cntrob cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cntrob
Entrez Gene ID	101885447
Full Name	centrobin, centriole duplication and spindle assembly protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021470054.1	XP_021325729.1	centrobin isoform X1
XM_021470055.1	XP_021325730.1	centrobin isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cntrob gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cntrob cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa151620	XM_021470054.1 Latest version!	Danio rerio centrobin, centriole duplication and spindle assembly protein (cntrob), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa151621	XM_021470055.1 Latest version!	Danio rerio centrobin, centriole duplication and spindle assembly protein (cntrob), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cntrob ( XM_021470054.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325729.1 Danio rerio centrobin, centriole duplication and spindle assembly protein (cntrob), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa151620
Clone ID Related Accession (Same CDS sequence)	XM_021470054.1
Accession Version	XM_021470054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrobin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) and transcript sequence (GFIL01004366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1108 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCTGTGA GCCGAGCTGC TGCTGATGGT GTTAGTGATG GTGGTCTGGT GCGCTCATGG 
CCCTCGTCTC CTCTGTCTGC GACTCGTCAG GTCACCGCTC GCCTCTACAG CTCATTACAG 
CACAGCCGAC AGCAGGAGGT CAAAGGTCAC GCTGCAGACA GGCAGGTGTC ATTCGCTCTG 
TCCTCTCCTG ATCTCACCGC TGTCAGGACG ACCTCAGCCA CGCCTCCTCT GCTCTCTGAT 
AGGCTGGAGG AGCTCAGTTT GGACTCCGCC TCCTCTCATG ATGTGATGGG CATAGGAGAG 
GAGGGGGTGG AGTCAGATGC AGAAGAGGTG GGGCTTCAAC TTTTGAAGAG CCTGAGATCT 
GCACAACCGA CGAGTGGCAG GAAGCACATA GAGGAGATGG AAAGTGTGAG GACACACTTG 
CAGTCTATAC TGAGGCCAAC ACAGACAACT GCAGACAGAC ACGATTTTCT GACTCCTGCA 
TCACAGCACT TCCTGGATGA TTCACATGAG AGTGATGCCA CGTCACGCCT GCTCAGTGGT 
GTGTGTGTAG GTGGTGTGGA GGAGTTGTTC CCTCGTTATT CCCGTCTTCA TTCAGATGTG 
AGCTGCGCAG TTTCTGAGCT GCAGGTGCTG AGAGACAGTC TGGAGAGAGA GCGAGAGCGC 
CGCAAGGTGT GTGAGCAGCA GGTGGCGTCT CTCCAAAGTA AAGTTCTTCA GTTTCAGCAG 
CAGTTAACAC TCGCTGTAGC CGCCGACCGC AAGAAAGACA TCATGATTGA ACAGTTGGAC 
