robo3 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005172637.3	XP_005172694.1	roundabout homolog 3 isoform X2
XM_005172638.3	XP_005172695.1	roundabout homolog 3 isoform X3
XM_005172638.4	XP_005172695.1	roundabout homolog 3 isoform X3
NM_001328416.1	NP_001315345.1	roundabout homolog 3 isoform 1
NM_131482.3	NP_571557.2	roundabout homolog 3 isoform 2 precursor
XM_017357931.1	XP_017213420.1	roundabout homolog 3 isoform X4
XM_005172636.4	XP_005172693.1	roundabout homolog 3 isoform X1
XM_005172636.3	XP_005172693.1	roundabout homolog 3 isoform X1
XM_005172637.4	XP_005172694.1	roundabout homolog 3 isoform X2
XM_017357931.2	XP_017213420.1	roundabout homolog 3 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa98472	NM_131482.3 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa54003	XM_017357931.2 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa16202	NM_001328416.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa16199	XM_005172636.4 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa16200	XM_005172637.4 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa16201	XM_005172638.4 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa16201	XM_005172638.3	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa16199	XM_005172636.3	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa16200	XM_005172637.3	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa54003	XM_017357931.1	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

robo3 ( NM_131482.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571557.2 Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa98472
Clone ID Related Accession (Same CDS sequence)	NM_131482.3
Accession Version	NM_131482.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-30
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 3 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845320.10. On Jun 16, 2016 this sequence version replaced NM_131482.2. Transcript Variant: This variant (2) differs in the 5' UTR, uses an alternate translation start site, and uses an alternate splice site in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CU638801.1, AF337036.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTTC GCAGGACTTT GGATGCGCTG GTCGTTGTTT TTGCCCTCAT ATGCTTGTCA 
CAAGGATCCC GGCCTAGGTT GGAAGATGCT GCACCCCGGA TTGTTGAACA CCCCTCAGAC 
CTCATTGTTT CCAAAGGTGA GCCTGCAACT TTGAACTGTA AGGCCGAAGG ACGACCGACC 
CCCATGGTGG AGTGGTACAA AGATGGCGAG CGAGTGGAAA CCGACCGGGA GGATCCACGC 
TCCCACCGCA TGCTCCTCCC CAGCGGCTCC CTCTTCTTTC TCCGAATTGT TCACGGAAGG 
CGCAGCAAGC CAGATGAAGG CGTGTACGTG TGCGTAGCAA GGAATTACTT GGGCGAGGCC 
GTCAGTCGTA ATGCCTCTTT GGAGGTCGCA ATTCTCCGGG ATGATTTCCG ACAGACCCCC 
AGTGATGTGG TGGTGGCGGC AGGAGAGCCG GCAGTGATGG AGTGCATCCC TCCACGAGGA 
CACCCAGAAC CCACCATCTC CTGGAAGAGG AACAATGTGA AAGTCAACGA CAAGGACGAG 
AGGATAACGA TCCGAGGAGG GAAGCTGATG ATTTCTAACA CGCGCAAAAG TGATGCCGGG 
ATGTATGTCT GTGTCGGCAC CAATATGGTT GGAGAGAAGG ACAGCGATCC TGCTGAGCTT 
GTAGTGTTTG AGCGGCCCAT GTTTGTGCGC AGACCTGTGA ATCAGGTGGT TCTTGCTGAT 
GATACAGTGG ATTTCCAGTG TGAGGTTCAA GGAGACCCAG CTCCTACCAT ACGCTGGAAG 
AAAGAGGAAG GAGAACTGCC TCGGGGAAGG TTTGAGATCC GTGCCGACAA CAGCCTGCGT 
CTGACTCAGG TGAAGGCTGA AGATGAAGGC TCCTACACCT GTTTATCAGA GAACAGCGTG 
GGCAAAGCTG AAGCATCCGG ATCGCTTCAG GTCCACGTTC CTCCTCAGAT TGTAGTGCGT 
CCGCGGGATC AGATCTCTGC CCAGGGCCGC ACTGTCACCT TTCTCTGCGG AACGAAGGGA 
AATCCCCCTC CTGCTGTTTT CTGGCAGAAA GAAGGCAGTC AGGTCCTGCT GTTTCCCAGT 
CAGCCGCCCT CACAGTCAGG CCGTTTTTCC GTGTCTCTGA GCGGAGAGCT TACCATCACA 
GATGTGCACA GCGAGGATTC TGGCTACTAC ATCTGCCAGG CTATCAGTGT GGCGGGCAGC 
ATCTTGACCA AAGCACTGCT GGAGGTGGAA AGCACTCCAT CTGACCGGAT CCCTCCCATC 
ATCCGCCAAG GCCCGGCCAA CCAGACGCTA GCTCCGGGCA CCACGGCCCA ATTGCAGTGC 
CACGTGATGG GCAACCCTCT TCCGAGCATC CAATGGGAGC GCGACGGACA ACGAATCCTG 
GGCATCGACG AGCGCATTAG CCTAATGGAA AACGGCACTT TGCAAATCAC AGCCCTGCAG 
GAGACGGACT CCGGGGCCTA CACCTGTGTT GCTTCCAGTC TGAGTGGAGA GACGAGCTGG 
AGTGGTGTGC TCACAGTAAA AGAGAGTGGT GGTCTATCAG CATCTCCGGT ATCAGAGCCC 
TATCAACTGC CCGGTCCTCC TCAGAAACCT GTCGTAACGG ATGTAACCCG TAACAGTGTC 
ACCCTCACCT GGCAACCCAA CGCACACGAA GGCGGCGCCG CCGTCACCTC TTACATCATC 
GAGGCCTTCA GTCAATCGGC AGGCAGTACG TGGCAGACGG TTGCAGATTT TGTGAAGCTG 
GAAAAGCACA CGGTCACCGG ATTGTCTCCG AACACCATTT ACCTGTTTAT TGTGCGAGCC 
GTCAATGCAT ATGGCCTGAG TGACCCAAGC CCAATTTCAG AGCCCGTACG CACACAAGAT 
GTGAGTCCCA CAGGTCAGGG TGTTGACCAT CGTCAGGTGC AGAGGGAGCT GGGAGAAATG 
TCTGTACAGC TTCACGAACC CGTGCTGTTG ACGGCCTCCA GTGTGAAGAT CAGCTGGAAC 
GTGGACCGTC AGTCGCAGTA CATCCAGGGT TATCGGCTGC TTTATCGGCC TGTCGGCGGT 
TCCTGGTCCC AGCAGGAAGT GAAGGGTGCA ACAGAGCGTA GCGCCGTCAT CGCTAACCTG 
CTCAAAGGCA CCGAGTACGA GATTAAAATA CGACCCTACT TTAACGAGTT TCAGGGCCTG 
GACAGCCGAT CGCAGACACT CCGCACGCCA GAGGAGGTGC CGAGTGCTCC TCCTCGAGCT 
GTGAATGTGG CCACAGTGAA GCTCAGTAAC AGCTCTAGCA TCAGTGTTTC ATGGCAGCCT 
CCACCCACAG ACATGCAGAA TGGAGTCATT CAGGAGTACA AGGTTTGGTG TCTGGGCAAT 
GATTCTCAGA CACGCTACAA CATCAATCAG ACGGTGGACG GCAGTGTGTT AAGCACTGTA 
CTGAAGGGTC TGCTGCCTGG CGTCCTGTAC CAGGTGGAGG TGGCAGCAGT GACCAGTGCA 
GGAGTGGGCA AACACAGCCA ACCCGTATCT GTTCTTATCA AATTGCCGGC AGAGCAGCCC 
ACAGTCACAG GTGGAGGCAG TGAATCTGAG GAGAATGTAA GTCTGGCGGA GCAGATCACA 
GACGTGGTGA AGCAGCCTGC CTTCATCGCA GGCATTGGCG GAGCATGCTG GGTAATCCTG 
ATGGGATTCA GCGTGTGGAT CTACTGCCGA CGCAAGAAGA GAAAAGAACT CAGCCATTAC 
ACTGCGTCAT TTGCATACAC ACCGGCAGTT GGTTTTCCAC ATGGTGAAAG TTCTGGACTT 
AACGGAAGGC CTGGTATGCT GGGTGCTAAC ATGGGAAATT ATCCATGGCT GGCTGATTCT 
TGGCCTACTA CCAATCTTGT GCACAACGGG AAAGACTCAG TGAACTGCTG TACATCTAAA 
CACGAAACAC CGGAGAGATA CTATAACGAG GCGGGAATCT CTAATTACCT CAATCAAAAT 
GAGAAGTACA GCGCAGACTC CAACGAGGGG CCGATCTACA GCACCATCGA CCCCACCAGC 
GAAGACTTAC GCAGTTTTAC CGCTCAGTAT TCTCAGCACA CCACACCCTA CGCCAGCACT 
CCCATTATGC CTTTCACCAA CCAGGCGCCC TGCAGCCCTG AGCAAGATGG CAGCCACTGG 
ATGACGCAGC CGGGAACCTC CAATGCTCAG TATGCCCAGC CAGACCGATC CAAGACTGAG 
AACGTAAAAC AACCAAAGCA GAAGTTGATG GGGAAGCCGG TGAAAACCCC ATCTCTCAAC 
TGGATGGATG CGCTGCCGCC TCCACCCATC TCTGGAGATC TGGAGCAGTG TGACGAGGAG 
GATGATAACG AACTAATACA AGACATGGGG TCTGATGAGG AAGAGTGGTG TCCTCCTCTT 
CCCGAGCGGT CTTATCTCAT GGACGGCATG GTGGAGGACG GTCCAGTTCC ACCACCAAGA 
GGAGAAGCTT CTTCACCCAC TACGTCCTAC AGCCACCAGT CCACCGCCAC CTTAACCCCT 
TCTCCCCGAG ACGATGGACA CTTCCACGAT ATACCACGCT TACACCAGCT CGAACAACCA 
CACATGACAC GCCGGTTGCC GTGTGGACCA GTGCCTGTGC CACGAGCACC AAGCCCACCT 
CTAACCCAAT CCAACCCAAA CCTGTCAGCC AATCAGGAGT GTCCTGACAA CACTGTGCCA 
CACCGTTCAG CTCATAAACG AGACCTGAGC CAAGGAGCTG CACAAAGTGT AGAGAACATC 
AGCAACACCA CACCAGGTCG AGCGAGGCCC TCCGCAGACC ACACATCGTC TGGGCAGAAA 
TCCAAGGTGA AGAAGAAAGC TTCCAAGAGC GCTCAATACC GGAGAGATAT ACTGCAGGGC 
GATCTGCCTC CTCCACCAGA GCCTCCACCC GAAGACCAGG ATAATTGCGC TCATGTAGGG 
GGCTTCGAAT GCGGGCAGAC GTCCCTCGAG AGAGCCGGCA GCTCCAATTC GTCCATGGAG 
AGGAGAGAGC AGAGCACACC CAACCGGAAA AACTCGGCCC ATAGACGGAT AGACGCAGAT 
GACGATTTAA TTCCTTACAA CAAGCCTAAC TTCCTGTCCC GAGGTCAGAT GTCCAGCAAT 
TGCTCCACAA CAGGAAGTTC ATCATCACGA GGGTCAACGG GGTCGCGAGG GCAAGGAACG 
GGCAGGAAGA GAAATGATGA GATGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571557.2
CDS	435..4604
Translation	MEFRRTLDALVVVFALICLSQGSRPRLEDAAPRIVEHPSDLIVSKGEPATLNCKAEGRPTPMVEWYKDGERVETDREDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYVCVARNYLGEAVSRNASLEVAILRDDFRQTPSDVVVAAGEPAVMECIPPRGHPEPTISWKRNNVKVNDKDERITIRGGKLMISNTRKSDAGMYVCVGTNMVGEKDSDPAELVVFERPMFVRRPVNQVVLADDTVDFQCEVQGDPAPTIRWKKEEGELPRGRFEIRADNSLRLTQVKAEDEGSYTCLSENSVGKAEASGSLQVHVPPQIVVRPRDQISAQGRTVTFLCGTKGNPPPAVFWQKEGSQVLLFPSQPPSQSGRFSVSLSGELTITDVHSEDSGYYICQAISVAGSILTKALLEVESTPSDRIPPIIRQGPANQTLAPGTTAQLQCHVMGNPLPSIQWERDGQRILGIDERISLMENGTLQITALQETDSGAYTCVASSLSGETSWSGVLTVKESGGLSASPVSEPYQLPGPPQKPVVTDVTRNSVTLTWQPNAHEGGAAVTSYIIEAFSQSAGSTWQTVADFVKLEKHTVTGLSPNTIYLFIVRAVNAYGLSDPSPISEPVRTQDVSPTGQGVDHRQVQRELGEMSVQLHEPVLLTASSVKISWNVDRQSQYIQGYRLLYRPVGGSWSQQEVKGATERSAVIANLLKGTEYEIKIRPYFNEFQGLDSRSQTLRTPEEVPSAPPRAVNVATVKLSNSSSISVSWQPPPTDMQNGVIQEYKVWCLGNDSQTRYNINQTVDGSVLSTVLKGLLPGVLYQVEVAAVTSAGVGKHSQPVSVLIKLPAEQPTVTGGGSESEENVSLAEQITDVVKQPAFIAGIGGACWVILMGFSVWIYCRRKKRKELSHYTASFAYTPAVGFPHGESSGLNGRPGMLGANMGNYPWLADSWPTTNLVHNGKDSVNCCTSKHETPERYYNEAGISNYLNQNEKYSADSNEGPIYSTIDPTSEDLRSFTAQYSQHTTPYASTPIMPFTNQAPCSPEQDGSHWMTQPGTSNAQYAQPDRSKTENVKQPKQKLMGKPVKTPSLNWMDALPPPPISGDLEQCDEEDDNELIQDMGSDEEEWCPPLPERSYLMDGMVEDGPVPPPRGEASSPTTSYSHQSTATLTPSPRDDGHFHDIPRLHQLEQPHMTRRLPCGPVPVPRAPSPPLTQSNPNLSANQECPDNTVPHRSAHKRDLSQGAAQSVENISNTTPGRARPSADHTSSGQKSKVKKKASKSAQYRRDILQGDLPPPPEPPPEDQDNCAHVGGFECGQTSLERAGSSNSSMERREQSTPNRKNSAHRRIDADDDLIPYNKPNFLSRGQMSSNCSTTGSSSSRGSTGSRGQGTGRKRNDEMR

Target ORF information:

RefSeq Version	NM_131482.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131482.3

ORF Insert Sequence:

ATGGAGTTTC GCAGGACTTT GGATGCGCTG GTCGTTGTTT TTGCCCTCAT ATGCTTGTCA 
CAAGGATCCC GGCCTAGGTT GGAAGATGCT GCACCCCGGA TTGTTGAACA CCCCTCAGAC 
CTCATTGTTT CCAAAGGTGA GCCTGCAACT TTGAACTGTA AGGCCGAAGG ACGACCGACC 
CCCATGGTGG AGTGGTACAA AGATGGCGAG CGAGTGGAAA CCGACCGGGA GGATCCACGC 
TCCCACCGCA TGCTCCTCCC CAGCGGCTCC CTCTTCTTTC TCCGAATTGT TCACGGAAGG 
CGCAGCAAGC CAGATGAAGG CGTGTACGTG TGCGTAGCAA GGAATTACTT GGGCGAGGCC 
GTCAGTCGTA ATGCCTCTTT GGAGGTCGCA ATTCTCCGGG ATGATTTCCG ACAGACCCCC 
AGTGATGTGG TGGTGGCGGC AGGAGAGCCG GCAGTGATGG AGTGCATCCC TCCACGAGGA 
CACCCAGAAC CCACCATCTC CTGGAAGAGG AACAATGTGA AAGTCAACGA CAAGGACGAG 
AGGATAACGA TCCGAGGAGG GAAGCTGATG ATTTCTAACA CGCGCAAAAG TGATGCCGGG 
ATGTATGTCT GTGTCGGCAC CAATATGGTT GGAGAGAAGG ACAGCGATCC TGCTGAGCTT 
GTAGTGTTTG AGCGGCCCAT GTTTGTGCGC AGACCTGTGA ATCAGGTGGT TCTTGCTGAT 
GATACAGTGG ATTTCCAGTG TGAGGTTCAA GGAGACCCAG CTCCTACCAT ACGCTGGAAG 
AAAGAGGAAG GAGAACTGCC TCGGGGAAGG TTTGAGATCC GTGCCGACAA CAGCCTGCGT 
CTGACTCAGG TGAAGGCTGA AGATGAAGGC TCCTACACCT GTTTATCAGA GAACAGCGTG 
GGCAAAGCTG AAGCATCCGG ATCGCTTCAG GTCCACGTTC CTCCTCAGAT TGTAGTGCGT 
CCGCGGGATC AGATCTCTGC CCAGGGCCGC ACTGTCACCT TTCTCTGCGG AACGAAGGGA 
AATCCCCCTC CTGCTGTTTT CTGGCAGAAA GAAGGCAGTC AGGTCCTGCT GTTTCCCAGT 
CAGCCGCCCT CACAGTCAGG CCGTTTTTCC GTGTCTCTGA GCGGAGAGCT TACCATCACA 
GATGTGCACA GCGAGGATTC TGGCTACTAC ATCTGCCAGG CTATCAGTGT GGCGGGCAGC 
ATCTTGACCA AAGCACTGCT GGAGGTGGAA AGCACTCCAT CTGACCGGAT CCCTCCCATC 
ATCCGCCAAG GCCCGGCCAA CCAGACGCTA GCTCCGGGCA CCACGGCCCA ATTGCAGTGC 
CACGTGATGG GCAACCCTCT TCCGAGCATC CAATGGGAGC GCGACGGACA ACGAATCCTG 
GGCATCGACG AGCGCATTAG CCTAATGGAA AACGGCACTT TGCAAATCAC AGCCCTGCAG 
GAGACGGACT CCGGGGCCTA CACCTGTGTT GCTTCCAGTC TGAGTGGAGA GACGAGCTGG 
AGTGGTGTGC TCACAGTAAA AGAGAGTGGT GGTCTATCAG CATCTCCGGT ATCAGAGCCC 
TATCAACTGC CCGGTCCTCC TCAGAAACCT GTCGTAACGG ATGTAACCCG TAACAGTGTC 
ACCCTCACCT GGCAACCCAA CGCACACGAA GGCGGCGCCG CCGTCACCTC TTACATCATC 
GAGGCCTTCA GTCAATCGGC AGGCAGTACG TGGCAGACGG TTGCAGATTT TGTGAAGCTG 
GAAAAGCACA CGGTCACCGG ATTGTCTCCG AACACCATTT ACCTGTTTAT TGTGCGAGCC 
GTCAATGCAT ATGGCCTGAG TGACCCAAGC CCAATTTCAG AGCCCGTACG CACACAAGAT 
GTGAGTCCCA CAGGTCAGGG TGTTGACCAT CGTCAGGTGC AGAGGGAGCT GGGAGAAATG 
TCTGTACAGC TTCACGAACC CGTGCTGTTG ACGGCCTCCA GTGTGAAGAT CAGCTGGAAC 
GTGGACCGTC AGTCGCAGTA CATCCAGGGT TATCGGCTGC TTTATCGGCC TGTCGGCGGT 
TCCTGGTCCC AGCAGGAAGT GAAGGGTGCA ACAGAGCGTA GCGCCGTCAT CGCTAACCTG 
CTCAAAGGCA CCGAGTACGA GATTAAAATA CGACCCTACT TTAACGAGTT TCAGGGCCTG 
GACAGCCGAT CGCAGACACT CCGCACGCCA GAGGAGGTGC CGAGTGCTCC TCCTCGAGCT 
GTGAATGTGG CCACAGTGAA GCTCAGTAAC AGCTCTAGCA TCAGTGTTTC ATGGCAGCCT 
CCACCCACAG ACATGCAGAA TGGAGTCATT CAGGAGTACA AGGTTTGGTG TCTGGGCAAT 
GATTCTCAGA CACGCTACAA CATCAATCAG ACGGTGGACG GCAGTGTGTT AAGCACTGTA 
CTGAAGGGTC TGCTGCCTGG CGTCCTGTAC CAGGTGGAGG TGGCAGCAGT GACCAGTGCA 
GGAGTGGGCA AACACAGCCA ACCCGTATCT GTTCTTATCA AATTGCCGGC AGAGCAGCCC 
ACAGTCACAG GTGGAGGCAG TGAATCTGAG GAGAATGTAA GTCTGGCGGA GCAGATCACA 
GACGTGGTGA AGCAGCCTGC CTTCATCGCA GGCATTGGCG GAGCATGCTG GGTAATCCTG 
ATGGGATTCA GCGTGTGGAT CTACTGCCGA CGCAAGAAGA GAAAAGAACT CAGCCATTAC 
ACTGCGTCAT TTGCATACAC ACCGGCAGTT GGTTTTCCAC ATGGTGAAAG TTCTGGACTT 
AACGGAAGGC CTGGTATGCT GGGTGCTAAC ATGGGAAATT ATCCATGGCT GGCTGATTCT 
TGGCCTACTA CCAATCTTGT GCACAACGGG AAAGACTCAG TGAACTGCTG TACATCTAAA 
CACGAAACAC CGGAGAGATA CTATAACGAG GCGGGAATCT CTAATTACCT CAATCAAAAT 
GAGAAGTACA GCGCAGACTC CAACGAGGGG CCGATCTACA GCACCATCGA CCCCACCAGC 
GAAGACTTAC GCAGTTTTAC CGCTCAGTAT TCTCAGCACA CCACACCCTA CGCCAGCACT 
CCCATTATGC CTTTCACCAA CCAGGCGCCC TGCAGCCCTG AGCAAGATGG CAGCCACTGG 
ATGACGCAGC CGGGAACCTC CAATGCTCAG TATGCCCAGC CAGACCGATC CAAGACTGAG 
AACGTAAAAC AACCAAAGCA GAAGTTGATG GGGAAGCCGG TGAAAACCCC ATCTCTCAAC 
TGGATGGATG CGCTGCCGCC TCCACCCATC TCTGGAGATC TGGAGCAGTG TGACGAGGAG 
GATGATAACG AACTAATACA AGACATGGGG TCTGATGAGG AAGAGTGGTG TCCTCCTCTT 
CCCGAGCGGT CTTATCTCAT GGACGGCATG GTGGAGGACG GTCCAGTTCC ACCACCAAGA 
GGAGAAGCTT CTTCACCCAC TACGTCCTAC AGCCACCAGT CCACCGCCAC CTTAACCCCT 
TCTCCCCGAG ACGATGGACA CTTCCACGAT ATACCACGCT TACACCAGCT CGAACAACCA 
CACATGACAC GCCGGTTGCC GTGTGGACCA GTGCCTGTGC CACGAGCACC AAGCCCACCT 
CTAACCCAAT CCAACCCAAA CCTGTCAGCC AATCAGGAGT GTCCTGACAA CACTGTGCCA 
CACCGTTCAG CTCATAAACG AGACCTGAGC CAAGGAGCTG CACAAAGTGT AGAGAACATC 
AGCAACACCA CACCAGGTCG AGCGAGGCCC TCCGCAGACC ACACATCGTC TGGGCAGAAA 
TCCAAGGTGA AGAAGAAAGC TTCCAAGAGC GCTCAATACC GGAGAGATAT ACTGCAGGGC 
GATCTGCCTC CTCCACCAGA GCCTCCACCC GAAGACCAGG ATAATTGCGC TCATGTAGGG 
GGCTTCGAAT GCGGGCAGAC GTCCCTCGAG AGAGCCGGCA GCTCCAATTC GTCCATGGAG 
AGGAGAGAGC AGAGCACACC CAACCGGAAA AACTCGGCCC ATAGACGGAT AGACGCAGAT 
GACGATTTAA TTCCTTACAA CAAGCCTAAC TTCCTGTCCC GAGGTCAGAT GTCCAGCAAT 
TGCTCCACAA CAGGAAGTTC ATCATCACGA GGGTCAACGG GGTCGCGAGG GCAAGGAACG 
GGCAGGAAGA GAAATGATGA GATGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_017357931.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213420.1 Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa54003
Clone ID Related Accession (Same CDS sequence)	XM_017357931.1 , XM_017357931.2
Accession Version	XM_017357931.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 3 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTTC GCAGGACTTT GGATGCGCTG GTCGTTGTTT TTGCCCTCAT ATGCTTGTCA 
CAAGGATCCC GGCCTAGGTT GGAAGATGCT GCACCCCGGA TTGTTGAACA CCCCTCAGAC 
CTCATTGTTT CCAAAGGTGA GCCTGCAACT TTGAACTGTA AGGCCGAAGG ACGACCGACC 
CCCATGGTGG AGTGGTACAA AGATGGCGAG CGAGTGGAAA CCGACCGGGA GGATCCACGC 
TCCCACCGCA TGCTCCTCCC CAGCGGCTCC CTCTTCTTTC TCCGAATTGT TCACGGAAGG 
CGCAGCAAGC CAGATGAAGG CGTGTACGTG TGCGTAGCAA GGAATTACTT GGGCGAGGCC 
GTCAGTCGTA ATGCCTCTTT GGAGGTCGCA ATTCTCCGGG ATGATTTCCG ACAGACCCCC 
AGTGATGTGG TGGTGGCGGC AGGAGAGCCG GCAGTGATGG AGTGCATCCC TCCACGAGGA 
CACCCAGAAC CCACCATCTC CTGGAAGAGG AACAATGTGA AAGTCAACGA CAAGGACGAG 
AGGATAACGA TCCGAGGAGG GAAGCTGATG ATTTCTAACA CGCGCAAAAG TGATGCCGGG 
ATGTATGTCT GTGTCGGCAC CAATATGGTT GGAGAGAAGG ACAGCGATCC TGCTGAGCTT 
GTAGTGTTTG AGCGGCCCAT GTTTGTGCGC AGACCTGTGA ATCAGGTGGT TCTTGCTGAT 
GATACAGTGG ATTTCCAGTG TGAGGTTCAA GGAGACCCAG CTCCTACCAT ACGCTGGAAG 
AAAGAGGAAG GAGAACTGCC TCGGGGAAGG TTTGAGATCC GTGCCGACAA CAGCCTGCGT 
CTGACTCAGG TGAAGGCTGA AGATGAAGGC TCCTACACCT GTTTATCAGA GAACAGCGTG 
GGCAAAGCTG AAGCATCCGG ATCGCTTCAG GTCCACGTGG GCCCATCTCT TCCTCCTCAG 
ATTGTAGTGC GTCCGCGGGA TCAGATCTCT GCCCAGGGCC GCACTGTCAC CTTTCTCTGC 
GGAACGAAGG GAAATCCCCC TCCTGCTGTT TTCTGGCAGA AAGAAGGCAG TCAGGTCCTG 
CTGTTTCCCA GTCAGCCGCC CTCACAGTCA GGCCGTTTTT CCGTGTCTCT GAGCGGAGAG 
CTTACCATCA CAGATGTGCA CAGCGAGGAT TCTGGCTACT ACATCTGCCA GGCTATCAGT 
GTGGCGGGCA GCATCTTGAC CAAAGCACTG CTGGAGGTGG AAAGCACTCC ATCTGACCGG 
ATCCCTCCCA TCATCCGCCA AGGCCCGGCC AACCAGACGC TAGCTCCGGG CACCACGGCC 
CAATTGCAGT GCCACGTGAT GGGCAACCCT CTTCCGAGCA TCCAATGGGA GCGCGACGGA 
CAACGAATCC TGGGCATCGA CGAGCGCATT AGCCTAATGG AAAACGGCAC TTTGCAAATC 
ACAGCCCTGC AGGAGACGGA CTCCGGGGCC TACACCTGTG TTGCTTCCAG TCTGAGTGGA 
GAGACGAGCT GGAGTGGTGT GCTCACAGTA AAAGAGAGTG GTGGTCTATC AGCATCTCCG 
GTATCAGAGC CCTATCAACT GCCCGGTCCT CCTCAGAAAC CTGTCGTAAC GGATGTAACC 
CGTAACAGTG TCACCCTCAC CTGGCAACCC AACGCACACG AAGGCGGCGC CGCCGTCACC 
TCTTACATCA TCGAGGCCTT CAGTCAATCG GCAGGCAGTA CGTGGCAGAC GGTTGCAGAT 
TTTGTGAAGC TGGAAAAGCA CACGGTCACC GGATTGTCTC CGAACACCAT TTACCTGTTT 
ATTGTGCGAG CCGTCAATGC ATATGGCCTG AGTGACCCAA GCCCAATTTC AGAGCCCGTA 
CGCACACAAG ATGTGAGTCC CACAGGTCAG GGTGTTGACC ATCGTCAGGT GCAGAGGGAG 
CTGGGAGAAA TGTCTGTACA GCTTCACGAA CCCGTGCTGT TGACGGCCTC CAGTGTGAAG 
ATCAGCTGGA ACGTGGACCG TCAGTCGCAG TACATCCAGG GTTATCGGCT GCTTTATCGG 
CCTGTCGGCG GTTCCTGGTC CCAGCAGGAA GTGAAGGGTG CAACAGAGCG TAGCGCCGTC 
ATCGCTAACC TGCTCAAAGG CACCGAGTAC GAGATTAAAA TACGACCCTA CTTTAACGAG 
TTTCAGGGCC TGGACAGCCG ATCGCAGACA CTCCGCACGC CAGAGGAGGT GCCGAGTGCT 
CCTCCTCGAG CTGTGAATGT GGCCACAGTG AAGCTCAGTA ACAGCTCTAG CATCAGTGTT 
TCATGGCAGC CTCCACCCAC AGACATGCAG AATGGAGTCA TTCAGGAGTA CAAGGTTTGG 
TGTCTGGGCA ATGATTCTCA GACACGCTAC AACATCAATC AGACGGTGGA CGGCAGTGTG 
TTAAGCACTG TACTGAAGGG TCTGCTGCCT GGCGTCCTGT ACCAGGTGGA GGTGGCAGCA 
GTGACCAGTG CAGGAGTGGG CAAACACAGC CAACCCGTAT CTGTTCTTAT CAAATTGCCG 
GCAGAGCAGC CCACAGTCAC AGGTGGAGGC AGTGAATCTG AGGAGAATGT AAGTCTGGCG 
GAGCAGATCA CAGACGTGGT GAAGCAGCCT GCCTTCATCG CAGGCATTGG CGGAGCATGC 
TGGGTAATCC TGATGGGATT CAGCGTGTGG ATCTACTGCC GACGCAAGAA GAGAAAAGAA 
CTCAGCCATT ACACTGCGTC ATTTGCATAC ACACCGGCAG TTGGTTTTCC ACATGGTGAA 
AGTTCTGGAC TTAACGGAAG GCCTGGTATG CTGGGTGCTA ACATGGGAAA TTATCCATGG 
CTGGCTGATT CTTGGCCTAC TACCAATCTT GTGCACAACG GGAAAGACTC AGTGAACTGC 
TGTACATCTA AACACGAAAC ACCGGAGAGA TACTATAACG AGGCGGGAAT CTCTAATTAC 
CTCAATCAAA ATGAGAAGTA CAGCGCAGAC TCCAACGAGG GGCCGATCTA CAGCACCATC 
GACCCCACCA GCGAAGACTT ACGCAGTTTT ACCGCTCAGT ATTCTCAGCA CACCACACCC 
TACGCCAGCA CTCCCATTAT GCCTTTCACC AACCAGGCGC CCTGCAGCCC TGAGCAAGAT 
GGCAGCCACT GGATGACGCA GCCGGGAACC TCCAATGCTC AGTATGCCCA GCCAGACCGA 
TCCAAGACTG AGAACGTAAA ACAACCAAAG CAGAAGTTGA TGGGGAAGCC GGTGAAAACC 
CCATCTCTCA ACTGGATGGA TGCGCTGCCG CCTCCACCCA TCTCTGGAGA TCTGGAGCAG 
TGTGACGAGG AGGATGATAA CGAACTAATA CAAGACATGG GGTCTGATGA GGAAGAGTGG 
TGTCCTCCTC TTCCCGAGCG GTCTTATCTC ATGGACGGCA TGGTGGAGGA CGGTCCAGTT 
CCACCACCAA GAGGAGAAGC TTCTTCACCC ACTACGTCCT ACAGCCACCA GTCCACCGCC 
ACCTTAACCC CTTCTCCCCG AGACGATGGA CACTTCCACG ATATACCACG CTTACACCAG 
CTCGAACAAC CACACATGAC ACGCCGGTTG CCGTGTGGAC CAGTGCCTGT GCCACGAGCA 
CCAAGCCCAC CTCTAACCCA ATCCAACCCA AACCTGTCAG CCAATCAGGA GTGTCCTGAC 
AACACTGTGC CACACCGTTC AGCTCATAAA CGAGACCTGA GCCAAGGAGC TGCACAAAGT 
GTAGAGAACA TCAGCAACAC CACACCAGGT CGAGCGAGGC CCTCCGCAGA CCACACATCG 
TCTGGGCAGA AATCCAAGGT GAAGAAGAAA GCTTCCAAGA GCGCTCAATA CCGGAGAGAT 
ATACTGCAGG GCGATCTGCC TCCTCCACCA GAGCCTCCAC CCGAAGACCA GGATAATTGC 
GCTCATGTAG GGGGCTTCGA ATGCGGGCAG ACGTCCCTCG AGAGAGCCGG CAGCTCCAAT 
TCGTCCATGG AGAGGAGAGA GCAGAGCACA CCCAACCGGA AAAACTCGGC CCATAGACGG 
ATAGACGCAG ATGACGATTT AATTCCTTAC AACAAGCCTA ACTTCCTGTC CCGAGGTCAG 
ATGTCCAGCA ATTGCTCCAC AACAGGAAGT TCATCATCAC GAGGGTCAAC GGGGTCGCGA 
GGGCAAGGAA CGGGCAGGAA GAGAAATGAT GAGATGAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213420.1
CDS	450..4631
Translation	MEFRRTLDALVVVFALICLSQGSRPRLEDAAPRIVEHPSDLIVSKGEPATLNCKAEGRPTPMVEWYKDGERVETDREDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYVCVARNYLGEAVSRNASLEVAILRDDFRQTPSDVVVAAGEPAVMECIPPRGHPEPTISWKRNNVKVNDKDERITIRGGKLMISNTRKSDAGMYVCVGTNMVGEKDSDPAELVVFERPMFVRRPVNQVVLADDTVDFQCEVQGDPAPTIRWKKEEGELPRGRFEIRADNSLRLTQVKAEDEGSYTCLSENSVGKAEASGSLQVHVGPSLPPQIVVRPRDQISAQGRTVTFLCGTKGNPPPAVFWQKEGSQVLLFPSQPPSQSGRFSVSLSGELTITDVHSEDSGYYICQAISVAGSILTKALLEVESTPSDRIPPIIRQGPANQTLAPGTTAQLQCHVMGNPLPSIQWERDGQRILGIDERISLMENGTLQITALQETDSGAYTCVASSLSGETSWSGVLTVKESGGLSASPVSEPYQLPGPPQKPVVTDVTRNSVTLTWQPNAHEGGAAVTSYIIEAFSQSAGSTWQTVADFVKLEKHTVTGLSPNTIYLFIVRAVNAYGLSDPSPISEPVRTQDVSPTGQGVDHRQVQRELGEMSVQLHEPVLLTASSVKISWNVDRQSQYIQGYRLLYRPVGGSWSQQEVKGATERSAVIANLLKGTEYEIKIRPYFNEFQGLDSRSQTLRTPEEVPSAPPRAVNVATVKLSNSSSISVSWQPPPTDMQNGVIQEYKVWCLGNDSQTRYNINQTVDGSVLSTVLKGLLPGVLYQVEVAAVTSAGVGKHSQPVSVLIKLPAEQPTVTGGGSESEENVSLAEQITDVVKQPAFIAGIGGACWVILMGFSVWIYCRRKKRKELSHYTASFAYTPAVGFPHGESSGLNGRPGMLGANMGNYPWLADSWPTTNLVHNGKDSVNCCTSKHETPERYYNEAGISNYLNQNEKYSADSNEGPIYSTIDPTSEDLRSFTAQYSQHTTPYASTPIMPFTNQAPCSPEQDGSHWMTQPGTSNAQYAQPDRSKTENVKQPKQKLMGKPVKTPSLNWMDALPPPPISGDLEQCDEEDDNELIQDMGSDEEEWCPPLPERSYLMDGMVEDGPVPPPRGEASSPTTSYSHQSTATLTPSPRDDGHFHDIPRLHQLEQPHMTRRLPCGPVPVPRAPSPPLTQSNPNLSANQECPDNTVPHRSAHKRDLSQGAAQSVENISNTTPGRARPSADHTSSGQKSKVKKKASKSAQYRRDILQGDLPPPPEPPPEDQDNCAHVGGFECGQTSLERAGSSNSSMERREQSTPNRKNSAHRRIDADDDLIPYNKPNFLSRGQMSSNCSTTGSSSSRGSTGSRGQGTGRKRNDEMR

Target ORF information:

RefSeq Version	XM_017357931.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357931.1

ORF Insert Sequence:

ATGGAGTTTC GCAGGACTTT GGATGCGCTG GTCGTTGTTT TTGCCCTCAT ATGCTTGTCA 
CAAGGATCCC GGCCTAGGTT GGAAGATGCT GCACCCCGGA TTGTTGAACA CCCCTCAGAC 
CTCATTGTTT CCAAAGGTGA GCCTGCAACT TTGAACTGTA AGGCCGAAGG ACGACCGACC 
CCCATGGTGG AGTGGTACAA AGATGGCGAG CGAGTGGAAA CCGACCGGGA GGATCCACGC 
TCCCACCGCA TGCTCCTCCC CAGCGGCTCC CTCTTCTTTC TCCGAATTGT TCACGGAAGG 
CGCAGCAAGC CAGATGAAGG CGTGTACGTG TGCGTAGCAA GGAATTACTT GGGCGAGGCC 
GTCAGTCGTA ATGCCTCTTT GGAGGTCGCA ATTCTCCGGG ATGATTTCCG ACAGACCCCC 
AGTGATGTGG TGGTGGCGGC AGGAGAGCCG GCAGTGATGG AGTGCATCCC TCCACGAGGA 
CACCCAGAAC CCACCATCTC CTGGAAGAGG AACAATGTGA AAGTCAACGA CAAGGACGAG 
AGGATAACGA TCCGAGGAGG GAAGCTGATG ATTTCTAACA CGCGCAAAAG TGATGCCGGG 
ATGTATGTCT GTGTCGGCAC CAATATGGTT GGAGAGAAGG ACAGCGATCC TGCTGAGCTT 
GTAGTGTTTG AGCGGCCCAT GTTTGTGCGC AGACCTGTGA ATCAGGTGGT TCTTGCTGAT 
GATACAGTGG ATTTCCAGTG TGAGGTTCAA GGAGACCCAG CTCCTACCAT ACGCTGGAAG 
AAAGAGGAAG GAGAACTGCC TCGGGGAAGG TTTGAGATCC GTGCCGACAA CAGCCTGCGT 
CTGACTCAGG TGAAGGCTGA AGATGAAGGC TCCTACACCT GTTTATCAGA GAACAGCGTG 
GGCAAAGCTG AAGCATCCGG ATCGCTTCAG GTCCACGTGG GCCCATCTCT TCCTCCTCAG 
ATTGTAGTGC GTCCGCGGGA TCAGATCTCT GCCCAGGGCC GCACTGTCAC CTTTCTCTGC 
GGAACGAAGG GAAATCCCCC TCCTGCTGTT TTCTGGCAGA AAGAAGGCAG TCAGGTCCTG 
CTGTTTCCCA GTCAGCCGCC CTCACAGTCA GGCCGTTTTT CCGTGTCTCT GAGCGGAGAG 
CTTACCATCA CAGATGTGCA CAGCGAGGAT TCTGGCTACT ACATCTGCCA GGCTATCAGT 
GTGGCGGGCA GCATCTTGAC CAAAGCACTG CTGGAGGTGG AAAGCACTCC ATCTGACCGG 
ATCCCTCCCA TCATCCGCCA AGGCCCGGCC AACCAGACGC TAGCTCCGGG CACCACGGCC 
CAATTGCAGT GCCACGTGAT GGGCAACCCT CTTCCGAGCA TCCAATGGGA GCGCGACGGA 
CAACGAATCC TGGGCATCGA CGAGCGCATT AGCCTAATGG AAAACGGCAC TTTGCAAATC 
ACAGCCCTGC AGGAGACGGA CTCCGGGGCC TACACCTGTG TTGCTTCCAG TCTGAGTGGA 
GAGACGAGCT GGAGTGGTGT GCTCACAGTA AAAGAGAGTG GTGGTCTATC AGCATCTCCG 
GTATCAGAGC CCTATCAACT GCCCGGTCCT CCTCAGAAAC CTGTCGTAAC GGATGTAACC 
CGTAACAGTG TCACCCTCAC CTGGCAACCC AACGCACACG AAGGCGGCGC CGCCGTCACC 
TCTTACATCA TCGAGGCCTT CAGTCAATCG GCAGGCAGTA CGTGGCAGAC GGTTGCAGAT 
TTTGTGAAGC TGGAAAAGCA CACGGTCACC GGATTGTCTC CGAACACCAT TTACCTGTTT 
ATTGTGCGAG CCGTCAATGC ATATGGCCTG AGTGACCCAA GCCCAATTTC AGAGCCCGTA 
CGCACACAAG ATGTGAGTCC CACAGGTCAG GGTGTTGACC ATCGTCAGGT GCAGAGGGAG 
CTGGGAGAAA TGTCTGTACA GCTTCACGAA CCCGTGCTGT TGACGGCCTC CAGTGTGAAG 
ATCAGCTGGA ACGTGGACCG TCAGTCGCAG TACATCCAGG GTTATCGGCT GCTTTATCGG 
CCTGTCGGCG GTTCCTGGTC CCAGCAGGAA GTGAAGGGTG CAACAGAGCG TAGCGCCGTC 
ATCGCTAACC TGCTCAAAGG CACCGAGTAC GAGATTAAAA TACGACCCTA CTTTAACGAG 
TTTCAGGGCC TGGACAGCCG ATCGCAGACA CTCCGCACGC CAGAGGAGGT GCCGAGTGCT 
CCTCCTCGAG CTGTGAATGT GGCCACAGTG AAGCTCAGTA ACAGCTCTAG CATCAGTGTT 
TCATGGCAGC CTCCACCCAC AGACATGCAG AATGGAGTCA TTCAGGAGTA CAAGGTTTGG 
TGTCTGGGCA ATGATTCTCA GACACGCTAC AACATCAATC AGACGGTGGA CGGCAGTGTG 
TTAAGCACTG TACTGAAGGG TCTGCTGCCT GGCGTCCTGT ACCAGGTGGA GGTGGCAGCA 
GTGACCAGTG CAGGAGTGGG CAAACACAGC CAACCCGTAT CTGTTCTTAT CAAATTGCCG 
GCAGAGCAGC CCACAGTCAC AGGTGGAGGC AGTGAATCTG AGGAGAATGT AAGTCTGGCG 
GAGCAGATCA CAGACGTGGT GAAGCAGCCT GCCTTCATCG CAGGCATTGG CGGAGCATGC 
TGGGTAATCC TGATGGGATT CAGCGTGTGG ATCTACTGCC GACGCAAGAA GAGAAAAGAA 
CTCAGCCATT ACACTGCGTC ATTTGCATAC ACACCGGCAG TTGGTTTTCC ACATGGTGAA 
AGTTCTGGAC TTAACGGAAG GCCTGGTATG CTGGGTGCTA ACATGGGAAA TTATCCATGG 
CTGGCTGATT CTTGGCCTAC TACCAATCTT GTGCACAACG GGAAAGACTC AGTGAACTGC 
TGTACATCTA AACACGAAAC ACCGGAGAGA TACTATAACG AGGCGGGAAT CTCTAATTAC 
CTCAATCAAA ATGAGAAGTA CAGCGCAGAC TCCAACGAGG GGCCGATCTA CAGCACCATC 
GACCCCACCA GCGAAGACTT ACGCAGTTTT ACCGCTCAGT ATTCTCAGCA CACCACACCC 
TACGCCAGCA CTCCCATTAT GCCTTTCACC AACCAGGCGC CCTGCAGCCC TGAGCAAGAT 
GGCAGCCACT GGATGACGCA GCCGGGAACC TCCAATGCTC AGTATGCCCA GCCAGACCGA 
TCCAAGACTG AGAACGTAAA ACAACCAAAG CAGAAGTTGA TGGGGAAGCC GGTGAAAACC 
CCATCTCTCA ACTGGATGGA TGCGCTGCCG CCTCCACCCA TCTCTGGAGA TCTGGAGCAG 
TGTGACGAGG AGGATGATAA CGAACTAATA CAAGACATGG GGTCTGATGA GGAAGAGTGG 
TGTCCTCCTC TTCCCGAGCG GTCTTATCTC ATGGACGGCA TGGTGGAGGA CGGTCCAGTT 
CCACCACCAA GAGGAGAAGC TTCTTCACCC ACTACGTCCT ACAGCCACCA GTCCACCGCC 
ACCTTAACCC CTTCTCCCCG AGACGATGGA CACTTCCACG ATATACCACG CTTACACCAG 
CTCGAACAAC CACACATGAC ACGCCGGTTG CCGTGTGGAC CAGTGCCTGT GCCACGAGCA 
CCAAGCCCAC CTCTAACCCA ATCCAACCCA AACCTGTCAG CCAATCAGGA GTGTCCTGAC 
AACACTGTGC CACACCGTTC AGCTCATAAA CGAGACCTGA GCCAAGGAGC TGCACAAAGT 
GTAGAGAACA TCAGCAACAC CACACCAGGT CGAGCGAGGC CCTCCGCAGA CCACACATCG 
TCTGGGCAGA AATCCAAGGT GAAGAAGAAA GCTTCCAAGA GCGCTCAATA CCGGAGAGAT 
ATACTGCAGG GCGATCTGCC TCCTCCACCA GAGCCTCCAC CCGAAGACCA GGATAATTGC 
GCTCATGTAG GGGGCTTCGA ATGCGGGCAG ACGTCCCTCG AGAGAGCCGG CAGCTCCAAT 
TCGTCCATGG AGAGGAGAGA GCAGAGCACA CCCAACCGGA AAAACTCGGC CCATAGACGG 
ATAGACGCAG ATGACGATTT AATTCCTTAC AACAAGCCTA ACTTCCTGTC CCGAGGTCAG 
ATGTCCAGCA ATTGCTCCAC AACAGGAAGT TCATCATCAC GAGGGTCAAC GGGGTCGCGA 
GGGCAAGGAA CGGGCAGGAA GAGAAATGAT GAGATGAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_017357931.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213420.1 Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X4, mRNA.

