mbd3b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mbd3b
Entrez Gene ID	321217
Full Name	methyl-CpG binding domain protein 3b
Synonyms	cb357,cb99,mdb3b,sb:cb357,sb:cb99,wu:fc41c05,zgc:111851
General protein information	Preferred Names methyl-CpG binding domain protein 3b Names methyl-CpG binding domain protein 3b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005170433.3	XP_005170490.1	methyl-CpG binding domain protein 3b isoform X2
XM_005170432.3	XP_005170489.1	methyl-CpG binding domain protein 3b isoform X1
XM_005170433.4	XP_005170490.1	methyl-CpG binding domain protein 3b isoform X2
NM_212580.1	NP_997745.1	methyl-CpG binding domain protein 3b
XM_005170432.4	XP_005170489.1	methyl-CpG binding domain protein 3b isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish.
Brocal I, White RJ, Dooley CM, Carruthers SN, Clark R, Hall A, Busch-Nentwich EM, Stemple DL, Kettleborough RN
BMC genomics17259(2016 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Specific NuRD components are required for fin regeneration in zebrafish.
Pfefferli C, M?ller F, Ja?wi?ska A, Wicky C
BMC biology1230(2014 Apr)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mbd3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mbd3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34228	NM_212580.1 Latest version!	Danio rerio methyl-CpG binding domain protein 3b (mbd3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa26844	XM_005170432.4 Latest version!	Danio rerio methyl-CpG binding domain protein 3b (mbd3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa26845	XM_005170433.4 Latest version!	Danio rerio methyl-CpG binding domain protein 3b (mbd3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
ODa26845	XM_005170433.3	Danio rerio methyl-CpG binding domain protein 3b (mbd3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa26844	XM_005170432.3	Danio rerio methyl-CpG binding domain protein 3b (mbd3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mbd3b ( NM_212580.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997745.1 Danio rerio methyl-CpG binding domain protein 3b (mbd3b), mRNA.

Price & Availability↑

CloneID	ODa34228
Clone ID Related Accession (Same CDS sequence)	NM_212580.1
Accession Version	NM_212580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	methyl-CpG binding domain protein 3b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY238338.1. ##Evidence-Data-START## Transcript exon combination :: AY238338.1, BC093115.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAAAA ACGACCCCAC AGGAAAGAAG TTCCGGAGCA AGCCTCAATT GGCTCGTTAC 
CTTGGCAACT CCATGGATCT CAGCTCTTTT GATTTCCGTA CTGGGAAGAT GCTCATGAGC 
AAACTGAATA AGAACAGACA GAGACCCAGA TACGACAACA ACAGCCAGTC AAAGGGCAAA 
CCCGACTTAA ATACATCCCT CCCCGTACGA CAGACGGCCT CCATCTTCAA ACAACCCGTT 
ACAAAAGTTA CCAACCACCC CAGCAATAAA GTTAAAACAG ACCCACAAAA GGCTATTGAC 
CAGCCCCGAC AGCTCTTTTG GGAGAAGAAG TTGAGTGGCC TCAATGCCTT TGACATAGCA 
GAAGAGTTGG TCAAAACAAT GGATCTCCCC AAAGGCTTAC AAGGAGTGGG CCCTGGGTGC 
ACAGATAAGA CCCTGCTGTC AGCGATTGCC AGTGCGCTTC ACACCAGCGC AGCTCCCATC 
ACCGGTCAAC TATCCGCCGC TGTGGAGAAA AACCCAGGCG TTTGGCTCAA CACAGCTCAG 
CCTCTTTGTA AGGCCTTCAT TGTCACAGAT GAGGATATCA GAAAGCAAGA GGAGCTGGTA 
TACAGTGTTC GGAAACGGCT GGAGGAGGCT TTGATGGCTG ACATGTTGGC TCACGTGGAG 
GAAACGGCTA GTGAGGGAGA AGCACTCAAA CAGGAGGGGA ATTGCACTGA TGAGAAACAG 
GAAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997745.1
CDS	1..729
Translation	MEKNDPTGKKFRSKPQLARYLGNSMDLSSFDFRTGKMLMSKLNKNRQRPRYDNNSQSKGKPDLNTSLPVRQTASIFKQPVTKVTNHPSNKVKTDPQKAIDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDKTLLSAIASALHTSAAPITGQLSAAVEKNPGVWLNTAQPLCKAFIVTDEDIRKQEELVYSVRKRLEEALMADMLAHVEETASEGEALKQEGNCTDEKQEV