AAGACGCTGG TGAAGGTGGT TGAGGGCTGG AAGAGGCACG ATCAGGAGCG AAATGAGGAG 
ATGAAACGGC TGCAGGAGGA GAAGGAGACC GCTGAGAAAA CACACAACAA ACATGCACAG 
GCTCTGTCTC GTGTTGAGCA GAATCTCTCT GAGGTTGAGG AAACTCTGAA GAAGGAGCAG 
AAACACAATC AGGAGCTGCA GAAGACCAAC AAACGACTGG AGCAAGAGGC GTCTGATCTG 
CGTGTGCATG TGGAGGACTT ACAGCAAGAC AAGCAAAAGC TTAGCAGAGA AGCAGATAAA 
CACAGAGAGC AGCTCCACAA ACTCCAGACA GAGTCGCATG ACACACGGAC GAATCTACAA 
CAACAAATCA AGGAGCTGCA GCAGCACACA CAAGAATTAC AGCAGCAGCT GCACACACAC 
ACGGATCAGC TAAAACAGGA AGTGTCTGCG CGTGAAGAGG CAGAAAGCAG AACTCGGCTG 
TTACAGGAAG AGCTGGAGAC GACAAGGAGA GAGCGAGAGA CACTGAGAAT AGAGCGAGCA 
CTGGAGCAGA CGCAGTTCGA GGCGCAGAAG TCTCAGATGG AGGCTGAGTT TCGTCTGTCA 
TTGGAGCAGC AGATCAGTGA AAGAGTGAAG GCTGTGCAGG AGGAGAACAA CACATACAAC 
ACACAACTAC GACAGCAGCA CAGGAAGCAG CTGCTGGATC TGAGTGCGCG GCAGGAGCGA 
GAGCTGGCGG CTCAGCTGGA TCAGTGCAGA ACACAGTTAC AGGAGAGAGA CGACAAACTG 
CAGCATCTCA CACAGGCTTA CCAGCACAGA CTGTCGGAGA TGCAGGAGCA GCTGGTTTCC 
ATGGCAGCGA CTAAAAAGAA GCTAGAATCG CAGAGGGAGG AGCTTGTGTC TCGTCTTCAG 
GGAATGATGC GCTCTCATTG GGCGGAGGCT TTACGGCTGC TGACCAATCA GGAGCAGATG 
GAGAGTTTTT TATCACCCGT CCATCAGAGG GAAACATCCA GAATGTCTTC TTCTCCTAAG 
AGTGACCCAC ATGTATCAGC AGCTCCGCAG GCTGTGGTTC TGCATCTTTC CAGAGAGAAG 
GAGCAGCAGT GGGGAACACA CCGAGAGCTG AACTCAGAGA TCAACTGCAG CAACACATTC 
AGCCCACTGG AGCCACAGCT GGACAACACT GAGCTCACAG CACTCAGTGA CTGCAGTGCA 
TTGTGGGTCA GGCCGATGTT CACAATAGAT GAATCCATGA AAGAGCAGAG TGAGGAGAGG 
AGAGAAACTC AGTCAAACCT GAAGCACAAT CTGAATTACA GTCAGTGTGA ACCCAAGCTG 
AAACACAGCC ACACAGACGC ATGGACCAAT CACAACTCAG CTGCAGATAA TGTGCAGGGC 
AGGGGCGGAG CTTCTGTACT GAAACAGAGC CACGCCCCCT CAGACACGTG GACCAATAAC 
AGCTCAGCTG CAGATAGTGG GCTGGGCAGG GGCGGAGCTT CTGTACTAAA ACAGAGCCAC 
GCCCCCTTAG ACACATTGAC TATTCACAGC TCAACTGCAG ATAGTGGGCT GGCCAGGGGC 
GGAGCTTCTG TACTGAAGCA GAGCCACACC CCTTCAGACC AATGGGCCAA TCACAGCTCA 
ACTGCAGATA GTGGGCTGGG CAGGGGCGGT GTTTCTGTAC TGAAGCAGAG CAACGCCCCC 
TCAGACACAT GGACCAATCA CTGCTCAGCT GCAGATAGTG GGCTCGGCAG GGGCGTTGTT 
TCTGTACTGA AAAAGAGCCA CGCCCCCTCA GAGACATGGA CTAATAATGG CTCAGCTCCA 
GAAAGTGGGC AGAGCAGGGG TGGAGTTTCT GTACTGAAAC AAAGCCATGC CCTCTCAGAC 
ACATGGACCA ATCACAGCTC AACTGCAGAT GGTGGCAGAG GTTGGGCCTA CAGTAGTGGT 
TCTGAGAAGG CTCCGCCCCT TTCAGATGAA GCTCCACCCA GCAGGATCAG AGTTTCTGAA 
GACAGACAGA GTGAGCTGCA GTATTATGTG TCAAAGCTGC TGGAACGTTC CCCTGGTGAG 
CCGCTGGATG AGCCAATCAG AGAGCAGCGC AGTGAGTCAG CTGATGCGTC TGTCCAGCGT 