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CloneID	ODa54003
Clone ID Related Accession (Same CDS sequence)	XM_017357931.1 , XM_017357931.2
Accession Version	XM_017357931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTTC GCAGGACTTT GGATGCGCTG GTCGTTGTTT TTGCCCTCAT ATGCTTGTCA 
CAAGGATCCC GGCCTAGGTT GGAAGATGCT GCACCCCGGA TTGTTGAACA CCCCTCAGAC 
CTCATTGTTT CCAAAGGTGA GCCTGCAACT TTGAACTGTA AGGCCGAAGG ACGACCGACC 
CCCATGGTGG AGTGGTACAA AGATGGCGAG CGAGTGGAAA CCGACCGGGA GGATCCACGC 
TCCCACCGCA TGCTCCTCCC CAGCGGCTCC CTCTTCTTTC TCCGAATTGT TCACGGAAGG 
CGCAGCAAGC CAGATGAAGG CGTGTACGTG TGCGTAGCAA GGAATTACTT GGGCGAGGCC 
GTCAGTCGTA ATGCCTCTTT GGAGGTCGCA ATTCTCCGGG ATGATTTCCG ACAGACCCCC 
AGTGATGTGG TGGTGGCGGC AGGAGAGCCG GCAGTGATGG AGTGCATCCC TCCACGAGGA 
CACCCAGAAC CCACCATCTC CTGGAAGAGG AACAATGTGA AAGTCAACGA CAAGGACGAG 
AGGATAACGA TCCGAGGAGG GAAGCTGATG ATTTCTAACA CGCGCAAAAG TGATGCCGGG 
ATGTATGTCT GTGTCGGCAC CAATATGGTT GGAGAGAAGG ACAGCGATCC TGCTGAGCTT 
GTAGTGTTTG AGCGGCCCAT GTTTGTGCGC AGACCTGTGA ATCAGGTGGT TCTTGCTGAT 
GATACAGTGG ATTTCCAGTG TGAGGTTCAA GGAGACCCAG CTCCTACCAT ACGCTGGAAG 
AAAGAGGAAG GAGAACTGCC TCGGGGAAGG TTTGAGATCC GTGCCGACAA CAGCCTGCGT 
CTGACTCAGG TGAAGGCTGA AGATGAAGGC TCCTACACCT GTTTATCAGA GAACAGCGTG 
GGCAAAGCTG AAGCATCCGG ATCGCTTCAG GTCCACGTGG GCCCATCTCT TCCTCCTCAG 
ATTGTAGTGC GTCCGCGGGA TCAGATCTCT GCCCAGGGCC GCACTGTCAC CTTTCTCTGC 
GGAACGAAGG GAAATCCCCC TCCTGCTGTT TTCTGGCAGA AAGAAGGCAG TCAGGTCCTG 
CTGTTTCCCA GTCAGCCGCC CTCACAGTCA GGCCGTTTTT CCGTGTCTCT GAGCGGAGAG 
CTTACCATCA CAGATGTGCA CAGCGAGGAT TCTGGCTACT ACATCTGCCA GGCTATCAGT 
GTGGCGGGCA GCATCTTGAC CAAAGCACTG CTGGAGGTGG AAAGCACTCC ATCTGACCGG 
ATCCCTCCCA TCATCCGCCA AGGCCCGGCC AACCAGACGC TAGCTCCGGG CACCACGGCC 
CAATTGCAGT GCCACGTGAT GGGCAACCCT CTTCCGAGCA TCCAATGGGA GCGCGACGGA 
CAACGAATCC TGGGCATCGA CGAGCGCATT AGCCTAATGG AAAACGGCAC TTTGCAAATC 
ACAGCCCTGC AGGAGACGGA CTCCGGGGCC TACACCTGTG TTGCTTCCAG TCTGAGTGGA 
GAGACGAGCT GGAGTGGTGT GCTCACAGTA AAAGAGAGTG GTGGTCTATC AGCATCTCCG 
GTATCAGAGC CCTATCAACT GCCCGGTCCT CCTCAGAAAC CTGTCGTAAC GGATGTAACC 
CGTAACAGTG TCACCCTCAC CTGGCAACCC AACGCACACG AAGGCGGCGC CGCCGTCACC 
TCTTACATCA TCGAGGCCTT CAGTCAATCG GCAGGCAGTA CGTGGCAGAC GGTTGCAGAT 
TTTGTGAAGC TGGAAAAGCA CACGGTCACC GGATTGTCTC CGAACACCAT TTACCTGTTT 
ATTGTGCGAG CCGTCAATGC ATATGGCCTG AGTGACCCAA GCCCAATTTC AGAGCCCGTA 
CGCACACAAG ATGTGAGTCC CACAGGTCAG GGTGTTGACC ATCGTCAGGT GCAGAGGGAG 
CTGGGAGAAA TGTCTGTACA GCTTCACGAA CCCGTGCTGT TGACGGCCTC CAGTGTGAAG 
ATCAGCTGGA ACGTGGACCG TCAGTCGCAG TACATCCAGG GTTATCGGCT GCTTTATCGG 
CCTGTCGGCG GTTCCTGGTC CCAGCAGGAA GTGAAGGGTG CAACAGAGCG TAGCGCCGTC 
ATCGCTAACC TGCTCAAAGG CACCGAGTAC GAGATTAAAA TACGACCCTA CTTTAACGAG 
TTTCAGGGCC TGGACAGCCG ATCGCAGACA CTCCGCACGC CAGAGGAGGT GCCGAGTGCT 
CCTCCTCGAG CTGTGAATGT GGCCACAGTG AAGCTCAGTA ACAGCTCTAG CATCAGTGTT 
TCATGGCAGC CTCCACCCAC AGACATGCAG AATGGAGTCA TTCAGGAGTA CAAGGTTTGG 
TGTCTGGGCA ATGATTCTCA GACACGCTAC AACATCAATC AGACGGTGGA CGGCAGTGTG 
TTAAGCACTG TACTGAAGGG TCTGCTGCCT GGCGTCCTGT ACCAGGTGGA GGTGGCAGCA 
GTGACCAGTG CAGGAGTGGG CAAACACAGC CAACCCGTAT CTGTTCTTAT CAAATTGCCG 
GCAGAGCAGC CCACAGTCAC AGGTGGAGGC AGTGAATCTG AGGAGAATGT AAGTCTGGCG 
GAGCAGATCA CAGACGTGGT GAAGCAGCCT GCCTTCATCG CAGGCATTGG CGGAGCATGC 
TGGGTAATCC TGATGGGATT CAGCGTGTGG ATCTACTGCC GACGCAAGAA GAGAAAAGAA 
CTCAGCCATT ACACTGCGTC ATTTGCATAC ACACCGGCAG TTGGTTTTCC ACATGGTGAA 
AGTTCTGGAC TTAACGGAAG GCCTGGTATG CTGGGTGCTA ACATGGGAAA TTATCCATGG 
CTGGCTGATT CTTGGCCTAC TACCAATCTT GTGCACAACG GGAAAGACTC AGTGAACTGC 
TGTACATCTA AACACGAAAC ACCGGAGAGA TACTATAACG AGGCGGGAAT CTCTAATTAC 
CTCAATCAAA ATGAGAAGTA CAGCGCAGAC TCCAACGAGG GGCCGATCTA CAGCACCATC 
GACCCCACCA GCGAAGACTT ACGCAGTTTT ACCGCTCAGT ATTCTCAGCA CACCACACCC 
TACGCCAGCA CTCCCATTAT GCCTTTCACC AACCAGGCGC CCTGCAGCCC TGAGCAAGAT 
GGCAGCCACT GGATGACGCA GCCGGGAACC TCCAATGCTC AGTATGCCCA GCCAGACCGA 
TCCAAGACTG AGAACGTAAA ACAACCAAAG CAGAAGTTGA TGGGGAAGCC GGTGAAAACC 
CCATCTCTCA ACTGGATGGA TGCGCTGCCG CCTCCACCCA TCTCTGGAGA TCTGGAGCAG 
TGTGACGAGG AGGATGATAA CGAACTAATA CAAGACATGG GGTCTGATGA GGAAGAGTGG 
TGTCCTCCTC TTCCCGAGCG GTCTTATCTC ATGGACGGCA TGGTGGAGGA CGGTCCAGTT 
CCACCACCAA GAGGAGAAGC TTCTTCACCC ACTACGTCCT ACAGCCACCA GTCCACCGCC 
ACCTTAACCC CTTCTCCCCG AGACGATGGA CACTTCCACG ATATACCACG CTTACACCAG 
CTCGAACAAC CACACATGAC ACGCCGGTTG CCGTGTGGAC CAGTGCCTGT GCCACGAGCA 
CCAAGCCCAC CTCTAACCCA ATCCAACCCA AACCTGTCAG CCAATCAGGA GTGTCCTGAC 
AACACTGTGC CACACCGTTC AGCTCATAAA CGAGACCTGA GCCAAGGAGC TGCACAAAGT 
GTAGAGAACA TCAGCAACAC CACACCAGGT CGAGCGAGGC CCTCCGCAGA CCACACATCG 
TCTGGGCAGA AATCCAAGGT GAAGAAGAAA GCTTCCAAGA GCGCTCAATA CCGGAGAGAT 
ATACTGCAGG GCGATCTGCC TCCTCCACCA GAGCCTCCAC CCGAAGACCA GGATAATTGC 
GCTCATGTAG GGGGCTTCGA ATGCGGGCAG ACGTCCCTCG AGAGAGCCGG CAGCTCCAAT 
TCGTCCATGG AGAGGAGAGA GCAGAGCACA CCCAACCGGA AAAACTCGGC CCATAGACGG 
ATAGACGCAG ATGACGATTT AATTCCTTAC AACAAGCCTA ACTTCCTGTC CCGAGGTCAG 
ATGTCCAGCA ATTGCTCCAC AACAGGAAGT TCATCATCAC GAGGGTCAAC GGGGTCGCGA 
GGGCAAGGAA CGGGCAGGAA GAGAAATGAT GAGATGAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213420.1
CDS	455..4636
Translation	MEFRRTLDALVVVFALICLSQGSRPRLEDAAPRIVEHPSDLIVSKGEPATLNCKAEGRPTPMVEWYKDGERVETDREDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYVCVARNYLGEAVSRNASLEVAILRDDFRQTPSDVVVAAGEPAVMECIPPRGHPEPTISWKRNNVKVNDKDERITIRGGKLMISNTRKSDAGMYVCVGTNMVGEKDSDPAELVVFERPMFVRRPVNQVVLADDTVDFQCEVQGDPAPTIRWKKEEGELPRGRFEIRADNSLRLTQVKAEDEGSYTCLSENSVGKAEASGSLQVHVGPSLPPQIVVRPRDQISAQGRTVTFLCGTKGNPPPAVFWQKEGSQVLLFPSQPPSQSGRFSVSLSGELTITDVHSEDSGYYICQAISVAGSILTKALLEVESTPSDRIPPIIRQGPANQTLAPGTTAQLQCHVMGNPLPSIQWERDGQRILGIDERISLMENGTLQITALQETDSGAYTCVASSLSGETSWSGVLTVKESGGLSASPVSEPYQLPGPPQKPVVTDVTRNSVTLTWQPNAHEGGAAVTSYIIEAFSQSAGSTWQTVADFVKLEKHTVTGLSPNTIYLFIVRAVNAYGLSDPSPISEPVRTQDVSPTGQGVDHRQVQRELGEMSVQLHEPVLLTASSVKISWNVDRQSQYIQGYRLLYRPVGGSWSQQEVKGATERSAVIANLLKGTEYEIKIRPYFNEFQGLDSRSQTLRTPEEVPSAPPRAVNVATVKLSNSSSISVSWQPPPTDMQNGVIQEYKVWCLGNDSQTRYNINQTVDGSVLSTVLKGLLPGVLYQVEVAAVTSAGVGKHSQPVSVLIKLPAEQPTVTGGGSESEENVSLAEQITDVVKQPAFIAGIGGACWVILMGFSVWIYCRRKKRKELSHYTASFAYTPAVGFPHGESSGLNGRPGMLGANMGNYPWLADSWPTTNLVHNGKDSVNCCTSKHETPERYYNEAGISNYLNQNEKYSADSNEGPIYSTIDPTSEDLRSFTAQYSQHTTPYASTPIMPFTNQAPCSPEQDGSHWMTQPGTSNAQYAQPDRSKTENVKQPKQKLMGKPVKTPSLNWMDALPPPPISGDLEQCDEEDDNELIQDMGSDEEEWCPPLPERSYLMDGMVEDGPVPPPRGEASSPTTSYSHQSTATLTPSPRDDGHFHDIPRLHQLEQPHMTRRLPCGPVPVPRAPSPPLTQSNPNLSANQECPDNTVPHRSAHKRDLSQGAAQSVENISNTTPGRARPSADHTSSGQKSKVKKKASKSAQYRRDILQGDLPPPPEPPPEDQDNCAHVGGFECGQTSLERAGSSNSSMERREQSTPNRKNSAHRRIDADDDLIPYNKPNFLSRGQMSSNCSTTGSSSSRGSTGSRGQGTGRKRNDEMR

Target ORF information:

RefSeq Version	XM_017357931.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357931.2

ORF Insert Sequence:

ATGGAGTTTC GCAGGACTTT GGATGCGCTG GTCGTTGTTT TTGCCCTCAT ATGCTTGTCA 
CAAGGATCCC GGCCTAGGTT GGAAGATGCT GCACCCCGGA TTGTTGAACA CCCCTCAGAC 
CTCATTGTTT CCAAAGGTGA GCCTGCAACT TTGAACTGTA AGGCCGAAGG ACGACCGACC 
CCCATGGTGG AGTGGTACAA AGATGGCGAG CGAGTGGAAA CCGACCGGGA GGATCCACGC 
TCCCACCGCA TGCTCCTCCC CAGCGGCTCC CTCTTCTTTC TCCGAATTGT TCACGGAAGG 
CGCAGCAAGC CAGATGAAGG CGTGTACGTG TGCGTAGCAA GGAATTACTT GGGCGAGGCC 
GTCAGTCGTA ATGCCTCTTT GGAGGTCGCA ATTCTCCGGG ATGATTTCCG ACAGACCCCC 
AGTGATGTGG TGGTGGCGGC AGGAGAGCCG GCAGTGATGG AGTGCATCCC TCCACGAGGA 
CACCCAGAAC CCACCATCTC CTGGAAGAGG AACAATGTGA AAGTCAACGA CAAGGACGAG 
AGGATAACGA TCCGAGGAGG GAAGCTGATG ATTTCTAACA CGCGCAAAAG TGATGCCGGG 
ATGTATGTCT GTGTCGGCAC CAATATGGTT GGAGAGAAGG ACAGCGATCC TGCTGAGCTT 
GTAGTGTTTG AGCGGCCCAT GTTTGTGCGC AGACCTGTGA ATCAGGTGGT TCTTGCTGAT 
GATACAGTGG ATTTCCAGTG TGAGGTTCAA GGAGACCCAG CTCCTACCAT ACGCTGGAAG 
AAAGAGGAAG GAGAACTGCC TCGGGGAAGG TTTGAGATCC GTGCCGACAA CAGCCTGCGT 
CTGACTCAGG TGAAGGCTGA AGATGAAGGC TCCTACACCT GTTTATCAGA GAACAGCGTG 
GGCAAAGCTG AAGCATCCGG ATCGCTTCAG GTCCACGTGG GCCCATCTCT TCCTCCTCAG 
ATTGTAGTGC GTCCGCGGGA TCAGATCTCT GCCCAGGGCC GCACTGTCAC CTTTCTCTGC 
GGAACGAAGG GAAATCCCCC TCCTGCTGTT TTCTGGCAGA AAGAAGGCAG TCAGGTCCTG 
CTGTTTCCCA GTCAGCCGCC CTCACAGTCA GGCCGTTTTT CCGTGTCTCT GAGCGGAGAG 
CTTACCATCA CAGATGTGCA CAGCGAGGAT TCTGGCTACT ACATCTGCCA GGCTATCAGT 
GTGGCGGGCA GCATCTTGAC CAAAGCACTG CTGGAGGTGG AAAGCACTCC ATCTGACCGG 
ATCCCTCCCA TCATCCGCCA AGGCCCGGCC AACCAGACGC TAGCTCCGGG CACCACGGCC 
CAATTGCAGT GCCACGTGAT GGGCAACCCT CTTCCGAGCA TCCAATGGGA GCGCGACGGA 
CAACGAATCC TGGGCATCGA CGAGCGCATT AGCCTAATGG AAAACGGCAC TTTGCAAATC 
ACAGCCCTGC AGGAGACGGA CTCCGGGGCC TACACCTGTG TTGCTTCCAG TCTGAGTGGA 
GAGACGAGCT GGAGTGGTGT GCTCACAGTA AAAGAGAGTG GTGGTCTATC AGCATCTCCG 
GTATCAGAGC CCTATCAACT GCCCGGTCCT CCTCAGAAAC CTGTCGTAAC GGATGTAACC 
CGTAACAGTG TCACCCTCAC CTGGCAACCC AACGCACACG AAGGCGGCGC CGCCGTCACC 
TCTTACATCA TCGAGGCCTT CAGTCAATCG GCAGGCAGTA CGTGGCAGAC GGTTGCAGAT 
TTTGTGAAGC TGGAAAAGCA CACGGTCACC GGATTGTCTC CGAACACCAT TTACCTGTTT 
ATTGTGCGAG CCGTCAATGC ATATGGCCTG AGTGACCCAA GCCCAATTTC AGAGCCCGTA 
CGCACACAAG ATGTGAGTCC CACAGGTCAG GGTGTTGACC ATCGTCAGGT GCAGAGGGAG 
CTGGGAGAAA TGTCTGTACA GCTTCACGAA CCCGTGCTGT TGACGGCCTC CAGTGTGAAG 
ATCAGCTGGA ACGTGGACCG TCAGTCGCAG TACATCCAGG GTTATCGGCT GCTTTATCGG 
CCTGTCGGCG GTTCCTGGTC CCAGCAGGAA GTGAAGGGTG CAACAGAGCG TAGCGCCGTC 
ATCGCTAACC TGCTCAAAGG CACCGAGTAC GAGATTAAAA TACGACCCTA CTTTAACGAG 
TTTCAGGGCC TGGACAGCCG ATCGCAGACA CTCCGCACGC CAGAGGAGGT GCCGAGTGCT 
CCTCCTCGAG CTGTGAATGT GGCCACAGTG AAGCTCAGTA ACAGCTCTAG CATCAGTGTT 
TCATGGCAGC CTCCACCCAC AGACATGCAG AATGGAGTCA TTCAGGAGTA CAAGGTTTGG 
TGTCTGGGCA ATGATTCTCA GACACGCTAC AACATCAATC AGACGGTGGA CGGCAGTGTG 
TTAAGCACTG TACTGAAGGG TCTGCTGCCT GGCGTCCTGT ACCAGGTGGA GGTGGCAGCA 
GTGACCAGTG CAGGAGTGGG CAAACACAGC CAACCCGTAT CTGTTCTTAT CAAATTGCCG 
GCAGAGCAGC CCACAGTCAC AGGTGGAGGC AGTGAATCTG AGGAGAATGT AAGTCTGGCG 
GAGCAGATCA CAGACGTGGT GAAGCAGCCT GCCTTCATCG CAGGCATTGG CGGAGCATGC 
TGGGTAATCC TGATGGGATT CAGCGTGTGG ATCTACTGCC GACGCAAGAA GAGAAAAGAA 
CTCAGCCATT ACACTGCGTC ATTTGCATAC ACACCGGCAG TTGGTTTTCC ACATGGTGAA 
AGTTCTGGAC TTAACGGAAG GCCTGGTATG CTGGGTGCTA ACATGGGAAA TTATCCATGG 
CTGGCTGATT CTTGGCCTAC TACCAATCTT GTGCACAACG GGAAAGACTC AGTGAACTGC 
TGTACATCTA AACACGAAAC ACCGGAGAGA TACTATAACG AGGCGGGAAT CTCTAATTAC 
CTCAATCAAA ATGAGAAGTA CAGCGCAGAC TCCAACGAGG GGCCGATCTA CAGCACCATC 
GACCCCACCA GCGAAGACTT ACGCAGTTTT ACCGCTCAGT ATTCTCAGCA CACCACACCC 
TACGCCAGCA CTCCCATTAT GCCTTTCACC AACCAGGCGC CCTGCAGCCC TGAGCAAGAT 
GGCAGCCACT GGATGACGCA GCCGGGAACC TCCAATGCTC AGTATGCCCA GCCAGACCGA 
TCCAAGACTG AGAACGTAAA ACAACCAAAG CAGAAGTTGA TGGGGAAGCC GGTGAAAACC 
CCATCTCTCA ACTGGATGGA TGCGCTGCCG CCTCCACCCA TCTCTGGAGA TCTGGAGCAG 
TGTGACGAGG AGGATGATAA CGAACTAATA CAAGACATGG GGTCTGATGA GGAAGAGTGG 
TGTCCTCCTC TTCCCGAGCG GTCTTATCTC ATGGACGGCA TGGTGGAGGA CGGTCCAGTT 
CCACCACCAA GAGGAGAAGC TTCTTCACCC ACTACGTCCT ACAGCCACCA GTCCACCGCC 
ACCTTAACCC CTTCTCCCCG AGACGATGGA CACTTCCACG ATATACCACG CTTACACCAG 
CTCGAACAAC CACACATGAC ACGCCGGTTG CCGTGTGGAC CAGTGCCTGT GCCACGAGCA 
CCAAGCCCAC CTCTAACCCA ATCCAACCCA AACCTGTCAG CCAATCAGGA GTGTCCTGAC 
AACACTGTGC CACACCGTTC AGCTCATAAA CGAGACCTGA GCCAAGGAGC TGCACAAAGT 
GTAGAGAACA TCAGCAACAC CACACCAGGT CGAGCGAGGC CCTCCGCAGA CCACACATCG 
TCTGGGCAGA AATCCAAGGT GAAGAAGAAA GCTTCCAAGA GCGCTCAATA CCGGAGAGAT 
ATACTGCAGG GCGATCTGCC TCCTCCACCA GAGCCTCCAC CCGAAGACCA GGATAATTGC 
GCTCATGTAG GGGGCTTCGA ATGCGGGCAG ACGTCCCTCG AGAGAGCCGG CAGCTCCAAT 
TCGTCCATGG AGAGGAGAGA GCAGAGCACA CCCAACCGGA AAAACTCGGC CCATAGACGG 
ATAGACGCAG ATGACGATTT AATTCCTTAC AACAAGCCTA ACTTCCTGTC CCGAGGTCAG 
ATGTCCAGCA ATTGCTCCAC AACAGGAAGT TCATCATCAC GAGGGTCAAC GGGGTCGCGA 
GGGCAAGGAA CGGGCAGGAA GAGAAATGAT GAGATGAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( NM_001328416.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315345.1 Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant 1, mRNA.

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CloneID	ODa16202
Clone ID Related Accession (Same CDS sequence)	NM_001328416.1
Accession Version	NM_001328416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-30
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX571852.20 and AL845320.10. On Jun 16, 2016 this sequence version replaced XM_005172639.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). The encoded isoform (1) lacks a predicted signal peptide compared to isoform 2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF304131.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TTCCTCCTCA GATTGTAGTG CGTCCGCGGG ATCAGATCTC TGCCCAGGGC 
CGCACTGTCA CCTTTCTCTG CGGAACGAAG GGAAATCCCC CTCCTGCTGT TTTCTGGCAG 
AAAGAAGGCA GTCAGGTCCT GCTGTTTCCC AGTCAGCCGC CCTCACAGTC AGGCCGTTTT 
TCCGTGTCTC TGAGCGGAGA GCTTACCATC ACAGATGTGC ACAGCGAGGA TTCTGGCTAC 
TACATCTGCC AGGCTATCAG TGTGGCGGGC AGCATCTTGA CCAAAGCACT GCTGGAGGTG 
GAAAGCACTC CATCTGACCG GATCCCTCCC ATCATCCGCC AAGGCCCGGC CAACCAGACG 
CTAGCTCCGG GCACCACGGC CCAATTGCAG TGCCACGTGA TGGGCAACCC TCTTCCGAGC 
ATCCAATGGG AGCGCGACGG ACAACGAATC CTGGGCATCG ACGAGCGCAT TAGCCTAATG 
GAAAACGGCA CTTTGCAAAT CACAGCCCTG CAGGAGACGG ACTCCGGGGC CTACACCTGT 
GTTGCTTCCA GTCTGAGTGG AGAGACGAGC TGGAGTGGTG TGCTCACAGT AAAAGAGAGT 
GGTGGTCTAT CAGCATCTCC GGTATCAGAG CCCTATCAAC TGCCCGGTCC TCCTCAGAAA 
CCTGTCGTAA CGGATGTAAC CCGTAACAGT GTCACCCTCA CCTGGCAACC CAACGCACAC 
GAAGGCGGCG CCGCCGTCAC CTCTTACATC ATCGAGGCCT TCAGTCAATC GGCAGGCAGT 
ACGTGGCAGA CGGTTGCAGA TTTTGTGAAG CTGGAAAAGC ACACGGTCAC CGGATTGTCT 
CCGAACACCA TTTACCTGTT TATTGTGCGA GCCGTCAATG CATATGGCCT GAGTGACCCA 
AGCCCAATTT CAGAGCCCGT ACGCACACAA GATGTGAGTC CCACAGGTCA GGGTGTTGAC 
CATCGTCAGG TGCAGAGGGA GCTGGGAGAA ATGTCTGTAC AGCTTCACGA ACCCGTGCTG 
TTGACGGCCT CCAGTGTGAA GATCAGCTGG AACGTGGACC GTCAGTCGCA GTACATCCAG 
GGTTATCGGC TGCTTTATCG GCCTGTCGGC GGTTCCTGGT CCCAGCAGGA AGTGAAGGGT 
GCAACAGAGC GTAGCGCCGT CATCGCTAAC CTGCTCAAAG GCACCGAGTA CGAGATTAAA 
ATACGACCCT ACTTTAACGA GTTTCAGGGC CTGGACAGCC GATCGCAGAC ACTCCGCACG 
CCAGAGGAGG TGCCGAGTGC TCCTCCTCGA GCTGTGAATG TGGCCACAGT GAAGCTCAGT 
AACAGCTCTA GCATCAGTGT TTCATGGCAG CCTCCACCCA CAGACATGCA GAATGGAGTC 
ATTCAGGAGT ACAAGGTTTG GTGTCTGGGC AATGATTCTC AGACACGCTA CAACATCAAT 
CAGACGGTGG ACGGCAGTGT GTTAAGCACT GTACTGAAGG GTCTGCTGCC TGGCGTCCTG 
TACCAGGTGG AGGTGGCAGC AGTGACCAGT GCAGGAGTGG GCAAACACAG CCAACCCGTA 
TCTGTTCTTA TCAAATTGCC GGCAGAGCAG CCCACAGTCA CAGGTGGAGG CAGTGAATCT 
GAGGAGAATG TAAGTCTGGC GGAGCAGATC ACAGACGTGG TGAAGCAGCC TGCCTTCATC 
GCAGGCATTG GCGGAGCATG CTGGGTAATC CTGATGGGAT TCAGCGTGTG GATCTACTGC 
CGACGCAAGA AGAGAAAAGA ACTCAGCCAT TACACTGCGT CATTTGCATA CACACCGGCA 
GTTGGTTTTC CACATGGTGA AAGTTCTGGA CTTAACGGAA GGCCTGGTAT GCTGGGTGCT 
AACATGGGAA ATTATCCATG GCTGGCTGAT TCTTGGCCTA CTACCAATCT TGTGCACAAC 
GGGAAAGACT CAGTGAACTG CTGTACATCT AAACACGAAA CACCGGAGAG ATACTATAAC 
GAGGCGGGAA TCTCTAATTA CCTCAATCAA AATGAGAAGT ACAGCGCAGA CTCCAACGAG 
GGGCCGATCT ACAGCACCAT CGACCCCACC AGCGAAGACT TACGCAGTTT TACCGCTCAG 
TATTCTCAGC ACACCACACC CTACGCCAGC ACTCCCATTA TGCCTTTCAC CAACCAGGCG 
CCCTGCAGCC CTGAGCAAGA TGGCAGCCAC TGGATGACGC AGCCGGGAAC CTCCAATGCT 
CAGTATGCCC AGCCAGACCG ATCCAAGACT GAGAACGTAA AACAACCAAA GCAGAAGTTG 
ATGGGGAAGC CGGTGAAAAC CCCATCTCTC AACTGGATGG ATGCGCTGCC GCCTCCACCC 
ATCTCTGGAG ATCTGGAGCA GTGTGACGAG GAGGATGATA ACGAACTAAT ACAAGACATG 
GGGTCTGATG AGGAAGAGTG GTGTCCTCCT CTTCCCGAGC GGTCTTATCT CATGGACGGC 
ATGGTGGAGG ACGGTCCAGT TCCACCACCA AGAGGAGAAG CTTCTTCACC CACTACGTCC 
TACAGCCACC AGTCCACCGC CACCTTAACC CCTTCTCCCC GAGACGATGG ACACTTCCAC 
GATATACCAC GCTTACACCA GCTCGAACAA CCACACATGA CACGCCGGTT GCCGTGTGGA 
CCAGTGCCTG TGCCACGAGC ACCAAGCCCA CCTCTAACCC AATCCAACCC AAACCTGTCA 
GCCAATCAGG AGTGTCCTGA CAACACTGTG CCACACCGTT CAGCTCATAA ACGAGACCTG 
AGCCAAGGAG CTGCACAAAG TGTAGAGAAC ATCAGCAACA CCACACCAGG TCGAGCGAGG 
CCCTCCGCAG ACCACACATC GTCTGGGCAG AAATCCAAGG TGAAGAAGAA AGCTTCCAAG 
AGCGCTCAAT ACCGGAGAGA TATACTGCAG GGCGATCTGC CTCCTCCACC AGAGCCTCCA 
CCCGAAGACC AGGATAATTG CGCTCATGTA GGGGGCTTCG AATGCGGGCA GACGTCCCTC 
GAGAGAGCCG GCAGCTCCAA TTCGTCCATG GAGAGGAGAG AGCAGAGCAC ACCCAACCGG 
AAAAACTCGG CCCATAGACG GATAGACGAT GACGATTTAA TTCCTTACAA CAAGCCTAAC 
TTCCTGTCCC GAGGTCAGAT GTCCAGCAAT TGCTCCACAA CAGGAAGTTC ATCATCACGA 
GGGTCAACGG GGTCGCGAGG GCAAGGAACG GGCAGGAAGA GAAATGATGA GATGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315345.1
CDS	355..4614
Translation	MLRYLIKTLLQMNLFTDTIRDATNGTDLFFNISSIPSFNSSLLDWANFTGLNGSRPRLEDAAPRIVEHPSDLIVSKGEPATLNCKAEGRPTPMVEWYKDGERVETDREDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYVCVARNYLGEAVSRNASLEVAILRDDFRQTPSDVVVAAGEPAVMECIPPRGHPEPTISWKRNNVKVNDKDERITIRGGKLMISNTRKSDAGMYVCVGTNMVGEKDSDPAELVVFERPMFVRRPVNQVVLADDTVDFQCEVQGDPAPTIRWKKEEGELPRGRFEIRADNSLRLTQVKAEDEGSYTCLSENSVGKAEASGSLQVHVPPQIVVRPRDQISAQGRTVTFLCGTKGNPPPAVFWQKEGSQVLLFPSQPPSQSGRFSVSLSGELTITDVHSEDSGYYICQAISVAGSILTKALLEVESTPSDRIPPIIRQGPANQTLAPGTTAQLQCHVMGNPLPSIQWERDGQRILGIDERISLMENGTLQITALQETDSGAYTCVASSLSGETSWSGVLTVKESGGLSASPVSEPYQLPGPPQKPVVTDVTRNSVTLTWQPNAHEGGAAVTSYIIEAFSQSAGSTWQTVADFVKLEKHTVTGLSPNTIYLFIVRAVNAYGLSDPSPISEPVRTQDVSPTGQGVDHRQVQRELGEMSVQLHEPVLLTASSVKISWNVDRQSQYIQGYRLLYRPVGGSWSQQEVKGATERSAVIANLLKGTEYEIKIRPYFNEFQGLDSRSQTLRTPEEVPSAPPRAVNVATVKLSNSSSISVSWQPPPTDMQNGVIQEYKVWCLGNDSQTRYNINQTVDGSVLSTVLKGLLPGVLYQVEVAAVTSAGVGKHSQPVSVLIKLPAEQPTVTGGGSESEENVSLAEQITDVVKQPAFIAGIGGACWVILMGFSVWIYCRRKKRKELSHYTASFAYTPAVGFPHGESSGLNGRPGMLGANMGNYPWLADSWPTTNLVHNGKDSVNCCTSKHETPERYYNEAGISNYLNQNEKYSADSNEGPIYSTIDPTSEDLRSFTAQYSQHTTPYASTPIMPFTNQAPCSPEQDGSHWMTQPGTSNAQYAQPDRSKTENVKQPKQKLMGKPVKTPSLNWMDALPPPPISGDLEQCDEEDDNELIQDMGSDEEEWCPPLPERSYLMDGMVEDGPVPPPRGEASSPTTSYSHQSTATLTPSPRDDGHFHDIPRLHQLEQPHMTRRLPCGPVPVPRAPSPPLTQSNPNLSANQECPDNTVPHRSAHKRDLSQGAAQSVENISNTTPGRARPSADHTSSGQKSKVKKKASKSAQYRRDILQGDLPPPPEPPPEDQDNCAHVGGFECGQTSLERAGSSNSSMERREQSTPNRKNSAHRRIDDDDLIPYNKPNFLSRGQMSSNCSTTGSSSSRGSTGSRGQGTGRKRNDEMR

