCGC TGCTGTGCCA 
CACTCCCTGG CCGTACTGGA ACCCCCTCAG GATCTTTATT CTCCACGGAA CAATGTCAGT 
GCAAAACAGC CATCACCTGA GTTTCAGCCT GAGCTGAATA ATGAAGTGTT TATGGATGAG 
TGTGATGGTG AGGGCAGTGG TCCTCGTCCC TCCCTCTTAT CTGACCGCAG CGAGTACAGA 
AGCCTGGCAG CTGTGCCACG CCTGTCTCGC CCTTCAGTGG AGCCAAAGAC CCCTTCGCCT 
CTGGGTAGGC ACAGTCCAGA ACAGCGCTCC TTCAAAGACA GACAAAAGTA TTTCGAGATC 
GATGTGAAGC AACAGACTCC AGACAAACCC AAACCTCGCA TCTCACTGGT GGGAGAAGAT 
GACCTGAAGA AAATGAAGGA GGAAGAAGCA AAGCGAATTG AACAGAGGGC GCGAGAGTTC 
ATGTTGGATG AAGATGAGGA GGAGGAAGAA GAGGATCTTG CCAAGCAAGT GGCACATATG 
AAGGCTCACG GAAAGGTTGT TCTTGATGGT GTGGAGTACA AGGTAGAGAG CTTGGGTTCC 
CCGACTTCAC GGCAGTGTGC CACACCACCA AACTATAGTG CCACACCACC CAGCCACTGC 
GGAAGCTCAG GTCCTTCCTC TATCGACGGT AAAGGAGACT CCCAGCGTAA TTCAGTGGAA 
GACAGTTTCA GACTGGAGCA GAGACCCAAT TCTATGACCG GATTGATCCC AGTTTATCCC 
GGTGAATCTG CGGCTCCCAT CCGGACGGCC AAAGCCGAGC GCAGGCATCA AGATCGATTA 
CGCATGCAGA GCCCAGAGCT CACTGTCGCT TTGGACAAGG AGCTGTCGCC TGCTGAAAAA 
CGAGCCCTGG AGGCAGAGAA GAGAGCCATG TGGAGAGCAG CCAGGCCTTA TGGCTTAGAG 
GAGGACGTAA GGCAGTATGA GCAAGACCTG GCTAAGCGGC TCTACCAGTC CCGTGTGAGG 
GCATCACTGG GCAGCGCTGC AGCCCCTGCC TCTTCCTCCT GCTCCTCTTC CTCTTCCTGT 
CAGCCCAGGA TGAAGTCTTT AGAGCAGGAC GCTTTGAAGG CTCAGATGGT CATTGCCAAG 
TCTAAAGAGG GCAAGAAGCG AGGAACCCTG GATCAGCTGT CAGAGTCCCC ATCCCCTGCA 
CCCACACCCT CTCCAACACC AATGGAAGAT ATCAGTCCAC GGACAGTGAC ATCACCAGGC 
AGACTGTCCC TGTCAGAAAA GAAGTTTGAC TACCGACAAT TTGCTGCCAT TCCTTCTTCC 
AAACCCGTTT ACGACATTCA GTCTCCAGGT GCAGCTGATG ACGTGCACTT CATGGATGAG 
TCCTCCAGTA ACGCAGTGTC TGTTATTGAT CCAGAAGTTC CAGTGGCCGC CACGTCAGCT 
CTAGAAGAGA TGGCTCTCTA CAGTAACAAA CGTAAACTAC GCCAGGGCCG CCGCAGCCTG 
GAGGCTCCTG TGCCCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333093.1
CDS	1070..7150
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKLTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477418.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477418.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGCTG ACCTTGAGCA TCCTGAGGCA GACTGGTGGA 
TTAGGCATCA GCATAGCAGG AGGGAAGGGA TCTACTCCCT ATAAAGGTGA CGATGAGGGT 
ATTTTCATCT CTAGGGTGTC AGAAGAAGGC CCTGCGGCTC GTGCTGGTGT CAAAGTTGGA 
GACAAACTTC TTGAGGTGAA TGGAGTAGAC CTGCACGGAG CGGAGCATCA TACAGCTGTT 
GAAGCTTTGC GCAATTCAGG AGCAGCCGTG GTCATGACTG TTTTGCGGGA GCGCATGGTG 
GAACCAGAAA ACGCCATCAC CACCACACCG TTGCGGCCAG AAGATGATTA TTTCCCCCGG 
GAGAGACGCT CCAGTGGGCT TCCTTTCCTC CTGGATCCTG ATTGTCCCGC TGTTAGCACT 
GGACCCGCAC AGCGACTAGC CACTTGTCTG ATCCGCAATG ATAAAGGCTT AGGCTTCAGC 
ATTGCTGGTG GGAAAGGGTC AACGCTGTAC AGAGTCGGAG ACACGGGCAT CTTCATCTCT 
CGTATTGCTG AAGGAGGAGC AGCTCACAGA GACAACATCC TGCAAGTGGG AGACAGGGTC 
ATATCAATCA ATGGTGTAGA CATGACAGAG GCCAGGCATG ACCAGGCAGT AGCGCTGCTT 
ACCGGCACCT CCCCCACCAT CACCCTGGTG GTGGATCGGG AACAAAGCAG TGTAGGCGGA 
GCCTCCCCTC GAACCCGACC ACATTCGCCC CCACCTCCAG AGCCCTCTGA CTCCCCTGAG 
CAAGAGGACG GTGGAGACGA GCACCTTGGA AACCATCTCA ACTGCCCCAT GGAAGATGAG 
TACCCTATTG AGGAAGTGAC GCTGATCAAA GCAGGTGGAC CCCTGGGTTT GAGCATTGTA 
GGGGGCAGCG ACCATGCCAG CCATCCATTT GGAATTAATG AACCTGGAGT GTTCATCTCA 
AAGGTCATCC CCAATGGCCT GGCCTCCCAG AGTGGACTTC GCGTGGGTGA TCGTATCTTG 
GAGGTGAACA GCATTGATCT GCGTCATGCC ACACACCAGG AGGCTGTTAG AGCCTTGCTT 
TCCAACAAAC AGGAGATCCG TATGCTTGTG CGCAGAGATC CTTCTCCCCC TGGCATGCAG 
GAAATTGTCA TTCACAAGCA ACCTGGAGAA AAGCTGGGAA TAAGCATTAG GGGCGGAGCT 