CACATGACTG ACCTGACATC AAACATGACA AACAAGAAAC CAGAGCAACA CGACAAACAA 
GAGGTGTGTG TTTCGTCTCA GCGAGGTCGA TCTGTGCAGA GCAGAAGATC TGTGAACCGC 
AGAGGAGGAT CTCAGAGAGT CTGGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325729.1
CDS	110..3139
Translation	MSVSRAAADGVSDGGLVRSWPSSPLSATRQVTARLYSSLQHSRQQEVKGHAADRQVSFALSSPDLTAVRTTSATPPLLSDRLEELSLDSASSHDVMGIGEEGVESDAEEVGLQLLKSLRSAQPTSGRKHIEEMESVRTHLQSILRPTQTTADRHDFLTPASQHFLDDSHESDATSRLLSGVCVGGVEELFPRYSRLHSDVSCAVSELQVLRDSLERERERRKVCEQQVASLQSKVLQFQQQLTLAVAADRKKDIMIEQLDKTLVKVVEGWKRHDQERNEEMKRLQEEKETAEKTHNKHAQALSRVEQNLSEVEETLKKEQKHNQELQKTNKRLEQEASDLRVHVEDLQQDKQKLSREADKHREQLHKLQTESHDTRTNLQQQIKELQQHTQELQQQLHTHTDQLKQEVSAREEAESRTRLLQEELETTRRERETLRIERALEQTQFEAQKSQMEAEFRLSLEQQISERVKAVQEENNTYNTQLRQQHRKQLLDLSARQERELAAQLDQCRTQLQERDDKLQHLTQAYQHRLSEMQEQLVSMAATKKKLESQREELVSRLQGMMRSHWAEALRLLTNQEQMESFLSPVHQRETSRMSSSPKSDPHVSAAPQAVVLHLSREKEQQWGTHRELNSEINCSNTFSPLEPQLDNTELTALSDCSALWVRPMFTIDESMKEQSEERRETQSNLKHNLNYSQCEPKLKHSHTDAWTNHNSAADNVQGRGGASVLKQSHAPSDTWTNNSSAADSGLGRGGASVLKQSHAPLDTLTIHSSTADSGLARGGASVLKQSHTPSDQWANHSSTADSGLGRGGVSVLKQSNAPSDTWTNHCSAADSGLGRGVVSVLKKSHAPSETWTNNGSAPESGQSRGGVSVLKQSHALSDTWTNHSSTADGGRGWAYSSGSEKAPPLSDEAPPSRIRVSEDRQSELQYYVSKLLERSPGEPLDEPIREQRSESADASVQRHMTDLTSNMTNKKPEQHDKQEVCVSSQRGRSVQSRRSVNRRGGSQRVWR

Target ORF information:

RefSeq Version	XM_021470054.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio centrobin, centriole duplication and spindle assembly protein (cntrob), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470054.1

ORF Insert Sequence:

ATGTCTGTGA GCCGAGCTGC TGCTGATGGT GTTAGTGATG GTGGTCTGGT GCGCTCATGG 
CCCTCGTCTC CTCTGTCTGC GACTCGTCAG GTCACCGCTC GCCTCTACAG CTCATTACAG 
CACAGCCGAC AGCAGGAGGT CAAAGGTCAC GCTGCAGACA GGCAGGTGTC ATTCGCTCTG 
TCCTCTCCTG ATCTCACCGC TGTCAGGACG ACCTCAGCCA CGCCTCCTCT GCTCTCTGAT 
AGGCTGGAGG AGCTCAGTTT GGACTCCGCC TCCTCTCATG ATGTGATGGG CATAGGAGAG 
GAGGGGGTGG AGTCAGATGC AGAAGAGGTG GGGCTTCAAC TTTTGAAGAG CCTGAGATCT 
GCACAACCGA CGAGTGGCAG GAAGCACATA GAGGAGATGG AAAGTGTGAG GACACACTTG 
CAGTCTATAC TGAGGCCAAC ACAGACAACT GCAGACAGAC ACGATTTTCT GACTCCTGCA 
TCACAGCACT TCCTGGATGA TTCACATGAG AGTGATGCCA CGTCACGCCT GCTCAGTGGT 
GTGTGTGTAG GTGGTGTGGA GGAGTTGTTC CCTCGTTATT CCCGTCTTCA TTCAGATGTG 
AGCTGCGCAG TTTCTGAGCT GCAGGTGCTG AGAGACAGTC TGGAGAGAGA GCGAGAGCGC 
CGCAAGGTGT GTGAGCAGCA GGTGGCGTCT CTCCAAAGTA AAGTTCTTCA GTTTCAGCAG 