Target ORF information:

RefSeq Version	NM_001328416.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328416.1

ORF Insert Sequence:

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TTCCTCCTCA GATTGTAGTG CGTCCGCGGG ATCAGATCTC TGCCCAGGGC 
CGCACTGTCA CCTTTCTCTG CGGAACGAAG GGAAATCCCC CTCCTGCTGT TTTCTGGCAG 
AAAGAAGGCA GTCAGGTCCT GCTGTTTCCC AGTCAGCCGC CCTCACAGTC AGGCCGTTTT 
TCCGTGTCTC TGAGCGGAGA GCTTACCATC ACAGATGTGC ACAGCGAGGA TTCTGGCTAC 
TACATCTGCC AGGCTATCAG TGTGGCGGGC AGCATCTTGA CCAAAGCACT GCTGGAGGTG 
GAAAGCACTC CATCTGACCG GATCCCTCCC ATCATCCGCC AAGGCCCGGC CAACCAGACG 
CTAGCTCCGG GCACCACGGC CCAATTGCAG TGCCACGTGA TGGGCAACCC TCTTCCGAGC 
ATCCAATGGG AGCGCGACGG ACAACGAATC CTGGGCATCG ACGAGCGCAT TAGCCTAATG 
GAAAACGGCA CTTTGCAAAT CACAGCCCTG CAGGAGACGG ACTCCGGGGC CTACACCTGT 
GTTGCTTCCA GTCTGAGTGG AGAGACGAGC TGGAGTGGTG TGCTCACAGT AAAAGAGAGT 
GGTGGTCTAT CAGCATCTCC GGTATCAGAG CCCTATCAAC TGCCCGGTCC TCCTCAGAAA 
CCTGTCGTAA CGGATGTAAC CCGTAACAGT GTCACCCTCA CCTGGCAACC CAACGCACAC 
GAAGGCGGCG CCGCCGTCAC CTCTTACATC ATCGAGGCCT TCAGTCAATC GGCAGGCAGT 
ACGTGGCAGA CGGTTGCAGA TTTTGTGAAG CTGGAAAAGC ACACGGTCAC CGGATTGTCT 
CCGAACACCA TTTACCTGTT TATTGTGCGA GCCGTCAATG CATATGGCCT GAGTGACCCA 
AGCCCAATTT CAGAGCCCGT ACGCACACAA GATGTGAGTC CCACAGGTCA GGGTGTTGAC 
CATCGTCAGG TGCAGAGGGA GCTGGGAGAA ATGTCTGTAC AGCTTCACGA ACCCGTGCTG 
TTGACGGCCT CCAGTGTGAA GATCAGCTGG AACGTGGACC GTCAGTCGCA GTACATCCAG 
GGTTATCGGC TGCTTTATCG GCCTGTCGGC GGTTCCTGGT CCCAGCAGGA AGTGAAGGGT 
GCAACAGAGC GTAGCGCCGT CATCGCTAAC CTGCTCAAAG GCACCGAGTA CGAGATTAAA 
ATACGACCCT ACTTTAACGA GTTTCAGGGC CTGGACAGCC GATCGCAGAC ACTCCGCACG 
CCAGAGGAGG TGCCGAGTGC TCCTCCTCGA GCTGTGAATG TGGCCACAGT GAAGCTCAGT 
AACAGCTCTA GCATCAGTGT TTCATGGCAG CCTCCACCCA CAGACATGCA GAATGGAGTC 
ATTCAGGAGT ACAAGGTTTG GTGTCTGGGC AATGATTCTC AGACACGCTA CAACATCAAT 
CAGACGGTGG ACGGCAGTGT GTTAAGCACT GTACTGAAGG GTCTGCTGCC TGGCGTCCTG 
TACCAGGTGG AGGTGGCAGC AGTGACCAGT GCAGGAGTGG GCAAACACAG CCAACCCGTA 
TCTGTTCTTA TCAAATTGCC GGCAGAGCAG CCCACAGTCA CAGGTGGAGG CAGTGAATCT 
GAGGAGAATG TAAGTCTGGC GGAGCAGATC ACAGACGTGG TGAAGCAGCC TGCCTTCATC 
GCAGGCATTG GCGGAGCATG CTGGGTAATC CTGATGGGAT TCAGCGTGTG GATCTACTGC 
CGACGCAAGA AGAGAAAAGA ACTCAGCCAT TACACTGCGT CATTTGCATA CACACCGGCA 
GTTGGTTTTC CACATGGTGA AAGTTCTGGA CTTAACGGAA GGCCTGGTAT GCTGGGTGCT 
AACATGGGAA ATTATCCATG GCTGGCTGAT TCTTGGCCTA CTACCAATCT TGTGCACAAC 
GGGAAAGACT CAGTGAACTG CTGTACATCT AAACACGAAA CACCGGAGAG ATACTATAAC 
GAGGCGGGAA TCTCTAATTA CCTCAATCAA AATGAGAAGT ACAGCGCAGA CTCCAACGAG 
GGGCCGATCT ACAGCACCAT CGACCCCACC AGCGAAGACT TACGCAGTTT TACCGCTCAG 
TATTCTCAGC ACACCACACC CTACGCCAGC ACTCCCATTA TGCCTTTCAC CAACCAGGCG 
CCCTGCAGCC CTGAGCAAGA TGGCAGCCAC TGGATGACGC AGCCGGGAAC CTCCAATGCT 
CAGTATGCCC AGCCAGACCG ATCCAAGACT GAGAACGTAA AACAACCAAA GCAGAAGTTG 
ATGGGGAAGC CGGTGAAAAC CCCATCTCTC AACTGGATGG ATGCGCTGCC GCCTCCACCC 
ATCTCTGGAG ATCTGGAGCA GTGTGACGAG GAGGATGATA ACGAACTAAT ACAAGACATG 
GGGTCTGATG AGGAAGAGTG GTGTCCTCCT CTTCCCGAGC GGTCTTATCT CATGGACGGC 
ATGGTGGAGG ACGGTCCAGT TCCACCACCA AGAGGAGAAG CTTCTTCACC CACTACGTCC 
TACAGCCACC AGTCCACCGC CACCTTAACC CCTTCTCCCC GAGACGATGG ACACTTCCAC 
GATATACCAC GCTTACACCA GCTCGAACAA CCACACATGA CACGCCGGTT GCCGTGTGGA 
CCAGTGCCTG TGCCACGAGC ACCAAGCCCA CCTCTAACCC AATCCAACCC AAACCTGTCA 
GCCAATCAGG AGTGTCCTGA CAACACTGTG CCACACCGTT CAGCTCATAA ACGAGACCTG 
AGCCAAGGAG CTGCACAAAG TGTAGAGAAC ATCAGCAACA CCACACCAGG TCGAGCGAGG 
CCCTCCGCAG ACCACACATC GTCTGGGCAG AAATCCAAGG TGAAGAAGAA AGCTTCCAAG 
AGCGCTCAAT ACCGGAGAGA TATACTGCAG GGCGATCTGC CTCCTCCACC AGAGCCTCCA 
CCCGAAGACC AGGATAATTG CGCTCATGTA GGGGGCTTCG AATGCGGGCA GACGTCCCTC 
GAGAGAGCCG GCAGCTCCAA TTCGTCCATG GAGAGGAGAG AGCAGAGCAC ACCCAACCGG 
AAAAACTCGG CCCATAGACG GATAGACGAT GACGATTTAA TTCCTTACAA CAAGCCTAAC 
TTCCTGTCCC GAGGTCAGAT GTCCAGCAAT TGCTCCACAA CAGGAAGTTC ATCATCACGA 
GGGTCAACGG GGTCGCGAGG GCAAGGAACG GGCAGGAAGA GAAATGATGA GATGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_005172636.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172693.1 Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16199
Clone ID Related Accession (Same CDS sequence)	XM_005172636.4 , XM_005172636.3
Accession Version	XM_005172636.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172636.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TGGGCCCATC TCTTCCTCCT CAGATTGTAG TGCGTCCGCG GGATCAGATC 
TCTGCCCAGG GCCGCACTGT CACCTTTCTC TGCGGAACGA AGGGAAATCC CCCTCCTGCT 
GTTTTCTGGC AGAAAGAAGG CAGTCAGGTC CTGCTGTTTC CCAGTCAGCC GCCCTCACAG 
TCAGGCCGTT TTTCCGTGTC TCTGAGCGGA GAGCTTACCA TCACAGATGT GCACAGCGAG 
GATTCTGGCT ACTACATCTG CCAGGCTATC AGTGTGGCGG GCAGCATCTT GACCAAAGCA 
CTGCTGGAGG TGGAAAGCAC TCCATCTGAC CGGATCCCTC CCATCATCCG CCAAGGCCCG 
GCCAACCAGA CGCTAGCTCC GGGCACCACG GCCCAATTGC AGTGCCACGT GATGGGCAAC 
CCTCTTCCGA GCATCCAATG GGAGCGCGAC GGACAACGAA TCCTGGGCAT CGACGAGCGC 
ATTAGCCTAA TGGAAAACGG CACTTTGCAA ATCACAGCCC TGCAGGAGAC GGACTCCGGG 
GCCTACACCT GTGTTGCTTC CAGTCTGAGT GGAGAGACGA GCTGGAGTGG TGTGCTCACA 
GTAAAAGAGA GTGGTGGTCT ATCAGCATCT CCGGTATCAG AGCCCTATCA ACTGCCCGGT 
CCTCCTCAGA AACCTGTCGT AACGGATGTA ACCCGTAACA GTGTCACCCT CACCTGGCAA 
CCCAACGCAC ACGAAGGCGG CGCCGCCGTC ACCTCTTACA TCATCGAGGC CTTCAGTCAA 
TCGGCAGGCA GTACGTGGCA GACGGTTGCA GATTTTGTGA AGCTGGAAAA GCACACGGTC 
ACCGGATTGT CTCCGAACAC CATTTACCTG TTTATTGTGC GAGCCGTCAA TGCATATGGC 
CTGAGTGACC CAAGCCCAAT TTCAGAGCCC GTACGCACAC AAGATGTGAG TCCCACAGGT 
CAGGGTGTTG ACCATCGTCA GGTGCAGAGG GAGCTGGGAG AAATGTCTGT ACAGCTTCAC 
GAACCCGTGC TGTTGACGGC CTCCAGTGTG AAGATCAGCT GGAACGTGGA CCGTCAGTCG 
CAGTACATCC AGGGTTATCG GCTGCTTTAT CGGCCTGTCG GCGGTTCCTG GTCCCAGCAG 
GAAGTGAAGG GTGCAACAGA GCGTAGCGCC GTCATCGCTA ACCTGCTCAA AGGCACCGAG 
TACGAGATTA AAATACGACC CTACTTTAAC GAGTTTCAGG GCCTGGACAG CCGATCGCAG 
ACACTCCGCA CGCCAGAGGA GGTGCCGAGT GCTCCTCCTC GAGCTGTGAA TGTGGCCACA 
GTGAAGCTCA GTAACAGCTC TAGCATCAGT GTTTCATGGC AGCCTCCACC CACAGACATG 
CAGAATGGAG TCATTCAGGA GTACAAGGTT TGGTGTCTGG GCAATGATTC TCAGACACGC 
TACAACATCA ATCAGACGGT GGACGGCAGT GTGTTAAGCA CTGTACTGAA GGGTCTGCTG 
CCTGGCGTCC TGTACCAGGT GGAGGTGGCA GCAGTGACCA GTGCAGGAGT GGGCAAACAC 
AGCCAACCCG TATCTGTTCT TATCAAATTG CCGGCAGAGC AGCCCACAGT CACAGGTGGA 
GGCAGTGAAT CTGAGGAGAA TGTAAGTCTG GCGGAGCAGA TCACAGACGT GGTGAAGCAG 
CCTGCCTTCA TCGCAGGCAT TGGCGGAGCA TGCTGGGTAA TCCTGATGGG ATTCAGCGTG 
TGGATCTACT GCCGACGCAA GAAGAGAAAA GAACTCAGCC ATTACACTGC GTCATTTGCA 
TACACACCGG CAGTTGGTTT TCCACATGGT GAAAGTTCTG GACTTAACGG AAGGCCTGGT 
ATGCTGGGTG CTAACATGGG AAATTATCCA TGGCTGGCTG ATTCTTGGCC TACTACCAAT 
CTTGTGCACA ACGGGAAAGA CTCAGTGAAC TGCTGTACAT CTAAACACGA AACACCGGAG 
AGATACTATA ACGAGGCGGG AATCTCTAAT TACCTCAATC AAAATGAGAA GTACAGCGCA 
GACTCCAACG AGGGGCCGAT CTACAGCACC ATCGACCCCA CCAGCGAAGA CTTACGCAGT 
TTTACCGCTC AGTATTCTCA GCACACCACA CCCTACGCCA GCACTCCCAT TATGCCTTTC 
ACCAACCAGG CGCCCTGCAG CCCTGAGCAA GATGGCAGCC ACTGGATGAC GCAGCCGGGA 
ACCTCCAATG CTCAGTATGC CCAGCCAGAC CGATCCAAGA CTGAGAACGT AAAACAACCA 
AAGCAGAAGT TGATGGGGAA GCCGGTGAAA ACCCCATCTC TCAACTGGAT GGATGCGCTG 
CCGCCTCCAC CCATCTCTGG AGATCTGGAG CAGTGTGACG AGGAGGATGA TAACGAACTA 
ATACAAGACA TGGGGTCTGA TGAGGAAGAG TGGTGTCCTC CTCTTCCCGA GCGGTCTTAT 
CTCATGGACG GCATGGTGGA GGACGGTCCA GTTCCACCAC CAAGAGGAGA AGCTTCTTCA 
CCCACTACGT CCTACAGCCA CCAGTCCACC GCCACCTTAA CCCCTTCTCC CCGAGACGAT 
GGACACTTCC ACGATATACC ACGCTTACAC CAGCTCGAAC AACCACACAT GACACGCCGG 
TTGCCGTGTG GACCAGTGCC TGTGCCACGA GCACCAAGCC CACCTCTAAC CCAATCCAAC 
CCAAACCTGT CAGCCAATCA GGAGTGTCCT GACAACACTG TGCCACACCG TTCAGCTCAT 
AAACGAGACC TGAGCCAAGG AGCTGCACAA AGTGTAGAGA ACATCAGCAA CACCACACCA 
GGTCGAGCGA GGCCCTCCGC AGACCACACA TCGTCTGGGC AGAAATCCAA GGTGAAGAAG 
AAAGCTTCCA AGAGCGCTCA ATACCGGAGA GATATACTGC AGGGCGATCT GCCTCCTCCA 
CCAGAGCCTC CACCCGAAGA CCAGGATAAT TGCGCTCATG TAGGGGGCTT CGAATGCGGG 
CAGACGTCCC TCGAGAGAGC CGGCAGCTCC AATTCGTCCA TGGAGAGGAG AGAGCAGAGC 
ACACCCAACC GGAAAAACTC GGCCCATAGA CGGATAGACG CAGATGACGA TTTAATTCCT 
TACAACAAGC CTAACTTCCT GTCCCGAGGT CAGATGTCCA GCAATTGCTC CACAACAGGA 
AGTTCATCAT CACGAGGGTC AACGGGGTCG CGAGGGCAAG GAACGGGCAG GAAGAGAAAT 
GATGAGATGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172693.1
CDS	430..4704
Translation	MLRYLIKTLLQMNLFTDTIRDATNGTDLFFNISSIPSFNSSLLDWANFTGLNGSRPRLEDAAPRIVEHPSDLIVSKGEPATLNCKAEGRPTPMVEWYKDGERVETDREDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYVCVARNYLGEAVSRNASLEVAILRDDFRQTPSDVVVAAGEPAVMECIPPRGHPEPTISWKRNNVKVNDKDERITIRGGKLMISNTRKSDAGMYVCVGTNMVGEKDSDPAELVVFERPMFVRRPVNQVVLADDTVDFQCEVQGDPAPTIRWKKEEGELPRGRFEIRADNSLRLTQVKAEDEGSYTCLSENSVGKAEASGSLQVHVGPSLPPQIVVRPRDQISAQGRTVTFLCGTKGNPPPAVFWQKEGSQVLLFPSQPPSQSGRFSVSLSGELTITDVHSEDSGYYICQAISVAGSILTKALLEVESTPSDRIPPIIRQGPANQTLAPGTTAQLQCHVMGNPLPSIQWERDGQRILGIDERISLMENGTLQITALQETDSGAYTCVASSLSGETSWSGVLTVKESGGLSASPVSEPYQLPGPPQKPVVTDVTRNSVTLTWQPNAHEGGAAVTSYIIEAFSQSAGSTWQTVADFVKLEKHTVTGLSPNTIYLFIVRAVNAYGLSDPSPISEPVRTQDVSPTGQGVDHRQVQRELGEMSVQLHEPVLLTASSVKISWNVDRQSQYIQGYRLLYRPVGGSWSQQEVKGATERSAVIANLLKGTEYEIKIRPYFNEFQGLDSRSQTLRTPEEVPSAPPRAVNVATVKLSNSSSISVSWQPPPTDMQNGVIQEYKVWCLGNDSQTRYNINQTVDGSVLSTVLKGLLPGVLYQVEVAAVTSAGVGKHSQPVSVLIKLPAEQPTVTGGGSESEENVSLAEQITDVVKQPAFIAGIGGACWVILMGFSVWIYCRRKKRKELSHYTASFAYTPAVGFPHGESSGLNGRPGMLGANMGNYPWLADSWPTTNLVHNGKDSVNCCTSKHETPERYYNEAGISNYLNQNEKYSADSNEGPIYSTIDPTSEDLRSFTAQYSQHTTPYASTPIMPFTNQAPCSPEQDGSHWMTQPGTSNAQYAQPDRSKTENVKQPKQKLMGKPVKTPSLNWMDALPPPPISGDLEQCDEEDDNELIQDMGSDEEEWCPPLPERSYLMDGMVEDGPVPPPRGEASSPTTSYSHQSTATLTPSPRDDGHFHDIPRLHQLEQPHMTRRLPCGPVPVPRAPSPPLTQSNPNLSANQECPDNTVPHRSAHKRDLSQGAAQSVENISNTTPGRARPSADHTSSGQKSKVKKKASKSAQYRRDILQGDLPPPPEPPPEDQDNCAHVGGFECGQTSLERAGSSNSSMERREQSTPNRKNSAHRRIDADDDLIPYNKPNFLSRGQMSSNCSTTGSSSSRGSTGSRGQGTGRKRNDEMR

Target ORF information:

RefSeq Version	XM_005172636.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172636.4

ORF Insert Sequence:

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TGGGCCCATC TCTTCCTCCT CAGATTGTAG TGCGTCCGCG GGATCAGATC 
TCTGCCCAGG GCCGCACTGT CACCTTTCTC TGCGGAACGA AGGGAAATCC CCCTCCTGCT 
GTTTTCTGGC AGAAAGAAGG CAGTCAGGTC CTGCTGTTTC CCAGTCAGCC GCCCTCACAG 
TCAGGCCGTT TTTCCGTGTC TCTGAGCGGA GAGCTTACCA TCACAGATGT GCACAGCGAG 
GATTCTGGCT ACTACATCTG CCAGGCTATC AGTGTGGCGG GCAGCATCTT GACCAAAGCA 
CTGCTGGAGG TGGAAAGCAC TCCATCTGAC CGGATCCCTC CCATCATCCG CCAAGGCCCG 
GCCAACCAGA CGCTAGCTCC GGGCACCACG GCCCAATTGC AGTGCCACGT GATGGGCAAC 
CCTCTTCCGA GCATCCAATG GGAGCGCGAC GGACAACGAA TCCTGGGCAT CGACGAGCGC 
ATTAGCCTAA TGGAAAACGG CACTTTGCAA ATCACAGCCC TGCAGGAGAC GGACTCCGGG 
GCCTACACCT GTGTTGCTTC CAGTCTGAGT GGAGAGACGA GCTGGAGTGG TGTGCTCACA 
GTAAAAGAGA GTGGTGGTCT ATCAGCATCT CCGGTATCAG AGCCCTATCA ACTGCCCGGT 
CCTCCTCAGA AACCTGTCGT AACGGATGTA ACCCGTAACA GTGTCACCCT CACCTGGCAA 
CCCAACGCAC ACGAAGGCGG CGCCGCCGTC ACCTCTTACA TCATCGAGGC CTTCAGTCAA 
TCGGCAGGCA GTACGTGGCA GACGGTTGCA GATTTTGTGA AGCTGGAAAA GCACACGGTC 
ACCGGATTGT CTCCGAACAC CATTTACCTG TTTATTGTGC GAGCCGTCAA TGCATATGGC 
CTGAGTGACC CAAGCCCAAT TTCAGAGCCC GTACGCACAC AAGATGTGAG TCCCACAGGT 
CAGGGTGTTG ACCATCGTCA GGTGCAGAGG GAGCTGGGAG AAATGTCTGT ACAGCTTCAC 
GAACCCGTGC TGTTGACGGC CTCCAGTGTG AAGATCAGCT GGAACGTGGA CCGTCAGTCG 
CAGTACATCC AGGGTTATCG GCTGCTTTAT CGGCCTGTCG GCGGTTCCTG GTCCCAGCAG 
GAAGTGAAGG GTGCAACAGA GCGTAGCGCC GTCATCGCTA ACCTGCTCAA AGGCACCGAG 
TACGAGATTA AAATACGACC CTACTTTAAC GAGTTTCAGG GCCTGGACAG CCGATCGCAG 
ACACTCCGCA CGCCAGAGGA GGTGCCGAGT GCTCCTCCTC GAGCTGTGAA TGTGGCCACA 
GTGAAGCTCA GTAACAGCTC TAGCATCAGT GTTTCATGGC AGCCTCCACC CACAGACATG 
CAGAATGGAG TCATTCAGGA GTACAAGGTT TGGTGTCTGG GCAATGATTC TCAGACACGC 
TACAACATCA ATCAGACGGT GGACGGCAGT GTGTTAAGCA CTGTACTGAA GGGTCTGCTG 
CCTGGCGTCC TGTACCAGGT GGAGGTGGCA GCAGTGACCA GTGCAGGAGT GGGCAAACAC 
AGCCAACCCG TATCTGTTCT TATCAAATTG CCGGCAGAGC AGCCCACAGT CACAGGTGGA 
GGCAGTGAAT CTGAGGAGAA TGTAAGTCTG GCGGAGCAGA TCACAGACGT GGTGAAGCAG 
CCTGCCTTCA TCGCAGGCAT TGGCGGAGCA TGCTGGGTAA TCCTGATGGG ATTCAGCGTG 
TGGATCTACT GCCGACGCAA GAAGAGAAAA GAACTCAGCC ATTACACTGC GTCATTTGCA 
TACACACCGG CAGTTGGTTT TCCACATGGT GAAAGTTCTG GACTTAACGG AAGGCCTGGT 
ATGCTGGGTG CTAACATGGG AAATTATCCA TGGCTGGCTG ATTCTTGGCC TACTACCAAT 
CTTGTGCACA ACGGGAAAGA CTCAGTGAAC TGCTGTACAT CTAAACACGA AACACCGGAG 
AGATACTATA ACGAGGCGGG AATCTCTAAT TACCTCAATC AAAATGAGAA GTACAGCGCA 
GACTCCAACG AGGGGCCGAT CTACAGCACC ATCGACCCCA CCAGCGAAGA CTTACGCAGT 
TTTACCGCTC AGTATTCTCA GCACACCACA CCCTACGCCA GCACTCCCAT TATGCCTTTC 
ACCAACCAGG CGCCCTGCAG CCCTGAGCAA GATGGCAGCC ACTGGATGAC GCAGCCGGGA 
ACCTCCAATG CTCAGTATGC CCAGCCAGAC CGATCCAAGA CTGAGAACGT AAAACAACCA 
AAGCAGAAGT TGATGGGGAA GCCGGTGAAA ACCCCATCTC TCAACTGGAT GGATGCGCTG 
CCGCCTCCAC CCATCTCTGG AGATCTGGAG CAGTGTGACG AGGAGGATGA TAACGAACTA 
ATACAAGACA TGGGGTCTGA TGAGGAAGAG TGGTGTCCTC CTCTTCCCGA GCGGTCTTAT 
CTCATGGACG GCATGGTGGA GGACGGTCCA GTTCCACCAC CAAGAGGAGA AGCTTCTTCA 
CCCACTACGT CCTACAGCCA CCAGTCCACC GCCACCTTAA CCCCTTCTCC CCGAGACGAT 
GGACACTTCC ACGATATACC ACGCTTACAC CAGCTCGAAC AACCACACAT GACACGCCGG 
TTGCCGTGTG GACCAGTGCC TGTGCCACGA GCACCAAGCC CACCTCTAAC CCAATCCAAC 
CCAAACCTGT CAGCCAATCA GGAGTGTCCT GACAACACTG TGCCACACCG TTCAGCTCAT 
AAACGAGACC TGAGCCAAGG AGCTGCACAA AGTGTAGAGA ACATCAGCAA CACCACACCA 
GGTCGAGCGA GGCCCTCCGC AGACCACACA TCGTCTGGGC AGAAATCCAA GGTGAAGAAG 
AAAGCTTCCA AGAGCGCTCA ATACCGGAGA GATATACTGC AGGGCGATCT GCCTCCTCCA 
CCAGAGCCTC CACCCGAAGA CCAGGATAAT TGCGCTCATG TAGGGGGCTT CGAATGCGGG 
CAGACGTCCC TCGAGAGAGC CGGCAGCTCC AATTCGTCCA TGGAGAGGAG AGAGCAGAGC 
ACACCCAACC GGAAAAACTC GGCCCATAGA CGGATAGACG CAGATGACGA TTTAATTCCT 
TACAACAAGC CTAACTTCCT GTCCCGAGGT CAGATGTCCA GCAATTGCTC CACAACAGGA 
AGTTCATCAT CACGAGGGTC AACGGGGTCG CGAGGGCAAG GAACGGGCAG GAAGAGAAAT 
GATGAGATGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_005172636.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172693.1 Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16199
Clone ID Related Accession (Same CDS sequence)	XM_005172636.4 , XM_005172636.3
Accession Version	XM_005172636.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172636.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TGGGCCCATC TCTTCCTCCT CAGATTGTAG TGCGTCCGCG GGATCAGATC 
TCTGCCCAGG GCCGCACTGT CACCTTTCTC TGCGGAACGA AGGGAAATCC CCCTCCTGCT 
GTTTTCTGGC AGAAAGAAGG CAGTCAGGTC CTGCTGTTTC CCAGTCAGCC GCCCTCACAG 
TCAGGCCGTT TTTCCGTGTC TCTGAGCGGA GAGCTTACCA TCACAGATGT GCACAGCGAG 
GATTCTGGCT ACTACATCTG CCAGGCTATC AGTGTGGCGG GCAGCATCTT GACCAAAGCA 
CTGCTGGAGG TGGAAAGCAC TCCATCTGAC CGGATCCCTC CCATCATCCG CCAAGGCCCG 
GCCAACCAGA CGCTAGCTCC GGGCACCACG GCCCAATTGC AGTGCCACGT GATGGGCAAC 
CCTCTTCCGA GCATCCAATG GGAGCGCGAC GGACAACGAA TCCTGGGCAT CGACGAGCGC 
ATTAGCCTAA TGGAAAACGG CACTTTGCAA ATCACAGCCC TGCAGGAGAC GGACTCCGGG 
GCCTACACCT GTGTTGCTTC CAGTCTGAGT GGAGAGACGA GCTGGAGTGG TGTGCTCACA 
GTAAAAGAGA GTGGTGGTCT ATCAGCATCT CCGGTATCAG AGCCCTATCA ACTGCCCGGT 
CCTCCTCAGA AACCTGTCGT AACGGATGTA ACCCGTAACA GTGTCACCCT CACCTGGCAA 
CCCAACGCAC ACGAAGGCGG CGCCGCCGTC ACCTCTTACA TCATCGAGGC CTTCAGTCAA 
TCGGCAGGCA GTACGTGGCA GACGGTTGCA GATTTTGTGA AGCTGGAAAA GCACACGGTC 
ACCGGATTGT CTCCGAACAC CATTTACCTG TTTATTGTGC GAGCCGTCAA TGCATATGGC 
CTGAGTGACC CAAGCCCAAT TTCAGAGCCC GTACGCACAC AAGATGTGAG TCCCACAGGT 
CAGGGTGTTG ACCATCGTCA GGTGCAGAGG GAGCTGGGAG AAATGTCTGT ACAGCTTCAC 
GAACCCGTGC TGTTGACGGC CTCCAGTGTG AAGATCAGCT GGAACGTGGA CCGTCAGTCG 
CAGTACATCC AGGGTTATCG GCTGCTTTAT CGGCCTGTCG GCGGTTCCTG GTCCCAGCAG 
GAAGTGAAGG GTGCAACAGA GCGTAGCGCC GTCATCGCTA ACCTGCTCAA AGGCACCGAG 
TACGAGATTA AAATACGACC CTACTTTAAC GAGTTTCAGG GCCTGGACAG CCGATCGCAG 
ACACTCCGCA CGCCAGAGGA GGTGCCGAGT GCTCCTCCTC GAGCTGTGAA TGTGGCCACA 
GTGAAGCTCA GTAACAGCTC TAGCATCAGT GTTTCATGGC AGCCTCCACC CACAGACATG 
CAGAATGGAG TCATTCAGGA GTACAAGGTT TGGTGTCTGG GCAATGATTC TCAGACACGC 
TACAACATCA ATCAGACGGT GGACGGCAGT GTGTTAAGCA CTGTACTGAA GGGTCTGCTG 
CCTGGCGTCC TGTACCAGGT GGAGGTGGCA GCAGTGACCA GTGCAGGAGT GGGCAAACAC 
AGCCAACCCG TATCTGTTCT TATCAAATTG CCGGCAGAGC AGCCCACAGT CACAGGTGGA 
GGCAGTGAAT CTGAGGAGAA TGTAAGTCTG GCGGAGCAGA TCACAGACGT GGTGAAGCAG 
CCTGCCTTCA TCGCAGGCAT TGGCGGAGCA TGCTGGGTAA TCCTGATGGG ATTCAGCGTG 
TGGATCTACT GCCGACGCAA GAAGAGAAAA GAACTCAGCC ATTACACTGC GTCATTTGCA 
TACACACCGG CAGTTGGTTT TCCACATGGT GAAAGTTCTG GACTTAACGG AAGGCCTGGT 
ATGCTGGGTG CTAACATGGG AAATTATCCA TGGCTGGCTG ATTCTTGGCC TACTACCAAT 
CTTGTGCACA ACGGGAAAGA CTCAGTGAAC TGCTGTACAT CTAAACACGA AACACCGGAG 
AGATACTATA ACGAGGCGGG AATCTCTAAT TACCTCAATC AAAATGAGAA GTACAGCGCA 
GACTCCAACG AGGGGCCGAT CTACAGCACC ATCGACCCCA CCAGCGAAGA CTTACGCAGT 
TTTACCGCTC AGTATTCTCA GCACACCACA CCCTACGCCA GCACTCCCAT TATGCCTTTC 
ACCAACCAGG CGCCCTGCAG CCCTGAGCAA GATGGCAGCC ACTGGATGAC GCAGCCGGGA 
ACCTCCAATG CTCAGTATGC CCAGCCAGAC CGATCCAAGA CTGAGAACGT AAAACAACCA 
AAGCAGAAGT TGATGGGGAA GCCGGTGAAA ACCCCATCTC TCAACTGGAT GGATGCGCTG 
CCGCCTCCAC CCATCTCTGG AGATCTGGAG CAGTGTGACG AGGAGGATGA TAACGAACTA 
ATACAAGACA TGGGGTCTGA TGAGGAAGAG TGGTGTCCTC CTCTTCCCGA GCGGTCTTAT 
CTCATGGACG GCATGGTGGA GGACGGTCCA GTTCCACCAC CAAGAGGAGA AGCTTCTTCA 
CCCACTACGT CCTACAGCCA CCAGTCCACC GCCACCTTAA CCCCTTCTCC CCGAGACGAT 
GGACACTTCC ACGATATACC ACGCTTACAC CAGCTCGAAC AACCACACAT GACACGCCGG 
TTGCCGTGTG GACCAGTGCC TGTGCCACGA GCACCAAGCC CACCTCTAAC CCAATCCAAC 
CCAAACCTGT CAGCCAATCA GGAGTGTCCT GACAACACTG TGCCACACCG TTCAGCTCAT 
AAACGAGACC TGAGCCAAGG AGCTGCACAA AGTGTAGAGA ACATCAGCAA CACCACACCA 
GGTCGAGCGA GGCCCTCCGC AGACCACACA TCGTCTGGGC AGAAATCCAA GGTGAAGAAG 
AAAGCTTCCA AGAGCGCTCA ATACCGGAGA GATATACTGC AGGGCGATCT GCCTCCTCCA 
CCAGAGCCTC CACCCGAAGA CCAGGATAAT TGCGCTCATG TAGGGGGCTT CGAATGCGGG 
CAGACGTCCC TCGAGAGAGC CGGCAGCTCC AATTCGTCCA TGGAGAGGAG AGAGCAGAGC 
ACACCCAACC GGAAAAACTC GGCCCATAGA CGGATAGACG CAGATGACGA TTTAATTCCT 
TACAACAAGC CTAACTTCCT GTCCCGAGGT CAGATGTCCA GCAATTGCTC CACAACAGGA 
AGTTCATCAT CACGAGGGTC AACGGGGTCG CGAGGGCAAG GAACGGGCAG GAAGAGAAAT 
GATGAGATGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172693.1
CDS	439..4713
Translation	MLRYLIKTLLQMNLFTDTIRDATNGTDLFFNISSIPSFNSSLLDWANFTGLNGSRPRLEDAAPRIVEHPSDLIVSKGEPATLNCKAEGRPTPMVEWYKDGERVETDREDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYVCVARNYLGEAVSRNASLEVAILRDDFRQTPSDVVVAAGEPAVMECIPPRGHPEPTISWKRNNVKVNDKDERITIRGGKLMISNTRKSDAGMYVCVGTNMVGEKDSDPAELVVFERPMFVRRPVNQVVLADDTVDFQCEVQGDPAPTIRWKKEEGELPRGRFEIRADNSLRLTQVKAEDEGSYTCLSENSVGKAEASGSLQVHVGPSLPPQIVVRPRDQISAQGRTVTFLCGTKGNPPPAVFWQKEGSQVLLFPSQPPSQSGRFSVSLSGELTITDVHSEDSGYYICQAISVAGSILTKALLEVESTPSDRIPPIIRQGPANQTLAPGTTAQLQCHVMGNPLPSIQWERDGQRILGIDERISLMENGTLQITALQETDSGAYTCVASSLSGETSWSGVLTVKESGGLSASPVSEPYQLPGPPQKPVVTDVTRNSVTLTWQPNAHEGGAAVTSYIIEAFSQSAGSTWQTVADFVKLEKHTVTGLSPNTIYLFIVRAVNAYGLSDPSPISEPVRTQDVSPTGQGVDHRQVQRELGEMSVQLHEPVLLTASSVKISWNVDRQSQYIQGYRLLYRPVGGSWSQQEVKGATERSAVIANLLKGTEYEIKIRPYFNEFQGLDSRSQTLRTPEEVPSAPPRAVNVATVKLSNSSSISVSWQPPPTDMQNGVIQEYKVWCLGNDSQTRYNINQTVDGSVLSTVLKGLLPGVLYQVEVAAVTSAGVGKHSQPVSVLIKLPAEQPTVTGGGSESEENVSLAEQITDVVKQPAFIAGIGGACWVILMGFSVWIYCRRKKRKELSHYTASFAYTPAVGFPHGESSGLNGRPGMLGANMGNYPWLADSWPTTNLVHNGKDSVNCCTSKHETPERYYNEAGISNYLNQNEKYSADSNEGPIYSTIDPTSEDLRSFTAQYSQHTTPYASTPIMPFTNQAPCSPEQDGSHWMTQPGTSNAQYAQPDRSKTENVKQPKQKLMGKPVKTPSLNWMDALPPPPISGDLEQCDEEDDNELIQDMGSDEEEWCPPLPERSYLMDGMVEDGPVPPPRGEASSPTTSYSHQSTATLTPSPRDDGHFHDIPRLHQLEQPHMTRRLPCGPVPVPRAPSPPLTQSNPNLSANQECPDNTVPHRSAHKRDLSQGAAQSVENISNTTPGRARPSADHTSSGQKSKVKKKASKSAQYRRDILQGDLPPPPEPPPEDQDNCAHVGGFECGQTSLERAGSSNSSMERREQSTPNRKNSAHRRIDADDDLIPYNKPNFLSRGQMSSNCSTTGSSSSRGSTGSRGQGTGRKRNDEMR

Target ORF information:

RefSeq Version	XM_005172636.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172636.3

ORF Insert Sequence:

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TGGGCCCATC TCTTCCTCCT CAGATTGTAG TGCGTCCGCG GGATCAGATC 
TCTGCCCAGG GCCGCACTGT CACCTTTCTC TGCGGAACGA AGGGAAATCC CCCTCCTGCT 
GTTTTCTGGC AGAAAGAAGG CAGTCAGGTC CTGCTGTTTC CCAGTCAGCC GCCCTCACAG 
TCAGGCCGTT TTTCCGTGTC TCTGAGCGGA GAGCTTACCA TCACAGATGT GCACAGCGAG 
GATTCTGGCT ACTACATCTG CCAGGCTATC AGTGTGGCGG GCAGCATCTT GACCAAAGCA 
CTGCTGGAGG TGGAAAGCAC TCCATCTGAC CGGATCCCTC CCATCATCCG CCAAGGCCCG 
GCCAACCAGA CGCTAGCTCC GGGCACCACG GCCCAATTGC AGTGCCACGT GATGGGCAAC 
CCTCTTCCGA GCATCCAATG GGAGCGCGAC GGACAACGAA TCCTGGGCAT CGACGAGCGC 
ATTAGCCTAA TGGAAAACGG CACTTTGCAA ATCACAGCCC TGCAGGAGAC GGACTCCGGG 
GCCTACACCT GTGTTGCTTC CAGTCTGAGT GGAGAGACGA GCTGGAGTGG TGTGCTCACA 
GTAAAAGAGA GTGGTGGTCT ATCAGCATCT CCGGTATCAG AGCCCTATCA ACTGCCCGGT 
CCTCCTCAGA AACCTGTCGT AACGGATGTA ACCCGTAACA GTGTCACCCT CACCTGGCAA 
CCCAACGCAC ACGAAGGCGG CGCCGCCGTC ACCTCTTACA TCATCGAGGC CTTCAGTCAA 
TCGGCAGGCA GTACGTGGCA GACGGTTGCA GATTTTGTGA AGCTGGAAAA GCACACGGTC 
ACCGGATTGT CTCCGAACAC CATTTACCTG TTTATTGTGC GAGCCGTCAA TGCATATGGC 
CTGAGTGACC CAAGCCCAAT TTCAGAGCCC GTACGCACAC AAGATGTGAG TCCCACAGGT 
CAGGGTGTTG ACCATCGTCA GGTGCAGAGG GAGCTGGGAG AAATGTCTGT ACAGCTTCAC 
GAACCCGTGC TGTTGACGGC CTCCAGTGTG AAGATCAGCT GGAACGTGGA CCGTCAGTCG 
CAGTACATCC AGGGTTATCG GCTGCTTTAT CGGCCTGTCG GCGGTTCCTG GTCCCAGCAG 
GAAGTGAAGG GTGCAACAGA GCGTAGCGCC GTCATCGCTA ACCTGCTCAA AGGCACCGAG 
TACGAGATTA AAATACGACC CTACTTTAAC GAGTTTCAGG GCCTGGACAG CCGATCGCAG 
ACACTCCGCA CGCCAGAGGA GGTGCCGAGT GCTCCTCCTC GAGCTGTGAA TGTGGCCACA 
GTGAAGCTCA GTAACAGCTC TAGCATCAGT GTTTCATGGC AGCCTCCACC CACAGACATG 
CAGAATGGAG TCATTCAGGA GTACAAGGTT TGGTGTCTGG GCAATGATTC TCAGACACGC 
TACAACATCA ATCAGACGGT GGACGGCAGT GTGTTAAGCA CTGTACTGAA GGGTCTGCTG 
CCTGGCGTCC TGTACCAGGT GGAGGTGGCA GCAGTGACCA GTGCAGGAGT GGGCAAACAC 
AGCCAACCCG TATCTGTTCT TATCAAATTG CCGGCAGAGC AGCCCACAGT CACAGGTGGA 
GGCAGTGAAT CTGAGGAGAA TGTAAGTCTG GCGGAGCAGA TCACAGACGT GGTGAAGCAG 
CCTGCCTTCA TCGCAGGCAT TGGCGGAGCA TGCTGGGTAA TCCTGATGGG ATTCAGCGTG 
TGGATCTACT GCCGACGCAA GAAGAGAAAA GAACTCAGCC ATTACACTGC GTCATTTGCA 
TACACACCGG CAGTTGGTTT TCCACATGGT GAAAGTTCTG GACTTAACGG AAGGCCTGGT 
ATGCTGGGTG CTAACATGGG AAATTATCCA TGGCTGGCTG ATTCTTGGCC TACTACCAAT 
CTTGTGCACA ACGGGAAAGA CTCAGTGAAC TGCTGTACAT CTAAACACGA AACACCGGAG 
AGATACTATA ACGAGGCGGG AATCTCTAAT TACCTCAATC AAAATGAGAA GTACAGCGCA 
GACTCCAACG AGGGGCCGAT CTACAGCACC ATCGACCCCA CCAGCGAAGA CTTACGCAGT 
TTTACCGCTC AGTATTCTCA GCACACCACA CCCTACGCCA GCACTCCCAT TATGCCTTTC 
ACCAACCAGG CGCCCTGCAG CCCTGAGCAA GATGGCAGCC ACTGGATGAC GCAGCCGGGA 
ACCTCCAATG CTCAGTATGC CCAGCCAGAC CGATCCAAGA CTGAGAACGT AAAACAACCA 
AAGCAGAAGT TGATGGGGAA GCCGGTGAAA ACCCCATCTC TCAACTGGAT GGATGCGCTG 
CCGCCTCCAC CCATCTCTGG AGATCTGGAG CAGTGTGACG AGGAGGATGA TAACGAACTA 
ATACAAGACA TGGGGTCTGA TGAGGAAGAG TGGTGTCCTC CTCTTCCCGA GCGGTCTTAT 
CTCATGGACG GCATGGTGGA GGACGGTCCA GTTCCACCAC CAAGAGGAGA AGCTTCTTCA 
CCCACTACGT CCTACAGCCA CCAGTCCACC GCCACCTTAA CCCCTTCTCC CCGAGACGAT 
GGACACTTCC ACGATATACC ACGCTTACAC CAGCTCGAAC AACCACACAT GACACGCCGG 
TTGCCGTGTG GACCAGTGCC TGTGCCACGA GCACCAAGCC CACCTCTAAC CCAATCCAAC 
CCAAACCTGT CAGCCAATCA GGAGTGTCCT GACAACACTG TGCCACACCG TTCAGCTCAT 
AAACGAGACC TGAGCCAAGG AGCTGCACAA AGTGTAGAGA ACATCAGCAA CACCACACCA 
GGTCGAGCGA GGCCCTCCGC AGACCACACA TCGTCTGGGC AGAAATCCAA GGTGAAGAAG 
AAAGCTTCCA AGAGCGCTCA ATACCGGAGA GATATACTGC AGGGCGATCT GCCTCCTCCA 
CCAGAGCCTC CACCCGAAGA CCAGGATAAT TGCGCTCATG TAGGGGGCTT CGAATGCGGG 
CAGACGTCCC TCGAGAGAGC CGGCAGCTCC AATTCGTCCA TGGAGAGGAG AGAGCAGAGC 
ACACCCAACC GGAAAAACTC GGCCCATAGA CGGATAGACG CAGATGACGA TTTAATTCCT 
TACAACAAGC CTAACTTCCT GTCCCGAGGT CAGATGTCCA GCAATTGCTC CACAACAGGA 
AGTTCATCAT CACGAGGGTC AACGGGGTCG CGAGGGCAAG GAACGGGCAG GAAGAGAAAT 
GATGAGATGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_005172637.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172694.1 Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X2, mRNA.

