wu:fb77a09 cDNA ORF clone, Danio rerio(zebrafish)

The following wu:fb77a09 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wu:fb77a09 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa149665 XM_021467329.1
Latest version!
Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa22350 XM_005169847.3
Latest version!
Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa22351 XM_005169848.3
Latest version!
Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa22352 XM_005169849.3
Latest version!
Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa22353 XM_005169850.3
Latest version!
Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa22355 XM_005169852.3
Latest version!
Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa55694 XM_017351774.2
Latest version!
Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
ODa22350 XM_005169847.2 Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa22353 XM_005169850.2 Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa22351 XM_005169848.2 Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa55694 XM_017351774.1 Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa22352 XM_005169849.2 Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa22355 XM_005169852.2 Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa149665
    Clone ID Related Accession (Same CDS sequence) XM_021467329.1
    Accession Version XM_021467329.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nidogen-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTTCCAAG
    ACCCACTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCCAGAGTAA ACTGTGATCA ACCTGTTCGT CCCAAGACCC AGTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGGAGACGG TGGTGTTCCT GCGGTCGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAGTAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTGATAGT TCCTCCAACG CAGCGTCCGG AGAGTGTCTG TGAGCGCTGG
    AGAGCAAGTC TGTTACAGCA GTATGGAGGT CAGCCAGATT CCCAGCATTA TCTACCTCAG
    TGTGACTCTG CCGGTGAGTT CAACCCAGTT CAGTGTTATG GAGGCAGCAG CTACTGCTGG
    TGTGTGGACG CAAATGGACG AGAAGTACCA GGAACCCGCT CACATGATGC TGTGAAGCCT
    GCCTGTATAC CTACTGTGGC CTCGCCTACG GTGCACCCTC TCCCACGCCC AGATGTGACT
    CCGCCTCCTA CGGGCACGTC TCTTCTCTAC GCCCAGGGCC AGCAGATTGG AGTTTTGCCT
    CTCAATGGCA CACAGATGGA TAAGGAGAGA TCGTCAGTGC TGCTCGCTTT ACATGGCTCG
    ATAGTGGTTG GCATTGATTA CGACTGCAGA GAAAGGAAAG TATACTGGAC AGATCTGGCA
    GGACGGACAA TCAATCGAGC CAGCCTGGAT CCAGGATCAG AACCAGAGAT CATCATCAAT
    ACAGCTCTGA CGAGTCCTGA AGGTCTGGCT GTGGATGTGG CCCGAAGGAG ATTGTTTTGG
    GTGGATAGCA CACTGGACAA GATCGAAACG GCCAATCTCG ATGGAAGTGA CAGACATGTT
    CTGTTCGCCA CAGATTTAGT AAATCCCAGA GCGATGATCG TGGACTCGTC TACAGGAACC
    CTCTACTGGA CTGACTGGAA CCGAGAGGCT CCTAAAATCG AGAGCTCTTC TGTGGATGGG
    CAAAACCGAA GGGTTCTGGT ACAGGAAGGC ATAGGACTGC CGAATGCCTT GACCTACGAC
    TCCACCATGC GTCAGGTCTG CTGGGCCGAT GCTGGAACCA AGCGTCTGGA GTGTATTTCA
    CCAAACGGGA CAGGCAGACG CGTGATCCAG AGTAACCTGA ACTACCCTTT CAGCATGGTG
    TACTTCGCCA ATCACTACTA CTACACTGAC TGGAGGAGGG ACGGAGTCAT CTCTCTAGGT
    CGAGATAACC AGTTAACAGA TGAGTACTTA CCTGATCAGA GGTCTCATTT GTATGGTATC
    ACAGTCGCCC CTCCACAATG TTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323004.1
    CDS32..5218
    Translation

    Target ORF information:

    RefSeq Version XM_021467329.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467329.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTTCCAAG
    ACCCACTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCCAGAGTAA ACTGTGATCA ACCTGTTCGT CCCAAGACCC AGTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGGAGACGG TGGTGTTCCT GCGGTCGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAGTAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTGATAGT TCCTCCAACG CAGCGTCCGG AGAGTGTCTG TGAGCGCTGG
    AGAGCAAGTC TGTTACAGCA GTATGGAGGT CAGCCAGATT CCCAGCATTA TCTACCTCAG
    TGTGACTCTG CCGGTGAGTT CAACCCAGTT CAGTGTTATG GAGGCAGCAG CTACTGCTGG
    TGTGTGGACG CAAATGGACG AGAAGTACCA GGAACCCGCT CACATGATGC TGTGAAGCCT
    GCCTGTATAC CTACTGTGGC CTCGCCTACG GTGCACCCTC TCCCACGCCC AGATGTGACT
    CCGCCTCCTA CGGGCACGTC TCTTCTCTAC GCCCAGGGCC AGCAGATTGG AGTTTTGCCT
    CTCAATGGCA CACAGATGGA TAAGGAGAGA TCGTCAGTGC TGCTCGCTTT ACATGGCTCG
    ATAGTGGTTG GCATTGATTA CGACTGCAGA GAAAGGAAAG TATACTGGAC AGATCTGGCA
    GGACGGACAA TCAATCGAGC CAGCCTGGAT CCAGGATCAG AACCAGAGAT CATCATCAAT
    ACAGCTCTGA CGAGTCCTGA AGGTCTGGCT GTGGATGTGG CCCGAAGGAG ATTGTTTTGG
    GTGGATAGCA CACTGGACAA GATCGAAACG GCCAATCTCG ATGGAAGTGA CAGACATGTT
    CTGTTCGCCA CAGATTTAGT AAATCCCAGA GCGATGATCG TGGACTCGTC TACAGGAACC
    CTCTACTGGA CTGACTGGAA CCGAGAGGCT CCTAAAATCG AGAGCTCTTC TGTGGATGGG
    CAAAACCGAA GGGTTCTGGT ACAGGAAGGC ATAGGACTGC CGAATGCCTT GACCTACGAC
    TCCACCATGC GTCAGGTCTG CTGGGCCGAT GCTGGAACCA AGCGTCTGGA GTGTATTTCA
    CCAAACGGGA CAGGCAGACG CGTGATCCAG AGTAACCTGA ACTACCCTTT CAGCATGGTG
    TACTTCGCCA ATCACTACTA CTACACTGAC TGGAGGAGGG ACGGAGTCAT CTCTCTAGGT
    CGAGATAACC AGTTAACAGA TGAGTACTTA CCTGATCAGA GGTCTCATTT GTATGGTATC
    ACAGTCGCCC CTCCACAATG TTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22350
    Clone ID Related Accession (Same CDS sequence) XM_005169847.3 , XM_005169847.2
    Accession Version XM_005169847.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nidogen-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169847.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTTCCAAG
    ACCCACTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCCAGAGTAA ACTGTGATCA ACCTGTTCGT CCCAAGACCC AGTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGGAAACGA TGGCGTTCCT GTGGTCGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGAC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGCTCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAG TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GTGTTCCTGC GGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAGTAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTGATAGTTC CTCCAACGCA GCGTCCGGAG AGTGTCTGTG AGCGCTGGAG AGCAAGTCTG
    TTACAGCAGT ATGGAGGTCA GCCAGATTCC CAGCATTATC TACCTCAGTG TGACTCTGCC
    GGTGAGTTCA ACCCAGTTCA GTGTTATGGA GGCAGCAGCT ACTGCTGGTG TGTGGACGCA
    AATGGACGAG AAGTACCAGG AACCCGCTCA CATGATGCTG TGAAGCCTGC CTGTATACCT
    ACTGTGGCCT CGCCTACGGT GCACCCTCTC CCACGCCCAG ATGTGACTCC GCCTCCTACG
    GGCACGTCTC TTCTCTACGC CCAGGGCCAG CAGATTGGAG TTTTGCCTCT CAATGGCACA
    CAGATGGATA AGGAGAGATC GTCAGTGCTG CTCGCTTTAC ATGGCTCGAT AGTGGTTGGC
    ATTGATTACG ACTGCAGAGA AAGGAAAGTA TACTGGACAG ATCTGGCAGG ACGGACAATC
    AATCGAGCCA GCCTGGATCC AGGATCAGAA CCAGAGATCA TCATCAATAC AGCTCTGACG
    AGTCCTGAAG GTCTGGCTGT GGATGTGGCC CGAAGGAGAT TGTTTTGGGT GGATAGCACA
    CTGGACAAGA TCGAAACGGC CAATCTCGAT GGAAGTGACA GACATGTTCT GTTCGCCACA
    GATTTAGTAA ATCCCAGAGC GATGATCGTG GACTCGTCTA CAGGAACCCT CTACTGGACT
    GACTGGAACC GAGAGGCTCC TAAAATCGAG AGCTCTTCTG TGGATGGGCA AAACCGAAGG
    GTTCTGGTAC AGGAAGGCAT AGGACTGCCG AATGCCTTGA CCTACGACTC CACCATGCGT
    CAGGTCTGCT GGGCCGATGC TGGAACCAAG CGTCTGGAGT GTATTTCACC AAACGGGACA
    GGCAGACGCG TGATCCAGAG TAACCTGAAC TACCCTTTCA GCATGGTGTA CTTCGCCAAT
    CACTACTACT ACACTGACTG GAGGAGGGAC GGAGTCATCT CTCTAGGTCG AGATAACCAG
    TTAACAGATG AGTACTTACC TGATCAGAGG TCTCATTTGT ATGGTATCAC AGTCGCCCCT
    CCACAATGTT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169904.1
    CDS31..5445
    Translation

    Target ORF information:

    RefSeq Version XM_005169847.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169847.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTTCCAAG
    ACCCACTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCCAGAGTAA ACTGTGATCA ACCTGTTCGT CCCAAGACCC AGTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGGAAACGA TGGCGTTCCT GTGGTCGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGAC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGCTCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAG TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GTGTTCCTGC GGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAGTAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTGATAGTTC CTCCAACGCA GCGTCCGGAG AGTGTCTGTG AGCGCTGGAG AGCAAGTCTG
    TTACAGCAGT ATGGAGGTCA GCCAGATTCC CAGCATTATC TACCTCAGTG TGACTCTGCC
    GGTGAGTTCA ACCCAGTTCA GTGTTATGGA GGCAGCAGCT ACTGCTGGTG TGTGGACGCA
    AATGGACGAG AAGTACCAGG AACCCGCTCA CATGATGCTG TGAAGCCTGC CTGTATACCT
    ACTGTGGCCT CGCCTACGGT GCACCCTCTC CCACGCCCAG ATGTGACTCC GCCTCCTACG
    GGCACGTCTC TTCTCTACGC CCAGGGCCAG CAGATTGGAG TTTTGCCTCT CAATGGCACA
    CAGATGGATA AGGAGAGATC GTCAGTGCTG CTCGCTTTAC ATGGCTCGAT AGTGGTTGGC
    ATTGATTACG ACTGCAGAGA AAGGAAAGTA TACTGGACAG ATCTGGCAGG ACGGACAATC
    AATCGAGCCA GCCTGGATCC AGGATCAGAA CCAGAGATCA TCATCAATAC AGCTCTGACG
    AGTCCTGAAG GTCTGGCTGT GGATGTGGCC CGAAGGAGAT TGTTTTGGGT GGATAGCACA
    CTGGACAAGA TCGAAACGGC CAATCTCGAT GGAAGTGACA GACATGTTCT GTTCGCCACA
    GATTTAGTAA ATCCCAGAGC GATGATCGTG GACTCGTCTA CAGGAACCCT CTACTGGACT
    GACTGGAACC GAGAGGCTCC TAAAATCGAG AGCTCTTCTG TGGATGGGCA AAACCGAAGG
    GTTCTGGTAC AGGAAGGCAT AGGACTGCCG AATGCCTTGA CCTACGACTC CACCATGCGT
    CAGGTCTGCT GGGCCGATGC TGGAACCAAG CGTCTGGAGT GTATTTCACC AAACGGGACA
    GGCAGACGCG TGATCCAGAG TAACCTGAAC TACCCTTTCA GCATGGTGTA CTTCGCCAAT
    CACTACTACT ACACTGACTG GAGGAGGGAC GGAGTCATCT CTCTAGGTCG AGATAACCAG
    TTAACAGATG AGTACTTACC TGATCAGAGG TCTCATTTGT ATGGTATCAC AGTCGCCCCT
    CCACAATGTT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22350
    Clone ID Related Accession (Same CDS sequence) XM_005169847.3 , XM_005169847.2
    Accession Version XM_005169847.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nidogen-2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169847.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTTCCAAG
    ACCCACTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCCAGAGTAA ACTGTGATCA ACCTGTTCGT CCCAAGACCC AGTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGGAAACGA TGGCGTTCCT GTGGTCGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGAC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGCTCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAG TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GTGTTCCTGC GGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAGTAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTGATAGTTC CTCCAACGCA GCGTCCGGAG AGTGTCTGTG AGCGCTGGAG AGCAAGTCTG
    TTACAGCAGT ATGGAGGTCA GCCAGATTCC CAGCATTATC TACCTCAGTG TGACTCTGCC
    GGTGAGTTCA ACCCAGTTCA GTGTTATGGA GGCAGCAGCT ACTGCTGGTG TGTGGACGCA
    AATGGACGAG AAGTACCAGG AACCCGCTCA CATGATGCTG TGAAGCCTGC CTGTATACCT
    ACTGTGGCCT CGCCTACGGT GCACCCTCTC CCACGCCCAG ATGTGACTCC GCCTCCTACG
    GGCACGTCTC TTCTCTACGC CCAGGGCCAG CAGATTGGAG TTTTGCCTCT CAATGGCACA
    CAGATGGATA AGGAGAGATC GTCAGTGCTG CTCGCTTTAC ATGGCTCGAT AGTGGTTGGC
    ATTGATTACG ACTGCAGAGA AAGGAAAGTA TACTGGACAG ATCTGGCAGG ACGGACAATC
    AATCGAGCCA GCCTGGATCC AGGATCAGAA CCAGAGATCA TCATCAATAC AGCTCTGACG
    AGTCCTGAAG GTCTGGCTGT GGATGTGGCC CGAAGGAGAT TGTTTTGGGT GGATAGCACA
    CTGGACAAGA TCGAAACGGC CAATCTCGAT GGAAGTGACA GACATGTTCT GTTCGCCACA
    GATTTAGTAA ATCCCAGAGC GATGATCGTG GACTCGTCTA CAGGAACCCT CTACTGGACT
    GACTGGAACC GAGAGGCTCC TAAAATCGAG AGCTCTTCTG TGGATGGGCA AAACCGAAGG
    GTTCTGGTAC AGGAAGGCAT AGGACTGCCG AATGCCTTGA CCTACGACTC CACCATGCGT
    CAGGTCTGCT GGGCCGATGC TGGAACCAAG CGTCTGGAGT GTATTTCACC AAACGGGACA
    GGCAGACGCG TGATCCAGAG TAACCTGAAC TACCCTTTCA GCATGGTGTA CTTCGCCAAT
    CACTACTACT ACACTGACTG GAGGAGGGAC GGAGTCATCT CTCTAGGTCG AGATAACCAG
    TTAACAGATG AGTACTTACC TGATCAGAGG TCTCATTTGT ATGGTATCAC AGTCGCCCCT
    CCACAATGTT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169904.1
    CDS5..5419
    Translation

    Target ORF information:

    RefSeq Version XM_005169847.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169847.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTTCCAAG
    ACCCACTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCCAGAGTAA ACTGTGATCA ACCTGTTCGT CCCAAGACCC AGTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGGAAACGA TGGCGTTCCT GTGGTCGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGAC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGCTCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAG TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GTGTTCCTGC GGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAGTAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTGATAGTTC CTCCAACGCA GCGTCCGGAG AGTGTCTGTG AGCGCTGGAG AGCAAGTCTG
    TTACAGCAGT ATGGAGGTCA GCCAGATTCC CAGCATTATC TACCTCAGTG TGACTCTGCC
    GGTGAGTTCA ACCCAGTTCA GTGTTATGGA GGCAGCAGCT ACTGCTGGTG TGTGGACGCA
    AATGGACGAG AAGTACCAGG AACCCGCTCA CATGATGCTG TGAAGCCTGC CTGTATACCT
    ACTGTGGCCT CGCCTACGGT GCACCCTCTC CCACGCCCAG ATGTGACTCC GCCTCCTACG
    GGCACGTCTC TTCTCTACGC CCAGGGCCAG CAGATTGGAG TTTTGCCTCT CAATGGCACA
    CAGATGGATA AGGAGAGATC GTCAGTGCTG CTCGCTTTAC ATGGCTCGAT AGTGGTTGGC
    ATTGATTACG ACTGCAGAGA AAGGAAAGTA TACTGGACAG ATCTGGCAGG ACGGACAATC
    AATCGAGCCA GCCTGGATCC AGGATCAGAA CCAGAGATCA TCATCAATAC AGCTCTGACG
    AGTCCTGAAG GTCTGGCTGT GGATGTGGCC CGAAGGAGAT TGTTTTGGGT GGATAGCACA
    CTGGACAAGA TCGAAACGGC CAATCTCGAT GGAAGTGACA GACATGTTCT GTTCGCCACA
    GATTTAGTAA ATCCCAGAGC GATGATCGTG GACTCGTCTA CAGGAACCCT CTACTGGACT
    GACTGGAACC GAGAGGCTCC TAAAATCGAG AGCTCTTCTG TGGATGGGCA AAACCGAAGG
    GTTCTGGTAC AGGAAGGCAT AGGACTGCCG AATGCCTTGA CCTACGACTC CACCATGCGT
    CAGGTCTGCT GGGCCGATGC TGGAACCAAG CGTCTGGAGT GTATTTCACC AAACGGGACA
    GGCAGACGCG TGATCCAGAG TAACCTGAAC TACCCTTTCA GCATGGTGTA CTTCGCCAAT
    CACTACTACT ACACTGACTG GAGGAGGGAC GGAGTCATCT CTCTAGGTCG AGATAACCAG
    TTAACAGATG AGTACTTACC TGATCAGAGG TCTCATTTGT ATGGTATCAC AGTCGCCCCT
    CCACAATGTT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22351
    Clone ID Related Accession (Same CDS sequence) XM_005169848.2 , XM_005169848.3
    Accession Version XM_005169848.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nidogen-2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCATTCCTGT GGTTGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAGTAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTTCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    GTTCCTGTGG TCGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA ATAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTCCCAAG
    ACCCAGTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAA ACGATGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGACGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGCTCA
    CCCAGAGTAA ACTGTGATCA ACCTGTTCGT CCCAAGACCC AGTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGGAGACGG TGGTGTTCCT GCGGTCGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAGTAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTGATAGT TCCTCCAACG CAGCGTCCGG AGAGTGTCTG TGAGCGCTGG
    AGAGCAAGTC TGTTACAGCA GTATGGAGGT CAGCCAGATT CCCAGCATTA TCTACCTCAG
    TGTGACTCTG CCGGTGAGTT CAACCCAGTT CAGTGTTATG GAGGCAGCAG CTACTGCTGG
    TGTGTGGACG CAAATGGACG AGAAGTACCA GGAACCCGCT CACATGATGC TGTGAAGCCT
    GCCTGTATAC CTACTGTGGC CTCGCCTACG GTGCACCCTC TCCCACGCCC AGATGTGACT
    CCGCCTCCTA CGGGCACGTC TCTTCTCTAC GCCCAGGGCC AGCAGATTGG AGTTTTGCCT
    CTCAATGGCA CACAGATGGA TAAGGAGAGA TCGTCAGTGC TGCTCGCTTT ACATGGCTCG
    ATAGTGGTTG GCATTGATTA CGACTGCAGA GAAAGGAAAG TATACTGGAC AGATCTGGCA
    GGACGGACAA TCAATCGAGC CAGCCTGGAT CCAGGATCAG AACCAGAGAT CATCATCAAT
    ACAGCTCTGA CGAGTCCTGA AGGTCTGGCT GTGGATGTGG CCCGAAGGAG ATTGTTTTGG
    GTGGATAGCA CACTGGACAA GATCGAAACG GCCAATCTCG ATGGAAGTGA CAGACATGTT
    CTGTTCGCCA CAGATTTAGT AAATCCCAGA GCGATGATCG TGGACTCGTC TACAGGAACC
    CTCTACTGGA CTGACTGGAA CCGAGAGGCT CCTAAAATCG AGAGCTCTTC TGTGGATGGG
    CAAAACCGAA GGGTTCTGGT ACAGGAAGGC ATAGGACTGC CGAATGCCTT GACCTACGAC
    TCCACCATGC GTCAGGTCTG CTGGGCCGAT GCTGGAACCA AGCGTCTGGA GTGTATTTCA
    CCAAACGGGA CAGGCAGACG CGTGATCCAG AGTAACCTGA ACTACCCTTT CAGCATGGTG
    TACTTCGCCA ATCACTACTA CTACACTGAC TGGAGGAGGG ACGGAGTCAT CTCTCTAGGT
    CGAGATAACC AGTTAACAGA TGAGTACTTA CCTGATCAGA GGTCTCATTT GTATGGTATC
    ACAGTCGCCC CTCCACAATG TTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169905.1
    CDS5..5191
    Translation