AAAGGTCATG CAGGAAACCC CTTTGACCCC ACAGATGAGG GCATCTTTAT TTCCAAGGTG 
AGCTCCAACG GTGCTGCTGC ACGAGATGGC CGACTGCGGG TGGGTATGCG TATTCTTGAG 
GTGGGCAACA ACAGTCTGCT GGGTATGACA CATACAGAAG CAGTGAGGGT CTTGCGTGCT 
TCTGGAGATT CCCTGGTCAT GCTTATTTGT GATGGCTTTG ACCCAAAAAG TGCTTCAACC 
ATAGAGGCAT CTCCTGGTGT TATTGCGAAT CCGTTTGCGG CAGGTATTGT TCGTAAAAAC 
AGTATGGAAA GCATCTCATC AGTCGACAGA GACCTGAGTC CAGAAGAGAT TGACATCATG 
CAGAAGGAGG TAGAAATGGT AAGAGAGACG TCCCAGTGGG AACGAGAGGA GATGGAAAAA 
GTGGAGCGCA TGCGCTTGGA GCGCGAGGAG GCAACTCGCT TGCTGGAGGA GGAAACTGAG 
AGTTTGAGCT CTGGACCACT GAAACTGGAC TATAAGACAC TGGCGGCTCT GCCTACAACT 
AGTCTACAGA AAGTTAATAG ATTTTCTCCC TCCTTTTCCA CATTTGCCAA AATGGAGGCT 
GCCATGCCGG CTGGCCCCGT AGACCCCCTG GGTTTTGAGA AAGTCCATGC TTCTATGTAC 
CATGTAGAAC CTGTTGCCCT CCCTTCATCT CCAATCTGCC CAGCCGCAAG TTCAGCGGCT 
TCAAAACAGC CCCAAGCTGA CTCACGGGAC CTGGTCTTAG ATCTAGGAAT GGCTCAAGAG 
GAGGAGGAAT GTCTTGTGGA CTCCCAACCA ATCTGCTTTA GTGAAAACCC ATTTCTTGTG 
GCTAACCGCA AGGGCAAAGG CTGCCCACCA GAAGAGCGCA TTTTATCAGG GCCACCTGTG 
GGTTACGGCA AGCATGGGCA GCTGCAGCCC TGGTTGTACA GCAAGGCTTC ACCATCTGAG 
CCTTTCAGGA TTGACAGTCC TGTCCGAGAT GCTGCACACT CTCCACACAA CTCACAGCAG 
TCTCACAAAT CTTCCTCTGC GATGCTTGGA AGAGAGGTTC GATATTCAAA CATTCATTTC 
CCTTCAAATG CAAACACCAA GGATAATGCA TCAACTAAAC CTGGTGCCAT CCAGCCTCTC 
TCTCGGGTGC GACCCCCTGT GTCTCCAGCC AGTCAGGATG GCCACAGTAG TCCAAACCCC 
TTCCAGCATG GCCTCTCTCC CATCAACTCT CAGACCACTC CTCGTGCCCC ATCCCCTGAC 
ACACATGAGG AATACCCTAT CAATATTAAG CAGGTTTATA AGGCCTTCGC TGCTGTGCCA 
CACTCCCTGG CCGTACTGGA ACCCCCTCAG GATCTTTATT CTCCACGGAA CAATGTCAGT 
GCAAAACAGC CATCACCTGA GTTTCAGCCT GAGCTGAATA ATGAAGTGTT TATGGATGAG 
TGTGATGGTG AGGGCAGTGG TCCTCGTCCC TCCCTCTTAT CTGACCGCAG CGAGTACAGA 
AGCCTGGCAG CTGTGCCACG CCTGTCTCGC CCTTCAGTGG AGCCAAAGAC CCCTTCGCCT 
CTGGGTAGGC ACAGTCCAGA ACAGCGCTCC TTCAAAGACA GACAAAAGTA TTTCGAGATC 
GATGTGAAGC AACAGACTCC AGACAAACCC AAACCTCGCA TCTCACTGGT GGGAGAAGAT 
GACCTGAAGA AAATGAAGGA GGAAGAAGCA AAGCGAATTG AACAGAGGGC GCGAGAGTTC 
ATGTTGGATG AAGATGAGGA GGAGGAAGAA GAGGATCTTG CCAAGCAAGT GGCACATATG 
AAGGCTCACG GAAAGGTTGT TCTTGATGGT GTGGAGTACA AGGTAGAGAG CTTGGGTTCC 
CCGACTTCAC GGCAGTGTGC CACACCACCA AACTATAGTG CCACACCACC CAGCCACTGC 
GGAAGCTCAG GTCCTTCCTC TATCGACGGT AAAGGAGACT CCCAGCGTAA TTCAGTGGAA 
GACAGTTTCA GACTGGAGCA GAGACCCAAT TCTATGACCG GATTGATCCC AGTTTATCCC 
GGTGAATCTG CGGCTCCCAT CCGGACGGCC AAAGCCGAGC GCAGGCATCA AGATCGATTA 
CGCATGCAGA GCCCAGAGCT CACTGTCGCT TTGGACAAGG AGCTGTCGCC TGCTGAAAAA 
CGAGCCCTGG AGGCAGAGAA GAGAGCCATG TGGAGAGCAG CCAGGCCTTA TGGCTTAGAG 
GAGGACGTAA GGCAGTATGA GCAAGACCTG GCTAAGCGGC TCTACCAGTC CCGTGTGAGG 
GCATCACTGG GCAGCGCTGC AGCCCCTGCC TCTTCCTCCT GCTCCTCTTC CTCTTCCTGT 
CAGCCCAGGA TGAAGTCTTT AGAGCAGGAC GCTTTGAAGG CTCAGATGGT CATTGCCAAG 
TCTAAAGAGG GCAAGAAGCG AGGAACCCTG GATCAGCTGT CAGAGTCCCC ATCCCCTGCA 
CCCACACCCT CTCCAACACC AATGGAAGAT ATCAGTCCAC GGACAGTGAC ATCACCAGGC 
AGACTGTCCC TGTCAGAAAA GAAGTTTGAC TACCGACAAT TTGCTGCCAT TCCTTCTTCC 
AAACCCGTTT ACGACATTCA GTCTCCAGGT GCAGCTGATG ACGTGCACTT CATGGATGAG 
TCCTCCAGTA ACGCAGTGTC TGTTATTGAT CCAGAAGTTC CAGTGGCCGC CACGTCAGCT 
CTAGAAGAGA TGGCTCTCTA CAGTAACAAA CGTAAACTAC GCCAGGGCCG CCGCAGCCTG 
GAGGCTCCTG TGCCCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477419.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333094.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X6, mRNA.

