toporsa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	toporsa
Entrez Gene ID	324197
Full Name	topoisomerase I binding, arginine/serine-rich a
Synonyms	si:ch211-145b13.4,topors,wu:fc21e07
General protein information	Preferred Names topoisomerase I binding, arginine/serine-rich a Names E3 ubiquitin-protein ligase Topors
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305555.1	NP_001292484.1	E3 ubiquitin-protein ligase Topors
XM_017357742.1	XP_017213231.1	E3 ubiquitin-protein ligase Topors isoform X1
XM_017357742.2	XP_017213231.1	E3 ubiquitin-protein ligase Topors isoform X1
XM_021479343.1	XP_021335018.1	E3 ubiquitin-protein ligase Topors isoform X1
XM_021479283.1	XP_021334958.1	E3 ubiquitin-protein ligase Topors isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

TOPORS, implicated in retinal degeneration, is a cilia-centrosomal protein.
Chakarova CF, Khanna H, Shah AZ, Patil SB, Sedmak T, Murga-Zamalloa CA, Papaioannou MG, Nagel-Wolfrum K, Lopez I, Munro P, Cheetham M, Koenekoop RK, Rios RM, Matter K, Wolfrum U, Swaroop A, Bhattacharya SS
Human molecular genetics20(5)975-87(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following toporsa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the toporsa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa05782	NM_001305555.1 Latest version!	Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa05782	XM_021479343.1 Latest version!	Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa05782	XM_017357742.2 Latest version!	Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa05782	XM_021479283.1 Latest version!	Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa05782	XM_017357742.1	Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

toporsa ( NM_001305555.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001292484.1 Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), mRNA.

Price & Availability↑

CloneID	ODa05782
Clone ID Related Accession (Same CDS sequence)	NM_001305555.1 , XM_017357742.1 , XM_017357742.2 , XM_021479343.1 , XM_021479283.1
Accession Version	NM_001305555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase Topors
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH593328.1 and BX323448.7. On Mar 18, 2015 this sequence version replaced XM_682803.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC090477.1, EH593328.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCAC CACAGATAAA GGAGCGGCCA TCAGGTGTTG TGTTAAACAC AATCTCAAAA 
GGAGCATCTC CAGAATCAAA ATGCCCAATC TGCCTGGATC ACTTTAAAAA CATATCATAT 
CTTGATGTGT GCCTTCACAA GTTCTGCTTC TGCTGCATCC ATGAATGGTC CAAGAACAAA 
GCTGAATGCC CTTTATGCAA ACAGCCATTT AATTCATTCT ATCACACTAT TAAGTCTGAA 
GATGACTACA AGAGGTTTGA CCTGCGACCA ACTGAAAATG GCTCATTTGG AAACATGGCA 
GGGCAAAGAT TCAGGTATCG CACCACGCTT ACAGGTGACC GCAGACCAGT ACAGAGGCGA 
ACGTCTCCTC CTCCCGACCA TGGGATCATA TTTGAGGGTC TGAGAGGATC TCTTCCACAA 
CGGCACAACA GAGATCTACA TAGCATGATC AGGAGGCTGA CAGTAAGGCA GAGAAGAGAG 
AGTGAGGGTA GGTCTTTGCG AAGTCTTCAA GATCAAGAAG TGGTTAAGTT CAGACGAGCC 
TTGTACAGAA GAGGTGTGCA GGTCCAAAGC GTGCAGGATG GTGGGCGTAC GAGAGAGACC 
TCAGCAGAGT TCTTCAAAAG AAATCCTGCT TGCCTCCACA GGCTTGTACC TTGGCTGAGA 
CGAGAACTGA CTGTTCTGTA CGGTGCTCAT GGTTCTCTGG TCAACATTGT GCAGCACATC 
ATTATGACTC TGATCACTCG ACATAACTTG GACGACCAGG CTGTTCAACA TGAGCTTCGG 
CCCTTCTTAC TGTCCCACAC AGAGCACTTC TTACATGAGT TTCTCAGCTT TGCTCGATCG 
CCGTTCAATA TGGAGGCATA TGACCAACGT GCTGTTTATG ACTTGCCAAG ACCTTCAGGA 
GAAAGTAGCA GTTCGGAAAA CTCTGTGATC GCCATCTCTG AGGATGAGAG CGAATCTTTA 
GTGTCAGGAG CCCAGGATTT TGCCACTCCT AGGGTGACCT CAAGCCAAAC AGCATGGGAT 
GATGAGACTC CAGGACCATC CTACTCTTCA GAGCGTTCTC AGGTGTTACC CGTCCACGTG 
AGGGACTCAG ACTCTGATAG TAGTGTAGGG GAAGCAGTAA TGCCTATTGC TGCAGTACGA 
CATCAGGACC ATCCAGCAAA TCCTTCTACT ATTCAACCAG AAGAGGATCA TTCCTCTAGC 
AATGATGAGG ACTGTGTTAT TATTGGCTAT GTTAAGCCCG TGGCAGAGAG GACACCAGAA 
CTGGTCCAGC TTTCTTCTGA TTCTGAAAAT TCCGAGACTG AGCAGAATGC AAGTCCACGA 
AACTCTCAAG CTCCCCAACA CATTCGTTTT ATTTCAGATT CAGATGCATC ACCCACTGCC 
TCACAGAGAC CCTTGAGGGG GCCAGCAAGT TCTTCACGCT CATTTGAAGA CACCTCCATA 
CAACTTAGAA AAAACAGTAA ATATGACAAT ACTTTTTCAG GATCTAGAGA AGGATCCTCA 
CAAAGCAAAA GGATACACTC TTCGTCTCAT AGAAAGAAGC AACCATTGTG TAAAGAGAGC 
AAGAATAAGC AGAGAGGGGA AAAGGAAACA AGTCGTCACT CTTCATCCTA TTGGCATTCT 
TATAGCTATT ACAGTCATGA CAACAGCAGA AAAGCTTATA CAGAAACAAG CTCATCTTAT 
ACAAGCTACT ACAGAAGTGT AAATGATCAG TTTGGTTCTC GATCACACGG CAAAAGAAGA 
TGTAGGGACC TTCTGGACAG AAGTCGCTCA AGGAGTAATT CAAGAATAAG CCAGTCGAAT 
CGGCGTAATA GGAGTCGCTC AAGGAGTAAT TCAAGAATAA GCCGGTCCAA TCAACGGAAT 
AGAAGTCACT CAAGGAGTAA TTCTGGAAAA AGCCAATCCA ATCGGCGTAA TAGAAGTTGT 
TCAAGGAGTT ATTCAAGAAC AAGCCCATTT AATGAACGGA ATAGAAGACA CTCAAGGAGT 
AATTCTAGAA AAAGCCAATC CAATCGGCAT AATAGAAGTT GTTCAAGGAG TTATTCAAGA 
ACAAGCCCAT TTAATGAACG GAATAGAAGA CACTCAAGGA GTAATTCTAG AAAAAGCCAA 
TCCAATCGGC ATAATAGAAG TTGTTCAAGG AGTTATTCAA GAACAAGCCC ATTTAATCAA 
CGGAATAGAA GACACTCAAG GAGTAATTCT AGAATAAGCC AGTCCAGTCG GCAGAACAGA 
AGTCTCTCAA GGAGTAATTC TAGAAAAGGC TATTCAAATC GGCGGAACAG AAATCATTCT 
AGGAGTAGTT CTAGAGTAAG CCAGTCCAGT CAGCGGAACA GAAGTCACTC AAGGAGTAAT 
TTTAGAATCA AGTCTAGCTC CTACAAAAGT CAAGATGAAA AATATCAAAG AAGCCTTTCT 
AGATCCCTTT ACTCCAATAA TGATGTCAGA AGTCGCTCAA GAAGCAGTTC CAGAATAAAC 
ACCTCTAGCC GCCAGGACCG AAGACGCTCA AGGAGCAGTT CTAGAATGAA TTCCAGCTCC 
TCACGCCAGA CATCATGCCA CGATAAATAT GGTAAAAAAA GGATGTACAA AACAAAGCAT 
CATGAGAAAC CCTCAAGGCA GAGTTCTAGT AAATCGGTTG GCGATTCTGA TGAAGTAGGT 
GGAAAAAAGA AAAAAAAGAA GCATACTAAA AGGAGGAAAA CTAGAAGTCC AAGCATAGAT 
GACAGATCTG AGGGCCACAG TCAGAGACAT CACAAGAGGA AGAAGAAACA CAAGAAGAAG 
AGCAAGAGAC ATAACAGTAA GGAGAGGACG AGAAAGCTCA GCCCTGTTCT CATTACAATT 
ACTACTGACA GTGATGGTGA AAGTGCTGAC CCCAGTGAAC CCTCAACCAG CAGTGTTTGT 
GCCATTAGAT CTACTGTCTT AATAGCAAAC ATGGCTACAG AAGCTTTCCT AGACAATGAG 
CATCAGTCAA CAGCTGGAAC TGAAAATAGT TCTGTGGGTG ATGCAGGAAA TTACCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292484.1
CDS	263..3262
Translation	MASPQIKERPSGVVLNTISKGASPESKCPICLDHFKNISYLDVCLHKFCFCCIHEWSKNKAECPLCKQPFNSFYHTIKSEDDYKRFDLRPTENGSFGNMAGQRFRYRTTLTGDRRPVQRRTSPPPDHGIIFEGLRGSLPQRHNRDLHSMIRRLTVRQRRESEGRSLRSLQDQEVVKFRRALYRRGVQVQSVQDGGRTRETSAEFFKRNPACLHRLVPWLRRELTVLYGAHGSLVNIVQHIIMTLITRHNLDDQAVQHELRPFLLSHTEHFLHEFLSFARSPFNMEAYDQRAVYDLPRPSGESSSSENSVIAISEDESESLVSGAQDFATPRVTSSQTAWDDETPGPSYSSERSQVLPVHVRDSDSDSSVGEAVMPIAAVRHQDHPANPSTIQPEEDHSSSNDEDCVIIGYVKPVAERTPELVQLSSDSENSETEQNASPRNSQAPQHIRFISDSDASPTASQRPLRGPASSSRSFEDTSIQLRKNSKYDNTFSGSREGSSQSKRIHSSSHRKKQPLCKESKNKQRGEKETSRHSSSYWHSYSYYSHDNSRKAYTETSSSYTSYYRSVNDQFGSRSHGKRRCRDLLDRSRSRSNSRISQSNRRNRSRSRSNSRISRSNQRNRSHSRSNSGKSQSNRRNRSCSRSYSRTSPFNERNRRHSRSNSRKSQSNRHNRSCSRSYSRTSPFNERNRRHSRSNSRKSQSNRHNRSCSRSYSRTSPFNQRNRRHSRSNSRISQSSRQNRSLSRSNSRKGYSNRRNRNHSRSSSRVSQSSQRNRSHSRSNFRIKSSSYKSQDEKYQRSLSRSLYSNNDVRSRSRSSSRINTSSRQDRRRSRSSSRMNSSSSRQTSCHDKYGKKRMYKTKHHEKPSRQSSSKSVGDSDEVGGKKKKKKHTKRRKTRSPSIDDRSEGHSQRHHKRKKKHKKKSKRHNSKERTRKLSPVLITITTDSDGESADPSEPSTSSVCAIRSTVLIANMATEAFLDNEHQSTAGTENSSVGDAGNYP