    Target ORF information:

    RefSeq Version XM_005169848.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169848.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCATTCCTGT GGTTGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAGTAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTTCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    GTTCCTGTGG TCGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA ATAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTCCCAAG
    ACCCAGTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAA ACGATGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGACGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGCTCA
    CCCAGAGTAA ACTGTGATCA ACCTGTTCGT CCCAAGACCC AGTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGGAGACGG TGGTGTTCCT GCGGTCGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAGTAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTGATAGT TCCTCCAACG CAGCGTCCGG AGAGTGTCTG TGAGCGCTGG
    AGAGCAAGTC TGTTACAGCA GTATGGAGGT CAGCCAGATT CCCAGCATTA TCTACCTCAG
    TGTGACTCTG CCGGTGAGTT CAACCCAGTT CAGTGTTATG GAGGCAGCAG CTACTGCTGG
    TGTGTGGACG CAAATGGACG AGAAGTACCA GGAACCCGCT CACATGATGC TGTGAAGCCT
    GCCTGTATAC CTACTGTGGC CTCGCCTACG GTGCACCCTC TCCCACGCCC AGATGTGACT
    CCGCCTCCTA CGGGCACGTC TCTTCTCTAC GCCCAGGGCC AGCAGATTGG AGTTTTGCCT
    CTCAATGGCA CACAGATGGA TAAGGAGAGA TCGTCAGTGC TGCTCGCTTT ACATGGCTCG
    ATAGTGGTTG GCATTGATTA CGACTGCAGA GAAAGGAAAG TATACTGGAC AGATCTGGCA
    GGACGGACAA TCAATCGAGC CAGCCTGGAT CCAGGATCAG AACCAGAGAT CATCATCAAT
    ACAGCTCTGA CGAGTCCTGA AGGTCTGGCT GTGGATGTGG CCCGAAGGAG ATTGTTTTGG
    GTGGATAGCA CACTGGACAA GATCGAAACG GCCAATCTCG ATGGAAGTGA CAGACATGTT
    CTGTTCGCCA CAGATTTAGT AAATCCCAGA GCGATGATCG TGGACTCGTC TACAGGAACC
    CTCTACTGGA CTGACTGGAA CCGAGAGGCT CCTAAAATCG AGAGCTCTTC TGTGGATGGG
    CAAAACCGAA GGGTTCTGGT ACAGGAAGGC ATAGGACTGC CGAATGCCTT GACCTACGAC
    TCCACCATGC GTCAGGTCTG CTGGGCCGAT GCTGGAACCA AGCGTCTGGA GTGTATTTCA
    CCAAACGGGA CAGGCAGACG CGTGATCCAG AGTAACCTGA ACTACCCTTT CAGCATGGTG
    TACTTCGCCA ATCACTACTA CTACACTGAC TGGAGGAGGG ACGGAGTCAT CTCTCTAGGT
    CGAGATAACC AGTTAACAGA TGAGTACTTA CCTGATCAGA GGTCTCATTT GTATGGTATC
    ACAGTCGCCC CTCCACAATG TTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22351
    Clone ID Related Accession (Same CDS sequence) XM_005169848.2 , XM_005169848.3
    Accession Version XM_005169848.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nidogen-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169848.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCATTCCTGT GGTTGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAGTAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTTCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    GTTCCTGTGG TCGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA ATAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTCCCAAG
    ACCCAGTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAA ACGATGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGACGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGCTCA
    CCCAGAGTAA ACTGTGATCA ACCTGTTCGT CCCAAGACCC AGTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGGAGACGG TGGTGTTCCT GCGGTCGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAGTAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTGATAGT TCCTCCAACG CAGCGTCCGG AGAGTGTCTG TGAGCGCTGG
    AGAGCAAGTC TGTTACAGCA GTATGGAGGT CAGCCAGATT CCCAGCATTA TCTACCTCAG
    TGTGACTCTG CCGGTGAGTT CAACCCAGTT CAGTGTTATG GAGGCAGCAG CTACTGCTGG
    TGTGTGGACG CAAATGGACG AGAAGTACCA GGAACCCGCT CACATGATGC TGTGAAGCCT
    GCCTGTATAC CTACTGTGGC CTCGCCTACG GTGCACCCTC TCCCACGCCC AGATGTGACT
    CCGCCTCCTA CGGGCACGTC TCTTCTCTAC GCCCAGGGCC AGCAGATTGG AGTTTTGCCT
    CTCAATGGCA CACAGATGGA TAAGGAGAGA TCGTCAGTGC TGCTCGCTTT ACATGGCTCG
    ATAGTGGTTG GCATTGATTA CGACTGCAGA GAAAGGAAAG TATACTGGAC AGATCTGGCA
    GGACGGACAA TCAATCGAGC CAGCCTGGAT CCAGGATCAG AACCAGAGAT CATCATCAAT
    ACAGCTCTGA CGAGTCCTGA AGGTCTGGCT GTGGATGTGG CCCGAAGGAG ATTGTTTTGG
    GTGGATAGCA CACTGGACAA GATCGAAACG GCCAATCTCG ATGGAAGTGA CAGACATGTT
    CTGTTCGCCA CAGATTTAGT AAATCCCAGA GCGATGATCG TGGACTCGTC TACAGGAACC
    CTCTACTGGA CTGACTGGAA CCGAGAGGCT CCTAAAATCG AGAGCTCTTC TGTGGATGGG
    CAAAACCGAA GGGTTCTGGT ACAGGAAGGC ATAGGACTGC CGAATGCCTT GACCTACGAC
    TCCACCATGC GTCAGGTCTG CTGGGCCGAT GCTGGAACCA AGCGTCTGGA GTGTATTTCA
    CCAAACGGGA CAGGCAGACG CGTGATCCAG AGTAACCTGA ACTACCCTTT CAGCATGGTG
    TACTTCGCCA ATCACTACTA CTACACTGAC TGGAGGAGGG ACGGAGTCAT CTCTCTAGGT
    CGAGATAACC AGTTAACAGA TGAGTACTTA CCTGATCAGA GGTCTCATTT GTATGGTATC
    ACAGTCGCCC CTCCACAATG TTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169905.1
    CDS31..5217
    Translation

    Target ORF information:

    RefSeq Version XM_005169848.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169848.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCATTCCTGT GGTTGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAGTAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTTCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    GTTCCTGTGG TCGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA ATAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTCCCAAG
    ACCCAGTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAA ACGATGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGACGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGCTCA
    CCCAGAGTAA ACTGTGATCA ACCTGTTCGT CCCAAGACCC AGTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGGAGACGG TGGTGTTCCT GCGGTCGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAGTAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTGATAGT TCCTCCAACG CAGCGTCCGG AGAGTGTCTG TGAGCGCTGG
    AGAGCAAGTC TGTTACAGCA GTATGGAGGT CAGCCAGATT CCCAGCATTA TCTACCTCAG
    TGTGACTCTG CCGGTGAGTT CAACCCAGTT CAGTGTTATG GAGGCAGCAG CTACTGCTGG
    TGTGTGGACG CAAATGGACG AGAAGTACCA GGAACCCGCT CACATGATGC TGTGAAGCCT
    GCCTGTATAC CTACTGTGGC CTCGCCTACG GTGCACCCTC TCCCACGCCC AGATGTGACT
    CCGCCTCCTA CGGGCACGTC TCTTCTCTAC GCCCAGGGCC AGCAGATTGG AGTTTTGCCT
    CTCAATGGCA CACAGATGGA TAAGGAGAGA TCGTCAGTGC TGCTCGCTTT ACATGGCTCG
    ATAGTGGTTG GCATTGATTA CGACTGCAGA GAAAGGAAAG TATACTGGAC AGATCTGGCA
    GGACGGACAA TCAATCGAGC CAGCCTGGAT CCAGGATCAG AACCAGAGAT CATCATCAAT
    ACAGCTCTGA CGAGTCCTGA AGGTCTGGCT GTGGATGTGG CCCGAAGGAG ATTGTTTTGG
    GTGGATAGCA CACTGGACAA GATCGAAACG GCCAATCTCG ATGGAAGTGA CAGACATGTT
    CTGTTCGCCA CAGATTTAGT AAATCCCAGA GCGATGATCG TGGACTCGTC TACAGGAACC
    CTCTACTGGA CTGACTGGAA CCGAGAGGCT CCTAAAATCG AGAGCTCTTC TGTGGATGGG
    CAAAACCGAA GGGTTCTGGT ACAGGAAGGC ATAGGACTGC CGAATGCCTT GACCTACGAC
    TCCACCATGC GTCAGGTCTG CTGGGCCGAT GCTGGAACCA AGCGTCTGGA GTGTATTTCA
    CCAAACGGGA CAGGCAGACG CGTGATCCAG AGTAACCTGA ACTACCCTTT CAGCATGGTG
    TACTTCGCCA ATCACTACTA CTACACTGAC TGGAGGAGGG ACGGAGTCAT CTCTCTAGGT
    CGAGATAACC AGTTAACAGA TGAGTACTTA CCTGATCAGA GGTCTCATTT GTATGGTATC
    ACAGTCGCCC CTCCACAATG TTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22352
    Clone ID Related Accession (Same CDS sequence) XM_005169849.2 , XM_005169849.3
    Accession Version XM_005169849.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nidogen-2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTTCCAAG
    ACCCACTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCCAGAGTAA ACTGTGATCA ACCTGTGATA GTTCCTCCAA CGCAGCGTCC GGAGAGTGTC
    TGTGAGCGCT GGAGAGCAAG TCTGTTACAG CAGTATGGAG GTCAGCCAGA TTCCCAGCAT
    TATCTACCTC AGTGTGACTC TGCCGGTGAG TTCAACCCAG TTCAGTGTTA TGGAGGCAGC
    AGCTACTGCT GGTGTGTGGA CGCAAATGGA CGAGAAGTAC CAGGAACCCG CTCACATGAT
    GCTGTGAAGC CTGCCTGTAT ACCTACTGTG GCCTCGCCTA CGGTGCACCC TCTCCCACGC
    CCAGATGTGA CTCCGCCTCC TACGGGCACG TCTCTTCTCT ACGCCCAGGG CCAGCAGATT
    GGAGTTTTGC CTCTCAATGG CACACAGATG GATAAGGAGA GATCGTCAGT GCTGCTCGCT
    TTACATGGCT CGATAGTGGT TGGCATTGAT TACGACTGCA GAGAAAGGAA AGTATACTGG
    ACAGATCTGG CAGGACGGAC AATCAATCGA GCCAGCCTGG ATCCAGGATC AGAACCAGAG
    ATCATCATCA ATACAGCTCT GACGAGTCCT GAAGGTCTGG CTGTGGATGT GGCCCGAAGG
    AGATTGTTTT GGGTGGATAG CACACTGGAC AAGATCGAAA CGGCCAATCT CGATGGAAGT
    GACAGACATG TTCTGTTCGC CACAGATTTA GTAAATCCCA GAGCGATGAT CGTGGACTCG
    TCTACAGGAA CCCTCTACTG GACTGACTGG AACCGAGAGG CTCCTAAAAT CGAGAGCTCT
    TCTGTGGATG GGCAAAACCG AAGGGTTCTG GTACAGGAAG GCATAGGACT GCCGAATGCC
    TTGACCTACG ACTCCACCAT GCGTCAGGTC TGCTGGGCCG ATGCTGGAAC CAAGCGTCTG
    GAGTGTATTT CACCAAACGG GACAGGCAGA CGCGTGATCC AGAGTAACCT GAACTACCCT
    TTCAGCATGG TGTACTTCGC CAATCACTAC TACTACACTG ACTGGAGGAG GGACGGAGTC
    ATCTCTCTAG GTCGAGATAA CCAGTTAACA GATGAGTACT TACCTGATCA GAGGTCTCAT
    TTGTATGGTA TCACAGTCGC CCCTCCACAA TGTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169906.1
    CDS5..4963
    Translation