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CloneID	ODa16200
Clone ID Related Accession (Same CDS sequence)	XM_005172637.3 , XM_005172637.4
Accession Version	XM_005172637.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172637.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TGGGCCCATC TCTTCCTCCT CAGATTGTAG TGCGTCCGCG GGATCAGATC 
TCTGCCCAGG GCCGCACTGT CACCTTTCTC TGCGGAACGA AGGGAAATCC CCCTCCTGCT 
GTTTTCTGGC AGAAAGAAGG CAGTCAGGTC CTGCTGTTTC CCAGTCAGCC GCCCTCACAG 
TCAGGCCGTT TTTCCGTGTC TCTGAGCGGA GAGCTTACCA TCACAGATGT GCACAGCGAG 
GATTCTGGCT ACTACATCTG CCAGGCTATC AGTGTGGCGG GCAGCATCTT GACCAAAGCA 
CTGCTGGAGG TGGAAAGCAC TCCATCTGAC CGGATCCCTC CCATCATCCG CCAAGGCCCG 
GCCAACCAGA CGCTAGCTCC GGGCACCACG GCCCAATTGC AGTGCCACGT GATGGGCAAC 
CCTCTTCCGA GCATCCAATG GGAGCGCGAC GGACAACGAA TCCTGGGCAT CGACGAGCGC 
ATTAGCCTAA TGGAAAACGG CACTTTGCAA ATCACAGCCC TGCAGGAGAC GGACTCCGGG 
GCCTACACCT GTGTTGCTTC CAGTCTGAGT GGAGAGACGA GCTGGAGTGG TGTGCTCACA 
GTAAAAGAGA GTGGTGGTCT ATCAGCATCT CCGGTATCAG AGCCCTATCA ACTGCCCGGT 
CCTCCTCAGA AACCTGTCGT AACGGATGTA ACCCGTAACA GTGTCACCCT CACCTGGCAA 
CCCAACGCAC ACGAAGGCGG CGCCGCCGTC ACCTCTTACA TCATCGAGGC CTTCAGTCAA 
TCGGCAGGCA GTACGTGGCA GACGGTTGCA GATTTTGTGA AGCTGGAAAA GCACACGGTC 
ACCGGATTGT CTCCGAACAC CATTTACCTG TTTATTGTGC GAGCCGTCAA TGCATATGGC 
CTGAGTGACC CAAGCCCAAT TTCAGAGCCC GTACGCACAC AAGATGTGAG TCCCACAGGT 
CAGGGTGTTG ACCATCGTCA GGTGCAGAGG GAGCTGGGAG AAATGTCTGT ACAGCTTCAC 
GAACCCGTGC TGTTGACGGC CTCCAGTGTG AAGATCAGCT GGAACGTGGA CCGTCAGTCG 
CAGTACATCC AGGGTTATCG GCTGCTTTAT CGGCCTGTCG GCGGTTCCTG GTCCCAGCAG 
GAAGTGAAGG GTGCAACAGA GCGTAGCGCC GTCATCGCTA ACCTGCTCAA AGGCACCGAG 
TACGAGATTA AAATACGACC CTACTTTAAC GAGTTTCAGG GCCTGGACAG CCGATCGCAG 
ACACTCCGCA CGCCAGAGGA GGTGCCGAGT GCTCCTCCTC GAGCTGTGAA TGTGGCCACA 
GTGAAGCTCA GTAACAGCTC TAGCATCAGT GTTTCATGGC AGCCTCCACC CACAGACATG 
CAGAATGGAG TCATTCAGGA GTACAAGGTT TGGTGTCTGG GCAATGATTC TCAGACACGC 
TACAACATCA ATCAGACGGT GGACGGCAGT GTGTTAAGCA CTGTACTGAA GGGTCTGCTG 
CCTGGCGTCC TGTACCAGGT GGAGGTGGCA GCAGTGACCA GTGCAGGAGT GGGCAAACAC 
AGCCAACCCG TATCTGTTCT TATCAAATTG CCGGCAGAGC AGCCCACAGT CACAGGTGGA 
GGCAGTGAAT CTGAGGAGAA TGTAAGTCTG GCGGAGCAGA TCACAGACGT GGTGAAGCAG 
CCTGCCTTCA TCGCAGGCAT TGGCGGAGCA TGCTGGGTAA TCCTGATGGG ATTCAGCGTG 
TGGATCTACT GCCGACGCAA GAAGAGAAAA GAACTCAGCC ATTACACTGC GTCATTTGCA 
TACACACCGG CAGTTGGTTT TCCACATGGT GAAAGTTCTG GACTTAACGG AAGGCCTGGT 
ATGCTGGGTG CTAACATGGG AAATTATCCA TGGCTGGCTG ATTCTTGGCC TACTACCAAT 
CTTGTGCACA ACGGGAAAGA CTCAGTGAAC TGCTGTACAT CTAAACACGA AACACCGGAG 
AGATACTATA ACGAGGCGGG AATCTCTAAT TACCTCAATC AAAATGAGAA GTACAGCGCA 
GACTCCAACG AGGGGCCGAT CTACAGCACC ATCGACCCCA CCAGCGAAGA CTTACGCAGT 
TTTACCGCTC AGTATTCTCA GCACACCACA CCCTACGCCA GCACTCCCAT TATGCCTTTC 
ACCAACCAGG CGCCCTGCAG CCCTGAGCAA GATGGCAGCC ACTGGATGAC GCAGCCGGGA 
ACCTCCAATG CTCAGTATGC CCAGCCAGAC CGATCCAAGA CTGAGAACGT AAAACAACCA 
AAGCAGAAGT TGATGGGGAA GCCGGTGAAA ACCCCATCTC TCAACTGGAT GGATGCGCTG 
CCGCCTCCAC CCATCTCTGG AGATCTGGAG CAGTGTGACG AGGAGGATGA TAACGAACTA 
ATACAAGACA TGGGGTCTGA TGAGGAAGAG TGGTGTCCTC CTCTTCCCGA GCGGTCTTAT 
CTCATGGACG GCATGGTGGA GGACGGTCCA GTTCCACCAC CAAGAGGAGA AGCTTCTTCA 
CCCACTACGT CCTACAGCCA CCAGTCCACC GCCACCTTAA CCCCTTCTCC CCGAGACGAT 
GGACACTTCC ACGATATACC ACGCTTACAC CAGCTCGAAC AACCACACAT GACACGCCGG 
TTGCCGTGTG GACCAGTGCC TGTGCCACGA GCACCAAGCC CACCTCTAAC CCAATCCAAC 
CCAAACCTGT CAGCCAATCA GGAGTGTCCT GACAACACTG TGCCACACCG TTCAGCTCAT 
AAACGAGACC TGAGCCAAGG AGCTGCACAA AGTGTAGAGA ACATCAGCAA CACCACACCA 
GGTCGAGCGA GGCCCTCCGC AGACCACACA TCGTCTGGGC AGAAATCCAA GGTGAAGAAG 
AAAGCTTCCA AGAGCGCTCA ATACCGGAGA GATATACTGC AGGGCGATCT GCCTCCTCCA 
CCAGAGCCTC CACCCGAAGA CCAGGATAAT TGCGCTCATG TAGGGGGCTT CGAATGCGGG 
CAGACGTCCC TCGAGAGAGC CGGCAGCTCC AATTCGTCCA TGGAGAGGAG AGAGCAGAGC 
ACACCCAACC GGAAAAACTC GGCCCATAGA CGGATAGACG ATGACGATTT AATTCCTTAC 
AACAAGCCTA ACTTCCTGTC CCGAGGTCAG ATGTCCAGCA ATTGCTCCAC AACAGGAAGT 
TCATCATCAC GAGGGTCAAC GGGGTCGCGA GGGCAAGGAA CGGGCAGGAA GAGAAATGAT 
GAGATGAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172694.1
CDS	439..4710
Translation	MLRYLIKTLLQMNLFTDTIRDATNGTDLFFNISSIPSFNSSLLDWANFTGLNGSRPRLEDAAPRIVEHPSDLIVSKGEPATLNCKAEGRPTPMVEWYKDGERVETDREDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYVCVARNYLGEAVSRNASLEVAILRDDFRQTPSDVVVAAGEPAVMECIPPRGHPEPTISWKRNNVKVNDKDERITIRGGKLMISNTRKSDAGMYVCVGTNMVGEKDSDPAELVVFERPMFVRRPVNQVVLADDTVDFQCEVQGDPAPTIRWKKEEGELPRGRFEIRADNSLRLTQVKAEDEGSYTCLSENSVGKAEASGSLQVHVGPSLPPQIVVRPRDQISAQGRTVTFLCGTKGNPPPAVFWQKEGSQVLLFPSQPPSQSGRFSVSLSGELTITDVHSEDSGYYICQAISVAGSILTKALLEVESTPSDRIPPIIRQGPANQTLAPGTTAQLQCHVMGNPLPSIQWERDGQRILGIDERISLMENGTLQITALQETDSGAYTCVASSLSGETSWSGVLTVKESGGLSASPVSEPYQLPGPPQKPVVTDVTRNSVTLTWQPNAHEGGAAVTSYIIEAFSQSAGSTWQTVADFVKLEKHTVTGLSPNTIYLFIVRAVNAYGLSDPSPISEPVRTQDVSPTGQGVDHRQVQRELGEMSVQLHEPVLLTASSVKISWNVDRQSQYIQGYRLLYRPVGGSWSQQEVKGATERSAVIANLLKGTEYEIKIRPYFNEFQGLDSRSQTLRTPEEVPSAPPRAVNVATVKLSNSSSISVSWQPPPTDMQNGVIQEYKVWCLGNDSQTRYNINQTVDGSVLSTVLKGLLPGVLYQVEVAAVTSAGVGKHSQPVSVLIKLPAEQPTVTGGGSESEENVSLAEQITDVVKQPAFIAGIGGACWVILMGFSVWIYCRRKKRKELSHYTASFAYTPAVGFPHGESSGLNGRPGMLGANMGNYPWLADSWPTTNLVHNGKDSVNCCTSKHETPERYYNEAGISNYLNQNEKYSADSNEGPIYSTIDPTSEDLRSFTAQYSQHTTPYASTPIMPFTNQAPCSPEQDGSHWMTQPGTSNAQYAQPDRSKTENVKQPKQKLMGKPVKTPSLNWMDALPPPPISGDLEQCDEEDDNELIQDMGSDEEEWCPPLPERSYLMDGMVEDGPVPPPRGEASSPTTSYSHQSTATLTPSPRDDGHFHDIPRLHQLEQPHMTRRLPCGPVPVPRAPSPPLTQSNPNLSANQECPDNTVPHRSAHKRDLSQGAAQSVENISNTTPGRARPSADHTSSGQKSKVKKKASKSAQYRRDILQGDLPPPPEPPPEDQDNCAHVGGFECGQTSLERAGSSNSSMERREQSTPNRKNSAHRRIDDDDLIPYNKPNFLSRGQMSSNCSTTGSSSSRGSTGSRGQGTGRKRNDEMR

Target ORF information:

RefSeq Version	XM_005172637.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172637.3

ORF Insert Sequence:

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TGGGCCCATC TCTTCCTCCT CAGATTGTAG TGCGTCCGCG GGATCAGATC 
TCTGCCCAGG GCCGCACTGT CACCTTTCTC TGCGGAACGA AGGGAAATCC CCCTCCTGCT 
GTTTTCTGGC AGAAAGAAGG CAGTCAGGTC CTGCTGTTTC CCAGTCAGCC GCCCTCACAG 
TCAGGCCGTT TTTCCGTGTC TCTGAGCGGA GAGCTTACCA TCACAGATGT GCACAGCGAG 
GATTCTGGCT ACTACATCTG CCAGGCTATC AGTGTGGCGG GCAGCATCTT GACCAAAGCA 
CTGCTGGAGG TGGAAAGCAC TCCATCTGAC CGGATCCCTC CCATCATCCG CCAAGGCCCG 
GCCAACCAGA CGCTAGCTCC GGGCACCACG GCCCAATTGC AGTGCCACGT GATGGGCAAC 
CCTCTTCCGA GCATCCAATG GGAGCGCGAC GGACAACGAA TCCTGGGCAT CGACGAGCGC 
ATTAGCCTAA TGGAAAACGG CACTTTGCAA ATCACAGCCC TGCAGGAGAC GGACTCCGGG 
GCCTACACCT GTGTTGCTTC CAGTCTGAGT GGAGAGACGA GCTGGAGTGG TGTGCTCACA 
GTAAAAGAGA GTGGTGGTCT ATCAGCATCT CCGGTATCAG AGCCCTATCA ACTGCCCGGT 
CCTCCTCAGA AACCTGTCGT AACGGATGTA ACCCGTAACA GTGTCACCCT CACCTGGCAA 
CCCAACGCAC ACGAAGGCGG CGCCGCCGTC ACCTCTTACA TCATCGAGGC CTTCAGTCAA 
TCGGCAGGCA GTACGTGGCA GACGGTTGCA GATTTTGTGA AGCTGGAAAA GCACACGGTC 
ACCGGATTGT CTCCGAACAC CATTTACCTG TTTATTGTGC GAGCCGTCAA TGCATATGGC 
CTGAGTGACC CAAGCCCAAT TTCAGAGCCC GTACGCACAC AAGATGTGAG TCCCACAGGT 
CAGGGTGTTG ACCATCGTCA GGTGCAGAGG GAGCTGGGAG AAATGTCTGT ACAGCTTCAC 
GAACCCGTGC TGTTGACGGC CTCCAGTGTG AAGATCAGCT GGAACGTGGA CCGTCAGTCG 
CAGTACATCC AGGGTTATCG GCTGCTTTAT CGGCCTGTCG GCGGTTCCTG GTCCCAGCAG 
GAAGTGAAGG GTGCAACAGA GCGTAGCGCC GTCATCGCTA ACCTGCTCAA AGGCACCGAG 
TACGAGATTA AAATACGACC CTACTTTAAC GAGTTTCAGG GCCTGGACAG CCGATCGCAG 
ACACTCCGCA CGCCAGAGGA GGTGCCGAGT GCTCCTCCTC GAGCTGTGAA TGTGGCCACA 
GTGAAGCTCA GTAACAGCTC TAGCATCAGT GTTTCATGGC AGCCTCCACC CACAGACATG 
CAGAATGGAG TCATTCAGGA GTACAAGGTT TGGTGTCTGG GCAATGATTC TCAGACACGC 
TACAACATCA ATCAGACGGT GGACGGCAGT GTGTTAAGCA CTGTACTGAA GGGTCTGCTG 
CCTGGCGTCC TGTACCAGGT GGAGGTGGCA GCAGTGACCA GTGCAGGAGT GGGCAAACAC 
AGCCAACCCG TATCTGTTCT TATCAAATTG CCGGCAGAGC AGCCCACAGT CACAGGTGGA 
GGCAGTGAAT CTGAGGAGAA TGTAAGTCTG GCGGAGCAGA TCACAGACGT GGTGAAGCAG 
CCTGCCTTCA TCGCAGGCAT TGGCGGAGCA TGCTGGGTAA TCCTGATGGG ATTCAGCGTG 
TGGATCTACT GCCGACGCAA GAAGAGAAAA GAACTCAGCC ATTACACTGC GTCATTTGCA 
TACACACCGG CAGTTGGTTT TCCACATGGT GAAAGTTCTG GACTTAACGG AAGGCCTGGT 
ATGCTGGGTG CTAACATGGG AAATTATCCA TGGCTGGCTG ATTCTTGGCC TACTACCAAT 
CTTGTGCACA ACGGGAAAGA CTCAGTGAAC TGCTGTACAT CTAAACACGA AACACCGGAG 
AGATACTATA ACGAGGCGGG AATCTCTAAT TACCTCAATC AAAATGAGAA GTACAGCGCA 
GACTCCAACG AGGGGCCGAT CTACAGCACC ATCGACCCCA CCAGCGAAGA CTTACGCAGT 
TTTACCGCTC AGTATTCTCA GCACACCACA CCCTACGCCA GCACTCCCAT TATGCCTTTC 
ACCAACCAGG CGCCCTGCAG CCCTGAGCAA GATGGCAGCC ACTGGATGAC GCAGCCGGGA 
ACCTCCAATG CTCAGTATGC CCAGCCAGAC CGATCCAAGA CTGAGAACGT AAAACAACCA 
AAGCAGAAGT TGATGGGGAA GCCGGTGAAA ACCCCATCTC TCAACTGGAT GGATGCGCTG 
CCGCCTCCAC CCATCTCTGG AGATCTGGAG CAGTGTGACG AGGAGGATGA TAACGAACTA 
ATACAAGACA TGGGGTCTGA TGAGGAAGAG TGGTGTCCTC CTCTTCCCGA GCGGTCTTAT 
CTCATGGACG GCATGGTGGA GGACGGTCCA GTTCCACCAC CAAGAGGAGA AGCTTCTTCA 
CCCACTACGT CCTACAGCCA CCAGTCCACC GCCACCTTAA CCCCTTCTCC CCGAGACGAT 
GGACACTTCC ACGATATACC ACGCTTACAC CAGCTCGAAC AACCACACAT GACACGCCGG 
TTGCCGTGTG GACCAGTGCC TGTGCCACGA GCACCAAGCC CACCTCTAAC CCAATCCAAC 
CCAAACCTGT CAGCCAATCA GGAGTGTCCT GACAACACTG TGCCACACCG TTCAGCTCAT 
AAACGAGACC TGAGCCAAGG AGCTGCACAA AGTGTAGAGA ACATCAGCAA CACCACACCA 
GGTCGAGCGA GGCCCTCCGC AGACCACACA TCGTCTGGGC AGAAATCCAA GGTGAAGAAG 
AAAGCTTCCA AGAGCGCTCA ATACCGGAGA GATATACTGC AGGGCGATCT GCCTCCTCCA 
CCAGAGCCTC CACCCGAAGA CCAGGATAAT TGCGCTCATG TAGGGGGCTT CGAATGCGGG 
CAGACGTCCC TCGAGAGAGC CGGCAGCTCC AATTCGTCCA TGGAGAGGAG AGAGCAGAGC 
ACACCCAACC GGAAAAACTC GGCCCATAGA CGGATAGACG ATGACGATTT AATTCCTTAC 
AACAAGCCTA ACTTCCTGTC CCGAGGTCAG ATGTCCAGCA ATTGCTCCAC AACAGGAAGT 
TCATCATCAC GAGGGTCAAC GGGGTCGCGA GGGCAAGGAA CGGGCAGGAA GAGAAATGAT 
GAGATGAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_005172637.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172694.1 Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa16200
Clone ID Related Accession (Same CDS sequence)	XM_005172637.3 , XM_005172637.4
Accession Version	XM_005172637.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172637.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TGGGCCCATC TCTTCCTCCT CAGATTGTAG TGCGTCCGCG GGATCAGATC 
TCTGCCCAGG GCCGCACTGT CACCTTTCTC TGCGGAACGA AGGGAAATCC CCCTCCTGCT 
GTTTTCTGGC AGAAAGAAGG CAGTCAGGTC CTGCTGTTTC CCAGTCAGCC GCCCTCACAG 
TCAGGCCGTT TTTCCGTGTC TCTGAGCGGA GAGCTTACCA TCACAGATGT GCACAGCGAG 
GATTCTGGCT ACTACATCTG CCAGGCTATC AGTGTGGCGG GCAGCATCTT GACCAAAGCA 
CTGCTGGAGG TGGAAAGCAC TCCATCTGAC CGGATCCCTC CCATCATCCG CCAAGGCCCG 
GCCAACCAGA CGCTAGCTCC GGGCACCACG GCCCAATTGC AGTGCCACGT GATGGGCAAC 
CCTCTTCCGA GCATCCAATG GGAGCGCGAC GGACAACGAA TCCTGGGCAT CGACGAGCGC 
ATTAGCCTAA TGGAAAACGG CACTTTGCAA ATCACAGCCC TGCAGGAGAC GGACTCCGGG 
GCCTACACCT GTGTTGCTTC CAGTCTGAGT GGAGAGACGA GCTGGAGTGG TGTGCTCACA 
GTAAAAGAGA GTGGTGGTCT ATCAGCATCT CCGGTATCAG AGCCCTATCA ACTGCCCGGT 
CCTCCTCAGA AACCTGTCGT AACGGATGTA ACCCGTAACA GTGTCACCCT CACCTGGCAA 
CCCAACGCAC ACGAAGGCGG CGCCGCCGTC ACCTCTTACA TCATCGAGGC CTTCAGTCAA 
TCGGCAGGCA GTACGTGGCA GACGGTTGCA GATTTTGTGA AGCTGGAAAA GCACACGGTC 
ACCGGATTGT CTCCGAACAC CATTTACCTG TTTATTGTGC GAGCCGTCAA TGCATATGGC 
CTGAGTGACC CAAGCCCAAT TTCAGAGCCC GTACGCACAC AAGATGTGAG TCCCACAGGT 
CAGGGTGTTG ACCATCGTCA GGTGCAGAGG GAGCTGGGAG AAATGTCTGT ACAGCTTCAC 
GAACCCGTGC TGTTGACGGC CTCCAGTGTG AAGATCAGCT GGAACGTGGA CCGTCAGTCG 
CAGTACATCC AGGGTTATCG GCTGCTTTAT CGGCCTGTCG GCGGTTCCTG GTCCCAGCAG 
GAAGTGAAGG GTGCAACAGA GCGTAGCGCC GTCATCGCTA ACCTGCTCAA AGGCACCGAG 
TACGAGATTA AAATACGACC CTACTTTAAC GAGTTTCAGG GCCTGGACAG CCGATCGCAG 
ACACTCCGCA CGCCAGAGGA GGTGCCGAGT GCTCCTCCTC GAGCTGTGAA TGTGGCCACA 
GTGAAGCTCA GTAACAGCTC TAGCATCAGT GTTTCATGGC AGCCTCCACC CACAGACATG 
CAGAATGGAG TCATTCAGGA GTACAAGGTT TGGTGTCTGG GCAATGATTC TCAGACACGC 
TACAACATCA ATCAGACGGT GGACGGCAGT GTGTTAAGCA CTGTACTGAA GGGTCTGCTG 
CCTGGCGTCC TGTACCAGGT GGAGGTGGCA GCAGTGACCA GTGCAGGAGT GGGCAAACAC 
AGCCAACCCG TATCTGTTCT TATCAAATTG CCGGCAGAGC AGCCCACAGT CACAGGTGGA 
GGCAGTGAAT CTGAGGAGAA TGTAAGTCTG GCGGAGCAGA TCACAGACGT GGTGAAGCAG 
CCTGCCTTCA TCGCAGGCAT TGGCGGAGCA TGCTGGGTAA TCCTGATGGG ATTCAGCGTG 
TGGATCTACT GCCGACGCAA GAAGAGAAAA GAACTCAGCC ATTACACTGC GTCATTTGCA 
TACACACCGG CAGTTGGTTT TCCACATGGT GAAAGTTCTG GACTTAACGG AAGGCCTGGT 
ATGCTGGGTG CTAACATGGG AAATTATCCA TGGCTGGCTG ATTCTTGGCC TACTACCAAT 
CTTGTGCACA ACGGGAAAGA CTCAGTGAAC TGCTGTACAT CTAAACACGA AACACCGGAG 
AGATACTATA ACGAGGCGGG AATCTCTAAT TACCTCAATC AAAATGAGAA GTACAGCGCA 
GACTCCAACG AGGGGCCGAT CTACAGCACC ATCGACCCCA CCAGCGAAGA CTTACGCAGT 
TTTACCGCTC AGTATTCTCA GCACACCACA CCCTACGCCA GCACTCCCAT TATGCCTTTC 
ACCAACCAGG CGCCCTGCAG CCCTGAGCAA GATGGCAGCC ACTGGATGAC GCAGCCGGGA 
ACCTCCAATG CTCAGTATGC CCAGCCAGAC CGATCCAAGA CTGAGAACGT AAAACAACCA 
AAGCAGAAGT TGATGGGGAA GCCGGTGAAA ACCCCATCTC TCAACTGGAT GGATGCGCTG 
CCGCCTCCAC CCATCTCTGG AGATCTGGAG CAGTGTGACG AGGAGGATGA TAACGAACTA 
ATACAAGACA TGGGGTCTGA TGAGGAAGAG TGGTGTCCTC CTCTTCCCGA GCGGTCTTAT 
CTCATGGACG GCATGGTGGA GGACGGTCCA GTTCCACCAC CAAGAGGAGA AGCTTCTTCA 
CCCACTACGT CCTACAGCCA CCAGTCCACC GCCACCTTAA CCCCTTCTCC CCGAGACGAT 
GGACACTTCC ACGATATACC ACGCTTACAC CAGCTCGAAC AACCACACAT GACACGCCGG 
TTGCCGTGTG GACCAGTGCC TGTGCCACGA GCACCAAGCC CACCTCTAAC CCAATCCAAC 
CCAAACCTGT CAGCCAATCA GGAGTGTCCT GACAACACTG TGCCACACCG TTCAGCTCAT 
AAACGAGACC TGAGCCAAGG AGCTGCACAA AGTGTAGAGA ACATCAGCAA CACCACACCA 
GGTCGAGCGA GGCCCTCCGC AGACCACACA TCGTCTGGGC AGAAATCCAA GGTGAAGAAG 
AAAGCTTCCA AGAGCGCTCA ATACCGGAGA GATATACTGC AGGGCGATCT GCCTCCTCCA 
CCAGAGCCTC CACCCGAAGA CCAGGATAAT TGCGCTCATG TAGGGGGCTT CGAATGCGGG 
CAGACGTCCC TCGAGAGAGC CGGCAGCTCC AATTCGTCCA TGGAGAGGAG AGAGCAGAGC 
ACACCCAACC GGAAAAACTC GGCCCATAGA CGGATAGACG ATGACGATTT AATTCCTTAC 
AACAAGCCTA ACTTCCTGTC CCGAGGTCAG ATGTCCAGCA ATTGCTCCAC AACAGGAAGT 
TCATCATCAC GAGGGTCAAC GGGGTCGCGA GGGCAAGGAA CGGGCAGGAA GAGAAATGAT 
GAGATGAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172694.1
CDS	430..4701
Translation	MLRYLIKTLLQMNLFTDTIRDATNGTDLFFNISSIPSFNSSLLDWANFTGLNGSRPRLEDAAPRIVEHPSDLIVSKGEPATLNCKAEGRPTPMVEWYKDGERVETDREDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYVCVARNYLGEAVSRNASLEVAILRDDFRQTPSDVVVAAGEPAVMECIPPRGHPEPTISWKRNNVKVNDKDERITIRGGKLMISNTRKSDAGMYVCVGTNMVGEKDSDPAELVVFERPMFVRRPVNQVVLADDTVDFQCEVQGDPAPTIRWKKEEGELPRGRFEIRADNSLRLTQVKAEDEGSYTCLSENSVGKAEASGSLQVHVGPSLPPQIVVRPRDQISAQGRTVTFLCGTKGNPPPAVFWQKEGSQVLLFPSQPPSQSGRFSVSLSGELTITDVHSEDSGYYICQAISVAGSILTKALLEVESTPSDRIPPIIRQGPANQTLAPGTTAQLQCHVMGNPLPSIQWERDGQRILGIDERISLMENGTLQITALQETDSGAYTCVASSLSGETSWSGVLTVKESGGLSASPVSEPYQLPGPPQKPVVTDVTRNSVTLTWQPNAHEGGAAVTSYIIEAFSQSAGSTWQTVADFVKLEKHTVTGLSPNTIYLFIVRAVNAYGLSDPSPISEPVRTQDVSPTGQGVDHRQVQRELGEMSVQLHEPVLLTASSVKISWNVDRQSQYIQGYRLLYRPVGGSWSQQEVKGATERSAVIANLLKGTEYEIKIRPYFNEFQGLDSRSQTLRTPEEVPSAPPRAVNVATVKLSNSSSISVSWQPPPTDMQNGVIQEYKVWCLGNDSQTRYNINQTVDGSVLSTVLKGLLPGVLYQVEVAAVTSAGVGKHSQPVSVLIKLPAEQPTVTGGGSESEENVSLAEQITDVVKQPAFIAGIGGACWVILMGFSVWIYCRRKKRKELSHYTASFAYTPAVGFPHGESSGLNGRPGMLGANMGNYPWLADSWPTTNLVHNGKDSVNCCTSKHETPERYYNEAGISNYLNQNEKYSADSNEGPIYSTIDPTSEDLRSFTAQYSQHTTPYASTPIMPFTNQAPCSPEQDGSHWMTQPGTSNAQYAQPDRSKTENVKQPKQKLMGKPVKTPSLNWMDALPPPPISGDLEQCDEEDDNELIQDMGSDEEEWCPPLPERSYLMDGMVEDGPVPPPRGEASSPTTSYSHQSTATLTPSPRDDGHFHDIPRLHQLEQPHMTRRLPCGPVPVPRAPSPPLTQSNPNLSANQECPDNTVPHRSAHKRDLSQGAAQSVENISNTTPGRARPSADHTSSGQKSKVKKKASKSAQYRRDILQGDLPPPPEPPPEDQDNCAHVGGFECGQTSLERAGSSNSSMERREQSTPNRKNSAHRRIDDDDLIPYNKPNFLSRGQMSSNCSTTGSSSSRGSTGSRGQGTGRKRNDEMR