Target ORF information:

RefSeq Version	NM_001305555.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305555.1

ORF Insert Sequence:

ATGGCATCAC CACAGATAAA GGAGCGGCCA TCAGGTGTTG TGTTAAACAC AATCTCAAAA 
GGAGCATCTC CAGAATCAAA ATGCCCAATC TGCCTGGATC ACTTTAAAAA CATATCATAT 
CTTGATGTGT GCCTTCACAA GTTCTGCTTC TGCTGCATCC ATGAATGGTC CAAGAACAAA 
GCTGAATGCC CTTTATGCAA ACAGCCATTT AATTCATTCT ATCACACTAT TAAGTCTGAA 
GATGACTACA AGAGGTTTGA CCTGCGACCA ACTGAAAATG GCTCATTTGG AAACATGGCA 
GGGCAAAGAT TCAGGTATCG CACCACGCTT ACAGGTGACC GCAGACCAGT ACAGAGGCGA 
ACGTCTCCTC CTCCCGACCA TGGGATCATA TTTGAGGGTC TGAGAGGATC TCTTCCACAA 
CGGCACAACA GAGATCTACA TAGCATGATC AGGAGGCTGA CAGTAAGGCA GAGAAGAGAG 
AGTGAGGGTA GGTCTTTGCG AAGTCTTCAA GATCAAGAAG TGGTTAAGTT CAGACGAGCC 
TTGTACAGAA GAGGTGTGCA GGTCCAAAGC GTGCAGGATG GTGGGCGTAC GAGAGAGACC 
TCAGCAGAGT TCTTCAAAAG AAATCCTGCT TGCCTCCACA GGCTTGTACC TTGGCTGAGA 
CGAGAACTGA CTGTTCTGTA CGGTGCTCAT GGTTCTCTGG TCAACATTGT GCAGCACATC 
ATTATGACTC TGATCACTCG ACATAACTTG GACGACCAGG CTGTTCAACA TGAGCTTCGG 
CCCTTCTTAC TGTCCCACAC AGAGCACTTC TTACATGAGT TTCTCAGCTT TGCTCGATCG 
CCGTTCAATA TGGAGGCATA TGACCAACGT GCTGTTTATG ACTTGCCAAG ACCTTCAGGA 
GAAAGTAGCA GTTCGGAAAA CTCTGTGATC GCCATCTCTG AGGATGAGAG CGAATCTTTA 
GTGTCAGGAG CCCAGGATTT TGCCACTCCT AGGGTGACCT CAAGCCAAAC AGCATGGGAT 
GATGAGACTC CAGGACCATC CTACTCTTCA GAGCGTTCTC AGGTGTTACC CGTCCACGTG 
AGGGACTCAG ACTCTGATAG TAGTGTAGGG GAAGCAGTAA TGCCTATTGC TGCAGTACGA 
CATCAGGACC ATCCAGCAAA TCCTTCTACT ATTCAACCAG AAGAGGATCA TTCCTCTAGC 
AATGATGAGG ACTGTGTTAT TATTGGCTAT GTTAAGCCCG TGGCAGAGAG GACACCAGAA 
CTGGTCCAGC TTTCTTCTGA TTCTGAAAAT TCCGAGACTG AGCAGAATGC AAGTCCACGA 
AACTCTCAAG CTCCCCAACA CATTCGTTTT ATTTCAGATT CAGATGCATC ACCCACTGCC 
TCACAGAGAC CCTTGAGGGG GCCAGCAAGT TCTTCACGCT CATTTGAAGA CACCTCCATA 
CAACTTAGAA AAAACAGTAA ATATGACAAT ACTTTTTCAG GATCTAGAGA AGGATCCTCA 
CAAAGCAAAA GGATACACTC TTCGTCTCAT AGAAAGAAGC AACCATTGTG TAAAGAGAGC 
AAGAATAAGC AGAGAGGGGA AAAGGAAACA AGTCGTCACT CTTCATCCTA TTGGCATTCT 
TATAGCTATT ACAGTCATGA CAACAGCAGA AAAGCTTATA CAGAAACAAG CTCATCTTAT 
ACAAGCTACT ACAGAAGTGT AAATGATCAG TTTGGTTCTC GATCACACGG CAAAAGAAGA 
TGTAGGGACC TTCTGGACAG AAGTCGCTCA AGGAGTAATT CAAGAATAAG CCAGTCGAAT 
CGGCGTAATA GGAGTCGCTC AAGGAGTAAT TCAAGAATAA GCCGGTCCAA TCAACGGAAT 
AGAAGTCACT CAAGGAGTAA TTCTGGAAAA AGCCAATCCA ATCGGCGTAA TAGAAGTTGT 
TCAAGGAGTT ATTCAAGAAC AAGCCCATTT AATGAACGGA ATAGAAGACA CTCAAGGAGT 
AATTCTAGAA AAAGCCAATC CAATCGGCAT AATAGAAGTT GTTCAAGGAG TTATTCAAGA 
ACAAGCCCAT TTAATGAACG GAATAGAAGA CACTCAAGGA GTAATTCTAG AAAAAGCCAA 
TCCAATCGGC ATAATAGAAG TTGTTCAAGG AGTTATTCAA GAACAAGCCC ATTTAATCAA 
CGGAATAGAA GACACTCAAG GAGTAATTCT AGAATAAGCC AGTCCAGTCG GCAGAACAGA 
AGTCTCTCAA GGAGTAATTC TAGAAAAGGC TATTCAAATC GGCGGAACAG AAATCATTCT 
AGGAGTAGTT CTAGAGTAAG CCAGTCCAGT CAGCGGAACA GAAGTCACTC AAGGAGTAAT 
TTTAGAATCA AGTCTAGCTC CTACAAAAGT CAAGATGAAA AATATCAAAG AAGCCTTTCT 
AGATCCCTTT ACTCCAATAA TGATGTCAGA AGTCGCTCAA GAAGCAGTTC CAGAATAAAC 
ACCTCTAGCC GCCAGGACCG AAGACGCTCA AGGAGCAGTT CTAGAATGAA TTCCAGCTCC 
TCACGCCAGA CATCATGCCA CGATAAATAT GGTAAAAAAA GGATGTACAA AACAAAGCAT 
CATGAGAAAC CCTCAAGGCA GAGTTCTAGT AAATCGGTTG GCGATTCTGA TGAAGTAGGT 
GGAAAAAAGA AAAAAAAGAA GCATACTAAA AGGAGGAAAA CTAGAAGTCC AAGCATAGAT 
GACAGATCTG AGGGCCACAG TCAGAGACAT CACAAGAGGA AGAAGAAACA CAAGAAGAAG 
AGCAAGAGAC ATAACAGTAA GGAGAGGACG AGAAAGCTCA GCCCTGTTCT CATTACAATT 
ACTACTGACA GTGATGGTGA AAGTGCTGAC CCCAGTGAAC CCTCAACCAG CAGTGTTTGT 
GCCATTAGAT CTACTGTCTT AATAGCAAAC ATGGCTACAG AAGCTTTCCT AGACAATGAG 
CATCAGTCAA CAGCTGGAAC TGAAAATAGT TCTGTGGGTG ATGCAGGAAA TTACCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