Target ORF information:

RefSeq Version	NM_212580.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methyl-CpG binding domain protein 3b (mbd3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212580.1

ORF Insert Sequence:

ATGGAGAAAA ACGACCCCAC AGGAAAGAAG TTCCGGAGCA AGCCTCAATT GGCTCGTTAC 
CTTGGCAACT CCATGGATCT CAGCTCTTTT GATTTCCGTA CTGGGAAGAT GCTCATGAGC 
AAACTGAATA AGAACAGACA GAGACCCAGA TACGACAACA ACAGCCAGTC AAAGGGCAAA 
CCCGACTTAA ATACATCCCT CCCCGTACGA CAGACGGCCT CCATCTTCAA ACAACCCGTT 
ACAAAAGTTA CCAACCACCC CAGCAATAAA GTTAAAACAG ACCCACAAAA GGCTATTGAC 
CAGCCCCGAC AGCTCTTTTG GGAGAAGAAG TTGAGTGGCC TCAATGCCTT TGACATAGCA 
GAAGAGTTGG TCAAAACAAT GGATCTCCCC AAAGGCTTAC AAGGAGTGGG CCCTGGGTGC 
ACAGATAAGA CCCTGCTGTC AGCGATTGCC AGTGCGCTTC ACACCAGCGC AGCTCCCATC 
ACCGGTCAAC TATCCGCCGC TGTGGAGAAA AACCCAGGCG TTTGGCTCAA CACAGCTCAG 
CCTCTTTGTA AGGCCTTCAT TGTCACAGAT GAGGATATCA GAAAGCAAGA GGAGCTGGTA 
TACAGTGTTC GGAAACGGCT GGAGGAGGCT TTGATGGCTG ACATGTTGGC TCACGTGGAG 
GAAACGGCTA GTGAGGGAGA AGCACTCAAA CAGGAGGGGA ATTGCACTGA TGAGAAACAG 
GAAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbd3b ( XM_005170432.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170489.1 Danio rerio methyl-CpG binding domain protein 3b (mbd3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26844
Clone ID Related Accession (Same CDS sequence)	XM_005170432.3 , XM_005170432.4
Accession Version	XM_005170432.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	methyl-CpG binding domain protein 3b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170432.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAA ACGACAGAGG TGAGGAAGAG GAGGAAGAAC AGAGGCGGGA AAGGGGGGAG 
GCAGGTCGTT TGTATAGTTT ATGCCCCACA GGAAAGAAGT TCCGGAGCAA GCCTCAATTG 
GCTCGTTCCC TTGGCAACTC CATGGATCTC AGCTCTTTTG ATTTCCGTAC TGGGAAGATG 
CTCATGAGCA AACTGAATAA GAACAGACAG AGACCCAGAT ACGACAACAA CAGCCAGTCA 
AAGGGTAAAC CCGACTTAAA TACATCCCTC CCAGTACGAC AGACGGCCTC CATCTTCAAA 
CAACCCGTTA CAAAAGTTAC CAACCATCCC AGCAATAAAG TTAAAACAGA CCCACAAAAG 
GCTATTGACC AGCCCCGACA GCTCTTTTGG GAGAAGAAGT TGAGTGGCCT CAATGCCTTT 
GACATAGCAG AAGAGTTGGT CAAAACAATG GATCTCCCCA AAGGCTTACA AGGAGTGGGC 
CCTGGGTGCA CAGATAAGAC CCTGCTGTCA GCGATTGCCA GTGCGCTTCA CACCAGCGCA 
GCTCCCATCA CCGGTCAACT ATCCGCCGCT GTGGAGAAAA ACCCAGGCGT TTGGCTCAAC 
ACAGCTCAGC CTCTTTGTAA GGCCTTCATT GTCACAGATG AGGATATCAG AAAGCAAGAG 
GAGCTGGTAT ACAGTGTTCG GAAACGGCTG GAGGAGGCTT TGATGGCTGA CATGTTGGCT 
CACGTGGAGG AAACGGCTAG TGAGGGAGAA GCACTCAAAC AGGAGGGGAA TTGCACTGAT 
GAGAAACAGG AAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170489.1
CDS	162..959
Translation	MEKNDRGEEEEEEQRRERGEAGRLYSLCPTGKKFRSKPQLARSLGNSMDLSSFDFRTGKMLMSKLNKNRQRPRYDNNSQSKGKPDLNTSLPVRQTASIFKQPVTKVTNHPSNKVKTDPQKAIDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDKTLLSAIASALHTSAAPITGQLSAAVEKNPGVWLNTAQPLCKAFIVTDEDIRKQEELVYSVRKRLEEALMADMLAHVEETASEGEALKQEGNCTDEKQEV

