wnk2 cDNA ORF clone, Danio rerio(zebrafish)

The following wnk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa148043 XM_021479885.1
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa148044 XM_021479886.1
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa148045 XM_021479887.1
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa148046 XM_021479888.1
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa53084 XM_009306231.2
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ODa53084 XM_009306230.2
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ODa53085 XM_009306232.2
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ODa53086 XM_009306233.2
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ODa53087 XM_009306234.2
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ODa53088 XM_009306235.2
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ODa53089 XM_009306236.2
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ODa53090 XM_009306237.2
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ODa53091 XM_021479884.1
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa53091 XM_009306238.3
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa54383 XM_017358364.1
Latest version!
Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
View Old Accession Versions >>
ODa53091 XM_009306238.2 Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa148043
    Clone ID Related Accession (Same CDS sequence) XM_021479885.1
    Accession Version XM_021479885.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGTTTCAT CTCTATTTCC ATCCTGAAGC TTTCCTGCCT
    CAGACTTTAT CAACTCTCCA GCCACTAATG CAGGCATCCA CAGATGGTCA GTCTATTCCA
    CAGCATCTAT TACAGTCAGC ATCTTCAATG CTCAGTACCC TACCTCCACA ACCACAACTA
    AATATTCAGA CACCCCTTCT CCAGCCTCTA CAAATTTCCA CAAAGTTTCC TTCGACATAT
    CCTCTTTTAC CAGAAGGGGG CACATCTACA GGGACAGAAC CTATATCTTT CTCCTCAGTC
    TCTTACTCTT CTCCTTATCC CACAAATGCT CCTCCTGCAT CATCCCCCTA CTACTCACCA
    AGCCCCATCA GCGCTCCCCT TCCCATAATA CCCATGCAGA ATGTACCTTG CATACTAGGA
    TCAGGCACAC CTGTATCCAC CCCTCTCAAT GTTCCCACCC CTATACCTCT CCTGGCTATG
    GCCCTCTCCC CACCCATGCC TCCACCAGAG CAACATCTGC AACAGATGTT CTCCCCAATT
    CCAACACCAG AAGGTGCCCT TTACCAACCT CAACCCCAAC CAACACAGCC CTCCCTTCCC
    CTTCCAAATC CAGCATCTCT CCCTCAGCAA GACCCGTCTA TTCCTGAACC TTTTAAAGAG
    GAATACTCTC ACCATGAGGA ATTGCAGATC CTGGCTCCAT CTCACACTGT TATGTCACAG
    ATTCAGTCTC CTCCACAGGA CAGTAAGTCA GAAACAAAGT TTACCAATGT CCAAGCCTTT
    TCTGAGACAG TGGAGCATCC TCTGCAGTCT ACTGGCATTC AGAGAGCTTA TGCTGTTCCT
    GAGAGTACTG GCACAGCTAG TGCAACTCAG CAAAGGGCAG AAACTTCTGC AATGCCCAAA
    CTCCCAGCAC CAGAGCCCTG CCCATTTCAA CACAGCACTG AGGCACCTGC ATCTATTCCT
    CTCCATGCTT TTGTGTGCGA CAGCCTAAAT CAAGATGCAG GATCTAGTAA AGAAATGAGT
    GACAGCTATG AAGGATCCAC CGGTGGAGGA AAAGGTGATG GCAAACCCAG AAAGCATCAC
    CGCAAATCTG CTCGCACACG TTCACGCCAA GAAAAAATTA ACAAGCCAAA ACTAAGCATG
    TTAAATGTTA GTAATACGGG TGACAAGATG GTGGAATGCC AGTTGGAGAC ACACAATCAT
    AAAATGGTGA CTTTCAAGTT CGACCTAGAT GGAGATGCGC CTGAAGAAAT AGCCACTTAT
    ATGGTGGAAA ATGGTTTCAT TCTACCAATA GAAAAGGAGA TTTTCATAGA TCAACTAAAA
    GACATTGTTG ACAAGGCAGA GGATATTTTA AGTGAGGACA TGGATGGAGA GAAGACATCA
    GAACTTGGAA AAGGACAGAC ACCTGGAGAT AAAGGAAGAG AGGCAATAAA TGGACAGCAA
    CCTGGGATTC CCCAGGCAGT TTATCAGCAA AATGTTCTGC ACACAGGCAA GAGGTGGTTC
    ATCATCTGCC CTGTAGAAGA AGCTCCTGCA GCAAGTCAGG ATGGATGCTC AGATGGAGGT
    CCATCTACAC AATCTCCATC TACAACAACA ATAACTGAAG GAACAGCCAC TGCTCAGCCT
    GTAGAGAGTG CATCAGTACA ACCACCAGAA CCCAGCACAG GATCTGCTTC CGCTTCTATG
    GATTCAGAAA CCTGTGCCAC AGCGCCTCCA TCTGGAGGAA GTGATCCCTA TGGGGTCTGG
    AGCCCTCTCT CTTTGACAAA TACTGATCCT CTTTCTCTGG CTGCTCTTTC CCAAGCTCCT
    CCTGCCCCGC AAGCAGCTAT GATGCCAGCA CAGCCTGCTT CACATTCAGA GGAGGGCCAA
    AATTTGGGCT CTACCCAGGT CAGTGTACAG CAGTCCCAGC CCTGCATAGA TCCTCAAAGC
    TCTCTTTTTG TGGAAGAGAC TCAAAGTGGC AGATTAGACT CGGTCTCCGC AATGCACACC
    GCTCAGCAAA TGGCTGAAAT TGCATGTGCT GCCTCATTGG TGGAGGACGT GCCCTGCTGT
    CCCTTGGTCA TGCCGTTGTC CCTTGAAGTA AGCAGCGGGG CTCAGAAGCC ATCAACCGTA
    ATTCCGCCAC CATCACAAGA CAGTACATCT GCTCGCGAAC AACTCCAATC CATTACATCT
    AATCGAGCGG AGCGTGGCCT GCAACCTGTG GTGCTGCAGC AGCCTGTTTC CACTGTAAGT
    GGATCTAAGG CTCCTTCCCT GCCTCAGAGC CCTGCTCCTT CACAGCACCA CTTTGTCCCT
    GGTGAGTCAG ATGGAGAGGG GCGTAGCAGA GGAGGGTTTG TAGACAGCAC TATCAAGACT
    CTGGATGAGA AATTGAGGAA TTTATTGTAC CAAGAGTACG CTCCGATGTA CCCATCGGGA
    AGTGCAGCTG AAACTCCAGG ATCTGGTACA GAGTATATCC AGTCTCCACC AGGACCTGAG
    AGTGCCATGG GAGGGTCAGG AACCAGCACA CCTAGTCTTA TAGGAGAGGG ACGATTCAGG
    GCAGGTGAAC AGCTGCCACA GATCCCAGAG CGGGTAGACA GTTTAAGCGC TCTCAGCGAC
    TCTGCAGTTG GAGTTACTGT GTCAAGGAGA CATGTGATGC CACACTCTAC ATCTTGTTCT
    GGATCTAGAA ATCGATATAA GATGGTGCCA AGCTCCACTG ACATCTTGAC AGGTCAAGGT
    CGAAAGCAGC GCAGCCTGAG CAGCACGGCA TCTCCAGCAC ACCCTGGTGG ATTTTTGGGA
    GAATGTACCA TGTACGAAGA GCCGACTATG TCAGCTACTA CAGTTGGCAG GTTCTCAGTG
    GTCAGCACAG AAGACGAGGT CACGCGTAGA AAGTACAGTA GCAGATATTC AGCTCCACCT
    GACTTTTATC TGGACGCTCC ACCTCCACTG ACCAAACGGG GGTCTTTGCC AAGGGCGCAG
    ACCTCGACTT CAGCAGATGC CACAGTTTGC ACACGATTTA TGTCATCTGA CTCTGGGGCA
    GAAAGCAGCC CTGCAAAAAT GGCACCTACA ACCCCATCCC GGCATGGCAG GTCTGAAAGG
    AGAGGAAGCG ACCTCATGAA AAGGGCAGTG GCTTTTCTAA AGCGCTCTGG CCGCAGCAGT
    AGTGTGCAAA GCTCTGATTC ACCAAGCCGG AAAGGAGGGG TGTATGGATC CTATGTCAGC
    AGTGACAATG ACTCAGAGAT GGAGGACTCA GATATTAAAA AGGAGCTTCA AAATCTAAGA
    GAAAAGCATA TGAAGGAGAT AAGTGAATTG GAGGCCCACC ACAGAGAAGA GATTGAGCTT
    CTCTATGTCA GATTAGGAAA ACCACCACCT CCAGGTCTAT ACATCCCACC CACAGTGCCC
    CCTGCTGGAC GCAAGCGCAG GACCAGCAGG CACAAACTAA AAGCTGGCAA ACTGCTCAGC
    CCTCTAGTAC AACAACTGAG AAATGTTGCT TCTAAAACTG GTGACTCCAG CAAACCTAAT
    GATTTAACCA AATCTGCTGA GGCGGTCCCG AGCTTGAATG GATCTCCTGT CAGAGCATCC
    AACTTGTCTG ATGGCAGGCC TTATTCTGGG ACCGGGACTC TGCCTTGTTC TGTTTCTGAG
    CCAGTTCAGA CCCAACAGCC CTGCTCTCTT AAAGGATCTC TGTCTTCCGA CAATATCTAC
    TATGGTGCAC AGGGAGAAGG ACCCGTGCCA CGCAGCCAGC CTGCCCAAGG CTGGCCTCCT
    TCTCCCCAGG CGTCTGCGCA GGTCACCTAT AAATCCAGTA GTAAACCACG CGCTAGATTC
    CTCAGGTCAA CACTTAAGCG CCTGTGTCTG GGTAAAGAAC GTGGCAGCAG ATCTGGAGCA
    TCATCAGGTG CCTCAAATCA ATCCCCTATG CCTCCTGGAT CCACTCCTCC TCCTCACCAG
    CCTGTTGGCC TTGCCCAAGC ACAGACAAAC AACAGCAACA ACAAAACAGA TGCTTTCACT
    CAGCAGCTTC ACCGACTGGT GGATAACTGG GCAGAAGGTG TGAGGGCTCC TTCACGCTCA
    CGCTCCCTCA GTCTAAGACC ACAGCAACTG ACCAGAACCA GGATCTGGAG TGCAACAGAG
    GTTCCAGGGT CAATGGAAAG ATTGAATGCT TCCCGGCTGC CTCTTTCATG GCCACAGATT
    GATTTCCATG CCTCTGTGAT GGTGACTGAC ACTTCACAAG TGCTCCAGCA CAGTTTTATG
    GTGTCCAGTA ACCCTTATGA AAAGATGCTT AACTCCACGC ACTTTGACAA GGACCTTTGG
    CCAGCAATGA CTGCCAATCT TAACCAAGAA GTCTTTGCTT TCTCTGCAGC GGACTCTCCG
    TCCTGGACAG CACCTTCTTC CCCCAGCGAG GGTCCTAGTT CTAGAAATAG GACCATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335560.1
    CDS694..7413
    Translation

    Target ORF information:

    RefSeq Version XM_021479885.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479885.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGTTTCAT CTCTATTTCC ATCCTGAAGC TTTCCTGCCT
    CAGACTTTAT CAACTCTCCA GCCACTAATG CAGGCATCCA CAGATGGTCA GTCTATTCCA
    CAGCATCTAT TACAGTCAGC ATCTTCAATG CTCAGTACCC TACCTCCACA ACCACAACTA
    AATATTCAGA CACCCCTTCT CCAGCCTCTA CAAATTTCCA CAAAGTTTCC TTCGACATAT
    CCTCTTTTAC CAGAAGGGGG CACATCTACA GGGACAGAAC CTATATCTTT CTCCTCAGTC
    TCTTACTCTT CTCCTTATCC CACAAATGCT CCTCCTGCAT CATCCCCCTA CTACTCACCA
    AGCCCCATCA GCGCTCCCCT TCCCATAATA CCCATGCAGA ATGTACCTTG CATACTAGGA
    TCAGGCACAC CTGTATCCAC CCCTCTCAAT GTTCCCACCC CTATACCTCT CCTGGCTATG
    GCCCTCTCCC CACCCATGCC TCCACCAGAG CAACATCTGC AACAGATGTT CTCCCCAATT
    CCAACACCAG AAGGTGCCCT TTACCAACCT CAACCCCAAC CAACACAGCC CTCCCTTCCC
    CTTCCAAATC CAGCATCTCT CCCTCAGCAA GACCCGTCTA TTCCTGAACC TTTTAAAGAG
    GAATACTCTC ACCATGAGGA ATTGCAGATC CTGGCTCCAT CTCACACTGT TATGTCACAG
    ATTCAGTCTC CTCCACAGGA CAGTAAGTCA GAAACAAAGT TTACCAATGT CCAAGCCTTT
    TCTGAGACAG TGGAGCATCC TCTGCAGTCT ACTGGCATTC AGAGAGCTTA TGCTGTTCCT
    GAGAGTACTG GCACAGCTAG TGCAACTCAG CAAAGGGCAG AAACTTCTGC AATGCCCAAA
    CTCCCAGCAC CAGAGCCCTG CCCATTTCAA CACAGCACTG AGGCACCTGC ATCTATTCCT
    CTCCATGCTT TTGTGTGCGA CAGCCTAAAT CAAGATGCAG GATCTAGTAA AGAAATGAGT
    GACAGCTATG AAGGATCCAC CGGTGGAGGA AAAGGTGATG GCAAACCCAG AAAGCATCAC
    CGCAAATCTG CTCGCACACG TTCACGCCAA GAAAAAATTA ACAAGCCAAA ACTAAGCATG
    TTAAATGTTA GTAATACGGG TGACAAGATG GTGGAATGCC AGTTGGAGAC ACACAATCAT
    AAAATGGTGA CTTTCAAGTT CGACCTAGAT GGAGATGCGC CTGAAGAAAT AGCCACTTAT
    ATGGTGGAAA ATGGTTTCAT TCTACCAATA GAAAAGGAGA TTTTCATAGA TCAACTAAAA
    GACATTGTTG ACAAGGCAGA GGATATTTTA AGTGAGGACA TGGATGGAGA GAAGACATCA
    GAACTTGGAA AAGGACAGAC ACCTGGAGAT AAAGGAAGAG AGGCAATAAA TGGACAGCAA
    CCTGGGATTC CCCAGGCAGT TTATCAGCAA AATGTTCTGC ACACAGGCAA GAGGTGGTTC
    ATCATCTGCC CTGTAGAAGA AGCTCCTGCA GCAAGTCAGG ATGGATGCTC AGATGGAGGT
    CCATCTACAC AATCTCCATC TACAACAACA ATAACTGAAG GAACAGCCAC TGCTCAGCCT
    GTAGAGAGTG CATCAGTACA ACCACCAGAA CCCAGCACAG GATCTGCTTC CGCTTCTATG
    GATTCAGAAA CCTGTGCCAC AGCGCCTCCA TCTGGAGGAA GTGATCCCTA TGGGGTCTGG
    AGCCCTCTCT CTTTGACAAA TACTGATCCT CTTTCTCTGG CTGCTCTTTC CCAAGCTCCT
    CCTGCCCCGC AAGCAGCTAT GATGCCAGCA CAGCCTGCTT CACATTCAGA GGAGGGCCAA
    AATTTGGGCT CTACCCAGGT CAGTGTACAG CAGTCCCAGC CCTGCATAGA TCCTCAAAGC
    TCTCTTTTTG TGGAAGAGAC TCAAAGTGGC AGATTAGACT CGGTCTCCGC AATGCACACC
    GCTCAGCAAA TGGCTGAAAT TGCATGTGCT GCCTCATTGG TGGAGGACGT GCCCTGCTGT
    CCCTTGGTCA TGCCGTTGTC CCTTGAAGTA AGCAGCGGGG CTCAGAAGCC ATCAACCGTA
    ATTCCGCCAC CATCACAAGA CAGTACATCT GCTCGCGAAC AACTCCAATC CATTACATCT
    AATCGAGCGG AGCGTGGCCT GCAACCTGTG GTGCTGCAGC AGCCTGTTTC CACTGTAAGT
    GGATCTAAGG CTCCTTCCCT GCCTCAGAGC CCTGCTCCTT CACAGCACCA CTTTGTCCCT
    GGTGAGTCAG ATGGAGAGGG GCGTAGCAGA GGAGGGTTTG TAGACAGCAC TATCAAGACT
    CTGGATGAGA AATTGAGGAA TTTATTGTAC CAAGAGTACG CTCCGATGTA CCCATCGGGA
    AGTGCAGCTG AAACTCCAGG ATCTGGTACA GAGTATATCC AGTCTCCACC AGGACCTGAG
    AGTGCCATGG GAGGGTCAGG AACCAGCACA CCTAGTCTTA TAGGAGAGGG ACGATTCAGG
    GCAGGTGAAC AGCTGCCACA GATCCCAGAG CGGGTAGACA GTTTAAGCGC TCTCAGCGAC
    TCTGCAGTTG GAGTTACTGT GTCAAGGAGA CATGTGATGC CACACTCTAC ATCTTGTTCT
    GGATCTAGAA ATCGATATAA GATGGTGCCA AGCTCCACTG ACATCTTGAC AGGTCAAGGT
    CGAAAGCAGC GCAGCCTGAG CAGCACGGCA TCTCCAGCAC ACCCTGGTGG ATTTTTGGGA
    GAATGTACCA TGTACGAAGA GCCGACTATG TCAGCTACTA CAGTTGGCAG GTTCTCAGTG
    GTCAGCACAG AAGACGAGGT CACGCGTAGA AAGTACAGTA GCAGATATTC AGCTCCACCT
    GACTTTTATC TGGACGCTCC ACCTCCACTG ACCAAACGGG GGTCTTTGCC AAGGGCGCAG
    ACCTCGACTT CAGCAGATGC CACAGTTTGC ACACGATTTA TGTCATCTGA CTCTGGGGCA
    GAAAGCAGCC CTGCAAAAAT GGCACCTACA ACCCCATCCC GGCATGGCAG GTCTGAAAGG
    AGAGGAAGCG ACCTCATGAA AAGGGCAGTG GCTTTTCTAA AGCGCTCTGG CCGCAGCAGT
    AGTGTGCAAA GCTCTGATTC ACCAAGCCGG AAAGGAGGGG TGTATGGATC CTATGTCAGC
    AGTGACAATG ACTCAGAGAT GGAGGACTCA GATATTAAAA AGGAGCTTCA AAATCTAAGA
    GAAAAGCATA TGAAGGAGAT AAGTGAATTG GAGGCCCACC ACAGAGAAGA GATTGAGCTT
    CTCTATGTCA GATTAGGAAA ACCACCACCT CCAGGTCTAT ACATCCCACC CACAGTGCCC
    CCTGCTGGAC GCAAGCGCAG GACCAGCAGG CACAAACTAA AAGCTGGCAA ACTGCTCAGC
    CCTCTAGTAC AACAACTGAG AAATGTTGCT TCTAAAACTG GTGACTCCAG CAAACCTAAT
    GATTTAACCA AATCTGCTGA GGCGGTCCCG AGCTTGAATG GATCTCCTGT CAGAGCATCC
    AACTTGTCTG ATGGCAGGCC TTATTCTGGG ACCGGGACTC TGCCTTGTTC TGTTTCTGAG
    CCAGTTCAGA CCCAACAGCC CTGCTCTCTT AAAGGATCTC TGTCTTCCGA CAATATCTAC
    TATGGTGCAC AGGGAGAAGG ACCCGTGCCA CGCAGCCAGC CTGCCCAAGG CTGGCCTCCT
    TCTCCCCAGG CGTCTGCGCA GGTCACCTAT AAATCCAGTA GTAAACCACG CGCTAGATTC
    CTCAGGTCAA CACTTAAGCG CCTGTGTCTG GGTAAAGAAC GTGGCAGCAG ATCTGGAGCA
    TCATCAGGTG CCTCAAATCA ATCCCCTATG CCTCCTGGAT CCACTCCTCC TCCTCACCAG
    CCTGTTGGCC TTGCCCAAGC ACAGACAAAC AACAGCAACA ACAAAACAGA TGCTTTCACT
    CAGCAGCTTC ACCGACTGGT GGATAACTGG GCAGAAGGTG TGAGGGCTCC TTCACGCTCA
    CGCTCCCTCA GTCTAAGACC ACAGCAACTG ACCAGAACCA GGATCTGGAG TGCAACAGAG
    GTTCCAGGGT CAATGGAAAG ATTGAATGCT TCCCGGCTGC CTCTTTCATG GCCACAGATT
    GATTTCCATG CCTCTGTGAT GGTGACTGAC ACTTCACAAG TGCTCCAGCA CAGTTTTATG
    GTGTCCAGTA ACCCTTATGA AAAGATGCTT AACTCCACGC ACTTTGACAA GGACCTTTGG
    CCAGCAATGA CTGCCAATCT TAACCAAGAA GTCTTTGCTT TCTCTGCAGC GGACTCTCCG
    TCCTGGACAG CACCTTCTTC CCCCAGCGAG GGTCCTAGTT CTAGAAATAG GACCATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148044
    Clone ID Related Accession (Same CDS sequence) XM_021479886.1
    Accession Version XM_021479886.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGACTTTA TCAACTCTCC AGCCACTAAT GCAGGCATCC
    ACAGATGGTC AGTCTATTCC ACAGCATCTA TTACAGTCAG CATCTTCAAT GCTCAGTACC
    CTACCTCCAC AACCACAACT AAATATTCAG ACACCCCTTC TCCAGCCTCT ACAAATTTCC
    ACAAAGTTTC CTTCGACATA TCCTCTTTTA CCAGAAGGGG GCACATCTAC AGGGACAGAA
    CCTATATCTT TCTCCTCAGT CTCTTACTCT TCTCCTTATC CCACAAATGC TCCTCCTGCA
    TCATCCCCCT ACTACTCACC AAGCCCCATC AGCGCTCCCC TTCCCATAAT ACCCATGCAG
    AATGTACCTT GCATACTAGG ATCAGGCACA CCTGTATCCA CCCCTCTCAA TGTTCCCACC
    CCTATACCTC TCCTGGCTAT GGCCCTCTCC CCACCCATGC CTCCACCAGA GCAACATCTG
    CAACAGATGT TCTCCCCAAT TCCAACACCA GAAGGTGCCC TTTACCAACC TCAACCCCAA
    CCAACACAGC CCTCCCTTCC CCTTCCAAAT CCAGCATCTC TCCCTCAGCA AGACCCGTCT
    ATTCCTGAAC CTTTTAAAGA GGAATACTCT CACCATGAGG AATTGCAGAT CCTGGCTCCA
    TCTCACACTG TTATGTCACA GATTCAGTCT CCTCCACAGG ACAGTAAGTC AGAAACAAAG
    TTTACCAATG TCCAAGCCTT TTCTGAGACA GTGGAGCATC CTCTGCAGTC TACTGGCATT
    CAGAGAGCTT ATGCTGTTCC TGAGAGTACT GGCACAGCTA GTGCAACTCA GCAAAGGGCA
    GAAACTTCTG CAATGCCCAA ACTCCCAGCA CCAGAGCCCT GCCCATTTCA ACACAGCACT
    GAGGCACCTG CATCTATTCC TCTCCATGCT TTTGTGTGCG ACAGCCTAAA TCAAGATGCA
    GGATCTAGTA AAGAAATGAG TGACAGCTAT GAAGGATCCA CCGGTGGAGG AAAAGGTGAT
    GGCAAACCCA GAAAGCATCA CCGCAAATCT GCTCGCACAC GTTCACGCCA AGAAAAAATT
    AACAAGCCAA AACTAAGCAT GTTAAATGTT AGTAATACGG GTGACAAGAT GGTGGAATGC
    CAGTTGGAGA CACACAATCA TAAAATGGTG ACTTTCAAGT TCGACCTAGA TGGAGATGCG
    CCTGAAGAAA TAGCCACTTA TATGGTGGAA AATGGTTTCA TTCTACCAAT AGAAAAGGAG
    ATTTTCATAG ATCAACTAAA AGACATTGTT GACAAGGCAG AGGATATTTT AAGTGAGGAC
    ATGGATGGAG AGAAGACATC AGAACTTGGA AAAGGACAGA CACCTGGAGA TAAAGGAAGA
    GAGGCAATAA ATGGACAGCA ACCTGGGATT CCCCAGGCAG TTTATCAGCA AAATGTTCTG
    CACACAGGCA AGAGGTGGTT CATCATCTGC CCTGTAGAAG AAGCTCCTGC AGCAAGTCAG
    GATGGATGCT CAGATGGAGG TCCATCTACA CAATCTCCAT CTACAACAAC AATAACTGAA
    GGAACAGCCA CTGCTCAGCC TGTAGAGAGT GCATCAGTAC AACCACCAGA ACCCAGCACA
    GGATCTGCTT CCGCTTCTAT GGATTCAGAA ACCTGTGCCA CAGCGCCTCC ATCTGGAGGA
    AGTGATCCCT ATGGGGTCTG GAGCCCTCTC TCTTTGACAA ATACTGATCC TCTTTCTCTG
    GCTGCTCTTT CCCAAGCTCC TCCTGCCCCG CAAGCAGCTA TGATGCCAGC ACAGCCTGCT
    TCACATTCAG AGGAGGGCCA AAATTTGGGC TCTACCCAGG TCAGTGTACA GCAGTCCCAG
    CCCTGCATAG ATCCTCAAAG CTCTCTTTTT GTGGAAGAGA CTCAAAGTGG CAGATTAGAC
    TCGGTCTCCG CAATGCACAC CGCTCAGCAA ATGGCTGAAA TTGCATGTGC TGCCTCATTG
    GTGGAGGACG TGCCCTGCTG TCCCTTGGTC ATGCCGTTGT CCCTTGAAGT AAGCAGCGGG
    GCTCAGAAGC CATCAACCGT AATTCCGCCA CCATCACAAG ACAGTACATC TGCTCGCGAA
    CAACTCCAAT CCATTACATC TAATCGAGCG GAGCGTGGCC TGCAACCTGT GGTGCTGCAG
    CAGCCTGTTT CCACTGTAAG TGGATCTAAG GCTCCTTCCC TGCCTCAGAG CCCTGCTCCT
    TCACAGCACC ACTTTGTCCC TGGTGAGTCA GATGGAGAGG GGCGTAGCAG AGGAGGGTTT
    GTAGACAGCA CTATCAAGAC TCTGGATGAG AAATTGAGGA ATTTATTGTA CCAAGAGTAC
    GCTCCGATGT ACCCATCGGG AAGTGCAGCT GAAACTCCAG GATCTGGTAC AGAGTATATC
    CAGTCTCCAC CAGGACCTGA GAGTGCCATG GGAGGGTCAG GAACCAGCAC ACCTAGTCTT
    ATAGGAGAGG GACGATTCAG GGCAGGTGAA CAGCTGCCAC AGATCCCAGA GCGGGTAGAC
    AGTTTAAGCG CTCTCAGCGA CTCTGCAGTT GGAGTTACTG TGTCAAGGAG ACATGTGATG
    CCACACTCTA CATCTTGTTC TGGATCTAGA AATCGATATA AGATGGTGCC AAGCTCCACT
    GACATCTTGA CAGGTCAAGG TCGAAAGCAG CGCAGCCTGA GCAGCACGGC ATCTCCAGCA
    CACCCTGGTG GATTTTTGGG AGAATGTACC ATGTACGAAG AGCCGACTAT GTCAGCTACT
    ACAGTTGGCA GGTTCTCAGT GGTCAGCACA GAAGACGAGG TCACGCGTAG AAAGTACAGT
    AGCAGATATT CAGCTCCACC TGACTTTTAT CTGGACGCTC CACCTCCACT GACCAAACGG
    GGGTCTTTGC CAAGGGCGCA GACCTCGACT TCAGCAGATG CCACAGTTTG CACACGATTT
    ATGTCATCTG ACTCTGGGGC AGAAAGCAGC CCTGCAAAAA TGGCACCTAC AACCCCATCC
    CGGCATGGCA GGTCTGAAAG GAGAGGAAGC GACCTCATGA AAAGGGCAGT GGCTTTTCTA
    AAGCGCTCTG GCCGCAGCAG TAGTGTGCAA AGCTCTGATT CACCAAGCCG GAAAGGAGGG
    GTGTATGGAT CCTATGTCAG CAGTGACAAT GACTCAGAGA TGGAGGACTC AGATATTAAA
    AAGGAGCTTC AAAATCTAAG AGAAAAGCAT ATGAAGGAGA TAAGTGAATT GGAGGCCCAC
    CACAGAGAAG AGATTGAGCT TCTCTATGTC AGATTAGGAA AACCACCACC TCCAGGTCTA
    TACATCCCAC CCACAGTGCC CCCTGCTGGA CGCAAGCGCA GGACCAGCAG GCACAAACTA
    AAAGCTGGCA AACTGCTCAG CCCTCTAGTA CAACAACTGA GAAATGTTGC TTCTAAAACT
    GGTGACTCCA GCAAACCTAA TGATTTAACC AAATCTGCTG AGGCGGTCCC GAGCTTGAAT
    GGATCTCCTG TCAGAGCATC CAACTTGTCT GATGGCAGGC CTTATTCTGG GACCGGGACT
    CTGCCTTGTT CTGTTTCTGA GCCAGTTCAG ACCCAACAGC CCTGCTCTCT TAAAGGATCT
    CTGTCTTCCG ACAATATCTA CTATGGTGCA CAGGGAGAAG GACCCGTGCC ACGCAGCCAG
    CCTGCCCAAG GCTGGCCTCC TTCTCCCCAG GCGTCTGCGC AGGTCACCTA TAAATCCAGT
    AGTAAACCAC GCGCTAGATT CCTCAGTGGG CCAGTTTCTC TGTCCATCTG GTCAACACTT
    AAGCGCCTGT GTCTGGGTAA AGAACGTGGC AGCAGATCTG GAGCATCATC AGGTGCCTCA
    AATCAATCCC CTATGCCTCC TGGATCCACT CCTCCTCCTC ACCAGCCTGT TGGCCTTGCC
    CAAGCACAGA CAAACAACAG CAACAACAAA ACAGATGCTT TCACTCAGCA GCTTCACCGA
    CTGGTGGATA ACTGGGCAGA AGGTGTGAGG GCTCCTTCAC GCTCACGCTC CCTCAGTCTA
    AGACCACAGC AACTGACCAG AACCAGGATC TGGAGTGCAA CAGAGGTTCC AGGGTCAATG
    GAAAGATTGA ATGCTTCCCG GCTGCCTCTT TCATGGCCAC AGATTGATTT CCATGCCTCT
    GTGATGGTGA CTGACACTTC ACAAGTGCTC CAGCACAGTT TTATGGTGTC CAGTAACCCT
    TATGAAAAGA TGCTTAACTC CACGCACTTT GACAAGGACC TTTGGCCAGC AATGACTGCC
    AATCTTAACC AAGAAGTCTT TGCTTTCTCT GCAGCGGACT CTCCGTCCTG GACAGCACCT
    TCTTCCCCCA GCGAGGGTCC TAGTTCTAGA AATAGGACCA TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335561.1
    CDS691..7395
    Translation

    Target ORF information:

    RefSeq Version XM_021479886.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGACTTTA TCAACTCTCC AGCCACTAAT GCAGGCATCC
    ACAGATGGTC AGTCTATTCC ACAGCATCTA TTACAGTCAG CATCTTCAAT GCTCAGTACC
    CTACCTCCAC AACCACAACT AAATATTCAG ACACCCCTTC TCCAGCCTCT ACAAATTTCC
    ACAAAGTTTC CTTCGACATA TCCTCTTTTA CCAGAAGGGG GCACATCTAC AGGGACAGAA
    CCTATATCTT TCTCCTCAGT CTCTTACTCT TCTCCTTATC CCACAAATGC TCCTCCTGCA
    TCATCCCCCT ACTACTCACC AAGCCCCATC AGCGCTCCCC TTCCCATAAT ACCCATGCAG
    AATGTACCTT GCATACTAGG ATCAGGCACA CCTGTATCCA CCCCTCTCAA TGTTCCCACC
    CCTATACCTC TCCTGGCTAT GGCCCTCTCC CCACCCATGC CTCCACCAGA GCAACATCTG
    CAACAGATGT TCTCCCCAAT TCCAACACCA GAAGGTGCCC TTTACCAACC TCAACCCCAA
    CCAACACAGC CCTCCCTTCC CCTTCCAAAT CCAGCATCTC TCCCTCAGCA AGACCCGTCT
    ATTCCTGAAC CTTTTAAAGA GGAATACTCT CACCATGAGG AATTGCAGAT CCTGGCTCCA
    TCTCACACTG TTATGTCACA GATTCAGTCT CCTCCACAGG ACAGTAAGTC AGAAACAAAG
    TTTACCAATG TCCAAGCCTT TTCTGAGACA GTGGAGCATC CTCTGCAGTC TACTGGCATT
    CAGAGAGCTT ATGCTGTTCC TGAGAGTACT GGCACAGCTA GTGCAACTCA GCAAAGGGCA
    GAAACTTCTG CAATGCCCAA ACTCCCAGCA CCAGAGCCCT GCCCATTTCA ACACAGCACT
    GAGGCACCTG CATCTATTCC TCTCCATGCT TTTGTGTGCG ACAGCCTAAA TCAAGATGCA
    GGATCTAGTA AAGAAATGAG TGACAGCTAT GAAGGATCCA CCGGTGGAGG AAAAGGTGAT
    GGCAAACCCA GAAAGCATCA CCGCAAATCT GCTCGCACAC GTTCACGCCA AGAAAAAATT
    AACAAGCCAA AACTAAGCAT GTTAAATGTT AGTAATACGG GTGACAAGAT GGTGGAATGC
    CAGTTGGAGA CACACAATCA TAAAATGGTG ACTTTCAAGT TCGACCTAGA TGGAGATGCG
    CCTGAAGAAA TAGCCACTTA TATGGTGGAA AATGGTTTCA TTCTACCAAT AGAAAAGGAG
    ATTTTCATAG ATCAACTAAA AGACATTGTT GACAAGGCAG AGGATATTTT AAGTGAGGAC
    ATGGATGGAG AGAAGACATC AGAACTTGGA AAAGGACAGA CACCTGGAGA TAAAGGAAGA
    GAGGCAATAA ATGGACAGCA ACCTGGGATT CCCCAGGCAG TTTATCAGCA AAATGTTCTG
    CACACAGGCA AGAGGTGGTT CATCATCTGC CCTGTAGAAG AAGCTCCTGC AGCAAGTCAG
    GATGGATGCT CAGATGGAGG TCCATCTACA CAATCTCCAT CTACAACAAC AATAACTGAA
    GGAACAGCCA CTGCTCAGCC TGTAGAGAGT GCATCAGTAC AACCACCAGA ACCCAGCACA
    GGATCTGCTT CCGCTTCTAT GGATTCAGAA ACCTGTGCCA CAGCGCCTCC ATCTGGAGGA
    AGTGATCCCT ATGGGGTCTG GAGCCCTCTC TCTTTGACAA ATACTGATCC TCTTTCTCTG
    GCTGCTCTTT CCCAAGCTCC TCCTGCCCCG CAAGCAGCTA TGATGCCAGC ACAGCCTGCT
    TCACATTCAG AGGAGGGCCA AAATTTGGGC TCTACCCAGG TCAGTGTACA GCAGTCCCAG
    CCCTGCATAG ATCCTCAAAG CTCTCTTTTT GTGGAAGAGA CTCAAAGTGG CAGATTAGAC
    TCGGTCTCCG CAATGCACAC CGCTCAGCAA ATGGCTGAAA TTGCATGTGC TGCCTCATTG
    GTGGAGGACG TGCCCTGCTG TCCCTTGGTC ATGCCGTTGT CCCTTGAAGT AAGCAGCGGG
    GCTCAGAAGC CATCAACCGT AATTCCGCCA CCATCACAAG ACAGTACATC TGCTCGCGAA
    CAACTCCAAT CCATTACATC TAATCGAGCG GAGCGTGGCC TGCAACCTGT GGTGCTGCAG
    CAGCCTGTTT CCACTGTAAG TGGATCTAAG GCTCCTTCCC TGCCTCAGAG CCCTGCTCCT
    TCACAGCACC ACTTTGTCCC TGGTGAGTCA GATGGAGAGG GGCGTAGCAG AGGAGGGTTT
    GTAGACAGCA CTATCAAGAC TCTGGATGAG AAATTGAGGA ATTTATTGTA CCAAGAGTAC
    GCTCCGATGT ACCCATCGGG AAGTGCAGCT GAAACTCCAG GATCTGGTAC AGAGTATATC
    CAGTCTCCAC CAGGACCTGA GAGTGCCATG GGAGGGTCAG GAACCAGCAC ACCTAGTCTT
    ATAGGAGAGG GACGATTCAG GGCAGGTGAA CAGCTGCCAC AGATCCCAGA GCGGGTAGAC
    AGTTTAAGCG CTCTCAGCGA CTCTGCAGTT GGAGTTACTG TGTCAAGGAG ACATGTGATG
    CCACACTCTA CATCTTGTTC TGGATCTAGA AATCGATATA AGATGGTGCC AAGCTCCACT
    GACATCTTGA CAGGTCAAGG TCGAAAGCAG CGCAGCCTGA GCAGCACGGC ATCTCCAGCA
    CACCCTGGTG GATTTTTGGG AGAATGTACC ATGTACGAAG AGCCGACTAT GTCAGCTACT
    ACAGTTGGCA GGTTCTCAGT GGTCAGCACA GAAGACGAGG TCACGCGTAG AAAGTACAGT
    AGCAGATATT CAGCTCCACC TGACTTTTAT CTGGACGCTC CACCTCCACT GACCAAACGG
    GGGTCTTTGC CAAGGGCGCA GACCTCGACT TCAGCAGATG CCACAGTTTG CACACGATTT
    ATGTCATCTG ACTCTGGGGC AGAAAGCAGC CCTGCAAAAA TGGCACCTAC AACCCCATCC
    CGGCATGGCA GGTCTGAAAG GAGAGGAAGC GACCTCATGA AAAGGGCAGT GGCTTTTCTA
    AAGCGCTCTG GCCGCAGCAG TAGTGTGCAA AGCTCTGATT CACCAAGCCG GAAAGGAGGG
    GTGTATGGAT CCTATGTCAG CAGTGACAAT GACTCAGAGA TGGAGGACTC AGATATTAAA
    AAGGAGCTTC AAAATCTAAG AGAAAAGCAT ATGAAGGAGA TAAGTGAATT GGAGGCCCAC
    CACAGAGAAG AGATTGAGCT TCTCTATGTC AGATTAGGAA AACCACCACC TCCAGGTCTA
    TACATCCCAC CCACAGTGCC CCCTGCTGGA CGCAAGCGCA GGACCAGCAG GCACAAACTA
    AAAGCTGGCA AACTGCTCAG CCCTCTAGTA CAACAACTGA GAAATGTTGC TTCTAAAACT
    GGTGACTCCA GCAAACCTAA TGATTTAACC AAATCTGCTG AGGCGGTCCC GAGCTTGAAT
    GGATCTCCTG TCAGAGCATC CAACTTGTCT GATGGCAGGC CTTATTCTGG GACCGGGACT
    CTGCCTTGTT CTGTTTCTGA GCCAGTTCAG ACCCAACAGC CCTGCTCTCT TAAAGGATCT
    CTGTCTTCCG ACAATATCTA CTATGGTGCA CAGGGAGAAG GACCCGTGCC ACGCAGCCAG
    CCTGCCCAAG GCTGGCCTCC TTCTCCCCAG GCGTCTGCGC AGGTCACCTA TAAATCCAGT
    AGTAAACCAC GCGCTAGATT CCTCAGTGGG CCAGTTTCTC TGTCCATCTG GTCAACACTT
    AAGCGCCTGT GTCTGGGTAA AGAACGTGGC AGCAGATCTG GAGCATCATC AGGTGCCTCA
    AATCAATCCC CTATGCCTCC TGGATCCACT CCTCCTCCTC ACCAGCCTGT TGGCCTTGCC
    CAAGCACAGA CAAACAACAG CAACAACAAA ACAGATGCTT TCACTCAGCA GCTTCACCGA
    CTGGTGGATA ACTGGGCAGA AGGTGTGAGG GCTCCTTCAC GCTCACGCTC CCTCAGTCTA
    AGACCACAGC AACTGACCAG AACCAGGATC TGGAGTGCAA CAGAGGTTCC AGGGTCAATG
    GAAAGATTGA ATGCTTCCCG GCTGCCTCTT TCATGGCCAC AGATTGATTT CCATGCCTCT
    GTGATGGTGA CTGACACTTC ACAAGTGCTC CAGCACAGTT TTATGGTGTC CAGTAACCCT
    TATGAAAAGA TGCTTAACTC CACGCACTTT GACAAGGACC TTTGGCCAGC AATGACTGCC
    AATCTTAACC AAGAAGTCTT TGCTTTCTCT GCAGCGGACT CTCCGTCCTG GACAGCACCT
    TCTTCCCCCA GCGAGGGTCC TAGTTCTAGA AATAGGACCA TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148045
    Clone ID Related Accession (Same CDS sequence) XM_021479887.1
    Accession Version XM_021479887.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGTTTCAT CTCTATTTCC ATCCTGAAGC TTTCCTGCCT
    CAGACTTTAT CAACTCTCCA GCCACTAATG CAGGCATCCA CAGATGGTCA GTCTATTCCA
    CAGCATCTAT TACAGTCAGC ATCTTCAATG CTCAGTACCC TACCTCCACA ACCACAACTA
    AATATTCAGA CACCCCTTCT CCAGCCTCTA CAAATTTCCA CAAAGTTTCC TTCGACATAT
    CCTCTTTTAC CAGAAGGGGG CACATCTACA GGGACAGAAC CTATATCTTT CTCCTCAGTC
    TCTTACTCTT CTCCTTATCC CACAAATGCT CCTCCTGCAT CATCCCCCTA CTACTCACCA
    AGCCCCATCA GCGCTCCCCT TCCCATAATA CCCATGCAGA ATGTACCTTG CATACTAGGA
    TCAGGCACAC CTGTATCCAC CCCTCTCAAT GTTCCCACCC CTATACCTCT CCTGGCTATG
    GCCCTCTCCC CACCCATGCC TCCACCAGAG CAACATCTGC AACAGATGTT CTCCCCAATT
    CCAACACCAG AAGGTGCCCT TTACCAACCT CAACCCCAAC CAACACAGCC CTCCCTTCCC
    CTTCCAAATC CAGCATCTCT CCCTCAGCAA GACCCGTCTA TTCCTGAACC TTTTAAAGAG
    GAATACTCTC ACCATGAGGA ATTGCAGATC CTGGCTCCAT CTCACACTGT TATGTCACAG
    ATTCAGTCTC CTCCACAGGA CAGTAAGTCA GAAACAAAGT TTACCAATGT CCAAGCCTTT
    TCTGAGACAG TGGAGCATCC TCTGCAGTCT ACTGGCATTC AGAGAGCTTA TGCTGTTCCT
    GAGAGTACTG GCACAGCTAG TGCAACTCAG CAAAGGGCAG AAACTTCTGC AATGCCCAAA
    CTCCCAGCAC CAGAGCCCTG CCCATTTCAA CACAGCACTG AGGCACCTGC ATCTATTCCT
    CTCCATGCTT TTGTGTGCGA CAGCCTAAAT CAAGATGCAG GATCTAGTAA AGAAATGAGT
    GACAGCTATG AAGGATCCAC CGGTGGAGGA AAAGGTGATG GCAAACCCAG AAAGCATCAC
    CGCAAATCTG CTCGCACACG TTCACGCCAA GAAAAAATTA ACAAGCCAAA ACTAAGCATG
    TTAAATGTTA GTAATACGGG TGACAAGATG GTGGAATGCC AGTTGGAGAC ACACAATCAT
    AAAATGGTGA CTTTCAAGTT CGACCTAGAT GGAGATGCGC CTGAAGAAAT AGCCACTTAT
    ATGGTGGAAA ATGGTTTCAT TCTACCAATA GAAAAGGAGA TTTTCATAGA TCAACTAAAA
    GACATTGTTG ACAAGGCAGA GGATATTTTA AGTGAGGACA TGGATGGAGA GAAGACATCA
    GAACTTGGAA AAGGACAGAC ACCTGGAGAT AAAGGAAGAG AGGCAATAAA TGGACAGCAA
    CCTGGGATTC CCCAGGCAGT TTATCAGCAA AATGTTCTGC ACACAGGCAA GAGGTGGTTC
    ATCATCTGCC CTGTAGAAGA AGCTCCTGCA GCAAGTCAGG ATGGATGCTC AGATGGAGGT
    CCATCTACAC AATCTCCATC TACAACAACA ATAACTGAAG GAACAGCCAC TGCTCAGCCT
    GTAGAGAGTG CATCAGTACA ACCACCAGAA CCCAGCACAG GATCTGCTTC CGCTTCTATG
    GATTCAGAAA CCTGTGCCAC AGCGCCTCCA TCTGGAGGAA GTGATCCCTA TGGGGTCTGG
    AGCCCTCTCT CTTTGACAAA TACTGATCCT CTTTCTCTGG CTGCTCTTTC CCAAGCTCCT
    CCTGCCCCGC AAGCAGCTAT GATGCCAGCA CAGCCTGCTT CACATTCAGA GGAGGGCCAA
    AATTTGGGCT CTACCCAGGT CAGTGTACAG CAGTCCCAGC CCTGCATAGA TCCTCAAAGC
    TCTCTTTTTG TGGAAGAGAC TCAAAGTGGC AGATTAGACT CGGTCTCCGC AATGCACACC
    GCTCAGCAAA TGGCTGAAAT TGCATGTGCT GCCTCATTGG TGGAGGACGT GCCCTGCTGT
    CCCTTGGTCA TGCCGTTGTC CCTTGAAGTA AGCAGCGGGG CTCAGAAGCC ATCAACCGTA
    ATTCCGCCAC CATCACAAGA CAGTACATCT GCTCGCGAAC AACTCCAATC CATTACATCT
    AATCGAGCGG AGCGTGGCCT GCAACCTGTG GTGCTGCAGC AGCCTGTTTC CACTGTAAGT
    GGATCTAAGG CTCCTTCCCT GCCTCAGAGC CCTGCTCCTT CACAGCACCA CTTTGTCCCT
    GGTGAGTCAG ATGGAGAGGG GCGTAGCAGA GGAGGGTTTG TAGACAGCAC TATCAAGACT
    CTGGATGAGA AATTGAGGAA TTTATTGTAC CAAGAGTACG CTCCGATGTA CCCATCGGGA
    AGTGCAGCTG AAACTCCAGG ATCTGGTACA GAGTATATCC AGTCTCCACC AGGACCTGAG
    AGTGCCATGG GAGGGTCAGG AACCAGCACA CCTAGTCTTA TAGGAGAGGG ACGATTCAGG
    GCAGGTGAAC AGCTGCCACA GATCCCAGAG CGGGTAGACA GTTTAAGCGC TCTCAGCGAC
    TCTGCAGTTG GAGTTACTGT GTCAAGGAGA CATGTGATGC CACACTCTAC ATCTTGTTCT
    GGATCTAGAA ATCGATATAA GATGGTGCCA AGCTCCACTG ACATCTTGAC AGGTCAAGGT
    CGAAAGCAGC GCAGCCTGAG CAGCACGGCA TCTCCAGCAC ACCCTGGTGG ATTTTTGGGA
    GAATGTACCA TGTACGAAGA GCCGACTATG TCAGCTACTA CAGTTGGCAG GTTCTCAGTG
    GTCAGCACAG AAGACGAGGT CACGCGTAGA AAGTACAGTA GCAGATATTC AGCTCCACCT
    GACTTTTATC TGGACGCTCC ACCTCCACTG ACCAAACGGG GGTCTTTGCC AAGGGCGCAG
    ACCTCGACTT CAGCAGATGC CACAGTTTGC ACACGATTTA TGTCATCTGA CTCTGGGGCA
    GAAAGCAGCC CTGCAAAAAT GGCACCTACA ACCCCATCCC GGCATGGCAG GTCTGAAAGG
    AGAGGAAGCG ACCTCATGAA AAGGGCAGTG GCTTTTCTAA AGCGCTCTGG CCGCAGCAGT
    AGTGTGCAAA GCTCTGATTC ACCAAGCCGG AAAGGAGGGG TGTATGGATC CTATGTCAGC
    AGTGACAATG ACTCAGAGAT GGAGGACTCA GATATTAAAA AGGAGCTTCA AAATCTAAGA
    GAAAAGCATA TGAAGGAGAT AAGTGAATTG GAGGCCCACC ACAGAGAAGA GATTGAGCTT
    CTCTATGTCA GATTAGGAAA ACCACCACCT CCAGGTCTAT ACATCCCACC CACAGTGCCC
    CCTGCTGGAC GCAAGCGCAG GACCAGCAGG CACAAACTAA AAGCTGGCAA ACTGCTCAGC
    CCTCTAGTAC AACAACTGAG AAATGTTGCT TCTAAAACTG GTGACTCCAG CAAACCTAAT
    GATTTAACCA AATCTGCTGA GGCGGTCCCG AGCTTGAATG GATCTCCTGT CAGAGCATCC
    AACTTGTCTG ATGGCAGGCC TTATTCTGGG ACCGGGACTC TGCCTTGTTC TGTTTCTGAG
    CCAGTTCAGA CCCAACAGCC CTGCTCTCTT AAAGGATCTC TGTCTTCCGA CAATATCTAC
    TATGGTGCAC AGGGAGAAGG ACCCGTGCCA CGCAGCCAGC CTGCCCAAGG CTGGCCTCCT
    TCTCCCCAGG CGTCTGCGCA GGTCACCTAT AAATCCAGTA GTAAACCACG CGCTAGATTC
    CTCAGTGGGC CAGTTTCTCT GTCCATCTGG TCAACACTTA AGCGCCTGTG TCTGGGTAAA
    GAACGTGGCA GCAGATCTGG AGCATCATCA GGTGCCTCAA ATCAATCCCC TATGCCTCCT
    GGATCCACTC CTCCTCCTCA CCAGCCTGTT GGCCTTGCCC AAGCACAGAC AAACAACAGC
    AACAACAAAA CAGATGCTTT CACTCAGCAG CTTCACCGAC TGGTGGATAA CTGGGCAGAA
    GGTGTGAGGG CTCCTTCACG CTCACGCTCC CTCAGTCTAA GACCACAGCA ACTGACCAGA
    ACCAGGATCT GGAGTGCAAC AGAGAATGTC TATCAATCAA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335562.1
    CDS698..7162
    Translation

    Target ORF information:

    RefSeq Version XM_021479887.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479887.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGTTTCAT CTCTATTTCC ATCCTGAAGC TTTCCTGCCT
    CAGACTTTAT CAACTCTCCA GCCACTAATG CAGGCATCCA CAGATGGTCA GTCTATTCCA
    CAGCATCTAT TACAGTCAGC ATCTTCAATG CTCAGTACCC TACCTCCACA ACCACAACTA
    AATATTCAGA CACCCCTTCT CCAGCCTCTA CAAATTTCCA CAAAGTTTCC TTCGACATAT
    CCTCTTTTAC CAGAAGGGGG CACATCTACA GGGACAGAAC CTATATCTTT CTCCTCAGTC
    TCTTACTCTT CTCCTTATCC CACAAATGCT CCTCCTGCAT CATCCCCCTA CTACTCACCA
    AGCCCCATCA GCGCTCCCCT TCCCATAATA CCCATGCAGA ATGTACCTTG CATACTAGGA
    TCAGGCACAC CTGTATCCAC CCCTCTCAAT GTTCCCACCC CTATACCTCT CCTGGCTATG
    GCCCTCTCCC CACCCATGCC TCCACCAGAG CAACATCTGC AACAGATGTT CTCCCCAATT
    CCAACACCAG AAGGTGCCCT TTACCAACCT CAACCCCAAC CAACACAGCC CTCCCTTCCC
    CTTCCAAATC CAGCATCTCT CCCTCAGCAA GACCCGTCTA TTCCTGAACC TTTTAAAGAG
    GAATACTCTC ACCATGAGGA ATTGCAGATC CTGGCTCCAT CTCACACTGT TATGTCACAG
    ATTCAGTCTC CTCCACAGGA CAGTAAGTCA GAAACAAAGT TTACCAATGT CCAAGCCTTT
    TCTGAGACAG TGGAGCATCC TCTGCAGTCT ACTGGCATTC AGAGAGCTTA TGCTGTTCCT
    GAGAGTACTG GCACAGCTAG TGCAACTCAG CAAAGGGCAG AAACTTCTGC AATGCCCAAA
    CTCCCAGCAC CAGAGCCCTG CCCATTTCAA CACAGCACTG AGGCACCTGC ATCTATTCCT
    CTCCATGCTT TTGTGTGCGA CAGCCTAAAT CAAGATGCAG GATCTAGTAA AGAAATGAGT
    GACAGCTATG AAGGATCCAC CGGTGGAGGA AAAGGTGATG GCAAACCCAG AAAGCATCAC
    CGCAAATCTG CTCGCACACG TTCACGCCAA GAAAAAATTA ACAAGCCAAA ACTAAGCATG
    TTAAATGTTA GTAATACGGG TGACAAGATG GTGGAATGCC AGTTGGAGAC ACACAATCAT
    AAAATGGTGA CTTTCAAGTT CGACCTAGAT GGAGATGCGC CTGAAGAAAT AGCCACTTAT
    ATGGTGGAAA ATGGTTTCAT TCTACCAATA GAAAAGGAGA TTTTCATAGA TCAACTAAAA
    GACATTGTTG ACAAGGCAGA GGATATTTTA AGTGAGGACA TGGATGGAGA GAAGACATCA
    GAACTTGGAA AAGGACAGAC ACCTGGAGAT AAAGGAAGAG AGGCAATAAA TGGACAGCAA
    CCTGGGATTC CCCAGGCAGT TTATCAGCAA AATGTTCTGC ACACAGGCAA GAGGTGGTTC
    ATCATCTGCC CTGTAGAAGA AGCTCCTGCA GCAAGTCAGG ATGGATGCTC AGATGGAGGT
    CCATCTACAC AATCTCCATC TACAACAACA ATAACTGAAG GAACAGCCAC TGCTCAGCCT
    GTAGAGAGTG CATCAGTACA ACCACCAGAA CCCAGCACAG GATCTGCTTC CGCTTCTATG
    GATTCAGAAA CCTGTGCCAC AGCGCCTCCA TCTGGAGGAA GTGATCCCTA TGGGGTCTGG
    AGCCCTCTCT CTTTGACAAA TACTGATCCT CTTTCTCTGG CTGCTCTTTC CCAAGCTCCT
    CCTGCCCCGC AAGCAGCTAT GATGCCAGCA CAGCCTGCTT CACATTCAGA GGAGGGCCAA
    AATTTGGGCT CTACCCAGGT CAGTGTACAG CAGTCCCAGC CCTGCATAGA TCCTCAAAGC
    TCTCTTTTTG TGGAAGAGAC TCAAAGTGGC AGATTAGACT CGGTCTCCGC AATGCACACC
    GCTCAGCAAA TGGCTGAAAT TGCATGTGCT GCCTCATTGG TGGAGGACGT GCCCTGCTGT
    CCCTTGGTCA TGCCGTTGTC CCTTGAAGTA AGCAGCGGGG CTCAGAAGCC ATCAACCGTA
    ATTCCGCCAC CATCACAAGA CAGTACATCT GCTCGCGAAC AACTCCAATC CATTACATCT
    AATCGAGCGG AGCGTGGCCT GCAACCTGTG GTGCTGCAGC AGCCTGTTTC CACTGTAAGT
    GGATCTAAGG CTCCTTCCCT GCCTCAGAGC CCTGCTCCTT CACAGCACCA CTTTGTCCCT
    GGTGAGTCAG ATGGAGAGGG GCGTAGCAGA GGAGGGTTTG TAGACAGCAC TATCAAGACT
    CTGGATGAGA AATTGAGGAA TTTATTGTAC CAAGAGTACG CTCCGATGTA CCCATCGGGA
    AGTGCAGCTG AAACTCCAGG ATCTGGTACA GAGTATATCC AGTCTCCACC AGGACCTGAG
    AGTGCCATGG GAGGGTCAGG AACCAGCACA CCTAGTCTTA TAGGAGAGGG ACGATTCAGG
    GCAGGTGAAC AGCTGCCACA GATCCCAGAG CGGGTAGACA GTTTAAGCGC TCTCAGCGAC
    TCTGCAGTTG GAGTTACTGT GTCAAGGAGA CATGTGATGC CACACTCTAC ATCTTGTTCT
    GGATCTAGAA ATCGATATAA GATGGTGCCA AGCTCCACTG ACATCTTGAC AGGTCAAGGT
    CGAAAGCAGC GCAGCCTGAG CAGCACGGCA TCTCCAGCAC ACCCTGGTGG ATTTTTGGGA
    GAATGTACCA TGTACGAAGA GCCGACTATG TCAGCTACTA CAGTTGGCAG GTTCTCAGTG
    GTCAGCACAG AAGACGAGGT CACGCGTAGA AAGTACAGTA GCAGATATTC AGCTCCACCT
    GACTTTTATC TGGACGCTCC ACCTCCACTG ACCAAACGGG GGTCTTTGCC AAGGGCGCAG
    ACCTCGACTT CAGCAGATGC CACAGTTTGC ACACGATTTA TGTCATCTGA CTCTGGGGCA
    GAAAGCAGCC CTGCAAAAAT GGCACCTACA ACCCCATCCC GGCATGGCAG GTCTGAAAGG
    AGAGGAAGCG ACCTCATGAA AAGGGCAGTG GCTTTTCTAA AGCGCTCTGG CCGCAGCAGT
    AGTGTGCAAA GCTCTGATTC ACCAAGCCGG AAAGGAGGGG TGTATGGATC CTATGTCAGC
    AGTGACAATG ACTCAGAGAT GGAGGACTCA GATATTAAAA AGGAGCTTCA AAATCTAAGA
    GAAAAGCATA TGAAGGAGAT AAGTGAATTG GAGGCCCACC ACAGAGAAGA GATTGAGCTT
    CTCTATGTCA GATTAGGAAA ACCACCACCT CCAGGTCTAT ACATCCCACC CACAGTGCCC
    CCTGCTGGAC GCAAGCGCAG GACCAGCAGG CACAAACTAA AAGCTGGCAA ACTGCTCAGC
    CCTCTAGTAC AACAACTGAG AAATGTTGCT TCTAAAACTG GTGACTCCAG CAAACCTAAT
    GATTTAACCA AATCTGCTGA GGCGGTCCCG AGCTTGAATG GATCTCCTGT CAGAGCATCC
    AACTTGTCTG ATGGCAGGCC TTATTCTGGG ACCGGGACTC TGCCTTGTTC TGTTTCTGAG
    CCAGTTCAGA CCCAACAGCC CTGCTCTCTT AAAGGATCTC TGTCTTCCGA CAATATCTAC
    TATGGTGCAC AGGGAGAAGG ACCCGTGCCA CGCAGCCAGC CTGCCCAAGG CTGGCCTCCT
    TCTCCCCAGG CGTCTGCGCA GGTCACCTAT AAATCCAGTA GTAAACCACG CGCTAGATTC
    CTCAGTGGGC CAGTTTCTCT GTCCATCTGG TCAACACTTA AGCGCCTGTG TCTGGGTAAA
    GAACGTGGCA GCAGATCTGG AGCATCATCA GGTGCCTCAA ATCAATCCCC TATGCCTCCT
    GGATCCACTC CTCCTCCTCA CCAGCCTGTT GGCCTTGCCC AAGCACAGAC AAACAACAGC
    AACAACAAAA CAGATGCTTT CACTCAGCAG CTTCACCGAC TGGTGGATAA CTGGGCAGAA
    GGTGTGAGGG CTCCTTCACG CTCACGCTCC CTCAGTCTAA GACCACAGCA ACTGACCAGA
    ACCAGGATCT GGAGTGCAAC AGAGAATGTC TATCAATCAA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148046
    Clone ID Related Accession (Same CDS sequence) XM_021479888.1
    Accession Version XM_021479888.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6309bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGTTTCAT CTCTATTTCC ATCCTGAAGC TTTCCTGCCT
    CAGACTTTAT CAACTCTCCA GCCACTAATG CAGGCATCCA CAGATGGTCA GTCTATTCCA
    CAGCATCTAT TACAGTCAGC ATCTTCAATG CTCAGTACCC TACCTCCACA ACCACAACTA
    AATATTCAGA CACCCCTTCT CCAGCCTCTA CAAATTTCCA CAAAGGAATA CTCTCACCAT
    GAGGAATTGC AGATCCTGGC TCCATCTCAC ACTGTTATGT CACAGATTCA GTCTCCTCCA
    CAGGACAGTA AGTCAGAAAC AAAGTTTACC AATGTCCAAG CCTTTTCTGA GACAGTGGAG
    CATCCTCTGC AGTCTACTGG CATTCAGAGA GCTTATGCTG TTCCTGAGAG TACTGGCACA
    GCTAGTGCAA CTCAGCAAAG GGCAGAAACT TCTGCAATGC CCAAACTCCC AGCACCAGAG
    CCCTGCCCAT TTCAACACAG CACTGAGGCA CCTGCATCTA TTCCTCTCCA TGCTTTTGTG
    TGCGACAGCC TAAATCAAGA TGCAGGATCT AGTAAAGAAA TGAGTGACAG CTATGAAGGA
    TCCACCGGTG GAGGAAAAGG TGATGGCAAA CCCAGAAAGC ATCACCGCAA ATCTGCTCGC
    ACACGTTCAC GCCAAGAAAA AATTAACAAG CCAAAACTAA GCATGTTAAA TGTTAGTAAT
    ACGGGTGACA AGATGGTGGA ATGCCAGTTG GAGACACACA ATCATAAAAT GGTGACTTTC
    AAGTTCGACC TAGATGGAGA TGCGCCTGAA GAAATAGCCA CTTATATGGT GGAAAATGGT
    TTCATTCTAC CAATAGAAAA GGAGATTTTC ATAGATCAAC TAAAAGACAT TGTTGACAAG
    GCAGAGGATA TTTTAAGTGA GGACATGGAT GGAGAGAAGA CATCAGAACT TGGAAAAGGA
    CAGACACCTG GAGATAAAGG AAGAGAGGCA ATAAATGGAC AGCAACCTGG GATTCCCCAG
    GCAGTTTATC AGCAAAATGT TCTGCACACA GGCAAGAGGT GGTTCATCAT CTGCCCTGTA
    GAAGAAGCTC CTGCAGCAAG TCAGGATGGA TGCTCAGATG GAGGTCCATC TACACAATCT
    CCATCTACAA CAACAATAAC TGAAGGAACA GCCACTGCTC AGCCTGTAGA GAGTGCATCA
    GTACAACCAC CAGAACCCAG CACAGGATCT GCTTCCGCTT CTATGGATTC AGAAACCTGT
    GCCACAGCGC CTCCATCTGG AGGAAGTGAT CCCTATGGGG TCTGGAGCCC TCTCTCTTTG
    ACAAATACTG ATCCTCTTTC TCTGGCTGCT CTTTCCCAAG CTCCTCCTGC CCCGCAAGCA
    GCTATGATGC CAGCACAGCC TGCTTCACAT TCAGAGGAGG GCCAAAATTT GGGCTCTACC
    CAGGTCAGTG TACAGCAGTC CCAGCCCTGC ATAGATCCTC AAAGCTCTCT TTTTGTGGAA
    GAGACTCAAA GTGGCAGATT AGACTCGGTC TCCGCAATGC ACACCGCTCA GCAAATGGCT
    GAAATTGCAT GTGCTGCCTC ATTGGTGGAG GACGTGCCCT GCTGTCCCTT GGTCATGCCG
    TTGTCCCTTG AAGTAAGCAG CGGGGCTCAG AAGCCATCAA CCGTAATTCC GCCACCATCA
    CAAGACAGTA CATCTGCTCG CGAACAACTC CAATCCATTA CATCTAATCG AGCGGAGCGT
    GGCCTGCAAC CTGTGGTGCT GCAGCAGCCT GTTTCCACTG TAAGTGGATC TAAGGCTCCT
    TCCCTGCCTC AGAGCCCTGC TCCTTCACAG CACCACTTTG TCCCTGGTGA GTCAGATGGA
    GAGGGGCGTA GCAGAGGAGG GTTTGTAGAC AGCACTATCA AGACTCTGGA TGAGAAATTG
    AGGAATTTAT TGTACCAAGA GTACGCTCCG ATGTACCCAT CGGGAAGTGC AGCTGAAACT
    CCAGGATCTG GTACAGAGTA TATCCAGTCT CCACCAGGAC CTGAGAGTGC CATGGGAGGG
    TCAGGAACCA GCACACCTAG TCTTATAGGA GAGGGACGAT TCAGGGCAGG TGAACAGCTG
    CCACAGATCC CAGAGCGGGT AGACAGTTTA AGCGCTCTCA GCGACTCTGC AGTTGGAGTT
    ACTGTGTCAA GGAGACATGT GATGCCACAC TCTACATCTT GTTCTGGATC TAGAAATCGA
    TATAAGATGG TGCCAAGCTC CACTGACATC TTGACAGGTC AAGGTCGAAA GCAGCGCAGC
    CTGAGCAGCA CGGCATCTCC AGCACACCCT GGTGGATTTT TGGGAGAATG TACCATGTAC
    GAAGAGCCGA CTATGTCAGC TACTACAGTT GGCAGGTTCT CAGTGGTCAG CACAGAAGAC
    GAGGTCACGC GTAGAAAGTA CAGTAGCAGA TATTCAGCTC CACCTGACTT TTATCTGGAC
    GCTCCACCTC CACTGACCAA ACGGGGGTCT TTGCCAAGGG CGCAGACCTC GACTTCAGCA
    GATGCCACAG TTTGCACACG ATTTATGTCA TCTGACTCTG GGGCAGAAAG CAGCCCTGCA
    AAAATGGCAC CTACAACCCC ATCCCGGCAT GGCAGGTCTG AAAGGAGAGG AAGCGACCTC
    ATGAAAAGGG CAGTGGCTTT TCTAAAGCGC TCTGGCCGCA GCAGTAGTGT GCAAAGCTCT
    GATTCACCAA GCCGGAAAGG AGGGGTGTAT GGATCCTATG TCAGCAGTGA CAATGACTCA
    GAGATGGAGG ACTCAGATAT TAAAAAGGAG CTTCAAAATC TAAGAGAAAA GCATATGAAG
    GAGATAAGTG AATTGGAGGC CCACCACAGA GAAGAGATTG AGCTTCTCTA TGTCAGATTA
    GGAAAACCAC CACCTCCAGG TCTATACATC CCACCCACAG TGCCCCCTGC TGGACGCAAG
    CGCAGGACCA GCAGGCACAA ACTAAAAGCT GGCAAACTGC TCAGCCCTCT AGTACAACAA
    CTGAGAAATG TTGCTTCTAA AACTGGTGAC TCCAGCAAAC CTAATGATTT AACCAAATCT
    GCTGAGGCGG TCCCGAGCTT GAATGGATCT CCTGTCAGAG CATCCAACTT GTCTGATGGC
    AGGCCTTATT CTGGGACCGG GACTCTGCCT TGTTCTGTTT CTGAGCCAGT TCAGACCCAA
    CAGCCCTGCT CTCTTAAAGG ATCTCTGTCT TCCGACAATA TCTACTATGG TGCACAGGGA
    GAAGGACCCG TGCCACGCAG CCAGCCTGCC CAAGGCTGGC CTCCTTCTCC CCAGGCGTCT
    GCGCAGGTCA CCTATAAATC CAGTAGTAAA CCACGCGCTA GATTCCTCAG TGGGCCAGTT
    TCTCTGTCCA TCTGGTCAAC ACTTAAGCGC CTGTGTCTGG GTAAAGAACG TGGCAGCAGA
    TCTGGAGCAT CATCAGGTGC CTCAAATCAA TCCCCTATGC CTCCTGGATC CACTCCTCCT
    CCTCACCAGC CTGTTGGCCT TGCCCAAGCA CAGACAAACA ACAGCAACAA CAAAACAGAT
    GCTTTCACTC AGCAGCTTCA CCGACTGGTG GATAACTGGG CAGAAGGTGT GAGGGCTCCT
    TCACGCTCAC GCTCCCTCAG TCTAAGACCA CAGCAACTGA CCAGAACCAG GATCTGGAGT
    GCAACAGAGG TTCCAGGGTC AATGGAAAGA TTGAATGCTT CCCGGCTGCC TCTTTCATGG
    CCACAGATTG ATTTCCATGC CTCTGTGATG GTGACTGACA CTTCACAAGT GCTCCAGCAC
    AGTTTTATGG TGTCCAGTAA CCCTTATGAA AAGATGCTTA ACTCCACGCA CTTTGACAAG
    GACCTTTGGC CAGCAATGAC TGCCAATCTT AACCAAGAAG TCTTTGCTTT CTCTGCAGCG
    GACTCTCCGT CCTGGACAGC ACCTTCTTCC CCCAGCGAGG GTCCTAGTTC TAGAAATAGG
    ACCATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335563.1
    CDS688..6996
    Translation

    Target ORF information:

    RefSeq Version XM_021479888.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479888.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGTTTCAT CTCTATTTCC ATCCTGAAGC TTTCCTGCCT
    CAGACTTTAT CAACTCTCCA GCCACTAATG CAGGCATCCA CAGATGGTCA GTCTATTCCA
    CAGCATCTAT TACAGTCAGC ATCTTCAATG CTCAGTACCC TACCTCCACA ACCACAACTA
    AATATTCAGA CACCCCTTCT CCAGCCTCTA CAAATTTCCA CAAAGGAATA CTCTCACCAT
    GAGGAATTGC AGATCCTGGC TCCATCTCAC ACTGTTATGT CACAGATTCA GTCTCCTCCA
    CAGGACAGTA AGTCAGAAAC AAAGTTTACC AATGTCCAAG CCTTTTCTGA GACAGTGGAG
    CATCCTCTGC AGTCTACTGG CATTCAGAGA GCTTATGCTG TTCCTGAGAG TACTGGCACA
    GCTAGTGCAA CTCAGCAAAG GGCAGAAACT TCTGCAATGC CCAAACTCCC AGCACCAGAG
    CCCTGCCCAT TTCAACACAG CACTGAGGCA CCTGCATCTA TTCCTCTCCA TGCTTTTGTG
    TGCGACAGCC TAAATCAAGA TGCAGGATCT AGTAAAGAAA TGAGTGACAG CTATGAAGGA
    TCCACCGGTG GAGGAAAAGG TGATGGCAAA CCCAGAAAGC ATCACCGCAA ATCTGCTCGC
    ACACGTTCAC GCCAAGAAAA AATTAACAAG CCAAAACTAA GCATGTTAAA TGTTAGTAAT
    ACGGGTGACA AGATGGTGGA ATGCCAGTTG GAGACACACA ATCATAAAAT GGTGACTTTC
    AAGTTCGACC TAGATGGAGA TGCGCCTGAA GAAATAGCCA CTTATATGGT GGAAAATGGT
    TTCATTCTAC CAATAGAAAA GGAGATTTTC ATAGATCAAC TAAAAGACAT TGTTGACAAG
    GCAGAGGATA TTTTAAGTGA GGACATGGAT GGAGAGAAGA CATCAGAACT TGGAAAAGGA
    CAGACACCTG GAGATAAAGG AAGAGAGGCA ATAAATGGAC AGCAACCTGG GATTCCCCAG
    GCAGTTTATC AGCAAAATGT TCTGCACACA GGCAAGAGGT GGTTCATCAT CTGCCCTGTA
    GAAGAAGCTC CTGCAGCAAG TCAGGATGGA TGCTCAGATG GAGGTCCATC TACACAATCT
    CCATCTACAA CAACAATAAC TGAAGGAACA GCCACTGCTC AGCCTGTAGA GAGTGCATCA
    GTACAACCAC CAGAACCCAG CACAGGATCT GCTTCCGCTT CTATGGATTC AGAAACCTGT
    GCCACAGCGC CTCCATCTGG AGGAAGTGAT CCCTATGGGG TCTGGAGCCC TCTCTCTTTG
    ACAAATACTG ATCCTCTTTC TCTGGCTGCT CTTTCCCAAG CTCCTCCTGC CCCGCAAGCA
    GCTATGATGC CAGCACAGCC TGCTTCACAT TCAGAGGAGG GCCAAAATTT GGGCTCTACC
    CAGGTCAGTG TACAGCAGTC CCAGCCCTGC ATAGATCCTC AAAGCTCTCT TTTTGTGGAA
    GAGACTCAAA GTGGCAGATT AGACTCGGTC TCCGCAATGC ACACCGCTCA GCAAATGGCT
    GAAATTGCAT GTGCTGCCTC ATTGGTGGAG GACGTGCCCT GCTGTCCCTT GGTCATGCCG
    TTGTCCCTTG AAGTAAGCAG CGGGGCTCAG AAGCCATCAA CCGTAATTCC GCCACCATCA
    CAAGACAGTA CATCTGCTCG CGAACAACTC CAATCCATTA CATCTAATCG AGCGGAGCGT
    GGCCTGCAAC CTGTGGTGCT GCAGCAGCCT GTTTCCACTG TAAGTGGATC TAAGGCTCCT
    TCCCTGCCTC AGAGCCCTGC TCCTTCACAG CACCACTTTG TCCCTGGTGA GTCAGATGGA
    GAGGGGCGTA GCAGAGGAGG GTTTGTAGAC AGCACTATCA AGACTCTGGA TGAGAAATTG
    AGGAATTTAT TGTACCAAGA GTACGCTCCG ATGTACCCAT CGGGAAGTGC AGCTGAAACT
    CCAGGATCTG GTACAGAGTA TATCCAGTCT CCACCAGGAC CTGAGAGTGC CATGGGAGGG
    TCAGGAACCA GCACACCTAG TCTTATAGGA GAGGGACGAT TCAGGGCAGG TGAACAGCTG
    CCACAGATCC CAGAGCGGGT AGACAGTTTA AGCGCTCTCA GCGACTCTGC AGTTGGAGTT
    ACTGTGTCAA GGAGACATGT GATGCCACAC TCTACATCTT GTTCTGGATC TAGAAATCGA
    TATAAGATGG TGCCAAGCTC CACTGACATC TTGACAGGTC AAGGTCGAAA GCAGCGCAGC
    CTGAGCAGCA CGGCATCTCC AGCACACCCT GGTGGATTTT TGGGAGAATG TACCATGTAC
    GAAGAGCCGA CTATGTCAGC TACTACAGTT GGCAGGTTCT CAGTGGTCAG CACAGAAGAC
    GAGGTCACGC GTAGAAAGTA CAGTAGCAGA TATTCAGCTC CACCTGACTT TTATCTGGAC
    GCTCCACCTC CACTGACCAA ACGGGGGTCT TTGCCAAGGG CGCAGACCTC GACTTCAGCA
    GATGCCACAG TTTGCACACG ATTTATGTCA TCTGACTCTG GGGCAGAAAG CAGCCCTGCA
    AAAATGGCAC CTACAACCCC ATCCCGGCAT GGCAGGTCTG AAAGGAGAGG AAGCGACCTC
    ATGAAAAGGG CAGTGGCTTT TCTAAAGCGC TCTGGCCGCA GCAGTAGTGT GCAAAGCTCT
    GATTCACCAA GCCGGAAAGG AGGGGTGTAT GGATCCTATG TCAGCAGTGA CAATGACTCA
    GAGATGGAGG ACTCAGATAT TAAAAAGGAG CTTCAAAATC TAAGAGAAAA GCATATGAAG
    GAGATAAGTG AATTGGAGGC CCACCACAGA GAAGAGATTG AGCTTCTCTA TGTCAGATTA
    GGAAAACCAC CACCTCCAGG TCTATACATC CCACCCACAG TGCCCCCTGC TGGACGCAAG
    CGCAGGACCA GCAGGCACAA ACTAAAAGCT GGCAAACTGC TCAGCCCTCT AGTACAACAA
    CTGAGAAATG TTGCTTCTAA AACTGGTGAC TCCAGCAAAC CTAATGATTT AACCAAATCT
    GCTGAGGCGG TCCCGAGCTT GAATGGATCT CCTGTCAGAG CATCCAACTT GTCTGATGGC
    AGGCCTTATT CTGGGACCGG GACTCTGCCT TGTTCTGTTT CTGAGCCAGT TCAGACCCAA
    CAGCCCTGCT CTCTTAAAGG ATCTCTGTCT TCCGACAATA TCTACTATGG TGCACAGGGA
    GAAGGACCCG TGCCACGCAG CCAGCCTGCC CAAGGCTGGC CTCCTTCTCC CCAGGCGTCT
    GCGCAGGTCA CCTATAAATC CAGTAGTAAA CCACGCGCTA GATTCCTCAG TGGGCCAGTT
    TCTCTGTCCA TCTGGTCAAC ACTTAAGCGC CTGTGTCTGG GTAAAGAACG TGGCAGCAGA
    TCTGGAGCAT CATCAGGTGC CTCAAATCAA TCCCCTATGC CTCCTGGATC CACTCCTCCT
    CCTCACCAGC CTGTTGGCCT TGCCCAAGCA CAGACAAACA ACAGCAACAA CAAAACAGAT
    GCTTTCACTC AGCAGCTTCA CCGACTGGTG GATAACTGGG CAGAAGGTGT GAGGGCTCCT
    TCACGCTCAC GCTCCCTCAG TCTAAGACCA CAGCAACTGA CCAGAACCAG GATCTGGAGT
    GCAACAGAGG TTCCAGGGTC AATGGAAAGA TTGAATGCTT CCCGGCTGCC TCTTTCATGG
    CCACAGATTG ATTTCCATGC CTCTGTGATG GTGACTGACA CTTCACAAGT GCTCCAGCAC
    AGTTTTATGG TGTCCAGTAA CCCTTATGAA AAGATGCTTA ACTCCACGCA CTTTGACAAG
    GACCTTTGGC CAGCAATGAC TGCCAATCTT AACCAAGAAG TCTTTGCTTT CTCTGCAGCG
    GACTCTCCGT CCTGGACAGC ACCTTCTTCC CCCAGCGAGG GTCCTAGTTC TAGAAATAGG
    ACCATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53084
    Clone ID Related Accession (Same CDS sequence) XM_009306230.2 , XM_009306231.2
    Accession Version XM_009306230.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GTTTCCTTCG ACATATCCTC TTTTACCAGA AGGGGGCACA
    TCTACAGGGA CAGAACCTAT ATCTTTCTCC TCAGTCTCTT ACTCTTCTCC TTATCCCACA
    AATGCTCCTC CTGCATCATC CCCCTACTAC TCACCAAGCC CCATCAGCGC TCCCCTTCCC
    ATAATACCCA TGCAGAATGT ACCTTGCATA CTAGGATCAG GCACACCTGT ATCCACCCCT
    CTCAATGTTC CCACCCCTAT ACCTCTCCTG GCTATGGCCC TCTCCCCACC CATGCCTCCA
    CCAGAGCAAC ATCTGCAACA GATGTTCTCC CCAATTCCAA CACCAGAAGG TGCCCTTTAC
    CAACCTCAAC CCCAACCAAC ACAGCCCTCC CTTCCCCTTC CAAATCCAGC ATCTCTCCCT
    CAGCAAGACC CGTCTATTCC TGAACCTTTT AAAGAGGAAT ACTCTCACCA TGAGGAATTG
    CAGATCCTGG CTCCATCTCA CACTGTTATG TCACAGATTC AGTCTCCTCC ACAGGACAGT
    AAGTCAGAAA CAAAGTTTAC CAATGTCCAA GCCTTTTCTG AGACAGTGGA GCATCCTCTG
    CAGTCTACTG GCATTCAGAG AGCTTATGCT GTTCCTGAGA GTACTGGCAC AGCTAGTGCA
    ACTCAGCAAA GGGCAGAAAC TTCTGCAATG CCCAAACTCC CAGCACCAGA GCCCTGCCCA
    TTTCAACACA GCACTGAGGC ACCTGCATCT ATTCCTCTCC ATGCTTTTGT GTGCGACAGC
    CTAAATCAAG ATGCAGGATC TAGTAAAGAA ATGAGTGACA GCTATGAAGG ATCCACCGGT
    GGAGGAAAAG GTGATGGCAA ACCCAGAAAG CATCACCGCA AATCTGCTCG CACACGTTCA
    CGCCAAGAAA AAATTAACAA GCCAAAACTA AGCATGTTAA ATGTTAGTAA TACGGGTGAC
    AAGATGGTGG AATGCCAGTT GGAGACACAC AATCATAAAA TGGTGACTTT CAAGTTCGAC
    CTAGATGGAG ATGCGCCTGA AGAAATAGCC ACTTATATGG TGGAAAATGG TTTCATTCTA
    CCAATAGAAA AGGAGATTTT CATAGATCAA CTAAAAGACA TTGTTGACAA GGCAGAGGAT
    ATTTTAAGTG AGGACATGGA TGGAGAGAAG ACATCAGAAC TTGGAAAAGG ACAGACACCT
    GGAGATAAAG GAAGAGAGGC AATAAATGGA CAGCAACCTG GGATTCCCCA GGCAGTTTAT
    CAGCAAAATG TTCTGCACAC AGGCAAGAGG TGGTTCATCA TCTGCCCTGT AGAAGAAGCT
    CCTGCAGCAA GTCAGGATGG ATGCTCAGAT GGAGGTCCAT CTACACAATC TCCATCTACA
    ACAACAATAA CTGAAGGAAC AGCCACTGCT CAGCCTGTAG AGAGTGCATC AGTACAACCA
    CCAGAACCCA GCACAGGATC TGCTTCCGCT TCTATGGATT CAGAAACCTG TGCCACAGCG
    CCTCCATCTG GAGGAAGTGA TCCCTATGGG GTCTGGAGCC CTCTCTCTTT GACAAATACT
    GATCCTCTTT CTCTGGCTGC TCTTTCCCAA GCTCCTCCTG CCCCGCAAGC AGCTATGATG
    CCAGCACAGC CTGCTTCACA TTCAGAGGAG GGCCAAAATT TGGGCTCTAC CCAGGTCAGT
    GTACAGCAGT CCCAGCCCTG CATAGATCCT CAAAGCTCTC TTTTTGTGGA AGAGACTCAA
    AGTGGCAGAT TAGACTCGGT CTCCGCAATG CACACCGCTC AGCAAATGGC TGAAATTGCA
    TGTGCTGCCT CATTGGTGGA GGACGTGCCC TGCTGTCCCT TGGTCATGCC GTTGTCCCTT
    GAAGTAAGCA GCGGGGCTCA GAAGCCATCA ACCGTAATTC CGCCACCATC ACAAGACAGT
    ACATCTGCTC GCGAACAACT CCAATCCATT ACATCTAATC GAGCGGAGCG TGGCCTGCAA
    CCTGTGGTGC TGCAGCAGCC TGTTTCCACT GTAAGTGGAT CTAAGGCTCC TTCCCTGCCT
    CAGAGCCCTG CTCCTTCACA GCACCACTTT GTCCCTGGTG AGTCAGATGG AGAGGGGCGT
    AGCAGAGGAG GGTTTGTAGA CAGCACTATC AAGACTCTGG ATGAGAAATT GAGGAATTTA
    TTGTACCAAG AGTACGCTCC GATGTACCCA TCGGGAAGTG CAGCTGAAAC TCCAGGATCT
    GGTACAGAGT ATATCCAGTC TCCACCAGGA CCTGAGAGTG CCATGGGAGG GTCAGGAACC
    AGCACACCTA GTCTTATAGG AGAGGGACGA TTCAGGGCAG GTGAACAGCT GCCACAGATC
    CCAGAGCGGG TAGACAGTTT AAGCGCTCTC AGCGACTCTG CAGTTGGAGT TACTGTGTCA
    AGGAGACATG TGATGCCACA CTCTACATCT TGTTCTGGAT CTAGAAATCG ATATAAGATG
    GTGCCAAGCT CCACTGACAT CTTGACAGGT CAAGGTCGAA AGCAGCGCAG CCTGAGCAGC
    ACGGCATCTC CAGCACACCC TGGTGGATTT TTGGGAGAAT GTACCATGTA CGAAGAGCCG
    ACTATGTCAG CTACTACAGT TGGCAGGTTC TCAGTGGTCA GCACAGAAGA CGAGGTCACG
    CGTAGAAAGT ACAGTAGCAG ATATTCAGCT CCACCTGACT TTTATCTGGA CGCTCCACCT
    CCACTGACCA AACGGGGGTC TTTGCCAAGG GCGCAGACCT CGACTTCAGC AGATGCCACA
    GTTTGCACAC GATTTATGTC ATCTGACTCT GGGGCAGAAA GCAGCCCTGC AAAAATGGCA
    CCTACAACCC CATCCCGGCA TGGCAGGTCT GAAAGGAGAG GAAGCGACCT CATGAAAAGG
    GCAGTGGCTT TTCTAAAGCG CTCTGGCCGC AGCAGTAGTG TGCAAAGCTC TGATTCACCA
    AGCCGGAAAG GAGGGGTGTA TGGATCCTAT GTCAGCAGTG ACAATGACTC AGAGATGGAG
    GACTCAGATA TTAAAAAGGA GCTTCAAAAT CTAAGAGAAA AGCATATGAA GGAGATAAGT
    GAATTGGAGG CCCACCACAG AGAAGAGATT GAGCTTCTCT ATGTCAGATT AGGAAAACCA
    CCACCTCCAG GTCTATACAT CCCACCCACA GTGCCCCCTG CTGGACGCAA GCGCAGGACC
    AGCAGGCACA AACTAAAAGC TGGCAAACTG CTCAGCCCTC TAGTACAACA ACTGAGAAAT
    GTTGCTTCTA AAACTGGTGA CTCCAGCAAA CCTAATGATT TAACCAAATC TGCTGAGGCG
    GTCCCGAGCT TGAATGGATC TCCTGTCAGA GCATCCAACT TGTCTGATGG CAGGCCTTAT
    TCTGGGACCG GGACTCTGCC TTGTTCTGTT TCTGAGCCAG TTCAGACCCA ACAGCCCTGC
    TCTCTTAAAG GATCTCTGTC TTCCGACAAT ATCTACTATG GTGCACAGGG AGAAGGACCC
    GTGCCACGCA GCCAGCCTGC CCAAGGCTGG CCTCCTTCTC CCCAGGCGTC TGCGCAGGTC
    ACCTATAAAT CCAGTAGTAA ACCACGCGCT AGATTCCTCA GTGGGCCAGT TTCTCTGTCC
    ATCTGGTCAA CACTTAAGCG CCTGTGTCTG GGTAAAGAAC GTGGCAGCAG ATCTGGAGCA
    TCATCAGGTG CCTCAAATCA ATCCCCTATG CCTCCTGGAT CCACTCCTCC TCCTCACCAG
    CCTGTTGGCC TTGCCCAAGC ACAGACAAAC AACAGCAACA ACAAAACAGA TGCTTTCACT
    CAGCAGCTTC ACCGACTGGT GGATAACTGG GCAGAAGGTG TGAGGGCTCC TTCACGCTCA
    CGCTCCCTCA GTCTAAGACC ACAGCAACTG ACCAGAACCA GGATCTGGAG TGCAACAGAG
    GTTCCAGGGT CAATGGAAAG ATTGAATGCT TCCCGGCTGC CTCTTTCATG GCCACAGATT
    GATTTCCATG CCTCTGTGAT GGTGACTGAC ACTTCACAAG TGCTCCAGCA CAGTTTTATG
    GTGTCCAGTA ACCCTTATGA AAAGATGCTT AACTCCACGC ACTTTGACAA GGACCTTTGG
    CCAGCAATGA CTGCCAATCT TAACCAAGAA GTCTTTGCTT TCTCTGCAGC GGACTCTCCG
    TCCTGGACAG CACCTTCTTC CCCCAGCGAG GGTCCTAGTT CTAGAAATAG GACCATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304505.1
    CDS712..8511
    Translation