    Target ORF information:

    RefSeq Version XM_005169849.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169849.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTTCCAAG
    ACCCACTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCCAGAGTAA ACTGTGATCA ACCTGTGATA GTTCCTCCAA CGCAGCGTCC GGAGAGTGTC
    TGTGAGCGCT GGAGAGCAAG TCTGTTACAG CAGTATGGAG GTCAGCCAGA TTCCCAGCAT
    TATCTACCTC AGTGTGACTC TGCCGGTGAG TTCAACCCAG TTCAGTGTTA TGGAGGCAGC
    AGCTACTGCT GGTGTGTGGA CGCAAATGGA CGAGAAGTAC CAGGAACCCG CTCACATGAT
    GCTGTGAAGC CTGCCTGTAT ACCTACTGTG GCCTCGCCTA CGGTGCACCC TCTCCCACGC
    CCAGATGTGA CTCCGCCTCC TACGGGCACG TCTCTTCTCT ACGCCCAGGG CCAGCAGATT
    GGAGTTTTGC CTCTCAATGG CACACAGATG GATAAGGAGA GATCGTCAGT GCTGCTCGCT
    TTACATGGCT CGATAGTGGT TGGCATTGAT TACGACTGCA GAGAAAGGAA AGTATACTGG
    ACAGATCTGG CAGGACGGAC AATCAATCGA GCCAGCCTGG ATCCAGGATC AGAACCAGAG
    ATCATCATCA ATACAGCTCT GACGAGTCCT GAAGGTCTGG CTGTGGATGT GGCCCGAAGG
    AGATTGTTTT GGGTGGATAG CACACTGGAC AAGATCGAAA CGGCCAATCT CGATGGAAGT
    GACAGACATG TTCTGTTCGC CACAGATTTA GTAAATCCCA GAGCGATGAT CGTGGACTCG
    TCTACAGGAA CCCTCTACTG GACTGACTGG AACCGAGAGG CTCCTAAAAT CGAGAGCTCT
    TCTGTGGATG GGCAAAACCG AAGGGTTCTG GTACAGGAAG GCATAGGACT GCCGAATGCC
    TTGACCTACG ACTCCACCAT GCGTCAGGTC TGCTGGGCCG ATGCTGGAAC CAAGCGTCTG
    GAGTGTATTT CACCAAACGG GACAGGCAGA CGCGTGATCC AGAGTAACCT GAACTACCCT
    TTCAGCATGG TGTACTTCGC CAATCACTAC TACTACACTG ACTGGAGGAG GGACGGAGTC
    ATCTCTCTAG GTCGAGATAA CCAGTTAACA GATGAGTACT TACCTGATCA GAGGTCTCAT
    TTGTATGGTA TCACAGTCGC CCCTCCACAA TGTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22352
    Clone ID Related Accession (Same CDS sequence) XM_005169849.2 , XM_005169849.3
    Accession Version XM_005169849.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nidogen-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169849.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTTCCAAG
    ACCCACTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCCAGAGTAA ACTGTGATCA ACCTGTGATA GTTCCTCCAA CGCAGCGTCC GGAGAGTGTC
    TGTGAGCGCT GGAGAGCAAG TCTGTTACAG CAGTATGGAG GTCAGCCAGA TTCCCAGCAT
    TATCTACCTC AGTGTGACTC TGCCGGTGAG TTCAACCCAG TTCAGTGTTA TGGAGGCAGC
    AGCTACTGCT GGTGTGTGGA CGCAAATGGA CGAGAAGTAC CAGGAACCCG CTCACATGAT
    GCTGTGAAGC CTGCCTGTAT ACCTACTGTG GCCTCGCCTA CGGTGCACCC TCTCCCACGC
    CCAGATGTGA CTCCGCCTCC TACGGGCACG TCTCTTCTCT ACGCCCAGGG CCAGCAGATT
    GGAGTTTTGC CTCTCAATGG CACACAGATG GATAAGGAGA GATCGTCAGT GCTGCTCGCT
    TTACATGGCT CGATAGTGGT TGGCATTGAT TACGACTGCA GAGAAAGGAA AGTATACTGG
    ACAGATCTGG CAGGACGGAC AATCAATCGA GCCAGCCTGG ATCCAGGATC AGAACCAGAG
    ATCATCATCA ATACAGCTCT GACGAGTCCT GAAGGTCTGG CTGTGGATGT GGCCCGAAGG
    AGATTGTTTT GGGTGGATAG CACACTGGAC AAGATCGAAA CGGCCAATCT CGATGGAAGT
    GACAGACATG TTCTGTTCGC CACAGATTTA GTAAATCCCA GAGCGATGAT CGTGGACTCG
    TCTACAGGAA CCCTCTACTG GACTGACTGG AACCGAGAGG CTCCTAAAAT CGAGAGCTCT
    TCTGTGGATG GGCAAAACCG AAGGGTTCTG GTACAGGAAG GCATAGGACT GCCGAATGCC
    TTGACCTACG ACTCCACCAT GCGTCAGGTC TGCTGGGCCG ATGCTGGAAC CAAGCGTCTG
    GAGTGTATTT CACCAAACGG GACAGGCAGA CGCGTGATCC AGAGTAACCT GAACTACCCT
    TTCAGCATGG TGTACTTCGC CAATCACTAC TACTACACTG ACTGGAGGAG GGACGGAGTC
    ATCTCTCTAG GTCGAGATAA CCAGTTAACA GATGAGTACT TACCTGATCA GAGGTCTCAT
    TTGTATGGTA TCACAGTCGC CCCTCCACAA TGTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169906.1
    CDS32..4990
    Translation

    Target ORF information:

    RefSeq Version XM_005169849.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169849.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTTCCAAG
    ACCCACTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGCGT TCCTGTGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAAT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTTC GTCCCAAGAC CCAGTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAAAC GATGGCGTTC CTGTGGTCGG AGCCTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG ACGCAGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCCAGAGTAA ACTGTGATCA ACCTGTGATA GTTCCTCCAA CGCAGCGTCC GGAGAGTGTC
    TGTGAGCGCT GGAGAGCAAG TCTGTTACAG CAGTATGGAG GTCAGCCAGA TTCCCAGCAT
    TATCTACCTC AGTGTGACTC TGCCGGTGAG TTCAACCCAG TTCAGTGTTA TGGAGGCAGC
    AGCTACTGCT GGTGTGTGGA CGCAAATGGA CGAGAAGTAC CAGGAACCCG CTCACATGAT
    GCTGTGAAGC CTGCCTGTAT ACCTACTGTG GCCTCGCCTA CGGTGCACCC TCTCCCACGC
    CCAGATGTGA CTCCGCCTCC TACGGGCACG TCTCTTCTCT ACGCCCAGGG CCAGCAGATT
    GGAGTTTTGC CTCTCAATGG CACACAGATG GATAAGGAGA GATCGTCAGT GCTGCTCGCT
    TTACATGGCT CGATAGTGGT TGGCATTGAT TACGACTGCA GAGAAAGGAA AGTATACTGG
    ACAGATCTGG CAGGACGGAC AATCAATCGA GCCAGCCTGG ATCCAGGATC AGAACCAGAG
    ATCATCATCA ATACAGCTCT GACGAGTCCT GAAGGTCTGG CTGTGGATGT GGCCCGAAGG
    AGATTGTTTT GGGTGGATAG CACACTGGAC AAGATCGAAA CGGCCAATCT CGATGGAAGT
    GACAGACATG TTCTGTTCGC CACAGATTTA GTAAATCCCA GAGCGATGAT CGTGGACTCG
    TCTACAGGAA CCCTCTACTG GACTGACTGG AACCGAGAGG CTCCTAAAAT CGAGAGCTCT
    TCTGTGGATG GGCAAAACCG AAGGGTTCTG GTACAGGAAG GCATAGGACT GCCGAATGCC
    TTGACCTACG ACTCCACCAT GCGTCAGGTC TGCTGGGCCG ATGCTGGAAC CAAGCGTCTG
    GAGTGTATTT CACCAAACGG GACAGGCAGA CGCGTGATCC AGAGTAACCT GAACTACCCT
    TTCAGCATGG TGTACTTCGC CAATCACTAC TACTACACTG ACTGGAGGAG GGACGGAGTC
    ATCTCTCTAG GTCGAGATAA CCAGTTAACA GATGAGTACT TACCTGATCA GAGGTCTCAT
    TTGTATGGTA TCACAGTCGC CCCTCCACAA TGTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22353
    Clone ID Related Accession (Same CDS sequence) XM_005169850.3 , XM_005169850.2
    Accession Version XM_005169850.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nidogen-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169850.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAG TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAAACGATG GCGTTCCTGT GGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGACGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCAGTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AAACGATGGC
    GTTCCTGTGG TCGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGACGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA ATAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG CTCACCCAGA GTAAACTGTG ATCAACCTGT TCGTCCCAAG
    ACCCAGTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGTGT TCCTGCGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAGT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTGA TAGTTCCTCC AACGCAGCGT
    CCGGAGAGTG TCTGTGAGCG CTGGAGAGCA AGTCTGTTAC AGCAGTATGG AGGTCAGCCA
    GATTCCCAGC ATTATCTACC TCAGTGTGAC TCTGCCGGTG AGTTCAACCC AGTTCAGTGT
    TATGGAGGCA GCAGCTACTG CTGGTGTGTG GACGCAAATG GACGAGAAGT ACCAGGAACC
    CGCTCACATG ATGCTGTGAA GCCTGCCTGT ATACCTACTG TGGCCTCGCC TACGGTGCAC
    CCTCTCCCAC GCCCAGATGT GACTCCGCCT CCTACGGGCA CGTCTCTTCT CTACGCCCAG
    GGCCAGCAGA TTGGAGTTTT GCCTCTCAAT GGCACACAGA TGGATAAGGA GAGATCGTCA
    GTGCTGCTCG CTTTACATGG CTCGATAGTG GTTGGCATTG ATTACGACTG CAGAGAAAGG
    AAAGTATACT GGACAGATCT GGCAGGACGG ACAATCAATC GAGCCAGCCT GGATCCAGGA
    TCAGAACCAG AGATCATCAT CAATACAGCT CTGACGAGTC CTGAAGGTCT GGCTGTGGAT
    GTGGCCCGAA GGAGATTGTT TTGGGTGGAT AGCACACTGG ACAAGATCGA AACGGCCAAT
    CTCGATGGAA GTGACAGACA TGTTCTGTTC GCCACAGATT TAGTAAATCC CAGAGCGATG
    ATCGTGGACT CGTCTACAGG AACCCTCTAC TGGACTGACT GGAACCGAGA GGCTCCTAAA
    ATCGAGAGCT CTTCTGTGGA TGGGCAAAAC CGAAGGGTTC TGGTACAGGA AGGCATAGGA
    CTGCCGAATG CCTTGACCTA CGACTCCACC ATGCGTCAGG TCTGCTGGGC CGATGCTGGA
    ACCAAGCGTC TGGAGTGTAT TTCACCAAAC GGGACAGGCA GACGCGTGAT CCAGAGTAAC
    CTGAACTACC CTTTCAGCAT GGTGTACTTC GCCAATCACT ACTACTACAC TGACTGGAGG
    AGGGACGGAG TCATCTCTCT AGGTCGAGAT AACCAGTTAA CAGATGAGTA CTTACCTGAT
    CAGAGGTCTC ATTTGTATGG TATCACAGTC GCCCCTCCAC AATGTTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169907.1
    CDS32..4762
    Translation