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CloneID	ODa143213
Clone ID Related Accession (Same CDS sequence)	XM_021477419.1
Accession Version	XM_021477419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGCC TTATGGCTTA 
GAGGAGGACG TAAGGCAGTA TGAGCAAGAC CTGGCTAAGC GGCTCTACCA GTCCCGTGTG 
AGGGCATCAC TGGGCAGCGC TGCAGCCCCT GCCTCTTCCT CCTGCTCCTC TTCCTCTTCC 
TGTCAGCCCA GGATGAAGTC TTTAGAGCAG GACGCTTTGA AGGCTCAGAT GGTCATTGCC 
AAGTCTAAAG AGGGCAAGAA GCGAGGAACC CTGGATCAGC TGTCAGAGTC CCCATCCCCT 
GCACCCACAC CCTCTCCAAC ACCAATGGAA GATATCAGTC CACGGACAGT GACATCACCA 
GGCAGACTGT CTCCAGGTGC AGCTGATGAC GTGCACTTCA TGGATGAGTC CTCCAGTAAC 
GCAGTGTCTG TTATTGATCC AGAAGTTCCA GTGGCCGCCA CGTCAGCTCT AGAAGAGATG 
GCTCTCTACA GTAACAAACG TAAACTACGC CAGGGCCGCC GCAGCCTGGA GGCTCCTGTG 
CCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333094.1
CDS	1070..7138
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477419.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477419.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGCC TTATGGCTTA 
GAGGAGGACG TAAGGCAGTA TGAGCAAGAC CTGGCTAAGC GGCTCTACCA GTCCCGTGTG 
AGGGCATCAC TGGGCAGCGC TGCAGCCCCT GCCTCTTCCT CCTGCTCCTC TTCCTCTTCC 
TGTCAGCCCA GGATGAAGTC TTTAGAGCAG GACGCTTTGA AGGCTCAGAT GGTCATTGCC 
AAGTCTAAAG AGGGCAAGAA GCGAGGAACC CTGGATCAGC TGTCAGAGTC CCCATCCCCT 
GCACCCACAC CCTCTCCAAC ACCAATGGAA GATATCAGTC CACGGACAGT GACATCACCA 
GGCAGACTGT CTCCAGGTGC AGCTGATGAC GTGCACTTCA TGGATGAGTC CTCCAGTAAC 
GCAGTGTCTG TTATTGATCC AGAAGTTCCA GTGGCCGCCA CGTCAGCTCT AGAAGAGATG 
GCTCTCTACA GTAACAAACG TAAACTACGC CAGGGCCGCC GCAGCCTGGA GGCTCCTGTG 
CCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477421.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333096.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X7, mRNA.

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CloneID	ODa143214
Clone ID Related Accession (Same CDS sequence)	XM_021477421.1
Accession Version	XM_021477421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
ACTAAACCTG GTGCCATCCA GCCTCTCTCT CGGGTGCGAC CCCCTGTGTC TCCAGCCAGT 
CAGGATGGCC ACAGTAGTCC AAACCCCTTC CAGCATGGCC TCTCTCCCAT CAACTCTCAG 
ACCACTCCTC GTGCCCCATC CCCTGACACA CATGAGGAAT ACCCTATCAA TATTAAGCAG 
GTTTATAAGG CCTTCGCTGC TGTGCCACAC TCCCTGGCCG TACTGGAACC CCCTCAGGAT 
CTTTATTCTC CACGGAACAA TGTCAGTGCA AAACAGCCAT CACCTGAGTT TCAGCCTGAG 
CTGAATAATG AAGTGTTTAT GGATGAGTGT GATGGTGAGG GCAGTGGTCC TCGTCCCTCC 
CTCTTATCTG ACCGCAGCGA GTACAGAAGC CTGGCAGCTG TGCCACGCCT GTCTCGCCCT 
TCAGTGGAGC CAAAGACCCC TTCGCCTCTG GGTAGGCACA GTCCAGAACA GCGCTCCTTC 
AAAGACAGAC AAAAGTATTT CGAGATCGAT GTGAAGCAAC AGACTCCAGA CAAACCCAAA 
CCTCGCATCT CACTGGTGGG AGAAGATGAC CTGAAGAAAA TGAAGGAGGA AGAAGCAAAG 
CGAATTGAAC AGAGGGCGCG AGAGTTCATG TTGGATGAAG ATGAGGAGGA GGAAGAAGAG 
GATCTTGCCA AGCAAGTGGC ACATATGAAG GCTCACGGAA AGGTTGTTCT TGATGGTGTG 
GAGTACAAGG TAGAGAGCTT GGGTTCCCCG ACTTCACGGC AGTGTGCCAC ACCACCAAAC 
TATAGTGCCA CACCACCCAG CCACTGCGGA AGCTCAGGTC CTTCCTCTAT CGACGGTAAA 
GGAGACTCCC AGCGTAATTC AGTGGAAGAC AGTTTCAGAC TGGAGCAGAG ACCCAATTCT 
ATGACCGGAT TGATCCCAGT TTATCCCGGT GAATCTGCGG CTCCCATCCG GACGGCCAAA 
GCCGAGCGCA GGCATCAAGA TCGATTACGC ATGCAGAGCC CAGAGCTCAC TGTCGCTTTG 
GACAAGGAGC TGTCGCCTGC TGAAAAACGA GCCCTGGAGG CAGAGAAGAG AGCCATGTGG 
AGAGCAGCCA GGCCTTATGG CTTAGAGGAG GACGTAAGGC AGTATGAGCA AGACCTGGCT 
AAGCGGCTCT ACCAGTCCCG TGTGAGGGCA TCACTGGGCA GCGCTGCAGC CCCTGCCTCT 
TCCTCCTGCT CCTCTTCCTC TTCCTGTCAG CCCAGGATGA AGTCTTTAGA GCAGGACGCT 