Target ORF information:

RefSeq Version	XM_005170432.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio methyl-CpG binding domain protein 3b (mbd3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170432.3

ORF Insert Sequence:

ATGGAGAAAA ACGACAGAGG TGAGGAAGAG GAGGAAGAAC AGAGGCGGGA AAGGGGGGAG 
GCAGGTCGTT TGTATAGTTT ATGCCCCACA GGAAAGAAGT TCCGGAGCAA GCCTCAATTG 
GCTCGTTCCC TTGGCAACTC CATGGATCTC AGCTCTTTTG ATTTCCGTAC TGGGAAGATG 
CTCATGAGCA AACTGAATAA GAACAGACAG AGACCCAGAT ACGACAACAA CAGCCAGTCA 
AAGGGTAAAC CCGACTTAAA TACATCCCTC CCAGTACGAC AGACGGCCTC CATCTTCAAA 
CAACCCGTTA CAAAAGTTAC CAACCATCCC AGCAATAAAG TTAAAACAGA CCCACAAAAG 
GCTATTGACC AGCCCCGACA GCTCTTTTGG GAGAAGAAGT TGAGTGGCCT CAATGCCTTT 
GACATAGCAG AAGAGTTGGT CAAAACAATG GATCTCCCCA AAGGCTTACA AGGAGTGGGC 
CCTGGGTGCA CAGATAAGAC CCTGCTGTCA GCGATTGCCA GTGCGCTTCA CACCAGCGCA 
GCTCCCATCA CCGGTCAACT ATCCGCCGCT GTGGAGAAAA ACCCAGGCGT TTGGCTCAAC 
ACAGCTCAGC CTCTTTGTAA GGCCTTCATT GTCACAGATG AGGATATCAG AAAGCAAGAG 
GAGCTGGTAT ACAGTGTTCG GAAACGGCTG GAGGAGGCTT TGATGGCTGA CATGTTGGCT 
CACGTGGAGG AAACGGCTAG TGAGGGAGAA GCACTCAAAC AGGAGGGGAA TTGCACTGAT 
GAGAAACAGG AAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbd3b ( XM_005170432.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170489.1 Danio rerio methyl-CpG binding domain protein 3b (mbd3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26844
Clone ID Related Accession (Same CDS sequence)	XM_005170432.3 , XM_005170432.4
Accession Version	XM_005170432.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	methyl-CpG binding domain protein 3b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170432.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAA ACGACAGAGG TGAGGAAGAG GAGGAAGAAC AGAGGCGGGA AAGGGGGGAG 
GCAGGTCGTT TGTATAGTTT ATGCCCCACA GGAAAGAAGT TCCGGAGCAA GCCTCAATTG 
GCTCGTTCCC TTGGCAACTC CATGGATCTC AGCTCTTTTG ATTTCCGTAC TGGGAAGATG 
CTCATGAGCA AACTGAATAA GAACAGACAG AGACCCAGAT ACGACAACAA CAGCCAGTCA 
AAGGGTAAAC CCGACTTAAA TACATCCCTC CCAGTACGAC AGACGGCCTC CATCTTCAAA 
CAACCCGTTA CAAAAGTTAC CAACCATCCC AGCAATAAAG TTAAAACAGA CCCACAAAAG 
GCTATTGACC AGCCCCGACA GCTCTTTTGG GAGAAGAAGT TGAGTGGCCT CAATGCCTTT 
GACATAGCAG AAGAGTTGGT CAAAACAATG GATCTCCCCA AAGGCTTACA AGGAGTGGGC 
CCTGGGTGCA CAGATAAGAC CCTGCTGTCA GCGATTGCCA GTGCGCTTCA CACCAGCGCA 
GCTCCCATCA CCGGTCAACT ATCCGCCGCT GTGGAGAAAA ACCCAGGCGT TTGGCTCAAC 
ACAGCTCAGC CTCTTTGTAA GGCCTTCATT GTCACAGATG AGGATATCAG AAAGCAAGAG 
GAGCTGGTAT ACAGTGTTCG GAAACGGCTG GAGGAGGCTT TGATGGCTGA CATGTTGGCT 
CACGTGGAGG AAACGGCTAG TGAGGGAGAA GCACTCAAAC AGGAGGGGAA TTGCACTGAT 
GAGAAACAGG AAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170489.1
CDS	182..979
Translation	MEKNDRGEEEEEEQRRERGEAGRLYSLCPTGKKFRSKPQLARSLGNSMDLSSFDFRTGKMLMSKLNKNRQRPRYDNNSQSKGKPDLNTSLPVRQTASIFKQPVTKVTNHPSNKVKTDPQKAIDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDKTLLSAIASALHTSAAPITGQLSAAVEKNPGVWLNTAQPLCKAFIVTDEDIRKQEELVYSVRKRLEEALMADMLAHVEETASEGEALKQEGNCTDEKQEV