toporsa ( XM_017357742.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213231.1 Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05782
Clone ID Related Accession (Same CDS sequence)	NM_001305555.1 , XM_017357742.1 , XM_017357742.2 , XM_021479343.1 , XM_021479283.1
Accession Version	XM_017357742.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase Topors isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCAC CACAGATAAA GGAGCGGCCA TCAGGTGTTG TGTTAAACAC AATCTCAAAA 
GGAGCATCTC CAGAATCAAA ATGCCCAATC TGCCTGGATC ACTTTAAAAA CATATCATAT 
CTTGATGTGT GCCTTCACAA GTTCTGCTTC TGCTGCATCC ATGAATGGTC CAAGAACAAA 
GCTGAATGCC CTTTATGCAA ACAGCCATTT AATTCATTCT ATCACACTAT TAAGTCTGAA 
GATGACTACA AGAGGTTTGA CCTGCGACCA ACTGAAAATG GCTCATTTGG AAACATGGCA 
GGGCAAAGAT TCAGGTATCG CACCACGCTT ACAGGTGACC GCAGACCAGT ACAGAGGCGA 
ACGTCTCCTC CTCCCGACCA TGGGATCATA TTTGAGGGTC TGAGAGGATC TCTTCCACAA 
CGGCACAACA GAGATCTACA TAGCATGATC AGGAGGCTGA CAGTAAGGCA GAGAAGAGAG 
AGTGAGGGTA GGTCTTTGCG AAGTCTTCAA GATCAAGAAG TGGTTAAGTT CAGACGAGCC 
TTGTACAGAA GAGGTGTGCA GGTCCAAAGC GTGCAGGATG GTGGGCGTAC GAGAGAGACC 
TCAGCAGAGT TCTTCAAAAG AAATCCTGCT TGCCTCCACA GGCTTGTACC TTGGCTGAGA 
CGAGAACTGA CTGTTCTGTA CGGTGCTCAT GGTTCTCTGG TCAACATTGT GCAGCACATC 
ATTATGACTC TGATCACTCG ACATAACTTG GACGACCAGG CTGTTCAACA TGAGCTTCGG 
CCCTTCTTAC TGTCCCACAC AGAGCACTTC TTACATGAGT TTCTCAGCTT TGCTCGATCG 
CCGTTCAATA TGGAGGCATA TGACCAACGT GCTGTTTATG ACTTGCCAAG ACCTTCAGGA 
GAAAGTAGCA GTTCGGAAAA CTCTGTGATC GCCATCTCTG AGGATGAGAG CGAATCTTTA 
GTGTCAGGAG CCCAGGATTT TGCCACTCCT AGGGTGACCT CAAGCCAAAC AGCATGGGAT 
GATGAGACTC CAGGACCATC CTACTCTTCA GAGCGTTCTC AGGTGTTACC CGTCCACGTG 
AGGGACTCAG ACTCTGATAG TAGTGTAGGG GAAGCAGTAA TGCCTATTGC TGCAGTACGA 
CATCAGGACC ATCCAGCAAA TCCTTCTACT ATTCAACCAG AAGAGGATCA TTCCTCTAGC 
AATGATGAGG ACTGTGTTAT TATTGGCTAT GTTAAGCCCG TGGCAGAGAG GACACCAGAA 
CTGGTCCAGC TTTCTTCTGA TTCTGAAAAT TCCGAGACTG AGCAGAATGC AAGTCCACGA 
AACTCTCAAG CTCCCCAACA CATTCGTTTT ATTTCAGATT CAGATGCATC ACCCACTGCC 
TCACAGAGAC CCTTGAGGGG GCCAGCAAGT TCTTCACGCT CATTTGAAGA CACCTCCATA 
CAACTTAGAA AAAACAGTAA ATATGACAAT ACTTTTTCAG GATCTAGAGA AGGATCCTCA 
CAAAGCAAAA GGATACACTC TTCGTCTCAT AGAAAGAAGC AACCATTGTG TAAAGAGAGC 
AAGAATAAGC AGAGAGGGGA AAAGGAAACA AGTCGTCACT CTTCATCCTA TTGGCATTCT 
TATAGCTATT ACAGTCATGA CAACAGCAGA AAAGCTTATA CAGAAACAAG CTCATCTTAT 
ACAAGCTACT ACAGAAGTGT AAATGATCAG TTTGGTTCTC GATCACACGG CAAAAGAAGA 
TGTAGGGACC TTCTGGACAG AAGTCGCTCA AGGAGTAATT CAAGAATAAG CCAGTCGAAT 
CGGCGTAATA GGAGTCGCTC AAGGAGTAAT TCAAGAATAA GCCGGTCCAA TCAACGGAAT 
AGAAGTCACT CAAGGAGTAA TTCTGGAAAA AGCCAATCCA ATCGGCGTAA TAGAAGTTGT 
TCAAGGAGTT ATTCAAGAAC AAGCCCATTT AATGAACGGA ATAGAAGACA CTCAAGGAGT 
AATTCTAGAA AAAGCCAATC CAATCGGCAT AATAGAAGTT GTTCAAGGAG TTATTCAAGA 
ACAAGCCCAT TTAATGAACG GAATAGAAGA CACTCAAGGA GTAATTCTAG AAAAAGCCAA 
TCCAATCGGC ATAATAGAAG TTGTTCAAGG AGTTATTCAA GAACAAGCCC ATTTAATCAA 
CGGAATAGAA GACACTCAAG GAGTAATTCT AGAATAAGCC AGTCCAGTCG GCAGAACAGA 
AGTCTCTCAA GGAGTAATTC TAGAAAAGGC TATTCAAATC GGCGGAACAG AAATCATTCT 
AGGAGTAGTT CTAGAGTAAG CCAGTCCAGT CAGCGGAACA GAAGTCACTC AAGGAGTAAT 
TTTAGAATCA AGTCTAGCTC CTACAAAAGT CAAGATGAAA AATATCAAAG AAGCCTTTCT 
AGATCCCTTT ACTCCAATAA TGATGTCAGA AGTCGCTCAA GAAGCAGTTC CAGAATAAAC 
ACCTCTAGCC GCCAGGACCG AAGACGCTCA AGGAGCAGTT CTAGAATGAA TTCCAGCTCC 
TCACGCCAGA CATCATGCCA CGATAAATAT GGTAAAAAAA GGATGTACAA AACAAAGCAT 
CATGAGAAAC CCTCAAGGCA GAGTTCTAGT AAATCGGTTG GCGATTCTGA TGAAGTAGGT 
GGAAAAAAGA AAAAAAAGAA GCATACTAAA AGGAGGAAAA CTAGAAGTCC AAGCATAGAT 
GACAGATCTG AGGGCCACAG TCAGAGACAT CACAAGAGGA AGAAGAAACA CAAGAAGAAG 
AGCAAGAGAC ATAACAGTAA GGAGAGGACG AGAAAGCTCA GCCCTGTTCT CATTACAATT 
ACTACTGACA GTGATGGTGA AAGTGCTGAC CCCAGTGAAC CCTCAACCAG CAGTGTTTGT 
GCCATTAGAT CTACTGTCTT AATAGCAAAC ATGGCTACAG AAGCTTTCCT AGACAATGAG 
CATCAGTCAA CAGCTGGAAC TGAAAATAGT TCTGTGGGTG ATGCAGGAAA TTACCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213231.1
CDS	378..3377
Translation	MASPQIKERPSGVVLNTISKGASPESKCPICLDHFKNISYLDVCLHKFCFCCIHEWSKNKAECPLCKQPFNSFYHTIKSEDDYKRFDLRPTENGSFGNMAGQRFRYRTTLTGDRRPVQRRTSPPPDHGIIFEGLRGSLPQRHNRDLHSMIRRLTVRQRRESEGRSLRSLQDQEVVKFRRALYRRGVQVQSVQDGGRTRETSAEFFKRNPACLHRLVPWLRRELTVLYGAHGSLVNIVQHIIMTLITRHNLDDQAVQHELRPFLLSHTEHFLHEFLSFARSPFNMEAYDQRAVYDLPRPSGESSSSENSVIAISEDESESLVSGAQDFATPRVTSSQTAWDDETPGPSYSSERSQVLPVHVRDSDSDSSVGEAVMPIAAVRHQDHPANPSTIQPEEDHSSSNDEDCVIIGYVKPVAERTPELVQLSSDSENSETEQNASPRNSQAPQHIRFISDSDASPTASQRPLRGPASSSRSFEDTSIQLRKNSKYDNTFSGSREGSSQSKRIHSSSHRKKQPLCKESKNKQRGEKETSRHSSSYWHSYSYYSHDNSRKAYTETSSSYTSYYRSVNDQFGSRSHGKRRCRDLLDRSRSRSNSRISQSNRRNRSRSRSNSRISRSNQRNRSHSRSNSGKSQSNRRNRSCSRSYSRTSPFNERNRRHSRSNSRKSQSNRHNRSCSRSYSRTSPFNERNRRHSRSNSRKSQSNRHNRSCSRSYSRTSPFNQRNRRHSRSNSRISQSSRQNRSLSRSNSRKGYSNRRNRNHSRSSSRVSQSSQRNRSHSRSNFRIKSSSYKSQDEKYQRSLSRSLYSNNDVRSRSRSSSRINTSSRQDRRRSRSSSRMNSSSSRQTSCHDKYGKKRMYKTKHHEKPSRQSSSKSVGDSDEVGGKKKKKKHTKRRKTRSPSIDDRSEGHSQRHHKRKKKHKKKSKRHNSKERTRKLSPVLITITTDSDGESADPSEPSTSSVCAIRSTVLIANMATEAFLDNEHQSTAGTENSSVGDAGNYP

Target ORF information:

RefSeq Version	XM_017357742.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357742.1

ORF Insert Sequence:

ATGGCATCAC CACAGATAAA GGAGCGGCCA TCAGGTGTTG TGTTAAACAC AATCTCAAAA 
GGAGCATCTC CAGAATCAAA ATGCCCAATC TGCCTGGATC ACTTTAAAAA CATATCATAT 
CTTGATGTGT GCCTTCACAA GTTCTGCTTC TGCTGCATCC ATGAATGGTC CAAGAACAAA 
GCTGAATGCC CTTTATGCAA ACAGCCATTT AATTCATTCT ATCACACTAT TAAGTCTGAA 
GATGACTACA AGAGGTTTGA CCTGCGACCA ACTGAAAATG GCTCATTTGG AAACATGGCA 
GGGCAAAGAT TCAGGTATCG CACCACGCTT ACAGGTGACC GCAGACCAGT ACAGAGGCGA 
ACGTCTCCTC CTCCCGACCA TGGGATCATA TTTGAGGGTC TGAGAGGATC TCTTCCACAA 
CGGCACAACA GAGATCTACA TAGCATGATC AGGAGGCTGA CAGTAAGGCA GAGAAGAGAG 
AGTGAGGGTA GGTCTTTGCG AAGTCTTCAA GATCAAGAAG TGGTTAAGTT CAGACGAGCC 
TTGTACAGAA GAGGTGTGCA GGTCCAAAGC GTGCAGGATG GTGGGCGTAC GAGAGAGACC 
TCAGCAGAGT TCTTCAAAAG AAATCCTGCT TGCCTCCACA GGCTTGTACC TTGGCTGAGA 
CGAGAACTGA CTGTTCTGTA CGGTGCTCAT GGTTCTCTGG TCAACATTGT GCAGCACATC 
ATTATGACTC TGATCACTCG ACATAACTTG GACGACCAGG CTGTTCAACA TGAGCTTCGG 
CCCTTCTTAC TGTCCCACAC AGAGCACTTC TTACATGAGT TTCTCAGCTT TGCTCGATCG 
CCGTTCAATA TGGAGGCATA TGACCAACGT GCTGTTTATG ACTTGCCAAG ACCTTCAGGA 
GAAAGTAGCA GTTCGGAAAA CTCTGTGATC GCCATCTCTG AGGATGAGAG CGAATCTTTA 
GTGTCAGGAG CCCAGGATTT TGCCACTCCT AGGGTGACCT CAAGCCAAAC AGCATGGGAT 
GATGAGACTC CAGGACCATC CTACTCTTCA GAGCGTTCTC AGGTGTTACC CGTCCACGTG 
AGGGACTCAG ACTCTGATAG TAGTGTAGGG GAAGCAGTAA TGCCTATTGC TGCAGTACGA 
CATCAGGACC ATCCAGCAAA TCCTTCTACT ATTCAACCAG AAGAGGATCA TTCCTCTAGC 
AATGATGAGG ACTGTGTTAT TATTGGCTAT GTTAAGCCCG TGGCAGAGAG GACACCAGAA 
CTGGTCCAGC TTTCTTCTGA TTCTGAAAAT TCCGAGACTG AGCAGAATGC AAGTCCACGA 
AACTCTCAAG CTCCCCAACA CATTCGTTTT ATTTCAGATT CAGATGCATC ACCCACTGCC 
TCACAGAGAC CCTTGAGGGG GCCAGCAAGT TCTTCACGCT CATTTGAAGA CACCTCCATA 
CAACTTAGAA AAAACAGTAA ATATGACAAT ACTTTTTCAG GATCTAGAGA AGGATCCTCA 
CAAAGCAAAA GGATACACTC TTCGTCTCAT AGAAAGAAGC AACCATTGTG TAAAGAGAGC 
AAGAATAAGC AGAGAGGGGA AAAGGAAACA AGTCGTCACT CTTCATCCTA TTGGCATTCT 
TATAGCTATT ACAGTCATGA CAACAGCAGA AAAGCTTATA CAGAAACAAG CTCATCTTAT 
ACAAGCTACT ACAGAAGTGT AAATGATCAG TTTGGTTCTC GATCACACGG CAAAAGAAGA 
TGTAGGGACC TTCTGGACAG AAGTCGCTCA AGGAGTAATT CAAGAATAAG CCAGTCGAAT 
CGGCGTAATA GGAGTCGCTC AAGGAGTAAT TCAAGAATAA GCCGGTCCAA TCAACGGAAT 
AGAAGTCACT CAAGGAGTAA TTCTGGAAAA AGCCAATCCA ATCGGCGTAA TAGAAGTTGT 
TCAAGGAGTT ATTCAAGAAC AAGCCCATTT AATGAACGGA ATAGAAGACA CTCAAGGAGT 
AATTCTAGAA AAAGCCAATC CAATCGGCAT AATAGAAGTT GTTCAAGGAG TTATTCAAGA 
ACAAGCCCAT TTAATGAACG GAATAGAAGA CACTCAAGGA GTAATTCTAG AAAAAGCCAA 
TCCAATCGGC ATAATAGAAG TTGTTCAAGG AGTTATTCAA GAACAAGCCC ATTTAATCAA 
CGGAATAGAA GACACTCAAG GAGTAATTCT AGAATAAGCC AGTCCAGTCG GCAGAACAGA 
AGTCTCTCAA GGAGTAATTC TAGAAAAGGC TATTCAAATC GGCGGAACAG AAATCATTCT 
AGGAGTAGTT CTAGAGTAAG CCAGTCCAGT CAGCGGAACA GAAGTCACTC AAGGAGTAAT 
TTTAGAATCA AGTCTAGCTC CTACAAAAGT CAAGATGAAA AATATCAAAG AAGCCTTTCT 
AGATCCCTTT ACTCCAATAA TGATGTCAGA AGTCGCTCAA GAAGCAGTTC CAGAATAAAC 
ACCTCTAGCC GCCAGGACCG AAGACGCTCA AGGAGCAGTT CTAGAATGAA TTCCAGCTCC 
TCACGCCAGA CATCATGCCA CGATAAATAT GGTAAAAAAA GGATGTACAA AACAAAGCAT 
CATGAGAAAC CCTCAAGGCA GAGTTCTAGT AAATCGGTTG GCGATTCTGA TGAAGTAGGT 
GGAAAAAAGA AAAAAAAGAA GCATACTAAA AGGAGGAAAA CTAGAAGTCC AAGCATAGAT 
GACAGATCTG AGGGCCACAG TCAGAGACAT CACAAGAGGA AGAAGAAACA CAAGAAGAAG 
AGCAAGAGAC ATAACAGTAA GGAGAGGACG AGAAAGCTCA GCCCTGTTCT CATTACAATT 
ACTACTGACA GTGATGGTGA AAGTGCTGAC CCCAGTGAAC CCTCAACCAG CAGTGTTTGT 
GCCATTAGAT CTACTGTCTT AATAGCAAAC ATGGCTACAG AAGCTTTCCT AGACAATGAG 
CATCAGTCAA CAGCTGGAAC TGAAAATAGT TCTGTGGGTG ATGCAGGAAA TTACCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