CAGTTAACAC TCGCTGTAGC CGCCGACCGC AAGAAAGACA TCATGATTGA ACAGTTGGAC 
AAGACGCTGG TGAAGGTGGT TGAGGGCTGG AAGAGGCACG ATCAGGAGCG AAATGAGGAG 
ATGAAACGGC TGCAGGAGGA GAAGGAGACC GCTGAGAAAA CACACAACAA ACATGCACAG 
GCTCTGTCTC GTGTTGAGCA GAATCTCTCT GAGGTTGAGG AAACTCTGAA GAAGGAGCAG 
AAACACAATC AGGAGCTGCA GAAGACCAAC AAACGACTGG AGCAAGAGGC GTCTGATCTG 
CGTGTGCATG TGGAGGACTT ACAGCAAGAC AAGCAAAAGC TTAGCAGAGA AGCAGATAAA 
CACAGAGAGC AGCTCCACAA ACTCCAGACA GAGTCGCATG ACACACGGAC GAATCTACAA 
CAACAAATCA AGGAGCTGCA GCAGCACACA CAAGAATTAC AGCAGCAGCT GCACACACAC 
ACGGATCAGC TAAAACAGGA AGTGTCTGCG CGTGAAGAGG CAGAAAGCAG AACTCGGCTG 
TTACAGGAAG AGCTGGAGAC GACAAGGAGA GAGCGAGAGA CACTGAGAAT AGAGCGAGCA 
CTGGAGCAGA CGCAGTTCGA GGCGCAGAAG TCTCAGATGG AGGCTGAGTT TCGTCTGTCA 
TTGGAGCAGC AGATCAGTGA AAGAGTGAAG GCTGTGCAGG AGGAGAACAA CACATACAAC 
ACACAACTAC GACAGCAGCA CAGGAAGCAG CTGCTGGATC TGAGTGCGCG GCAGGAGCGA 
GAGCTGGCGG CTCAGCTGGA TCAGTGCAGA ACACAGTTAC AGGAGAGAGA CGACAAACTG 
CAGCATCTCA CACAGGCTTA CCAGCACAGA CTGTCGGAGA TGCAGGAGCA GCTGGTTTCC 
ATGGCAGCGA CTAAAAAGAA GCTAGAATCG CAGAGGGAGG AGCTTGTGTC TCGTCTTCAG 
GGAATGATGC GCTCTCATTG GGCGGAGGCT TTACGGCTGC TGACCAATCA GGAGCAGATG 
GAGAGTTTTT TATCACCCGT CCATCAGAGG GAAACATCCA GAATGTCTTC TTCTCCTAAG 
AGTGACCCAC ATGTATCAGC AGCTCCGCAG GCTGTGGTTC TGCATCTTTC CAGAGAGAAG 
GAGCAGCAGT GGGGAACACA CCGAGAGCTG AACTCAGAGA TCAACTGCAG CAACACATTC 
AGCCCACTGG AGCCACAGCT GGACAACACT GAGCTCACAG CACTCAGTGA CTGCAGTGCA 
TTGTGGGTCA GGCCGATGTT CACAATAGAT GAATCCATGA AAGAGCAGAG TGAGGAGAGG 
AGAGAAACTC AGTCAAACCT GAAGCACAAT CTGAATTACA GTCAGTGTGA ACCCAAGCTG 
AAACACAGCC ACACAGACGC ATGGACCAAT CACAACTCAG CTGCAGATAA TGTGCAGGGC 
AGGGGCGGAG CTTCTGTACT GAAACAGAGC CACGCCCCCT CAGACACGTG GACCAATAAC 
AGCTCAGCTG CAGATAGTGG GCTGGGCAGG GGCGGAGCTT CTGTACTAAA ACAGAGCCAC 
GCCCCCTTAG ACACATTGAC TATTCACAGC TCAACTGCAG ATAGTGGGCT GGCCAGGGGC 
GGAGCTTCTG TACTGAAGCA GAGCCACACC CCTTCAGACC AATGGGCCAA TCACAGCTCA 
ACTGCAGATA GTGGGCTGGG CAGGGGCGGT GTTTCTGTAC TGAAGCAGAG CAACGCCCCC 
TCAGACACAT GGACCAATCA CTGCTCAGCT GCAGATAGTG GGCTCGGCAG GGGCGTTGTT 
TCTGTACTGA AAAAGAGCCA CGCCCCCTCA GAGACATGGA CTAATAATGG CTCAGCTCCA 
GAAAGTGGGC AGAGCAGGGG TGGAGTTTCT GTACTGAAAC AAAGCCATGC CCTCTCAGAC 
ACATGGACCA ATCACAGCTC AACTGCAGAT GGTGGCAGAG GTTGGGCCTA CAGTAGTGGT 
TCTGAGAAGG CTCCGCCCCT TTCAGATGAA GCTCCACCCA GCAGGATCAG AGTTTCTGAA 