TTGAAGGCTC AGATGGTCAT TGCCAAGTCT AAAGAGGGCA AGAAGCGAGG AACCCTGGAT 
CAGCTGTCAG AGTCCCCATC CCCTGCACCC ACACCCTCTC CAACACCAAT GGAAGATATC 
AGTCCACGGA CAGTGACATC ACCAGGCAGA CTGTCCCTGT CAGAAAAGAA GTTTGACTAC 
CGACAATTTG CTGCCATTCC TTCTTCCAAA CCCGTTTACG ACATTCAGTC TCCAGGTGCA 
GCTGATGACG TGCACTTCAT GGATGAGTCC TCCAGTAACG CAGTGTCTGT TATTGATCCA 
GAAGTTCCAG TGGCCGCCAC GTCAGCTCTA GAAGAGATGG CTCTCTACAG TAACAAACGT 
AAACTACGCC AGGGCCGCCG CAGCCTGGAG GCTCCTGTGC CCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333096.1
CDS	1071..7118
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477421.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477421.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
ACTAAACCTG GTGCCATCCA GCCTCTCTCT CGGGTGCGAC CCCCTGTGTC TCCAGCCAGT 
CAGGATGGCC ACAGTAGTCC AAACCCCTTC CAGCATGGCC TCTCTCCCAT CAACTCTCAG 
ACCACTCCTC GTGCCCCATC CCCTGACACA CATGAGGAAT ACCCTATCAA TATTAAGCAG 
GTTTATAAGG CCTTCGCTGC TGTGCCACAC TCCCTGGCCG TACTGGAACC CCCTCAGGAT 
CTTTATTCTC CACGGAACAA TGTCAGTGCA AAACAGCCAT CACCTGAGTT TCAGCCTGAG 
CTGAATAATG AAGTGTTTAT GGATGAGTGT GATGGTGAGG GCAGTGGTCC TCGTCCCTCC 
CTCTTATCTG ACCGCAGCGA GTACAGAAGC CTGGCAGCTG TGCCACGCCT GTCTCGCCCT 
TCAGTGGAGC CAAAGACCCC TTCGCCTCTG GGTAGGCACA GTCCAGAACA GCGCTCCTTC 
AAAGACAGAC AAAAGTATTT CGAGATCGAT GTGAAGCAAC AGACTCCAGA CAAACCCAAA 
CCTCGCATCT CACTGGTGGG AGAAGATGAC CTGAAGAAAA TGAAGGAGGA AGAAGCAAAG 
CGAATTGAAC AGAGGGCGCG AGAGTTCATG TTGGATGAAG ATGAGGAGGA GGAAGAAGAG 
GATCTTGCCA AGCAAGTGGC ACATATGAAG GCTCACGGAA AGGTTGTTCT TGATGGTGTG 
GAGTACAAGG TAGAGAGCTT GGGTTCCCCG ACTTCACGGC AGTGTGCCAC ACCACCAAAC 
TATAGTGCCA CACCACCCAG CCACTGCGGA AGCTCAGGTC CTTCCTCTAT CGACGGTAAA 
GGAGACTCCC AGCGTAATTC AGTGGAAGAC AGTTTCAGAC TGGAGCAGAG ACCCAATTCT 
ATGACCGGAT TGATCCCAGT TTATCCCGGT GAATCTGCGG CTCCCATCCG GACGGCCAAA 
GCCGAGCGCA GGCATCAAGA TCGATTACGC ATGCAGAGCC CAGAGCTCAC TGTCGCTTTG 
GACAAGGAGC TGTCGCCTGC TGAAAAACGA GCCCTGGAGG CAGAGAAGAG AGCCATGTGG 
AGAGCAGCCA GGCCTTATGG CTTAGAGGAG GACGTAAGGC AGTATGAGCA AGACCTGGCT 
AAGCGGCTCT ACCAGTCCCG TGTGAGGGCA TCACTGGGCA GCGCTGCAGC CCCTGCCTCT 
TCCTCCTGCT CCTCTTCCTC TTCCTGTCAG CCCAGGATGA AGTCTTTAGA GCAGGACGCT 
TTGAAGGCTC AGATGGTCAT TGCCAAGTCT AAAGAGGGCA AGAAGCGAGG AACCCTGGAT 
CAGCTGTCAG AGTCCCCATC CCCTGCACCC ACACCCTCTC CAACACCAAT GGAAGATATC 
AGTCCACGGA CAGTGACATC ACCAGGCAGA CTGTCCCTGT CAGAAAAGAA GTTTGACTAC 
CGACAATTTG CTGCCATTCC TTCTTCCAAA CCCGTTTACG ACATTCAGTC TCCAGGTGCA 
GCTGATGACG TGCACTTCAT GGATGAGTCC TCCAGTAACG CAGTGTCTGT TATTGATCCA 
GAAGTTCCAG TGGCCGCCAC GTCAGCTCTA GAAGAGATGG CTCTCTACAG TAACAAACGT 
AAACTACGCC AGGGCCGCCG CAGCCTGGAG GCTCCTGTGC CCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477422.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333097.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X8, mRNA.

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CloneID	ODa143215
Clone ID Related Accession (Same CDS sequence)	XM_021477422.1
Accession Version	XM_021477422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGCC TTATGGCTTA 
GAGGAGGACG TAAGGCAGTA TGAGCAAGAC CTGGCTAAGC GGCTCTACCA GTCCCGTGTG 
AGGGCATCAC TGGGCAGCGC TGCAGCCCCT GCCTCTTCCT CCTGCTCCTC TTCCTCTTCC 
TGTCAGCCCA GGATGAAGTC TTTAGAGCAG GACGCTTTGA AGGCTCAGAT GGTCATTGCC 
AAGTCTAAAG AGGGCAAGAA GCGAGGAACC CTGGATCAGC TGTCAGAGTC CCCATCCCCT 
GCACCCACAC CCTCTCCAAC ACCAATGGAA GATATCAGTC CACGGACAGT GACATCACCA 
GGCAGACTGT CCCTGTCAGA AAAGAAGTTT GACTACCGAC AATTTGCTGC CATTCCTTCT 
TCCAAACCCG TTTACGACAT TCAGTCTCCA GGTGCAGCTG ATGACGTGCA CTTCATGGAT 
