ntng2b cDNA ORF clone, Danio rerio(zebrafish)

The following ntng2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ntng2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa25468 XM_021468967.1
Latest version!
Danio rerio netrin g2b (ntng2b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
ODa150975 XM_021468965.1
Latest version!
Danio rerio netrin g2b (ntng2b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa150976 XM_021468966.1
Latest version!
Danio rerio netrin g2b (ntng2b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa51517 XM_009295099.3
Latest version!
Danio rerio netrin g2b (ntng2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa51519 XM_009295101.2
Latest version!
Danio rerio netrin g2b (ntng2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa51521 XM_009295103.3
Latest version!
Danio rerio netrin g2b (ntng2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa51523 XM_021468964.1
Latest version!
Danio rerio netrin g2b (ntng2b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa56957 XM_017353009.2
Latest version!
Danio rerio netrin g2b (ntng2b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa56958 XM_017353010.1
Latest version!
Danio rerio netrin g2b (ntng2b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
ODa51521 XM_009295103.2 Danio rerio netrin g2b (ntng2b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa56957 XM_017353009.1 Danio rerio netrin g2b (ntng2b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa51517 XM_009295099.2 Danio rerio netrin g2b (ntng2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa25468
    Clone ID Related Accession (Same CDS sequence) XM_021468967.1
    Accession Version XM_021468967.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product netrin-G2-like isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CAATTGTGAA
    TGTAACGGAC ACTCGAATCG CTGCAGTTAC ATCGACTTCC TGAACATAGT GACGTGCGTG
    AGCTGCAAAC ACAACACGAG GGGGCAGAAC TGCGAACACT GTCGCATGAG CTACTACAAA
    AACATGTCTG CAGAGCCTGA TGATGAGAAC GTGTGTATCG AGTGTAACTG TAATGCGTTG
    GGCTCAGTTC ATGACCGCTG CAACGGCACC GGCTTCTGTC AGTGTAAAGA AGGCACAACC
    GGAGTCAAAT GTGATGAATG TCTCCCGGGC TACTATTGGA AACAGGGCTG TTTACCGAAC
    GTGTGCGACG ACGAGTTACT CTTGTGCCAG AACGGAGGGA CGTGCGTTCA GAATCAGCGC
    TGCATCTGCA CACCAGACTT TAAGGGTGTT TTGTGCCAAC ATTCGCGCTG TGAAGAAGGC
    AAAAAGTGCA ACGGCGCCAG CGCTCTCCGT TTGAGCCTGG CACTCCTGCT CTGCCCGCTT
    CTCGCCACCC TGCTGGGCTC CACCGCCTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324642.1
    CDS58..750
    Translation

    Target ORF information:

    RefSeq Version XM_021468967.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio netrin g2b (ntng2b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468967.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CAATTGTGAA
    TGTAACGGAC ACTCGAATCG CTGCAGTTAC ATCGACTTCC TGAACATAGT GACGTGCGTG