toporsa ( XM_017357742.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213231.1 Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa05782
Clone ID Related Accession (Same CDS sequence)	NM_001305555.1 , XM_017357742.1 , XM_017357742.2 , XM_021479343.1 , XM_021479283.1
Accession Version	XM_017357742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase Topors isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCAC CACAGATAAA GGAGCGGCCA TCAGGTGTTG TGTTAAACAC AATCTCAAAA 
GGAGCATCTC CAGAATCAAA ATGCCCAATC TGCCTGGATC ACTTTAAAAA CATATCATAT 
CTTGATGTGT GCCTTCACAA GTTCTGCTTC TGCTGCATCC ATGAATGGTC CAAGAACAAA 
GCTGAATGCC CTTTATGCAA ACAGCCATTT AATTCATTCT ATCACACTAT TAAGTCTGAA 
GATGACTACA AGAGGTTTGA CCTGCGACCA ACTGAAAATG GCTCATTTGG AAACATGGCA 
GGGCAAAGAT TCAGGTATCG CACCACGCTT ACAGGTGACC GCAGACCAGT ACAGAGGCGA 
ACGTCTCCTC CTCCCGACCA TGGGATCATA TTTGAGGGTC TGAGAGGATC TCTTCCACAA 
CGGCACAACA GAGATCTACA TAGCATGATC AGGAGGCTGA CAGTAAGGCA GAGAAGAGAG 
AGTGAGGGTA GGTCTTTGCG AAGTCTTCAA GATCAAGAAG TGGTTAAGTT CAGACGAGCC 
TTGTACAGAA GAGGTGTGCA GGTCCAAAGC GTGCAGGATG GTGGGCGTAC GAGAGAGACC 
TCAGCAGAGT TCTTCAAAAG AAATCCTGCT TGCCTCCACA GGCTTGTACC TTGGCTGAGA 
CGAGAACTGA CTGTTCTGTA CGGTGCTCAT GGTTCTCTGG TCAACATTGT GCAGCACATC 
ATTATGACTC TGATCACTCG ACATAACTTG GACGACCAGG CTGTTCAACA TGAGCTTCGG 
CCCTTCTTAC TGTCCCACAC AGAGCACTTC TTACATGAGT TTCTCAGCTT TGCTCGATCG 
CCGTTCAATA TGGAGGCATA TGACCAACGT GCTGTTTATG ACTTGCCAAG ACCTTCAGGA 
GAAAGTAGCA GTTCGGAAAA CTCTGTGATC GCCATCTCTG AGGATGAGAG CGAATCTTTA 
GTGTCAGGAG CCCAGGATTT TGCCACTCCT AGGGTGACCT CAAGCCAAAC AGCATGGGAT 
GATGAGACTC CAGGACCATC CTACTCTTCA GAGCGTTCTC AGGTGTTACC CGTCCACGTG 
AGGGACTCAG ACTCTGATAG TAGTGTAGGG GAAGCAGTAA TGCCTATTGC TGCAGTACGA 
CATCAGGACC ATCCAGCAAA TCCTTCTACT ATTCAACCAG AAGAGGATCA TTCCTCTAGC 
AATGATGAGG ACTGTGTTAT TATTGGCTAT GTTAAGCCCG TGGCAGAGAG GACACCAGAA 
CTGGTCCAGC TTTCTTCTGA TTCTGAAAAT TCCGAGACTG AGCAGAATGC AAGTCCACGA 
AACTCTCAAG CTCCCCAACA CATTCGTTTT ATTTCAGATT CAGATGCATC ACCCACTGCC 
TCACAGAGAC CCTTGAGGGG GCCAGCAAGT TCTTCACGCT CATTTGAAGA CACCTCCATA 
CAACTTAGAA AAAACAGTAA ATATGACAAT ACTTTTTCAG GATCTAGAGA AGGATCCTCA 
CAAAGCAAAA GGATACACTC TTCGTCTCAT AGAAAGAAGC AACCATTGTG TAAAGAGAGC 
AAGAATAAGC AGAGAGGGGA AAAGGAAACA AGTCGTCACT CTTCATCCTA TTGGCATTCT 
TATAGCTATT ACAGTCATGA CAACAGCAGA AAAGCTTATA CAGAAACAAG CTCATCTTAT 
ACAAGCTACT ACAGAAGTGT AAATGATCAG TTTGGTTCTC GATCACACGG CAAAAGAAGA 
TGTAGGGACC TTCTGGACAG AAGTCGCTCA AGGAGTAATT CAAGAATAAG CCAGTCGAAT 
CGGCGTAATA GGAGTCGCTC AAGGAGTAAT TCAAGAATAA GCCGGTCCAA TCAACGGAAT 
AGAAGTCACT CAAGGAGTAA TTCTGGAAAA AGCCAATCCA ATCGGCGTAA TAGAAGTTGT 
TCAAGGAGTT ATTCAAGAAC AAGCCCATTT AATGAACGGA ATAGAAGACA CTCAAGGAGT 
AATTCTAGAA AAAGCCAATC CAATCGGCAT AATAGAAGTT GTTCAAGGAG TTATTCAAGA 
ACAAGCCCAT TTAATGAACG GAATAGAAGA CACTCAAGGA GTAATTCTAG AAAAAGCCAA 
TCCAATCGGC ATAATAGAAG TTGTTCAAGG AGTTATTCAA GAACAAGCCC ATTTAATCAA 
CGGAATAGAA GACACTCAAG GAGTAATTCT AGAATAAGCC AGTCCAGTCG GCAGAACAGA 
AGTCTCTCAA GGAGTAATTC TAGAAAAGGC TATTCAAATC GGCGGAACAG AAATCATTCT 
AGGAGTAGTT CTAGAGTAAG CCAGTCCAGT CAGCGGAACA GAAGTCACTC AAGGAGTAAT 
TTTAGAATCA AGTCTAGCTC CTACAAAAGT CAAGATGAAA AATATCAAAG AAGCCTTTCT 
AGATCCCTTT ACTCCAATAA TGATGTCAGA AGTCGCTCAA GAAGCAGTTC CAGAATAAAC 
ACCTCTAGCC GCCAGGACCG AAGACGCTCA AGGAGCAGTT CTAGAATGAA TTCCAGCTCC 
TCACGCCAGA CATCATGCCA CGATAAATAT GGTAAAAAAA GGATGTACAA AACAAAGCAT 
CATGAGAAAC CCTCAAGGCA GAGTTCTAGT AAATCGGTTG GCGATTCTGA TGAAGTAGGT 
GGAAAAAAGA AAAAAAAGAA GCATACTAAA AGGAGGAAAA CTAGAAGTCC AAGCATAGAT 
GACAGATCTG AGGGCCACAG TCAGAGACAT CACAAGAGGA AGAAGAAACA CAAGAAGAAG 
AGCAAGAGAC ATAACAGTAA GGAGAGGACG AGAAAGCTCA GCCCTGTTCT CATTACAATT 
ACTACTGACA GTGATGGTGA AAGTGCTGAC CCCAGTGAAC CCTCAACCAG CAGTGTTTGT 
GCCATTAGAT CTACTGTCTT AATAGCAAAC ATGGCTACAG AAGCTTTCCT AGACAATGAG 
CATCAGTCAA CAGCTGGAAC TGAAAATAGT TCTGTGGGTG ATGCAGGAAA TTACCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213231.1
CDS	164..3163
Translation	MASPQIKERPSGVVLNTISKGASPESKCPICLDHFKNISYLDVCLHKFCFCCIHEWSKNKAECPLCKQPFNSFYHTIKSEDDYKRFDLRPTENGSFGNMAGQRFRYRTTLTGDRRPVQRRTSPPPDHGIIFEGLRGSLPQRHNRDLHSMIRRLTVRQRRESEGRSLRSLQDQEVVKFRRALYRRGVQVQSVQDGGRTRETSAEFFKRNPACLHRLVPWLRRELTVLYGAHGSLVNIVQHIIMTLITRHNLDDQAVQHELRPFLLSHTEHFLHEFLSFARSPFNMEAYDQRAVYDLPRPSGESSSSENSVIAISEDESESLVSGAQDFATPRVTSSQTAWDDETPGPSYSSERSQVLPVHVRDSDSDSSVGEAVMPIAAVRHQDHPANPSTIQPEEDHSSSNDEDCVIIGYVKPVAERTPELVQLSSDSENSETEQNASPRNSQAPQHIRFISDSDASPTASQRPLRGPASSSRSFEDTSIQLRKNSKYDNTFSGSREGSSQSKRIHSSSHRKKQPLCKESKNKQRGEKETSRHSSSYWHSYSYYSHDNSRKAYTETSSSYTSYYRSVNDQFGSRSHGKRRCRDLLDRSRSRSNSRISQSNRRNRSRSRSNSRISRSNQRNRSHSRSNSGKSQSNRRNRSCSRSYSRTSPFNERNRRHSRSNSRKSQSNRHNRSCSRSYSRTSPFNERNRRHSRSNSRKSQSNRHNRSCSRSYSRTSPFNQRNRRHSRSNSRISQSSRQNRSLSRSNSRKGYSNRRNRNHSRSSSRVSQSSQRNRSHSRSNFRIKSSSYKSQDEKYQRSLSRSLYSNNDVRSRSRSSSRINTSSRQDRRRSRSSSRMNSSSSRQTSCHDKYGKKRMYKTKHHEKPSRQSSSKSVGDSDEVGGKKKKKKHTKRRKTRSPSIDDRSEGHSQRHHKRKKKHKKKSKRHNSKERTRKLSPVLITITTDSDGESADPSEPSTSSVCAIRSTVLIANMATEAFLDNEHQSTAGTENSSVGDAGNYP