GAGTCCTCCA GTAACGCAGG CGACTTCCTT GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333097.1
CDS	1071..7106
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTPRAPSPDTHEEYPINIKQVYKAFAAVPHSLAVLEPPQDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAGDFLG

Target ORF information:

RefSeq Version	XM_021477422.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477422.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTCCTCGTGC CCCATCCCCT 
GACACACATG AGGAATACCC TATCAATATT AAGCAGGTTT ATAAGGCCTT CGCTGCTGTG 
CCACACTCCC TGGCCGTACT GGAACCCCCT CAGGATCTTT ATTCTCCACG GAACAATGTC 
AGTGCAAAAC AGCCATCACC TGAGTTTCAG CCTGAGCTGA ATAATGAAGT GTTTATGGAT 
GAGTGTGATG GTGAGGGCAG TGGTCCTCGT CCCTCCCTCT TATCTGACCG CAGCGAGTAC 
AGAAGCCTGG CAGCTGTGCC ACGCCTGTCT CGCCCTTCAG TGGAGCCAAA GACCCCTTCG 
CCTCTGGGTA GGCACAGTCC AGAACAGCGC TCCTTCAAAG ACAGACAAAA GTATTTCGAG 
ATCGATGTGA AGCAACAGAC TCCAGACAAA CCCAAACCTC GCATCTCACT GGTGGGAGAA 
GATGACCTGA AGAAAATGAA GGAGGAAGAA GCAAAGCGAA TTGAACAGAG GGCGCGAGAG 
TTCATGTTGG ATGAAGATGA GGAGGAGGAA GAAGAGGATC TTGCCAAGCA AGTGGCACAT 
ATGAAGGCTC ACGGAAAGGT TGTTCTTGAT GGTGTGGAGT ACAAGGTAGA GAGCTTGGGT 
TCCCCGACTT CACGGCAGTG TGCCACACCA CCAAACTATA GTGCCACACC ACCCAGCCAC 
TGCGGAAGCT CAGGTCCTTC CTCTATCGAC GGTAAAGGAG ACTCCCAGCG TAATTCAGTG 
GAAGACAGTT TCAGACTGGA GCAGAGACCC AATTCTATGA CCGGATTGAT CCCAGTTTAT 
CCCGGTGAAT CTGCGGCTCC CATCCGGACG GCCAAAGCCG AGCGCAGGCA TCAAGATCGA 
TTACGCATGC AGAGCCCAGA GCTCACTGTC GCTTTGGACA AGGAGCTGTC GCCTGCTGAA 
AAACGAGCCC TGGAGGCAGA GAAGAGAGCC ATGTGGAGAG CAGCCAGGCC TTATGGCTTA 
GAGGAGGACG TAAGGCAGTA TGAGCAAGAC CTGGCTAAGC GGCTCTACCA GTCCCGTGTG 
AGGGCATCAC TGGGCAGCGC TGCAGCCCCT GCCTCTTCCT CCTGCTCCTC TTCCTCTTCC 
TGTCAGCCCA GGATGAAGTC TTTAGAGCAG GACGCTTTGA AGGCTCAGAT GGTCATTGCC 
AAGTCTAAAG AGGGCAAGAA GCGAGGAACC CTGGATCAGC TGTCAGAGTC CCCATCCCCT 
GCACCCACAC CCTCTCCAAC ACCAATGGAA GATATCAGTC CACGGACAGT GACATCACCA 
GGCAGACTGT CCCTGTCAGA AAAGAAGTTT GACTACCGAC AATTTGCTGC CATTCCTTCT 
TCCAAACCCG TTTACGACAT TCAGTCTCCA GGTGCAGCTG ATGACGTGCA CTTCATGGAT 
GAGTCCTCCA GTAACGCAGG CGACTTCCTT GGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scrib ( XM_021477423.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333098.1 Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X9, mRNA.

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CloneID	ODa143216
Clone ID Related Accession (Same CDS sequence)	XM_021477423.1
Accession Version	XM_021477423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein scribble homolog isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTGATCTTTA TTCTCCACGG 
AACAATGTCA GTGCAAAACA GCCATCACCT GAGTTTCAGC CTGAGCTGAA TAATGAAGTG 
TTTATGGATG AGTGTGATGG TGAGGGCAGT GGTCCTCGTC CCTCCCTCTT ATCTGACCGC 
AGCGAGTACA GAAGCCTGGC AGCTGTGCCA CGCCTGTCTC GCCCTTCAGT GGAGCCAAAG 
ACCCCTTCGC CTCTGGGTAG GCACAGTCCA GAACAGCGCT CCTTCAAAGA CAGACAAAAG 
TATTTCGAGA TCGATGTGAA GCAACAGACT CCAGACAAAC CCAAACCTCG CATCTCACTG 
GTGGGAGAAG ATGACCTGAA GAAAATGAAG GAGGAAGAAG CAAAGCGAAT TGAACAGAGG 
GCGCGAGAGT TCATGTTGGA TGAAGATGAG GAGGAGGAAG AAGAGGATCT TGCCAAGCAA 
GTGGCACATA TGAAGGCTCA CGGAAAGGTT GTTCTTGATG GTGTGGAGTA CAAGGTAGAG 
AGCTTGGGTT CCCCGACTTC ACGGCAGTGT GCCACACCAC CAAACTATAG TGCCACACCA 
CCCAGCCACT GCGGAAGCTC AGGTCCTTCC TCTATCGACG GTAAAGGAGA CTCCCAGCGT 
AATTCAGTGG AAGACAGTTT CAGACTGGAG CAGAGACCCA ATTCTATGAC CGGATTGATC 
CCAGTTTATC CCGGTGAATC TGCGGCTCCC ATCCGGACGG CCAAAGCCGA GCGCAGGCAT 
CAAGATCGAT TACGCATGCA GAGCCCAGAG CTCACTGTCG CTTTGGACAA GGAGCTGTCG 