    Target ORF information:

    RefSeq Version XM_005169850.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169850.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAG TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAAACGATG GCGTTCCTGT GGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGACGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCAGTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AAACGATGGC
    GTTCCTGTGG TCGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGACGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA ATAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG CTCACCCAGA GTAAACTGTG ATCAACCTGT TCGTCCCAAG
    ACCCAGTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGTGT TCCTGCGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAGT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTGA TAGTTCCTCC AACGCAGCGT
    CCGGAGAGTG TCTGTGAGCG CTGGAGAGCA AGTCTGTTAC AGCAGTATGG AGGTCAGCCA
    GATTCCCAGC ATTATCTACC TCAGTGTGAC TCTGCCGGTG AGTTCAACCC AGTTCAGTGT
    TATGGAGGCA GCAGCTACTG CTGGTGTGTG GACGCAAATG GACGAGAAGT ACCAGGAACC
    CGCTCACATG ATGCTGTGAA GCCTGCCTGT ATACCTACTG TGGCCTCGCC TACGGTGCAC
    CCTCTCCCAC GCCCAGATGT GACTCCGCCT CCTACGGGCA CGTCTCTTCT CTACGCCCAG
    GGCCAGCAGA TTGGAGTTTT GCCTCTCAAT GGCACACAGA TGGATAAGGA GAGATCGTCA
    GTGCTGCTCG CTTTACATGG CTCGATAGTG GTTGGCATTG ATTACGACTG CAGAGAAAGG
    AAAGTATACT GGACAGATCT GGCAGGACGG ACAATCAATC GAGCCAGCCT GGATCCAGGA
    TCAGAACCAG AGATCATCAT CAATACAGCT CTGACGAGTC CTGAAGGTCT GGCTGTGGAT
    GTGGCCCGAA GGAGATTGTT TTGGGTGGAT AGCACACTGG ACAAGATCGA AACGGCCAAT
    CTCGATGGAA GTGACAGACA TGTTCTGTTC GCCACAGATT TAGTAAATCC CAGAGCGATG
    ATCGTGGACT CGTCTACAGG AACCCTCTAC TGGACTGACT GGAACCGAGA GGCTCCTAAA
    ATCGAGAGCT CTTCTGTGGA TGGGCAAAAC CGAAGGGTTC TGGTACAGGA AGGCATAGGA
    CTGCCGAATG CCTTGACCTA CGACTCCACC ATGCGTCAGG TCTGCTGGGC CGATGCTGGA
    ACCAAGCGTC TGGAGTGTAT TTCACCAAAC GGGACAGGCA GACGCGTGAT CCAGAGTAAC
    CTGAACTACC CTTTCAGCAT GGTGTACTTC GCCAATCACT ACTACTACAC TGACTGGAGG
    AGGGACGGAG TCATCTCTCT AGGTCGAGAT AACCAGTTAA CAGATGAGTA CTTACCTGAT
    CAGAGGTCTC ATTTGTATGG TATCACAGTC GCCCCTCCAC AATGTTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22353
    Clone ID Related Accession (Same CDS sequence) XM_005169850.3 , XM_005169850.2
    Accession Version XM_005169850.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nidogen-2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAG TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAAACGATG GCGTTCCTGT GGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGACGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCAGTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AAACGATGGC
    GTTCCTGTGG TCGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGACGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA ATAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG CTCACCCAGA GTAAACTGTG ATCAACCTGT TCGTCCCAAG
    ACCCAGTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGTGT TCCTGCGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAGT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTGA TAGTTCCTCC AACGCAGCGT
    CCGGAGAGTG TCTGTGAGCG CTGGAGAGCA AGTCTGTTAC AGCAGTATGG AGGTCAGCCA
    GATTCCCAGC ATTATCTACC TCAGTGTGAC TCTGCCGGTG AGTTCAACCC AGTTCAGTGT
    TATGGAGGCA GCAGCTACTG CTGGTGTGTG GACGCAAATG GACGAGAAGT ACCAGGAACC
    CGCTCACATG ATGCTGTGAA GCCTGCCTGT ATACCTACTG TGGCCTCGCC TACGGTGCAC
    CCTCTCCCAC GCCCAGATGT GACTCCGCCT CCTACGGGCA CGTCTCTTCT CTACGCCCAG
    GGCCAGCAGA TTGGAGTTTT GCCTCTCAAT GGCACACAGA TGGATAAGGA GAGATCGTCA
    GTGCTGCTCG CTTTACATGG CTCGATAGTG GTTGGCATTG ATTACGACTG CAGAGAAAGG
    AAAGTATACT GGACAGATCT GGCAGGACGG ACAATCAATC GAGCCAGCCT GGATCCAGGA
    TCAGAACCAG AGATCATCAT CAATACAGCT CTGACGAGTC CTGAAGGTCT GGCTGTGGAT
    GTGGCCCGAA GGAGATTGTT TTGGGTGGAT AGCACACTGG ACAAGATCGA AACGGCCAAT
    CTCGATGGAA GTGACAGACA TGTTCTGTTC GCCACAGATT TAGTAAATCC CAGAGCGATG
    ATCGTGGACT CGTCTACAGG AACCCTCTAC TGGACTGACT GGAACCGAGA GGCTCCTAAA
    ATCGAGAGCT CTTCTGTGGA TGGGCAAAAC CGAAGGGTTC TGGTACAGGA AGGCATAGGA
    CTGCCGAATG CCTTGACCTA CGACTCCACC ATGCGTCAGG TCTGCTGGGC CGATGCTGGA
    ACCAAGCGTC TGGAGTGTAT TTCACCAAAC GGGACAGGCA GACGCGTGAT CCAGAGTAAC
    CTGAACTACC CTTTCAGCAT GGTGTACTTC GCCAATCACT ACTACTACAC TGACTGGAGG
    AGGGACGGAG TCATCTCTCT AGGTCGAGAT AACCAGTTAA CAGATGAGTA CTTACCTGAT
    CAGAGGTCTC ATTTGTATGG TATCACAGTC GCCCCTCCAC AATGTTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169907.1
    CDS5..4735
    Translation

    Target ORF information:

    RefSeq Version XM_005169850.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169850.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAG TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAAACGATG GCGTTCCTGT GGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGACGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCAGTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AAACGATGGC
    GTTCCTGTGG TCGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGACGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA ATAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG CTCACCCAGA GTAAACTGTG ATCAACCTGT TCGTCCCAAG
    ACCCAGTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGTGT TCCTGCGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAGT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTGA TAGTTCCTCC AACGCAGCGT
    CCGGAGAGTG TCTGTGAGCG CTGGAGAGCA AGTCTGTTAC AGCAGTATGG AGGTCAGCCA
    GATTCCCAGC ATTATCTACC TCAGTGTGAC TCTGCCGGTG AGTTCAACCC AGTTCAGTGT
    TATGGAGGCA GCAGCTACTG CTGGTGTGTG GACGCAAATG GACGAGAAGT ACCAGGAACC
    CGCTCACATG ATGCTGTGAA GCCTGCCTGT ATACCTACTG TGGCCTCGCC TACGGTGCAC
    CCTCTCCCAC GCCCAGATGT GACTCCGCCT CCTACGGGCA CGTCTCTTCT CTACGCCCAG
    GGCCAGCAGA TTGGAGTTTT GCCTCTCAAT GGCACACAGA TGGATAAGGA GAGATCGTCA
    GTGCTGCTCG CTTTACATGG CTCGATAGTG GTTGGCATTG ATTACGACTG CAGAGAAAGG
    AAAGTATACT GGACAGATCT GGCAGGACGG ACAATCAATC GAGCCAGCCT GGATCCAGGA
    TCAGAACCAG AGATCATCAT CAATACAGCT CTGACGAGTC CTGAAGGTCT GGCTGTGGAT
    GTGGCCCGAA GGAGATTGTT TTGGGTGGAT AGCACACTGG ACAAGATCGA AACGGCCAAT
    CTCGATGGAA GTGACAGACA TGTTCTGTTC GCCACAGATT TAGTAAATCC CAGAGCGATG
    ATCGTGGACT CGTCTACAGG AACCCTCTAC TGGACTGACT GGAACCGAGA GGCTCCTAAA
    ATCGAGAGCT CTTCTGTGGA TGGGCAAAAC CGAAGGGTTC TGGTACAGGA AGGCATAGGA
    CTGCCGAATG CCTTGACCTA CGACTCCACC ATGCGTCAGG TCTGCTGGGC CGATGCTGGA
    ACCAAGCGTC TGGAGTGTAT TTCACCAAAC GGGACAGGCA GACGCGTGAT CCAGAGTAAC
    CTGAACTACC CTTTCAGCAT GGTGTACTTC GCCAATCACT ACTACTACAC TGACTGGAGG
    AGGGACGGAG TCATCTCTCT AGGTCGAGAT AACCAGTTAA CAGATGAGTA CTTACCTGAT
    CAGAGGTCTC ATTTGTATGG TATCACAGTC GCCCCTCCAC AATGTTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22355
    Clone ID Related Accession (Same CDS sequence) XM_005169852.2 , XM_005169852.3
    Accession Version XM_005169852.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nidogen-2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAG TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAAACGATG GCGTTCCTGT GGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGACGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTGATAGTTC CTCCAACGCA GCGTCCGGAG AGTGTCTGTG AGCGCTGGAG AGCAAGTCTG
    TTACAGCAGT ATGGAGGTCA GCCAGATTCC CAGCATTATC TACCTCAGTG TGACTCTGCC
    GGTGAGTTCA ACCCAGTTCA GTGTTATGGA GGCAGCAGCT ACTGCTGGTG TGTGGACGCA
    AATGGACGAG AAGTACCAGG AACCCGCTCA CATGATGCTG TGAAGCCTGC CTGTATACCT
    ACTGTGGCCT CGCCTACGGT GCACCCTCTC CCACGCCCAG ATGTGACTCC GCCTCCTACG
    GGCACGTCTC TTCTCTACGC CCAGGGCCAG CAGATTGGAG TTTTGCCTCT CAATGGCACA
    CAGATGGATA AGGAGAGATC GTCAGTGCTG CTCGCTTTAC ATGGCTCGAT AGTGGTTGGC
    ATTGATTACG ACTGCAGAGA AAGGAAAGTA TACTGGACAG ATCTGGCAGG ACGGACAATC
    AATCGAGCCA GCCTGGATCC AGGATCAGAA CCAGAGATCA TCATCAATAC AGCTCTGACG
    AGTCCTGAAG GTCTGGCTGT GGATGTGGCC CGAAGGAGAT TGTTTTGGGT GGATAGCACA
    CTGGACAAGA TCGAAACGGC CAATCTCGAT GGAAGTGACA GACATGTTCT GTTCGCCACA
    GATTTAGTAA ATCCCAGAGC GATGATCGTG GACTCGTCTA CAGGAACCCT CTACTGGACT
    GACTGGAACC GAGAGGCTCC TAAAATCGAG AGCTCTTCTG TGGATGGGCA AAACCGAAGG
    GTTCTGGTAC AGGAAGGCAT AGGACTGCCG AATGCCTTGA CCTACGACTC CACCATGCGT
    CAGGTCTGCT GGGCCGATGC TGGAACCAAG CGTCTGGAGT GTATTTCACC AAACGGGACA
    GGCAGACGCG TGATCCAGAG TAACCTGAAC TACCCTTTCA GCATGGTGTA CTTCGCCAAT
    CACTACTACT ACACTGACTG GAGGAGGGAC GGAGTCATCT CTCTAGGTCG AGATAACCAG
    TTAACAGATG AGTACTTACC TGATCAGAGG TCTCATTTGT ATGGTATCAC AGTCGCCCCT
    CCACAATGTT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169909.1
    CDS5..4279
    Translation

    Target ORF information:

    RefSeq Version XM_005169852.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169852.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAG TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAAACGATG GCGTTCCTGT GGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGACGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTGATAGTTC CTCCAACGCA GCGTCCGGAG AGTGTCTGTG AGCGCTGGAG AGCAAGTCTG
    TTACAGCAGT ATGGAGGTCA GCCAGATTCC CAGCATTATC TACCTCAGTG TGACTCTGCC
    GGTGAGTTCA ACCCAGTTCA GTGTTATGGA GGCAGCAGCT ACTGCTGGTG TGTGGACGCA
    AATGGACGAG AAGTACCAGG AACCCGCTCA CATGATGCTG TGAAGCCTGC CTGTATACCT
    ACTGTGGCCT CGCCTACGGT GCACCCTCTC CCACGCCCAG ATGTGACTCC GCCTCCTACG
    GGCACGTCTC TTCTCTACGC CCAGGGCCAG CAGATTGGAG TTTTGCCTCT CAATGGCACA
    CAGATGGATA AGGAGAGATC GTCAGTGCTG CTCGCTTTAC ATGGCTCGAT AGTGGTTGGC
    ATTGATTACG ACTGCAGAGA AAGGAAAGTA TACTGGACAG ATCTGGCAGG ACGGACAATC
    AATCGAGCCA GCCTGGATCC AGGATCAGAA CCAGAGATCA TCATCAATAC AGCTCTGACG
    AGTCCTGAAG GTCTGGCTGT GGATGTGGCC CGAAGGAGAT TGTTTTGGGT GGATAGCACA
    CTGGACAAGA TCGAAACGGC CAATCTCGAT GGAAGTGACA GACATGTTCT GTTCGCCACA
    GATTTAGTAA ATCCCAGAGC GATGATCGTG GACTCGTCTA CAGGAACCCT CTACTGGACT
    GACTGGAACC GAGAGGCTCC TAAAATCGAG AGCTCTTCTG TGGATGGGCA AAACCGAAGG
    GTTCTGGTAC AGGAAGGCAT AGGACTGCCG AATGCCTTGA CCTACGACTC CACCATGCGT
    CAGGTCTGCT GGGCCGATGC TGGAACCAAG CGTCTGGAGT GTATTTCACC AAACGGGACA
    GGCAGACGCG TGATCCAGAG TAACCTGAAC TACCCTTTCA GCATGGTGTA CTTCGCCAAT
    CACTACTACT ACACTGACTG GAGGAGGGAC GGAGTCATCT CTCTAGGTCG AGATAACCAG
    TTAACAGATG AGTACTTACC TGATCAGAGG TCTCATTTGT ATGGTATCAC AGTCGCCCCT
    CCACAATGTT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22355
    Clone ID Related Accession (Same CDS sequence) XM_005169852.2 , XM_005169852.3
    Accession Version XM_005169852.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nidogen-2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169852.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAG TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAAACGATG GCGTTCCTGT GGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGACGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTGATAGTTC CTCCAACGCA GCGTCCGGAG AGTGTCTGTG AGCGCTGGAG AGCAAGTCTG
    TTACAGCAGT ATGGAGGTCA GCCAGATTCC CAGCATTATC TACCTCAGTG TGACTCTGCC
    GGTGAGTTCA ACCCAGTTCA GTGTTATGGA GGCAGCAGCT ACTGCTGGTG TGTGGACGCA
    AATGGACGAG AAGTACCAGG AACCCGCTCA CATGATGCTG TGAAGCCTGC CTGTATACCT
    ACTGTGGCCT CGCCTACGGT GCACCCTCTC CCACGCCCAG ATGTGACTCC GCCTCCTACG
    GGCACGTCTC TTCTCTACGC CCAGGGCCAG CAGATTGGAG TTTTGCCTCT CAATGGCACA
    CAGATGGATA AGGAGAGATC GTCAGTGCTG CTCGCTTTAC ATGGCTCGAT AGTGGTTGGC
    ATTGATTACG ACTGCAGAGA AAGGAAAGTA TACTGGACAG ATCTGGCAGG ACGGACAATC
    AATCGAGCCA GCCTGGATCC AGGATCAGAA CCAGAGATCA TCATCAATAC AGCTCTGACG
    AGTCCTGAAG GTCTGGCTGT GGATGTGGCC CGAAGGAGAT TGTTTTGGGT GGATAGCACA
    CTGGACAAGA TCGAAACGGC CAATCTCGAT GGAAGTGACA GACATGTTCT GTTCGCCACA
    GATTTAGTAA ATCCCAGAGC GATGATCGTG GACTCGTCTA CAGGAACCCT CTACTGGACT
    GACTGGAACC GAGAGGCTCC TAAAATCGAG AGCTCTTCTG TGGATGGGCA AAACCGAAGG
    GTTCTGGTAC AGGAAGGCAT AGGACTGCCG AATGCCTTGA CCTACGACTC CACCATGCGT
    CAGGTCTGCT GGGCCGATGC TGGAACCAAG CGTCTGGAGT GTATTTCACC AAACGGGACA
    GGCAGACGCG TGATCCAGAG TAACCTGAAC TACCCTTTCA GCATGGTGTA CTTCGCCAAT
    CACTACTACT ACACTGACTG GAGGAGGGAC GGAGTCATCT CTCTAGGTCG AGATAACCAG
    TTAACAGATG AGTACTTACC TGATCAGAGG TCTCATTTGT ATGGTATCAC AGTCGCCCCT
    CCACAATGTT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169909.1
    CDS32..4306
    Translation

    Target ORF information:

    RefSeq Version XM_005169852.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169852.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCGTCC CAAGACCCAG TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAAACGATG GCGTTCCTGT GGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGACGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTGATAGTTC CTCCAACGCA GCGTCCGGAG AGTGTCTGTG AGCGCTGGAG AGCAAGTCTG
    TTACAGCAGT ATGGAGGTCA GCCAGATTCC CAGCATTATC TACCTCAGTG TGACTCTGCC
    GGTGAGTTCA ACCCAGTTCA GTGTTATGGA GGCAGCAGCT ACTGCTGGTG TGTGGACGCA
    AATGGACGAG AAGTACCAGG AACCCGCTCA CATGATGCTG TGAAGCCTGC CTGTATACCT
    ACTGTGGCCT CGCCTACGGT GCACCCTCTC CCACGCCCAG ATGTGACTCC GCCTCCTACG
    GGCACGTCTC TTCTCTACGC CCAGGGCCAG CAGATTGGAG TTTTGCCTCT CAATGGCACA
    CAGATGGATA AGGAGAGATC GTCAGTGCTG CTCGCTTTAC ATGGCTCGAT AGTGGTTGGC
    ATTGATTACG ACTGCAGAGA AAGGAAAGTA TACTGGACAG ATCTGGCAGG ACGGACAATC
    AATCGAGCCA GCCTGGATCC AGGATCAGAA CCAGAGATCA TCATCAATAC AGCTCTGACG
    AGTCCTGAAG GTCTGGCTGT GGATGTGGCC CGAAGGAGAT TGTTTTGGGT GGATAGCACA
    CTGGACAAGA TCGAAACGGC CAATCTCGAT GGAAGTGACA GACATGTTCT GTTCGCCACA
    GATTTAGTAA ATCCCAGAGC GATGATCGTG GACTCGTCTA CAGGAACCCT CTACTGGACT
    GACTGGAACC GAGAGGCTCC TAAAATCGAG AGCTCTTCTG TGGATGGGCA AAACCGAAGG
    GTTCTGGTAC AGGAAGGCAT AGGACTGCCG AATGCCTTGA CCTACGACTC CACCATGCGT
    CAGGTCTGCT GGGCCGATGC TGGAACCAAG CGTCTGGAGT GTATTTCACC AAACGGGACA
    GGCAGACGCG TGATCCAGAG TAACCTGAAC TACCCTTTCA GCATGGTGTA CTTCGCCAAT
    CACTACTACT ACACTGACTG GAGGAGGGAC GGAGTCATCT CTCTAGGTCG AGATAACCAG
    TTAACAGATG AGTACTTACC TGATCAGAGG TCTCATTTGT ATGGTATCAC AGTCGCCCCT
    CCACAATGTT TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55694
    Clone ID Related Accession (Same CDS sequence) XM_017351774.1 , XM_017351774.2
    Accession Version XM_017351774.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nidogen-2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTCCCAAG
    ACCCAGTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGTGT TCCTGCGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAGT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTGA TAGTTCCTCC AACGCAGCGT
    CCGGAGAGTG TCTGTGAGCG CTGGAGAGCA AGTCTGTTAC AGCAGTATGG AGGTCAGCCA
    GATTCCCAGC ATTATCTACC TCAGTGTGAC TCTGCCGGTG AGTTCAACCC AGTTCAGTGT
    TATGGAGGCA GCAGCTACTG CTGGTGTGTG GACGCAAATG GACGAGAAGT ACCAGGAACC
    CGCTCACATG ATGCTGTGAA GCCTGCCTGT ATACCTACTG TGGCCTCGCC TACGGTGCAC
    CCTCTCCCAC GCCCAGATGT GACTCCGCCT CCTACGGGCA CGTCTCTTCT CTACGCCCAG
    GGCCAGCAGA TTGGAGTTTT GCCTCTCAAT GGCACACAGA TGGATAAGGA GAGATCGTCA
    GTGCTGCTCG CTTTACATGG CTCGATAGTG GTTGGCATTG ATTACGACTG CAGAGAAAGG
    AAAGTATACT GGACAGATCT GGCAGGACGG ACAATCAATC GAGCCAGCCT GGATCCAGGA
    TCAGAACCAG AGATCATCAT CAATACAGCT CTGACGAGTC CTGAAGGTCT GGCTGTGGAT
    GTGGCCCGAA GGAGATTGTT TTGGGTGGAT AGCACACTGG ACAAGATCGA AACGGCCAAT
    CTCGATGGAA GTGACAGACA TGTTCTGTTC GCCACAGATT TAGTAAATCC CAGAGCGATG
    ATCGTGGACT CGTCTACAGG AACCCTCTAC TGGACTGACT GGAACCGAGA GGCTCCTAAA
    ATCGAGAGCT CTTCTGTGGA TGGGCAAAAC CGAAGGGTTC TGGTACAGGA AGGCATAGGA
    CTGCCGAATG CCTTGACCTA CGACTCCACC ATGCGTCAGG TCTGCTGGGC CGATGCTGGA
    ACCAAGCGTC TGGAGTGTAT TTCACCAAAC GGGACAGGCA GACGCGTGAT CCAGAGTAAC
    CTGAACTACC CTTTCAGCAT GGTGTACTTC GCCAATCACT ACTACTACAC TGACTGGAGG
    AGGGACGGAG TCATCTCTCT AGGTCGAGAT AACCAGTTAA CAGATGAGTA CTTACCTGAT
    CAGAGGTCTC ATTTGTATGG TATCACAGTC GCCCCTCCAC AATGTTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207263.1
    CDS5..4735
    Translation

    Target ORF information:

    RefSeq Version XM_017351774.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351774.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTCCCAAG
    ACCCAGTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGTGT TCCTGCGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAGT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTGA TAGTTCCTCC AACGCAGCGT
    CCGGAGAGTG TCTGTGAGCG CTGGAGAGCA AGTCTGTTAC AGCAGTATGG AGGTCAGCCA
    GATTCCCAGC ATTATCTACC TCAGTGTGAC TCTGCCGGTG AGTTCAACCC AGTTCAGTGT
    TATGGAGGCA GCAGCTACTG CTGGTGTGTG GACGCAAATG GACGAGAAGT ACCAGGAACC
    CGCTCACATG ATGCTGTGAA GCCTGCCTGT ATACCTACTG TGGCCTCGCC TACGGTGCAC
    CCTCTCCCAC GCCCAGATGT GACTCCGCCT CCTACGGGCA CGTCTCTTCT CTACGCCCAG
    GGCCAGCAGA TTGGAGTTTT GCCTCTCAAT GGCACACAGA TGGATAAGGA GAGATCGTCA
    GTGCTGCTCG CTTTACATGG CTCGATAGTG GTTGGCATTG ATTACGACTG CAGAGAAAGG
    AAAGTATACT GGACAGATCT GGCAGGACGG ACAATCAATC GAGCCAGCCT GGATCCAGGA
    TCAGAACCAG AGATCATCAT CAATACAGCT CTGACGAGTC CTGAAGGTCT GGCTGTGGAT
    GTGGCCCGAA GGAGATTGTT TTGGGTGGAT AGCACACTGG ACAAGATCGA AACGGCCAAT
    CTCGATGGAA GTGACAGACA TGTTCTGTTC GCCACAGATT TAGTAAATCC CAGAGCGATG
    ATCGTGGACT CGTCTACAGG AACCCTCTAC TGGACTGACT GGAACCGAGA GGCTCCTAAA
    ATCGAGAGCT CTTCTGTGGA TGGGCAAAAC CGAAGGGTTC TGGTACAGGA AGGCATAGGA
    CTGCCGAATG CCTTGACCTA CGACTCCACC ATGCGTCAGG TCTGCTGGGC CGATGCTGGA
    ACCAAGCGTC TGGAGTGTAT TTCACCAAAC GGGACAGGCA GACGCGTGAT CCAGAGTAAC
    CTGAACTACC CTTTCAGCAT GGTGTACTTC GCCAATCACT ACTACTACAC TGACTGGAGG
    AGGGACGGAG TCATCTCTCT AGGTCGAGAT AACCAGTTAA CAGATGAGTA CTTACCTGAT
    CAGAGGTCTC ATTTGTATGG TATCACAGTC GCCCCTCCAC AATGTTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55694
    Clone ID Related Accession (Same CDS sequence) XM_017351774.1 , XM_017351774.2
    Accession Version XM_017351774.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nidogen-2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTCCCAAG
    ACCCAGTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGTGT TCCTGCGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAGT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTGA TAGTTCCTCC AACGCAGCGT
    CCGGAGAGTG TCTGTGAGCG CTGGAGAGCA AGTCTGTTAC AGCAGTATGG AGGTCAGCCA
    GATTCCCAGC ATTATCTACC TCAGTGTGAC TCTGCCGGTG AGTTCAACCC AGTTCAGTGT
    TATGGAGGCA GCAGCTACTG CTGGTGTGTG GACGCAAATG GACGAGAAGT ACCAGGAACC
    CGCTCACATG ATGCTGTGAA GCCTGCCTGT ATACCTACTG TGGCCTCGCC TACGGTGCAC
    CCTCTCCCAC GCCCAGATGT GACTCCGCCT CCTACGGGCA CGTCTCTTCT CTACGCCCAG
    GGCCAGCAGA TTGGAGTTTT GCCTCTCAAT GGCACACAGA TGGATAAGGA GAGATCGTCA
    GTGCTGCTCG CTTTACATGG CTCGATAGTG GTTGGCATTG ATTACGACTG CAGAGAAAGG
    AAAGTATACT GGACAGATCT GGCAGGACGG ACAATCAATC GAGCCAGCCT GGATCCAGGA
    TCAGAACCAG AGATCATCAT CAATACAGCT CTGACGAGTC CTGAAGGTCT GGCTGTGGAT
    GTGGCCCGAA GGAGATTGTT TTGGGTGGAT AGCACACTGG ACAAGATCGA AACGGCCAAT
    CTCGATGGAA GTGACAGACA TGTTCTGTTC GCCACAGATT TAGTAAATCC CAGAGCGATG
    ATCGTGGACT CGTCTACAGG AACCCTCTAC TGGACTGACT GGAACCGAGA GGCTCCTAAA
    ATCGAGAGCT CTTCTGTGGA TGGGCAAAAC CGAAGGGTTC TGGTACAGGA AGGCATAGGA
    CTGCCGAATG CCTTGACCTA CGACTCCACC ATGCGTCAGG TCTGCTGGGC CGATGCTGGA
    ACCAAGCGTC TGGAGTGTAT TTCACCAAAC GGGACAGGCA GACGCGTGAT CCAGAGTAAC
    CTGAACTACC CTTTCAGCAT GGTGTACTTC GCCAATCACT ACTACTACAC TGACTGGAGG
    AGGGACGGAG TCATCTCTCT AGGTCGAGAT AACCAGTTAA CAGATGAGTA CTTACCTGAT
    CAGAGGTCTC ATTTGTATGG TATCACAGTC GCCCCTCCAC AATGTTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207263.1
    CDS32..4762
    Translation

    Target ORF information:

    RefSeq Version XM_017351774.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio wu:fb77a09 (wu:fb77a09), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351774.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCTCACGA GCTGCATGTG TACCAGCTGC TCGCCAAAAA TGACCCGGGA CAAAATAGCG 
    CTGCTCTTTT TACAGCTTTC ATGGGCATTT CATCATGTCG CAGGGATTGA CAGGCATGAC
    ATCTATCCGT ACGGGATGTT TTACGGCGAT GTGACTCTGC AAGAGGGAGA CGATGAGACC
    TCCAAAGTGA TCACGCTCAG CAAACCCATG CACTTCTATG AGGCGCCCTT CACTAATTTA
    TACGTGACCA CCAATGGAAT CATCTCAACT CAGGATCTTC CCATGGAGAA GCAGTATGTG
    GACGACGGCT TTCCCACCGA CTTCCCCGTC ATCGCCCCCT TTCTGGCCGA CATTGACACC
    AGCAAAGGAA AAGGATCCAT CTACTACCGG CAGACGGAGT CTCCCACCGT GCTGAAGCGT
    GCAGAAGCGG ATGTCAAAAG AGGCTTTCCA GATGCCACGT TCAGCCCTAC CCACGCCTTT
    ATCGCCACCT GGGAGAATGT GTCAGCTTAT GAAGAGGTCG CGCGCAGCTC TAGCCCATCC
    CATCGGGTAA ACACGTTCCA AGTGGTGCTT GCGTACAATG AGAAGGACAC TTATGCGCTC
    TTCCTATACC CTGAGGATGG CCTGCAGTTT TTCGGGACAC GGCCCAAAGA ATCTTACAAT
    GTTGAAATTG AGCTTCCAGC TCGTGTGGGA TTTTCCAGAG GAGAGATTTC CTACTTGATT
    TTTTCTCGAA CAGAAGGGCC GTATTATAGC CTCACCAGCA ATGAGCAGTC CGTCAAGAAC
    CTGTACCAAA AGGGAAACAT AGGTGAGCCC GGAGTTTGGC TCTTCCATGT TGGAAACCTT
    TATTCCTTCC AGAATGTGGT TCCTGCTCAG CAAGAGGCCG TTTCAAGCAA ATCTCCCACT
    GTCCAGGACA CTGTGTTCAC TGATGAAGAC TACACAGACG AGGAGGAGTA CGATCCCATC
    GACCCAGACT TCCAGGATCC CACAACAACA GAGTTTCTGG AGCCGGAGCC AGACTCCTCT
    CTGTTGGAGC GTCTCAACCA GGAGACTCCC CTGGGTCAGT CTCCATTGCA GCCTTCAGTG
    TCGGGTCCAG AAGATCTTCC TCAGCCGGAT CCTCATCATG TCCAGCCTGA GGACAACACT
    CAGCCCCAGT GGCCCTTACC AAAACATGCC ATTCTGCAGG TGTACCCGAA CCGCGTGAAG
    GATGTTCCAA CTCACCCTTC TGATGGCCAA GTGTTCAGTG TTGAGGAGAA AGTGGACTTT
    GACTCTGGAG TGATCCATTA CTCGACAGAT AATAAAGAGA CGTGTGAGCG TTTCCAGCAG
    CAGTGCAGTC TAAACGCTCA CTGTACAGAT CATTCCACCG GCTTCTGCTG TCACTGCAAC
    TCCGGCTTTT ACGGCAACGG CCGCCACTGC CTGCCAAACG GTGCACCTCA GCGTGTGAAT
    GGAAAGGTCA GAGGGACTGT TCTTGTTGGT GGGACAGCAG TGGATCTGGG AAACATCGAC
    CTGCATGCCT ACATCGTGGT GGGCGATGGA CGAGCATACA CCGCCATCAG TGAGGTGCCG
    GAGCCGGCGG GCTGGGCCCT CATGCCCGTG GCCCCCATCG GAGGTCTATT TGGTTGGCTC
    TTCGCCCTGG AGCTGCCAAA CAGCCTTAAT GGCTTCAGTA TCACCGGTGC TGAATTCACT
    CGGCGTGCTG ATGTGACCTT CTCTCCCGGT AACCAGCGTC TCAGCATCGT CCAGACAGCC
    ACGGGTTTGG ATAACCAGAA CTACCTCAAT GTGGACACTC ATCTACAGGG CAGCGTTCCC
    TTCATCCCTC CTGGTGCCAC AGTGCAGATG GAGCCATTTA AGGACACCTA CCAGTATTAT
    CCTTCATTGA TTACTTCCAC ATCGGTGCGT GAGTTCAAAG TCGTGTCAGC TGAAAACGGA
    GCAGAAACTT TCACTTTCCA AGTTAAGCAG AACATCACAT ACAGGGACTG CAGTCACAGG
    CCCCGCAGCA GACTGGAGAC ACTGGAGCTG AAGATGGAGC GCGTATTTGT TATGTACGTC
    AGAGAGGAGC GCATCCTTCG ATATGCCATC ACCAACAAGA TCGGACCACT AGGAGCTGTA
    GATCCCGAGC CAGAAAACGT AAACCCCTGT TACTCTGGAA ATCACGACTG TGATACAACG
    GCTCGATGTG TGCCAGGTGA AGGACAGCTG TTTTCTTGCC AATGTGCCAC AGGTTACAGA
    GGAGACGGAC GCAACTGTTA TGATGTTGAT GAGTGTGCAG AAGGTCTGAG CTCTTGTGGT
    GCTCACTCTC ACTGTGTGAA TCTGCCTGGA AGCCATCGCT GTCAGTGTGA GAGCGGATTC
    CAGTTTGGAT TTGATGGACG CACATGTCAG GATGTGGACG AGTGTGGTGA GCAGCCGTGT
    CACGCCCAGG CCTTGTGCTC AAACAGTCCG GGATCTTTCC GGTGCCAGTG CCAACCGGGA
    TACCATGGAG ATGGTTTCCA GTGCCACCCT CAAAATGGTC ATCCCAAGAC CCGCTGTGAG
    CAGCACAGAG ACAGTCTTCA GAGTGGAGAT GGTGGCGTTC CTGCCGTCGG AGCTTTCATC
    CCTCAGTGTG ATGAGGAGGG TCAGTACAGG CCGCTGCAGT GCCACGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAATAG AGGGCAGGAG AGAGCAGGAA CCAGAACTCC ACCCGGATCA
    CCACCTAGAA ACTGCGATGA ACCTGTTCGT CCCAAGACCC ACTGTGAGCA GCACAGAGAC
    AGTCTTCAGA GTGAAAACGG GGGCGTTCCT GTGGTTGGAG CCTTCATCCC TCAGTGTGAT
    GAGGAGGGTC AGTACAGGCC GCAGCAGTGC CACGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAATAGAG GGCAGGAGAG AGCAGGAACC AGAACTCCAC CCGGATCACC CAGAGTAAAC
    TGTGATCAAC CTGTTCATCT CAGGACCCGC TGTGAGCAGC ACAGAGACAG TCTTCAGAGT
    GGAGACGGTG GCGTTCCTGC AGTCGGAGCC TTCATCCCTC AGTGTGATGA GGAGGGTCAG
    TACAGGCCGC AGCAGTGCCA CGGCTCCACT GGACACTGCT GGTGTGTGGA CAATAGAGGG
    CAGGAGAGAG CAGGAACCAG AACTCCACCC GGATCACCCA GAGTAAACTG TGATCAACCT
    GTTCGTCCCA AGACCCACTG TGAGCAGCAC AGAGACAGTC TTCAGAGTGG AGACGGTGGC
    ATTCCTGTGG TTGGAGCCTT CATCCCTCAG TGTGATGAGG AGGGTCAGTA CAGGCCGCAG
    CAGTGCCACG GCTCCACTGG ACACTGCTGG TGTGTGGACA GTAGAGGGCA GGAGAGAGCA
    GGAACCAGAA CTCCACCCGG ATCACCCAGA GTAAACTGTG ATCAACCTGT TCGTCCCAAG
    ACCCAGTGTG AGCAGCACAG AGACAGTCTT CAGAGTGGAG ACGGTGGTGT TCCTGCGGTC
    GGAGCCTTCA TCCCTCAGTG TGATGAGGAG GGTCAGTACA GGCCGCAGCA GTGCCACGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAGT AGAGGGCAGG AGAGAGCAGG AACCAGAACT
    CCACCCGGAT CACCCAGAGT AAACTGTGAT CAACCTGTGA TAGTTCCTCC AACGCAGCGT
    CCGGAGAGTG TCTGTGAGCG CTGGAGAGCA AGTCTGTTAC AGCAGTATGG AGGTCAGCCA
    GATTCCCAGC ATTATCTACC TCAGTGTGAC TCTGCCGGTG AGTTCAACCC AGTTCAGTGT
    TATGGAGGCA GCAGCTACTG CTGGTGTGTG GACGCAAATG GACGAGAAGT ACCAGGAACC
    CGCTCACATG ATGCTGTGAA GCCTGCCTGT ATACCTACTG TGGCCTCGCC TACGGTGCAC
    CCTCTCCCAC GCCCAGATGT GACTCCGCCT CCTACGGGCA CGTCTCTTCT CTACGCCCAG
    GGCCAGCAGA TTGGAGTTTT GCCTCTCAAT GGCACACAGA TGGATAAGGA GAGATCGTCA
    GTGCTGCTCG CTTTACATGG CTCGATAGTG GTTGGCATTG ATTACGACTG CAGAGAAAGG
    AAAGTATACT GGACAGATCT GGCAGGACGG ACAATCAATC GAGCCAGCCT GGATCCAGGA
    TCAGAACCAG AGATCATCAT CAATACAGCT CTGACGAGTC CTGAAGGTCT GGCTGTGGAT
    GTGGCCCGAA GGAGATTGTT TTGGGTGGAT AGCACACTGG ACAAGATCGA AACGGCCAAT
    CTCGATGGAA GTGACAGACA TGTTCTGTTC GCCACAGATT TAGTAAATCC CAGAGCGATG
    ATCGTGGACT CGTCTACAGG AACCCTCTAC TGGACTGACT GGAACCGAGA GGCTCCTAAA
    ATCGAGAGCT CTTCTGTGGA TGGGCAAAAC CGAAGGGTTC TGGTACAGGA AGGCATAGGA
    CTGCCGAATG CCTTGACCTA CGACTCCACC ATGCGTCAGG TCTGCTGGGC CGATGCTGGA
    ACCAAGCGTC TGGAGTGTAT TTCACCAAAC GGGACAGGCA GACGCGTGAT CCAGAGTAAC
    CTGAACTACC CTTTCAGCAT GGTGTACTTC GCCAATCACT ACTACTACAC TGACTGGAGG
    AGGGACGGAG TCATCTCTCT AGGTCGAGAT AACCAGTTAA CAGATGAGTA CTTACCTGAT
    CAGAGGTCTC ATTTGTATGG TATCACAGTC GCCCCTCCAC AATGTTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.