    Target ORF information:

    RefSeq Version XM_009306230.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306230.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GTTTCCTTCG ACATATCCTC TTTTACCAGA AGGGGGCACA
    TCTACAGGGA CAGAACCTAT ATCTTTCTCC TCAGTCTCTT ACTCTTCTCC TTATCCCACA
    AATGCTCCTC CTGCATCATC CCCCTACTAC TCACCAAGCC CCATCAGCGC TCCCCTTCCC
    ATAATACCCA TGCAGAATGT ACCTTGCATA CTAGGATCAG GCACACCTGT ATCCACCCCT
    CTCAATGTTC CCACCCCTAT ACCTCTCCTG GCTATGGCCC TCTCCCCACC CATGCCTCCA
    CCAGAGCAAC ATCTGCAACA GATGTTCTCC CCAATTCCAA CACCAGAAGG TGCCCTTTAC
    CAACCTCAAC CCCAACCAAC ACAGCCCTCC CTTCCCCTTC CAAATCCAGC ATCTCTCCCT
    CAGCAAGACC CGTCTATTCC TGAACCTTTT AAAGAGGAAT ACTCTCACCA TGAGGAATTG
    CAGATCCTGG CTCCATCTCA CACTGTTATG TCACAGATTC AGTCTCCTCC ACAGGACAGT
    AAGTCAGAAA CAAAGTTTAC CAATGTCCAA GCCTTTTCTG AGACAGTGGA GCATCCTCTG
    CAGTCTACTG GCATTCAGAG AGCTTATGCT GTTCCTGAGA GTACTGGCAC AGCTAGTGCA
    ACTCAGCAAA GGGCAGAAAC TTCTGCAATG CCCAAACTCC CAGCACCAGA GCCCTGCCCA
    TTTCAACACA GCACTGAGGC ACCTGCATCT ATTCCTCTCC ATGCTTTTGT GTGCGACAGC
    CTAAATCAAG ATGCAGGATC TAGTAAAGAA ATGAGTGACA GCTATGAAGG ATCCACCGGT
    GGAGGAAAAG GTGATGGCAA ACCCAGAAAG CATCACCGCA AATCTGCTCG CACACGTTCA
    CGCCAAGAAA AAATTAACAA GCCAAAACTA AGCATGTTAA ATGTTAGTAA TACGGGTGAC
    AAGATGGTGG AATGCCAGTT GGAGACACAC AATCATAAAA TGGTGACTTT CAAGTTCGAC
    CTAGATGGAG ATGCGCCTGA AGAAATAGCC ACTTATATGG TGGAAAATGG TTTCATTCTA
    CCAATAGAAA AGGAGATTTT CATAGATCAA CTAAAAGACA TTGTTGACAA GGCAGAGGAT
    ATTTTAAGTG AGGACATGGA TGGAGAGAAG ACATCAGAAC TTGGAAAAGG ACAGACACCT
    GGAGATAAAG GAAGAGAGGC AATAAATGGA CAGCAACCTG GGATTCCCCA GGCAGTTTAT
    CAGCAAAATG TTCTGCACAC AGGCAAGAGG TGGTTCATCA TCTGCCCTGT AGAAGAAGCT
    CCTGCAGCAA GTCAGGATGG ATGCTCAGAT GGAGGTCCAT CTACACAATC TCCATCTACA
    ACAACAATAA CTGAAGGAAC AGCCACTGCT CAGCCTGTAG AGAGTGCATC AGTACAACCA
    CCAGAACCCA GCACAGGATC TGCTTCCGCT TCTATGGATT CAGAAACCTG TGCCACAGCG
    CCTCCATCTG GAGGAAGTGA TCCCTATGGG GTCTGGAGCC CTCTCTCTTT GACAAATACT
    GATCCTCTTT CTCTGGCTGC TCTTTCCCAA GCTCCTCCTG CCCCGCAAGC AGCTATGATG
    CCAGCACAGC CTGCTTCACA TTCAGAGGAG GGCCAAAATT TGGGCTCTAC CCAGGTCAGT
    GTACAGCAGT CCCAGCCCTG CATAGATCCT CAAAGCTCTC TTTTTGTGGA AGAGACTCAA
    AGTGGCAGAT TAGACTCGGT CTCCGCAATG CACACCGCTC AGCAAATGGC TGAAATTGCA
    TGTGCTGCCT CATTGGTGGA GGACGTGCCC TGCTGTCCCT TGGTCATGCC GTTGTCCCTT
    GAAGTAAGCA GCGGGGCTCA GAAGCCATCA ACCGTAATTC CGCCACCATC ACAAGACAGT
    ACATCTGCTC GCGAACAACT CCAATCCATT ACATCTAATC GAGCGGAGCG TGGCCTGCAA
    CCTGTGGTGC TGCAGCAGCC TGTTTCCACT GTAAGTGGAT CTAAGGCTCC TTCCCTGCCT
    CAGAGCCCTG CTCCTTCACA GCACCACTTT GTCCCTGGTG AGTCAGATGG AGAGGGGCGT
    AGCAGAGGAG GGTTTGTAGA CAGCACTATC AAGACTCTGG ATGAGAAATT GAGGAATTTA
    TTGTACCAAG AGTACGCTCC GATGTACCCA TCGGGAAGTG CAGCTGAAAC TCCAGGATCT
    GGTACAGAGT ATATCCAGTC TCCACCAGGA CCTGAGAGTG CCATGGGAGG GTCAGGAACC
    AGCACACCTA GTCTTATAGG AGAGGGACGA TTCAGGGCAG GTGAACAGCT GCCACAGATC
    CCAGAGCGGG TAGACAGTTT AAGCGCTCTC AGCGACTCTG CAGTTGGAGT TACTGTGTCA
    AGGAGACATG TGATGCCACA CTCTACATCT TGTTCTGGAT CTAGAAATCG ATATAAGATG
    GTGCCAAGCT CCACTGACAT CTTGACAGGT CAAGGTCGAA AGCAGCGCAG CCTGAGCAGC
    ACGGCATCTC CAGCACACCC TGGTGGATTT TTGGGAGAAT GTACCATGTA CGAAGAGCCG
    ACTATGTCAG CTACTACAGT TGGCAGGTTC TCAGTGGTCA GCACAGAAGA CGAGGTCACG
    CGTAGAAAGT ACAGTAGCAG ATATTCAGCT CCACCTGACT TTTATCTGGA CGCTCCACCT
    CCACTGACCA AACGGGGGTC TTTGCCAAGG GCGCAGACCT CGACTTCAGC AGATGCCACA
    GTTTGCACAC GATTTATGTC ATCTGACTCT GGGGCAGAAA GCAGCCCTGC AAAAATGGCA
    CCTACAACCC CATCCCGGCA TGGCAGGTCT GAAAGGAGAG GAAGCGACCT CATGAAAAGG
    GCAGTGGCTT TTCTAAAGCG CTCTGGCCGC AGCAGTAGTG TGCAAAGCTC TGATTCACCA
    AGCCGGAAAG GAGGGGTGTA TGGATCCTAT GTCAGCAGTG ACAATGACTC AGAGATGGAG
    GACTCAGATA TTAAAAAGGA GCTTCAAAAT CTAAGAGAAA AGCATATGAA GGAGATAAGT
    GAATTGGAGG CCCACCACAG AGAAGAGATT GAGCTTCTCT ATGTCAGATT AGGAAAACCA
    CCACCTCCAG GTCTATACAT CCCACCCACA GTGCCCCCTG CTGGACGCAA GCGCAGGACC
    AGCAGGCACA AACTAAAAGC TGGCAAACTG CTCAGCCCTC TAGTACAACA ACTGAGAAAT
    GTTGCTTCTA AAACTGGTGA CTCCAGCAAA CCTAATGATT TAACCAAATC TGCTGAGGCG
    GTCCCGAGCT TGAATGGATC TCCTGTCAGA GCATCCAACT TGTCTGATGG CAGGCCTTAT
    TCTGGGACCG GGACTCTGCC TTGTTCTGTT TCTGAGCCAG TTCAGACCCA ACAGCCCTGC
    TCTCTTAAAG GATCTCTGTC TTCCGACAAT ATCTACTATG GTGCACAGGG AGAAGGACCC
    GTGCCACGCA GCCAGCCTGC CCAAGGCTGG CCTCCTTCTC CCCAGGCGTC TGCGCAGGTC
    ACCTATAAAT CCAGTAGTAA ACCACGCGCT AGATTCCTCA GTGGGCCAGT TTCTCTGTCC
    ATCTGGTCAA CACTTAAGCG CCTGTGTCTG GGTAAAGAAC GTGGCAGCAG ATCTGGAGCA
    TCATCAGGTG CCTCAAATCA ATCCCCTATG CCTCCTGGAT CCACTCCTCC TCCTCACCAG
    CCTGTTGGCC TTGCCCAAGC ACAGACAAAC AACAGCAACA ACAAAACAGA TGCTTTCACT
    CAGCAGCTTC ACCGACTGGT GGATAACTGG GCAGAAGGTG TGAGGGCTCC TTCACGCTCA
    CGCTCCCTCA GTCTAAGACC ACAGCAACTG ACCAGAACCA GGATCTGGAG TGCAACAGAG
    GTTCCAGGGT CAATGGAAAG ATTGAATGCT TCCCGGCTGC CTCTTTCATG GCCACAGATT
    GATTTCCATG CCTCTGTGAT GGTGACTGAC ACTTCACAAG TGCTCCAGCA CAGTTTTATG
    GTGTCCAGTA ACCCTTATGA AAAGATGCTT AACTCCACGC ACTTTGACAA GGACCTTTGG
    CCAGCAATGA CTGCCAATCT TAACCAAGAA GTCTTTGCTT TCTCTGCAGC GGACTCTCCG
    TCCTGGACAG CACCTTCTTC CCCCAGCGAG GGTCCTAGTT CTAGAAATAG GACCATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53084
    Clone ID Related Accession (Same CDS sequence) XM_009306230.2 , XM_009306231.2
    Accession Version XM_009306231.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306231.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GTTTCCTTCG ACATATCCTC TTTTACCAGA AGGGGGCACA
    TCTACAGGGA CAGAACCTAT ATCTTTCTCC TCAGTCTCTT ACTCTTCTCC TTATCCCACA
    AATGCTCCTC CTGCATCATC CCCCTACTAC TCACCAAGCC CCATCAGCGC TCCCCTTCCC
    ATAATACCCA TGCAGAATGT ACCTTGCATA CTAGGATCAG GCACACCTGT ATCCACCCCT
    CTCAATGTTC CCACCCCTAT ACCTCTCCTG GCTATGGCCC TCTCCCCACC CATGCCTCCA
    CCAGAGCAAC ATCTGCAACA GATGTTCTCC CCAATTCCAA CACCAGAAGG TGCCCTTTAC
    CAACCTCAAC CCCAACCAAC ACAGCCCTCC CTTCCCCTTC CAAATCCAGC ATCTCTCCCT
    CAGCAAGACC CGTCTATTCC TGAACCTTTT AAAGAGGAAT ACTCTCACCA TGAGGAATTG
    CAGATCCTGG CTCCATCTCA CACTGTTATG TCACAGATTC AGTCTCCTCC ACAGGACAGT
    AAGTCAGAAA CAAAGTTTAC CAATGTCCAA GCCTTTTCTG AGACAGTGGA GCATCCTCTG
    CAGTCTACTG GCATTCAGAG AGCTTATGCT GTTCCTGAGA GTACTGGCAC AGCTAGTGCA
    ACTCAGCAAA GGGCAGAAAC TTCTGCAATG CCCAAACTCC CAGCACCAGA GCCCTGCCCA
    TTTCAACACA GCACTGAGGC ACCTGCATCT ATTCCTCTCC ATGCTTTTGT GTGCGACAGC
    CTAAATCAAG ATGCAGGATC TAGTAAAGAA ATGAGTGACA GCTATGAAGG ATCCACCGGT
    GGAGGAAAAG GTGATGGCAA ACCCAGAAAG CATCACCGCA AATCTGCTCG CACACGTTCA
    CGCCAAGAAA AAATTAACAA GCCAAAACTA AGCATGTTAA ATGTTAGTAA TACGGGTGAC
    AAGATGGTGG AATGCCAGTT GGAGACACAC AATCATAAAA TGGTGACTTT CAAGTTCGAC
    CTAGATGGAG ATGCGCCTGA AGAAATAGCC ACTTATATGG TGGAAAATGG TTTCATTCTA
    CCAATAGAAA AGGAGATTTT CATAGATCAA CTAAAAGACA TTGTTGACAA GGCAGAGGAT
    ATTTTAAGTG AGGACATGGA TGGAGAGAAG ACATCAGAAC TTGGAAAAGG ACAGACACCT
    GGAGATAAAG GAAGAGAGGC AATAAATGGA CAGCAACCTG GGATTCCCCA GGCAGTTTAT
    CAGCAAAATG TTCTGCACAC AGGCAAGAGG TGGTTCATCA TCTGCCCTGT AGAAGAAGCT
    CCTGCAGCAA GTCAGGATGG ATGCTCAGAT GGAGGTCCAT CTACACAATC TCCATCTACA
    ACAACAATAA CTGAAGGAAC AGCCACTGCT CAGCCTGTAG AGAGTGCATC AGTACAACCA
    CCAGAACCCA GCACAGGATC TGCTTCCGCT TCTATGGATT CAGAAACCTG TGCCACAGCG
    CCTCCATCTG GAGGAAGTGA TCCCTATGGG GTCTGGAGCC CTCTCTCTTT GACAAATACT
    GATCCTCTTT CTCTGGCTGC TCTTTCCCAA GCTCCTCCTG CCCCGCAAGC AGCTATGATG
    CCAGCACAGC CTGCTTCACA TTCAGAGGAG GGCCAAAATT TGGGCTCTAC CCAGGTCAGT
    GTACAGCAGT CCCAGCCCTG CATAGATCCT CAAAGCTCTC TTTTTGTGGA AGAGACTCAA
    AGTGGCAGAT TAGACTCGGT CTCCGCAATG CACACCGCTC AGCAAATGGC TGAAATTGCA
    TGTGCTGCCT CATTGGTGGA GGACGTGCCC TGCTGTCCCT TGGTCATGCC GTTGTCCCTT
    GAAGTAAGCA GCGGGGCTCA GAAGCCATCA ACCGTAATTC CGCCACCATC ACAAGACAGT
    ACATCTGCTC GCGAACAACT CCAATCCATT ACATCTAATC GAGCGGAGCG TGGCCTGCAA
    CCTGTGGTGC TGCAGCAGCC TGTTTCCACT GTAAGTGGAT CTAAGGCTCC TTCCCTGCCT
    CAGAGCCCTG CTCCTTCACA GCACCACTTT GTCCCTGGTG AGTCAGATGG AGAGGGGCGT
    AGCAGAGGAG GGTTTGTAGA CAGCACTATC AAGACTCTGG ATGAGAAATT GAGGAATTTA
    TTGTACCAAG AGTACGCTCC GATGTACCCA TCGGGAAGTG CAGCTGAAAC TCCAGGATCT
    GGTACAGAGT ATATCCAGTC TCCACCAGGA CCTGAGAGTG CCATGGGAGG GTCAGGAACC
    AGCACACCTA GTCTTATAGG AGAGGGACGA TTCAGGGCAG GTGAACAGCT GCCACAGATC
    CCAGAGCGGG TAGACAGTTT AAGCGCTCTC AGCGACTCTG CAGTTGGAGT TACTGTGTCA
    AGGAGACATG TGATGCCACA CTCTACATCT TGTTCTGGAT CTAGAAATCG ATATAAGATG
    GTGCCAAGCT CCACTGACAT CTTGACAGGT CAAGGTCGAA AGCAGCGCAG CCTGAGCAGC
    ACGGCATCTC CAGCACACCC TGGTGGATTT TTGGGAGAAT GTACCATGTA CGAAGAGCCG
    ACTATGTCAG CTACTACAGT TGGCAGGTTC TCAGTGGTCA GCACAGAAGA CGAGGTCACG
    CGTAGAAAGT ACAGTAGCAG ATATTCAGCT CCACCTGACT TTTATCTGGA CGCTCCACCT
    CCACTGACCA AACGGGGGTC TTTGCCAAGG GCGCAGACCT CGACTTCAGC AGATGCCACA
    GTTTGCACAC GATTTATGTC ATCTGACTCT GGGGCAGAAA GCAGCCCTGC AAAAATGGCA
    CCTACAACCC CATCCCGGCA TGGCAGGTCT GAAAGGAGAG GAAGCGACCT CATGAAAAGG
    GCAGTGGCTT TTCTAAAGCG CTCTGGCCGC AGCAGTAGTG TGCAAAGCTC TGATTCACCA
    AGCCGGAAAG GAGGGGTGTA TGGATCCTAT GTCAGCAGTG ACAATGACTC AGAGATGGAG
    GACTCAGATA TTAAAAAGGA GCTTCAAAAT CTAAGAGAAA AGCATATGAA GGAGATAAGT
    GAATTGGAGG CCCACCACAG AGAAGAGATT GAGCTTCTCT ATGTCAGATT AGGAAAACCA
    CCACCTCCAG GTCTATACAT CCCACCCACA GTGCCCCCTG CTGGACGCAA GCGCAGGACC
    AGCAGGCACA AACTAAAAGC TGGCAAACTG CTCAGCCCTC TAGTACAACA ACTGAGAAAT
    GTTGCTTCTA AAACTGGTGA CTCCAGCAAA CCTAATGATT TAACCAAATC TGCTGAGGCG
    GTCCCGAGCT TGAATGGATC TCCTGTCAGA GCATCCAACT TGTCTGATGG CAGGCCTTAT
    TCTGGGACCG GGACTCTGCC TTGTTCTGTT TCTGAGCCAG TTCAGACCCA ACAGCCCTGC
    TCTCTTAAAG GATCTCTGTC TTCCGACAAT ATCTACTATG GTGCACAGGG AGAAGGACCC
    GTGCCACGCA GCCAGCCTGC CCAAGGCTGG CCTCCTTCTC CCCAGGCGTC TGCGCAGGTC
    ACCTATAAAT CCAGTAGTAA ACCACGCGCT AGATTCCTCA GTGGGCCAGT TTCTCTGTCC
    ATCTGGTCAA CACTTAAGCG CCTGTGTCTG GGTAAAGAAC GTGGCAGCAG ATCTGGAGCA
    TCATCAGGTG CCTCAAATCA ATCCCCTATG CCTCCTGGAT CCACTCCTCC TCCTCACCAG
    CCTGTTGGCC TTGCCCAAGC ACAGACAAAC AACAGCAACA ACAAAACAGA TGCTTTCACT
    CAGCAGCTTC ACCGACTGGT GGATAACTGG GCAGAAGGTG TGAGGGCTCC TTCACGCTCA
    CGCTCCCTCA GTCTAAGACC ACAGCAACTG ACCAGAACCA GGATCTGGAG TGCAACAGAG
    GTTCCAGGGT CAATGGAAAG ATTGAATGCT TCCCGGCTGC CTCTTTCATG GCCACAGATT
    GATTTCCATG CCTCTGTGAT GGTGACTGAC ACTTCACAAG TGCTCCAGCA CAGTTTTATG
    GTGTCCAGTA ACCCTTATGA AAAGATGCTT AACTCCACGC ACTTTGACAA GGACCTTTGG
    CCAGCAATGA CTGCCAATCT TAACCAAGAA GTCTTTGCTT TCTCTGCAGC GGACTCTCCG
    TCCTGGACAG CACCTTCTTC CCCCAGCGAG GGTCCTAGTT CTAGAAATAG GACCATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304506.1
    CDS847..8646
    Translation

    Target ORF information:

    RefSeq Version XM_009306231.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306231.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GTTTCCTTCG ACATATCCTC TTTTACCAGA AGGGGGCACA
    TCTACAGGGA CAGAACCTAT ATCTTTCTCC TCAGTCTCTT ACTCTTCTCC TTATCCCACA
    AATGCTCCTC CTGCATCATC CCCCTACTAC TCACCAAGCC CCATCAGCGC TCCCCTTCCC
    ATAATACCCA TGCAGAATGT ACCTTGCATA CTAGGATCAG GCACACCTGT ATCCACCCCT
    CTCAATGTTC CCACCCCTAT ACCTCTCCTG GCTATGGCCC TCTCCCCACC CATGCCTCCA
    CCAGAGCAAC ATCTGCAACA GATGTTCTCC CCAATTCCAA CACCAGAAGG TGCCCTTTAC
    CAACCTCAAC CCCAACCAAC ACAGCCCTCC CTTCCCCTTC CAAATCCAGC ATCTCTCCCT
    CAGCAAGACC CGTCTATTCC TGAACCTTTT AAAGAGGAAT ACTCTCACCA TGAGGAATTG
    CAGATCCTGG CTCCATCTCA CACTGTTATG TCACAGATTC AGTCTCCTCC ACAGGACAGT
    AAGTCAGAAA CAAAGTTTAC CAATGTCCAA GCCTTTTCTG AGACAGTGGA GCATCCTCTG
    CAGTCTACTG GCATTCAGAG AGCTTATGCT GTTCCTGAGA GTACTGGCAC AGCTAGTGCA
    ACTCAGCAAA GGGCAGAAAC TTCTGCAATG CCCAAACTCC CAGCACCAGA GCCCTGCCCA
    TTTCAACACA GCACTGAGGC ACCTGCATCT ATTCCTCTCC ATGCTTTTGT GTGCGACAGC
    CTAAATCAAG ATGCAGGATC TAGTAAAGAA ATGAGTGACA GCTATGAAGG ATCCACCGGT
    GGAGGAAAAG GTGATGGCAA ACCCAGAAAG CATCACCGCA AATCTGCTCG CACACGTTCA
    CGCCAAGAAA AAATTAACAA GCCAAAACTA AGCATGTTAA ATGTTAGTAA TACGGGTGAC
    AAGATGGTGG AATGCCAGTT GGAGACACAC AATCATAAAA TGGTGACTTT CAAGTTCGAC
    CTAGATGGAG ATGCGCCTGA AGAAATAGCC ACTTATATGG TGGAAAATGG TTTCATTCTA
    CCAATAGAAA AGGAGATTTT CATAGATCAA CTAAAAGACA TTGTTGACAA GGCAGAGGAT
    ATTTTAAGTG AGGACATGGA TGGAGAGAAG ACATCAGAAC TTGGAAAAGG ACAGACACCT
    GGAGATAAAG GAAGAGAGGC AATAAATGGA CAGCAACCTG GGATTCCCCA GGCAGTTTAT
    CAGCAAAATG TTCTGCACAC AGGCAAGAGG TGGTTCATCA TCTGCCCTGT AGAAGAAGCT
    CCTGCAGCAA GTCAGGATGG ATGCTCAGAT GGAGGTCCAT CTACACAATC TCCATCTACA
    ACAACAATAA CTGAAGGAAC AGCCACTGCT CAGCCTGTAG AGAGTGCATC AGTACAACCA
    CCAGAACCCA GCACAGGATC TGCTTCCGCT TCTATGGATT CAGAAACCTG TGCCACAGCG
    CCTCCATCTG GAGGAAGTGA TCCCTATGGG GTCTGGAGCC CTCTCTCTTT GACAAATACT
    GATCCTCTTT CTCTGGCTGC TCTTTCCCAA GCTCCTCCTG CCCCGCAAGC AGCTATGATG
    CCAGCACAGC CTGCTTCACA TTCAGAGGAG GGCCAAAATT TGGGCTCTAC CCAGGTCAGT
    GTACAGCAGT CCCAGCCCTG CATAGATCCT CAAAGCTCTC TTTTTGTGGA AGAGACTCAA
    AGTGGCAGAT TAGACTCGGT CTCCGCAATG CACACCGCTC AGCAAATGGC TGAAATTGCA
    TGTGCTGCCT CATTGGTGGA GGACGTGCCC TGCTGTCCCT TGGTCATGCC GTTGTCCCTT
    GAAGTAAGCA GCGGGGCTCA GAAGCCATCA ACCGTAATTC CGCCACCATC ACAAGACAGT
    ACATCTGCTC GCGAACAACT CCAATCCATT ACATCTAATC GAGCGGAGCG TGGCCTGCAA
    CCTGTGGTGC TGCAGCAGCC TGTTTCCACT GTAAGTGGAT CTAAGGCTCC TTCCCTGCCT
    CAGAGCCCTG CTCCTTCACA GCACCACTTT GTCCCTGGTG AGTCAGATGG AGAGGGGCGT
    AGCAGAGGAG GGTTTGTAGA CAGCACTATC AAGACTCTGG ATGAGAAATT GAGGAATTTA
    TTGTACCAAG AGTACGCTCC GATGTACCCA TCGGGAAGTG CAGCTGAAAC TCCAGGATCT
    GGTACAGAGT ATATCCAGTC TCCACCAGGA CCTGAGAGTG CCATGGGAGG GTCAGGAACC
    AGCACACCTA GTCTTATAGG AGAGGGACGA TTCAGGGCAG GTGAACAGCT GCCACAGATC
    CCAGAGCGGG TAGACAGTTT AAGCGCTCTC AGCGACTCTG CAGTTGGAGT TACTGTGTCA
    AGGAGACATG TGATGCCACA CTCTACATCT TGTTCTGGAT CTAGAAATCG ATATAAGATG
    GTGCCAAGCT CCACTGACAT CTTGACAGGT CAAGGTCGAA AGCAGCGCAG CCTGAGCAGC
    ACGGCATCTC CAGCACACCC TGGTGGATTT TTGGGAGAAT GTACCATGTA CGAAGAGCCG
    ACTATGTCAG CTACTACAGT TGGCAGGTTC TCAGTGGTCA GCACAGAAGA CGAGGTCACG
    CGTAGAAAGT ACAGTAGCAG ATATTCAGCT CCACCTGACT TTTATCTGGA CGCTCCACCT
    CCACTGACCA AACGGGGGTC TTTGCCAAGG GCGCAGACCT CGACTTCAGC AGATGCCACA
    GTTTGCACAC GATTTATGTC ATCTGACTCT GGGGCAGAAA GCAGCCCTGC AAAAATGGCA
    CCTACAACCC CATCCCGGCA TGGCAGGTCT GAAAGGAGAG GAAGCGACCT CATGAAAAGG
    GCAGTGGCTT TTCTAAAGCG CTCTGGCCGC AGCAGTAGTG TGCAAAGCTC TGATTCACCA
    AGCCGGAAAG GAGGGGTGTA TGGATCCTAT GTCAGCAGTG ACAATGACTC AGAGATGGAG
    GACTCAGATA TTAAAAAGGA GCTTCAAAAT CTAAGAGAAA AGCATATGAA GGAGATAAGT
    GAATTGGAGG CCCACCACAG AGAAGAGATT GAGCTTCTCT ATGTCAGATT AGGAAAACCA
    CCACCTCCAG GTCTATACAT CCCACCCACA GTGCCCCCTG CTGGACGCAA GCGCAGGACC
    AGCAGGCACA AACTAAAAGC TGGCAAACTG CTCAGCCCTC TAGTACAACA ACTGAGAAAT
    GTTGCTTCTA AAACTGGTGA CTCCAGCAAA CCTAATGATT TAACCAAATC TGCTGAGGCG
    GTCCCGAGCT TGAATGGATC TCCTGTCAGA GCATCCAACT TGTCTGATGG CAGGCCTTAT
    TCTGGGACCG GGACTCTGCC TTGTTCTGTT TCTGAGCCAG TTCAGACCCA ACAGCCCTGC
    TCTCTTAAAG GATCTCTGTC TTCCGACAAT ATCTACTATG GTGCACAGGG AGAAGGACCC
    GTGCCACGCA GCCAGCCTGC CCAAGGCTGG CCTCCTTCTC CCCAGGCGTC TGCGCAGGTC
    ACCTATAAAT CCAGTAGTAA ACCACGCGCT AGATTCCTCA GTGGGCCAGT TTCTCTGTCC
    ATCTGGTCAA CACTTAAGCG CCTGTGTCTG GGTAAAGAAC GTGGCAGCAG ATCTGGAGCA
    TCATCAGGTG CCTCAAATCA ATCCCCTATG CCTCCTGGAT CCACTCCTCC TCCTCACCAG
    CCTGTTGGCC TTGCCCAAGC ACAGACAAAC AACAGCAACA ACAAAACAGA TGCTTTCACT
    CAGCAGCTTC ACCGACTGGT GGATAACTGG GCAGAAGGTG TGAGGGCTCC TTCACGCTCA
    CGCTCCCTCA GTCTAAGACC ACAGCAACTG ACCAGAACCA GGATCTGGAG TGCAACAGAG
    GTTCCAGGGT CAATGGAAAG ATTGAATGCT TCCCGGCTGC CTCTTTCATG GCCACAGATT
    GATTTCCATG CCTCTGTGAT GGTGACTGAC ACTTCACAAG TGCTCCAGCA CAGTTTTATG
    GTGTCCAGTA ACCCTTATGA AAAGATGCTT AACTCCACGC ACTTTGACAA GGACCTTTGG
    CCAGCAATGA CTGCCAATCT TAACCAAGAA GTCTTTGCTT TCTCTGCAGC GGACTCTCCG
    TCCTGGACAG CACCTTCTTC CCCCAGCGAG GGTCCTAGTT CTAGAAATAG GACCATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53085
    Clone ID Related Accession (Same CDS sequence) XM_009306232.2
    Accession Version XM_009306232.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306232.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GTTTCCTTCG ACATATCCTC TTTTACCAGA AGGGGGCACA
    TCTACAGGGA CAGAACCTAT ATCTTTCTCC TCAGTCTCTT ACTCTTCTCC TTATCCCACA
    AATGCTCCTC CTGCATCATC CCCCTACTAC TCACCAAGCC CCATCAGCGC TCCCCTTCCC
    ATAATACCCA TGCAGAATGT ACCTTGCATA CTAGGATCAG GCACACCTGT ATCCACCCCT
    CTCAATGTTC CCACCCCTAT ACCTCTCCTG GCTATGGCCC TCTCCCCACC CATGCCTCCA
    CCAGAGCAAC ATCTGCAACA GATGTTCTCC CCAATTCCAA CACCAGAAGG TGCCCTTTAC
    CAACCTCAAC CCCAACCAAC ACAGCCCTCC CTTCCCCTTC CAAATCCAGC ATCTCTCCCT
    CAGCAAGACC CGTCTATTCC TGAACCTTTT AAAGAGGAAT ACTCTCACCA TGAGGAATTG
    CAGATCCTGG CTCCATCTCA CACTGTTATG TCACAGATTC AGTCTCCTCC ACAGGACAGT
    AAGTCAGAAA CAAAGTTTAC CAATGTCCAA GCCTTTTCTG AGACAGTGGA GCATCCTCTG
    CAGTCTACTG GCATTCAGAG AGCTTATGCT GTTCCTGAGA GTACTGGCAC AGCTAGTGCA
    ACTCAGCAAA GGGCAGAAAC TTCTGCAATG CCCAAACTCC CAGCACCAGA GCCCTGCCCA
    TTTCAACACA GCACTGAGGC ACCTGCATCT ATTCCTCTCC ATGCTTTTGT GTGCGACAGC
    CTAAATCAAG ATGCAGGATC TAGTAAAGAA ATGAGTGACA GCTATGAAGG ATCCACCGGT
    GGAGGAAAAG GTGATGGCAA ACCCAGAAAG CATCACCGCA AATCTGCTCG CACACGTTCA
    CGCCAAGAAA AAATTAACAA GCCAAAACTA AGCATGTTAA ATGTTAGTAA TACGGGTGAC
    AAGATGGTGG AATGCCAGTT GGAGACACAC AATCATAAAA TGGTGACTTT CAAGTTCGAC
    CTAGATGGAG ATGCGCCTGA AGAAATAGCC ACTTATATGG TGGAAAATGG TTTCATTCTA
    CCAATAGAAA AGGAGATTTT CATAGATCAA CTAAAAGACA TTGTTGACAA GGCAGAGGAT
    ATTTTAAGTG AGGACATGGA TGGAGAGAAG ACATCAGAAC TTGGAAAAGG ACAGACACCT
    GGAGATAAAG GAAGAGAGGC AATAAATGGA CAGCAACCTG GGATTCCCCA GGCAGTTTAT
    CAGCAAAATG TTCTGCACAC AGGCAAGAGG TGGTTCATCA TCTGCCCTGT AGAAGAAGCT
    CCTGCAGCAA GTCAGGATGG ATGCTCAGAT GGAGGTCCAT CTACACAATC TCCATCTACA
    ACAACAATAA CTGAAGGAAC AGCCACTGCT CAGCCTGTAG AGAGTGCATC AGTACAACCA
    CCAGAACCCA GCACAGGATC TGCTTCCGCT TCTATGGATT CAGAAACCTG TGCCACAGCG
    CCTCCATCTG GAGGAAGTGA TCCCTATGGG GTCTGGAGCC CTCTCTCTTT GACAAATACT
    GATCCTCTTT CTCTGGCTGC TCTTTCCCAA GCTCCTCCTG CCCCGCAAGC AGCTATGATG
    CCAGCACAGC CTGCTTCACA TTCAGAGGAG GGCCAAAATT TGGGCTCTAC CCAGGTCAGT
    GTACAGCAGT CCCAGCCCTG CATAGATCCT CAAAGCTCTC TTTTTGTGGA AGAGACTCAA
    AGTGGCAGAT TAGACTCGGT CTCCGCAATG CACACCGCTC AGCAAATGGC TGAAATTGCA
    TGTGCTGCCT CATTGGTGGA GGACGTGCCC TGCTGTCCCT TGGTCATGCC GTTGTCCCTT
    GAAGTAAGCA GCGGGGCTCA GAAGCCATCA ACCGTAATTC CGCCACCATC ACAAGACAGT
    ACATCTGCTC GCGAACAACT CCAATCCATT ACATCTAATC GAGCGGAGCG TGGCCTGCAA
    CCTGTGGTGC TGCAGCAGCC TGTTTCCACT GTAAGTGGAT CTAAGGCTCC TTCCCTGCCT
    CAGAGCCCTG CTCCTTCACA GCACCACTTT GTCCCTGGTG AGTCAGATGG AGAGGGGCGT
    AGCAGAGGAG GGTTTGTAGA CAGCACTATC AAGACTCTGG ATGAGAAATT GAGGAATTTA
    TTGTACCAAG AGTACGCTCC GATGTACCCA TCGGGAAGTG CAGCTGAAAC TCCAGGATCT
    GGTACAGAGT ATATCCAGTC TCCACCAGGA CCTGAGAGTG CCATGGGAGG GTCAGGAACC
    AGCACACCTA GTCTTATAGG AGAGGGACGA TTCAGGGCAG GTGAACAGCT GCCACAGATC
    CCAGAGCGGG TAGACAGTTT AAGCGCTCTC AGCGACTCTG CAGTTGGAGT TACTGTGTCA
    AGGAGACATG TGATGCCACA CTCTACATCT TGTTCTGGAT CTAGAAATCG ATATAAGATG
    GTGCCAAGCT CCACTGACAT CTTGACAGGT CAAGGTCGAA AGCAGCGCAG CCTGAGCAGC
    ACGGCATCTC CAGCACACCC TGGTGGATTT TTGGGAGAAT GTACCATGTA CGAAGAGCCG
    ACTATGTCAG CTACTACAGT TGGCAGGTTC TCAGTGGTCA GCACAGAAGA CGAGGTCACG
    CGTAGAAAGT ACAGTAGCAG ATATTCAGCT CCACCTGACT TTTATCTGGA CGCTCCACCT
    CCACTGACCA AACGGGGGTC TTTGCCAAGG GCGCAGACCT CGACTTCAGC AGATGCCACA
    GTTTGCACAC GATTTATGTC ATCTGACTCT GGGGCAGAAA GCAGCCCTGC AAAAATGGCA
    CCTACAACCC CATCCCGGCA TGGCAGGTCT GAAAGGAGAG GAAGCGACCT CATGAAAAGG
    GCAGTGGCTT TTCTAAAGCG CTCTGGCCGC AGCAGTAGTG TGCAAAGCTC TGATTCACCA
    AGCCGGAAAG GAGGGGTGTA TGGATCCTAT GTCAGCAGTG ACAATGACTC AGAGATGGAG
    GACTCAGATA TTAAAAAGGA GCTTCAAAAT CTAAGAGAAA AGCATATGAA GGAGATAAGT
    GAATTGGAGG CCCACCACAG AGAAGAGATT GAGCTTCTCT ATGTCAGATT AGGAAAACCA
    CCACCTCCAG GTCTATACAT CCCACCCACA GTGCCCCCTG CTGGACGCAA GCGCAGGACC
    AGCAGGCACA AACTAAAAGC TGGCAAACTG CTCAGCCCTC TAGTACAACA ACTGAGAAAT
    GTTGCTTCTA AAACTGGTGA CTCCAGCAAA CCTAATGATT TAACCAAATC TGCTGAGGCG
    GTCCCGAGCT TGAATGGATC TCCTGTCAGA GCATCCAACT TGTCTGATGG CAGGCCTTAT
    TCTGGGACCG GGACTCTGCC TTGTTCTGTT TCTGAGCCAG TTCAGACCCA ACAGCCCTGC
    TCTCTTAAAG GATCTCTGTC TTCCGACAAT ATCTACTATG GTGCACAGGG AGAAGGACCC
    GTGCCACGCA GCCAGCCTGC CCAAGGCTGG CCTCCTTCTC CCCAGGCGTC TGCGCAGGTC
    ACCTATAAAT CCAGTAGTAA ACCACGCGCT AGATTCCTCA GGTCAACACT TAAGCGCCTG
    TGTCTGGGTA AAGAACGTGG CAGCAGATCT GGAGCATCAT CAGGTGCCTC AAATCAATCC
    CCTATGCCTC CTGGATCCAC TCCTCCTCCT CACCAGCCTG TTGGCCTTGC CCAAGCACAG
    ACAAACAACA GCAACAACAA AACAGATGCT TTCACTCAGC AGCTTCACCG ACTGGTGGAT
    AACTGGGCAG AAGGTGTGAG GGCTCCTTCA CGCTCACGCT CCCTCAGTCT AAGACCACAG
    CAACTGACCA GAACCAGGAT CTGGAGTGCA ACAGAGGTTC CAGGGTCAAT GGAAAGATTG
    AATGCTTCCC GGCTGCCTCT TTCATGGCCA CAGATTGATT TCCATGCCTC TGTGATGGTG
    ACTGACACTT CACAAGTGCT CCAGCACAGT TTTATGGTGT CCAGTAACCC TTATGAAAAG
    ATGCTTAACT CCACGCACTT TGACAAGGAC CTTTGGCCAG CAATGACTGC CAATCTTAAC
    CAAGAAGTCT TTGCTTTCTC TGCAGCGGAC TCTCCGTCCT GGACAGCACC TTCTTCCCCC
    AGCGAGGGTC CTAGTTCTAG AAATAGGACC ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304507.1
    CDS712..8487
    Translation