GACAGACAGA GTGAGCTGCA GTATTATGTG TCAAAGCTGC TGGAACGTTC CCCTGGTGAG 
CCGCTGGATG AGCCAATCAG AGAGCAGCGC AGTGAGTCAG CTGATGCGTC TGTCCAGCGT 
CACATGACTG ACCTGACATC AAACATGACA AACAAGAAAC CAGAGCAACA CGACAAACAA 
GAGGTGTGTG TTTCGTCTCA GCGAGGTCGA TCTGTGCAGA GCAGAAGATC TGTGAACCGC 
AGAGGAGGAT CTCAGAGAGT CTGGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cntrob ( XM_021470055.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325730.1 Danio rerio centrobin, centriole duplication and spindle assembly protein (cntrob), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa151621
Clone ID Related Accession (Same CDS sequence)	XM_021470055.1
Accession Version	XM_021470055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrobin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) and transcript sequence (GFIL01004366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1108 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCTGTGA GCCGAGCTGC TGCTGATGGT GTTAGTGATG GTGGTCTGGT GCGCTCATGG 
CCCTCGTCTC CTCTGTCTGC GACTCGTCAG GTCACCGCTC GCCTCTACAG CTCATTACAG 
CACAGCCGAC AGCAGGAGGT CAAAGGTCAC GCTGCAGACA GGCAGGTGTC ATTCGCTCTG 
TCCTCTCCTG ATCTCACCGC TGTCAGGACG ACCTCAGCCA CGCCTCCTCT GCTCTCTGAT 
AGGCTGGAGG AGCTCAGTTT GGACTCCGCC TCCTCTCATG ATGTGATGGG CATAGGAGAG 
GAGGGGGTGG AGTCAGATGC AGAAGAGGTG GGGCTTCAAC TTTTGAAGAG CCTGAGATCT 
GCACAACCGA CGAGTGGCAG GAAGCACATA GAGGAGATGG AAAGTGTGAG GACACACTTG 
CAGTCTATAC TGAGGCCAAC ACAGACAACT GCAGACAGAC ACGATTTTCT GACTCCTGCA 
TCACAGCACT TCCTGGATGA TTCACATGAG AGTGATGCCA CGTCACGCCT GCTCAGTGGT 
GTGTGTGTAG GTGGTGTGGA GGAGTTGTTC CCTCGTTATT CCCGTCTTCA TTCAGATGTG 
AGCTGCGCAG TTTCTGAGCT GCAGGTGCTG AGAGACAGTC TGGAGAGAGA GCGAGAGCGC 
CGCAAGGTGT GTGAGCAGCA GGTGGCGTCT CTCCAAAGTA AAGTTCTTCA GTTTCAGCAG 
CAGTTAACAC TCGCTGTAGC CGCCGACCGC AAGAAAGACA TCATGATTGA ACAGTTGGAC 
AAGACGCTGG TGAAGGTGGT TGAGGGCTGG AAGAGGCACG ATCAGGAGCG AAATGAGGAG 
ATGAAACGGC TGCAGGAGGA GAAGGAGACC GCTGAGAAAA CACACAACAA ACATGCACAG 
GCTCTGTCTC GTGTTGAGCA GAATCTCTCT GAGGTTGAGG AAACTCTGAA GAAGGAGCAG 
AAACACAATC AGGAGCTGCA GAAGACCAAC AAACGACTGG AGCAAGAGGC GTCTGATCTG 
CGTGTGCATG TGGAGGACTT ACAGCAAGAC AAGCAAAAGC TTAGCAGAGA AGCAGATAAA 
CACAGAGAGC AGCTCCACAA ACTCCAGACA GAGTCGCATG ACACACGGAC GAATCTACAA 
CAACAAATCA AGGAGCTGCA GCAGCACACA CAAGAATTAC AGCAGCAGCT GCACACACAC 
ACGGATCAGC TAAAACAGGA AGTGTCTGCG CGTGAAGAGG CAGAAAGCAG AACTCGGCTG 