CCTGCTGAAA AACGAGCCCT GGAGGCAGAG AAGAGAGCCA TGTGGAGAGC AGCCAGGCCT 
TATGGCTTAG AGGAGGACGT AAGGCAGTAT GAGCAAGACC TGGCTAAGCG GCTCTACCAG 
TCCCGTGTGA GGGCATCACT GGGCAGCGCT GCAGCCCCTG CCTCTTCCTC CTGCTCCTCT 
TCCTCTTCCT GTCAGCCCAG GATGAAGTCT TTAGAGCAGG ACGCTTTGAA GGCTCAGATG 
GTCATTGCCA AGTCTAAAGA GGGCAAGAAG CGAGGAACCC TGGATCAGCT GTCAGAGTCC 
CCATCCCCTG CACCCACACC CTCTCCAACA CCAATGGAAG ATATCAGTCC ACGGACAGTG 
ACATCACCAG GCAGACTGTC CCTGTCAGAA AAGAAGTTTG ACTACCGACA ATTTGCTGCC 
ATTCCTTCTT CCAAACCCGT TTACGACATT CAGTCTCCAG GTGCAGCTGA TGACGTGCAC 
TTCATGGATG AGTCCTCCAG TAACGCAGTG TCTGTTATTG ATCCAGAAGT TCCAGTGGCC 
GCCACGTCAG CTCTAGAAGA GATGGCTCTC TACAGTAACA AACGTAAACT ACGCCAGGGC 
CGCCGCAGCC TGGAGGCTCC TGTGCCCACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333098.1
CDS	1070..7102
Translation	MLKCIPLWRCNRHVESVDKRHCSLTAVPDEIYRYNRSLEELLLDANQLRELPKPFFRLHNLRKLGLSDNEIQKLPPDVANFTQLVELDISRNDISEIPENIKFCQSLEIADFSGNPLTRLPDGFTQLRGLAHLSLNDVSLQSLPNDIGNLSNLVTLELRENLLKSLPSSLSFLVKLEQLDLGSNVLEVLPDTLGALPNLRELWLDRNQLSSLPPELGNLRQLVCLDVSENRLSELPTEISGLIALTDLLLSENLLEILPDSIGSLKKLSILKVNQNRLVHLTDSIGECENLTELMLTENLLQSLPRSLGKLKKLTNLNVDRNRLSSVPAELGGCVSLNVLSLRDNRLGKLPPELANATELHVLDVAGNRLQNLPFALANLNLKAMWLAENQSQPMLKFQTEDDEQTGEKVLTCYLLPQQPSSSLENLLERSVDEAWPTDTNLNRVSVIQFRDDSKHEEEDDETAADKRQGLQRRATPHPSELKVMKNVIEARRNEAYTARPDEDLESPDAEEKRLSGLSNQSHDSQASSSTTSATSHEDKQGINSTGVELNDGQVQEEEDLDEMEVEYTEPTVHFAEEPIIRGGDEDDDYDNDDDDAERSDEVSQTPSFPAEKQRLIRKDTPHYKKHFKITKLPKPETVAALLQGFSHDGLSPTAHTPENERDGEDDEEEEEDEDEEDDLHTPFQHRLDAAELEDIRMSQMNSSLQPVKGVSFDQVNNLLIEPARIEEEELTLSILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVKVGDKLLEVNGVDLHGAEHHTAVEALRNSGAAVVMTVLRERMVEPENAITTTPLRPEDDYFPRERRSSGLPFLLDPDCPAVSTGPAQRLATCLIRNDKGLGFSIAGGKGSTLYRVGDTGIFISRIAEGGAAHRDNILQVGDRVISINGVDMTEARHDQAVALLTGTSPTITLVVDREQSSVGGASPRTRPHSPPPPEPSDSPEQEDGGDEHLGNHLNCPMEDEYPIEEVTLIKAGGPLGLSIVGGSDHASHPFGINEPGVFISKVIPNGLASQSGLRVGDRILEVNSIDLRHATHQEAVRALLSNKQEIRMLVRRDPSPPGMQEIVIHKQPGEKLGISIRGGAKGHAGNPFDPTDEGIFISKVSSNGAAARDGRLRVGMRILEVGNNSLLGMTHTEAVRVLRASGDSLVMLICDGFDPKSASTIEASPGVIANPFAAGIVRKNSMESISSVDRDLSPEEIDIMQKEVEMVRETSQWEREEMEKVERMRLEREEATRLLEEETESLSSGPLKLDYKTLAALPTTSLQKVNRFSPSFSTFAKMEAAMPAGPVDPLGFEKVHASMYHVEPVALPSSPICPAASSAASKQPQADSRDLVLDLGMAQEEEECLVDSQPICFSENPFLVANRKGKGCPPEERILSGPPVGYGKHGQLQPWLYSKASPSEPFRIDSPVRDAAHSPHNSQQSHKSSSAMLGREVRYSNIHFPSNANTKDNASTKPGAIQPLSRVRPPVSPASQDGHSSPNPFQHGLSPINSQTTDLYSPRNNVSAKQPSPEFQPELNNEVFMDECDGEGSGPRPSLLSDRSEYRSLAAVPRLSRPSVEPKTPSPLGRHSPEQRSFKDRQKYFEIDVKQQTPDKPKPRISLVGEDDLKKMKEEEAKRIEQRAREFMLDEDEEEEEEDLAKQVAHMKAHGKVVLDGVEYKVESLGSPTSRQCATPPNYSATPPSHCGSSGPSSIDGKGDSQRNSVEDSFRLEQRPNSMTGLIPVYPGESAAPIRTAKAERRHQDRLRMQSPELTVALDKELSPAEKRALEAEKRAMWRAARPYGLEEDVRQYEQDLAKRLYQSRVRASLGSAAAPASSSCSSSSSCQPRMKSLEQDALKAQMVIAKSKEGKKRGTLDQLSESPSPAPTPSPTPMEDISPRTVTSPGRLSLSEKKFDYRQFAAIPSSKPVYDIQSPGAADDVHFMDESSSNAVSVIDPEVPVAATSALEEMALYSNKRKLRQGRRSLEAPVPT

Target ORF information:

RefSeq Version	XM_021477423.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio scribbled planar cell polarity protein (scrib), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477423.1

ORF Insert Sequence:

ATGCTGAAGT GTATCCCGCT GTGGCGGTGC AACCGTCACG TCGAGTCTGT GGATAAACGA 
CACTGCTCGC TCACTGCGGT GCCCGATGAG ATCTACCGCT ACAACCGCAG TCTGGAGGAG 
CTGCTCCTGG ACGCCAACCA GCTGAGAGAG CTGCCTAAGC CTTTCTTCAG ACTGCACAAT 
TTGCGAAAGC TGGGCCTCAG TGACAACGAG ATTCAAAAAT TACCACCAGA CGTTGCCAAC 
TTCACGCAGC TAGTGGAGCT TGACATCTCT AGAAATGATA TTTCTGAGAT TCCTGAAAAT 