    AGCTGCAAAC ACAACACGAG GGGGCAGAAC TGCGAACACT GTCGCATGAG CTACTACAAA
    AACATGTCTG CAGAGCCTGA TGATGAGAAC GTGTGTATCG AGTGTAACTG TAATGCGTTG
    GGCTCAGTTC ATGACCGCTG CAACGGCACC GGCTTCTGTC AGTGTAAAGA AGGCACAACC
    GGAGTCAAAT GTGATGAATG TCTCCCGGGC TACTATTGGA AACAGGGCTG TTTACCGAAC
    GTGTGCGACG ACGAGTTACT CTTGTGCCAG AACGGAGGGA CGTGCGTTCA GAATCAGCGC
    TGCATCTGCA CACCAGACTT TAAGGGTGTT TTGTGCCAAC ATTCGCGCTG TGAAGAAGGC
    AAAAAGTGCA ACGGCGCCAG CGCTCTCCGT TTGAGCCTGG CACTCCTGCT CTGCCCGCTT
    CTCGCCACCC TGCTGGGCTC CACCGCCTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150975
    Clone ID Related Accession (Same CDS sequence) XM_021468965.1
    Accession Version XM_021468965.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product filaggrin-2-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGATGCT
    CCTCCGGCAC ATGTAGATAC TGTAAACCAC GTCCCATCAG CTGAAATAGA CCCCAAGGCA
    CTTTATACCA CCCCAAAAGC CACGCCCCCT TCTCATATTT TCAGAGAGGA CACTCCCCGC
    ACGGCCACAC CTCCAGAGGA GGAGCCTGTG CACATACATA TACAGAGCAA AGCACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTCAA CCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAGTAGTG AATCCTCACG AGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAAT AGTAAACCCT
    TATGAGTCAG GGCATAACTC TTATGGGTCA GTAGTGAACC CTCACGGGTC AGTAGTGAAC
    CCTCACGGGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAATAGT GAACCCTCAC GGGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAAACCTC ACGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAACCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAG
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAACCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA TCCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AATTCTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATACTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAGT AGTGAATCCT CACGAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAATA TTCATAGTTC AGAGCAGAAA TCTCATGATC CAGTAGGGAA TCCGCATGGG
    TCAGAGCATA ACCTTCATGG GTCAGAGGTG AATCATCATG GATCAGAACA TAAATCTCAT
    GAGCCACTAG TAAACCCCCA TGGGTCAGAG CATAGTCCTC ATAGGTCAGA AGTGGATCCT
    CATGGGTCAG TAGTGAATCC TCATGGGTCA GGGCATAAAT CTCATGACCC AGTTATGAAT
    CCCCATGATC CAGTAGTAAA TCCTCATGGG TCAGAGCATA AATCTCACGA TGCAGTAGTG
    AATCATCATG GGTCAGTGGA TAAATCTCAT GAACCAGTAG TGAATGCTCA TGGGTCAGAG
    CATAAGCCTC ATGGGTCAGG TCATAAACCT CATGAGTCAG TAGTGAATCC TCATGGATTG
    GAGCATAAAT CTCATGACCT AGAAGTAAAT CCTCATGGTT CAAAGCACAA CACACATGAG
    CCAATAGTGA ATCCTCATGG GACAGAGCAT AACCCTCATG GGTCATCAGC AAATCCTCAT
    GAGTCAGATC ATAAATCTCA TGAGCCAGTA GTTAATCCAC ATGGGTCAGG GCACAACCCT
    CATGGGTCAG GGCATAACCC TCACAACCCA GTAGTGAATC CTCATGTGTC AGAGCATAAA
    TCTCATGACC CAGTAGTACA CCCTCATGGG TCAGGGCATA ACAAACATGA GCCAATAGTT
    AATCCTATTG GGTCAGGGCA TAACTCCCAT GGGTCAGTAG TGAATCCTCA TGGGTCAGAG
    CATAACCCTC ATGGGTCAGT AGTGAATCCT CATGGGTCAG AGCATAACCC TCATGGGTCA