TTACAGGAAG AGCTGGAGAC GACAAGGAGA GAGCGAGAGA CACTGAGAAT AGAGCGAGCA 
CTGGAGCAGA CGCAGTTCGA GGCGCAGAAG TCTCAGATGG AGGCTGAGTT TCGTCTGTCA 
TTGGAGCAGC AGATCAGTGA AAGAGTGAAG GCTGTGCAGG AGGAGAACAA CACATACAAC 
ACACAACTAC GACAGCAGCA CAGGAAGCAG CTGCTGGATC TGAGTGCGCG GCAGGAGCGA 
GAGCTGGCGG CTCAGCTGGA TCAGTGCAGA ACACAGTTAC AGGAGAGAGA CGACAAACTG 
CAGCATCTCA CACAGGCTTA CCAGCACAGA CTGTCGGAGA TGCAGGAGCA GCTGGTTTCC 
ATGGCAGCGA CTAAAAAGAA GCTAGAATCG CAGAGGGAGG AGCTTGTGTC TCGTCTTCAG 
GGAATGATGC GCTCTCATTG GGCGGAGGCT TTACGGCTGC TGACCAATCA GGAGCAGATG 
GAGAGTTTTT TATCACCCGT CCATCAGAGG GAAACATCCA GAATGTCTTC TTCTCCTAAG 
AGTGACCCAC ATGTATCAGC TCCGCAGGCT GTGGTTCTGC ATCTTTCCAG AGAGAAGGAG 
CAGCAGTGGG GAACACACCG AGAGCTGAAC TCAGAGATCA ACTGCAGCAA CACATTCAGC 
CCACTGGAGC CACAGCTGGA CAACACTGAG CTCACAGCAC TCAGTGACTG CAGTGCATTG 
TGGGTCAGGC CGATGTTCAC AATAGATGAA TCCATGAAAG AGCAGAGTGA GGAGAGGAGA 
GAAACTCAGT CAAACCTGAA GCACAATCTG AATTACAGTC AGTGTGAACC CAAGCTGAAA 
CACAGCCACA CAGACGCATG GACCAATCAC AACTCAGCTG CAGATAATGT GCAGGGCAGG 
GGCGGAGCTT CTGTACTGAA ACAGAGCCAC GCCCCCTCAG ACACGTGGAC CAATAACAGC 
TCAGCTGCAG ATAGTGGGCT GGGCAGGGGC GGAGCTTCTG TACTAAAACA GAGCCACGCC 
CCCTTAGACA CATTGACTAT TCACAGCTCA ACTGCAGATA GTGGGCTGGC CAGGGGCGGA 
GCTTCTGTAC TGAAGCAGAG CCACACCCCT TCAGACCAAT GGGCCAATCA CAGCTCAACT 
GCAGATAGTG GGCTGGGCAG GGGCGGTGTT TCTGTACTGA AGCAGAGCAA CGCCCCCTCA 
GACACATGGA CCAATCACTG CTCAGCTGCA GATAGTGGGC TCGGCAGGGG CGTTGTTTCT 
GTACTGAAAA AGAGCCACGC CCCCTCAGAG ACATGGACTA ATAATGGCTC AGCTCCAGAA 
AGTGGGCAGA GCAGGGGTGG AGTTTCTGTA CTGAAACAAA GCCATGCCCT CTCAGACACA 
TGGACCAATC ACAGCTCAAC TGCAGATGGT GGCAGAGGTT GGGCCTACAG TAGTGGTTCT 
GAGAAGGCTC CGCCCCTTTC AGATGAAGCT CCACCCAGCA GGATCAGAGT TTCTGAAGAC 
AGACAGAGTG AGCTGCAGTA TTATGTGTCA AAGCTGCTGG AACGTTCCCC TGGTGAGCCG 
CTGGATGAGC CAATCAGAGA GCAGCGCAGT GAGTCAGCTG ATGCGTCTGT CCAGCGTCAC 
ATGACTGACC TGACATCAAA CATGACAAAC AAGAAACCAG AGCAACACGA CAAACAAGAG 
GTGTGTGTTT CGTCTCAGCG AGGTCGATCT GTGCAGAGCA GAAGATCTGT GAACCGCAGA 
GGAGGATCTC AGAGAGTCTG GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325730.1
CDS	110..3136