    Target ORF information:

    RefSeq Version XM_009306232.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306232.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GTTTCCTTCG ACATATCCTC TTTTACCAGA AGGGGGCACA
    TCTACAGGGA CAGAACCTAT ATCTTTCTCC TCAGTCTCTT ACTCTTCTCC TTATCCCACA
    AATGCTCCTC CTGCATCATC CCCCTACTAC TCACCAAGCC CCATCAGCGC TCCCCTTCCC
    ATAATACCCA TGCAGAATGT ACCTTGCATA CTAGGATCAG GCACACCTGT ATCCACCCCT
    CTCAATGTTC CCACCCCTAT ACCTCTCCTG GCTATGGCCC TCTCCCCACC CATGCCTCCA
    CCAGAGCAAC ATCTGCAACA GATGTTCTCC CCAATTCCAA CACCAGAAGG TGCCCTTTAC
    CAACCTCAAC CCCAACCAAC ACAGCCCTCC CTTCCCCTTC CAAATCCAGC ATCTCTCCCT
    CAGCAAGACC CGTCTATTCC TGAACCTTTT AAAGAGGAAT ACTCTCACCA TGAGGAATTG
    CAGATCCTGG CTCCATCTCA CACTGTTATG TCACAGATTC AGTCTCCTCC ACAGGACAGT
    AAGTCAGAAA CAAAGTTTAC CAATGTCCAA GCCTTTTCTG AGACAGTGGA GCATCCTCTG
    CAGTCTACTG GCATTCAGAG AGCTTATGCT GTTCCTGAGA GTACTGGCAC AGCTAGTGCA
    ACTCAGCAAA GGGCAGAAAC TTCTGCAATG CCCAAACTCC CAGCACCAGA GCCCTGCCCA
    TTTCAACACA GCACTGAGGC ACCTGCATCT ATTCCTCTCC ATGCTTTTGT GTGCGACAGC
    CTAAATCAAG ATGCAGGATC TAGTAAAGAA ATGAGTGACA GCTATGAAGG ATCCACCGGT
    GGAGGAAAAG GTGATGGCAA ACCCAGAAAG CATCACCGCA AATCTGCTCG CACACGTTCA
    CGCCAAGAAA AAATTAACAA GCCAAAACTA AGCATGTTAA ATGTTAGTAA TACGGGTGAC
    AAGATGGTGG AATGCCAGTT GGAGACACAC AATCATAAAA TGGTGACTTT CAAGTTCGAC
    CTAGATGGAG ATGCGCCTGA AGAAATAGCC ACTTATATGG TGGAAAATGG TTTCATTCTA
    CCAATAGAAA AGGAGATTTT CATAGATCAA CTAAAAGACA TTGTTGACAA GGCAGAGGAT
    ATTTTAAGTG AGGACATGGA TGGAGAGAAG ACATCAGAAC TTGGAAAAGG ACAGACACCT
    GGAGATAAAG GAAGAGAGGC AATAAATGGA CAGCAACCTG GGATTCCCCA GGCAGTTTAT
    CAGCAAAATG TTCTGCACAC AGGCAAGAGG TGGTTCATCA TCTGCCCTGT AGAAGAAGCT
    CCTGCAGCAA GTCAGGATGG ATGCTCAGAT GGAGGTCCAT CTACACAATC TCCATCTACA
    ACAACAATAA CTGAAGGAAC AGCCACTGCT CAGCCTGTAG AGAGTGCATC AGTACAACCA
    CCAGAACCCA GCACAGGATC TGCTTCCGCT TCTATGGATT CAGAAACCTG TGCCACAGCG
    CCTCCATCTG GAGGAAGTGA TCCCTATGGG GTCTGGAGCC CTCTCTCTTT GACAAATACT
    GATCCTCTTT CTCTGGCTGC TCTTTCCCAA GCTCCTCCTG CCCCGCAAGC AGCTATGATG
    CCAGCACAGC CTGCTTCACA TTCAGAGGAG GGCCAAAATT TGGGCTCTAC CCAGGTCAGT
    GTACAGCAGT CCCAGCCCTG CATAGATCCT CAAAGCTCTC TTTTTGTGGA AGAGACTCAA
    AGTGGCAGAT TAGACTCGGT CTCCGCAATG CACACCGCTC AGCAAATGGC TGAAATTGCA
    TGTGCTGCCT CATTGGTGGA GGACGTGCCC TGCTGTCCCT TGGTCATGCC GTTGTCCCTT
    GAAGTAAGCA GCGGGGCTCA GAAGCCATCA ACCGTAATTC CGCCACCATC ACAAGACAGT
    ACATCTGCTC GCGAACAACT CCAATCCATT ACATCTAATC GAGCGGAGCG TGGCCTGCAA
    CCTGTGGTGC TGCAGCAGCC TGTTTCCACT GTAAGTGGAT CTAAGGCTCC TTCCCTGCCT
    CAGAGCCCTG CTCCTTCACA GCACCACTTT GTCCCTGGTG AGTCAGATGG AGAGGGGCGT
    AGCAGAGGAG GGTTTGTAGA CAGCACTATC AAGACTCTGG ATGAGAAATT GAGGAATTTA
    TTGTACCAAG AGTACGCTCC GATGTACCCA TCGGGAAGTG CAGCTGAAAC TCCAGGATCT
    GGTACAGAGT ATATCCAGTC TCCACCAGGA CCTGAGAGTG CCATGGGAGG GTCAGGAACC
    AGCACACCTA GTCTTATAGG AGAGGGACGA TTCAGGGCAG GTGAACAGCT GCCACAGATC
    CCAGAGCGGG TAGACAGTTT AAGCGCTCTC AGCGACTCTG CAGTTGGAGT TACTGTGTCA
    AGGAGACATG TGATGCCACA CTCTACATCT TGTTCTGGAT CTAGAAATCG ATATAAGATG
    GTGCCAAGCT CCACTGACAT CTTGACAGGT CAAGGTCGAA AGCAGCGCAG CCTGAGCAGC
    ACGGCATCTC CAGCACACCC TGGTGGATTT TTGGGAGAAT GTACCATGTA CGAAGAGCCG
    ACTATGTCAG CTACTACAGT TGGCAGGTTC TCAGTGGTCA GCACAGAAGA CGAGGTCACG
    CGTAGAAAGT ACAGTAGCAG ATATTCAGCT CCACCTGACT TTTATCTGGA CGCTCCACCT
    CCACTGACCA AACGGGGGTC TTTGCCAAGG GCGCAGACCT CGACTTCAGC AGATGCCACA
    GTTTGCACAC GATTTATGTC ATCTGACTCT GGGGCAGAAA GCAGCCCTGC AAAAATGGCA
    CCTACAACCC CATCCCGGCA TGGCAGGTCT GAAAGGAGAG GAAGCGACCT CATGAAAAGG
    GCAGTGGCTT TTCTAAAGCG CTCTGGCCGC AGCAGTAGTG TGCAAAGCTC TGATTCACCA
    AGCCGGAAAG GAGGGGTGTA TGGATCCTAT GTCAGCAGTG ACAATGACTC AGAGATGGAG
    GACTCAGATA TTAAAAAGGA GCTTCAAAAT CTAAGAGAAA AGCATATGAA GGAGATAAGT
    GAATTGGAGG CCCACCACAG AGAAGAGATT GAGCTTCTCT ATGTCAGATT AGGAAAACCA
    CCACCTCCAG GTCTATACAT CCCACCCACA GTGCCCCCTG CTGGACGCAA GCGCAGGACC
    AGCAGGCACA AACTAAAAGC TGGCAAACTG CTCAGCCCTC TAGTACAACA ACTGAGAAAT
    GTTGCTTCTA AAACTGGTGA CTCCAGCAAA CCTAATGATT TAACCAAATC TGCTGAGGCG
    GTCCCGAGCT TGAATGGATC TCCTGTCAGA GCATCCAACT TGTCTGATGG CAGGCCTTAT
    TCTGGGACCG GGACTCTGCC TTGTTCTGTT TCTGAGCCAG TTCAGACCCA ACAGCCCTGC
    TCTCTTAAAG GATCTCTGTC TTCCGACAAT ATCTACTATG GTGCACAGGG AGAAGGACCC
    GTGCCACGCA GCCAGCCTGC CCAAGGCTGG CCTCCTTCTC CCCAGGCGTC TGCGCAGGTC
    ACCTATAAAT CCAGTAGTAA ACCACGCGCT AGATTCCTCA GGTCAACACT TAAGCGCCTG
    TGTCTGGGTA AAGAACGTGG CAGCAGATCT GGAGCATCAT CAGGTGCCTC AAATCAATCC
    CCTATGCCTC CTGGATCCAC TCCTCCTCCT CACCAGCCTG TTGGCCTTGC CCAAGCACAG
    ACAAACAACA GCAACAACAA AACAGATGCT TTCACTCAGC AGCTTCACCG ACTGGTGGAT
    AACTGGGCAG AAGGTGTGAG GGCTCCTTCA CGCTCACGCT CCCTCAGTCT AAGACCACAG
    CAACTGACCA GAACCAGGAT CTGGAGTGCA ACAGAGGTTC CAGGGTCAAT GGAAAGATTG
    AATGCTTCCC GGCTGCCTCT TTCATGGCCA CAGATTGATT TCCATGCCTC TGTGATGGTG
    ACTGACACTT CACAAGTGCT CCAGCACAGT TTTATGGTGT CCAGTAACCC TTATGAAAAG
    ATGCTTAACT CCACGCACTT TGACAAGGAC CTTTGGCCAG CAATGACTGC CAATCTTAAC
    CAAGAAGTCT TTGCTTTCTC TGCAGCGGAC TCTCCGTCCT GGACAGCACC TTCTTCCCCC
    AGCGAGGGTC CTAGTTCTAG AAATAGGACC ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53086
    Clone ID Related Accession (Same CDS sequence) XM_009306233.2
    Accession Version XM_009306233.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306233.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    ACTTTATCAA CTCTCCAGCC ACTAATGCAG GCATCCACAG ATGGTCAGTC TATTCCACAG
    CATCTATTAC AGTCAGCATC TTCAATGCTC AGTACCCTAC CTCCACAACC ACAACTAAAT
    ATTCAGACAC CCCTTCTCCA GCCTCTACAA ATTTCCACAA AGTTTCCTTC GACATATCCT
    CTTTTACCAG AAGGGGGCAC ATCTACAGGG ACAGAACCTA TATCTTTCTC CTCAGTCTCT
    TACTCTTCTC CTTATCCCAC AAATGCTCCT CCTGCATCAT CCCCCTACTA CTCACCAAGC
    CCCATCAGCG CTCCCCTTCC CATAATACCC ATGCAGAATG TACCTTGCAT ACTAGGATCA
    GGCACACCTG TATCCACCCC TCTCAATGTT CCCACCCCTA TACCTCTCCT GGCTATGGCC
    CTCTCCCCAC CCATGCCTCC ACCAGAGCAA CATCTGCAAC AGATGTTCTC CCCAATTCCA
    ACACCAGAAG GTGCCCTTTA CCAACCTCAA CCCCAACCAA CACAGCCCTC CCTTCCCCTT
    CCAAATCCAG CATCTCTCCC TCAGCAAGAC CCGTCTATTC CTGAACCTTT TAAAGAGGAA
    TACTCTCACC ATGAGGAATT GCAGATCCTG GCTCCATCTC ACACTGTTAT GTCACAGATT
    CAGTCTCCTC CACAGGACAG TAAGTCAGAA ACAAAGTTTA CCAATGTCCA AGCCTTTTCT
    GAGACAGTGG AGCATCCTCT GCAGTCTACT GGCATTCAGA GAGCTTATGC TGTTCCTGAG
    AGTACTGGCA CAGCTAGTGC AACTCAGCAA AGGGCAGAAA CTTCTGCAAT GCCCAAACTC
    CCAGCACCAG AGCCCTGCCC ATTTCAACAC AGCACTGAGG CACCTGCATC TATTCCTCTC
    CATGCTTTTG TGTGCGACAG CCTAAATCAA GATGCAGGAT CTAGTAAAGA AATGAGTGAC
    AGCTATGAAG GATCCACCGG TGGAGGAAAA GGTGATGGCA AACCCAGAAA GCATCACCGC
    AAATCTGCTC GCACACGTTC ACGCCAAGAA AAAATTAACA AGCCAAAACT AAGCATGTTA
    AATGTTAGTA ATACGGGTGA CAAGATGGTG GAATGCCAGT TGGAGACACA CAATCATAAA
    ATGGTGACTT TCAAGTTCGA CCTAGATGGA GATGCGCCTG AAGAAATAGC CACTTATATG
    GTGGAAAATG GTTTCATTCT ACCAATAGAA AAGGAGATTT TCATAGATCA ACTAAAAGAC
    ATTGTTGACA AGGCAGAGGA TATTTTAAGT GAGGACATGG ATGGAGAGAA GACATCAGAA
    CTTGGAAAAG GACAGACACC TGGAGATAAA GGAAGAGAGG CAATAAATGG ACAGCAACCT
    GGGATTCCCC AGGCAGTTTA TCAGCAAAAT GTTCTGCACA CAGGCAAGAG GTGGTTCATC
    ATCTGCCCTG TAGAAGAAGC TCCTGCAGCA AGTCAGGATG GATGCTCAGA TGGAGGTCCA
    TCTACACAAT CTCCATCTAC AACAACAATA ACTGAAGGAA CAGCCACTGC TCAGCCTGTA
    GAGAGTGCAT CAGTACAACC ACCAGAACCC AGCACAGGAT CTGCTTCCGC TTCTATGGAT
    TCAGAAACCT GTGCCACAGC GCCTCCATCT GGAGGAAGTG ATCCCTATGG GGTCTGGAGC
    CCTCTCTCTT TGACAAATAC TGATCCTCTT TCTCTGGCTG CTCTTTCCCA AGCTCCTCCT
    GCCCCGCAAG CAGCTATGAT GCCAGCACAG CCTGCTTCAC ATTCAGAGGA GGGCCAAAAT
    TTGGGCTCTA CCCAGGTCAG TGTACAGCAG TCCCAGCCCT GCATAGATCC TCAAAGCTCT
    CTTTTTGTGG AAGAGACTCA AAGTGGCAGA TTAGACTCGG TCTCCGCAAT GCACACCGCT
    CAGCAAATGG CTGAAATTGC ATGTGCTGCC TCATTGGTGG AGGACGTGCC CTGCTGTCCC
    TTGGTCATGC CGTTGTCCCT TGAAGTAAGC AGCGGGGCTC AGAAGCCATC AACCGTAATT
    CCGCCACCAT CACAAGACAG TACATCTGCT CGCGAACAAC TCCAATCCAT TACATCTAAT
    CGAGCGGAGC GTGGCCTGCA ACCTGTGGTG CTGCAGCAGC CTGTTTCCAC TGTAAGTGGA
    TCTAAGGCTC CTTCCCTGCC TCAGAGCCCT GCTCCTTCAC AGCACCACTT TGTCCCTGGT
    GAGTCAGATG GAGAGGGGCG TAGCAGAGGA GGGTTTGTAG ACAGCACTAT CAAGACTCTG
    GATGAGAAAT TGAGGAATTT ATTGTACCAA GAGTACGCTC CGATGTACCC ATCGGGAAGT
    GCAGCTGAAA CTCCAGGATC TGGTACAGAG TATATCCAGT CTCCACCAGG ACCTGAGAGT
    GCCATGGGAG GGTCAGGAAC CAGCACACCT AGTCTTATAG GAGAGGGACG ATTCAGGGCA
    GGTGAACAGC TGCCACAGAT CCCAGAGCGG GTAGACAGTT TAAGCGCTCT CAGCGACTCT
    GCAGTTGGAG TTACTGTGTC AAGGAGACAT GTGATGCCAC ACTCTACATC TTGTTCTGGA
    TCTAGAAATC GATATAAGAT GGTGCCAAGC TCCACTGACA TCTTGACAGG TCAAGGTCGA
    AAGCAGCGCA GCCTGAGCAG CACGGCATCT CCAGCACACC CTGGTGGATT TTTGGGAGAA
    TGTACCATGT ACGAAGAGCC GACTATGTCA GCTACTACAG TTGGCAGGTT CTCAGTGGTC
    AGCACAGAAG ACGAGGTCAC GCGTAGAAAG TACAGTAGCA GATATTCAGC TCCACCTGAC
    TTTTATCTGG ACGCTCCACC TCCACTGACC AAACGGGGGT CTTTGCCAAG GGCGCAGACC
    TCGACTTCAG CAGATGCCAC AGTTTGCACA CGATTTATGT CATCTGACTC TGGGGCAGAA
    AGCAGCCCTG CAAAAATGGC ACCTACAACC CCATCCCGGC ATGGCAGGTC TGAAAGGAGA
    GGAAGCGACC TCATGAAAAG GGCAGTGGCT TTTCTAAAGC GCTCTGGCCG CAGCAGTAGT
    GTGCAAAGCT CTGATTCACC AAGCCGGAAA GGAGGGGTGT ATGGATCCTA TGTCAGCAGT
    GACAATGACT CAGAGATGGA GGACTCAGAT ATTAAAAAGG AGCTTCAAAA TCTAAGAGAA
    AAGCATATGA AGGAGATAAG TGAATTGGAG GCCCACCACA GAGAAGAGAT TGAGCTTCTC
    TATGTCAGAT TAGGAAAACC ACCACCTCCA GGTCTATACA TCCCACCCAC AGTGCCCCCT
    GCTGGACGCA AGCGCAGGAC CAGCAGGCAC AAACTAAAAG CTGGCAAACT GCTCAGCCCT
    CTAGTACAAC AACTGAGAAA TGTTGCTTCT AAAACTGGTG ACTCCAGCAA ACCTAATGAT
    TTAACCAAAT CTGCTGAGGC GGTCCCGAGC TTGAATGGAT CTCCTGTCAG AGCATCCAAC
    TTGTCTGATG GCAGGCCTTA TTCTGGGACC GGGACTCTGC CTTGTTCTGT TTCTGAGCCA
    GTTCAGACCC AACAGCCCTG CTCTCTTAAA GGATCTCTGT CTTCCGACAA TATCTACTAT
    GGTGCACAGG GAGAAGGACC CGTGCCACGC AGCCAGCCTG CCCAAGGCTG GCCTCCTTCT
    CCCCAGGCGT CTGCGCAGGT CACCTATAAA TCCAGTAGTA AACCACGCGC TAGATTCCTC
    AGTGGGCCAG TTTCTCTGTC CATCTGGTCA ACACTTAAGC GCCTGTGTCT GGGTAAAGAA
    CGTGGCAGCA GATCTGGAGC ATCATCAGGT GCCTCAAATC AATCCCCTAT GCCTCCTGGA
    TCCACTCCTC CTCCTCACCA GCCTGTTGGC CTTGCCCAAG CACAGACAAA CAACAGCAAC
    AACAAAACAG ATGCTTTCAC TCAGCAGCTT CACCGACTGG TGGATAACTG GGCAGAAGGT
    GTGAGGGCTC CTTCACGCTC ACGCTCCCTC AGTCTAAGAC CACAGCAACT GACCAGAACC
    AGGATCTGGA GTGCAACAGA GGTTCCAGGG TCAATGGAAA GATTGAATGC TTCCCGGCTG
    CCTCTTTCAT GGCCACAGAT TGATTTCCAT GCCTCTGTGA TGGTGACTGA CACTTCACAA
    GTGCTCCAGC ACAGTTTTAT GGTGTCCAGT AACCCTTATG AAAAGATGCT TAACTCCACG
    CACTTTGACA AGGACCTTTG GCCAGCAATG ACTGCCAATC TTAACCAAGA AGTCTTTGCT
    TTCTCTGCAG CGGACTCTCC GTCCTGGACA GCACCTTCTT CCCCCAGCGA GGGTCCTAGT
    TCTAGAAATA GGACCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304508.1
    CDS711..8471
    Translation

    Target ORF information:

    RefSeq Version XM_009306233.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306233.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    ACTTTATCAA CTCTCCAGCC ACTAATGCAG GCATCCACAG ATGGTCAGTC TATTCCACAG
    CATCTATTAC AGTCAGCATC TTCAATGCTC AGTACCCTAC CTCCACAACC ACAACTAAAT
    ATTCAGACAC CCCTTCTCCA GCCTCTACAA ATTTCCACAA AGTTTCCTTC GACATATCCT
    CTTTTACCAG AAGGGGGCAC ATCTACAGGG ACAGAACCTA TATCTTTCTC CTCAGTCTCT
    TACTCTTCTC CTTATCCCAC AAATGCTCCT CCTGCATCAT CCCCCTACTA CTCACCAAGC
    CCCATCAGCG CTCCCCTTCC CATAATACCC ATGCAGAATG TACCTTGCAT ACTAGGATCA
    GGCACACCTG TATCCACCCC TCTCAATGTT CCCACCCCTA TACCTCTCCT GGCTATGGCC
    CTCTCCCCAC CCATGCCTCC ACCAGAGCAA CATCTGCAAC AGATGTTCTC CCCAATTCCA
    ACACCAGAAG GTGCCCTTTA CCAACCTCAA CCCCAACCAA CACAGCCCTC CCTTCCCCTT
    CCAAATCCAG CATCTCTCCC TCAGCAAGAC CCGTCTATTC CTGAACCTTT TAAAGAGGAA
    TACTCTCACC ATGAGGAATT GCAGATCCTG GCTCCATCTC ACACTGTTAT GTCACAGATT
    CAGTCTCCTC CACAGGACAG TAAGTCAGAA ACAAAGTTTA CCAATGTCCA AGCCTTTTCT
    GAGACAGTGG AGCATCCTCT GCAGTCTACT GGCATTCAGA GAGCTTATGC TGTTCCTGAG
    AGTACTGGCA CAGCTAGTGC AACTCAGCAA AGGGCAGAAA CTTCTGCAAT GCCCAAACTC
    CCAGCACCAG AGCCCTGCCC ATTTCAACAC AGCACTGAGG CACCTGCATC TATTCCTCTC
    CATGCTTTTG TGTGCGACAG CCTAAATCAA GATGCAGGAT CTAGTAAAGA AATGAGTGAC
    AGCTATGAAG GATCCACCGG TGGAGGAAAA GGTGATGGCA AACCCAGAAA GCATCACCGC
    AAATCTGCTC GCACACGTTC ACGCCAAGAA AAAATTAACA AGCCAAAACT AAGCATGTTA
    AATGTTAGTA ATACGGGTGA CAAGATGGTG GAATGCCAGT TGGAGACACA CAATCATAAA
    ATGGTGACTT TCAAGTTCGA CCTAGATGGA GATGCGCCTG AAGAAATAGC CACTTATATG
    GTGGAAAATG GTTTCATTCT ACCAATAGAA AAGGAGATTT TCATAGATCA ACTAAAAGAC
    ATTGTTGACA AGGCAGAGGA TATTTTAAGT GAGGACATGG ATGGAGAGAA GACATCAGAA
    CTTGGAAAAG GACAGACACC TGGAGATAAA GGAAGAGAGG CAATAAATGG ACAGCAACCT
    GGGATTCCCC AGGCAGTTTA TCAGCAAAAT GTTCTGCACA CAGGCAAGAG GTGGTTCATC
    ATCTGCCCTG TAGAAGAAGC TCCTGCAGCA AGTCAGGATG GATGCTCAGA TGGAGGTCCA
    TCTACACAAT CTCCATCTAC AACAACAATA ACTGAAGGAA CAGCCACTGC TCAGCCTGTA
    GAGAGTGCAT CAGTACAACC ACCAGAACCC AGCACAGGAT CTGCTTCCGC TTCTATGGAT
    TCAGAAACCT GTGCCACAGC GCCTCCATCT GGAGGAAGTG ATCCCTATGG GGTCTGGAGC
    CCTCTCTCTT TGACAAATAC TGATCCTCTT TCTCTGGCTG CTCTTTCCCA AGCTCCTCCT
    GCCCCGCAAG CAGCTATGAT GCCAGCACAG CCTGCTTCAC ATTCAGAGGA GGGCCAAAAT
    TTGGGCTCTA CCCAGGTCAG TGTACAGCAG TCCCAGCCCT GCATAGATCC TCAAAGCTCT
    CTTTTTGTGG AAGAGACTCA AAGTGGCAGA TTAGACTCGG TCTCCGCAAT GCACACCGCT
    CAGCAAATGG CTGAAATTGC ATGTGCTGCC TCATTGGTGG AGGACGTGCC CTGCTGTCCC
    TTGGTCATGC CGTTGTCCCT TGAAGTAAGC AGCGGGGCTC AGAAGCCATC AACCGTAATT
    CCGCCACCAT CACAAGACAG TACATCTGCT CGCGAACAAC TCCAATCCAT TACATCTAAT
    CGAGCGGAGC GTGGCCTGCA ACCTGTGGTG CTGCAGCAGC CTGTTTCCAC TGTAAGTGGA
    TCTAAGGCTC CTTCCCTGCC TCAGAGCCCT GCTCCTTCAC AGCACCACTT TGTCCCTGGT
    GAGTCAGATG GAGAGGGGCG TAGCAGAGGA GGGTTTGTAG ACAGCACTAT CAAGACTCTG
    GATGAGAAAT TGAGGAATTT ATTGTACCAA GAGTACGCTC CGATGTACCC ATCGGGAAGT
    GCAGCTGAAA CTCCAGGATC TGGTACAGAG TATATCCAGT CTCCACCAGG ACCTGAGAGT
    GCCATGGGAG GGTCAGGAAC CAGCACACCT AGTCTTATAG GAGAGGGACG ATTCAGGGCA
    GGTGAACAGC TGCCACAGAT CCCAGAGCGG GTAGACAGTT TAAGCGCTCT CAGCGACTCT
    GCAGTTGGAG TTACTGTGTC AAGGAGACAT GTGATGCCAC ACTCTACATC TTGTTCTGGA
    TCTAGAAATC GATATAAGAT GGTGCCAAGC TCCACTGACA TCTTGACAGG TCAAGGTCGA
    AAGCAGCGCA GCCTGAGCAG CACGGCATCT CCAGCACACC CTGGTGGATT TTTGGGAGAA
    TGTACCATGT ACGAAGAGCC GACTATGTCA GCTACTACAG TTGGCAGGTT CTCAGTGGTC
    AGCACAGAAG ACGAGGTCAC GCGTAGAAAG TACAGTAGCA GATATTCAGC TCCACCTGAC
    TTTTATCTGG ACGCTCCACC TCCACTGACC AAACGGGGGT CTTTGCCAAG GGCGCAGACC
    TCGACTTCAG CAGATGCCAC AGTTTGCACA CGATTTATGT CATCTGACTC TGGGGCAGAA
    AGCAGCCCTG CAAAAATGGC ACCTACAACC CCATCCCGGC ATGGCAGGTC TGAAAGGAGA
    GGAAGCGACC TCATGAAAAG GGCAGTGGCT TTTCTAAAGC GCTCTGGCCG CAGCAGTAGT
    GTGCAAAGCT CTGATTCACC AAGCCGGAAA GGAGGGGTGT ATGGATCCTA TGTCAGCAGT
    GACAATGACT CAGAGATGGA GGACTCAGAT ATTAAAAAGG AGCTTCAAAA TCTAAGAGAA
    AAGCATATGA AGGAGATAAG TGAATTGGAG GCCCACCACA GAGAAGAGAT TGAGCTTCTC
    TATGTCAGAT TAGGAAAACC ACCACCTCCA GGTCTATACA TCCCACCCAC AGTGCCCCCT
    GCTGGACGCA AGCGCAGGAC CAGCAGGCAC AAACTAAAAG CTGGCAAACT GCTCAGCCCT
    CTAGTACAAC AACTGAGAAA TGTTGCTTCT AAAACTGGTG ACTCCAGCAA ACCTAATGAT
    TTAACCAAAT CTGCTGAGGC GGTCCCGAGC TTGAATGGAT CTCCTGTCAG AGCATCCAAC
    TTGTCTGATG GCAGGCCTTA TTCTGGGACC GGGACTCTGC CTTGTTCTGT TTCTGAGCCA
    GTTCAGACCC AACAGCCCTG CTCTCTTAAA GGATCTCTGT CTTCCGACAA TATCTACTAT
    GGTGCACAGG GAGAAGGACC CGTGCCACGC AGCCAGCCTG CCCAAGGCTG GCCTCCTTCT
    CCCCAGGCGT CTGCGCAGGT CACCTATAAA TCCAGTAGTA AACCACGCGC TAGATTCCTC
    AGTGGGCCAG TTTCTCTGTC CATCTGGTCA ACACTTAAGC GCCTGTGTCT GGGTAAAGAA
    CGTGGCAGCA GATCTGGAGC ATCATCAGGT GCCTCAAATC AATCCCCTAT GCCTCCTGGA
    TCCACTCCTC CTCCTCACCA GCCTGTTGGC CTTGCCCAAG CACAGACAAA CAACAGCAAC
    AACAAAACAG ATGCTTTCAC TCAGCAGCTT CACCGACTGG TGGATAACTG GGCAGAAGGT
    GTGAGGGCTC CTTCACGCTC ACGCTCCCTC AGTCTAAGAC CACAGCAACT GACCAGAACC
    AGGATCTGGA GTGCAACAGA GGTTCCAGGG TCAATGGAAA GATTGAATGC TTCCCGGCTG
    CCTCTTTCAT GGCCACAGAT TGATTTCCAT GCCTCTGTGA TGGTGACTGA CACTTCACAA
    GTGCTCCAGC ACAGTTTTAT GGTGTCCAGT AACCCTTATG AAAAGATGCT TAACTCCACG
    CACTTTGACA AGGACCTTTG GCCAGCAATG ACTGCCAATC TTAACCAAGA AGTCTTTGCT
    TTCTCTGCAG CGGACTCTCC GTCCTGGACA GCACCTTCTT CCCCCAGCGA GGGTCCTAGT
    TCTAGAAATA GGACCATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53087
    Clone ID Related Accession (Same CDS sequence) XM_009306234.2
    Accession Version XM_009306234.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306234.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GTTTCCTTCG ACATATCCTC TTTTACCAGA AGGGGGCACA
    TCTACAGGGA CAGAACCTAT ATCTTTCTCC TCAGTCTCTT ACTCTTCTCC TTATCCCACA
    AATGCTCCTC CTGCATCATC CCCCTACTAC TCACCAAGCC CCATCAGCGC TCCCCTTCCC
    ATAATACCCA TGCAGAATGT ACCTTGCATA CTAGGATCAG GCACACCTGT ATCCACCCCT
    CTCAATGTTC CCACCCCTAT ACCTCTCCTG GCTATGGCCC TCTCCCCACC CATGCCTCCA
    CCAGAGCAAC ATCTGCAACA GATGTTCTCC CCAATTCCAA CACCAGAAGG TGCCCTTTAC
    CAACCTCAAC CCCAACCAAC ACAGCCCTCC CTTCCCCTTC CAAATCCAGC ATCTCTCCCT
    CAGCAAGACC CGTCTATTCC TGAACCTTTT AAAGAGGAAT ACTCTCACCA TGAGGAATTG
    CAGATCCTGG CTCCATCTCA CACTGTTATG TCACAGATTC AGTCTCCTCC ACAGGACAGT
    AAGTCAGAAA CAAAGTTTAC CAATGTCCAA GCCTTTTCTG AGACAGTGGA GCATCCTCTG
    CAGTCTACTG GCATTCAGAG AGCTTATGCT GTTCCTGAGA GTACTGGCAC AGCTAGTGCA
    ACTCAGCAAA GGGCAGAAAC TTCTGCAATG CCCAAACTCC CAGCACCAGA GCCCTGCCCA
    TTTCAACACA GCACTGAGGC ACCTGCATCT ATTCCTCTCC ATGCTTTTGT GTGCGACAGC
    CTAAATCAAG ATGCAGGATC TAGTAAAGAA ATGAGTGACA GCTATGAAGG ATCCACCGGT
    GGAGGAAAAG GTGATGGCAA ACCCAGAAAG CATCACCGCA AATCTGCTCG CACACGTTCA
    CGCCAAGAAA AAATTAACAA GCCAAAACTA AGCATGTTAA ATGTTAGTAA TACGGGTGAC
    AAGATGGTGG AATGCCAGTT GGAGACACAC AATCATAAAA TGGTGACTTT CAAGTTCGAC
    CTAGATGGAG ATGCGCCTGA AGAAATAGCC ACTTATATGG TGGAAAATGG TTTCATTCTA
    CCAATAGAAA AGGAGATTTT CATAGATCAA CTAAAAGACA TTGTTGACAA GGCAGAGGAT
    ATTTTAAGTG AGGACATGGA TGGAGAGAAG ACATCAGAAC TTGGAAAAGG ACAGACACCT
    GGAGATAAAG GAAGAGAGGC AATAAATGGA CAGCAACCTG GGATTCCCCA GGCAGTTTAT
    CAGCAAAATG TTCTGCACAC AGGCAAGAGG TGGTTCATCA TCTGCCCTGT AGAAGAAGCT
    CCTGCAGCAA GTCAGGATGG ATGCTCAGAT GGAGGTCCAT CTACACAATC TCCATCTACA
    ACAACAATAA CTGAAGGAAC AGCCACTGCT CAGCCTGTAG AGAGTGCATC AGTACAACCA
    CCAGAACCCA GCACAGGATC TGCTTCCGCT TCTATGGATT CAGAAACCTG TGCCACAGCG
    CCTCCATCTG GAGGAAGTGA TCCCTATGGG GTCTGGAGCC CTCTCTCTTT GACAAATACT
    GATCCTCTTT CTCTGGCTGC TCTTTCCCAA GCTCCTCCTG CCCCGCAAGC AGCTATGATG
    CCAGCACAGC CTGCTTCACA TTCAGAGGAG GGCCAAAATT TGGGCTCTAC CCAGGTCAGT
    GTACAGCAGT CCCAGCCCTG CATAGATCCT CAAAGCTCTC TTTTTGTGGA AGAGACTCAA
    AGTGGCAGAT TAGACTCGGT CTCCGCAATG CACACCGCTC AGCAAATGGC TGAAATTGCA
    TGTGCTGCCT CATTGGTGGA GGACGTGCCC TGCTGTCCCT TGGTCATGCC GTTGTCCCTT
    GAAGTAAGCA GCGGGGCTCA GAAGCCATCA ACCGTAATTC CGCCACCATC ACAAGACAGT
    ACATCTGCTC GCGAACAACT CCAATCCATT ACATCTAATC GAGCGGAGCG TGGCCTGCAA
    CCTGTGGTGC TGCAGCAGCC TGTTTCCACT GTAAGTGGAT CTAAGGCTCC TTCCCTGCCT
    CAGAGCCCTG CTCCTTCACA GCACCACTTT GTCCCTGGTG AGTCAGATGG AGAGGGGCGT
    AGCAGAGGAG GGTTTGTAGA CAGCACTATC AAGACTCTGG ATGAGAAATT GAGGAATTTA
    TTGTACCAAG AGTACGCTCC GATGTACCCA TCGGGAAGTG CAGCTGAAAC TCCAGGATCT
    GGTACAGAGT ATATCCAGTC TCCACCAGGA CCTGAGAGTG CCATGGGAGG GTCAGGAACC
    AGCACACCTA GTCTTATAGG AGAGGGACGA TTCAGGGCAG GTGAACAGCT GCCACAGATC
    CCAGAGCGGG TAGACAGTTT AAGCGCTCTC AGCGACTCTG CAGTTGGAGT TACTGTGTCA
    AGGAGACATG TGATGCCACA CTCTACATCT TGTTCTGGAT CTAGAAATCG ATATAAGATG
    GTGCCAAGCT CCACTGACAT CTTGACAGGT CAAGGTCGAA AGCAGCGCAG CCTGAGCAGC
    ACGGCATCTC CAGCACACCC TGGTGGATTT TTGGGAGAAT GTACCATGTA CGAAGAGCCG
    ACTATGTCAG CTACTACAGT TGGCAGGTTC TCAGTGGTCA GCACAGAAGA CGAGGTCACG
    CGTAGAAAGT ACAGTAGCAG ATATTCAGCT CCACCTGACT TTTATCTGGA CGCTCCACCT
    CCACTGACCA AACGGGGGTC TTTGCCAAGG GCGCAGACCT CGACTTCAGC AGATGCCACA
    GTTTGCACAC GATTTATGTC ATCTGACTCT GGGGCAGAAA GCAGCCCTGC AAAAATGGCA
    CCTACAACCC CATCCCGGCA TGGCAGGTCT GAAAGGAGAG GAAGCGACCT CATGAAAAGG
    GCAGTGGCTT TTCTAAAGCG CTCTGGCCGC AGCAGTAGTG TGCAAAGCTC TGATTCACCA
    AGCCGGAAAG GAGGGGTGTA TGGATCCTAT GTCAGCAGTG ACAATGACTC AGAGATGGAG
    GACTCAGATA TTAAAAAGGA GCTTCAAAAT CTAAGAGAAA AGCATATGAA GGAGATAAGT
    GAATTGGAGG CCCACCACAG AGAAGAGATT GAGCTTCTCT ATGTCAGATT AGGAAAACCA
    CCACCTCCAG GTCTATACAT CCCACCCACA GTGCCCCCTG CTGGACGCAA GCGCAGGACC
    AGCAGGCACA AACTAAAAGC TGGCAAACTG CTCAGCCCTC TAGTACAACA ACTGAGAAAT
    GTTGCTTCTA AAACTGGTGA CTCCAGCAAA CCTAATGATT TAACCAAATC TGCTGAGGCG
    GTCCCGAGCT TGAATGGATC TCCTGTCAGA GCATCCAACT TGTCTGATGG CAGGCCTTAT
    TCTGGGACCG GGACTCTGCC TTGTTCTGTT TCTGAGCCAG TTCAGACCCA ACAGCCCTGC
    TCTCTTAAAG GATCTCTGTC TTCCGACAAT ATCTACTATG GTGCACAGGG AGAAGGACCC
    GTGCCACGCA GCCAGCCTGC CCAAGGCTGG CCTCCTTCTC CCCAGGCGTC TGCGCAGGTC
    ACCTATAAAT CCAGTAGTAA ACCACGCGCT AGATTCCTCA GTGGGCCAGT TTCTCTGTCC
    ATCTGGTCAA CACTTAAGCG CCTGTGTCTG GGTAAAGAAC GTGGCAGCAG ATCTGGAGCA
    TCATCAGGTG CCTCAAATCA ATCCCCTATG CCTCCTGGAT CCACTCCTCC TCCTCACCAG
    CCTGTTGGCC TTGCCCAAGC ACAGACAAAC AACAGCAACA ACAAAACAGA TGCTTTCACT
    CAGCAGCTTC ACCGACTGGT GGATAACTGG GCAGAAGGTG TGAGGGCTCC TTCACGCTCA
    CGCTCCCTCA GTCTAAGACC ACAGCAACTG ACCAGAACCA GGATCTGGAG TGCAACAGAG
    AATGTCTATC AATCAAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304509.1
    CDS714..8234
    Translation