Target ORF information:

RefSeq Version	XM_005170432.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methyl-CpG binding domain protein 3b (mbd3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170432.4

ORF Insert Sequence:

ATGGAGAAAA ACGACAGAGG TGAGGAAGAG GAGGAAGAAC AGAGGCGGGA AAGGGGGGAG 
GCAGGTCGTT TGTATAGTTT ATGCCCCACA GGAAAGAAGT TCCGGAGCAA GCCTCAATTG 
GCTCGTTCCC TTGGCAACTC CATGGATCTC AGCTCTTTTG ATTTCCGTAC TGGGAAGATG 
CTCATGAGCA AACTGAATAA GAACAGACAG AGACCCAGAT ACGACAACAA CAGCCAGTCA 
AAGGGTAAAC CCGACTTAAA TACATCCCTC CCAGTACGAC AGACGGCCTC CATCTTCAAA 
CAACCCGTTA CAAAAGTTAC CAACCATCCC AGCAATAAAG TTAAAACAGA CCCACAAAAG 
GCTATTGACC AGCCCCGACA GCTCTTTTGG GAGAAGAAGT TGAGTGGCCT CAATGCCTTT 
GACATAGCAG AAGAGTTGGT CAAAACAATG GATCTCCCCA AAGGCTTACA AGGAGTGGGC 
CCTGGGTGCA CAGATAAGAC CCTGCTGTCA GCGATTGCCA GTGCGCTTCA CACCAGCGCA 
GCTCCCATCA CCGGTCAACT ATCCGCCGCT GTGGAGAAAA ACCCAGGCGT TTGGCTCAAC 
ACAGCTCAGC CTCTTTGTAA GGCCTTCATT GTCACAGATG AGGATATCAG AAAGCAAGAG 
GAGCTGGTAT ACAGTGTTCG GAAACGGCTG GAGGAGGCTT TGATGGCTGA CATGTTGGCT 
CACGTGGAGG AAACGGCTAG TGAGGGAGAA GCACTCAAAC AGGAGGGGAA TTGCACTGAT 
GAGAAACAGG AAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbd3b ( XM_005170433.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170490.1 Danio rerio methyl-CpG binding domain protein 3b (mbd3b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa26845
Clone ID Related Accession (Same CDS sequence)	XM_005170433.3 , XM_005170433.4
Accession Version	XM_005170433.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	methyl-CpG binding domain protein 3b isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170433.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAA ACGAAGGTGA GGAAGAGGAG GAAGAACAGA GGCGGGAAAG GGGGGAGGCA 
GGTCGTTTGT ATAGTTTATG CCCCACAGGA AAGAAGTTCC GGAGCAAGCC TCAATTGGCT 
CGTTCCCTTG GCAACTCCAT GGATCTCAGC TCTTTTGATT TCCGTACTGG GAAGATGCTC 
ATGAGCAAAC TGAATAAGAA CAGACAGAGA CCCAGATACG ACAACAACAG CCAGTCAAAG 
GGTAAACCCG ACTTAAATAC ATCCCTCCCA GTACGACAGA CGGCCTCCAT CTTCAAACAA 
CCCGTTACAA AAGTTACCAA CCATCCCAGC AATAAAGTTA AAACAGACCC ACAAAAGGCT 
ATTGACCAGC CCCGACAGCT CTTTTGGGAG AAGAAGTTGA GTGGCCTCAA TGCCTTTGAC 
ATAGCAGAAG AGTTGGTCAA AACAATGGAT CTCCCCAAAG GCTTACAAGG AGTGGGCCCT 
GGGTGCACAG ATAAGACCCT GCTGTCAGCG ATTGCCAGTG CGCTTCACAC CAGCGCAGCT 
CCCATCACCG GTCAACTATC CGCCGCTGTG GAGAAAAACC CAGGCGTTTG GCTCAACACA 
GCTCAGCCTC TTTGTAAGGC CTTCATTGTC ACAGATGAGG ATATCAGAAA GCAAGAGGAG 
CTGGTATACA GTGTTCGGAA ACGGCTGGAG GAGGCTTTGA TGGCTGACAT GTTGGCTCAC 
GTGGAGGAAA CGGCTAGTGA GGGAGAAGCA CTCAAACAGG AGGGGAATTG CACTGATGAG 
AAACAGGAAG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170490.1
CDS	162..956
Translation	MEKNEGEEEEEEQRRERGEAGRLYSLCPTGKKFRSKPQLARSLGNSMDLSSFDFRTGKMLMSKLNKNRQRPRYDNNSQSKGKPDLNTSLPVRQTASIFKQPVTKVTNHPSNKVKTDPQKAIDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDKTLLSAIASALHTSAAPITGQLSAAVEKNPGVWLNTAQPLCKAFIVTDEDIRKQEELVYSVRKRLEEALMADMLAHVEETASEGEALKQEGNCTDEKQEV