Target ORF information:

RefSeq Version	XM_005172637.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172637.4

ORF Insert Sequence:

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TGGGCCCATC TCTTCCTCCT CAGATTGTAG TGCGTCCGCG GGATCAGATC 
TCTGCCCAGG GCCGCACTGT CACCTTTCTC TGCGGAACGA AGGGAAATCC CCCTCCTGCT 
GTTTTCTGGC AGAAAGAAGG CAGTCAGGTC CTGCTGTTTC CCAGTCAGCC GCCCTCACAG 
TCAGGCCGTT TTTCCGTGTC TCTGAGCGGA GAGCTTACCA TCACAGATGT GCACAGCGAG 
GATTCTGGCT ACTACATCTG CCAGGCTATC AGTGTGGCGG GCAGCATCTT GACCAAAGCA 
CTGCTGGAGG TGGAAAGCAC TCCATCTGAC CGGATCCCTC CCATCATCCG CCAAGGCCCG 
GCCAACCAGA CGCTAGCTCC GGGCACCACG GCCCAATTGC AGTGCCACGT GATGGGCAAC 
CCTCTTCCGA GCATCCAATG GGAGCGCGAC GGACAACGAA TCCTGGGCAT CGACGAGCGC 
ATTAGCCTAA TGGAAAACGG CACTTTGCAA ATCACAGCCC TGCAGGAGAC GGACTCCGGG 
GCCTACACCT GTGTTGCTTC CAGTCTGAGT GGAGAGACGA GCTGGAGTGG TGTGCTCACA 
GTAAAAGAGA GTGGTGGTCT ATCAGCATCT CCGGTATCAG AGCCCTATCA ACTGCCCGGT 
CCTCCTCAGA AACCTGTCGT AACGGATGTA ACCCGTAACA GTGTCACCCT CACCTGGCAA 
CCCAACGCAC ACGAAGGCGG CGCCGCCGTC ACCTCTTACA TCATCGAGGC CTTCAGTCAA 
TCGGCAGGCA GTACGTGGCA GACGGTTGCA GATTTTGTGA AGCTGGAAAA GCACACGGTC 
ACCGGATTGT CTCCGAACAC CATTTACCTG TTTATTGTGC GAGCCGTCAA TGCATATGGC 
CTGAGTGACC CAAGCCCAAT TTCAGAGCCC GTACGCACAC AAGATGTGAG TCCCACAGGT 
CAGGGTGTTG ACCATCGTCA GGTGCAGAGG GAGCTGGGAG AAATGTCTGT ACAGCTTCAC 
GAACCCGTGC TGTTGACGGC CTCCAGTGTG AAGATCAGCT GGAACGTGGA CCGTCAGTCG 
CAGTACATCC AGGGTTATCG GCTGCTTTAT CGGCCTGTCG GCGGTTCCTG GTCCCAGCAG 
GAAGTGAAGG GTGCAACAGA GCGTAGCGCC GTCATCGCTA ACCTGCTCAA AGGCACCGAG 
TACGAGATTA AAATACGACC CTACTTTAAC GAGTTTCAGG GCCTGGACAG CCGATCGCAG 
ACACTCCGCA CGCCAGAGGA GGTGCCGAGT GCTCCTCCTC GAGCTGTGAA TGTGGCCACA 
GTGAAGCTCA GTAACAGCTC TAGCATCAGT GTTTCATGGC AGCCTCCACC CACAGACATG 
CAGAATGGAG TCATTCAGGA GTACAAGGTT TGGTGTCTGG GCAATGATTC TCAGACACGC 
TACAACATCA ATCAGACGGT GGACGGCAGT GTGTTAAGCA CTGTACTGAA GGGTCTGCTG 
CCTGGCGTCC TGTACCAGGT GGAGGTGGCA GCAGTGACCA GTGCAGGAGT GGGCAAACAC 
AGCCAACCCG TATCTGTTCT TATCAAATTG CCGGCAGAGC AGCCCACAGT CACAGGTGGA 
GGCAGTGAAT CTGAGGAGAA TGTAAGTCTG GCGGAGCAGA TCACAGACGT GGTGAAGCAG 
CCTGCCTTCA TCGCAGGCAT TGGCGGAGCA TGCTGGGTAA TCCTGATGGG ATTCAGCGTG 
TGGATCTACT GCCGACGCAA GAAGAGAAAA GAACTCAGCC ATTACACTGC GTCATTTGCA 
TACACACCGG CAGTTGGTTT TCCACATGGT GAAAGTTCTG GACTTAACGG AAGGCCTGGT 
ATGCTGGGTG CTAACATGGG AAATTATCCA TGGCTGGCTG ATTCTTGGCC TACTACCAAT 
CTTGTGCACA ACGGGAAAGA CTCAGTGAAC TGCTGTACAT CTAAACACGA AACACCGGAG 
AGATACTATA ACGAGGCGGG AATCTCTAAT TACCTCAATC AAAATGAGAA GTACAGCGCA 
GACTCCAACG AGGGGCCGAT CTACAGCACC ATCGACCCCA CCAGCGAAGA CTTACGCAGT 
TTTACCGCTC AGTATTCTCA GCACACCACA CCCTACGCCA GCACTCCCAT TATGCCTTTC 
ACCAACCAGG CGCCCTGCAG CCCTGAGCAA GATGGCAGCC ACTGGATGAC GCAGCCGGGA 
ACCTCCAATG CTCAGTATGC CCAGCCAGAC CGATCCAAGA CTGAGAACGT AAAACAACCA 
AAGCAGAAGT TGATGGGGAA GCCGGTGAAA ACCCCATCTC TCAACTGGAT GGATGCGCTG 
CCGCCTCCAC CCATCTCTGG AGATCTGGAG CAGTGTGACG AGGAGGATGA TAACGAACTA 
ATACAAGACA TGGGGTCTGA TGAGGAAGAG TGGTGTCCTC CTCTTCCCGA GCGGTCTTAT 
CTCATGGACG GCATGGTGGA GGACGGTCCA GTTCCACCAC CAAGAGGAGA AGCTTCTTCA 
CCCACTACGT CCTACAGCCA CCAGTCCACC GCCACCTTAA CCCCTTCTCC CCGAGACGAT 
GGACACTTCC ACGATATACC ACGCTTACAC CAGCTCGAAC AACCACACAT GACACGCCGG 
TTGCCGTGTG GACCAGTGCC TGTGCCACGA GCACCAAGCC CACCTCTAAC CCAATCCAAC 
CCAAACCTGT CAGCCAATCA GGAGTGTCCT GACAACACTG TGCCACACCG TTCAGCTCAT 
AAACGAGACC TGAGCCAAGG AGCTGCACAA AGTGTAGAGA ACATCAGCAA CACCACACCA 
GGTCGAGCGA GGCCCTCCGC AGACCACACA TCGTCTGGGC AGAAATCCAA GGTGAAGAAG 
AAAGCTTCCA AGAGCGCTCA ATACCGGAGA GATATACTGC AGGGCGATCT GCCTCCTCCA 
CCAGAGCCTC CACCCGAAGA CCAGGATAAT TGCGCTCATG TAGGGGGCTT CGAATGCGGG 
CAGACGTCCC TCGAGAGAGC CGGCAGCTCC AATTCGTCCA TGGAGAGGAG AGAGCAGAGC 
ACACCCAACC GGAAAAACTC GGCCCATAGA CGGATAGACG ATGACGATTT AATTCCTTAC 
AACAAGCCTA ACTTCCTGTC CCGAGGTCAG ATGTCCAGCA ATTGCTCCAC AACAGGAAGT 
TCATCATCAC GAGGGTCAAC GGGGTCGCGA GGGCAAGGAA CGGGCAGGAA GAGAAATGAT 
GAGATGAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_005172638.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172695.1 Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa16201
Clone ID Related Accession (Same CDS sequence)	XM_005172638.3 , XM_005172638.4
Accession Version	XM_005172638.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172638.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TTCCTCCTCA GATTGTAGTG CGTCCGCGGG ATCAGATCTC TGCCCAGGGC 
CGCACTGTCA CCTTTCTCTG CGGAACGAAG GGAAATCCCC CTCCTGCTGT TTTCTGGCAG 
AAAGAAGGCA GTCAGGTCCT GCTGTTTCCC AGTCAGCCGC CCTCACAGTC AGGCCGTTTT 
TCCGTGTCTC TGAGCGGAGA GCTTACCATC ACAGATGTGC ACAGCGAGGA TTCTGGCTAC 
TACATCTGCC AGGCTATCAG TGTGGCGGGC AGCATCTTGA CCAAAGCACT GCTGGAGGTG 
GAAAGCACTC CATCTGACCG GATCCCTCCC ATCATCCGCC AAGGCCCGGC CAACCAGACG 
CTAGCTCCGG GCACCACGGC CCAATTGCAG TGCCACGTGA TGGGCAACCC TCTTCCGAGC 
ATCCAATGGG AGCGCGACGG ACAACGAATC CTGGGCATCG ACGAGCGCAT TAGCCTAATG 
GAAAACGGCA CTTTGCAAAT CACAGCCCTG CAGGAGACGG ACTCCGGGGC CTACACCTGT 
GTTGCTTCCA GTCTGAGTGG AGAGACGAGC TGGAGTGGTG TGCTCACAGT AAAAGAGAGT 
GGTGGTCTAT CAGCATCTCC GGTATCAGAG CCCTATCAAC TGCCCGGTCC TCCTCAGAAA 
CCTGTCGTAA CGGATGTAAC CCGTAACAGT GTCACCCTCA CCTGGCAACC CAACGCACAC 
GAAGGCGGCG CCGCCGTCAC CTCTTACATC ATCGAGGCCT TCAGTCAATC GGCAGGCAGT 
ACGTGGCAGA CGGTTGCAGA TTTTGTGAAG CTGGAAAAGC ACACGGTCAC CGGATTGTCT 
CCGAACACCA TTTACCTGTT TATTGTGCGA GCCGTCAATG CATATGGCCT GAGTGACCCA 
AGCCCAATTT CAGAGCCCGT ACGCACACAA GATGTGAGTC CCACAGGTCA GGGTGTTGAC 
CATCGTCAGG TGCAGAGGGA GCTGGGAGAA ATGTCTGTAC AGCTTCACGA ACCCGTGCTG 
TTGACGGCCT CCAGTGTGAA GATCAGCTGG AACGTGGACC GTCAGTCGCA GTACATCCAG 
GGTTATCGGC TGCTTTATCG GCCTGTCGGC GGTTCCTGGT CCCAGCAGGA AGTGAAGGGT 
GCAACAGAGC GTAGCGCCGT CATCGCTAAC CTGCTCAAAG GCACCGAGTA CGAGATTAAA 
ATACGACCCT ACTTTAACGA GTTTCAGGGC CTGGACAGCC GATCGCAGAC ACTCCGCACG 
CCAGAGGAGG TGCCGAGTGC TCCTCCTCGA GCTGTGAATG TGGCCACAGT GAAGCTCAGT 
AACAGCTCTA GCATCAGTGT TTCATGGCAG CCTCCACCCA CAGACATGCA GAATGGAGTC 
ATTCAGGAGT ACAAGGTTTG GTGTCTGGGC AATGATTCTC AGACACGCTA CAACATCAAT 
CAGACGGTGG ACGGCAGTGT GTTAAGCACT GTACTGAAGG GTCTGCTGCC TGGCGTCCTG 
TACCAGGTGG AGGTGGCAGC AGTGACCAGT GCAGGAGTGG GCAAACACAG CCAACCCGTA 
TCTGTTCTTA TCAAATTGCC GGCAGAGCAG CCCACAGTCA CAGGTGGAGG CAGTGAATCT 
GAGGAGAATG TAAGTCTGGC GGAGCAGATC ACAGACGTGG TGAAGCAGCC TGCCTTCATC 
GCAGGCATTG GCGGAGCATG CTGGGTAATC CTGATGGGAT TCAGCGTGTG GATCTACTGC 
CGACGCAAGA AGAGAAAAGA ACTCAGCCAT TACACTGCGT CATTTGCATA CACACCGGCA 
GTTGGTTTTC CACATGGTGA AAGTTCTGGA CTTAACGGAA GGCCTGGTAT GCTGGGTGCT 
AACATGGGAA ATTATCCATG GCTGGCTGAT TCTTGGCCTA CTACCAATCT TGTGCACAAC 
GGGAAAGACT CAGTGAACTG CTGTACATCT AAACACGAAA CACCGGAGAG ATACTATAAC 
GAGGCGGGAA TCTCTAATTA CCTCAATCAA AATGAGAAGT ACAGCGCAGA CTCCAACGAG 
GGGCCGATCT ACAGCACCAT CGACCCCACC AGCGAAGACT TACGCAGTTT TACCGCTCAG 
TATTCTCAGC ACACCACACC CTACGCCAGC ACTCCCATTA TGCCTTTCAC CAACCAGGCG 
CCCTGCAGCC CTGAGCAAGA TGGCAGCCAC TGGATGACGC AGCCGGGAAC CTCCAATGCT 
CAGTATGCCC AGCCAGACCG ATCCAAGACT GAGAACGTAA AACAACCAAA GCAGAAGTTG 
ATGGGGAAGC CGGTGAAAAC CCCATCTCTC AACTGGATGG ATGCGCTGCC GCCTCCACCC 
ATCTCTGGAG ATCTGGAGCA GTGTGACGAG GAGGATGATA ACGAACTAAT ACAAGACATG 
GGGTCTGATG AGGAAGAGTG GTGTCCTCCT CTTCCCGAGC GGTCTTATCT CATGGACGGC 
ATGGTGGAGG ACGGTCCAGT TCCACCACCA AGAGGAGAAG CTTCTTCACC CACTACGTCC 
TACAGCCACC AGTCCACCGC CACCTTAACC CCTTCTCCCC GAGACGATGG ACACTTCCAC 
GATATACCAC GCTTACACCA GCTCGAACAA CCACACATGA CACGCCGGTT GCCGTGTGGA 
CCAGTGCCTG TGCCACGAGC ACCAAGCCCA CCTCTAACCC AATCCAACCC AAACCTGTCA 
GCCAATCAGG AGTGTCCTGA CAACACTGTG CCACACCGTT CAGCTCATAA ACGAGACCTG 
AGCCAAGGAG CTGCACAAAG TGTAGAGAAC ATCAGCAACA CCACACCAGG TCGAGCGAGG 
CCCTCCGCAG ACCACACATC GTCTGGGCAG AAATCCAAGG TGAAGAAGAA AGCTTCCAAG 
AGCGCTCAAT ACCGGAGAGA TATACTGCAG GGCGATCTGC CTCCTCCACC AGAGCCTCCA 
CCCGAAGACC AGGATAATTG CGCTCATGTA GGGGGCTTCG AATGCGGGCA GACGTCCCTC 
GAGAGAGCCG GCAGCTCCAA TTCGTCCATG GAGAGGAGAG AGCAGAGCAC ACCCAACCGG 
AAAAACTCGG CCCATAGACG GATAGACGCA GATGACGATT TAATTCCTTA CAACAAGCCT 
AACTTCCTGT CCCGAGGTCA GATGTCCAGC AATTGCTCCA CAACAGGAAG TTCATCATCA 
CGAGGGTCAA CGGGGTCGCG AGGGCAAGGA ACGGGCAGGA AGAGAAATGA TGAGATGAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172695.1
CDS	439..4701
Translation	MLRYLIKTLLQMNLFTDTIRDATNGTDLFFNISSIPSFNSSLLDWANFTGLNGSRPRLEDAAPRIVEHPSDLIVSKGEPATLNCKAEGRPTPMVEWYKDGERVETDREDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYVCVARNYLGEAVSRNASLEVAILRDDFRQTPSDVVVAAGEPAVMECIPPRGHPEPTISWKRNNVKVNDKDERITIRGGKLMISNTRKSDAGMYVCVGTNMVGEKDSDPAELVVFERPMFVRRPVNQVVLADDTVDFQCEVQGDPAPTIRWKKEEGELPRGRFEIRADNSLRLTQVKAEDEGSYTCLSENSVGKAEASGSLQVHVPPQIVVRPRDQISAQGRTVTFLCGTKGNPPPAVFWQKEGSQVLLFPSQPPSQSGRFSVSLSGELTITDVHSEDSGYYICQAISVAGSILTKALLEVESTPSDRIPPIIRQGPANQTLAPGTTAQLQCHVMGNPLPSIQWERDGQRILGIDERISLMENGTLQITALQETDSGAYTCVASSLSGETSWSGVLTVKESGGLSASPVSEPYQLPGPPQKPVVTDVTRNSVTLTWQPNAHEGGAAVTSYIIEAFSQSAGSTWQTVADFVKLEKHTVTGLSPNTIYLFIVRAVNAYGLSDPSPISEPVRTQDVSPTGQGVDHRQVQRELGEMSVQLHEPVLLTASSVKISWNVDRQSQYIQGYRLLYRPVGGSWSQQEVKGATERSAVIANLLKGTEYEIKIRPYFNEFQGLDSRSQTLRTPEEVPSAPPRAVNVATVKLSNSSSISVSWQPPPTDMQNGVIQEYKVWCLGNDSQTRYNINQTVDGSVLSTVLKGLLPGVLYQVEVAAVTSAGVGKHSQPVSVLIKLPAEQPTVTGGGSESEENVSLAEQITDVVKQPAFIAGIGGACWVILMGFSVWIYCRRKKRKELSHYTASFAYTPAVGFPHGESSGLNGRPGMLGANMGNYPWLADSWPTTNLVHNGKDSVNCCTSKHETPERYYNEAGISNYLNQNEKYSADSNEGPIYSTIDPTSEDLRSFTAQYSQHTTPYASTPIMPFTNQAPCSPEQDGSHWMTQPGTSNAQYAQPDRSKTENVKQPKQKLMGKPVKTPSLNWMDALPPPPISGDLEQCDEEDDNELIQDMGSDEEEWCPPLPERSYLMDGMVEDGPVPPPRGEASSPTTSYSHQSTATLTPSPRDDGHFHDIPRLHQLEQPHMTRRLPCGPVPVPRAPSPPLTQSNPNLSANQECPDNTVPHRSAHKRDLSQGAAQSVENISNTTPGRARPSADHTSSGQKSKVKKKASKSAQYRRDILQGDLPPPPEPPPEDQDNCAHVGGFECGQTSLERAGSSNSSMERREQSTPNRKNSAHRRIDADDDLIPYNKPNFLSRGQMSSNCSTTGSSSSRGSTGSRGQGTGRKRNDEMR

Target ORF information:

RefSeq Version	XM_005172638.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172638.3

ORF Insert Sequence:

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TTCCTCCTCA GATTGTAGTG CGTCCGCGGG ATCAGATCTC TGCCCAGGGC 
CGCACTGTCA CCTTTCTCTG CGGAACGAAG GGAAATCCCC CTCCTGCTGT TTTCTGGCAG 
AAAGAAGGCA GTCAGGTCCT GCTGTTTCCC AGTCAGCCGC CCTCACAGTC AGGCCGTTTT 
TCCGTGTCTC TGAGCGGAGA GCTTACCATC ACAGATGTGC ACAGCGAGGA TTCTGGCTAC 
TACATCTGCC AGGCTATCAG TGTGGCGGGC AGCATCTTGA CCAAAGCACT GCTGGAGGTG 
GAAAGCACTC CATCTGACCG GATCCCTCCC ATCATCCGCC AAGGCCCGGC CAACCAGACG 
CTAGCTCCGG GCACCACGGC CCAATTGCAG TGCCACGTGA TGGGCAACCC TCTTCCGAGC 
ATCCAATGGG AGCGCGACGG ACAACGAATC CTGGGCATCG ACGAGCGCAT TAGCCTAATG 
GAAAACGGCA CTTTGCAAAT CACAGCCCTG CAGGAGACGG ACTCCGGGGC CTACACCTGT 
GTTGCTTCCA GTCTGAGTGG AGAGACGAGC TGGAGTGGTG TGCTCACAGT AAAAGAGAGT 
GGTGGTCTAT CAGCATCTCC GGTATCAGAG CCCTATCAAC TGCCCGGTCC TCCTCAGAAA 
CCTGTCGTAA CGGATGTAAC CCGTAACAGT GTCACCCTCA CCTGGCAACC CAACGCACAC 
GAAGGCGGCG CCGCCGTCAC CTCTTACATC ATCGAGGCCT TCAGTCAATC GGCAGGCAGT 
ACGTGGCAGA CGGTTGCAGA TTTTGTGAAG CTGGAAAAGC ACACGGTCAC CGGATTGTCT 
CCGAACACCA TTTACCTGTT TATTGTGCGA GCCGTCAATG CATATGGCCT GAGTGACCCA 
AGCCCAATTT CAGAGCCCGT ACGCACACAA GATGTGAGTC CCACAGGTCA GGGTGTTGAC 
CATCGTCAGG TGCAGAGGGA GCTGGGAGAA ATGTCTGTAC AGCTTCACGA ACCCGTGCTG 
TTGACGGCCT CCAGTGTGAA GATCAGCTGG AACGTGGACC GTCAGTCGCA GTACATCCAG 
GGTTATCGGC TGCTTTATCG GCCTGTCGGC GGTTCCTGGT CCCAGCAGGA AGTGAAGGGT 
GCAACAGAGC GTAGCGCCGT CATCGCTAAC CTGCTCAAAG GCACCGAGTA CGAGATTAAA 
ATACGACCCT ACTTTAACGA GTTTCAGGGC CTGGACAGCC GATCGCAGAC ACTCCGCACG 
CCAGAGGAGG TGCCGAGTGC TCCTCCTCGA GCTGTGAATG TGGCCACAGT GAAGCTCAGT 
AACAGCTCTA GCATCAGTGT TTCATGGCAG CCTCCACCCA CAGACATGCA GAATGGAGTC 
ATTCAGGAGT ACAAGGTTTG GTGTCTGGGC AATGATTCTC AGACACGCTA CAACATCAAT 
CAGACGGTGG ACGGCAGTGT GTTAAGCACT GTACTGAAGG GTCTGCTGCC TGGCGTCCTG 
TACCAGGTGG AGGTGGCAGC AGTGACCAGT GCAGGAGTGG GCAAACACAG CCAACCCGTA 
TCTGTTCTTA TCAAATTGCC GGCAGAGCAG CCCACAGTCA CAGGTGGAGG CAGTGAATCT 
GAGGAGAATG TAAGTCTGGC GGAGCAGATC ACAGACGTGG TGAAGCAGCC TGCCTTCATC 
GCAGGCATTG GCGGAGCATG CTGGGTAATC CTGATGGGAT TCAGCGTGTG GATCTACTGC 
CGACGCAAGA AGAGAAAAGA ACTCAGCCAT TACACTGCGT CATTTGCATA CACACCGGCA 
GTTGGTTTTC CACATGGTGA AAGTTCTGGA CTTAACGGAA GGCCTGGTAT GCTGGGTGCT 
AACATGGGAA ATTATCCATG GCTGGCTGAT TCTTGGCCTA CTACCAATCT TGTGCACAAC 
GGGAAAGACT CAGTGAACTG CTGTACATCT AAACACGAAA CACCGGAGAG ATACTATAAC 
GAGGCGGGAA TCTCTAATTA CCTCAATCAA AATGAGAAGT ACAGCGCAGA CTCCAACGAG 
GGGCCGATCT ACAGCACCAT CGACCCCACC AGCGAAGACT TACGCAGTTT TACCGCTCAG 
TATTCTCAGC ACACCACACC CTACGCCAGC ACTCCCATTA TGCCTTTCAC CAACCAGGCG 
CCCTGCAGCC CTGAGCAAGA TGGCAGCCAC TGGATGACGC AGCCGGGAAC CTCCAATGCT 
CAGTATGCCC AGCCAGACCG ATCCAAGACT GAGAACGTAA AACAACCAAA GCAGAAGTTG 
ATGGGGAAGC CGGTGAAAAC CCCATCTCTC AACTGGATGG ATGCGCTGCC GCCTCCACCC 
ATCTCTGGAG ATCTGGAGCA GTGTGACGAG GAGGATGATA ACGAACTAAT ACAAGACATG 
GGGTCTGATG AGGAAGAGTG GTGTCCTCCT CTTCCCGAGC GGTCTTATCT CATGGACGGC 
ATGGTGGAGG ACGGTCCAGT TCCACCACCA AGAGGAGAAG CTTCTTCACC CACTACGTCC 
TACAGCCACC AGTCCACCGC CACCTTAACC CCTTCTCCCC GAGACGATGG ACACTTCCAC 
GATATACCAC GCTTACACCA GCTCGAACAA CCACACATGA CACGCCGGTT GCCGTGTGGA 
CCAGTGCCTG TGCCACGAGC ACCAAGCCCA CCTCTAACCC AATCCAACCC AAACCTGTCA 
GCCAATCAGG AGTGTCCTGA CAACACTGTG CCACACCGTT CAGCTCATAA ACGAGACCTG 
AGCCAAGGAG CTGCACAAAG TGTAGAGAAC ATCAGCAACA CCACACCAGG TCGAGCGAGG 
CCCTCCGCAG ACCACACATC GTCTGGGCAG AAATCCAAGG TGAAGAAGAA AGCTTCCAAG 
AGCGCTCAAT ACCGGAGAGA TATACTGCAG GGCGATCTGC CTCCTCCACC AGAGCCTCCA 
CCCGAAGACC AGGATAATTG CGCTCATGTA GGGGGCTTCG AATGCGGGCA GACGTCCCTC 
GAGAGAGCCG GCAGCTCCAA TTCGTCCATG GAGAGGAGAG AGCAGAGCAC ACCCAACCGG 
AAAAACTCGG CCCATAGACG GATAGACGCA GATGACGATT TAATTCCTTA CAACAAGCCT 
AACTTCCTGT CCCGAGGTCA GATGTCCAGC AATTGCTCCA CAACAGGAAG TTCATCATCA 
CGAGGGTCAA CGGGGTCGCG AGGGCAAGGA ACGGGCAGGA AGAGAAATGA TGAGATGAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_005172638.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172695.1 Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X3, mRNA.