Translation	MSVSRAAADGVSDGGLVRSWPSSPLSATRQVTARLYSSLQHSRQQEVKGHAADRQVSFALSSPDLTAVRTTSATPPLLSDRLEELSLDSASSHDVMGIGEEGVESDAEEVGLQLLKSLRSAQPTSGRKHIEEMESVRTHLQSILRPTQTTADRHDFLTPASQHFLDDSHESDATSRLLSGVCVGGVEELFPRYSRLHSDVSCAVSELQVLRDSLERERERRKVCEQQVASLQSKVLQFQQQLTLAVAADRKKDIMIEQLDKTLVKVVEGWKRHDQERNEEMKRLQEEKETAEKTHNKHAQALSRVEQNLSEVEETLKKEQKHNQELQKTNKRLEQEASDLRVHVEDLQQDKQKLSREADKHREQLHKLQTESHDTRTNLQQQIKELQQHTQELQQQLHTHTDQLKQEVSAREEAESRTRLLQEELETTRRERETLRIERALEQTQFEAQKSQMEAEFRLSLEQQISERVKAVQEENNTYNTQLRQQHRKQLLDLSARQERELAAQLDQCRTQLQERDDKLQHLTQAYQHRLSEMQEQLVSMAATKKKLESQREELVSRLQGMMRSHWAEALRLLTNQEQMESFLSPVHQRETSRMSSSPKSDPHVSAPQAVVLHLSREKEQQWGTHRELNSEINCSNTFSPLEPQLDNTELTALSDCSALWVRPMFTIDESMKEQSEERRETQSNLKHNLNYSQCEPKLKHSHTDAWTNHNSAADNVQGRGGASVLKQSHAPSDTWTNNSSAADSGLGRGGASVLKQSHAPLDTLTIHSSTADSGLARGGASVLKQSHTPSDQWANHSSTADSGLGRGGVSVLKQSNAPSDTWTNHCSAADSGLGRGVVSVLKKSHAPSETWTNNGSAPESGQSRGGVSVLKQSHALSDTWTNHSSTADGGRGWAYSSGSEKAPPLSDEAPPSRIRVSEDRQSELQYYVSKLLERSPGEPLDEPIREQRSESADASVQRHMTDLTSNMTNKKPEQHDKQEVCVSSQRGRSVQSRRSVNRRGGSQRVWR

Target ORF information:

RefSeq Version	XM_021470055.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio centrobin, centriole duplication and spindle assembly protein (cntrob), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470055.1

ORF Insert Sequence:

ATGTCTGTGA GCCGAGCTGC TGCTGATGGT GTTAGTGATG GTGGTCTGGT GCGCTCATGG 
CCCTCGTCTC CTCTGTCTGC GACTCGTCAG GTCACCGCTC GCCTCTACAG CTCATTACAG 
CACAGCCGAC AGCAGGAGGT CAAAGGTCAC GCTGCAGACA GGCAGGTGTC ATTCGCTCTG 
TCCTCTCCTG ATCTCACCGC TGTCAGGACG ACCTCAGCCA CGCCTCCTCT GCTCTCTGAT 
AGGCTGGAGG AGCTCAGTTT GGACTCCGCC TCCTCTCATG ATGTGATGGG CATAGGAGAG 
GAGGGGGTGG AGTCAGATGC AGAAGAGGTG GGGCTTCAAC TTTTGAAGAG CCTGAGATCT 
GCACAACCGA CGAGTGGCAG GAAGCACATA GAGGAGATGG AAAGTGTGAG GACACACTTG 
CAGTCTATAC TGAGGCCAAC ACAGACAACT GCAGACAGAC ACGATTTTCT GACTCCTGCA 
TCACAGCACT TCCTGGATGA TTCACATGAG AGTGATGCCA CGTCACGCCT GCTCAGTGGT 
GTGTGTGTAG GTGGTGTGGA GGAGTTGTTC CCTCGTTATT CCCGTCTTCA TTCAGATGTG 
AGCTGCGCAG TTTCTGAGCT GCAGGTGCTG AGAGACAGTC TGGAGAGAGA GCGAGAGCGC 
CGCAAGGTGT GTGAGCAGCA GGTGGCGTCT CTCCAAAGTA AAGTTCTTCA GTTTCAGCAG 
CAGTTAACAC TCGCTGTAGC CGCCGACCGC AAGAAAGACA TCATGATTGA ACAGTTGGAC 
AAGACGCTGG TGAAGGTGGT TGAGGGCTGG AAGAGGCACG ATCAGGAGCG AAATGAGGAG 
ATGAAACGGC TGCAGGAGGA GAAGGAGACC GCTGAGAAAA CACACAACAA ACATGCACAG 
GCTCTGTCTC GTGTTGAGCA GAATCTCTCT GAGGTTGAGG AAACTCTGAA GAAGGAGCAG 
AAACACAATC AGGAGCTGCA GAAGACCAAC AAACGACTGG AGCAAGAGGC GTCTGATCTG 
CGTGTGCATG TGGAGGACTT ACAGCAAGAC AAGCAAAAGC TTAGCAGAGA AGCAGATAAA 
CACAGAGAGC AGCTCCACAA ACTCCAGACA GAGTCGCATG ACACACGGAC GAATCTACAA 