Target ORF information:

RefSeq Version	XM_017357742.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357742.2

ORF Insert Sequence:

ATGGCATCAC CACAGATAAA GGAGCGGCCA TCAGGTGTTG TGTTAAACAC AATCTCAAAA 
GGAGCATCTC CAGAATCAAA ATGCCCAATC TGCCTGGATC ACTTTAAAAA CATATCATAT 
CTTGATGTGT GCCTTCACAA GTTCTGCTTC TGCTGCATCC ATGAATGGTC CAAGAACAAA 
GCTGAATGCC CTTTATGCAA ACAGCCATTT AATTCATTCT ATCACACTAT TAAGTCTGAA 
GATGACTACA AGAGGTTTGA CCTGCGACCA ACTGAAAATG GCTCATTTGG AAACATGGCA 
GGGCAAAGAT TCAGGTATCG CACCACGCTT ACAGGTGACC GCAGACCAGT ACAGAGGCGA 
ACGTCTCCTC CTCCCGACCA TGGGATCATA TTTGAGGGTC TGAGAGGATC TCTTCCACAA 
CGGCACAACA GAGATCTACA TAGCATGATC AGGAGGCTGA CAGTAAGGCA GAGAAGAGAG 
AGTGAGGGTA GGTCTTTGCG AAGTCTTCAA GATCAAGAAG TGGTTAAGTT CAGACGAGCC 
TTGTACAGAA GAGGTGTGCA GGTCCAAAGC GTGCAGGATG GTGGGCGTAC GAGAGAGACC 
TCAGCAGAGT TCTTCAAAAG AAATCCTGCT TGCCTCCACA GGCTTGTACC TTGGCTGAGA 
CGAGAACTGA CTGTTCTGTA CGGTGCTCAT GGTTCTCTGG TCAACATTGT GCAGCACATC 
ATTATGACTC TGATCACTCG ACATAACTTG GACGACCAGG CTGTTCAACA TGAGCTTCGG 
CCCTTCTTAC TGTCCCACAC AGAGCACTTC TTACATGAGT TTCTCAGCTT TGCTCGATCG 
CCGTTCAATA TGGAGGCATA TGACCAACGT GCTGTTTATG ACTTGCCAAG ACCTTCAGGA 
GAAAGTAGCA GTTCGGAAAA CTCTGTGATC GCCATCTCTG AGGATGAGAG CGAATCTTTA 
GTGTCAGGAG CCCAGGATTT TGCCACTCCT AGGGTGACCT CAAGCCAAAC AGCATGGGAT 
GATGAGACTC CAGGACCATC CTACTCTTCA GAGCGTTCTC AGGTGTTACC CGTCCACGTG 
AGGGACTCAG ACTCTGATAG TAGTGTAGGG GAAGCAGTAA TGCCTATTGC TGCAGTACGA 
CATCAGGACC ATCCAGCAAA TCCTTCTACT ATTCAACCAG AAGAGGATCA TTCCTCTAGC 
AATGATGAGG ACTGTGTTAT TATTGGCTAT GTTAAGCCCG TGGCAGAGAG GACACCAGAA 
CTGGTCCAGC TTTCTTCTGA TTCTGAAAAT TCCGAGACTG AGCAGAATGC AAGTCCACGA 
AACTCTCAAG CTCCCCAACA CATTCGTTTT ATTTCAGATT CAGATGCATC ACCCACTGCC 
TCACAGAGAC CCTTGAGGGG GCCAGCAAGT TCTTCACGCT CATTTGAAGA CACCTCCATA 
CAACTTAGAA AAAACAGTAA ATATGACAAT ACTTTTTCAG GATCTAGAGA AGGATCCTCA 
CAAAGCAAAA GGATACACTC TTCGTCTCAT AGAAAGAAGC AACCATTGTG TAAAGAGAGC 
AAGAATAAGC AGAGAGGGGA AAAGGAAACA AGTCGTCACT CTTCATCCTA TTGGCATTCT 
TATAGCTATT ACAGTCATGA CAACAGCAGA AAAGCTTATA CAGAAACAAG CTCATCTTAT 
ACAAGCTACT ACAGAAGTGT AAATGATCAG TTTGGTTCTC GATCACACGG CAAAAGAAGA 
TGTAGGGACC TTCTGGACAG AAGTCGCTCA AGGAGTAATT CAAGAATAAG CCAGTCGAAT 
CGGCGTAATA GGAGTCGCTC AAGGAGTAAT TCAAGAATAA GCCGGTCCAA TCAACGGAAT 
AGAAGTCACT CAAGGAGTAA TTCTGGAAAA AGCCAATCCA ATCGGCGTAA TAGAAGTTGT 
TCAAGGAGTT ATTCAAGAAC AAGCCCATTT AATGAACGGA ATAGAAGACA CTCAAGGAGT 
AATTCTAGAA AAAGCCAATC CAATCGGCAT AATAGAAGTT GTTCAAGGAG TTATTCAAGA 
ACAAGCCCAT TTAATGAACG GAATAGAAGA CACTCAAGGA GTAATTCTAG AAAAAGCCAA 
TCCAATCGGC ATAATAGAAG TTGTTCAAGG AGTTATTCAA GAACAAGCCC ATTTAATCAA 
CGGAATAGAA GACACTCAAG GAGTAATTCT AGAATAAGCC AGTCCAGTCG GCAGAACAGA 
AGTCTCTCAA GGAGTAATTC TAGAAAAGGC TATTCAAATC GGCGGAACAG AAATCATTCT 
AGGAGTAGTT CTAGAGTAAG CCAGTCCAGT CAGCGGAACA GAAGTCACTC AAGGAGTAAT 
TTTAGAATCA AGTCTAGCTC CTACAAAAGT CAAGATGAAA AATATCAAAG AAGCCTTTCT 
AGATCCCTTT ACTCCAATAA TGATGTCAGA AGTCGCTCAA GAAGCAGTTC CAGAATAAAC 
ACCTCTAGCC GCCAGGACCG AAGACGCTCA AGGAGCAGTT CTAGAATGAA TTCCAGCTCC 
TCACGCCAGA CATCATGCCA CGATAAATAT GGTAAAAAAA GGATGTACAA AACAAAGCAT 
CATGAGAAAC CCTCAAGGCA GAGTTCTAGT AAATCGGTTG GCGATTCTGA TGAAGTAGGT 
GGAAAAAAGA AAAAAAAGAA GCATACTAAA AGGAGGAAAA CTAGAAGTCC AAGCATAGAT 
GACAGATCTG AGGGCCACAG TCAGAGACAT CACAAGAGGA AGAAGAAACA CAAGAAGAAG 
AGCAAGAGAC ATAACAGTAA GGAGAGGACG AGAAAGCTCA GCCCTGTTCT CATTACAATT 
ACTACTGACA GTGATGGTGA AAGTGCTGAC CCCAGTGAAC CCTCAACCAG CAGTGTTTGT 
GCCATTAGAT CTACTGTCTT AATAGCAAAC ATGGCTACAG AAGCTTTCCT AGACAATGAG 
CATCAGTCAA CAGCTGGAAC TGAAAATAGT TCTGTGGGTG ATGCAGGAAA TTACCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