ATAAAATTCT GCCAGTCTTT GGAGATTGCA GACTTCAGCG GAAACCCCTT GACCAGGTTG 
CCTGATGGCT TCACGCAGCT CCGAGGACTG GCTCACCTAT CACTCAATGA CGTGTCGCTG 
CAGTCTCTAC CCAATGACAT AGGAAACTTG TCAAACCTGG TGACTCTAGA GCTGAGGGAG 
AATCTACTGA AATCCTTGCC CTCCTCTCTT TCCTTCCTGG TAAAACTGGA ACAGCTGGAT 
CTGGGCAGCA ATGTACTGGA AGTTTTGCCT GACACACTTG GGGCGCTGCC CAATCTTCGT 
GAGCTGTGGT TGGACAGAAA CCAGCTCTCC TCACTACCTC CTGAGCTGGG GAACCTGCGG 
CAGCTGGTTT GCTTGGACGT GTCAGAAAAC CGTCTATCAG AGCTTCCCAC AGAGATCAGC 
GGCCTTATCG CCCTCACGGA CCTGCTGCTC TCAGAAAACC TCCTGGAGAT CTTGCCGGAC 
AGCATCGGCT CACTTAAGAA GTTGTCCATT TTGAAAGTGA ACCAGAACCG CCTTGTGCAT 
CTGACTGACT CTATTGGGGA GTGTGAGAAC CTGACAGAGC TCATGTTGAC CGAGAACCTA 
CTGCAGTCTC TTCCGCGCTC ACTTGGGAAG CTGAAGAAGT TGACCAACTT GAATGTGGAC 
CGAAACCGTT TGAGCAGCGT GCCTGCAGAG CTGGGCGGAT GTGTTAGCCT GAATGTGCTC 
TCTCTCAGAG ATAACCGTTT GGGAAAACTA CCTCCAGAGC TCGCCAATGC CACTGAATTA 
CATGTGCTTG ATGTGGCAGG CAACAGGTTG CAGAACTTGC CTTTCGCATT GGCCAACCTG 
AACCTGAAAG CTATGTGGCT GGCAGAGAAC CAGTCTCAAC CCATGCTCAA GTTCCAGACA 
GAGGATGATG AACAGACTGG AGAGAAAGTT CTCACCTGCT ACCTGCTGCC CCAACAACCC 
TCATCTAGCC TCGAGAATCT GTTGGAGAGG AGCGTTGATG AAGCGTGGCC CACAGACACT 
AATTTGAACA GAGTATCAGT TATTCAGTTC CGGGACGACT CCAAACATGA AGAGGAAGAT 
GATGAGACTG CGGCAGACAA GAGGCAGGGT CTTCAGAGGA GAGCCACACC ACACCCTAGT 
GAGTTAAAGG TGATGAAGAA TGTAATTGAA GCAAGAAGAA ATGAGGCTTA TACAGCCAGA 
CCAGATGAAG ATCTAGAATC CCCAGATGCT GAGGAGAAAC GGTTAAGTGG TCTGTCCAAT 
CAGAGTCATG ATTCTCAGGC ATCCAGTAGC ACCACCTCAG CCACTTCCCA TGAGGACAAG 
CAGGGTATAA ACAGTACAGG GGTGGAGCTT AATGATGGAC AGGTTCAAGA AGAGGAGGAT 
CTGGATGAGA TGGAGGTGGA GTATACTGAG CCTACTGTGC ACTTTGCTGA AGAGCCCATC 
ATCCGTGGTG GTGATGAGGA CGACGATTAT GATAATGACG ATGATGACGC TGAAAGGAGT 
GACGAGGTGT CTCAAACGCC ATCTTTTCCT GCCGAGAAAC AGCGGCTCAT ACGTAAAGAC 
ACACCACACT ACAAAAAGCA CTTCAAGATC ACCAAGCTGC CCAAACCAGA GACAGTCGCC 
GCCCTGCTGC AAGGCTTCAG TCATGATGGC CTGTCACCTA CTGCTCACAC CCCTGAGAAC 
GAGAGGGATG GAGAGGATGA CGAGGAAGAG GAGGAGGATG AAGACGAGGA GGACGATCTC 
CATACTCCTT TCCAGCACAG ACTAGATGCA GCAGAGCTAG AAGACATCAG AATGAGTCAG 
ATGAACTCGT CCCTGCAGCC AGTGAAGGGG GTGTCATTTG ATCAAGTCAA TAATCTGCTG 
ATTGAACCTG CTCGAATTGA GGAGGAAGAG CTGACCTTGA GCATCCTGAG GCAGACTGGT 
GGATTAGGCA TCAGCATAGC AGGAGGGAAG GGATCTACTC CCTATAAAGG TGACGATGAG 
GGTATTTTCA TCTCTAGGGT GTCAGAAGAA GGCCCTGCGG CTCGTGCTGG TGTCAAAGTT 
GGAGACAAAC TTCTTGAGGT GAATGGAGTA GACCTGCACG GAGCGGAGCA TCATACAGCT 
GTTGAAGCTT TGCGCAATTC AGGAGCAGCC GTGGTCATGA CTGTTTTGCG GGAGCGCATG 
GTGGAACCAG AAAACGCCAT CACCACCACA CCGTTGCGGC CAGAAGATGA TTATTTCCCC 
CGGGAGAGAC GCTCCAGTGG GCTTCCTTTC CTCCTGGATC CTGATTGTCC CGCTGTTAGC 
ACTGGACCCG CACAGCGACT AGCCACTTGT CTGATCCGCA ATGATAAAGG CTTAGGCTTC 
AGCATTGCTG GTGGGAAAGG GTCAACGCTG TACAGAGTCG GAGACACGGG CATCTTCATC 
TCTCGTATTG CTGAAGGAGG AGCAGCTCAC AGAGACAACA TCCTGCAAGT GGGAGACAGG 
GTCATATCAA TCAATGGTGT AGACATGACA GAGGCCAGGC ATGACCAGGC AGTAGCGCTG 
CTTACCGGCA CCTCCCCCAC CATCACCCTG GTGGTGGATC GGGAACAAAG CAGTGTAGGC 
GGAGCCTCCC CTCGAACCCG ACCACATTCG CCCCCACCTC CAGAGCCCTC TGACTCCCCT 
GAGCAAGAGG ACGGTGGAGA CGAGCACCTT GGAAACCATC TCAACTGCCC CATGGAAGAT 
GAGTACCCTA TTGAGGAAGT GACGCTGATC AAAGCAGGTG GACCCCTGGG TTTGAGCATT 
GTAGGGGGCA GCGACCATGC CAGCCATCCA TTTGGAATTA ATGAACCTGG AGTGTTCATC 
TCAAAGGTCA TCCCCAATGG CCTGGCCTCC CAGAGTGGAC TTCGCGTGGG TGATCGTATC 
TTGGAGGTGA ACAGCATTGA TCTGCGTCAT GCCACACACC AGGAGGCTGT TAGAGCCTTG 
CTTTCCAACA AACAGGAGAT CCGTATGCTT GTGCGCAGAG ATCCTTCTCC CCCTGGCATG 
CAGGAAATTG TCATTCACAA GCAACCTGGA GAAAAGCTGG GAATAAGCAT TAGGGGCGGA 