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CloneID	ODa16201
Clone ID Related Accession (Same CDS sequence)	XM_005172638.3 , XM_005172638.4
Accession Version	XM_005172638.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172638.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TTCCTCCTCA GATTGTAGTG CGTCCGCGGG ATCAGATCTC TGCCCAGGGC 
CGCACTGTCA CCTTTCTCTG CGGAACGAAG GGAAATCCCC CTCCTGCTGT TTTCTGGCAG 
AAAGAAGGCA GTCAGGTCCT GCTGTTTCCC AGTCAGCCGC CCTCACAGTC AGGCCGTTTT 
TCCGTGTCTC TGAGCGGAGA GCTTACCATC ACAGATGTGC ACAGCGAGGA TTCTGGCTAC 
TACATCTGCC AGGCTATCAG TGTGGCGGGC AGCATCTTGA CCAAAGCACT GCTGGAGGTG 
GAAAGCACTC CATCTGACCG GATCCCTCCC ATCATCCGCC AAGGCCCGGC CAACCAGACG 
CTAGCTCCGG GCACCACGGC CCAATTGCAG TGCCACGTGA TGGGCAACCC TCTTCCGAGC 
ATCCAATGGG AGCGCGACGG ACAACGAATC CTGGGCATCG ACGAGCGCAT TAGCCTAATG 
GAAAACGGCA CTTTGCAAAT CACAGCCCTG CAGGAGACGG ACTCCGGGGC CTACACCTGT 
GTTGCTTCCA GTCTGAGTGG AGAGACGAGC TGGAGTGGTG TGCTCACAGT AAAAGAGAGT 
GGTGGTCTAT CAGCATCTCC GGTATCAGAG CCCTATCAAC TGCCCGGTCC TCCTCAGAAA 
CCTGTCGTAA CGGATGTAAC CCGTAACAGT GTCACCCTCA CCTGGCAACC CAACGCACAC 
GAAGGCGGCG CCGCCGTCAC CTCTTACATC ATCGAGGCCT TCAGTCAATC GGCAGGCAGT 
ACGTGGCAGA CGGTTGCAGA TTTTGTGAAG CTGGAAAAGC ACACGGTCAC CGGATTGTCT 
CCGAACACCA TTTACCTGTT TATTGTGCGA GCCGTCAATG CATATGGCCT GAGTGACCCA 
AGCCCAATTT CAGAGCCCGT ACGCACACAA GATGTGAGTC CCACAGGTCA GGGTGTTGAC 
CATCGTCAGG TGCAGAGGGA GCTGGGAGAA ATGTCTGTAC AGCTTCACGA ACCCGTGCTG 
TTGACGGCCT CCAGTGTGAA GATCAGCTGG AACGTGGACC GTCAGTCGCA GTACATCCAG 
GGTTATCGGC TGCTTTATCG GCCTGTCGGC GGTTCCTGGT CCCAGCAGGA AGTGAAGGGT 
GCAACAGAGC GTAGCGCCGT CATCGCTAAC CTGCTCAAAG GCACCGAGTA CGAGATTAAA 
ATACGACCCT ACTTTAACGA GTTTCAGGGC CTGGACAGCC GATCGCAGAC ACTCCGCACG 
CCAGAGGAGG TGCCGAGTGC TCCTCCTCGA GCTGTGAATG TGGCCACAGT GAAGCTCAGT 
AACAGCTCTA GCATCAGTGT TTCATGGCAG CCTCCACCCA CAGACATGCA GAATGGAGTC 
ATTCAGGAGT ACAAGGTTTG GTGTCTGGGC AATGATTCTC AGACACGCTA CAACATCAAT 
CAGACGGTGG ACGGCAGTGT GTTAAGCACT GTACTGAAGG GTCTGCTGCC TGGCGTCCTG 
TACCAGGTGG AGGTGGCAGC AGTGACCAGT GCAGGAGTGG GCAAACACAG CCAACCCGTA 
TCTGTTCTTA TCAAATTGCC GGCAGAGCAG CCCACAGTCA CAGGTGGAGG CAGTGAATCT 
GAGGAGAATG TAAGTCTGGC GGAGCAGATC ACAGACGTGG TGAAGCAGCC TGCCTTCATC 
GCAGGCATTG GCGGAGCATG CTGGGTAATC CTGATGGGAT TCAGCGTGTG GATCTACTGC 
CGACGCAAGA AGAGAAAAGA ACTCAGCCAT TACACTGCGT CATTTGCATA CACACCGGCA 
GTTGGTTTTC CACATGGTGA AAGTTCTGGA CTTAACGGAA GGCCTGGTAT GCTGGGTGCT 
AACATGGGAA ATTATCCATG GCTGGCTGAT TCTTGGCCTA CTACCAATCT TGTGCACAAC 
GGGAAAGACT CAGTGAACTG CTGTACATCT AAACACGAAA CACCGGAGAG ATACTATAAC 
GAGGCGGGAA TCTCTAATTA CCTCAATCAA AATGAGAAGT ACAGCGCAGA CTCCAACGAG 
GGGCCGATCT ACAGCACCAT CGACCCCACC AGCGAAGACT TACGCAGTTT TACCGCTCAG 
TATTCTCAGC ACACCACACC CTACGCCAGC ACTCCCATTA TGCCTTTCAC CAACCAGGCG 
CCCTGCAGCC CTGAGCAAGA TGGCAGCCAC TGGATGACGC AGCCGGGAAC CTCCAATGCT 
CAGTATGCCC AGCCAGACCG ATCCAAGACT GAGAACGTAA AACAACCAAA GCAGAAGTTG 
ATGGGGAAGC CGGTGAAAAC CCCATCTCTC AACTGGATGG ATGCGCTGCC GCCTCCACCC 
ATCTCTGGAG ATCTGGAGCA GTGTGACGAG GAGGATGATA ACGAACTAAT ACAAGACATG 
GGGTCTGATG AGGAAGAGTG GTGTCCTCCT CTTCCCGAGC GGTCTTATCT CATGGACGGC 
ATGGTGGAGG ACGGTCCAGT TCCACCACCA AGAGGAGAAG CTTCTTCACC CACTACGTCC 
TACAGCCACC AGTCCACCGC CACCTTAACC CCTTCTCCCC GAGACGATGG ACACTTCCAC 
GATATACCAC GCTTACACCA GCTCGAACAA CCACACATGA CACGCCGGTT GCCGTGTGGA 
CCAGTGCCTG TGCCACGAGC ACCAAGCCCA CCTCTAACCC AATCCAACCC AAACCTGTCA 
GCCAATCAGG AGTGTCCTGA CAACACTGTG CCACACCGTT CAGCTCATAA ACGAGACCTG 
AGCCAAGGAG CTGCACAAAG TGTAGAGAAC ATCAGCAACA CCACACCAGG TCGAGCGAGG 
CCCTCCGCAG ACCACACATC GTCTGGGCAG AAATCCAAGG TGAAGAAGAA AGCTTCCAAG 
AGCGCTCAAT ACCGGAGAGA TATACTGCAG GGCGATCTGC CTCCTCCACC AGAGCCTCCA 
CCCGAAGACC AGGATAATTG CGCTCATGTA GGGGGCTTCG AATGCGGGCA GACGTCCCTC 
GAGAGAGCCG GCAGCTCCAA TTCGTCCATG GAGAGGAGAG AGCAGAGCAC ACCCAACCGG 
AAAAACTCGG CCCATAGACG GATAGACGCA GATGACGATT TAATTCCTTA CAACAAGCCT 
AACTTCCTGT CCCGAGGTCA GATGTCCAGC AATTGCTCCA CAACAGGAAG TTCATCATCA 
CGAGGGTCAA CGGGGTCGCG AGGGCAAGGA ACGGGCAGGA AGAGAAATGA TGAGATGAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172695.1
CDS	47..4309
Translation	MLRYLIKTLLQMNLFTDTIRDATNGTDLFFNISSIPSFNSSLLDWANFTGLNGSRPRLEDAAPRIVEHPSDLIVSKGEPATLNCKAEGRPTPMVEWYKDGERVETDREDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYVCVARNYLGEAVSRNASLEVAILRDDFRQTPSDVVVAAGEPAVMECIPPRGHPEPTISWKRNNVKVNDKDERITIRGGKLMISNTRKSDAGMYVCVGTNMVGEKDSDPAELVVFERPMFVRRPVNQVVLADDTVDFQCEVQGDPAPTIRWKKEEGELPRGRFEIRADNSLRLTQVKAEDEGSYTCLSENSVGKAEASGSLQVHVPPQIVVRPRDQISAQGRTVTFLCGTKGNPPPAVFWQKEGSQVLLFPSQPPSQSGRFSVSLSGELTITDVHSEDSGYYICQAISVAGSILTKALLEVESTPSDRIPPIIRQGPANQTLAPGTTAQLQCHVMGNPLPSIQWERDGQRILGIDERISLMENGTLQITALQETDSGAYTCVASSLSGETSWSGVLTVKESGGLSASPVSEPYQLPGPPQKPVVTDVTRNSVTLTWQPNAHEGGAAVTSYIIEAFSQSAGSTWQTVADFVKLEKHTVTGLSPNTIYLFIVRAVNAYGLSDPSPISEPVRTQDVSPTGQGVDHRQVQRELGEMSVQLHEPVLLTASSVKISWNVDRQSQYIQGYRLLYRPVGGSWSQQEVKGATERSAVIANLLKGTEYEIKIRPYFNEFQGLDSRSQTLRTPEEVPSAPPRAVNVATVKLSNSSSISVSWQPPPTDMQNGVIQEYKVWCLGNDSQTRYNINQTVDGSVLSTVLKGLLPGVLYQVEVAAVTSAGVGKHSQPVSVLIKLPAEQPTVTGGGSESEENVSLAEQITDVVKQPAFIAGIGGACWVILMGFSVWIYCRRKKRKELSHYTASFAYTPAVGFPHGESSGLNGRPGMLGANMGNYPWLADSWPTTNLVHNGKDSVNCCTSKHETPERYYNEAGISNYLNQNEKYSADSNEGPIYSTIDPTSEDLRSFTAQYSQHTTPYASTPIMPFTNQAPCSPEQDGSHWMTQPGTSNAQYAQPDRSKTENVKQPKQKLMGKPVKTPSLNWMDALPPPPISGDLEQCDEEDDNELIQDMGSDEEEWCPPLPERSYLMDGMVEDGPVPPPRGEASSPTTSYSHQSTATLTPSPRDDGHFHDIPRLHQLEQPHMTRRLPCGPVPVPRAPSPPLTQSNPNLSANQECPDNTVPHRSAHKRDLSQGAAQSVENISNTTPGRARPSADHTSSGQKSKVKKKASKSAQYRRDILQGDLPPPPEPPPEDQDNCAHVGGFECGQTSLERAGSSNSSMERREQSTPNRKNSAHRRIDADDDLIPYNKPNFLSRGQMSSNCSTTGSSSSRGSTGSRGQGTGRKRNDEMR

Target ORF information:

RefSeq Version	XM_005172638.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 3 (Drosophila) (robo3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172638.4

ORF Insert Sequence:

ATGCTGCGTT ACCTGATAAA GACGCTCCTG CAGATGAATC TGTTCACCGA TACCATCCGC 
GATGCCACCA ACGGGACCGA TCTGTTCTTC AACATCTCCT CCATCCCGTC CTTCAACTCG 
TCTCTGCTGG ACTGGGCAAA CTTCACCGGA CTCAATGGAT CCCGGCCTAG GTTGGAAGAT 
GCTGCACCCC GGATTGTTGA ACACCCCTCA GACCTCATTG TTTCCAAAGG TGAGCCTGCA 
ACTTTGAACT GTAAGGCCGA AGGACGACCG ACCCCCATGG TGGAGTGGTA CAAAGATGGC 
GAGCGAGTGG AAACCGACCG GGAGGATCCA CGCTCCCACC GCATGCTCCT CCCCAGCGGC 
TCCCTCTTCT TTCTCCGAAT TGTTCACGGA AGGCGCAGCA AGCCAGATGA AGGCGTGTAC 
GTGTGCGTAG CAAGGAATTA CTTGGGCGAG GCCGTCAGTC GTAATGCCTC TTTGGAGGTC 
GCAATTCTCC GGGATGATTT CCGACAGACC CCCAGTGATG TGGTGGTGGC GGCAGGAGAG 
CCGGCAGTGA TGGAGTGCAT CCCTCCACGA GGACACCCAG AACCCACCAT CTCCTGGAAG 
AGGAACAATG TGAAAGTCAA CGACAAGGAC GAGAGGATAA CGATCCGAGG AGGGAAGCTG 
ATGATTTCTA ACACGCGCAA AAGTGATGCC GGGATGTATG TCTGTGTCGG CACCAATATG 
GTTGGAGAGA AGGACAGCGA TCCTGCTGAG CTTGTAGTGT TTGAGCGGCC CATGTTTGTG 
CGCAGACCTG TGAATCAGGT GGTTCTTGCT GATGATACAG TGGATTTCCA GTGTGAGGTT 
CAAGGAGACC CAGCTCCTAC CATACGCTGG AAGAAAGAGG AAGGAGAACT GCCTCGGGGA 
AGGTTTGAGA TCCGTGCCGA CAACAGCCTG CGTCTGACTC AGGTGAAGGC TGAAGATGAA 
GGCTCCTACA CCTGTTTATC AGAGAACAGC GTGGGCAAAG CTGAAGCATC CGGATCGCTT 
CAGGTCCACG TTCCTCCTCA GATTGTAGTG CGTCCGCGGG ATCAGATCTC TGCCCAGGGC 
CGCACTGTCA CCTTTCTCTG CGGAACGAAG GGAAATCCCC CTCCTGCTGT TTTCTGGCAG 
AAAGAAGGCA GTCAGGTCCT GCTGTTTCCC AGTCAGCCGC CCTCACAGTC AGGCCGTTTT 
TCCGTGTCTC TGAGCGGAGA GCTTACCATC ACAGATGTGC ACAGCGAGGA TTCTGGCTAC 
TACATCTGCC AGGCTATCAG TGTGGCGGGC AGCATCTTGA CCAAAGCACT GCTGGAGGTG 
GAAAGCACTC CATCTGACCG GATCCCTCCC ATCATCCGCC AAGGCCCGGC CAACCAGACG 
CTAGCTCCGG GCACCACGGC CCAATTGCAG TGCCACGTGA TGGGCAACCC TCTTCCGAGC 
ATCCAATGGG AGCGCGACGG ACAACGAATC CTGGGCATCG ACGAGCGCAT TAGCCTAATG 
GAAAACGGCA CTTTGCAAAT CACAGCCCTG CAGGAGACGG ACTCCGGGGC CTACACCTGT 
GTTGCTTCCA GTCTGAGTGG AGAGACGAGC TGGAGTGGTG TGCTCACAGT AAAAGAGAGT 
GGTGGTCTAT CAGCATCTCC GGTATCAGAG CCCTATCAAC TGCCCGGTCC TCCTCAGAAA 
CCTGTCGTAA CGGATGTAAC CCGTAACAGT GTCACCCTCA CCTGGCAACC CAACGCACAC 
GAAGGCGGCG CCGCCGTCAC CTCTTACATC ATCGAGGCCT TCAGTCAATC GGCAGGCAGT 
ACGTGGCAGA CGGTTGCAGA TTTTGTGAAG CTGGAAAAGC ACACGGTCAC CGGATTGTCT 
CCGAACACCA TTTACCTGTT TATTGTGCGA GCCGTCAATG CATATGGCCT GAGTGACCCA 
AGCCCAATTT CAGAGCCCGT ACGCACACAA GATGTGAGTC CCACAGGTCA GGGTGTTGAC 
CATCGTCAGG TGCAGAGGGA GCTGGGAGAA ATGTCTGTAC AGCTTCACGA ACCCGTGCTG 
TTGACGGCCT CCAGTGTGAA GATCAGCTGG AACGTGGACC GTCAGTCGCA GTACATCCAG 
GGTTATCGGC TGCTTTATCG GCCTGTCGGC GGTTCCTGGT CCCAGCAGGA AGTGAAGGGT 
GCAACAGAGC GTAGCGCCGT CATCGCTAAC CTGCTCAAAG GCACCGAGTA CGAGATTAAA 
ATACGACCCT ACTTTAACGA GTTTCAGGGC CTGGACAGCC GATCGCAGAC ACTCCGCACG 
CCAGAGGAGG TGCCGAGTGC TCCTCCTCGA GCTGTGAATG TGGCCACAGT GAAGCTCAGT 
AACAGCTCTA GCATCAGTGT TTCATGGCAG CCTCCACCCA CAGACATGCA GAATGGAGTC 
ATTCAGGAGT ACAAGGTTTG GTGTCTGGGC AATGATTCTC AGACACGCTA CAACATCAAT 
CAGACGGTGG ACGGCAGTGT GTTAAGCACT GTACTGAAGG GTCTGCTGCC TGGCGTCCTG 
TACCAGGTGG AGGTGGCAGC AGTGACCAGT GCAGGAGTGG GCAAACACAG CCAACCCGTA 
TCTGTTCTTA TCAAATTGCC GGCAGAGCAG CCCACAGTCA CAGGTGGAGG CAGTGAATCT 
GAGGAGAATG TAAGTCTGGC GGAGCAGATC ACAGACGTGG TGAAGCAGCC TGCCTTCATC 
GCAGGCATTG GCGGAGCATG CTGGGTAATC CTGATGGGAT TCAGCGTGTG GATCTACTGC 
CGACGCAAGA AGAGAAAAGA ACTCAGCCAT TACACTGCGT CATTTGCATA CACACCGGCA 
GTTGGTTTTC CACATGGTGA AAGTTCTGGA CTTAACGGAA GGCCTGGTAT GCTGGGTGCT 
AACATGGGAA ATTATCCATG GCTGGCTGAT TCTTGGCCTA CTACCAATCT TGTGCACAAC 
GGGAAAGACT CAGTGAACTG CTGTACATCT AAACACGAAA CACCGGAGAG ATACTATAAC 
GAGGCGGGAA TCTCTAATTA CCTCAATCAA AATGAGAAGT ACAGCGCAGA CTCCAACGAG 
GGGCCGATCT ACAGCACCAT CGACCCCACC AGCGAAGACT TACGCAGTTT TACCGCTCAG 
TATTCTCAGC ACACCACACC CTACGCCAGC ACTCCCATTA TGCCTTTCAC CAACCAGGCG 
CCCTGCAGCC CTGAGCAAGA TGGCAGCCAC TGGATGACGC AGCCGGGAAC CTCCAATGCT 
CAGTATGCCC AGCCAGACCG ATCCAAGACT GAGAACGTAA AACAACCAAA GCAGAAGTTG 
ATGGGGAAGC CGGTGAAAAC CCCATCTCTC AACTGGATGG ATGCGCTGCC GCCTCCACCC 
ATCTCTGGAG ATCTGGAGCA GTGTGACGAG GAGGATGATA ACGAACTAAT ACAAGACATG 
GGGTCTGATG AGGAAGAGTG GTGTCCTCCT CTTCCCGAGC GGTCTTATCT CATGGACGGC 
ATGGTGGAGG ACGGTCCAGT TCCACCACCA AGAGGAGAAG CTTCTTCACC CACTACGTCC 
TACAGCCACC AGTCCACCGC CACCTTAACC CCTTCTCCCC GAGACGATGG ACACTTCCAC 
GATATACCAC GCTTACACCA GCTCGAACAA CCACACATGA CACGCCGGTT GCCGTGTGGA 
CCAGTGCCTG TGCCACGAGC ACCAAGCCCA CCTCTAACCC AATCCAACCC AAACCTGTCA 
GCCAATCAGG AGTGTCCTGA CAACACTGTG CCACACCGTT CAGCTCATAA ACGAGACCTG 
AGCCAAGGAG CTGCACAAAG TGTAGAGAAC ATCAGCAACA CCACACCAGG TCGAGCGAGG 
CCCTCCGCAG ACCACACATC GTCTGGGCAG AAATCCAAGG TGAAGAAGAA AGCTTCCAAG 
AGCGCTCAAT ACCGGAGAGA TATACTGCAG GGCGATCTGC CTCCTCCACC AGAGCCTCCA 
CCCGAAGACC AGGATAATTG CGCTCATGTA GGGGGCTTCG AATGCGGGCA GACGTCCCTC 
GAGAGAGCCG GCAGCTCCAA TTCGTCCATG GAGAGGAGAG AGCAGAGCAC ACCCAACCGG 
AAAAACTCGG CCCATAGACG GATAGACGCA GATGACGATT TAATTCCTTA CAACAAGCCT 
AACTTCCTGT CCCGAGGTCA GATGTCCAGC AATTGCTCCA CAACAGGAAG TTCATCATCA 
CGAGGGTCAA CGGGGTCGCG AGGGCAAGGA ACGGGCAGGA AGAGAAATGA TGAGATGAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	robo3
Entrez Gene ID	30770
Full Name	roundabout, axon guidance receptor, homolog 3 (Drosophila)
Synonyms	cb503
General protein information	Preferred Names roundabout, axon guidance receptor, homolog 3 (Drosophila) Names roundabout homolog 3 roundabout (axon guidance receptor, Drosophila) homolog 3 twi twitch twice
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Pan troglodytes (chimpanzee)	ROBO3	XP_003313454.2
Canis lupus familiaris (dog)	ROBO3	XP_005619673.1
Danio rerio (zebrafish)	robo3	NP_571557.1
	ROBO3	XP_001106913.2
Mus musculus (house mouse)	Robo3	NP_001158239.1
Bos taurus (cattle)	ROBO3	XP_002699249.1
Rattus norvegicus (Norway rat)	Robo3	NP_001101605.1
Gallus gallus (chicken)	ROBO3	XP_003642645.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010970	XP_309724.3
Homo sapiens (human)	ROBO3	NP_071765.2
Xenopus tropicalis (tropical clawed frog)	robo3	XP_002937567.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

robo3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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