    Target ORF information:

    RefSeq Version XM_009306234.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306234.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GTTTCCTTCG ACATATCCTC TTTTACCAGA AGGGGGCACA
    TCTACAGGGA CAGAACCTAT ATCTTTCTCC TCAGTCTCTT ACTCTTCTCC TTATCCCACA
    AATGCTCCTC CTGCATCATC CCCCTACTAC TCACCAAGCC CCATCAGCGC TCCCCTTCCC
    ATAATACCCA TGCAGAATGT ACCTTGCATA CTAGGATCAG GCACACCTGT ATCCACCCCT
    CTCAATGTTC CCACCCCTAT ACCTCTCCTG GCTATGGCCC TCTCCCCACC CATGCCTCCA
    CCAGAGCAAC ATCTGCAACA GATGTTCTCC CCAATTCCAA CACCAGAAGG TGCCCTTTAC
    CAACCTCAAC CCCAACCAAC ACAGCCCTCC CTTCCCCTTC CAAATCCAGC ATCTCTCCCT
    CAGCAAGACC CGTCTATTCC TGAACCTTTT AAAGAGGAAT ACTCTCACCA TGAGGAATTG
    CAGATCCTGG CTCCATCTCA CACTGTTATG TCACAGATTC AGTCTCCTCC ACAGGACAGT
    AAGTCAGAAA CAAAGTTTAC CAATGTCCAA GCCTTTTCTG AGACAGTGGA GCATCCTCTG
    CAGTCTACTG GCATTCAGAG AGCTTATGCT GTTCCTGAGA GTACTGGCAC AGCTAGTGCA
    ACTCAGCAAA GGGCAGAAAC TTCTGCAATG CCCAAACTCC CAGCACCAGA GCCCTGCCCA
    TTTCAACACA GCACTGAGGC ACCTGCATCT ATTCCTCTCC ATGCTTTTGT GTGCGACAGC
    CTAAATCAAG ATGCAGGATC TAGTAAAGAA ATGAGTGACA GCTATGAAGG ATCCACCGGT
    GGAGGAAAAG GTGATGGCAA ACCCAGAAAG CATCACCGCA AATCTGCTCG CACACGTTCA
    CGCCAAGAAA AAATTAACAA GCCAAAACTA AGCATGTTAA ATGTTAGTAA TACGGGTGAC
    AAGATGGTGG AATGCCAGTT GGAGACACAC AATCATAAAA TGGTGACTTT CAAGTTCGAC
    CTAGATGGAG ATGCGCCTGA AGAAATAGCC ACTTATATGG TGGAAAATGG TTTCATTCTA
    CCAATAGAAA AGGAGATTTT CATAGATCAA CTAAAAGACA TTGTTGACAA GGCAGAGGAT
    ATTTTAAGTG AGGACATGGA TGGAGAGAAG ACATCAGAAC TTGGAAAAGG ACAGACACCT
    GGAGATAAAG GAAGAGAGGC AATAAATGGA CAGCAACCTG GGATTCCCCA GGCAGTTTAT
    CAGCAAAATG TTCTGCACAC AGGCAAGAGG TGGTTCATCA TCTGCCCTGT AGAAGAAGCT
    CCTGCAGCAA GTCAGGATGG ATGCTCAGAT GGAGGTCCAT CTACACAATC TCCATCTACA
    ACAACAATAA CTGAAGGAAC AGCCACTGCT CAGCCTGTAG AGAGTGCATC AGTACAACCA
    CCAGAACCCA GCACAGGATC TGCTTCCGCT TCTATGGATT CAGAAACCTG TGCCACAGCG
    CCTCCATCTG GAGGAAGTGA TCCCTATGGG GTCTGGAGCC CTCTCTCTTT GACAAATACT
    GATCCTCTTT CTCTGGCTGC TCTTTCCCAA GCTCCTCCTG CCCCGCAAGC AGCTATGATG
    CCAGCACAGC CTGCTTCACA TTCAGAGGAG GGCCAAAATT TGGGCTCTAC CCAGGTCAGT
    GTACAGCAGT CCCAGCCCTG CATAGATCCT CAAAGCTCTC TTTTTGTGGA AGAGACTCAA
    AGTGGCAGAT TAGACTCGGT CTCCGCAATG CACACCGCTC AGCAAATGGC TGAAATTGCA
    TGTGCTGCCT CATTGGTGGA GGACGTGCCC TGCTGTCCCT TGGTCATGCC GTTGTCCCTT
    GAAGTAAGCA GCGGGGCTCA GAAGCCATCA ACCGTAATTC CGCCACCATC ACAAGACAGT
    ACATCTGCTC GCGAACAACT CCAATCCATT ACATCTAATC GAGCGGAGCG TGGCCTGCAA
    CCTGTGGTGC TGCAGCAGCC TGTTTCCACT GTAAGTGGAT CTAAGGCTCC TTCCCTGCCT
    CAGAGCCCTG CTCCTTCACA GCACCACTTT GTCCCTGGTG AGTCAGATGG AGAGGGGCGT
    AGCAGAGGAG GGTTTGTAGA CAGCACTATC AAGACTCTGG ATGAGAAATT GAGGAATTTA
    TTGTACCAAG AGTACGCTCC GATGTACCCA TCGGGAAGTG CAGCTGAAAC TCCAGGATCT
    GGTACAGAGT ATATCCAGTC TCCACCAGGA CCTGAGAGTG CCATGGGAGG GTCAGGAACC
    AGCACACCTA GTCTTATAGG AGAGGGACGA TTCAGGGCAG GTGAACAGCT GCCACAGATC
    CCAGAGCGGG TAGACAGTTT AAGCGCTCTC AGCGACTCTG CAGTTGGAGT TACTGTGTCA
    AGGAGACATG TGATGCCACA CTCTACATCT TGTTCTGGAT CTAGAAATCG ATATAAGATG
    GTGCCAAGCT CCACTGACAT CTTGACAGGT CAAGGTCGAA AGCAGCGCAG CCTGAGCAGC
    ACGGCATCTC CAGCACACCC TGGTGGATTT TTGGGAGAAT GTACCATGTA CGAAGAGCCG
    ACTATGTCAG CTACTACAGT TGGCAGGTTC TCAGTGGTCA GCACAGAAGA CGAGGTCACG
    CGTAGAAAGT ACAGTAGCAG ATATTCAGCT CCACCTGACT TTTATCTGGA CGCTCCACCT
    CCACTGACCA AACGGGGGTC TTTGCCAAGG GCGCAGACCT CGACTTCAGC AGATGCCACA
    GTTTGCACAC GATTTATGTC ATCTGACTCT GGGGCAGAAA GCAGCCCTGC AAAAATGGCA
    CCTACAACCC CATCCCGGCA TGGCAGGTCT GAAAGGAGAG GAAGCGACCT CATGAAAAGG
    GCAGTGGCTT TTCTAAAGCG CTCTGGCCGC AGCAGTAGTG TGCAAAGCTC TGATTCACCA
    AGCCGGAAAG GAGGGGTGTA TGGATCCTAT GTCAGCAGTG ACAATGACTC AGAGATGGAG
    GACTCAGATA TTAAAAAGGA GCTTCAAAAT CTAAGAGAAA AGCATATGAA GGAGATAAGT
    GAATTGGAGG CCCACCACAG AGAAGAGATT GAGCTTCTCT ATGTCAGATT AGGAAAACCA
    CCACCTCCAG GTCTATACAT CCCACCCACA GTGCCCCCTG CTGGACGCAA GCGCAGGACC
    AGCAGGCACA AACTAAAAGC TGGCAAACTG CTCAGCCCTC TAGTACAACA ACTGAGAAAT
    GTTGCTTCTA AAACTGGTGA CTCCAGCAAA CCTAATGATT TAACCAAATC TGCTGAGGCG
    GTCCCGAGCT TGAATGGATC TCCTGTCAGA GCATCCAACT TGTCTGATGG CAGGCCTTAT
    TCTGGGACCG GGACTCTGCC TTGTTCTGTT TCTGAGCCAG TTCAGACCCA ACAGCCCTGC
    TCTCTTAAAG GATCTCTGTC TTCCGACAAT ATCTACTATG GTGCACAGGG AGAAGGACCC
    GTGCCACGCA GCCAGCCTGC CCAAGGCTGG CCTCCTTCTC CCCAGGCGTC TGCGCAGGTC
    ACCTATAAAT CCAGTAGTAA ACCACGCGCT AGATTCCTCA GTGGGCCAGT TTCTCTGTCC
    ATCTGGTCAA CACTTAAGCG CCTGTGTCTG GGTAAAGAAC GTGGCAGCAG ATCTGGAGCA
    TCATCAGGTG CCTCAAATCA ATCCCCTATG CCTCCTGGAT CCACTCCTCC TCCTCACCAG
    CCTGTTGGCC TTGCCCAAGC ACAGACAAAC AACAGCAACA ACAAAACAGA TGCTTTCACT
    CAGCAGCTTC ACCGACTGGT GGATAACTGG GCAGAAGGTG TGAGGGCTCC TTCACGCTCA
    CGCTCCCTCA GTCTAAGACC ACAGCAACTG ACCAGAACCA GGATCTGGAG TGCAACAGAG
    AATGTCTATC AATCAAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53088
    Clone ID Related Accession (Same CDS sequence) XM_009306235.2
    Accession Version XM_009306235.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306235.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GGAATACTCT CACCATGAGG AATTGCAGAT CCTGGCTCCA
    TCTCACACTG TTATGTCACA GATTCAGTCT CCTCCACAGG ACAGTAAGTC AGAAACAAAG
    TTTACCAATG TCCAAGCCTT TTCTGAGACA GTGGAGCATC CTCTGCAGTC TACTGGCATT
    CAGAGAGCTT ATGCTGTTCC TGAGAGTACT GGCACAGCTA GTGCAACTCA GCAAAGGGCA
    GAAACTTCTG CAATGCCCAA ACTCCCAGCA CCAGAGCCCT GCCCATTTCA ACACAGCACT
    GAGGCACCTG CATCTATTCC TCTCCATGCT TTTGTGTGCG ACAGCCTAAA TCAAGATGCA
    GGATCTAGTA AAGAAATGAG TGACAGCTAT GAAGGATCCA CCGGTGGAGG AAAAGGTGAT
    GGCAAACCCA GAAAGCATCA CCGCAAATCT GCTCGCACAC GTTCACGCCA AGAAAAAATT
    AACAAGCCAA AACTAAGCAT GTTAAATGTT AGTAATACGG GTGACAAGAT GGTGGAATGC
    CAGTTGGAGA CACACAATCA TAAAATGGTG ACTTTCAAGT TCGACCTAGA TGGAGATGCG
    CCTGAAGAAA TAGCCACTTA TATGGTGGAA AATGGTTTCA TTCTACCAAT AGAAAAGGAG
    ATTTTCATAG ATCAACTAAA AGACATTGTT GACAAGGCAG AGGATATTTT AAGTGAGGAC
    ATGGATGGAG AGAAGACATC AGAACTTGGA AAAGGACAGA CACCTGGAGA TAAAGGAAGA
    GAGGCAATAA ATGGACAGCA ACCTGGGATT CCCCAGGCAG TTTATCAGCA AAATGTTCTG
    CACACAGGCA AGAGGTGGTT CATCATCTGC CCTGTAGAAG AAGCTCCTGC AGCAAGTCAG
    GATGGATGCT CAGATGGAGG TCCATCTACA CAATCTCCAT CTACAACAAC AATAACTGAA
    GGAACAGCCA CTGCTCAGCC TGTAGAGAGT GCATCAGTAC AACCACCAGA ACCCAGCACA
    GGATCTGCTT CCGCTTCTAT GGATTCAGAA ACCTGTGCCA CAGCGCCTCC ATCTGGAGGA
    AGTGATCCCT ATGGGGTCTG GAGCCCTCTC TCTTTGACAA ATACTGATCC TCTTTCTCTG
    GCTGCTCTTT CCCAAGCTCC TCCTGCCCCG CAAGCAGCTA TGATGCCAGC ACAGCCTGCT
    TCACATTCAG AGGAGGGCCA AAATTTGGGC TCTACCCAGG TCAGTGTACA GCAGTCCCAG
    CCCTGCATAG ATCCTCAAAG CTCTCTTTTT GTGGAAGAGA CTCAAAGTGG CAGATTAGAC
    TCGGTCTCCG CAATGCACAC CGCTCAGCAA ATGGCTGAAA TTGCATGTGC TGCCTCATTG
    GTGGAGGACG TGCCCTGCTG TCCCTTGGTC ATGCCGTTGT CCCTTGAAGT AAGCAGCGGG
    GCTCAGAAGC CATCAACCGT AATTCCGCCA CCATCACAAG ACAGTACATC TGCTCGCGAA
    CAACTCCAAT CCATTACATC TAATCGAGCG GAGCGTGGCC TGCAACCTGT GGTGCTGCAG
    CAGCCTGTTT CCACTGTAAG TGGATCTAAG GCTCCTTCCC TGCCTCAGAG CCCTGCTCCT
    TCACAGCACC ACTTTGTCCC TGGTGAGTCA GATGGAGAGG GGCGTAGCAG AGGAGGGTTT
    GTAGACAGCA CTATCAAGAC TCTGGATGAG AAATTGAGGA ATTTATTGTA CCAAGAGTAC
    GCTCCGATGT ACCCATCGGG AAGTGCAGCT GAAACTCCAG GATCTGGTAC AGAGTATATC
    CAGTCTCCAC CAGGACCTGA GAGTGCCATG GGAGGGTCAG GAACCAGCAC ACCTAGTCTT
    ATAGGAGAGG GACGATTCAG GGCAGGTGAA CAGCTGCCAC AGATCCCAGA GCGGGTAGAC
    AGTTTAAGCG CTCTCAGCGA CTCTGCAGTT GGAGTTACTG TGTCAAGGAG ACATGTGATG
    CCACACTCTA CATCTTGTTC TGGATCTAGA AATCGATATA AGATGGTGCC AAGCTCCACT
    GACATCTTGA CAGGTCAAGG TCGAAAGCAG CGCAGCCTGA GCAGCACGGC ATCTCCAGCA
    CACCCTGGTG GATTTTTGGG AGAATGTACC ATGTACGAAG AGCCGACTAT GTCAGCTACT
    ACAGTTGGCA GGTTCTCAGT GGTCAGCACA GAAGACGAGG TCACGCGTAG AAAGTACAGT
    AGCAGATATT CAGCTCCACC TGACTTTTAT CTGGACGCTC CACCTCCACT GACCAAACGG
    GGGTCTTTGC CAAGGGCGCA GACCTCGACT TCAGCAGATG CCACAGTTTG CACACGATTT
    ATGTCATCTG ACTCTGGGGC AGAAAGCAGC CCTGCAAAAA TGGCACCTAC AACCCCATCC
    CGGCATGGCA GGTCTGAAAG GAGAGGAAGC GACCTCATGA AAAGGGCAGT GGCTTTTCTA
    AAGCGCTCTG GCCGCAGCAG TAGTGTGCAA AGCTCTGATT CACCAAGCCG GAAAGGAGGG
    GTGTATGGAT CCTATGTCAG CAGTGACAAT GACTCAGAGA TGGAGGACTC AGATATTAAA
    AAGGAGCTTC AAAATCTAAG AGAAAAGCAT ATGAAGGAGA TAAGTGAATT GGAGGCCCAC
    CACAGAGAAG AGATTGAGCT TCTCTATGTC AGATTAGGAA AACCACCACC TCCAGGTCTA
    TACATCCCAC CCACAGTGCC CCCTGCTGGA CGCAAGCGCA GGACCAGCAG GCACAAACTA
    AAAGCTGGCA AACTGCTCAG CCCTCTAGTA CAACAACTGA GAAATGTTGC TTCTAAAACT
    GGTGACTCCA GCAAACCTAA TGATTTAACC AAATCTGCTG AGGCGGTCCC GAGCTTGAAT
    GGATCTCCTG TCAGAGCATC CAACTTGTCT GATGGCAGGC CTTATTCTGG GACCGGGACT
    CTGCCTTGTT CTGTTTCTGA GCCAGTTCAG ACCCAACAGC CCTGCTCTCT TAAAGGATCT
    CTGTCTTCCG ACAATATCTA CTATGGTGCA CAGGGAGAAG GACCCGTGCC ACGCAGCCAG
    CCTGCCCAAG GCTGGCCTCC TTCTCCCCAG GCGTCTGCGC AGGTCACCTA TAAATCCAGT
    AGTAAACCAC GCGCTAGATT CCTCAGTGGG CCAGTTTCTC TGTCCATCTG GTCAACACTT
    AAGCGCCTGT GTCTGGGTAA AGAACGTGGC AGCAGATCTG GAGCATCATC AGGTGCCTCA
    AATCAATCCC CTATGCCTCC TGGATCCACT CCTCCTCCTC ACCAGCCTGT TGGCCTTGCC
    CAAGCACAGA CAAACAACAG CAACAACAAA ACAGATGCTT TCACTCAGCA GCTTCACCGA
    CTGGTGGATA ACTGGGCAGA AGGTGTGAGG GCTCCTTCAC GCTCACGCTC CCTCAGTCTA
    AGACCACAGC AACTGACCAG AACCAGGATC TGGAGTGCAA CAGAGGTTCC AGGGTCAATG
    GAAAGATTGA ATGCTTCCCG GCTGCCTCTT TCATGGCCAC AGATTGATTT CCATGCCTCT
    GTGATGGTGA CTGACACTTC ACAAGTGCTC CAGCACAGTT TTATGGTGTC CAGTAACCCT
    TATGAAAAGA TGCTTAACTC CACGCACTTT GACAAGGACC TTTGGCCAGC AATGACTGCC
    AATCTTAACC AAGAAGTCTT TGCTTTCTCT GCAGCGGACT CTCCGTCCTG GACAGCACCT
    TCTTCCCCCA GCGAGGGTCC TAGTTCTAGA AATAGGACCA TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304510.1
    CDS711..8075
    Translation

    Target ORF information:

    RefSeq Version XM_009306235.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306235.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GGAATACTCT CACCATGAGG AATTGCAGAT CCTGGCTCCA
    TCTCACACTG TTATGTCACA GATTCAGTCT CCTCCACAGG ACAGTAAGTC AGAAACAAAG
    TTTACCAATG TCCAAGCCTT TTCTGAGACA GTGGAGCATC CTCTGCAGTC TACTGGCATT
    CAGAGAGCTT ATGCTGTTCC TGAGAGTACT GGCACAGCTA GTGCAACTCA GCAAAGGGCA
    GAAACTTCTG CAATGCCCAA ACTCCCAGCA CCAGAGCCCT GCCCATTTCA ACACAGCACT
    GAGGCACCTG CATCTATTCC TCTCCATGCT TTTGTGTGCG ACAGCCTAAA TCAAGATGCA
    GGATCTAGTA AAGAAATGAG TGACAGCTAT GAAGGATCCA CCGGTGGAGG AAAAGGTGAT
    GGCAAACCCA GAAAGCATCA CCGCAAATCT GCTCGCACAC GTTCACGCCA AGAAAAAATT
    AACAAGCCAA AACTAAGCAT GTTAAATGTT AGTAATACGG GTGACAAGAT GGTGGAATGC
    CAGTTGGAGA CACACAATCA TAAAATGGTG ACTTTCAAGT TCGACCTAGA TGGAGATGCG
    CCTGAAGAAA TAGCCACTTA TATGGTGGAA AATGGTTTCA TTCTACCAAT AGAAAAGGAG
    ATTTTCATAG ATCAACTAAA AGACATTGTT GACAAGGCAG AGGATATTTT AAGTGAGGAC
    ATGGATGGAG AGAAGACATC AGAACTTGGA AAAGGACAGA CACCTGGAGA TAAAGGAAGA
    GAGGCAATAA ATGGACAGCA ACCTGGGATT CCCCAGGCAG TTTATCAGCA AAATGTTCTG
    CACACAGGCA AGAGGTGGTT CATCATCTGC CCTGTAGAAG AAGCTCCTGC AGCAAGTCAG
    GATGGATGCT CAGATGGAGG TCCATCTACA CAATCTCCAT CTACAACAAC AATAACTGAA
    GGAACAGCCA CTGCTCAGCC TGTAGAGAGT GCATCAGTAC AACCACCAGA ACCCAGCACA
    GGATCTGCTT CCGCTTCTAT GGATTCAGAA ACCTGTGCCA CAGCGCCTCC ATCTGGAGGA
    AGTGATCCCT ATGGGGTCTG GAGCCCTCTC TCTTTGACAA ATACTGATCC TCTTTCTCTG
    GCTGCTCTTT CCCAAGCTCC TCCTGCCCCG CAAGCAGCTA TGATGCCAGC ACAGCCTGCT
    TCACATTCAG AGGAGGGCCA AAATTTGGGC TCTACCCAGG TCAGTGTACA GCAGTCCCAG
    CCCTGCATAG ATCCTCAAAG CTCTCTTTTT GTGGAAGAGA CTCAAAGTGG CAGATTAGAC
    TCGGTCTCCG CAATGCACAC CGCTCAGCAA ATGGCTGAAA TTGCATGTGC TGCCTCATTG
    GTGGAGGACG TGCCCTGCTG TCCCTTGGTC ATGCCGTTGT CCCTTGAAGT AAGCAGCGGG
    GCTCAGAAGC CATCAACCGT AATTCCGCCA CCATCACAAG ACAGTACATC TGCTCGCGAA
    CAACTCCAAT CCATTACATC TAATCGAGCG GAGCGTGGCC TGCAACCTGT GGTGCTGCAG
    CAGCCTGTTT CCACTGTAAG TGGATCTAAG GCTCCTTCCC TGCCTCAGAG CCCTGCTCCT
    TCACAGCACC ACTTTGTCCC TGGTGAGTCA GATGGAGAGG GGCGTAGCAG AGGAGGGTTT
    GTAGACAGCA CTATCAAGAC TCTGGATGAG AAATTGAGGA ATTTATTGTA CCAAGAGTAC
    GCTCCGATGT ACCCATCGGG AAGTGCAGCT GAAACTCCAG GATCTGGTAC AGAGTATATC
    CAGTCTCCAC CAGGACCTGA GAGTGCCATG GGAGGGTCAG GAACCAGCAC ACCTAGTCTT
    ATAGGAGAGG GACGATTCAG GGCAGGTGAA CAGCTGCCAC AGATCCCAGA GCGGGTAGAC
    AGTTTAAGCG CTCTCAGCGA CTCTGCAGTT GGAGTTACTG TGTCAAGGAG ACATGTGATG
    CCACACTCTA CATCTTGTTC TGGATCTAGA AATCGATATA AGATGGTGCC AAGCTCCACT
    GACATCTTGA CAGGTCAAGG TCGAAAGCAG CGCAGCCTGA GCAGCACGGC ATCTCCAGCA
    CACCCTGGTG GATTTTTGGG AGAATGTACC ATGTACGAAG AGCCGACTAT GTCAGCTACT
    ACAGTTGGCA GGTTCTCAGT GGTCAGCACA GAAGACGAGG TCACGCGTAG AAAGTACAGT
    AGCAGATATT CAGCTCCACC TGACTTTTAT CTGGACGCTC CACCTCCACT GACCAAACGG
    GGGTCTTTGC CAAGGGCGCA GACCTCGACT TCAGCAGATG CCACAGTTTG CACACGATTT
    ATGTCATCTG ACTCTGGGGC AGAAAGCAGC CCTGCAAAAA TGGCACCTAC AACCCCATCC
    CGGCATGGCA GGTCTGAAAG GAGAGGAAGC GACCTCATGA AAAGGGCAGT GGCTTTTCTA
    AAGCGCTCTG GCCGCAGCAG TAGTGTGCAA AGCTCTGATT CACCAAGCCG GAAAGGAGGG
    GTGTATGGAT CCTATGTCAG CAGTGACAAT GACTCAGAGA TGGAGGACTC AGATATTAAA
    AAGGAGCTTC AAAATCTAAG AGAAAAGCAT ATGAAGGAGA TAAGTGAATT GGAGGCCCAC
    CACAGAGAAG AGATTGAGCT TCTCTATGTC AGATTAGGAA AACCACCACC TCCAGGTCTA
    TACATCCCAC CCACAGTGCC CCCTGCTGGA CGCAAGCGCA GGACCAGCAG GCACAAACTA
    AAAGCTGGCA AACTGCTCAG CCCTCTAGTA CAACAACTGA GAAATGTTGC TTCTAAAACT
    GGTGACTCCA GCAAACCTAA TGATTTAACC AAATCTGCTG AGGCGGTCCC GAGCTTGAAT
    GGATCTCCTG TCAGAGCATC CAACTTGTCT GATGGCAGGC CTTATTCTGG GACCGGGACT
    CTGCCTTGTT CTGTTTCTGA GCCAGTTCAG ACCCAACAGC CCTGCTCTCT TAAAGGATCT
    CTGTCTTCCG ACAATATCTA CTATGGTGCA CAGGGAGAAG GACCCGTGCC ACGCAGCCAG
    CCTGCCCAAG GCTGGCCTCC TTCTCCCCAG GCGTCTGCGC AGGTCACCTA TAAATCCAGT
    AGTAAACCAC GCGCTAGATT CCTCAGTGGG CCAGTTTCTC TGTCCATCTG GTCAACACTT
    AAGCGCCTGT GTCTGGGTAA AGAACGTGGC AGCAGATCTG GAGCATCATC AGGTGCCTCA
    AATCAATCCC CTATGCCTCC TGGATCCACT CCTCCTCCTC ACCAGCCTGT TGGCCTTGCC
    CAAGCACAGA CAAACAACAG CAACAACAAA ACAGATGCTT TCACTCAGCA GCTTCACCGA
    CTGGTGGATA ACTGGGCAGA AGGTGTGAGG GCTCCTTCAC GCTCACGCTC CCTCAGTCTA
    AGACCACAGC AACTGACCAG AACCAGGATC TGGAGTGCAA CAGAGGTTCC AGGGTCAATG
    GAAAGATTGA ATGCTTCCCG GCTGCCTCTT TCATGGCCAC AGATTGATTT CCATGCCTCT
    GTGATGGTGA CTGACACTTC ACAAGTGCTC CAGCACAGTT TTATGGTGTC CAGTAACCCT
    TATGAAAAGA TGCTTAACTC CACGCACTTT GACAAGGACC TTTGGCCAGC AATGACTGCC
    AATCTTAACC AAGAAGTCTT TGCTTTCTCT GCAGCGGACT CTCCGTCCTG GACAGCACCT
    TCTTCCCCCA GCGAGGGTCC TAGTTCTAGA AATAGGACCA TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53089
    Clone ID Related Accession (Same CDS sequence) XM_009306236.2
    Accession Version XM_009306236.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GTTTCCTTCG ACATATCCTC TTTTACCAGA AGGGGGCACA
    TCTACAGGGA CAGAACCTAT ATCTTTCTCC TCAGTCTCTT ACTCTTCTCC TTATCCCACA
    AATGCTCCTC CTGCATCATC CCCCTACTAC TCACCAAGCC CCATCAGCGC TCCCCTTCCC
    ATAATACCCA TGCAGAATGT ACCTTGCATA CTAGGATCAG GCACACCTGT ATCCACCCCT
    CTCAATGTTC CCACCCCTAT ACCTCTCCTG GCTATGGCCC TCTCCCCACC CATGCCTCCA
    CCAGAGCAAC ATCTGCAACA GATGTTCTCC CCAATTCCAA CACCAGAAGG TGCCCTTTAC
    CAACCTCAAC CCCAACCAAC ACAGCCCTCC CTTCCCCTTC CAAATCCAGC ATCTCTCCCT
    CAGCAAGACC CGTCTATTCC TGAACCTTTT AAAGAGGAAT ACTCTCACCA TGAGGAATTG
    CAGATCCTGG CTCCATCTCA CACTGTTATG TCACAGATTC AGTCTCCTCC ACAGGACAGT
    AAGTCAGAAA CAAAGTTTAC CAATGTCCAA GCCTTTTCTG AGACAGTGGA GCATCCTCTG
    CAGTCTACTG GCATTCAGAG AGCTTATGCT GTTCCTGAGA GTACTGGCAC AGCTAGTGCA
    ACTCAGCAAA GGGCAGAAAC TTCTGCAATG CCCAAACTCC CAGCACCAGA GCCCTGCCCA
    TTTCAACACA GCACTGAGGC ACCTGCATCT ATTCCTCTCC ATGCTTTTGT GTGCGACAGC
    CTAAATCAAG ATGCAGGATC TAGTAAAGAA ATGAGTGACA GCTATGAAGG ATCCACCGGT
    GGAGGAAAAG GTGATGGCAA ACCCAGAAAG CATCACCGCA AATCTGCTCG CACACGTTCA
    CGCCAAGAAA AAATTAACAA GCCAAAACTA AGCATGTTAA ATGTTAGTAA TACGGGTGAC
    AAGATGGTGG AATGCCAGTT GGAGACACAC AATCATAAAA TGGTGACTTT CAAGTTCGAC
    CTAGATGGAG ATGCGCCTGA AGAAATAGCC ACTTATATGG TGGAAAATGG TTTCATTCTA
    CCAATAGAAA AGGAGATTTT CATAGATCAA CTAAAAGACA TTGTTGACAA GGCAGAGGAT
    ATTTTAAGTG AGGACATGGA TGGAGAGAAG ACATCAGAAC TTGGAAAAGG ACAGACACCT
    GGAGATAAAG GAAGAGAGGC AATAAATGGA CAGCAACCTG GGATTCCCCA GGCAGTTTAT
    CAGCAAAATG TTCTGCACAC AGGCAAGAGG TGGTTCATCA TCTGCCCTGT AGAAGAAGCT
    CCTGCAGCAA GTCAGGATGG ATGCTCAGAT GGAGGTCCAT CTACACAATC TCCATCTACA
    ACAACAATAA CTGAAGGAAC AGCCACTGCT CAGCCTGTAG AGAGTGCATC AGTACAACCA
    CCAGAACCCA GCACAGGATC TGCTTCCGCT TCTATGGATT CAGAAACCTG TGCCACAGCG
    CCTCCATCTG GAGGAAGTGA TCCCTATGGG GTCTGGAGCC CTCTCTCTTT GACAAATACT
    GATCCTCTTT CTCTGGCTGC TCTTTCCCAA GCTCCTCCTG CCCCGCAAGC AGCTATGATG
    CCAGCACAGC CTGCTTCACA TTCAGAGGAG GGCCAAAATT TGGGCTCTAC CCAGGTCAGT
    GTACAGCAGT CCCAGCCCTG CATAGATCCT CAAAGCTCTC TTTTTGTGGA AGAGACTCAA
    AGTGGCAGAT TAGACTCGGT CTCCGCAATG CACACCGCTC AGCAAATGGC TGAAATTGCA
    TGTGCTGCCT CATTGGTGGA GGACGTGCCC TGCTGTCCCT TGGTCATGCC GTTGTCCCTT
    GAAGTAAGCA GCGGGGCTCA GAAGCCATCA ACCGTAATTC CGCCACCATC ACAAGACAGT
    ACATCTGCTC GCGAACAACT CCAATCCATT ACATCTAATC GAGCGGAGCG TGGCCTGCAA
    CCTGTGGTGC TGCAGCAGCC TGTTTCCACT GTAAGTGGAT CTAAGGCTCC TTCCCTGCCT
    CAGAGCCCTG CTCCTTCACA GCACCACTTT GTCCCTGGTG AGTCAGATGG AGAGGGGCGT
    AGCAGAGGAG GGTTTGTAGA CAGCACTATC AAGACTCTGG ATGAGAAATT GAGGAATTTA
    TTGTACCAAG AGTACGCTCC GATGTACCCA TCGGGAAGTG CAGCTGAAAC TCCAGGATCT
    GGTACAGAGT ATATCCAGTC TCCACCAGGA CCTGAGAGTG CCATGGGAGG GTCAGGAACC
    AGCACACCTA GTCTTATAGG AGAGGGACGA TTCAGGGCAG GTGAACAGCT GCCACAGATC
    CCAGAGCGGG TAGACAGTTT AAGCGCTCTC AGCGACTCTG CAGTTGGAGT TACTGTGTCA
    AGGAGACATG TGATGCCACA CTCTACATCT TGTTCTGGAT CTAGAAATCG ATATAAGATG
    GTGCCAAGCT CCACTGACAT CTTGACAGGT CAAGGTCGAA AGCAGCGCAG CCTGAGCAGC
    ACGGCATCTC CAGCACACCC TGGTGGATTT TTGGGAGAAT GTACCATGTA CGAAGAGCCG
    ACTATGTCAG CTACTACAGT TGGCAGGTTC TCAGTGGTCA GCACAGAAGA CGAGGTCACG
    CGTAGAAAGT ACAGTAGCAG ATATTCAGCT CCACCTGACT TTTATCTGGA CGCTCCACCT
    CCACTGACCA AACGGGGGTC TTTGCCAAGG GCGCAGACCT CGACTTCAGC AGATGCCACA
    GTTTGCACAC GATTTATGTC ATCTGACTCT GGGGCAGAAA GCAGCCCTGC AAAAATGGCA
    CCTACAACCC CATCCCGGCA TGGCAGGTCT GAAAGGAGAG GAAGCGACCT CATGAAAAGG
    GCAGTGGCTT TTCTAAAGCG CTCTGGCCGC AGCAGTAGTG TGCAAAGCTC TGATTCACCA
    AGCCGGAAAG GAGGGGTGTA TGGATCCTAT GTCAGCAGTG ACAATGACTC AGAGATGGAG
    GACTCAGATA TTAAAAAGGA GCTTCAAAAT CTAAGAGAAA AGCATATGAA GGAGATAAGT
    GAATTGGAGG CCCACCACAG AGAAGAGATT GAGCTTCTCT ATGTCAGATT AGGAAAACCA
    CCACCTCCAG GTCTATACAT CCCACCCACA GTGCCCCCTG CTGGACGCAA GCGCAGGACC
    AGCAGGCACA AACTAAAAGC TGGCAAACTG CTCAGCCCTC TAGTACAACA ACTGAGAAAT
    GTTGCTTCTA AAACTGGTGA CTCCAGCAAA CCTAATGATT TAACCAAATC TGCTGAGGCG
    GTCCCGAGCT TGAATGGATC TCCTGTCAGA GCATCCAACT TGTCTGATGG CAGGCCTTAT
    TCTGGGACCG GGACTCTGCC TTGTTCTGTT TCTGAGCCAG TTCAGACCCA ACAGCCCTGC
    TCTCTTAAAG GATCTCTGTC TTCCGACAAT ATCTACTATG GTGCACAGGG AGAAGGACCC
    GTGCCACGCA GCCAGCCTGC CCAAGGCTGG CCTCCTTCTC CCCAGGCGTC TGCGCAGGTC
    ACCTATAAAT CCAGTAGTAA ACCACGCGCT AGATTCCTCA GTGGGCCAGT TTCTCTGTCC
    ATCTGGTCAA CACTTAAGCG CCTGTGTCTG GGTAAAGAAC GTGGCAGCAT TCATTCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304511.1
    CDS716..7975
    Translation

    Target ORF information:

    RefSeq Version XM_009306236.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306236.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GTTTCCTTCG ACATATCCTC TTTTACCAGA AGGGGGCACA
    TCTACAGGGA CAGAACCTAT ATCTTTCTCC TCAGTCTCTT ACTCTTCTCC TTATCCCACA
    AATGCTCCTC CTGCATCATC CCCCTACTAC TCACCAAGCC CCATCAGCGC TCCCCTTCCC
    ATAATACCCA TGCAGAATGT ACCTTGCATA CTAGGATCAG GCACACCTGT ATCCACCCCT
    CTCAATGTTC CCACCCCTAT ACCTCTCCTG GCTATGGCCC TCTCCCCACC CATGCCTCCA
    CCAGAGCAAC ATCTGCAACA GATGTTCTCC CCAATTCCAA CACCAGAAGG TGCCCTTTAC
    CAACCTCAAC CCCAACCAAC ACAGCCCTCC CTTCCCCTTC CAAATCCAGC ATCTCTCCCT
    CAGCAAGACC CGTCTATTCC TGAACCTTTT AAAGAGGAAT ACTCTCACCA TGAGGAATTG
    CAGATCCTGG CTCCATCTCA CACTGTTATG TCACAGATTC AGTCTCCTCC ACAGGACAGT
    AAGTCAGAAA CAAAGTTTAC CAATGTCCAA GCCTTTTCTG AGACAGTGGA GCATCCTCTG
    CAGTCTACTG GCATTCAGAG AGCTTATGCT GTTCCTGAGA GTACTGGCAC AGCTAGTGCA
    ACTCAGCAAA GGGCAGAAAC TTCTGCAATG CCCAAACTCC CAGCACCAGA GCCCTGCCCA
    TTTCAACACA GCACTGAGGC ACCTGCATCT ATTCCTCTCC ATGCTTTTGT GTGCGACAGC
    CTAAATCAAG ATGCAGGATC TAGTAAAGAA ATGAGTGACA GCTATGAAGG ATCCACCGGT
    GGAGGAAAAG GTGATGGCAA ACCCAGAAAG CATCACCGCA AATCTGCTCG CACACGTTCA
    CGCCAAGAAA AAATTAACAA GCCAAAACTA AGCATGTTAA ATGTTAGTAA TACGGGTGAC
    AAGATGGTGG AATGCCAGTT GGAGACACAC AATCATAAAA TGGTGACTTT CAAGTTCGAC
    CTAGATGGAG ATGCGCCTGA AGAAATAGCC ACTTATATGG TGGAAAATGG TTTCATTCTA
    CCAATAGAAA AGGAGATTTT CATAGATCAA CTAAAAGACA TTGTTGACAA GGCAGAGGAT
    ATTTTAAGTG AGGACATGGA TGGAGAGAAG ACATCAGAAC TTGGAAAAGG ACAGACACCT
    GGAGATAAAG GAAGAGAGGC AATAAATGGA CAGCAACCTG GGATTCCCCA GGCAGTTTAT
    CAGCAAAATG TTCTGCACAC AGGCAAGAGG TGGTTCATCA TCTGCCCTGT AGAAGAAGCT
    CCTGCAGCAA GTCAGGATGG ATGCTCAGAT GGAGGTCCAT CTACACAATC TCCATCTACA
    ACAACAATAA CTGAAGGAAC AGCCACTGCT CAGCCTGTAG AGAGTGCATC AGTACAACCA
    CCAGAACCCA GCACAGGATC TGCTTCCGCT TCTATGGATT CAGAAACCTG TGCCACAGCG
    CCTCCATCTG GAGGAAGTGA TCCCTATGGG GTCTGGAGCC CTCTCTCTTT GACAAATACT
    GATCCTCTTT CTCTGGCTGC TCTTTCCCAA GCTCCTCCTG CCCCGCAAGC AGCTATGATG
    CCAGCACAGC CTGCTTCACA TTCAGAGGAG GGCCAAAATT TGGGCTCTAC CCAGGTCAGT
    GTACAGCAGT CCCAGCCCTG CATAGATCCT CAAAGCTCTC TTTTTGTGGA AGAGACTCAA
    AGTGGCAGAT TAGACTCGGT CTCCGCAATG CACACCGCTC AGCAAATGGC TGAAATTGCA
    TGTGCTGCCT CATTGGTGGA GGACGTGCCC TGCTGTCCCT TGGTCATGCC GTTGTCCCTT
    GAAGTAAGCA GCGGGGCTCA GAAGCCATCA ACCGTAATTC CGCCACCATC ACAAGACAGT
    ACATCTGCTC GCGAACAACT CCAATCCATT ACATCTAATC GAGCGGAGCG TGGCCTGCAA
    CCTGTGGTGC TGCAGCAGCC TGTTTCCACT GTAAGTGGAT CTAAGGCTCC TTCCCTGCCT
    CAGAGCCCTG CTCCTTCACA GCACCACTTT GTCCCTGGTG AGTCAGATGG AGAGGGGCGT
    AGCAGAGGAG GGTTTGTAGA CAGCACTATC AAGACTCTGG ATGAGAAATT GAGGAATTTA
    TTGTACCAAG AGTACGCTCC GATGTACCCA TCGGGAAGTG CAGCTGAAAC TCCAGGATCT
    GGTACAGAGT ATATCCAGTC TCCACCAGGA CCTGAGAGTG CCATGGGAGG GTCAGGAACC
    AGCACACCTA GTCTTATAGG AGAGGGACGA TTCAGGGCAG GTGAACAGCT GCCACAGATC
    CCAGAGCGGG TAGACAGTTT AAGCGCTCTC AGCGACTCTG CAGTTGGAGT TACTGTGTCA
    AGGAGACATG TGATGCCACA CTCTACATCT TGTTCTGGAT CTAGAAATCG ATATAAGATG
    GTGCCAAGCT CCACTGACAT CTTGACAGGT CAAGGTCGAA AGCAGCGCAG CCTGAGCAGC
    ACGGCATCTC CAGCACACCC TGGTGGATTT TTGGGAGAAT GTACCATGTA CGAAGAGCCG
    ACTATGTCAG CTACTACAGT TGGCAGGTTC TCAGTGGTCA GCACAGAAGA CGAGGTCACG
    CGTAGAAAGT ACAGTAGCAG ATATTCAGCT CCACCTGACT TTTATCTGGA CGCTCCACCT
    CCACTGACCA AACGGGGGTC TTTGCCAAGG GCGCAGACCT CGACTTCAGC AGATGCCACA
    GTTTGCACAC GATTTATGTC ATCTGACTCT GGGGCAGAAA GCAGCCCTGC AAAAATGGCA
    CCTACAACCC CATCCCGGCA TGGCAGGTCT GAAAGGAGAG GAAGCGACCT CATGAAAAGG
    GCAGTGGCTT TTCTAAAGCG CTCTGGCCGC AGCAGTAGTG TGCAAAGCTC TGATTCACCA
    AGCCGGAAAG GAGGGGTGTA TGGATCCTAT GTCAGCAGTG ACAATGACTC AGAGATGGAG
    GACTCAGATA TTAAAAAGGA GCTTCAAAAT CTAAGAGAAA AGCATATGAA GGAGATAAGT
    GAATTGGAGG CCCACCACAG AGAAGAGATT GAGCTTCTCT ATGTCAGATT AGGAAAACCA
    CCACCTCCAG GTCTATACAT CCCACCCACA GTGCCCCCTG CTGGACGCAA GCGCAGGACC
    AGCAGGCACA AACTAAAAGC TGGCAAACTG CTCAGCCCTC TAGTACAACA ACTGAGAAAT
    GTTGCTTCTA AAACTGGTGA CTCCAGCAAA CCTAATGATT TAACCAAATC TGCTGAGGCG
    GTCCCGAGCT TGAATGGATC TCCTGTCAGA GCATCCAACT TGTCTGATGG CAGGCCTTAT
    TCTGGGACCG GGACTCTGCC TTGTTCTGTT TCTGAGCCAG TTCAGACCCA ACAGCCCTGC
    TCTCTTAAAG GATCTCTGTC TTCCGACAAT ATCTACTATG GTGCACAGGG AGAAGGACCC
    GTGCCACGCA GCCAGCCTGC CCAAGGCTGG CCTCCTTCTC CCCAGGCGTC TGCGCAGGTC
    ACCTATAAAT CCAGTAGTAA ACCACGCGCT AGATTCCTCA GTGGGCCAGT TTCTCTGTCC
    ATCTGGTCAA CACTTAAGCG CCTGTGTCTG GGTAAAGAAC GTGGCAGCAT TCATTCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53090
    Clone ID Related Accession (Same CDS sequence) XM_009306237.2
    Accession Version XM_009306237.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306237.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GTTTCCTTCG ACATATCCTC TTTTACCAGA AGGGGGCACA
    TCTACAGGGA CAGAACCTAT ATCTTTCTCC TCAGTCTCTT ACTCTTCTCC TTATCCCACA
    AATGCTCCTC CTGCATCATC CCCCTACTAC TCACCAAGCC CCATCAGCGC TCCCCTTCCC
    ATAATACCCA TGCAGAATGT ACCTTGCATA CTAGGATCAG GCACACCTGT ATCCACCCCT
    CTCAATGTTC CCACCCCTAT ACCTCTCCTG GCTATGGCCC TCTCCCCACC CATGCCTCCA
    CCAGAGCAAC ATCTGCAACA GATGTTCTCC CCAATTCCAA CACCAGAAGG TGCCCTTTAC
    CAACCTCAAC CCCAACCAAC ACAGCCCTCC CTTCCCCTTC CAAATCCAGC ATCTCTCCCT
    CAGCAAGACC CGTCTATTCC TGAACCTTTT AAAGAGGAAT ACTCTCACCA TGAGGAATTG
    CAGATCCTGG CTCCATCTCA CACTGTTATG TCACAGATTC AGTCTCCTCC ACAGGACAGT
    AAGTCAGAAA CAAAGTTTAC CAATGTCCAA GCCTTTTCTG AGACAGTGGA GCATCCTCTG
    CAGTCTACTG GCATTCAGAG AGCTTATGCT GTTCCTGAGA GTACTGGCAC AGCTAGTGCA
    ACTCAGCAAA GGGCAGAAAC TTCTGCAATG CCCAAACTCC CAGCACCAGA GCCCTGCCCA
    TTTCAACACA GCACTGAGGC ACCTGCATCT ATTCCTCTCC ATGCTTTTGT GTGCGACAGC
    CTAAATCAAG ATGCAGGATC TAGTAAAGAA ATGAGTGACA GCTATGAAGG ATCCACCGGT
    GGAGGAAAAG GTGATGGCAA ACCCAGAAAG CATCACCGCA AATCTGCTCG CACACGTTCA
    CGCCAAGAAA AAATTAACAA GCCAAAACTA AGCATGTTAA ATGTTAGTAA TACGGGTGAC
    AAGATGGTGG AATGCCAGTT GGAGACACAC AATCATAAAA TGGTGACTTT CAAGTTCGAC
    CTAGATGGAG ATGCGCCTGA AGAAATAGCC ACTTATATGG TGGAAAATGG TTTCATTCTA
    CCAATAGAAA AGGAGATTTT CATAGATCAA CTAAAAGACA TTGTTGACAA GGCAGAGGAT
    ATTTTAAGTG AGGACATGGA TGGAGAGAAG ACATCAGAAC TTGGAAAAGG ACAGACACCT
    GGAGATAAAG GAAGAGAGGC AATAAATGGA CAGCAACCTG GGATTCCCCA GGCAGTTTAT
    CAGCAAAATG TTCTGCACAC AGGCAAGAGG TGGTTCATCA TCTGCCCTGT AGAAGAAGCT
    CCTGCAGCAA GTCAGGATGG ATGCTCAGAT GGAGGTCCAT CTACACAATC TCCATCTACA
    ACAACAATAA CTGAAGGAAC AGCCACTGCT CAGCCTGTAG AGAGTGCATC AGTACAACCA
    CCAGAACCCA GCACAGGATC TGCTTCCGCT TCTATGGATT CAGAAACCTG TGCCACAGCG
    CCTCCATCTG GAGGAAGTGA TCCCTATGGG GTCTGGAGCC CTCTCTCTTT GACAAATACT
    GATCCTCTTT CTCTGGCTGC TCTTTCCCAA GCTCCTCCTG CCCCGCAAGC AGCTATGATG
    CCAGCACAGC CTGCTTCACA TTCAGAGGAG GGCCAAAATT TGGGCTCTAC CCAGGTCAGT
    GTACAGCAGT CCCAGCCCTG CATAGATCCT CAAAGCTCTC TTTTTGTGGA AGAGACTCAA
    AGTGGCAGAT TAGACTCGGT CTCCGCAATG CACACCGCTC AGCAAATGGC TGAAATTGCA
    TGTGCTGCCT CATTGGTGGA GGACGTGCCC TGCTGTCCCT TGGTCATGCC GTTGTCCCTT
    GAAGTAAGCA GCGGGGCTCA GAAGCCATCA ACCGTAATTC CGCCACCATC ACAAGACAGT
    ACATCTGCTC GCGAACAACT CCAATCCATT ACATCTAATC GAGCGGAGCG TGGCCTGCAA
    CCTGTGGTGC TGCAGCAGCC TGTTTCCACT GTAAGTGGAT CTAAGGCTCC TTCCCTGCCT
    CAGAGCCCTG CTCCTTCACA GCACCACTTT GTCCCTGGTG AGTCAGATGG AGAGGGGCGT
    AGCAGAGGAG GGTTTGTAGA CAGCACTATC AAGACTCTGG ATGAGAAATT GAGGAATTTA
    TTGTACCAAG AGTACGCTCC GATGTACCCA TCGGGAAGTG CAGCTGAAAC TCCAGGATCT
    GGTACAGAGT ATATCCAGTC TCCACCAGGA CCTGAGAGTG CCATGGGAGG GTCAGGAACC
    AGCACACCTA GTCTTATAGG AGAGGGACGA TTCAGGGCAG GTGAACAGCT GCCACAGATC
    CCAGAGCGGG TAGACAGTTT AAGCGCTCTC AGCGACTCTG CAGTTGGAGT TACTGTGTCA
    AGGAGACATG TGATGCCACA CTCTACATCT TGTTCTGGAT CTAGAAATCG ATATAAGATG
    GTGCCAAGCT CCACTGACAT CTTGACAGGT CAAGGTCGAA AGCAGCGCAG CCTGAGCAGC
    ACGGCATCTC CAGCACACCC TGGTGGATTT TTGGGAGAAT GTACCATGTA CGAAGAGCCG
    ACTATGTCAG CTACTACAGT TGGCAGGTTC TCAGTGGTCA GCACAGAAGA CGAGGTCACG
    CGTAGAAAGT ACAGTAGCAG ATATTCAGCT CCACCTGACT TTTATCTGGA CGCTCCACCT
    CCACTGACCA AACGGGGGTC TTTGCCAAGG GCGCAGACCT CGACTTCAGC AGATGCCACA
    GTTTGCACAC GATTTATGTC ATCTGACTCT GGGGCAGAAA GCAGCCCTGC AAAAATGGCA
    CCTACAACCC CATCCCGGCA TGGCAGGTCT GAAAGGAGAG GAAGCGACCT CATGAAAAGG
    GCAGTGGCTT TTCTAAAGCG CTCTGGCCGC AGCAGTAGTG TGCAAAGCTC TGATTCACCA
    AGCCGGAAAG GAGGGGTGTA TGGATCCTAT GTCAGCAGTG ACAATGACTC AGAGATGGAG
    GACTCAGATA TTAAAAAGGA GCTTCAAAAT CTAAGAGAAA AGCATATGAA GGAGATAAGT
    GAATTGGAGG CCCACCACAG AGAAGAGATT GAGCTTCTCT ATGTCAGATT AGGAAAACCA
    CCACCTCCAG GTCTATACAT CCCACCCACA GTGCCCCCTG CTGGACGCAA GCGCAGGACC
    AGCAGGCACA AACTAAAAGC TGGCAAACTG CTCAGCCCTC TAGTACAACA ACTGAGAAAT
    GTTGCTTCTA AAACTGGTGA CTCCAGCAAA CCTAATGATT TAACCAAATC TGCTGAGGCG
    GTCCCGAGCT TGAATGGATC TCCTGTCAGA GCATCCAACT TGTCTGATGG CAGGCCTTAT
    TCTGGGACCG GGACTCTGCC TTGTTCTGTT TCTGAGCCAG TTCAGACCCA ACAGCCCTGC
    TCTCTTAAAG GATCTCTGTC TTCCGACAAT ATCTACTATG GTGCACAGGG AGAAGGACCC
    GTGCCACGCA GCCAGCCTGC CCAAGGCTGG CCTCCTTCTC CCCAGGCGTC TGCGCAGGTC
    ACCTATAAAT CCAGTAGTAA ACCACGCGCT AGATTCCTCA GGTCAACACT TAAGCGCCTG
    TGTCTGGGTA AAGAACGTGG CAGCATTCAT TCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304512.1
    CDS716..7951
    Translation

    Target ORF information:

    RefSeq Version XM_009306237.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306237.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC AGCGACATGG CAGTATTCCT GGTGACTCCC AAGGAATATT CTCTAATAAC
    TTGGCGGGGA CCAGGCGCAG CAGCACATCA TTCCTCGACA CATTACAGAG AAGGGCCGAC
    CTACAAGATT TTTTAGAACG CCTTCATTAT CAGGCATATA GCTGTCCCAA ACCTGGAGCA
    AGTTCTAGAT ATAAAATAAA TCAGTCGCAG TCTACAAGTG CCTTTGTAGG GTCTTCATCA
    TTCACAAATG ATGAGCAGGA AACAGATATT TCTAAAGACA TTGGAGACTT AGGACATAGA
    TCAGAACAAG TAAAAATTCA TTCTCAGTCA ATGTCATTCG GTCGAAGGAA TTCTGATATC
    CCCCCACATT ACTGCCAGGC TTGTAGGGCA ATGTTGCTGG GTGTAAGGTC AGGGGGTCGA
    GGGTCTCTTG GCCATGCTTC TTCCTCCCAT ATCCACCAAA ATAAGTCCAC TGTATTCCCA
    AATAAACCAC CTTTGTCACC TGATCCGGCT CATCTAGATT CAATTTATAT GACTCAACAG
    TCTCCACATA TCCCTCCAAA GCACGTTTCT GTTCCTAAGA ACATATCAAC TTCAATACCA
    GTTCAAGTCA CCCCACCCTC TCCTGGACAC GAAGGCCATC CTCCTGCTCA GGTTCCTGTC
    ACAGGATTAA ATCATAGCAC TGCCTTCTCC CCTGTCTATA TAACAATACC CTCTCCAGTC
    CACACTTCTT CCAGACATAC ATCTATATCT TCTCCAGTTG ATTTAGTCTC CCCCCTCTGG
    CCATCCTCTC CAGTGTCCCC TGTCTCCTTG ATTGGGCCAC ATTCTCCTAG TTTGGGAGAC
    AGCTCAGATT TGTCTCACCT TGACCGCTGT CTCCATCATA TCATCAATCG TCGTACTAGC
    TCCCCTGTGC TAGCTGACAG GGCACAGCCT GGAGATGGCC ACGGCGATTC TTCTATTAAC
    AGGTTTCTTG GACTTCAGTT CCAAGCCAAA AGCTCTTTGT GTGTACCTCT ATCACATCAG
    GAAACTTTTT CTGTGCAGGA CAGTGAGGTT GGACCAGTTC TTGTGCCTTC TGCACAAACT
    GTTCCATCTA AGACTGTCTC TGCTCCAGCT ACTCCTGTAC CGCATGCCCG TCCACAAAGC
    ATTCCTGCTT CTGCCCTTGT AGCACAGCAA AACCAGACAA CTGTACCACT AGCCCAGGAG
    TTTCATCTCT ATTTCCATCC TGAAGCTTTC CTGCCTCAGA CTTTATCAAC TCTCCAGCCA
    CTAATGCAGG CATCCACAGA TGGTCAGTCT ATTCCACAGC ATCTATTACA GTCAGCATCT
    TCAATGCTCA GTACCCTACC TCCACAACCA CAACTAAATA TTCAGACACC CCTTCTCCAG
    CCTCTACAAA TTTCCACAAA GTTTCCTTCG ACATATCCTC TTTTACCAGA AGGGGGCACA
    TCTACAGGGA CAGAACCTAT ATCTTTCTCC TCAGTCTCTT ACTCTTCTCC TTATCCCACA
    AATGCTCCTC CTGCATCATC CCCCTACTAC TCACCAAGCC CCATCAGCGC TCCCCTTCCC
    ATAATACCCA TGCAGAATGT ACCTTGCATA CTAGGATCAG GCACACCTGT ATCCACCCCT
    CTCAATGTTC CCACCCCTAT ACCTCTCCTG GCTATGGCCC TCTCCCCACC CATGCCTCCA
    CCAGAGCAAC ATCTGCAACA GATGTTCTCC CCAATTCCAA CACCAGAAGG TGCCCTTTAC
    CAACCTCAAC CCCAACCAAC ACAGCCCTCC CTTCCCCTTC CAAATCCAGC ATCTCTCCCT
    CAGCAAGACC CGTCTATTCC TGAACCTTTT AAAGAGGAAT ACTCTCACCA TGAGGAATTG
    CAGATCCTGG CTCCATCTCA CACTGTTATG TCACAGATTC AGTCTCCTCC ACAGGACAGT
    AAGTCAGAAA CAAAGTTTAC CAATGTCCAA GCCTTTTCTG AGACAGTGGA GCATCCTCTG
    CAGTCTACTG GCATTCAGAG AGCTTATGCT GTTCCTGAGA GTACTGGCAC AGCTAGTGCA
    ACTCAGCAAA GGGCAGAAAC TTCTGCAATG CCCAAACTCC CAGCACCAGA GCCCTGCCCA
    TTTCAACACA GCACTGAGGC ACCTGCATCT ATTCCTCTCC ATGCTTTTGT GTGCGACAGC
    CTAAATCAAG ATGCAGGATC TAGTAAAGAA ATGAGTGACA GCTATGAAGG ATCCACCGGT
    GGAGGAAAAG GTGATGGCAA ACCCAGAAAG CATCACCGCA AATCTGCTCG CACACGTTCA
    CGCCAAGAAA AAATTAACAA GCCAAAACTA AGCATGTTAA ATGTTAGTAA TACGGGTGAC
    AAGATGGTGG AATGCCAGTT GGAGACACAC AATCATAAAA TGGTGACTTT CAAGTTCGAC
    CTAGATGGAG ATGCGCCTGA AGAAATAGCC ACTTATATGG TGGAAAATGG TTTCATTCTA
    CCAATAGAAA AGGAGATTTT CATAGATCAA CTAAAAGACA TTGTTGACAA GGCAGAGGAT
    ATTTTAAGTG AGGACATGGA TGGAGAGAAG ACATCAGAAC TTGGAAAAGG ACAGACACCT
    GGAGATAAAG GAAGAGAGGC AATAAATGGA CAGCAACCTG GGATTCCCCA GGCAGTTTAT
    CAGCAAAATG TTCTGCACAC AGGCAAGAGG TGGTTCATCA TCTGCCCTGT AGAAGAAGCT
    CCTGCAGCAA GTCAGGATGG ATGCTCAGAT GGAGGTCCAT CTACACAATC TCCATCTACA
    ACAACAATAA CTGAAGGAAC AGCCACTGCT CAGCCTGTAG AGAGTGCATC AGTACAACCA
    CCAGAACCCA GCACAGGATC TGCTTCCGCT TCTATGGATT CAGAAACCTG TGCCACAGCG
    CCTCCATCTG GAGGAAGTGA TCCCTATGGG GTCTGGAGCC CTCTCTCTTT GACAAATACT
    GATCCTCTTT CTCTGGCTGC TCTTTCCCAA GCTCCTCCTG CCCCGCAAGC AGCTATGATG
    CCAGCACAGC CTGCTTCACA TTCAGAGGAG GGCCAAAATT TGGGCTCTAC CCAGGTCAGT
    GTACAGCAGT CCCAGCCCTG CATAGATCCT CAAAGCTCTC TTTTTGTGGA AGAGACTCAA
    AGTGGCAGAT TAGACTCGGT CTCCGCAATG CACACCGCTC AGCAAATGGC TGAAATTGCA
    TGTGCTGCCT CATTGGTGGA GGACGTGCCC TGCTGTCCCT TGGTCATGCC GTTGTCCCTT
    GAAGTAAGCA GCGGGGCTCA GAAGCCATCA ACCGTAATTC CGCCACCATC ACAAGACAGT
    ACATCTGCTC GCGAACAACT CCAATCCATT ACATCTAATC GAGCGGAGCG TGGCCTGCAA
    CCTGTGGTGC TGCAGCAGCC TGTTTCCACT GTAAGTGGAT CTAAGGCTCC TTCCCTGCCT
    CAGAGCCCTG CTCCTTCACA GCACCACTTT GTCCCTGGTG AGTCAGATGG AGAGGGGCGT
    AGCAGAGGAG GGTTTGTAGA CAGCACTATC AAGACTCTGG ATGAGAAATT GAGGAATTTA
    TTGTACCAAG AGTACGCTCC GATGTACCCA TCGGGAAGTG CAGCTGAAAC TCCAGGATCT
    GGTACAGAGT ATATCCAGTC TCCACCAGGA CCTGAGAGTG CCATGGGAGG GTCAGGAACC
    AGCACACCTA GTCTTATAGG AGAGGGACGA TTCAGGGCAG GTGAACAGCT GCCACAGATC
    CCAGAGCGGG TAGACAGTTT AAGCGCTCTC AGCGACTCTG CAGTTGGAGT TACTGTGTCA
    AGGAGACATG TGATGCCACA CTCTACATCT TGTTCTGGAT CTAGAAATCG ATATAAGATG
    GTGCCAAGCT CCACTGACAT CTTGACAGGT CAAGGTCGAA AGCAGCGCAG CCTGAGCAGC
    ACGGCATCTC CAGCACACCC TGGTGGATTT TTGGGAGAAT GTACCATGTA CGAAGAGCCG
    ACTATGTCAG CTACTACAGT TGGCAGGTTC TCAGTGGTCA GCACAGAAGA CGAGGTCACG
    CGTAGAAAGT ACAGTAGCAG ATATTCAGCT CCACCTGACT TTTATCTGGA CGCTCCACCT
    CCACTGACCA AACGGGGGTC TTTGCCAAGG GCGCAGACCT CGACTTCAGC AGATGCCACA
    GTTTGCACAC GATTTATGTC ATCTGACTCT GGGGCAGAAA GCAGCCCTGC AAAAATGGCA
    CCTACAACCC CATCCCGGCA TGGCAGGTCT GAAAGGAGAG GAAGCGACCT CATGAAAAGG
    GCAGTGGCTT TTCTAAAGCG CTCTGGCCGC AGCAGTAGTG TGCAAAGCTC TGATTCACCA
    AGCCGGAAAG GAGGGGTGTA TGGATCCTAT GTCAGCAGTG ACAATGACTC AGAGATGGAG
    GACTCAGATA TTAAAAAGGA GCTTCAAAAT CTAAGAGAAA AGCATATGAA GGAGATAAGT
    GAATTGGAGG CCCACCACAG AGAAGAGATT GAGCTTCTCT ATGTCAGATT AGGAAAACCA
    CCACCTCCAG GTCTATACAT CCCACCCACA GTGCCCCCTG CTGGACGCAA GCGCAGGACC
    AGCAGGCACA AACTAAAAGC TGGCAAACTG CTCAGCCCTC TAGTACAACA ACTGAGAAAT
    GTTGCTTCTA AAACTGGTGA CTCCAGCAAA CCTAATGATT TAACCAAATC TGCTGAGGCG
    GTCCCGAGCT TGAATGGATC TCCTGTCAGA GCATCCAACT TGTCTGATGG CAGGCCTTAT
    TCTGGGACCG GGACTCTGCC TTGTTCTGTT TCTGAGCCAG TTCAGACCCA ACAGCCCTGC
    TCTCTTAAAG GATCTCTGTC TTCCGACAAT ATCTACTATG GTGCACAGGG AGAAGGACCC
    GTGCCACGCA GCCAGCCTGC CCAAGGCTGG CCTCCTTCTC CCCAGGCGTC TGCGCAGGTC
    ACCTATAAAT CCAGTAGTAA ACCACGCGCT AGATTCCTCA GGTCAACACT TAAGCGCCTG
    TGTCTGGGTA AAGAACGTGG CAGCATTCAT TCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53091
    Clone ID Related Accession (Same CDS sequence) XM_009306238.2 , XM_021479884.1 , XM_009306238.3
    Accession Version XM_009306238.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306238.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGTTTCAT CTCTATTTCC ATCCTGAAGC TTTCCTGCCT
    CAGACTTTAT CAACTCTCCA GCCACTAATG CAGGCATCCA CAGATGGTCA GTCTATTCCA
    CAGCATCTAT TACAGTCAGC ATCTTCAATG CTCAGTACCC TACCTCCACA ACCACAACTA
    AATATTCAGA CACCCCTTCT CCAGCCTCTA CAAATTTCCA CAAAGTTTCC TTCGACATAT
    CCTCTTTTAC CAGAAGGGGG CACATCTACA GGGACAGAAC CTATATCTTT CTCCTCAGTC
    TCTTACTCTT CTCCTTATCC CACAAATGCT CCTCCTGCAT CATCCCCCTA CTACTCACCA
    AGCCCCATCA GCGCTCCCCT TCCCATAATA CCCATGCAGA ATGTACCTTG CATACTAGGA
    TCAGGCACAC CTGTATCCAC CCCTCTCAAT GTTCCCACCC CTATACCTCT CCTGGCTATG
    GCCCTCTCCC CACCCATGCC TCCACCAGAG CAACATCTGC AACAGATGTT CTCCCCAATT
    CCAACACCAG AAGGTGCCCT TTACCAACCT CAACCCCAAC CAACACAGCC CTCCCTTCCC
    CTTCCAAATC CAGCATCTCT CCCTCAGCAA GACCCGTCTA TTCCTGAACC TTTTAAAGAG
    GAATACTCTC ACCATGAGGA ATTGCAGATC CTGGCTCCAT CTCACACTGT TATGTCACAG
    ATTCAGTCTC CTCCACAGGA CAGTAAGTCA GAAACAAAGT TTACCAATGT CCAAGCCTTT
    TCTGAGACAG TGGAGCATCC TCTGCAGTCT ACTGGCATTC AGAGAGCTTA TGCTGTTCCT
    GAGAGTACTG GCACAGCTAG TGCAACTCAG CAAAGGGCAG AAACTTCTGC AATGCCCAAA
    CTCCCAGCAC CAGAGCCCTG CCCATTTCAA CACAGCACTG AGGCACCTGC ATCTATTCCT
    CTCCATGCTT TTGTGTGCGA CAGCCTAAAT CAAGATGCAG GATCTAGTAA AGAAATGAGT
    GACAGCTATG AAGGATCCAC CGGTGGAGGA AAAGGTGATG GCAAACCCAG AAAGCATCAC
    CGCAAATCTG CTCGCACACG TTCACGCCAA GAAAAAATTA ACAAGCCAAA ACTAAGCATG
    TTAAATGTTA GTAATACGGG TGACAAGATG GTGGAATGCC AGTTGGAGAC ACACAATCAT
    AAAATGGTGA CTTTCAAGTT CGACCTAGAT GGAGATGCGC CTGAAGAAAT AGCCACTTAT
    ATGGTGGAAA ATGGTTTCAT TCTACCAATA GAAAAGGAGA TTTTCATAGA TCAACTAAAA
    GACATTGTTG ACAAGGCAGA GGATATTTTA AGTGAGGACA TGGATGGAGA GAAGACATCA
    GAACTTGGAA AAGGACAGAC ACCTGGAGAT AAAGGAAGAG AGGCAATAAA TGGACAGCAA
    CCTGGGATTC CCCAGGCAGT TTATCAGCAA AATGTTCTGC ACACAGGCAA GAGGTGGTTC
    ATCATCTGCC CTGTAGAAGA AGCTCCTGCA GCAAGTCAGG ATGGATGCTC AGATGGAGGT
    CCATCTACAC AATCTCCATC TACAACAACA ATAACTGAAG GAACAGCCAC TGCTCAGCCT
    GTAGAGAGTG CATCAGTACA ACCACCAGAA CCCAGCACAG GATCTGCTTC CGCTTCTATG
    GATTCAGAAA CCTGTGCCAC AGCGCCTCCA TCTGGAGGAA GTGATCCCTA TGGGGTCTGG
    AGCCCTCTCT CTTTGACAAA TACTGATCCT CTTTCTCTGG CTGCTCTTTC CCAAGCTCCT
    CCTGCCCCGC AAGCAGCTAT GATGCCAGCA CAGCCTGCTT CACATTCAGA GGAGGGCCAA
    AATTTGGGCT CTACCCAGGT CAGTGTACAG CAGTCCCAGC CCTGCATAGA TCCTCAAAGC
    TCTCTTTTTG TGGAAGAGAC TCAAAGTGGC AGATTAGACT CGGTCTCCGC AATGCACACC
    GCTCAGCAAA TGGCTGAAAT TGCATGTGCT GCCTCATTGG TGGAGGACGT GCCCTGCTGT
    CCCTTGGTCA TGCCGTTGTC CCTTGAAGTA AGCAGCGGGG CTCAGAAGCC ATCAACCGTA
    ATTCCGCCAC CATCACAAGA CAGTACATCT GCTCGCGAAC AACTCCAATC CATTACATCT
    AATCGAGCGG AGCGTGGCCT GCAACCTGTG GTGCTGCAGC AGCCTGTTTC CACTGTAAGT
    GGATCTAAGG CTCCTTCCCT GCCTCAGAGC CCTGCTCCTT CACAGCACCA CTTTGTCCCT
    GGTGAGTCAG ATGGAGAGGG GCGTAGCAGA GGAGGGTTTG TAGACAGCAC TATCAAGACT
    CTGGATGAGA AATTGAGGAA TTTATTGTAC CAAGAGTACG CTCCGATGTA CCCATCGGGA
    AGTGCAGCTG AAACTCCAGG ATCTGGTACA GAGTATATCC AGTCTCCACC AGGACCTGAG
    AGTGCCATGG GAGGGTCAGG AACCAGCACA CCTAGTCTTA TAGGAGAGGG ACGATTCAGG
    GCAGGTGAAC AGCTGCCACA GATCCCAGAG CGGGTAGACA GTTTAAGCGC TCTCAGCGAC
    TCTGCAGTTG GAGTTACTGT GTCAAGGAGA CATGTGATGC CACACTCTAC ATCTTGTTCT
    GGATCTAGAA ATCGATATAA GATGGTGCCA AGCTCCACTG ACATCTTGAC AGGTCAAGGT
    CGAAAGCAGC GCAGCCTGAG CAGCACGGCA TCTCCAGCAC ACCCTGGTGG ATTTTTGGGA
    GAATGTACCA TGTACGAAGA GCCGACTATG TCAGCTACTA CAGTTGGCAG GTTCTCAGTG
    GTCAGCACAG AAGACGAGGT CACGCGTAGA AAGTACAGTA GCAGATATTC AGCTCCACCT
    GACTTTTATC TGGACGCTCC ACCTCCACTG ACCAAACGGG GGTCTTTGCC AAGGGCGCAG
    ACCTCGACTT CAGCAGATGC CACAGTTTGC ACACGATTTA TGTCATCTGA CTCTGGGGCA
    GAAAGCAGCC CTGCAAAAAT GGCACCTACA ACCCCATCCC GGCATGGCAG GTCTGAAAGG
    AGAGGAAGCG ACCTCATGAA AAGGGCAGTG GCTTTTCTAA AGCGCTCTGG CCGCAGCAGT
    AGTGTGCAAA GCTCTGATTC ACCAAGCCGG AAAGGAGGGG TGTATGGATC CTATGTCAGC
    AGTGACAATG ACTCAGAGAT GGAGGACTCA GATATTAAAA AGGAGCTTCA AAATCTAAGA
    GAAAAGCATA TGAAGGAGAT AAGTGAATTG GAGGCCCACC ACAGAGAAGA GATTGAGCTT
    CTCTATGTCA GATTAGGAAA ACCACCACCT CCAGGTCTAT ACATCCCACC CACAGTGCCC
    CCTGCTGGAC GCAAGCGCAG GACCAGCAGG CACAAACTAA AAGCTGGCAA ACTGCTCAGC
    CCTCTAGTAC AACAACTGAG AAATGTTGCT TCTAAAACTG GTGACTCCAG CAAACCTAAT
    GATTTAACCA AATCTGCTGA GGCGGTCCCG AGCTTGAATG GATCTCCTGT CAGAGCATCC
    AACTTGTCTG ATGGCAGGCC TTATTCTGGG ACCGGGACTC TGCCTTGTTC TGTTTCTGAG
    CCAGTTCAGA CCCAACAGCC CTGCTCTCTT AAAGGATCTC TGTCTTCCGA CAATATCTAC
    TATGGTGCAC AGGGAGAAGG ACCCGTGCCA CGCAGCCAGC CTGCCCAAGG CTGGCCTCCT
    TCTCCCCAGG CGTCTGCGCA GGTCACCTAT AAATCCAGTA GTAAACCACG CGCTAGATTC
    CTCAGTGGGC CAGTTTCTCT GTCCATCTGG TCAACACTTA AGCGCCTGTG TCTGGGTAAA
    GAACGTGGCA GCAGATCTGG AGCATCATCA GGTGCCTCAA ATCAATCCCC TATGCCTCCT
    GGATCCACTC CTCCTCCTCA CCAGCCTGTT GGCCTTGCCC AAGCACAGAC AAACAACAGC
    AACAACAAAA CAGATGCTTT CACTCAGCAG CTTCACCGAC TGGTGGATAA CTGGGCAGAA
    GGTGTGAGGG CTCCTTCACG CTCACGCTCC CTCAGTCTAA GACCACAGCA ACTGACCAGA
    ACCAGGATCT GGAGTGCAAC AGAGGTTCCA GGGTCAATGG AAAGATTGAA TGCTTCCCGG
    CTGCCTCTTT CATGGCCACA GATTGATTTC CATGCCTCTG TGATGGTGAC TGACACTTCA
    CAAGTGCTCC AGCACAGTTT TATGGTGTCC AGTAACCCTT ATGAAAAGAT GCTTAACTCC
    ACGCACTTTG ACAAGGACCT TTGGCCAGCA ATGACTGCCA ATCTTAACCA AGAAGTCTTT
    GCTTTCTCTG CAGCGGACTC TCCGTCCTGG ACAGCACCTT CTTCCCCCAG CGAGGGTCCT
    AGTTCTAGAA ATAGGACCAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304513.1
    CDS705..7448
    Translation

    Target ORF information:

    RefSeq Version XM_009306238.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306238.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGTTTCAT CTCTATTTCC ATCCTGAAGC TTTCCTGCCT
    CAGACTTTAT CAACTCTCCA GCCACTAATG CAGGCATCCA CAGATGGTCA GTCTATTCCA
    CAGCATCTAT TACAGTCAGC ATCTTCAATG CTCAGTACCC TACCTCCACA ACCACAACTA
    AATATTCAGA CACCCCTTCT CCAGCCTCTA CAAATTTCCA CAAAGTTTCC TTCGACATAT
    CCTCTTTTAC CAGAAGGGGG CACATCTACA GGGACAGAAC CTATATCTTT CTCCTCAGTC
    TCTTACTCTT CTCCTTATCC CACAAATGCT CCTCCTGCAT CATCCCCCTA CTACTCACCA
    AGCCCCATCA GCGCTCCCCT TCCCATAATA CCCATGCAGA ATGTACCTTG CATACTAGGA
    TCAGGCACAC CTGTATCCAC CCCTCTCAAT GTTCCCACCC CTATACCTCT CCTGGCTATG
    GCCCTCTCCC CACCCATGCC TCCACCAGAG CAACATCTGC AACAGATGTT CTCCCCAATT
    CCAACACCAG AAGGTGCCCT TTACCAACCT CAACCCCAAC CAACACAGCC CTCCCTTCCC
    CTTCCAAATC CAGCATCTCT CCCTCAGCAA GACCCGTCTA TTCCTGAACC TTTTAAAGAG
    GAATACTCTC ACCATGAGGA ATTGCAGATC CTGGCTCCAT CTCACACTGT TATGTCACAG
    ATTCAGTCTC CTCCACAGGA CAGTAAGTCA GAAACAAAGT TTACCAATGT CCAAGCCTTT
    TCTGAGACAG TGGAGCATCC TCTGCAGTCT ACTGGCATTC AGAGAGCTTA TGCTGTTCCT
    GAGAGTACTG GCACAGCTAG TGCAACTCAG CAAAGGGCAG AAACTTCTGC AATGCCCAAA
    CTCCCAGCAC CAGAGCCCTG CCCATTTCAA CACAGCACTG AGGCACCTGC ATCTATTCCT
    CTCCATGCTT TTGTGTGCGA CAGCCTAAAT CAAGATGCAG GATCTAGTAA AGAAATGAGT
    GACAGCTATG AAGGATCCAC CGGTGGAGGA AAAGGTGATG GCAAACCCAG AAAGCATCAC
    CGCAAATCTG CTCGCACACG TTCACGCCAA GAAAAAATTA ACAAGCCAAA ACTAAGCATG
    TTAAATGTTA GTAATACGGG TGACAAGATG GTGGAATGCC AGTTGGAGAC ACACAATCAT
    AAAATGGTGA CTTTCAAGTT CGACCTAGAT GGAGATGCGC CTGAAGAAAT AGCCACTTAT
    ATGGTGGAAA ATGGTTTCAT TCTACCAATA GAAAAGGAGA TTTTCATAGA TCAACTAAAA
    GACATTGTTG ACAAGGCAGA GGATATTTTA AGTGAGGACA TGGATGGAGA GAAGACATCA
    GAACTTGGAA AAGGACAGAC ACCTGGAGAT AAAGGAAGAG AGGCAATAAA TGGACAGCAA
    CCTGGGATTC CCCAGGCAGT TTATCAGCAA AATGTTCTGC ACACAGGCAA GAGGTGGTTC
    ATCATCTGCC CTGTAGAAGA AGCTCCTGCA GCAAGTCAGG ATGGATGCTC AGATGGAGGT
    CCATCTACAC AATCTCCATC TACAACAACA ATAACTGAAG GAACAGCCAC TGCTCAGCCT
    GTAGAGAGTG CATCAGTACA ACCACCAGAA CCCAGCACAG GATCTGCTTC CGCTTCTATG
    GATTCAGAAA CCTGTGCCAC AGCGCCTCCA TCTGGAGGAA GTGATCCCTA TGGGGTCTGG
    AGCCCTCTCT CTTTGACAAA TACTGATCCT CTTTCTCTGG CTGCTCTTTC CCAAGCTCCT
    CCTGCCCCGC AAGCAGCTAT GATGCCAGCA CAGCCTGCTT CACATTCAGA GGAGGGCCAA
    AATTTGGGCT CTACCCAGGT CAGTGTACAG CAGTCCCAGC CCTGCATAGA TCCTCAAAGC
    TCTCTTTTTG TGGAAGAGAC TCAAAGTGGC AGATTAGACT CGGTCTCCGC AATGCACACC
    GCTCAGCAAA TGGCTGAAAT TGCATGTGCT GCCTCATTGG TGGAGGACGT GCCCTGCTGT
    CCCTTGGTCA TGCCGTTGTC CCTTGAAGTA AGCAGCGGGG CTCAGAAGCC ATCAACCGTA
    ATTCCGCCAC CATCACAAGA CAGTACATCT GCTCGCGAAC AACTCCAATC CATTACATCT
    AATCGAGCGG AGCGTGGCCT GCAACCTGTG GTGCTGCAGC AGCCTGTTTC CACTGTAAGT
    GGATCTAAGG CTCCTTCCCT GCCTCAGAGC CCTGCTCCTT CACAGCACCA CTTTGTCCCT
    GGTGAGTCAG ATGGAGAGGG GCGTAGCAGA GGAGGGTTTG TAGACAGCAC TATCAAGACT
    CTGGATGAGA AATTGAGGAA TTTATTGTAC CAAGAGTACG CTCCGATGTA CCCATCGGGA
    AGTGCAGCTG AAACTCCAGG ATCTGGTACA GAGTATATCC AGTCTCCACC AGGACCTGAG
    AGTGCCATGG GAGGGTCAGG AACCAGCACA CCTAGTCTTA TAGGAGAGGG ACGATTCAGG
    GCAGGTGAAC AGCTGCCACA GATCCCAGAG CGGGTAGACA GTTTAAGCGC TCTCAGCGAC
    TCTGCAGTTG GAGTTACTGT GTCAAGGAGA CATGTGATGC CACACTCTAC ATCTTGTTCT
    GGATCTAGAA ATCGATATAA GATGGTGCCA AGCTCCACTG ACATCTTGAC AGGTCAAGGT
    CGAAAGCAGC GCAGCCTGAG CAGCACGGCA TCTCCAGCAC ACCCTGGTGG ATTTTTGGGA
    GAATGTACCA TGTACGAAGA GCCGACTATG TCAGCTACTA CAGTTGGCAG GTTCTCAGTG
    GTCAGCACAG AAGACGAGGT CACGCGTAGA AAGTACAGTA GCAGATATTC AGCTCCACCT
    GACTTTTATC TGGACGCTCC ACCTCCACTG ACCAAACGGG GGTCTTTGCC AAGGGCGCAG
    ACCTCGACTT CAGCAGATGC CACAGTTTGC ACACGATTTA TGTCATCTGA CTCTGGGGCA
    GAAAGCAGCC CTGCAAAAAT GGCACCTACA ACCCCATCCC GGCATGGCAG GTCTGAAAGG
    AGAGGAAGCG ACCTCATGAA AAGGGCAGTG GCTTTTCTAA AGCGCTCTGG CCGCAGCAGT
    AGTGTGCAAA GCTCTGATTC ACCAAGCCGG AAAGGAGGGG TGTATGGATC CTATGTCAGC
    AGTGACAATG ACTCAGAGAT GGAGGACTCA GATATTAAAA AGGAGCTTCA AAATCTAAGA
    GAAAAGCATA TGAAGGAGAT AAGTGAATTG GAGGCCCACC ACAGAGAAGA GATTGAGCTT
    CTCTATGTCA GATTAGGAAA ACCACCACCT CCAGGTCTAT ACATCCCACC CACAGTGCCC
    CCTGCTGGAC GCAAGCGCAG GACCAGCAGG CACAAACTAA AAGCTGGCAA ACTGCTCAGC
    CCTCTAGTAC AACAACTGAG AAATGTTGCT TCTAAAACTG GTGACTCCAG CAAACCTAAT
    GATTTAACCA AATCTGCTGA GGCGGTCCCG AGCTTGAATG GATCTCCTGT CAGAGCATCC
    AACTTGTCTG ATGGCAGGCC TTATTCTGGG ACCGGGACTC TGCCTTGTTC TGTTTCTGAG
    CCAGTTCAGA CCCAACAGCC CTGCTCTCTT AAAGGATCTC TGTCTTCCGA CAATATCTAC
    TATGGTGCAC AGGGAGAAGG ACCCGTGCCA CGCAGCCAGC CTGCCCAAGG CTGGCCTCCT
    TCTCCCCAGG CGTCTGCGCA GGTCACCTAT AAATCCAGTA GTAAACCACG CGCTAGATTC
    CTCAGTGGGC CAGTTTCTCT GTCCATCTGG TCAACACTTA AGCGCCTGTG TCTGGGTAAA
    GAACGTGGCA GCAGATCTGG AGCATCATCA GGTGCCTCAA ATCAATCCCC TATGCCTCCT
    GGATCCACTC CTCCTCCTCA CCAGCCTGTT GGCCTTGCCC AAGCACAGAC AAACAACAGC
    AACAACAAAA CAGATGCTTT CACTCAGCAG CTTCACCGAC TGGTGGATAA CTGGGCAGAA
    GGTGTGAGGG CTCCTTCACG CTCACGCTCC CTCAGTCTAA GACCACAGCA ACTGACCAGA
    ACCAGGATCT GGAGTGCAAC AGAGGTTCCA GGGTCAATGG AAAGATTGAA TGCTTCCCGG
    CTGCCTCTTT CATGGCCACA GATTGATTTC CATGCCTCTG TGATGGTGAC TGACACTTCA
    CAAGTGCTCC AGCACAGTTT TATGGTGTCC AGTAACCCTT ATGAAAAGAT GCTTAACTCC
    ACGCACTTTG ACAAGGACCT TTGGCCAGCA ATGACTGCCA ATCTTAACCA AGAAGTCTTT
    GCTTTCTCTG CAGCGGACTC TCCGTCCTGG ACAGCACCTT CTTCCCCCAG CGAGGGTCCT
    AGTTCTAGAA ATAGGACCAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53091
    Clone ID Related Accession (Same CDS sequence) XM_009306238.2 , XM_021479884.1 , XM_009306238.3
    Accession Version XM_021479884.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGTTTCAT CTCTATTTCC ATCCTGAAGC TTTCCTGCCT
    CAGACTTTAT CAACTCTCCA GCCACTAATG CAGGCATCCA CAGATGGTCA GTCTATTCCA
    CAGCATCTAT TACAGTCAGC ATCTTCAATG CTCAGTACCC TACCTCCACA ACCACAACTA
    AATATTCAGA CACCCCTTCT CCAGCCTCTA CAAATTTCCA CAAAGTTTCC TTCGACATAT
    CCTCTTTTAC CAGAAGGGGG CACATCTACA GGGACAGAAC CTATATCTTT CTCCTCAGTC
    TCTTACTCTT CTCCTTATCC CACAAATGCT CCTCCTGCAT CATCCCCCTA CTACTCACCA
    AGCCCCATCA GCGCTCCCCT TCCCATAATA CCCATGCAGA ATGTACCTTG CATACTAGGA
    TCAGGCACAC CTGTATCCAC CCCTCTCAAT GTTCCCACCC CTATACCTCT CCTGGCTATG
    GCCCTCTCCC CACCCATGCC TCCACCAGAG CAACATCTGC AACAGATGTT CTCCCCAATT
    CCAACACCAG AAGGTGCCCT TTACCAACCT CAACCCCAAC CAACACAGCC CTCCCTTCCC
    CTTCCAAATC CAGCATCTCT CCCTCAGCAA GACCCGTCTA TTCCTGAACC TTTTAAAGAG
    GAATACTCTC ACCATGAGGA ATTGCAGATC CTGGCTCCAT CTCACACTGT TATGTCACAG
    ATTCAGTCTC CTCCACAGGA CAGTAAGTCA GAAACAAAGT TTACCAATGT CCAAGCCTTT
    TCTGAGACAG TGGAGCATCC TCTGCAGTCT ACTGGCATTC AGAGAGCTTA TGCTGTTCCT
    GAGAGTACTG GCACAGCTAG TGCAACTCAG CAAAGGGCAG AAACTTCTGC AATGCCCAAA
    CTCCCAGCAC CAGAGCCCTG CCCATTTCAA CACAGCACTG AGGCACCTGC ATCTATTCCT
    CTCCATGCTT TTGTGTGCGA CAGCCTAAAT CAAGATGCAG GATCTAGTAA AGAAATGAGT
    GACAGCTATG AAGGATCCAC CGGTGGAGGA AAAGGTGATG GCAAACCCAG AAAGCATCAC
    CGCAAATCTG CTCGCACACG TTCACGCCAA GAAAAAATTA ACAAGCCAAA ACTAAGCATG
    TTAAATGTTA GTAATACGGG TGACAAGATG GTGGAATGCC AGTTGGAGAC ACACAATCAT
    AAAATGGTGA CTTTCAAGTT CGACCTAGAT GGAGATGCGC CTGAAGAAAT AGCCACTTAT
    ATGGTGGAAA ATGGTTTCAT TCTACCAATA GAAAAGGAGA TTTTCATAGA TCAACTAAAA
    GACATTGTTG ACAAGGCAGA GGATATTTTA AGTGAGGACA TGGATGGAGA GAAGACATCA
    GAACTTGGAA AAGGACAGAC ACCTGGAGAT AAAGGAAGAG AGGCAATAAA TGGACAGCAA
    CCTGGGATTC CCCAGGCAGT TTATCAGCAA AATGTTCTGC ACACAGGCAA GAGGTGGTTC
    ATCATCTGCC CTGTAGAAGA AGCTCCTGCA GCAAGTCAGG ATGGATGCTC AGATGGAGGT
    CCATCTACAC AATCTCCATC TACAACAACA ATAACTGAAG GAACAGCCAC TGCTCAGCCT
    GTAGAGAGTG CATCAGTACA ACCACCAGAA CCCAGCACAG GATCTGCTTC CGCTTCTATG
    GATTCAGAAA CCTGTGCCAC AGCGCCTCCA TCTGGAGGAA GTGATCCCTA TGGGGTCTGG
    AGCCCTCTCT CTTTGACAAA TACTGATCCT CTTTCTCTGG CTGCTCTTTC CCAAGCTCCT
    CCTGCCCCGC AAGCAGCTAT GATGCCAGCA CAGCCTGCTT CACATTCAGA GGAGGGCCAA
    AATTTGGGCT CTACCCAGGT CAGTGTACAG CAGTCCCAGC CCTGCATAGA TCCTCAAAGC
    TCTCTTTTTG TGGAAGAGAC TCAAAGTGGC AGATTAGACT CGGTCTCCGC AATGCACACC
    GCTCAGCAAA TGGCTGAAAT TGCATGTGCT GCCTCATTGG TGGAGGACGT GCCCTGCTGT
    CCCTTGGTCA TGCCGTTGTC CCTTGAAGTA AGCAGCGGGG CTCAGAAGCC ATCAACCGTA
    ATTCCGCCAC CATCACAAGA CAGTACATCT GCTCGCGAAC AACTCCAATC CATTACATCT
    AATCGAGCGG AGCGTGGCCT GCAACCTGTG GTGCTGCAGC AGCCTGTTTC CACTGTAAGT
    GGATCTAAGG CTCCTTCCCT GCCTCAGAGC CCTGCTCCTT CACAGCACCA CTTTGTCCCT
    GGTGAGTCAG ATGGAGAGGG GCGTAGCAGA GGAGGGTTTG TAGACAGCAC TATCAAGACT
    CTGGATGAGA AATTGAGGAA TTTATTGTAC CAAGAGTACG CTCCGATGTA CCCATCGGGA
    AGTGCAGCTG AAACTCCAGG ATCTGGTACA GAGTATATCC AGTCTCCACC AGGACCTGAG
    AGTGCCATGG GAGGGTCAGG AACCAGCACA CCTAGTCTTA TAGGAGAGGG ACGATTCAGG
    GCAGGTGAAC AGCTGCCACA GATCCCAGAG CGGGTAGACA GTTTAAGCGC TCTCAGCGAC
    TCTGCAGTTG GAGTTACTGT GTCAAGGAGA CATGTGATGC CACACTCTAC ATCTTGTTCT
    GGATCTAGAA ATCGATATAA GATGGTGCCA AGCTCCACTG ACATCTTGAC AGGTCAAGGT
    CGAAAGCAGC GCAGCCTGAG CAGCACGGCA TCTCCAGCAC ACCCTGGTGG ATTTTTGGGA
    GAATGTACCA TGTACGAAGA GCCGACTATG TCAGCTACTA CAGTTGGCAG GTTCTCAGTG
    GTCAGCACAG AAGACGAGGT CACGCGTAGA AAGTACAGTA GCAGATATTC AGCTCCACCT
    GACTTTTATC TGGACGCTCC ACCTCCACTG ACCAAACGGG GGTCTTTGCC AAGGGCGCAG
    ACCTCGACTT CAGCAGATGC CACAGTTTGC ACACGATTTA TGTCATCTGA CTCTGGGGCA
    GAAAGCAGCC CTGCAAAAAT GGCACCTACA ACCCCATCCC GGCATGGCAG GTCTGAAAGG
    AGAGGAAGCG ACCTCATGAA AAGGGCAGTG GCTTTTCTAA AGCGCTCTGG CCGCAGCAGT
    AGTGTGCAAA GCTCTGATTC ACCAAGCCGG AAAGGAGGGG TGTATGGATC CTATGTCAGC
    AGTGACAATG ACTCAGAGAT GGAGGACTCA GATATTAAAA AGGAGCTTCA AAATCTAAGA
    GAAAAGCATA TGAAGGAGAT AAGTGAATTG GAGGCCCACC ACAGAGAAGA GATTGAGCTT
    CTCTATGTCA GATTAGGAAA ACCACCACCT CCAGGTCTAT ACATCCCACC CACAGTGCCC
    CCTGCTGGAC GCAAGCGCAG GACCAGCAGG CACAAACTAA AAGCTGGCAA ACTGCTCAGC
    CCTCTAGTAC AACAACTGAG AAATGTTGCT TCTAAAACTG GTGACTCCAG CAAACCTAAT
    GATTTAACCA AATCTGCTGA GGCGGTCCCG AGCTTGAATG GATCTCCTGT CAGAGCATCC
    AACTTGTCTG ATGGCAGGCC TTATTCTGGG ACCGGGACTC TGCCTTGTTC TGTTTCTGAG
    CCAGTTCAGA CCCAACAGCC CTGCTCTCTT AAAGGATCTC TGTCTTCCGA CAATATCTAC
    TATGGTGCAC AGGGAGAAGG ACCCGTGCCA CGCAGCCAGC CTGCCCAAGG CTGGCCTCCT
    TCTCCCCAGG CGTCTGCGCA GGTCACCTAT AAATCCAGTA GTAAACCACG CGCTAGATTC
    CTCAGTGGGC CAGTTTCTCT GTCCATCTGG TCAACACTTA AGCGCCTGTG TCTGGGTAAA
    GAACGTGGCA GCAGATCTGG AGCATCATCA GGTGCCTCAA ATCAATCCCC TATGCCTCCT
    GGATCCACTC CTCCTCCTCA CCAGCCTGTT GGCCTTGCCC AAGCACAGAC AAACAACAGC
    AACAACAAAA CAGATGCTTT CACTCAGCAG CTTCACCGAC TGGTGGATAA CTGGGCAGAA
    GGTGTGAGGG CTCCTTCACG CTCACGCTCC CTCAGTCTAA GACCACAGCA ACTGACCAGA
    ACCAGGATCT GGAGTGCAAC AGAGGTTCCA GGGTCAATGG AAAGATTGAA TGCTTCCCGG
    CTGCCTCTTT CATGGCCACA GATTGATTTC CATGCCTCTG TGATGGTGAC TGACACTTCA
    CAAGTGCTCC AGCACAGTTT TATGGTGTCC AGTAACCCTT ATGAAAAGAT GCTTAACTCC
    ACGCACTTTG ACAAGGACCT TTGGCCAGCA ATGACTGCCA ATCTTAACCA AGAAGTCTTT
    GCTTTCTCTG CAGCGGACTC TCCGTCCTGG ACAGCACCTT CTTCCCCCAG CGAGGGTCCT
    AGTTCTAGAA ATAGGACCAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335559.1
    CDS828..7571
    Translation

    Target ORF information:

    RefSeq Version XM_021479884.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGTTTCAT CTCTATTTCC ATCCTGAAGC TTTCCTGCCT
    CAGACTTTAT CAACTCTCCA GCCACTAATG CAGGCATCCA CAGATGGTCA GTCTATTCCA
    CAGCATCTAT TACAGTCAGC ATCTTCAATG CTCAGTACCC TACCTCCACA ACCACAACTA
    AATATTCAGA CACCCCTTCT CCAGCCTCTA CAAATTTCCA CAAAGTTTCC TTCGACATAT
    CCTCTTTTAC CAGAAGGGGG CACATCTACA GGGACAGAAC CTATATCTTT CTCCTCAGTC
    TCTTACTCTT CTCCTTATCC CACAAATGCT CCTCCTGCAT CATCCCCCTA CTACTCACCA
    AGCCCCATCA GCGCTCCCCT TCCCATAATA CCCATGCAGA ATGTACCTTG CATACTAGGA
    TCAGGCACAC CTGTATCCAC CCCTCTCAAT GTTCCCACCC CTATACCTCT CCTGGCTATG
    GCCCTCTCCC CACCCATGCC TCCACCAGAG CAACATCTGC AACAGATGTT CTCCCCAATT
    CCAACACCAG AAGGTGCCCT TTACCAACCT CAACCCCAAC CAACACAGCC CTCCCTTCCC
    CTTCCAAATC CAGCATCTCT CCCTCAGCAA GACCCGTCTA TTCCTGAACC TTTTAAAGAG
    GAATACTCTC ACCATGAGGA ATTGCAGATC CTGGCTCCAT CTCACACTGT TATGTCACAG
    ATTCAGTCTC CTCCACAGGA CAGTAAGTCA GAAACAAAGT TTACCAATGT CCAAGCCTTT
    TCTGAGACAG TGGAGCATCC TCTGCAGTCT ACTGGCATTC AGAGAGCTTA TGCTGTTCCT
    GAGAGTACTG GCACAGCTAG TGCAACTCAG CAAAGGGCAG AAACTTCTGC AATGCCCAAA
    CTCCCAGCAC CAGAGCCCTG CCCATTTCAA CACAGCACTG AGGCACCTGC ATCTATTCCT
    CTCCATGCTT TTGTGTGCGA CAGCCTAAAT CAAGATGCAG GATCTAGTAA AGAAATGAGT
    GACAGCTATG AAGGATCCAC CGGTGGAGGA AAAGGTGATG GCAAACCCAG AAAGCATCAC
    CGCAAATCTG CTCGCACACG TTCACGCCAA GAAAAAATTA ACAAGCCAAA ACTAAGCATG
    TTAAATGTTA GTAATACGGG TGACAAGATG GTGGAATGCC AGTTGGAGAC ACACAATCAT
    AAAATGGTGA CTTTCAAGTT CGACCTAGAT GGAGATGCGC CTGAAGAAAT AGCCACTTAT
    ATGGTGGAAA ATGGTTTCAT TCTACCAATA GAAAAGGAGA TTTTCATAGA TCAACTAAAA
    GACATTGTTG ACAAGGCAGA GGATATTTTA AGTGAGGACA TGGATGGAGA GAAGACATCA
    GAACTTGGAA AAGGACAGAC ACCTGGAGAT AAAGGAAGAG AGGCAATAAA TGGACAGCAA
    CCTGGGATTC CCCAGGCAGT TTATCAGCAA AATGTTCTGC ACACAGGCAA GAGGTGGTTC
    ATCATCTGCC CTGTAGAAGA AGCTCCTGCA GCAAGTCAGG ATGGATGCTC AGATGGAGGT
    CCATCTACAC AATCTCCATC TACAACAACA ATAACTGAAG GAACAGCCAC TGCTCAGCCT
    GTAGAGAGTG CATCAGTACA ACCACCAGAA CCCAGCACAG GATCTGCTTC CGCTTCTATG
    GATTCAGAAA CCTGTGCCAC AGCGCCTCCA TCTGGAGGAA GTGATCCCTA TGGGGTCTGG
    AGCCCTCTCT CTTTGACAAA TACTGATCCT CTTTCTCTGG CTGCTCTTTC CCAAGCTCCT
    CCTGCCCCGC AAGCAGCTAT GATGCCAGCA CAGCCTGCTT CACATTCAGA GGAGGGCCAA
    AATTTGGGCT CTACCCAGGT CAGTGTACAG CAGTCCCAGC CCTGCATAGA TCCTCAAAGC
    TCTCTTTTTG TGGAAGAGAC TCAAAGTGGC AGATTAGACT CGGTCTCCGC AATGCACACC
    GCTCAGCAAA TGGCTGAAAT TGCATGTGCT GCCTCATTGG TGGAGGACGT GCCCTGCTGT
    CCCTTGGTCA TGCCGTTGTC CCTTGAAGTA AGCAGCGGGG CTCAGAAGCC ATCAACCGTA
    ATTCCGCCAC CATCACAAGA CAGTACATCT GCTCGCGAAC AACTCCAATC CATTACATCT
    AATCGAGCGG AGCGTGGCCT GCAACCTGTG GTGCTGCAGC AGCCTGTTTC CACTGTAAGT
    GGATCTAAGG CTCCTTCCCT GCCTCAGAGC CCTGCTCCTT CACAGCACCA CTTTGTCCCT
    GGTGAGTCAG ATGGAGAGGG GCGTAGCAGA GGAGGGTTTG TAGACAGCAC TATCAAGACT
    CTGGATGAGA AATTGAGGAA TTTATTGTAC CAAGAGTACG CTCCGATGTA CCCATCGGGA
    AGTGCAGCTG AAACTCCAGG ATCTGGTACA GAGTATATCC AGTCTCCACC AGGACCTGAG
    AGTGCCATGG GAGGGTCAGG AACCAGCACA CCTAGTCTTA TAGGAGAGGG ACGATTCAGG
    GCAGGTGAAC AGCTGCCACA GATCCCAGAG CGGGTAGACA GTTTAAGCGC TCTCAGCGAC
    TCTGCAGTTG GAGTTACTGT GTCAAGGAGA CATGTGATGC CACACTCTAC ATCTTGTTCT
    GGATCTAGAA ATCGATATAA GATGGTGCCA AGCTCCACTG ACATCTTGAC AGGTCAAGGT
    CGAAAGCAGC GCAGCCTGAG CAGCACGGCA TCTCCAGCAC ACCCTGGTGG ATTTTTGGGA
    GAATGTACCA TGTACGAAGA GCCGACTATG TCAGCTACTA CAGTTGGCAG GTTCTCAGTG
    GTCAGCACAG AAGACGAGGT CACGCGTAGA AAGTACAGTA GCAGATATTC AGCTCCACCT
    GACTTTTATC TGGACGCTCC ACCTCCACTG ACCAAACGGG GGTCTTTGCC AAGGGCGCAG
    ACCTCGACTT CAGCAGATGC CACAGTTTGC ACACGATTTA TGTCATCTGA CTCTGGGGCA
    GAAAGCAGCC CTGCAAAAAT GGCACCTACA ACCCCATCCC GGCATGGCAG GTCTGAAAGG
    AGAGGAAGCG ACCTCATGAA AAGGGCAGTG GCTTTTCTAA AGCGCTCTGG CCGCAGCAGT
    AGTGTGCAAA GCTCTGATTC ACCAAGCCGG AAAGGAGGGG TGTATGGATC CTATGTCAGC
    AGTGACAATG ACTCAGAGAT GGAGGACTCA GATATTAAAA AGGAGCTTCA AAATCTAAGA
    GAAAAGCATA TGAAGGAGAT AAGTGAATTG GAGGCCCACC ACAGAGAAGA GATTGAGCTT
    CTCTATGTCA GATTAGGAAA ACCACCACCT CCAGGTCTAT ACATCCCACC CACAGTGCCC
    CCTGCTGGAC GCAAGCGCAG GACCAGCAGG CACAAACTAA AAGCTGGCAA ACTGCTCAGC
    CCTCTAGTAC AACAACTGAG AAATGTTGCT TCTAAAACTG GTGACTCCAG CAAACCTAAT
    GATTTAACCA AATCTGCTGA GGCGGTCCCG AGCTTGAATG GATCTCCTGT CAGAGCATCC
    AACTTGTCTG ATGGCAGGCC TTATTCTGGG ACCGGGACTC TGCCTTGTTC TGTTTCTGAG
    CCAGTTCAGA CCCAACAGCC CTGCTCTCTT AAAGGATCTC TGTCTTCCGA CAATATCTAC
    TATGGTGCAC AGGGAGAAGG ACCCGTGCCA CGCAGCCAGC CTGCCCAAGG CTGGCCTCCT
    TCTCCCCAGG CGTCTGCGCA GGTCACCTAT AAATCCAGTA GTAAACCACG CGCTAGATTC
    CTCAGTGGGC CAGTTTCTCT GTCCATCTGG TCAACACTTA AGCGCCTGTG TCTGGGTAAA
    GAACGTGGCA GCAGATCTGG AGCATCATCA GGTGCCTCAA ATCAATCCCC TATGCCTCCT
    GGATCCACTC CTCCTCCTCA CCAGCCTGTT GGCCTTGCCC AAGCACAGAC AAACAACAGC
    AACAACAAAA CAGATGCTTT CACTCAGCAG CTTCACCGAC TGGTGGATAA CTGGGCAGAA
    GGTGTGAGGG CTCCTTCACG CTCACGCTCC CTCAGTCTAA GACCACAGCA ACTGACCAGA
    ACCAGGATCT GGAGTGCAAC AGAGGTTCCA GGGTCAATGG AAAGATTGAA TGCTTCCCGG
    CTGCCTCTTT CATGGCCACA GATTGATTTC CATGCCTCTG TGATGGTGAC TGACACTTCA
    CAAGTGCTCC AGCACAGTTT TATGGTGTCC AGTAACCCTT ATGAAAAGAT GCTTAACTCC
    ACGCACTTTG ACAAGGACCT TTGGCCAGCA ATGACTGCCA ATCTTAACCA AGAAGTCTTT
    GCTTTCTCTG CAGCGGACTC TCCGTCCTGG ACAGCACCTT CTTCCCCCAG CGAGGGTCCT
    AGTTCTAGAA ATAGGACCAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53091
    Clone ID Related Accession (Same CDS sequence) XM_009306238.2 , XM_021479884.1 , XM_009306238.3
    Accession Version XM_009306238.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306238.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGTTTCAT CTCTATTTCC ATCCTGAAGC TTTCCTGCCT
    CAGACTTTAT CAACTCTCCA GCCACTAATG CAGGCATCCA CAGATGGTCA GTCTATTCCA
    CAGCATCTAT TACAGTCAGC ATCTTCAATG CTCAGTACCC TACCTCCACA ACCACAACTA
    AATATTCAGA CACCCCTTCT CCAGCCTCTA CAAATTTCCA CAAAGTTTCC TTCGACATAT
    CCTCTTTTAC CAGAAGGGGG CACATCTACA GGGACAGAAC CTATATCTTT CTCCTCAGTC
    TCTTACTCTT CTCCTTATCC CACAAATGCT CCTCCTGCAT CATCCCCCTA CTACTCACCA
    AGCCCCATCA GCGCTCCCCT TCCCATAATA CCCATGCAGA ATGTACCTTG CATACTAGGA
    TCAGGCACAC CTGTATCCAC CCCTCTCAAT GTTCCCACCC CTATACCTCT CCTGGCTATG
    GCCCTCTCCC CACCCATGCC TCCACCAGAG CAACATCTGC AACAGATGTT CTCCCCAATT
    CCAACACCAG AAGGTGCCCT TTACCAACCT CAACCCCAAC CAACACAGCC CTCCCTTCCC
    CTTCCAAATC CAGCATCTCT CCCTCAGCAA GACCCGTCTA TTCCTGAACC TTTTAAAGAG
    GAATACTCTC ACCATGAGGA ATTGCAGATC CTGGCTCCAT CTCACACTGT TATGTCACAG
    ATTCAGTCTC CTCCACAGGA CAGTAAGTCA GAAACAAAGT TTACCAATGT CCAAGCCTTT
    TCTGAGACAG TGGAGCATCC TCTGCAGTCT ACTGGCATTC AGAGAGCTTA TGCTGTTCCT
    GAGAGTACTG GCACAGCTAG TGCAACTCAG CAAAGGGCAG AAACTTCTGC AATGCCCAAA
    CTCCCAGCAC CAGAGCCCTG CCCATTTCAA CACAGCACTG AGGCACCTGC ATCTATTCCT
    CTCCATGCTT TTGTGTGCGA CAGCCTAAAT CAAGATGCAG GATCTAGTAA AGAAATGAGT
    GACAGCTATG AAGGATCCAC CGGTGGAGGA AAAGGTGATG GCAAACCCAG AAAGCATCAC
    CGCAAATCTG CTCGCACACG TTCACGCCAA GAAAAAATTA ACAAGCCAAA ACTAAGCATG
    TTAAATGTTA GTAATACGGG TGACAAGATG GTGGAATGCC AGTTGGAGAC ACACAATCAT
    AAAATGGTGA CTTTCAAGTT CGACCTAGAT GGAGATGCGC CTGAAGAAAT AGCCACTTAT
    ATGGTGGAAA ATGGTTTCAT TCTACCAATA GAAAAGGAGA TTTTCATAGA TCAACTAAAA
    GACATTGTTG ACAAGGCAGA GGATATTTTA AGTGAGGACA TGGATGGAGA GAAGACATCA
    GAACTTGGAA AAGGACAGAC ACCTGGAGAT AAAGGAAGAG AGGCAATAAA TGGACAGCAA
    CCTGGGATTC CCCAGGCAGT TTATCAGCAA AATGTTCTGC ACACAGGCAA GAGGTGGTTC
    ATCATCTGCC CTGTAGAAGA AGCTCCTGCA GCAAGTCAGG ATGGATGCTC AGATGGAGGT
    CCATCTACAC AATCTCCATC TACAACAACA ATAACTGAAG GAACAGCCAC TGCTCAGCCT
    GTAGAGAGTG CATCAGTACA ACCACCAGAA CCCAGCACAG GATCTGCTTC CGCTTCTATG
    GATTCAGAAA CCTGTGCCAC AGCGCCTCCA TCTGGAGGAA GTGATCCCTA TGGGGTCTGG
    AGCCCTCTCT CTTTGACAAA TACTGATCCT CTTTCTCTGG CTGCTCTTTC CCAAGCTCCT
    CCTGCCCCGC AAGCAGCTAT GATGCCAGCA CAGCCTGCTT CACATTCAGA GGAGGGCCAA
    AATTTGGGCT CTACCCAGGT CAGTGTACAG CAGTCCCAGC CCTGCATAGA TCCTCAAAGC
    TCTCTTTTTG TGGAAGAGAC TCAAAGTGGC AGATTAGACT CGGTCTCCGC AATGCACACC
    GCTCAGCAAA TGGCTGAAAT TGCATGTGCT GCCTCATTGG TGGAGGACGT GCCCTGCTGT
    CCCTTGGTCA TGCCGTTGTC CCTTGAAGTA AGCAGCGGGG CTCAGAAGCC ATCAACCGTA
    ATTCCGCCAC CATCACAAGA CAGTACATCT GCTCGCGAAC AACTCCAATC CATTACATCT
    AATCGAGCGG AGCGTGGCCT GCAACCTGTG GTGCTGCAGC AGCCTGTTTC CACTGTAAGT
    GGATCTAAGG CTCCTTCCCT GCCTCAGAGC CCTGCTCCTT CACAGCACCA CTTTGTCCCT
    GGTGAGTCAG ATGGAGAGGG GCGTAGCAGA GGAGGGTTTG TAGACAGCAC TATCAAGACT
    CTGGATGAGA AATTGAGGAA TTTATTGTAC CAAGAGTACG CTCCGATGTA CCCATCGGGA
    AGTGCAGCTG AAACTCCAGG ATCTGGTACA GAGTATATCC AGTCTCCACC AGGACCTGAG
    AGTGCCATGG GAGGGTCAGG AACCAGCACA CCTAGTCTTA TAGGAGAGGG ACGATTCAGG
    GCAGGTGAAC AGCTGCCACA GATCCCAGAG CGGGTAGACA GTTTAAGCGC TCTCAGCGAC
    TCTGCAGTTG GAGTTACTGT GTCAAGGAGA CATGTGATGC CACACTCTAC ATCTTGTTCT
    GGATCTAGAA ATCGATATAA GATGGTGCCA AGCTCCACTG ACATCTTGAC AGGTCAAGGT
    CGAAAGCAGC GCAGCCTGAG CAGCACGGCA TCTCCAGCAC ACCCTGGTGG ATTTTTGGGA
    GAATGTACCA TGTACGAAGA GCCGACTATG TCAGCTACTA CAGTTGGCAG GTTCTCAGTG
    GTCAGCACAG AAGACGAGGT CACGCGTAGA AAGTACAGTA GCAGATATTC AGCTCCACCT
    GACTTTTATC TGGACGCTCC ACCTCCACTG ACCAAACGGG GGTCTTTGCC AAGGGCGCAG
    ACCTCGACTT CAGCAGATGC CACAGTTTGC ACACGATTTA TGTCATCTGA CTCTGGGGCA
    GAAAGCAGCC CTGCAAAAAT GGCACCTACA ACCCCATCCC GGCATGGCAG GTCTGAAAGG
    AGAGGAAGCG ACCTCATGAA AAGGGCAGTG GCTTTTCTAA AGCGCTCTGG CCGCAGCAGT
    AGTGTGCAAA GCTCTGATTC ACCAAGCCGG AAAGGAGGGG TGTATGGATC CTATGTCAGC
    AGTGACAATG ACTCAGAGAT GGAGGACTCA GATATTAAAA AGGAGCTTCA AAATCTAAGA
    GAAAAGCATA TGAAGGAGAT AAGTGAATTG GAGGCCCACC ACAGAGAAGA GATTGAGCTT
    CTCTATGTCA GATTAGGAAA ACCACCACCT CCAGGTCTAT ACATCCCACC CACAGTGCCC
    CCTGCTGGAC GCAAGCGCAG GACCAGCAGG CACAAACTAA AAGCTGGCAA ACTGCTCAGC
    CCTCTAGTAC AACAACTGAG AAATGTTGCT TCTAAAACTG GTGACTCCAG CAAACCTAAT
    GATTTAACCA AATCTGCTGA GGCGGTCCCG AGCTTGAATG GATCTCCTGT CAGAGCATCC
    AACTTGTCTG ATGGCAGGCC TTATTCTGGG ACCGGGACTC TGCCTTGTTC TGTTTCTGAG
    CCAGTTCAGA CCCAACAGCC CTGCTCTCTT AAAGGATCTC TGTCTTCCGA CAATATCTAC
    TATGGTGCAC AGGGAGAAGG ACCCGTGCCA CGCAGCCAGC CTGCCCAAGG CTGGCCTCCT
    TCTCCCCAGG CGTCTGCGCA GGTCACCTAT AAATCCAGTA GTAAACCACG CGCTAGATTC
    CTCAGTGGGC CAGTTTCTCT GTCCATCTGG TCAACACTTA AGCGCCTGTG TCTGGGTAAA
    GAACGTGGCA GCAGATCTGG AGCATCATCA GGTGCCTCAA ATCAATCCCC TATGCCTCCT
    GGATCCACTC CTCCTCCTCA CCAGCCTGTT GGCCTTGCCC AAGCACAGAC AAACAACAGC
    AACAACAAAA CAGATGCTTT CACTCAGCAG CTTCACCGAC TGGTGGATAA CTGGGCAGAA
    GGTGTGAGGG CTCCTTCACG CTCACGCTCC CTCAGTCTAA GACCACAGCA ACTGACCAGA
    ACCAGGATCT GGAGTGCAAC AGAGGTTCCA GGGTCAATGG AAAGATTGAA TGCTTCCCGG
    CTGCCTCTTT CATGGCCACA GATTGATTTC CATGCCTCTG TGATGGTGAC TGACACTTCA
    CAAGTGCTCC AGCACAGTTT TATGGTGTCC AGTAACCCTT ATGAAAAGAT GCTTAACTCC
    ACGCACTTTG ACAAGGACCT TTGGCCAGCA ATGACTGCCA ATCTTAACCA AGAAGTCTTT
    GCTTTCTCTG CAGCGGACTC TCCGTCCTGG ACAGCACCTT CTTCCCCCAG CGAGGGTCCT
    AGTTCTAGAA ATAGGACCAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304513.1
    CDS692..7435
    Translation

    Target ORF information:

    RefSeq Version XM_009306238.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306238.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC CTTCTGCACA AACTGTTCCA TCTAAGACTG TCTCTGCTCC AGCTACTCCT
    GTACCGCATG CCCGTCCACA AAGCATTCCT GCTTCTGCCC TTGTAGCACA GCAAAACCAG
    ACAACTGTAC CACTAGCCCA GGAGTTTCAT CTCTATTTCC ATCCTGAAGC TTTCCTGCCT
    CAGACTTTAT CAACTCTCCA GCCACTAATG CAGGCATCCA CAGATGGTCA GTCTATTCCA
    CAGCATCTAT TACAGTCAGC ATCTTCAATG CTCAGTACCC TACCTCCACA ACCACAACTA
    AATATTCAGA CACCCCTTCT CCAGCCTCTA CAAATTTCCA CAAAGTTTCC TTCGACATAT
    CCTCTTTTAC CAGAAGGGGG CACATCTACA GGGACAGAAC CTATATCTTT CTCCTCAGTC
    TCTTACTCTT CTCCTTATCC CACAAATGCT CCTCCTGCAT CATCCCCCTA CTACTCACCA
    AGCCCCATCA GCGCTCCCCT TCCCATAATA CCCATGCAGA ATGTACCTTG CATACTAGGA
    TCAGGCACAC CTGTATCCAC CCCTCTCAAT GTTCCCACCC CTATACCTCT CCTGGCTATG
    GCCCTCTCCC CACCCATGCC TCCACCAGAG CAACATCTGC AACAGATGTT CTCCCCAATT
    CCAACACCAG AAGGTGCCCT TTACCAACCT CAACCCCAAC CAACACAGCC CTCCCTTCCC
    CTTCCAAATC CAGCATCTCT CCCTCAGCAA GACCCGTCTA TTCCTGAACC TTTTAAAGAG
    GAATACTCTC ACCATGAGGA ATTGCAGATC CTGGCTCCAT CTCACACTGT TATGTCACAG
    ATTCAGTCTC CTCCACAGGA CAGTAAGTCA GAAACAAAGT TTACCAATGT CCAAGCCTTT
    TCTGAGACAG TGGAGCATCC TCTGCAGTCT ACTGGCATTC AGAGAGCTTA TGCTGTTCCT
    GAGAGTACTG GCACAGCTAG TGCAACTCAG CAAAGGGCAG AAACTTCTGC AATGCCCAAA
    CTCCCAGCAC CAGAGCCCTG CCCATTTCAA CACAGCACTG AGGCACCTGC ATCTATTCCT
    CTCCATGCTT TTGTGTGCGA CAGCCTAAAT CAAGATGCAG GATCTAGTAA AGAAATGAGT
    GACAGCTATG AAGGATCCAC CGGTGGAGGA AAAGGTGATG GCAAACCCAG AAAGCATCAC
    CGCAAATCTG CTCGCACACG TTCACGCCAA GAAAAAATTA ACAAGCCAAA ACTAAGCATG
    TTAAATGTTA GTAATACGGG TGACAAGATG GTGGAATGCC AGTTGGAGAC ACACAATCAT
    AAAATGGTGA CTTTCAAGTT CGACCTAGAT GGAGATGCGC CTGAAGAAAT AGCCACTTAT
    ATGGTGGAAA ATGGTTTCAT TCTACCAATA GAAAAGGAGA TTTTCATAGA TCAACTAAAA
    GACATTGTTG ACAAGGCAGA GGATATTTTA AGTGAGGACA TGGATGGAGA GAAGACATCA
    GAACTTGGAA AAGGACAGAC ACCTGGAGAT AAAGGAAGAG AGGCAATAAA TGGACAGCAA
    CCTGGGATTC CCCAGGCAGT TTATCAGCAA AATGTTCTGC ACACAGGCAA GAGGTGGTTC
    ATCATCTGCC CTGTAGAAGA AGCTCCTGCA GCAAGTCAGG ATGGATGCTC AGATGGAGGT
    CCATCTACAC AATCTCCATC TACAACAACA ATAACTGAAG GAACAGCCAC TGCTCAGCCT
    GTAGAGAGTG CATCAGTACA ACCACCAGAA CCCAGCACAG GATCTGCTTC CGCTTCTATG
    GATTCAGAAA CCTGTGCCAC AGCGCCTCCA TCTGGAGGAA GTGATCCCTA TGGGGTCTGG
    AGCCCTCTCT CTTTGACAAA TACTGATCCT CTTTCTCTGG CTGCTCTTTC CCAAGCTCCT
    CCTGCCCCGC AAGCAGCTAT GATGCCAGCA CAGCCTGCTT CACATTCAGA GGAGGGCCAA
    AATTTGGGCT CTACCCAGGT CAGTGTACAG CAGTCCCAGC CCTGCATAGA TCCTCAAAGC
    TCTCTTTTTG TGGAAGAGAC TCAAAGTGGC AGATTAGACT CGGTCTCCGC AATGCACACC
    GCTCAGCAAA TGGCTGAAAT TGCATGTGCT GCCTCATTGG TGGAGGACGT GCCCTGCTGT
    CCCTTGGTCA TGCCGTTGTC CCTTGAAGTA AGCAGCGGGG CTCAGAAGCC ATCAACCGTA
    ATTCCGCCAC CATCACAAGA CAGTACATCT GCTCGCGAAC AACTCCAATC CATTACATCT
    AATCGAGCGG AGCGTGGCCT GCAACCTGTG GTGCTGCAGC AGCCTGTTTC CACTGTAAGT
    GGATCTAAGG CTCCTTCCCT GCCTCAGAGC CCTGCTCCTT CACAGCACCA CTTTGTCCCT
    GGTGAGTCAG ATGGAGAGGG GCGTAGCAGA GGAGGGTTTG TAGACAGCAC TATCAAGACT
    CTGGATGAGA AATTGAGGAA TTTATTGTAC CAAGAGTACG CTCCGATGTA CCCATCGGGA
    AGTGCAGCTG AAACTCCAGG ATCTGGTACA GAGTATATCC AGTCTCCACC AGGACCTGAG
    AGTGCCATGG GAGGGTCAGG AACCAGCACA CCTAGTCTTA TAGGAGAGGG ACGATTCAGG
    GCAGGTGAAC AGCTGCCACA GATCCCAGAG CGGGTAGACA GTTTAAGCGC TCTCAGCGAC
    TCTGCAGTTG GAGTTACTGT GTCAAGGAGA CATGTGATGC CACACTCTAC ATCTTGTTCT
    GGATCTAGAA ATCGATATAA GATGGTGCCA AGCTCCACTG ACATCTTGAC AGGTCAAGGT
    CGAAAGCAGC GCAGCCTGAG CAGCACGGCA TCTCCAGCAC ACCCTGGTGG ATTTTTGGGA
    GAATGTACCA TGTACGAAGA GCCGACTATG TCAGCTACTA CAGTTGGCAG GTTCTCAGTG
    GTCAGCACAG AAGACGAGGT CACGCGTAGA AAGTACAGTA GCAGATATTC AGCTCCACCT
    GACTTTTATC TGGACGCTCC ACCTCCACTG ACCAAACGGG GGTCTTTGCC AAGGGCGCAG
    ACCTCGACTT CAGCAGATGC CACAGTTTGC ACACGATTTA TGTCATCTGA CTCTGGGGCA
    GAAAGCAGCC CTGCAAAAAT GGCACCTACA ACCCCATCCC GGCATGGCAG GTCTGAAAGG
    AGAGGAAGCG ACCTCATGAA AAGGGCAGTG GCTTTTCTAA AGCGCTCTGG CCGCAGCAGT
    AGTGTGCAAA GCTCTGATTC ACCAAGCCGG AAAGGAGGGG TGTATGGATC CTATGTCAGC
    AGTGACAATG ACTCAGAGAT GGAGGACTCA GATATTAAAA AGGAGCTTCA AAATCTAAGA
    GAAAAGCATA TGAAGGAGAT AAGTGAATTG GAGGCCCACC ACAGAGAAGA GATTGAGCTT
    CTCTATGTCA GATTAGGAAA ACCACCACCT CCAGGTCTAT ACATCCCACC CACAGTGCCC
    CCTGCTGGAC GCAAGCGCAG GACCAGCAGG CACAAACTAA AAGCTGGCAA ACTGCTCAGC
    CCTCTAGTAC AACAACTGAG AAATGTTGCT TCTAAAACTG GTGACTCCAG CAAACCTAAT
    GATTTAACCA AATCTGCTGA GGCGGTCCCG AGCTTGAATG GATCTCCTGT CAGAGCATCC
    AACTTGTCTG ATGGCAGGCC TTATTCTGGG ACCGGGACTC TGCCTTGTTC TGTTTCTGAG
    CCAGTTCAGA CCCAACAGCC CTGCTCTCTT AAAGGATCTC TGTCTTCCGA CAATATCTAC
    TATGGTGCAC AGGGAGAAGG ACCCGTGCCA CGCAGCCAGC CTGCCCAAGG CTGGCCTCCT
    TCTCCCCAGG CGTCTGCGCA GGTCACCTAT AAATCCAGTA GTAAACCACG CGCTAGATTC
    CTCAGTGGGC CAGTTTCTCT GTCCATCTGG TCAACACTTA AGCGCCTGTG TCTGGGTAAA
    GAACGTGGCA GCAGATCTGG AGCATCATCA GGTGCCTCAA ATCAATCCCC TATGCCTCCT
    GGATCCACTC CTCCTCCTCA CCAGCCTGTT GGCCTTGCCC AAGCACAGAC AAACAACAGC
    AACAACAAAA CAGATGCTTT CACTCAGCAG CTTCACCGAC TGGTGGATAA CTGGGCAGAA
    GGTGTGAGGG CTCCTTCACG CTCACGCTCC CTCAGTCTAA GACCACAGCA ACTGACCAGA
    ACCAGGATCT GGAGTGCAAC AGAGGTTCCA GGGTCAATGG AAAGATTGAA TGCTTCCCGG
    CTGCCTCTTT CATGGCCACA GATTGATTTC CATGCCTCTG TGATGGTGAC TGACACTTCA
    CAAGTGCTCC AGCACAGTTT TATGGTGTCC AGTAACCCTT ATGAAAAGAT GCTTAACTCC
    ACGCACTTTG ACAAGGACCT TTGGCCAGCA ATGACTGCCA ATCTTAACCA AGAAGTCTTT
    GCTTTCTCTG CAGCGGACTC TCCGTCCTGG ACAGCACCTT CTTCCCCCAG CGAGGGTCCT
    AGTTCTAGAA ATAGGACCAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54383
    Clone ID Related Accession (Same CDS sequence) XM_017358364.1
    Accession Version XM_017358364.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
    181
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC GACATGGCAG TATTCCTGGT GACTCCCAAG GAATATTCTC TAATAACTTG
    GCGGGGACCA GGCGCAGCAG CACATCATTC CTCGACACAT TACAGAGAAG GGCCGACCTA
    CAAGATTTTT TAGAACGCCT TCATTATCAG GCATATAGCT GTCCCAAACC TGGAGCAAGT
    TCTAGATATA AAATAAATCA GTCGCAGTCT ACAAGTGCCT TTGTAGGGTC TTCATCATTC
    ACAAATGATG AGCAGGAAAC AGATATTTCT AAAGACATTG GAGACTTAGG ACATAGATCA
    GAACAAGTAA AAATTCATTC TCAGTCAATG TCATTCGGTC GAAGGAATTC TGATATCCCC
    CCACATTACT GCCAGGCTTG TAGGGCAATG TTGCTGGGTG TAAGGTCAGG GGGTCGAGGG
    TCTCTTGGCC ATGCTTCTTC CTCCCATATC CACCAAAATA AGTCCACTGT ATTCCCAAAT
    AAACCACCTT TGTCACCTGA TCCGGCTCAT CTAGATTCAA TTTATATGAC TCAACAGTCT
    CCACATATCC CTCCAAAGCA CGTTTCTGTT CCTAAGAACA TATCAACTTC AATACCAGTT
    CAAGTCACCC CACCCTCTCC TGGACACGAA GGCCATCCTC CTGCTCAGGT TCCTGTCACA
    GGATTAAATC ATAGCACTGC CTTCTCCCCT GTCTATATAA CAATACCCTC TCCAGTCCAC
    ACTTCTTCCA GACATACATC TATATCTTCT CCAGTTGATT TAGTCTCCCC CCTCTGGCCA
    TCCTCTCCAG TGTCCCCTGT CTCCTTGATT GGGCCACATT CTCCTAGTTT GGGAGACAGC
    TCAGATTTGT CTCACCTTGA CCGCTGTCTC CATCATATCA TCAATCGTCG TACTAGCTCC
    CCTGTGCTAG CTGACAGGGC ACAGCCTGGA GATGGCCACG GCGATTCTTC TATTAACAGG
    TTTCTTGGAC TTCAGTTCCA AGCCAAAAGC TCTTTGTGTG TACCTCTATC ACATCAGGAA
    ACTTTTTCTG TGCAGGACAG TGAGGTTGGA CCAGTTCTTG TGCCTTCTGC ACAAACTGTT
    CCATCTAAGA CTGTCTCTGC TCCAGCTACT CCTGTACCGC ATGCCCGTCC ACAAAGCATT
    CCTGCTTCTG CCCTTGTAGC ACAGCAAAAC CAGACAACTG TACCACTAGC CCAGGAGTTT
    CATCTCTATT TCCATCCTGA AGCTTTCCTG CCTCAGACTT TATCAACTCT CCAGCCACTA
    ATGCAGGCAT CCACAGATGG TCAGTCTATT CCACAGCATC TATTACAGTC AGCATCTTCA
    ATGCTCAGTA CCCTACCTCC ACAACCACAA CTAAATATTC AGACACCCCT TCTCCAGCCT
    CTACAAATTT CCACAAAGTT TCCTTCGACA TATCCTCTTT TACCAGAAGG GGGCACATCT
    ACAGGGACAG AACCTATATC TTTCTCCTCA GTCTCTTACT CTTCTCCTTA TCCCACAAAT
    GCTCCTCCTG CATCATCCCC CTACTACTCA CCAAGCCCCA TCAGCGCTCC CCTTCCCATA
    ATACCCATGC AGAATGTACC TTGCATACTA GGATCAGGCA CACCTGTATC CACCCCTCTC
    AATGTTCCCA CCCCTATACC TCTCCTGGCT ATGGCCCTCT CCCCACCCAT GCCTCCACCA
    GAGCAACATC TGCAACAGAT GTTCTCCCCA ATTCCAACAC CAGAAGGTGC CCTTTACCAA
    CCTCAACCCC AACCAACACA GCCCTCCCTT CCCCTTCCAA ATCCAGCATC TCTCCCTCAG
    CAAGACCCGT CTATTCCTGA ACCTTTTAAA GAGGAATACT CTCACCATGA GGAATTGCAG
    ATCCTGGCTC CATCTCACAC TGTTATGTCA CAGATTCAGT CTCCTCCACA GGACAGTAAG
    TCAGAAACAA AGTTTACCAA TGTCCAAGCC TTTTCTGAGA CAGTGGAGCA TCCTCTGCAG
    TCTACTGGCA TTCAGAGAGC TTATGCTGTT CCTGAGAGTA CTGGCACAGC TAGTGCAACT
    CAGCAAAGGG CAGAAACTTC TGCAATGCCC AAACTCCCAG CACCAGAGCC CTGCCCATTT
    CAACACAGCA CTGAGGCACC TGCATCTATT CCTCTCCATG CTTTTGTGTG CGACAGCCTA
    AATCAAGATG CAGGATCTAG TAAAGAAATG AGTGACAGCT ATGAAGGATC CACCGGTGGA
    GGAAAAGGTG ATGGCAAACC CAGAAAGCAT CACCGCAAAT CTGCTCGCAC ACGTTCACGC
    CAAGAAAAAA TTAACAAGCC AAAACTAAGC ATGTTAAATG TTAGTAATAC GGGTGACAAG
    ATGGTGGAAT GCCAGTTGGA GACACACAAT CATAAAATGG TGACTTTCAA GTTCGACCTA
    GATGGAGATG CGCCTGAAGA AATAGCCACT TATATGGTGG AAAATGGTTT CATTCTACCA
    ATAGAAAAGG AGATTTTCAT AGATCAACTA AAAGACATTG TTGACAAGGC AGAGGATATT
    TTAAGTGAGG ACATGGATGG AGAGAAGACA TCAGAACTTG GAAAAGGACA GACACCTGGA
    GATAAAGGAA GAGAGGCAAT AAATGGACAG CAACCTGGGA TTCCCCAGGC AGTTTATCAG
    CAAAATGTTC TGCACACAGG CAAGAGGTGG TTCATCATCT GCCCTGTAGA AGAAGCTCCT
    GCAGCAAGTC AGGATGGATG CTCAGATGGA GGTCCATCTA CACAATCTCC ATCTACAACA
    ACAATAACTG AAGGAACAGC CACTGCTCAG CCTGTAGAGA GTGCATCAGT ACAACCACCA
    GAACCCAGCA CAGGATCTGC TTCCGCTTCT ATGGATTCAG AAACCTGTGC CACAGCGCCT
    CCATCTGGAG GAAGTGATCC CTATGGGGTC TGGAGCCCTC TCTCTTTGAC AAATACTGAT
    CCTCTTTCTC TGGCTGCTCT TTCCCAAGCT CCTCCTGCCC CGCAAGCAGC TATGATGCCA
    GCACAGCCTG CTTCACATTC AGAGGAGGGC CAAAATTTGG GCTCTACCCA GGTCAGTGTA
    CAGCAGTCCC AGCCCTGCAT AGATCCTCAA AGCTCTCTTT TTGTGGAAGA GACTCAAAGT
    GGCAGATTAG ACTCGGTCTC CGCAATGCAC ACCGCTCAGC AAATGGCTGA AATTGCATGT
    GCTGCCTCAT TGGTGGAGGA CGTGCCCTGC TGTCCCTTGG TCATGCCGTT GTCCCTTGAA
    GTAAGCAGCG GGGCTCAGAA GCCATCAACC GTAATTCCGC CACCATCACA AGACAGTACA
    TCTGCTCGCG AACAACTCCA ATCCATTACA TCTAATCGAG CGGAGCGTGG CCTGCAACCT
    GTGGTGCTGC AGCAGCCTGT TTCCACTGTA AGTGGATCTA AGGCTCCTTC CCTGCCTCAG
    AGCCCTGCTC CTTCACAGCA CCACTTTGTC CCTGGTGAGT CAGATGGAGA GGGGCGTAGC
    AGAGGAGGGT TTGTAGACAG CACTATCAAG ACTCTGGATG AGAAATTGAG GAATTTATTG
    TACCAAGAGT ACGCTCCGAT GTACCCATCG GGAAGTGCAG CTGAAACTCC AGGATCTGGT
    ACAGAGTATA TCCAGTCTCC ACCAGGACCT GAGAGTGCCA TGGGAGGGTC AGGAACCAGC
    ACACCTAGTC TTATAGGAGA GGGACGATTC AGGGCAGGTG AACAGCTGCC ACAGATCCCA
    GAGCGGGTAG ACAGTTTAAG CGCTCTCAGC GACTCTGCAG TTGGAGTTAC TGTGTCAAGG
    AGACATGTGA TGCCACACTC TACATCTTGT TCTGGATCTA GAAATCGATA TAAGATGGTG
    CCAAGCTCCA CTGACATCTT GACAGGTCAA GGTCGAAAGC AGCGCAGCCT GAGCAGCACG
    GCATCTCCAG CACACCCTGG TGGATTTTTG GGAGAATGTA CCATGTACGA AGAGCCGACT
    ATGTCAGCTA CTACAGTTGG CAGGTTCTCA GTGGTCAGCA CAGAAGACGA GGTCACGCGT
    AGAAAGTACA GTAGCAGATA TTCAGCTCCA CCTGACTTTT ATCTGGACGC TCCACCTCCA
    CTGACCAAAC GGGGGTCTTT GCCAAGGGCG CAGACCTCGA CTTCAGCAGA TGCCACAGTT
    TGCACACGAT TTATGTCATC TGACTCTGGG GCAGAAAGCA GCCCTGCAAA AATGGCACCT
    ACAACCCCAT CCCGGCATGG CAGGTCTGAA AGGAGAGGAA GCGACCTCAT GAAAAGGGCA
    GTGGCTTTTC TAAAGCGCTC TGGCCGCAGC AGTAGTGTGC AAAGCTCTGA TTCACCAAGC
    CGGAAAGGAG GGGTGTATGG ATCCTATGTC AGCAGTGACA ATGACTCAGA GATGGAGGAC
    TCAGATATTA AAAAGGAGCT TCAAAATCTA AGAGAAAAGC ATATGAAGGA GATAAGTGAA
    TTGGAGGCCC ACCACAGAGA AGAGATTGAG CTTCTCTATG TCAGATTAGG AAAACCACCA
    CCTCCAGGTC TATACATCCC ACCCACAGTG CCCCCTGCTG GACGCAAGCG CAGGACCAGC
    AGGCACAAAC TAAAAGCTGG CAAACTGCTC AGCCCTCTAG TACAACAACT GAGAAATGTT
    GCTTCTAAAA CTGGTGACTC CAGCAAACCT AATGATTTAA CCAAATCTGC TGAGGCGGTC
    CCGAGCTTGA ATGGATCTCC TGTCAGAGCA TCCAACTTGT CTGATGGCAG GCCTTATTCT
    GGGACCGGGA CTCTGCCTTG TTCTGTTTCT GAGCCAGTTC AGACCCAACA GCCCTGCTCT
    CTTAAAGGAT CTCTGTCTTC CGACAATATC TACTATGGTG CACAGGGAGA AGGACCCGTG
    CCACGCAGCC AGCCTGCCCA AGGCTGGCCT CCTTCTCCCC AGGCGTCTGC GCAGGTCACC
    TATAAATCCA GTAGTAAACC ACGCGCTAGA TTCCTCAGTG GGCCAGTTTC TCTGTCCATC
    TGGTCAACAC TTAAGCGCCT GTGTCTGGGT AAAGAACGTG GCAGCAGATC TGGAGCATCA
    TCAGGTGCCT CAAATCAATC CCCTATGCCT CCTGGATCCA CTCCTCCTCC TCACCAGCCT
    GTTGGCCTTG CCCAAGCACA GACAAACAAC AGCAACAACA AAACAGATGC TTTCACTCAG
    CAGCTTCACC GACTGGTGGA TAACTGGGCA GAAGGTGTGA GGGCTCCTTC ACGCTCACGC
    TCCCTCAGTC TAAGACCACA GCAACTGACC AGAACCAGGA TCTGGAGTGC AACAGAGGTT
    CCAGGGTCAA TGGAAAGATT GAATGCTTCC CGGCTGCCTC TTTCATGGCC ACAGATTGAT
    TTCCATGCCT CTGTGATGGT GACTGACACT TCACAAGTGC TCCAGCACAG TTTTATGGTG
    TCCAGTAACC CTTATGAAAA GATGCTTAAC TCCACGCACT TTGACAAGGA CCTTTGGCCA
    GCAATGACTG CCAATCTTAA CCAAGAAGTC TTTGCTTTCT CTGCAGCGGA CTCTCCGTCC
    TGGACAGCAC CTTCTTCCCC CAGCGAGGGT CCTAGTTCTA GAAATAGGAC CATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213853.1
    CDS712..8508
    Translation

    Target ORF information:

    RefSeq Version XM_017358364.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio WNK lysine deficient protein kinase 2 (wnk2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGAAAACC TGACTGGATC CAGCGCTGAG CAGCAGCCTG TGGGCTCCAC AGGTCCTTCT 
    GTTCCCTCAA AAAAGACTGT AATTTATGAA AATGGTCATG CACATCCCAC TCATACAGAC
    CCTGCAGCTG CAGCACACAG AGGGGCCAGC GATCCAACTG CATACCCCCT ATCAGATTAC
    CGAGGCCTGG TCCGGCAGAG ATTTATACGC AGAAGTTTGT GGTTCTCTGA GTCAGAGGAT
    CAAGAACTCG AGGTTGCAGA GTGCGACATC ACCAGCAGCC CTGTTAAAAG TGCACATATC
    GCCATCCAGA GGAGCTTTAA CAAGGAGAGT CAAGTGGGAC AGGCAGACGG TACAAACGAG
    AACTTGAGCA AAGAGCCAGA GACAAGTGAG ACCGAGGAAA AGCAAGATGC TGAGCAGGCT
    GAAGCTGCGG GGAAAGCTGG GAGTGATGAG AACGAGGAAG AGGCTGAAAT GAAGGCGGTT
    TCCACTTCTC CAGGTGGACG CTTTCTTAAG TTTGATATTG AGCTTGGCAG AGGCTCCTTC
    AAAACTGTTT ATAAGGGCCT GGACACTGAG ACCTGGGTGG AGGTGGCCTG GTGTGAACTT
    CAGGACCGAA AACTTTCCAA AGCAGAACGT CAGAGGTTTA AAGAGGAGGC AGAGATGCTA
    AAAGGCCTTC AGCATCCCAA CATTGTGCGC TTCTATGACT TTTGGGAATC TCCTCTCAAA
    GGAAAGAAGT GCATTGTTTT AGTCACTGAA CTGATGACAT CTGGAACCCT GAAAACGTAT
    CTAAAGCGAT TTAAGGTGAT GAAACCTAAA GTTTTAAGAA GTTGGTGCAG ACAGATCTTA
    AAAGGTCTTC ACTTCCTCCA TACGAGGACT CCTCCTATTA TCCACCGGGA CCTGAAGTGT
    GACAATATCT TCATCACTGG TCCTACAGGC TCAGTCAAGA TTGGAGATCT TGGCCTGGCC
    ACACTAAAGA GAGCTTCCTT TGCCAAAAGT GTAATAGGCA CACCAGAATT TATGGCCCCT
    GAGATGTACG AGGAGCACTA CGATGAAGCT GTAGATGTAT ATGCATTTGG GATGTGCATG
    CTAGAAATGG CCACATCTGA ATATCCATAC TCAGAGTGCC AAAATGCAGC ACAAATATAT
    CGCAAAGTCA CTAGCGGTGT GAAGCCAGCC AGTTTCTGTA AGGTGGCTGT GCCTGAAATA
    AAAGAAATCA TTGGAGAGTG TATTTGCCAT CGCTGGGAGG AAAGGTACTC CATAAAGGAC
    TTACTAAACC ATGCATTTTT TGCTGAAGAC ACTGGGGTGA GAGTAGAGCT GGCTGAAGAG
    GATGATGGCA AAAAGTCTTC CATCGCTCTG AGGCTTTGGG TTGAAGACCA GAAGAAACTC
    AAGGGGAAAT ATAAAGACAG TGGTGCTATT GAGTTCAGCT TTGACCTGGA GACAGAAGTC
    CCGGAGTCAA TCGCCCAGGA AATGGTGGAC TCTGGCTTCT TTTTAGAGAT TGATGTGAAG
    ATTGTGAGTA AGTCTATCCG TGACCGGGTG TCTCTAATAA AGTGGAGACG ACAACGGATT
    GTCTCAGGAT GTAATGGAAA AACCGAAGAG TGCAGCACCA AGACTGAGAC ACAGCATCTC
    CTTCAGGTGC CCTCCACGGG GCCACCAGTC GGTGGTACAC CCAGTCTTCC ATCCGAAACA
    GAGGAGGTAC AGACTGAAGC AGTCAGCATG GTCTGCAGTG TCCCAACCAG TGAGACTACA
    GCTACACCAG ACAGCAGTGC AGGCTCAACA ATGGTAACAG GAGCTTCGGG CAACCAAGAC
    AGCACCTCTC AGCAGTCCCT TTCGGAGTCC ATTTCCACTG CCCAAAGGGT CTTAAGCCCT
    CCAGCACAGC TTCTGACTCA GTTTCCACAG GCTGGCCTGC TGACATCTAC TGCACATCTG
    CCTCTGGGGA CTCAACAACA ACCTGTTCTA CAAATGCACC AAGGTGTCAC TCAGCAAACA
    ATAGACCATT TGTTCCATGG AGCCCAGCAA CAACCTAGTC CTCGGCAACC CCAGGGAACC
    CAACAACAAC AACCTAGTGC CCCACTGCAC CAGGCTGCAC AAGCACATGG ATTGATGTCT
    CAAGCACCCC AGCAACAACC CACCGTTCAA CTGCACCAGC AATATCAGCA GGCAACTCAT
    CAGCTACATC AAGGGGCTTT TCAGCAATCC TCAGTACATC TGCATCAGAG CGCCTACCAG
    CAGTCACCTC GACATGGCAG TATTCCTGGT GACTCCCAAG GAATATTCTC TAATAACTTG
    GCGGGGACCA GGCGCAGCAG CACATCATTC CTCGACACAT TACAGAGAAG GGCCGACCTA
    CAAGATTTTT TAGAACGCCT TCATTATCAG GCATATAGCT GTCCCAAACC TGGAGCAAGT
    TCTAGATATA AAATAAATCA GTCGCAGTCT ACAAGTGCCT TTGTAGGGTC TTCATCATTC
    ACAAATGATG AGCAGGAAAC AGATATTTCT AAAGACATTG GAGACTTAGG ACATAGATCA
    GAACAAGTAA AAATTCATTC TCAGTCAATG TCATTCGGTC GAAGGAATTC TGATATCCCC
    CCACATTACT GCCAGGCTTG TAGGGCAATG TTGCTGGGTG TAAGGTCAGG GGGTCGAGGG
    TCTCTTGGCC ATGCTTCTTC CTCCCATATC CACCAAAATA AGTCCACTGT ATTCCCAAAT
    AAACCACCTT TGTCACCTGA TCCGGCTCAT CTAGATTCAA TTTATATGAC TCAACAGTCT
    CCACATATCC CTCCAAAGCA CGTTTCTGTT CCTAAGAACA TATCAACTTC AATACCAGTT
    CAAGTCACCC CACCCTCTCC TGGACACGAA GGCCATCCTC CTGCTCAGGT TCCTGTCACA
    GGATTAAATC ATAGCACTGC CTTCTCCCCT GTCTATATAA CAATACCCTC TCCAGTCCAC
    ACTTCTTCCA GACATACATC TATATCTTCT CCAGTTGATT TAGTCTCCCC CCTCTGGCCA
    TCCTCTCCAG TGTCCCCTGT CTCCTTGATT GGGCCACATT CTCCTAGTTT GGGAGACAGC
    TCAGATTTGT CTCACCTTGA CCGCTGTCTC CATCATATCA TCAATCGTCG TACTAGCTCC
    CCTGTGCTAG CTGACAGGGC ACAGCCTGGA GATGGCCACG GCGATTCTTC TATTAACAGG
    TTTCTTGGAC TTCAGTTCCA AGCCAAAAGC TCTTTGTGTG TACCTCTATC ACATCAGGAA
    ACTTTTTCTG TGCAGGACAG TGAGGTTGGA CCAGTTCTTG TGCCTTCTGC ACAAACTGTT
    CCATCTAAGA CTGTCTCTGC TCCAGCTACT CCTGTACCGC ATGCCCGTCC ACAAAGCATT
    CCTGCTTCTG CCCTTGTAGC ACAGCAAAAC CAGACAACTG TACCACTAGC CCAGGAGTTT
    CATCTCTATT TCCATCCTGA AGCTTTCCTG CCTCAGACTT TATCAACTCT CCAGCCACTA
    ATGCAGGCAT CCACAGATGG TCAGTCTATT CCACAGCATC TATTACAGTC AGCATCTTCA
    ATGCTCAGTA CCCTACCTCC ACAACCACAA CTAAATATTC AGACACCCCT TCTCCAGCCT
    CTACAAATTT CCACAAAGTT TCCTTCGACA TATCCTCTTT TACCAGAAGG GGGCACATCT
    ACAGGGACAG AACCTATATC TTTCTCCTCA GTCTCTTACT CTTCTCCTTA TCCCACAAAT
    GCTCCTCCTG CATCATCCCC CTACTACTCA CCAAGCCCCA TCAGCGCTCC CCTTCCCATA
    ATACCCATGC AGAATGTACC TTGCATACTA GGATCAGGCA CACCTGTATC CACCCCTCTC
    AATGTTCCCA CCCCTATACC TCTCCTGGCT ATGGCCCTCT CCCCACCCAT GCCTCCACCA
    GAGCAACATC TGCAACAGAT GTTCTCCCCA ATTCCAACAC CAGAAGGTGC CCTTTACCAA
    CCTCAACCCC AACCAACACA GCCCTCCCTT CCCCTTCCAA ATCCAGCATC TCTCCCTCAG
    CAAGACCCGT CTATTCCTGA ACCTTTTAAA GAGGAATACT CTCACCATGA GGAATTGCAG
    ATCCTGGCTC CATCTCACAC TGTTATGTCA CAGATTCAGT CTCCTCCACA GGACAGTAAG
    TCAGAAACAA AGTTTACCAA TGTCCAAGCC TTTTCTGAGA CAGTGGAGCA TCCTCTGCAG
    TCTACTGGCA TTCAGAGAGC TTATGCTGTT CCTGAGAGTA CTGGCACAGC TAGTGCAACT
    CAGCAAAGGG CAGAAACTTC TGCAATGCCC AAACTCCCAG CACCAGAGCC CTGCCCATTT
    CAACACAGCA CTGAGGCACC TGCATCTATT CCTCTCCATG CTTTTGTGTG CGACAGCCTA
    AATCAAGATG CAGGATCTAG TAAAGAAATG AGTGACAGCT ATGAAGGATC CACCGGTGGA
    GGAAAAGGTG ATGGCAAACC CAGAAAGCAT CACCGCAAAT CTGCTCGCAC ACGTTCACGC
    CAAGAAAAAA TTAACAAGCC AAAACTAAGC ATGTTAAATG TTAGTAATAC GGGTGACAAG
    ATGGTGGAAT GCCAGTTGGA GACACACAAT CATAAAATGG TGACTTTCAA GTTCGACCTA
    GATGGAGATG CGCCTGAAGA AATAGCCACT TATATGGTGG AAAATGGTTT CATTCTACCA
    ATAGAAAAGG AGATTTTCAT AGATCAACTA AAAGACATTG TTGACAAGGC AGAGGATATT
    TTAAGTGAGG ACATGGATGG AGAGAAGACA TCAGAACTTG GAAAAGGACA GACACCTGGA
    GATAAAGGAA GAGAGGCAAT AAATGGACAG CAACCTGGGA TTCCCCAGGC AGTTTATCAG
    CAAAATGTTC TGCACACAGG CAAGAGGTGG TTCATCATCT GCCCTGTAGA AGAAGCTCCT
    GCAGCAAGTC AGGATGGATG CTCAGATGGA GGTCCATCTA CACAATCTCC ATCTACAACA
    ACAATAACTG AAGGAACAGC CACTGCTCAG CCTGTAGAGA GTGCATCAGT ACAACCACCA
    GAACCCAGCA CAGGATCTGC TTCCGCTTCT ATGGATTCAG AAACCTGTGC CACAGCGCCT
    CCATCTGGAG GAAGTGATCC CTATGGGGTC TGGAGCCCTC TCTCTTTGAC AAATACTGAT
    CCTCTTTCTC TGGCTGCTCT TTCCCAAGCT CCTCCTGCCC CGCAAGCAGC TATGATGCCA
    GCACAGCCTG CTTCACATTC AGAGGAGGGC CAAAATTTGG GCTCTACCCA GGTCAGTGTA
    CAGCAGTCCC AGCCCTGCAT AGATCCTCAA AGCTCTCTTT TTGTGGAAGA GACTCAAAGT
    GGCAGATTAG ACTCGGTCTC CGCAATGCAC ACCGCTCAGC AAATGGCTGA AATTGCATGT
    GCTGCCTCAT TGGTGGAGGA CGTGCCCTGC TGTCCCTTGG TCATGCCGTT GTCCCTTGAA
    GTAAGCAGCG GGGCTCAGAA GCCATCAACC GTAATTCCGC CACCATCACA AGACAGTACA
    TCTGCTCGCG AACAACTCCA ATCCATTACA TCTAATCGAG CGGAGCGTGG CCTGCAACCT
    GTGGTGCTGC AGCAGCCTGT TTCCACTGTA AGTGGATCTA AGGCTCCTTC CCTGCCTCAG
    AGCCCTGCTC CTTCACAGCA CCACTTTGTC CCTGGTGAGT CAGATGGAGA GGGGCGTAGC
    AGAGGAGGGT TTGTAGACAG CACTATCAAG ACTCTGGATG AGAAATTGAG GAATTTATTG
    TACCAAGAGT ACGCTCCGAT GTACCCATCG GGAAGTGCAG CTGAAACTCC AGGATCTGGT
    ACAGAGTATA TCCAGTCTCC ACCAGGACCT GAGAGTGCCA TGGGAGGGTC AGGAACCAGC
    ACACCTAGTC TTATAGGAGA GGGACGATTC AGGGCAGGTG AACAGCTGCC ACAGATCCCA
    GAGCGGGTAG ACAGTTTAAG CGCTCTCAGC GACTCTGCAG TTGGAGTTAC TGTGTCAAGG
    AGACATGTGA TGCCACACTC TACATCTTGT TCTGGATCTA GAAATCGATA TAAGATGGTG
    CCAAGCTCCA CTGACATCTT GACAGGTCAA GGTCGAAAGC AGCGCAGCCT GAGCAGCACG
    GCATCTCCAG CACACCCTGG TGGATTTTTG GGAGAATGTA CCATGTACGA AGAGCCGACT
    ATGTCAGCTA CTACAGTTGG CAGGTTCTCA GTGGTCAGCA CAGAAGACGA GGTCACGCGT
    AGAAAGTACA GTAGCAGATA TTCAGCTCCA CCTGACTTTT ATCTGGACGC TCCACCTCCA
    CTGACCAAAC GGGGGTCTTT GCCAAGGGCG CAGACCTCGA CTTCAGCAGA TGCCACAGTT
    TGCACACGAT TTATGTCATC TGACTCTGGG GCAGAAAGCA GCCCTGCAAA AATGGCACCT
    ACAACCCCAT CCCGGCATGG CAGGTCTGAA AGGAGAGGAA GCGACCTCAT GAAAAGGGCA
    GTGGCTTTTC TAAAGCGCTC TGGCCGCAGC AGTAGTGTGC AAAGCTCTGA TTCACCAAGC
    CGGAAAGGAG GGGTGTATGG ATCCTATGTC AGCAGTGACA ATGACTCAGA GATGGAGGAC
    TCAGATATTA AAAAGGAGCT TCAAAATCTA AGAGAAAAGC ATATGAAGGA GATAAGTGAA
    TTGGAGGCCC ACCACAGAGA AGAGATTGAG CTTCTCTATG TCAGATTAGG AAAACCACCA
    CCTCCAGGTC TATACATCCC ACCCACAGTG CCCCCTGCTG GACGCAAGCG CAGGACCAGC
    AGGCACAAAC TAAAAGCTGG CAAACTGCTC AGCCCTCTAG TACAACAACT GAGAAATGTT
    GCTTCTAAAA CTGGTGACTC CAGCAAACCT AATGATTTAA CCAAATCTGC TGAGGCGGTC
    CCGAGCTTGA ATGGATCTCC TGTCAGAGCA TCCAACTTGT CTGATGGCAG GCCTTATTCT
    GGGACCGGGA CTCTGCCTTG TTCTGTTTCT GAGCCAGTTC AGACCCAACA GCCCTGCTCT
    CTTAAAGGAT CTCTGTCTTC CGACAATATC TACTATGGTG CACAGGGAGA AGGACCCGTG
    CCACGCAGCC AGCCTGCCCA AGGCTGGCCT CCTTCTCCCC AGGCGTCTGC GCAGGTCACC
    TATAAATCCA GTAGTAAACC ACGCGCTAGA TTCCTCAGTG GGCCAGTTTC TCTGTCCATC
    TGGTCAACAC TTAAGCGCCT GTGTCTGGGT AAAGAACGTG GCAGCAGATC TGGAGCATCA
    TCAGGTGCCT CAAATCAATC CCCTATGCCT CCTGGATCCA CTCCTCCTCC TCACCAGCCT
    GTTGGCCTTG CCCAAGCACA GACAAACAAC AGCAACAACA AAACAGATGC TTTCACTCAG
    CAGCTTCACC GACTGGTGGA TAACTGGGCA GAAGGTGTGA GGGCTCCTTC ACGCTCACGC
    TCCCTCAGTC TAAGACCACA GCAACTGACC AGAACCAGGA TCTGGAGTGC AACAGAGGTT
    CCAGGGTCAA TGGAAAGATT GAATGCTTCC CGGCTGCCTC TTTCATGGCC ACAGATTGAT
    TTCCATGCCT CTGTGATGGT GACTGACACT TCACAAGTGC TCCAGCACAG TTTTATGGTG
    TCCAGTAACC CTTATGAAAA GATGCTTAAC TCCACGCACT TTGACAAGGA CCTTTGGCCA
    GCAATGACTG CCAATCTTAA CCAAGAAGTC TTTGCTTTCT CTGCAGCGGA CTCTCCGTCC
    TGGACAGCAC CTTCTTCCCC CAGCGAGGGT CCTAGTTCTA GAAATAGGAC CATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.