toporsa ( XM_021479343.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335018.1 Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa05782
Clone ID Related Accession (Same CDS sequence)	NM_001305555.1 , XM_017357742.1 , XM_017357742.2 , XM_021479343.1 , XM_021479283.1
Accession Version	XM_021479343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase Topors isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCAC CACAGATAAA GGAGCGGCCA TCAGGTGTTG TGTTAAACAC AATCTCAAAA 
GGAGCATCTC CAGAATCAAA ATGCCCAATC TGCCTGGATC ACTTTAAAAA CATATCATAT 
CTTGATGTGT GCCTTCACAA GTTCTGCTTC TGCTGCATCC ATGAATGGTC CAAGAACAAA 
GCTGAATGCC CTTTATGCAA ACAGCCATTT AATTCATTCT ATCACACTAT TAAGTCTGAA 
GATGACTACA AGAGGTTTGA CCTGCGACCA ACTGAAAATG GCTCATTTGG AAACATGGCA 
GGGCAAAGAT TCAGGTATCG CACCACGCTT ACAGGTGACC GCAGACCAGT ACAGAGGCGA 
ACGTCTCCTC CTCCCGACCA TGGGATCATA TTTGAGGGTC TGAGAGGATC TCTTCCACAA 
CGGCACAACA GAGATCTACA TAGCATGATC AGGAGGCTGA CAGTAAGGCA GAGAAGAGAG 
AGTGAGGGTA GGTCTTTGCG AAGTCTTCAA GATCAAGAAG TGGTTAAGTT CAGACGAGCC 
TTGTACAGAA GAGGTGTGCA GGTCCAAAGC GTGCAGGATG GTGGGCGTAC GAGAGAGACC 
TCAGCAGAGT TCTTCAAAAG AAATCCTGCT TGCCTCCACA GGCTTGTACC TTGGCTGAGA 
CGAGAACTGA CTGTTCTGTA CGGTGCTCAT GGTTCTCTGG TCAACATTGT GCAGCACATC 
ATTATGACTC TGATCACTCG ACATAACTTG GACGACCAGG CTGTTCAACA TGAGCTTCGG 
CCCTTCTTAC TGTCCCACAC AGAGCACTTC TTACATGAGT TTCTCAGCTT TGCTCGATCG 
CCGTTCAATA TGGAGGCATA TGACCAACGT GCTGTTTATG ACTTGCCAAG ACCTTCAGGA 
GAAAGTAGCA GTTCGGAAAA CTCTGTGATC GCCATCTCTG AGGATGAGAG CGAATCTTTA 
GTGTCAGGAG CCCAGGATTT TGCCACTCCT AGGGTGACCT CAAGCCAAAC AGCATGGGAT 
GATGAGACTC CAGGACCATC CTACTCTTCA GAGCGTTCTC AGGTGTTACC CGTCCACGTG 
AGGGACTCAG ACTCTGATAG TAGTGTAGGG GAAGCAGTAA TGCCTATTGC TGCAGTACGA 
CATCAGGACC ATCCAGCAAA TCCTTCTACT ATTCAACCAG AAGAGGATCA TTCCTCTAGC 
AATGATGAGG ACTGTGTTAT TATTGGCTAT GTTAAGCCCG TGGCAGAGAG GACACCAGAA 
CTGGTCCAGC TTTCTTCTGA TTCTGAAAAT TCCGAGACTG AGCAGAATGC AAGTCCACGA 
AACTCTCAAG CTCCCCAACA CATTCGTTTT ATTTCAGATT CAGATGCATC ACCCACTGCC 
TCACAGAGAC CCTTGAGGGG GCCAGCAAGT TCTTCACGCT CATTTGAAGA CACCTCCATA 
CAACTTAGAA AAAACAGTAA ATATGACAAT ACTTTTTCAG GATCTAGAGA AGGATCCTCA 
CAAAGCAAAA GGATACACTC TTCGTCTCAT AGAAAGAAGC AACCATTGTG TAAAGAGAGC 
AAGAATAAGC AGAGAGGGGA AAAGGAAACA AGTCGTCACT CTTCATCCTA TTGGCATTCT 
TATAGCTATT ACAGTCATGA CAACAGCAGA AAAGCTTATA CAGAAACAAG CTCATCTTAT 
ACAAGCTACT ACAGAAGTGT AAATGATCAG TTTGGTTCTC GATCACACGG CAAAAGAAGA 
TGTAGGGACC TTCTGGACAG AAGTCGCTCA AGGAGTAATT CAAGAATAAG CCAGTCGAAT 
CGGCGTAATA GGAGTCGCTC AAGGAGTAAT TCAAGAATAA GCCGGTCCAA TCAACGGAAT 
AGAAGTCACT CAAGGAGTAA TTCTGGAAAA AGCCAATCCA ATCGGCGTAA TAGAAGTTGT 
TCAAGGAGTT ATTCAAGAAC AAGCCCATTT AATGAACGGA ATAGAAGACA CTCAAGGAGT 
AATTCTAGAA AAAGCCAATC CAATCGGCAT AATAGAAGTT GTTCAAGGAG TTATTCAAGA 
ACAAGCCCAT TTAATGAACG GAATAGAAGA CACTCAAGGA GTAATTCTAG AAAAAGCCAA 
TCCAATCGGC ATAATAGAAG TTGTTCAAGG AGTTATTCAA GAACAAGCCC ATTTAATCAA 
CGGAATAGAA GACACTCAAG GAGTAATTCT AGAATAAGCC AGTCCAGTCG GCAGAACAGA 
AGTCTCTCAA GGAGTAATTC TAGAAAAGGC TATTCAAATC GGCGGAACAG AAATCATTCT 
AGGAGTAGTT CTAGAGTAAG CCAGTCCAGT CAGCGGAACA GAAGTCACTC AAGGAGTAAT 
TTTAGAATCA AGTCTAGCTC CTACAAAAGT CAAGATGAAA AATATCAAAG AAGCCTTTCT 
AGATCCCTTT ACTCCAATAA TGATGTCAGA AGTCGCTCAA GAAGCAGTTC CAGAATAAAC 
ACCTCTAGCC GCCAGGACCG AAGACGCTCA AGGAGCAGTT CTAGAATGAA TTCCAGCTCC 
TCACGCCAGA CATCATGCCA CGATAAATAT GGTAAAAAAA GGATGTACAA AACAAAGCAT 
CATGAGAAAC CCTCAAGGCA GAGTTCTAGT AAATCGGTTG GCGATTCTGA TGAAGTAGGT 
GGAAAAAAGA AAAAAAAGAA GCATACTAAA AGGAGGAAAA CTAGAAGTCC AAGCATAGAT 
GACAGATCTG AGGGCCACAG TCAGAGACAT CACAAGAGGA AGAAGAAACA CAAGAAGAAG 
AGCAAGAGAC ATAACAGTAA GGAGAGGACG AGAAAGCTCA GCCCTGTTCT CATTACAATT 
ACTACTGACA GTGATGGTGA AAGTGCTGAC CCCAGTGAAC CCTCAACCAG CAGTGTTTGT 
GCCATTAGAT CTACTGTCTT AATAGCAAAC ATGGCTACAG AAGCTTTCCT AGACAATGAG 
CATCAGTCAA CAGCTGGAAC TGAAAATAGT TCTGTGGGTG ATGCAGGAAA TTACCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335018.1
CDS	233..3232
Translation	MASPQIKERPSGVVLNTISKGASPESKCPICLDHFKNISYLDVCLHKFCFCCIHEWSKNKAECPLCKQPFNSFYHTIKSEDDYKRFDLRPTENGSFGNMAGQRFRYRTTLTGDRRPVQRRTSPPPDHGIIFEGLRGSLPQRHNRDLHSMIRRLTVRQRRESEGRSLRSLQDQEVVKFRRALYRRGVQVQSVQDGGRTRETSAEFFKRNPACLHRLVPWLRRELTVLYGAHGSLVNIVQHIIMTLITRHNLDDQAVQHELRPFLLSHTEHFLHEFLSFARSPFNMEAYDQRAVYDLPRPSGESSSSENSVIAISEDESESLVSGAQDFATPRVTSSQTAWDDETPGPSYSSERSQVLPVHVRDSDSDSSVGEAVMPIAAVRHQDHPANPSTIQPEEDHSSSNDEDCVIIGYVKPVAERTPELVQLSSDSENSETEQNASPRNSQAPQHIRFISDSDASPTASQRPLRGPASSSRSFEDTSIQLRKNSKYDNTFSGSREGSSQSKRIHSSSHRKKQPLCKESKNKQRGEKETSRHSSSYWHSYSYYSHDNSRKAYTETSSSYTSYYRSVNDQFGSRSHGKRRCRDLLDRSRSRSNSRISQSNRRNRSRSRSNSRISRSNQRNRSHSRSNSGKSQSNRRNRSCSRSYSRTSPFNERNRRHSRSNSRKSQSNRHNRSCSRSYSRTSPFNERNRRHSRSNSRKSQSNRHNRSCSRSYSRTSPFNQRNRRHSRSNSRISQSSRQNRSLSRSNSRKGYSNRRNRNHSRSSSRVSQSSQRNRSHSRSNFRIKSSSYKSQDEKYQRSLSRSLYSNNDVRSRSRSSSRINTSSRQDRRRSRSSSRMNSSSSRQTSCHDKYGKKRMYKTKHHEKPSRQSSSKSVGDSDEVGGKKKKKKHTKRRKTRSPSIDDRSEGHSQRHHKRKKKHKKKSKRHNSKERTRKLSPVLITITTDSDGESADPSEPSTSSVCAIRSTVLIANMATEAFLDNEHQSTAGTENSSVGDAGNYP

Target ORF information:

RefSeq Version	XM_021479343.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479343.1

ORF Insert Sequence:

ATGGCATCAC CACAGATAAA GGAGCGGCCA TCAGGTGTTG TGTTAAACAC AATCTCAAAA 
GGAGCATCTC CAGAATCAAA ATGCCCAATC TGCCTGGATC ACTTTAAAAA CATATCATAT 
CTTGATGTGT GCCTTCACAA GTTCTGCTTC TGCTGCATCC ATGAATGGTC CAAGAACAAA 
GCTGAATGCC CTTTATGCAA ACAGCCATTT AATTCATTCT ATCACACTAT TAAGTCTGAA 
GATGACTACA AGAGGTTTGA CCTGCGACCA ACTGAAAATG GCTCATTTGG AAACATGGCA 
GGGCAAAGAT TCAGGTATCG CACCACGCTT ACAGGTGACC GCAGACCAGT ACAGAGGCGA 
ACGTCTCCTC CTCCCGACCA TGGGATCATA TTTGAGGGTC TGAGAGGATC TCTTCCACAA 
CGGCACAACA GAGATCTACA TAGCATGATC AGGAGGCTGA CAGTAAGGCA GAGAAGAGAG 
AGTGAGGGTA GGTCTTTGCG AAGTCTTCAA GATCAAGAAG TGGTTAAGTT CAGACGAGCC 
TTGTACAGAA GAGGTGTGCA GGTCCAAAGC GTGCAGGATG GTGGGCGTAC GAGAGAGACC 
TCAGCAGAGT TCTTCAAAAG AAATCCTGCT TGCCTCCACA GGCTTGTACC TTGGCTGAGA 
CGAGAACTGA CTGTTCTGTA CGGTGCTCAT GGTTCTCTGG TCAACATTGT GCAGCACATC 
ATTATGACTC TGATCACTCG ACATAACTTG GACGACCAGG CTGTTCAACA TGAGCTTCGG 
CCCTTCTTAC TGTCCCACAC AGAGCACTTC TTACATGAGT TTCTCAGCTT TGCTCGATCG 
CCGTTCAATA TGGAGGCATA TGACCAACGT GCTGTTTATG ACTTGCCAAG ACCTTCAGGA 
GAAAGTAGCA GTTCGGAAAA CTCTGTGATC GCCATCTCTG AGGATGAGAG CGAATCTTTA 
GTGTCAGGAG CCCAGGATTT TGCCACTCCT AGGGTGACCT CAAGCCAAAC AGCATGGGAT 
GATGAGACTC CAGGACCATC CTACTCTTCA GAGCGTTCTC AGGTGTTACC CGTCCACGTG 
AGGGACTCAG ACTCTGATAG TAGTGTAGGG GAAGCAGTAA TGCCTATTGC TGCAGTACGA 
CATCAGGACC ATCCAGCAAA TCCTTCTACT ATTCAACCAG AAGAGGATCA TTCCTCTAGC 
AATGATGAGG ACTGTGTTAT TATTGGCTAT GTTAAGCCCG TGGCAGAGAG GACACCAGAA 
CTGGTCCAGC TTTCTTCTGA TTCTGAAAAT TCCGAGACTG AGCAGAATGC AAGTCCACGA 
AACTCTCAAG CTCCCCAACA CATTCGTTTT ATTTCAGATT CAGATGCATC ACCCACTGCC 
TCACAGAGAC CCTTGAGGGG GCCAGCAAGT TCTTCACGCT CATTTGAAGA CACCTCCATA 
CAACTTAGAA AAAACAGTAA ATATGACAAT ACTTTTTCAG GATCTAGAGA AGGATCCTCA 
CAAAGCAAAA GGATACACTC TTCGTCTCAT AGAAAGAAGC AACCATTGTG TAAAGAGAGC 
AAGAATAAGC AGAGAGGGGA AAAGGAAACA AGTCGTCACT CTTCATCCTA TTGGCATTCT 
TATAGCTATT ACAGTCATGA CAACAGCAGA AAAGCTTATA CAGAAACAAG CTCATCTTAT 
ACAAGCTACT ACAGAAGTGT AAATGATCAG TTTGGTTCTC GATCACACGG CAAAAGAAGA 
TGTAGGGACC TTCTGGACAG AAGTCGCTCA AGGAGTAATT CAAGAATAAG CCAGTCGAAT 
CGGCGTAATA GGAGTCGCTC AAGGAGTAAT TCAAGAATAA GCCGGTCCAA TCAACGGAAT 
AGAAGTCACT CAAGGAGTAA TTCTGGAAAA AGCCAATCCA ATCGGCGTAA TAGAAGTTGT 
TCAAGGAGTT ATTCAAGAAC AAGCCCATTT AATGAACGGA ATAGAAGACA CTCAAGGAGT 
AATTCTAGAA AAAGCCAATC CAATCGGCAT AATAGAAGTT GTTCAAGGAG TTATTCAAGA 
ACAAGCCCAT TTAATGAACG GAATAGAAGA CACTCAAGGA GTAATTCTAG AAAAAGCCAA 
TCCAATCGGC ATAATAGAAG TTGTTCAAGG AGTTATTCAA GAACAAGCCC ATTTAATCAA 
CGGAATAGAA GACACTCAAG GAGTAATTCT AGAATAAGCC AGTCCAGTCG GCAGAACAGA 
AGTCTCTCAA GGAGTAATTC TAGAAAAGGC TATTCAAATC GGCGGAACAG AAATCATTCT 
AGGAGTAGTT CTAGAGTAAG CCAGTCCAGT CAGCGGAACA GAAGTCACTC AAGGAGTAAT 
TTTAGAATCA AGTCTAGCTC CTACAAAAGT CAAGATGAAA AATATCAAAG AAGCCTTTCT 
AGATCCCTTT ACTCCAATAA TGATGTCAGA AGTCGCTCAA GAAGCAGTTC CAGAATAAAC 
ACCTCTAGCC GCCAGGACCG AAGACGCTCA AGGAGCAGTT CTAGAATGAA TTCCAGCTCC 
TCACGCCAGA CATCATGCCA CGATAAATAT GGTAAAAAAA GGATGTACAA AACAAAGCAT 
CATGAGAAAC CCTCAAGGCA GAGTTCTAGT AAATCGGTTG GCGATTCTGA TGAAGTAGGT 
GGAAAAAAGA AAAAAAAGAA GCATACTAAA AGGAGGAAAA CTAGAAGTCC AAGCATAGAT 
GACAGATCTG AGGGCCACAG TCAGAGACAT CACAAGAGGA AGAAGAAACA CAAGAAGAAG 
AGCAAGAGAC ATAACAGTAA GGAGAGGACG AGAAAGCTCA GCCCTGTTCT CATTACAATT 
ACTACTGACA GTGATGGTGA AAGTGCTGAC CCCAGTGAAC CCTCAACCAG CAGTGTTTGT 
GCCATTAGAT CTACTGTCTT AATAGCAAAC ATGGCTACAG AAGCTTTCCT AGACAATGAG 
CATCAGTCAA CAGCTGGAAC TGAAAATAGT TCTGTGGGTG ATGCAGGAAA TTACCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

toporsa ( XM_021479283.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334958.1 Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), transcript variant X1, mRNA.

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CloneID	ODa05782
Clone ID Related Accession (Same CDS sequence)	NM_001305555.1 , XM_017357742.1 , XM_017357742.2 , XM_021479343.1 , XM_021479283.1
Accession Version	XM_021479283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase Topors isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCAC CACAGATAAA GGAGCGGCCA TCAGGTGTTG TGTTAAACAC AATCTCAAAA 
GGAGCATCTC CAGAATCAAA ATGCCCAATC TGCCTGGATC ACTTTAAAAA CATATCATAT 
CTTGATGTGT GCCTTCACAA GTTCTGCTTC TGCTGCATCC ATGAATGGTC CAAGAACAAA 
GCTGAATGCC CTTTATGCAA ACAGCCATTT AATTCATTCT ATCACACTAT TAAGTCTGAA 
GATGACTACA AGAGGTTTGA CCTGCGACCA ACTGAAAATG GCTCATTTGG AAACATGGCA 
GGGCAAAGAT TCAGGTATCG CACCACGCTT ACAGGTGACC GCAGACCAGT ACAGAGGCGA 
ACGTCTCCTC CTCCCGACCA TGGGATCATA TTTGAGGGTC TGAGAGGATC TCTTCCACAA 
CGGCACAACA GAGATCTACA TAGCATGATC AGGAGGCTGA CAGTAAGGCA GAGAAGAGAG 
AGTGAGGGTA GGTCTTTGCG AAGTCTTCAA GATCAAGAAG TGGTTAAGTT CAGACGAGCC 
TTGTACAGAA GAGGTGTGCA GGTCCAAAGC GTGCAGGATG GTGGGCGTAC GAGAGAGACC 
TCAGCAGAGT TCTTCAAAAG AAATCCTGCT TGCCTCCACA GGCTTGTACC TTGGCTGAGA 
CGAGAACTGA CTGTTCTGTA CGGTGCTCAT GGTTCTCTGG TCAACATTGT GCAGCACATC 
ATTATGACTC TGATCACTCG ACATAACTTG GACGACCAGG CTGTTCAACA TGAGCTTCGG 
CCCTTCTTAC TGTCCCACAC AGAGCACTTC TTACATGAGT TTCTCAGCTT TGCTCGATCG 
CCGTTCAATA TGGAGGCATA TGACCAACGT GCTGTTTATG ACTTGCCAAG ACCTTCAGGA 
GAAAGTAGCA GTTCGGAAAA CTCTGTGATC GCCATCTCTG AGGATGAGAG CGAATCTTTA 
GTGTCAGGAG CCCAGGATTT TGCCACTCCT AGGGTGACCT CAAGCCAAAC AGCATGGGAT 
GATGAGACTC CAGGACCATC CTACTCTTCA GAGCGTTCTC AGGTGTTACC CGTCCACGTG 
AGGGACTCAG ACTCTGATAG TAGTGTAGGG GAAGCAGTAA TGCCTATTGC TGCAGTACGA 
CATCAGGACC ATCCAGCAAA TCCTTCTACT ATTCAACCAG AAGAGGATCA TTCCTCTAGC 
AATGATGAGG ACTGTGTTAT TATTGGCTAT GTTAAGCCCG TGGCAGAGAG GACACCAGAA 
CTGGTCCAGC TTTCTTCTGA TTCTGAAAAT TCCGAGACTG AGCAGAATGC AAGTCCACGA 
AACTCTCAAG CTCCCCAACA CATTCGTTTT ATTTCAGATT CAGATGCATC ACCCACTGCC 
TCACAGAGAC CCTTGAGGGG GCCAGCAAGT TCTTCACGCT CATTTGAAGA CACCTCCATA 
CAACTTAGAA AAAACAGTAA ATATGACAAT ACTTTTTCAG GATCTAGAGA AGGATCCTCA 
CAAAGCAAAA GGATACACTC TTCGTCTCAT AGAAAGAAGC AACCATTGTG TAAAGAGAGC 
AAGAATAAGC AGAGAGGGGA AAAGGAAACA AGTCGTCACT CTTCATCCTA TTGGCATTCT 
TATAGCTATT ACAGTCATGA CAACAGCAGA AAAGCTTATA CAGAAACAAG CTCATCTTAT 
ACAAGCTACT ACAGAAGTGT AAATGATCAG TTTGGTTCTC GATCACACGG CAAAAGAAGA 
TGTAGGGACC TTCTGGACAG AAGTCGCTCA AGGAGTAATT CAAGAATAAG CCAGTCGAAT 
CGGCGTAATA GGAGTCGCTC AAGGAGTAAT TCAAGAATAA GCCGGTCCAA TCAACGGAAT 
AGAAGTCACT CAAGGAGTAA TTCTGGAAAA AGCCAATCCA ATCGGCGTAA TAGAAGTTGT 
TCAAGGAGTT ATTCAAGAAC AAGCCCATTT AATGAACGGA ATAGAAGACA CTCAAGGAGT 
AATTCTAGAA AAAGCCAATC CAATCGGCAT AATAGAAGTT GTTCAAGGAG TTATTCAAGA 
ACAAGCCCAT TTAATGAACG GAATAGAAGA CACTCAAGGA GTAATTCTAG AAAAAGCCAA 
TCCAATCGGC ATAATAGAAG TTGTTCAAGG AGTTATTCAA GAACAAGCCC ATTTAATCAA 
CGGAATAGAA GACACTCAAG GAGTAATTCT AGAATAAGCC AGTCCAGTCG GCAGAACAGA 
AGTCTCTCAA GGAGTAATTC TAGAAAAGGC TATTCAAATC GGCGGAACAG AAATCATTCT 
AGGAGTAGTT CTAGAGTAAG CCAGTCCAGT CAGCGGAACA GAAGTCACTC AAGGAGTAAT 
TTTAGAATCA AGTCTAGCTC CTACAAAAGT CAAGATGAAA AATATCAAAG AAGCCTTTCT 
AGATCCCTTT ACTCCAATAA TGATGTCAGA AGTCGCTCAA GAAGCAGTTC CAGAATAAAC 
ACCTCTAGCC GCCAGGACCG AAGACGCTCA AGGAGCAGTT CTAGAATGAA TTCCAGCTCC 
TCACGCCAGA CATCATGCCA CGATAAATAT GGTAAAAAAA GGATGTACAA AACAAAGCAT 
CATGAGAAAC CCTCAAGGCA GAGTTCTAGT AAATCGGTTG GCGATTCTGA TGAAGTAGGT 
GGAAAAAAGA AAAAAAAGAA GCATACTAAA AGGAGGAAAA CTAGAAGTCC AAGCATAGAT 
GACAGATCTG AGGGCCACAG TCAGAGACAT CACAAGAGGA AGAAGAAACA CAAGAAGAAG 
AGCAAGAGAC ATAACAGTAA GGAGAGGACG AGAAAGCTCA GCCCTGTTCT CATTACAATT 
ACTACTGACA GTGATGGTGA AAGTGCTGAC CCCAGTGAAC CCTCAACCAG CAGTGTTTGT 
GCCATTAGAT CTACTGTCTT AATAGCAAAC ATGGCTACAG AAGCTTTCCT AGACAATGAG 
CATCAGTCAA CAGCTGGAAC TGAAAATAGT TCTGTGGGTG ATGCAGGAAA TTACCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334958.1
CDS	2124..5123
Translation	MASPQIKERPSGVVLNTISKGASPESKCPICLDHFKNISYLDVCLHKFCFCCIHEWSKNKAECPLCKQPFNSFYHTIKSEDDYKRFDLRPTENGSFGNMAGQRFRYRTTLTGDRRPVQRRTSPPPDHGIIFEGLRGSLPQRHNRDLHSMIRRLTVRQRRESEGRSLRSLQDQEVVKFRRALYRRGVQVQSVQDGGRTRETSAEFFKRNPACLHRLVPWLRRELTVLYGAHGSLVNIVQHIIMTLITRHNLDDQAVQHELRPFLLSHTEHFLHEFLSFARSPFNMEAYDQRAVYDLPRPSGESSSSENSVIAISEDESESLVSGAQDFATPRVTSSQTAWDDETPGPSYSSERSQVLPVHVRDSDSDSSVGEAVMPIAAVRHQDHPANPSTIQPEEDHSSSNDEDCVIIGYVKPVAERTPELVQLSSDSENSETEQNASPRNSQAPQHIRFISDSDASPTASQRPLRGPASSSRSFEDTSIQLRKNSKYDNTFSGSREGSSQSKRIHSSSHRKKQPLCKESKNKQRGEKETSRHSSSYWHSYSYYSHDNSRKAYTETSSSYTSYYRSVNDQFGSRSHGKRRCRDLLDRSRSRSNSRISQSNRRNRSRSRSNSRISRSNQRNRSHSRSNSGKSQSNRRNRSCSRSYSRTSPFNERNRRHSRSNSRKSQSNRHNRSCSRSYSRTSPFNERNRRHSRSNSRKSQSNRHNRSCSRSYSRTSPFNQRNRRHSRSNSRISQSSRQNRSLSRSNSRKGYSNRRNRNHSRSSSRVSQSSQRNRSHSRSNFRIKSSSYKSQDEKYQRSLSRSLYSNNDVRSRSRSSSRINTSSRQDRRRSRSSSRMNSSSSRQTSCHDKYGKKRMYKTKHHEKPSRQSSSKSVGDSDEVGGKKKKKKHTKRRKTRSPSIDDRSEGHSQRHHKRKKKHKKKSKRHNSKERTRKLSPVLITITTDSDGESADPSEPSTSSVCAIRSTVLIANMATEAFLDNEHQSTAGTENSSVGDAGNYP