Target ORF information:

RefSeq Version	XM_005170433.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio methyl-CpG binding domain protein 3b (mbd3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170433.3

ORF Insert Sequence:

ATGGAGAAAA ACGAAGGTGA GGAAGAGGAG GAAGAACAGA GGCGGGAAAG GGGGGAGGCA 
GGTCGTTTGT ATAGTTTATG CCCCACAGGA AAGAAGTTCC GGAGCAAGCC TCAATTGGCT 
CGTTCCCTTG GCAACTCCAT GGATCTCAGC TCTTTTGATT TCCGTACTGG GAAGATGCTC 
ATGAGCAAAC TGAATAAGAA CAGACAGAGA CCCAGATACG ACAACAACAG CCAGTCAAAG 
GGTAAACCCG ACTTAAATAC ATCCCTCCCA GTACGACAGA CGGCCTCCAT CTTCAAACAA 
CCCGTTACAA AAGTTACCAA CCATCCCAGC AATAAAGTTA AAACAGACCC ACAAAAGGCT 
ATTGACCAGC CCCGACAGCT CTTTTGGGAG AAGAAGTTGA GTGGCCTCAA TGCCTTTGAC 
ATAGCAGAAG AGTTGGTCAA AACAATGGAT CTCCCCAAAG GCTTACAAGG AGTGGGCCCT 
GGGTGCACAG ATAAGACCCT GCTGTCAGCG ATTGCCAGTG CGCTTCACAC CAGCGCAGCT 
CCCATCACCG GTCAACTATC CGCCGCTGTG GAGAAAAACC CAGGCGTTTG GCTCAACACA 
GCTCAGCCTC TTTGTAAGGC CTTCATTGTC ACAGATGAGG ATATCAGAAA GCAAGAGGAG 
CTGGTATACA GTGTTCGGAA ACGGCTGGAG GAGGCTTTGA TGGCTGACAT GTTGGCTCAC 
GTGGAGGAAA CGGCTAGTGA GGGAGAAGCA CTCAAACAGG AGGGGAATTG CACTGATGAG 
AAACAGGAAG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbd3b ( XM_005170433.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170490.1 Danio rerio methyl-CpG binding domain protein 3b (mbd3b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa26845
Clone ID Related Accession (Same CDS sequence)	XM_005170433.3 , XM_005170433.4
Accession Version	XM_005170433.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	methyl-CpG binding domain protein 3b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170433.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAA ACGAAGGTGA GGAAGAGGAG GAAGAACAGA GGCGGGAAAG GGGGGAGGCA 
GGTCGTTTGT ATAGTTTATG CCCCACAGGA AAGAAGTTCC GGAGCAAGCC TCAATTGGCT 
CGTTCCCTTG GCAACTCCAT GGATCTCAGC TCTTTTGATT TCCGTACTGG GAAGATGCTC 
ATGAGCAAAC TGAATAAGAA CAGACAGAGA CCCAGATACG ACAACAACAG CCAGTCAAAG 
GGTAAACCCG ACTTAAATAC ATCCCTCCCA GTACGACAGA CGGCCTCCAT CTTCAAACAA 
CCCGTTACAA AAGTTACCAA CCATCCCAGC AATAAAGTTA AAACAGACCC ACAAAAGGCT 
ATTGACCAGC CCCGACAGCT CTTTTGGGAG AAGAAGTTGA GTGGCCTCAA TGCCTTTGAC 
ATAGCAGAAG AGTTGGTCAA AACAATGGAT CTCCCCAAAG GCTTACAAGG AGTGGGCCCT 
GGGTGCACAG ATAAGACCCT GCTGTCAGCG ATTGCCAGTG CGCTTCACAC CAGCGCAGCT 
CCCATCACCG GTCAACTATC CGCCGCTGTG GAGAAAAACC CAGGCGTTTG GCTCAACACA 
GCTCAGCCTC TTTGTAAGGC CTTCATTGTC ACAGATGAGG ATATCAGAAA GCAAGAGGAG 
CTGGTATACA GTGTTCGGAA ACGGCTGGAG GAGGCTTTGA TGGCTGACAT GTTGGCTCAC 
GTGGAGGAAA CGGCTAGTGA GGGAGAAGCA CTCAAACAGG AGGGGAATTG CACTGATGAG 
AAACAGGAAG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170490.1
CDS	182..976
Translation	MEKNEGEEEEEEQRRERGEAGRLYSLCPTGKKFRSKPQLARSLGNSMDLSSFDFRTGKMLMSKLNKNRQRPRYDNNSQSKGKPDLNTSLPVRQTASIFKQPVTKVTNHPSNKVKTDPQKAIDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDKTLLSAIASALHTSAAPITGQLSAAVEKNPGVWLNTAQPLCKAFIVTDEDIRKQEELVYSVRKRLEEALMADMLAHVEETASEGEALKQEGNCTDEKQEV