GCTAAAGGTC ATGCAGGAAA CCCCTTTGAC CCCACAGATG AGGGCATCTT TATTTCCAAG 
GTGAGCTCCA ACGGTGCTGC TGCACGAGAT GGCCGACTGC GGGTGGGTAT GCGTATTCTT 
GAGGTGGGCA ACAACAGTCT GCTGGGTATG ACACATACAG AAGCAGTGAG GGTCTTGCGT 
GCTTCTGGAG ATTCCCTGGT CATGCTTATT TGTGATGGCT TTGACCCAAA AAGTGCTTCA 
ACCATAGAGG CATCTCCTGG TGTTATTGCG AATCCGTTTG CGGCAGGTAT TGTTCGTAAA 
AACAGTATGG AAAGCATCTC ATCAGTCGAC AGAGACCTGA GTCCAGAAGA GATTGACATC 
ATGCAGAAGG AGGTAGAAAT GGTAAGAGAG ACGTCCCAGT GGGAACGAGA GGAGATGGAA 
AAAGTGGAGC GCATGCGCTT GGAGCGCGAG GAGGCAACTC GCTTGCTGGA GGAGGAAACT 
GAGAGTTTGA GCTCTGGACC ACTGAAACTG GACTATAAGA CACTGGCGGC TCTGCCTACA 
ACTAGTCTAC AGAAAGTTAA TAGATTTTCT CCCTCCTTTT CCACATTTGC CAAAATGGAG 
GCTGCCATGC CGGCTGGCCC CGTAGACCCC CTGGGTTTTG AGAAAGTCCA TGCTTCTATG 
TACCATGTAG AACCTGTTGC CCTCCCTTCA TCTCCAATCT GCCCAGCCGC AAGTTCAGCG 
GCTTCAAAAC AGCCCCAAGC TGACTCACGG GACCTGGTCT TAGATCTAGG AATGGCTCAA 
GAGGAGGAGG AATGTCTTGT GGACTCCCAA CCAATCTGCT TTAGTGAAAA CCCATTTCTT 
GTGGCTAACC GCAAGGGCAA AGGCTGCCCA CCAGAAGAGC GCATTTTATC AGGGCCACCT 
GTGGGTTACG GCAAGCATGG GCAGCTGCAG CCCTGGTTGT ACAGCAAGGC TTCACCATCT 
GAGCCTTTCA GGATTGACAG TCCTGTCCGA GATGCTGCAC ACTCTCCACA CAACTCACAG 
CAGTCTCACA AATCTTCCTC TGCGATGCTT GGAAGAGAGG TTCGATATTC AAACATTCAT 
TTCCCTTCAA ATGCAAACAC CAAGGATAAT GCATCAACTA AACCTGGTGC CATCCAGCCT 
CTCTCTCGGG TGCGACCCCC TGTGTCTCCA GCCAGTCAGG ATGGCCACAG TAGTCCAAAC 
CCCTTCCAGC ATGGCCTCTC TCCCATCAAC TCTCAGACCA CTGATCTTTA TTCTCCACGG 
AACAATGTCA GTGCAAAACA GCCATCACCT GAGTTTCAGC CTGAGCTGAA TAATGAAGTG 
TTTATGGATG AGTGTGATGG TGAGGGCAGT GGTCCTCGTC CCTCCCTCTT ATCTGACCGC 
AGCGAGTACA GAAGCCTGGC AGCTGTGCCA CGCCTGTCTC GCCCTTCAGT GGAGCCAAAG 
ACCCCTTCGC CTCTGGGTAG GCACAGTCCA GAACAGCGCT CCTTCAAAGA CAGACAAAAG 
TATTTCGAGA TCGATGTGAA GCAACAGACT CCAGACAAAC CCAAACCTCG CATCTCACTG 
GTGGGAGAAG ATGACCTGAA GAAAATGAAG GAGGAAGAAG CAAAGCGAAT TGAACAGAGG 
GCGCGAGAGT TCATGTTGGA TGAAGATGAG GAGGAGGAAG AAGAGGATCT TGCCAAGCAA 
GTGGCACATA TGAAGGCTCA CGGAAAGGTT GTTCTTGATG GTGTGGAGTA CAAGGTAGAG 
AGCTTGGGTT CCCCGACTTC ACGGCAGTGT GCCACACCAC CAAACTATAG TGCCACACCA 
CCCAGCCACT GCGGAAGCTC AGGTCCTTCC TCTATCGACG GTAAAGGAGA CTCCCAGCGT 
AATTCAGTGG AAGACAGTTT CAGACTGGAG CAGAGACCCA ATTCTATGAC CGGATTGATC 
CCAGTTTATC CCGGTGAATC TGCGGCTCCC ATCCGGACGG CCAAAGCCGA GCGCAGGCAT 
CAAGATCGAT TACGCATGCA GAGCCCAGAG CTCACTGTCG CTTTGGACAA GGAGCTGTCG 
CCTGCTGAAA AACGAGCCCT GGAGGCAGAG AAGAGAGCCA TGTGGAGAGC AGCCAGGCCT 
TATGGCTTAG AGGAGGACGT AAGGCAGTAT GAGCAAGACC TGGCTAAGCG GCTCTACCAG 
TCCCGTGTGA GGGCATCACT GGGCAGCGCT GCAGCCCCTG CCTCTTCCTC CTGCTCCTCT 
TCCTCTTCCT GTCAGCCCAG GATGAAGTCT TTAGAGCAGG ACGCTTTGAA GGCTCAGATG 
GTCATTGCCA AGTCTAAAGA GGGCAAGAAG CGAGGAACCC TGGATCAGCT GTCAGAGTCC 
CCATCCCCTG CACCCACACC CTCTCCAACA CCAATGGAAG ATATCAGTCC ACGGACAGTG 
ACATCACCAG GCAGACTGTC CCTGTCAGAA AAGAAGTTTG ACTACCGACA ATTTGCTGCC 
ATTCCTTCTT CCAAACCCGT TTACGACATT CAGTCTCCAG GTGCAGCTGA TGACGTGCAC 
TTCATGGATG AGTCCTCCAG TAACGCAGTG TCTGTTATTG ATCCAGAAGT TCCAGTGGCC 
GCCACGTCAG CTCTAGAAGA GATGGCTCTC TACAGTAACA AACGTAAACT ACGCCAGGGC 
CGCCGCAGCC TGGAGGCTCC TGTGCCCACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Planar cell polarity genes Frizzled3a, Vangl2, and Scribble are required for spinal commissural axon guidance.
BMC neuroscience17(1)83(2016 12)
Sun SD,Purdy AM,Walsh GS

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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scrib cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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