CAACAAATCA AGGAGCTGCA GCAGCACACA CAAGAATTAC AGCAGCAGCT GCACACACAC 
ACGGATCAGC TAAAACAGGA AGTGTCTGCG CGTGAAGAGG CAGAAAGCAG AACTCGGCTG 
TTACAGGAAG AGCTGGAGAC GACAAGGAGA GAGCGAGAGA CACTGAGAAT AGAGCGAGCA 
CTGGAGCAGA CGCAGTTCGA GGCGCAGAAG TCTCAGATGG AGGCTGAGTT TCGTCTGTCA 
TTGGAGCAGC AGATCAGTGA AAGAGTGAAG GCTGTGCAGG AGGAGAACAA CACATACAAC 
ACACAACTAC GACAGCAGCA CAGGAAGCAG CTGCTGGATC TGAGTGCGCG GCAGGAGCGA 
GAGCTGGCGG CTCAGCTGGA TCAGTGCAGA ACACAGTTAC AGGAGAGAGA CGACAAACTG 
CAGCATCTCA CACAGGCTTA CCAGCACAGA CTGTCGGAGA TGCAGGAGCA GCTGGTTTCC 
ATGGCAGCGA CTAAAAAGAA GCTAGAATCG CAGAGGGAGG AGCTTGTGTC TCGTCTTCAG 
GGAATGATGC GCTCTCATTG GGCGGAGGCT TTACGGCTGC TGACCAATCA GGAGCAGATG 
GAGAGTTTTT TATCACCCGT CCATCAGAGG GAAACATCCA GAATGTCTTC TTCTCCTAAG 
AGTGACCCAC ATGTATCAGC TCCGCAGGCT GTGGTTCTGC ATCTTTCCAG AGAGAAGGAG 
CAGCAGTGGG GAACACACCG AGAGCTGAAC TCAGAGATCA ACTGCAGCAA CACATTCAGC 
CCACTGGAGC CACAGCTGGA CAACACTGAG CTCACAGCAC TCAGTGACTG CAGTGCATTG 
TGGGTCAGGC CGATGTTCAC AATAGATGAA TCCATGAAAG AGCAGAGTGA GGAGAGGAGA 
GAAACTCAGT CAAACCTGAA GCACAATCTG AATTACAGTC AGTGTGAACC CAAGCTGAAA 
CACAGCCACA CAGACGCATG GACCAATCAC AACTCAGCTG CAGATAATGT GCAGGGCAGG 
GGCGGAGCTT CTGTACTGAA ACAGAGCCAC GCCCCCTCAG ACACGTGGAC CAATAACAGC 
TCAGCTGCAG ATAGTGGGCT GGGCAGGGGC GGAGCTTCTG TACTAAAACA GAGCCACGCC 
CCCTTAGACA CATTGACTAT TCACAGCTCA ACTGCAGATA GTGGGCTGGC CAGGGGCGGA 
GCTTCTGTAC TGAAGCAGAG CCACACCCCT TCAGACCAAT GGGCCAATCA CAGCTCAACT 
GCAGATAGTG GGCTGGGCAG GGGCGGTGTT TCTGTACTGA AGCAGAGCAA CGCCCCCTCA 
GACACATGGA CCAATCACTG CTCAGCTGCA GATAGTGGGC TCGGCAGGGG CGTTGTTTCT 
GTACTGAAAA AGAGCCACGC CCCCTCAGAG ACATGGACTA ATAATGGCTC AGCTCCAGAA 
AGTGGGCAGA GCAGGGGTGG AGTTTCTGTA CTGAAACAAA GCCATGCCCT CTCAGACACA 
TGGACCAATC ACAGCTCAAC TGCAGATGGT GGCAGAGGTT GGGCCTACAG TAGTGGTTCT 
GAGAAGGCTC CGCCCCTTTC AGATGAAGCT CCACCCAGCA GGATCAGAGT TTCTGAAGAC 
AGACAGAGTG AGCTGCAGTA TTATGTGTCA AAGCTGCTGG AACGTTCCCC TGGTGAGCCG 
CTGGATGAGC CAATCAGAGA GCAGCGCAGT GAGTCAGCTG ATGCGTCTGT CCAGCGTCAC 
ATGACTGACC TGACATCAAA CATGACAAAC AAGAAACCAG AGCAACACGA CAAACAAGAG 
GTGTGTGTTT CGTCTCAGCG AGGTCGATCT GTGCAGAGCA GAAGATCTGT GAACCGCAGA 
GGAGGATCTC AGAGAGTCTG GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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cntrob cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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