Target ORF information:

RefSeq Version	XM_005170433.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methyl-CpG binding domain protein 3b (mbd3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170433.4

ORF Insert Sequence:

ATGGAGAAAA ACGAAGGTGA GGAAGAGGAG GAAGAACAGA GGCGGGAAAG GGGGGAGGCA 
GGTCGTTTGT ATAGTTTATG CCCCACAGGA AAGAAGTTCC GGAGCAAGCC TCAATTGGCT 
CGTTCCCTTG GCAACTCCAT GGATCTCAGC TCTTTTGATT TCCGTACTGG GAAGATGCTC 
ATGAGCAAAC TGAATAAGAA CAGACAGAGA CCCAGATACG ACAACAACAG CCAGTCAAAG 
GGTAAACCCG ACTTAAATAC ATCCCTCCCA GTACGACAGA CGGCCTCCAT CTTCAAACAA 
CCCGTTACAA AAGTTACCAA CCATCCCAGC AATAAAGTTA AAACAGACCC ACAAAAGGCT 
ATTGACCAGC CCCGACAGCT CTTTTGGGAG AAGAAGTTGA GTGGCCTCAA TGCCTTTGAC 
ATAGCAGAAG AGTTGGTCAA AACAATGGAT CTCCCCAAAG GCTTACAAGG AGTGGGCCCT 
GGGTGCACAG ATAAGACCCT GCTGTCAGCG ATTGCCAGTG CGCTTCACAC CAGCGCAGCT 
CCCATCACCG GTCAACTATC CGCCGCTGTG GAGAAAAACC CAGGCGTTTG GCTCAACACA 
GCTCAGCCTC TTTGTAAGGC CTTCATTGTC ACAGATGAGG ATATCAGAAA GCAAGAGGAG 
CTGGTATACA GTGTTCGGAA ACGGCTGGAG GAGGCTTTGA TGGCTGACAT GTTGGCTCAC 
GTGGAGGAAA CGGCTAGTGA GGGAGAAGCA CTCAAACAGG AGGGGAATTG CACTGATGAG 
AAACAGGAAG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish.
BMC genomics17259(2016 Mar)
Brocal I,White RJ,Dooley CM,Carruthers SN,Clark R,Hall A,Busch-Nentwich EM,Stemple DL,Kettleborough RN

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Specific NuRD components are required for fin regeneration in zebrafish.
BMC biology1230(2014 Apr)
Pfefferli C,M?ller F,Ja?wi?ska A,Wicky C

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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mbd3b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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