Target ORF information:

RefSeq Version	XM_021479283.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio topoisomerase I binding, arginine/serine-rich a (toporsa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479283.1

ORF Insert Sequence:

ATGGCATCAC CACAGATAAA GGAGCGGCCA TCAGGTGTTG TGTTAAACAC AATCTCAAAA 
GGAGCATCTC CAGAATCAAA ATGCCCAATC TGCCTGGATC ACTTTAAAAA CATATCATAT 
CTTGATGTGT GCCTTCACAA GTTCTGCTTC TGCTGCATCC ATGAATGGTC CAAGAACAAA 
GCTGAATGCC CTTTATGCAA ACAGCCATTT AATTCATTCT ATCACACTAT TAAGTCTGAA 
GATGACTACA AGAGGTTTGA CCTGCGACCA ACTGAAAATG GCTCATTTGG AAACATGGCA 
GGGCAAAGAT TCAGGTATCG CACCACGCTT ACAGGTGACC GCAGACCAGT ACAGAGGCGA 
ACGTCTCCTC CTCCCGACCA TGGGATCATA TTTGAGGGTC TGAGAGGATC TCTTCCACAA 
CGGCACAACA GAGATCTACA TAGCATGATC AGGAGGCTGA CAGTAAGGCA GAGAAGAGAG 
AGTGAGGGTA GGTCTTTGCG AAGTCTTCAA GATCAAGAAG TGGTTAAGTT CAGACGAGCC 
TTGTACAGAA GAGGTGTGCA GGTCCAAAGC GTGCAGGATG GTGGGCGTAC GAGAGAGACC 
TCAGCAGAGT TCTTCAAAAG AAATCCTGCT TGCCTCCACA GGCTTGTACC TTGGCTGAGA 
CGAGAACTGA CTGTTCTGTA CGGTGCTCAT GGTTCTCTGG TCAACATTGT GCAGCACATC 
ATTATGACTC TGATCACTCG ACATAACTTG GACGACCAGG CTGTTCAACA TGAGCTTCGG 
CCCTTCTTAC TGTCCCACAC AGAGCACTTC TTACATGAGT TTCTCAGCTT TGCTCGATCG 
CCGTTCAATA TGGAGGCATA TGACCAACGT GCTGTTTATG ACTTGCCAAG ACCTTCAGGA 
GAAAGTAGCA GTTCGGAAAA CTCTGTGATC GCCATCTCTG AGGATGAGAG CGAATCTTTA 
GTGTCAGGAG CCCAGGATTT TGCCACTCCT AGGGTGACCT CAAGCCAAAC AGCATGGGAT 
GATGAGACTC CAGGACCATC CTACTCTTCA GAGCGTTCTC AGGTGTTACC CGTCCACGTG 
AGGGACTCAG ACTCTGATAG TAGTGTAGGG GAAGCAGTAA TGCCTATTGC TGCAGTACGA 
CATCAGGACC ATCCAGCAAA TCCTTCTACT ATTCAACCAG AAGAGGATCA TTCCTCTAGC 
AATGATGAGG ACTGTGTTAT TATTGGCTAT GTTAAGCCCG TGGCAGAGAG GACACCAGAA 
CTGGTCCAGC TTTCTTCTGA TTCTGAAAAT TCCGAGACTG AGCAGAATGC AAGTCCACGA 
AACTCTCAAG CTCCCCAACA CATTCGTTTT ATTTCAGATT CAGATGCATC ACCCACTGCC 
TCACAGAGAC CCTTGAGGGG GCCAGCAAGT TCTTCACGCT CATTTGAAGA CACCTCCATA 
CAACTTAGAA AAAACAGTAA ATATGACAAT ACTTTTTCAG GATCTAGAGA AGGATCCTCA 
CAAAGCAAAA GGATACACTC TTCGTCTCAT AGAAAGAAGC AACCATTGTG TAAAGAGAGC 
AAGAATAAGC AGAGAGGGGA AAAGGAAACA AGTCGTCACT CTTCATCCTA TTGGCATTCT 
TATAGCTATT ACAGTCATGA CAACAGCAGA AAAGCTTATA CAGAAACAAG CTCATCTTAT 
ACAAGCTACT ACAGAAGTGT AAATGATCAG TTTGGTTCTC GATCACACGG CAAAAGAAGA 
TGTAGGGACC TTCTGGACAG AAGTCGCTCA AGGAGTAATT CAAGAATAAG CCAGTCGAAT 
CGGCGTAATA GGAGTCGCTC AAGGAGTAAT TCAAGAATAA GCCGGTCCAA TCAACGGAAT 
AGAAGTCACT CAAGGAGTAA TTCTGGAAAA AGCCAATCCA ATCGGCGTAA TAGAAGTTGT 
TCAAGGAGTT ATTCAAGAAC AAGCCCATTT AATGAACGGA ATAGAAGACA CTCAAGGAGT 
AATTCTAGAA AAAGCCAATC CAATCGGCAT AATAGAAGTT GTTCAAGGAG TTATTCAAGA 
ACAAGCCCAT TTAATGAACG GAATAGAAGA CACTCAAGGA GTAATTCTAG AAAAAGCCAA 
TCCAATCGGC ATAATAGAAG TTGTTCAAGG AGTTATTCAA GAACAAGCCC ATTTAATCAA 
CGGAATAGAA GACACTCAAG GAGTAATTCT AGAATAAGCC AGTCCAGTCG GCAGAACAGA 
AGTCTCTCAA GGAGTAATTC TAGAAAAGGC TATTCAAATC GGCGGAACAG AAATCATTCT 
AGGAGTAGTT CTAGAGTAAG CCAGTCCAGT CAGCGGAACA GAAGTCACTC AAGGAGTAAT 
TTTAGAATCA AGTCTAGCTC CTACAAAAGT CAAGATGAAA AATATCAAAG AAGCCTTTCT 
AGATCCCTTT ACTCCAATAA TGATGTCAGA AGTCGCTCAA GAAGCAGTTC CAGAATAAAC 
ACCTCTAGCC GCCAGGACCG AAGACGCTCA AGGAGCAGTT CTAGAATGAA TTCCAGCTCC 
TCACGCCAGA CATCATGCCA CGATAAATAT GGTAAAAAAA GGATGTACAA AACAAAGCAT 
CATGAGAAAC CCTCAAGGCA GAGTTCTAGT AAATCGGTTG GCGATTCTGA TGAAGTAGGT 
GGAAAAAAGA AAAAAAAGAA GCATACTAAA AGGAGGAAAA CTAGAAGTCC AAGCATAGAT 
GACAGATCTG AGGGCCACAG TCAGAGACAT CACAAGAGGA AGAAGAAACA CAAGAAGAAG 
AGCAAGAGAC ATAACAGTAA GGAGAGGACG AGAAAGCTCA GCCCTGTTCT CATTACAATT 
ACTACTGACA GTGATGGTGA AAGTGCTGAC CCCAGTGAAC CCTCAACCAG CAGTGTTTGT 
GCCATTAGAT CTACTGTCTT AATAGCAAAC ATGGCTACAG AAGCTTTCCT AGACAATGAG 
CATCAGTCAA CAGCTGGAAC TGAAAATAGT TCTGTGGGTG ATGCAGGAAA TTACCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

TOPORS, implicated in retinal degeneration, is a cilia-centrosomal protein.
Human molecular genetics20(5)975-87(2011 Mar)
Chakarova CF,Khanna H,Shah AZ,Patil SB,Sedmak T,Murga-Zamalloa CA,Papaioannou MG,Nagel-Wolfrum K,Lopez I,Munro P,Cheetham M,Koenekoop RK,Rios RM,Matter K,Wolfrum U,Swaroop A,Bhattacharya SS

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toporsa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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