    GTAGTGAATC CTCATGGGTC AGAGCATAAC CCTCATGGGT CAGTAGTGAA TACTCATGGC
    TCAGGGCATA ACTCTCATGG GTCAGTAGCA AATCCTCATG GCTCAGAGAA TAACCCACAT
    GGGTCAGAGC ATAACTCTCA TGGGTCAGTA GCAAATCCTC ATGGGTCAGG GCATACATTT
    CATGGGTCAA TAGCAAATCC TCATGTGTCA GCGGAAAACC CTCATGGGTC AGTAGCGAAT
    CCTCATGGGT CAGAGCATAA CTCTCATGGG TCAGTAGCGA ATCCTCACGG GTCAGAGCAT
    AACCCTCATG GGTCAGAAGC GAATCCTCAT GGGTCAGAGC ATAACTCTCA TGGATCAGTA
    GCAAATCCTC ATGGGTCAGG GCATAAGCCA CATGGGTCAG AGTATAACCC TCATGGGTCA
    GTAGCGAATC CTCTTGTGTC AGAGTATAAC ACTCATGGGT CAGTAGCGAA TCCTCATGGG
    TCAGCGCATA ACCCTCATGG GTCAGTAGCG AATCCTCATG GGTCAGGGCA TGACCCACAT
    GGGTCAGAGT ATAACCCTCA TGGGTCAGTA GAAAACCCTC AGGGGTCAGC GCCTAACTCT
    CATGGATCGG TAGCAAATCC TCATGGGTCA GTAGAAAATC CTCATGCGTC AGCGCATAAC
    TCTCATGGAT CGGTAGCAAA TCCTCATGGG TCAGCGCATA ACTCTCATGG GTCGGTAGCA
    AGTCCTCATG GGTCAGCGCA TAACTCTCAT GGGTCAGGGC ATAATCCTCA TGGGTCAGGG
    CATAATCCTC ATGAGTCAGG GCATAAAACT CATGGGCCAA GGCATAAGCC TCATGGGTCA
    GTAGTGTATC CTCATGAGCC AATGCATAAC CCTCATGGAC ACCTACATAA TGAACCAAAA
    AGTGAGTCAC ATGGGCCGAT ACCAGAGTCT GCCAGGACAA CAGACGAATC CGTGCTGCCA
    ATCTCAGCAT TGGAGTCTGA TCCAAACATC GAGATGGTTT CAATACACAT GTCACCTGAA
    GAGAAAAAGG AGGGATATAC TCATAAAGCT CTTATGAAGT TCTTATTATC TGGCGGGAAA
    CAGACTGTAC AGCTAAAAGG CATCATATAT GACGACTTTC AAGATTGTGA ATGTAACGGA
    CACTCGAATC GCTGCAGTTA CATCGACTTC CTGAACATAG TGACGTGCGT GAGCTGCAAA
    CACAACACGA GGGGGCAGAA CTGCGAACAC TGTCGCATGA GCTACTACAA AAACATGTCT
    GCAGAGCCTG ATGATGAGAA CGTGTGTATC GAGTGTAACT GTAATGCGTT GGGCTCAGTT
    CATGACCGCT GCAACGGCAC CGGCTTCTGT CAGTGTAAAG AAGGCACAAC CGGAGTCAAA
    TGTGATGAAT GTCTCCCGGG CTACTATTGG AAACAGGGCT GTTTACCGAA CGTGTGCGAC
    GACGAGTTAC TCTTGTGCCA GAACGGAGGG ACGTGCGTTC AGAATCAGCG CTGCATCTGC
    ACACCAGACT TTAAGGGTGT TTTGTGCCAA CATTCGCGCT GTGAAGAAGG CAAAAAGTGC
    AACGGCGCCA GCGCTCTCCG TTTGAGCCTG GCACTCCTGC TCTGCCCGCT TCTCGCCACC
    CTGCTGGGCT CCACCGCCTC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324640.1
    CDS62..4465
    Translation

    Target ORF information:

    RefSeq Version XM_021468965.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio netrin g2b (ntng2b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468965.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGATGCT
    CCTCCGGCAC ATGTAGATAC TGTAAACCAC GTCCCATCAG CTGAAATAGA CCCCAAGGCA
    CTTTATACCA CCCCAAAAGC CACGCCCCCT TCTCATATTT TCAGAGAGGA CACTCCCCGC
    ACGGCCACAC CTCCAGAGGA GGAGCCTGTG CACATACATA TACAGAGCAA AGCACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTCAA CCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAGTAGTG AATCCTCACG AGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAAT AGTAAACCCT
    TATGAGTCAG GGCATAACTC TTATGGGTCA GTAGTGAACC CTCACGGGTC AGTAGTGAAC
    CCTCACGGGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAATAGT GAACCCTCAC GGGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAAACCTC ACGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAACCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAG
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAACCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA TCCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AATTCTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATACTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAGT AGTGAATCCT CACGAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAATA TTCATAGTTC AGAGCAGAAA TCTCATGATC CAGTAGGGAA TCCGCATGGG
    TCAGAGCATA ACCTTCATGG GTCAGAGGTG AATCATCATG GATCAGAACA TAAATCTCAT
    GAGCCACTAG TAAACCCCCA TGGGTCAGAG CATAGTCCTC ATAGGTCAGA AGTGGATCCT
    CATGGGTCAG TAGTGAATCC TCATGGGTCA GGGCATAAAT CTCATGACCC AGTTATGAAT
    CCCCATGATC CAGTAGTAAA TCCTCATGGG TCAGAGCATA AATCTCACGA TGCAGTAGTG
    AATCATCATG GGTCAGTGGA TAAATCTCAT GAACCAGTAG TGAATGCTCA TGGGTCAGAG
    CATAAGCCTC ATGGGTCAGG TCATAAACCT CATGAGTCAG TAGTGAATCC TCATGGATTG
    GAGCATAAAT CTCATGACCT AGAAGTAAAT CCTCATGGTT CAAAGCACAA CACACATGAG
    CCAATAGTGA ATCCTCATGG GACAGAGCAT AACCCTCATG GGTCATCAGC AAATCCTCAT
    GAGTCAGATC ATAAATCTCA TGAGCCAGTA GTTAATCCAC ATGGGTCAGG GCACAACCCT
    CATGGGTCAG GGCATAACCC TCACAACCCA GTAGTGAATC CTCATGTGTC AGAGCATAAA
    TCTCATGACC CAGTAGTACA CCCTCATGGG TCAGGGCATA ACAAACATGA GCCAATAGTT
    AATCCTATTG GGTCAGGGCA TAACTCCCAT GGGTCAGTAG TGAATCCTCA TGGGTCAGAG
    CATAACCCTC ATGGGTCAGT AGTGAATCCT CATGGGTCAG AGCATAACCC TCATGGGTCA
    GTAGTGAATC CTCATGGGTC AGAGCATAAC CCTCATGGGT CAGTAGTGAA TACTCATGGC
    TCAGGGCATA ACTCTCATGG GTCAGTAGCA AATCCTCATG GCTCAGAGAA TAACCCACAT
    GGGTCAGAGC ATAACTCTCA TGGGTCAGTA GCAAATCCTC ATGGGTCAGG GCATACATTT
    CATGGGTCAA TAGCAAATCC TCATGTGTCA GCGGAAAACC CTCATGGGTC AGTAGCGAAT
    CCTCATGGGT CAGAGCATAA CTCTCATGGG TCAGTAGCGA ATCCTCACGG GTCAGAGCAT
    AACCCTCATG GGTCAGAAGC GAATCCTCAT GGGTCAGAGC ATAACTCTCA TGGATCAGTA
    GCAAATCCTC ATGGGTCAGG GCATAAGCCA CATGGGTCAG AGTATAACCC TCATGGGTCA
    GTAGCGAATC CTCTTGTGTC AGAGTATAAC ACTCATGGGT CAGTAGCGAA TCCTCATGGG
    TCAGCGCATA ACCCTCATGG GTCAGTAGCG AATCCTCATG GGTCAGGGCA TGACCCACAT
    GGGTCAGAGT ATAACCCTCA TGGGTCAGTA GAAAACCCTC AGGGGTCAGC GCCTAACTCT
    CATGGATCGG TAGCAAATCC TCATGGGTCA GTAGAAAATC CTCATGCGTC AGCGCATAAC
    TCTCATGGAT CGGTAGCAAA TCCTCATGGG TCAGCGCATA ACTCTCATGG GTCGGTAGCA
    AGTCCTCATG GGTCAGCGCA TAACTCTCAT GGGTCAGGGC ATAATCCTCA TGGGTCAGGG
    CATAATCCTC ATGAGTCAGG GCATAAAACT CATGGGCCAA GGCATAAGCC TCATGGGTCA
    GTAGTGTATC CTCATGAGCC AATGCATAAC CCTCATGGAC ACCTACATAA TGAACCAAAA
    AGTGAGTCAC ATGGGCCGAT ACCAGAGTCT GCCAGGACAA CAGACGAATC CGTGCTGCCA
    ATCTCAGCAT TGGAGTCTGA TCCAAACATC GAGATGGTTT CAATACACAT GTCACCTGAA
    GAGAAAAAGG AGGGATATAC TCATAAAGCT CTTATGAAGT TCTTATTATC TGGCGGGAAA
    CAGACTGTAC AGCTAAAAGG CATCATATAT GACGACTTTC AAGATTGTGA ATGTAACGGA
    CACTCGAATC GCTGCAGTTA CATCGACTTC CTGAACATAG TGACGTGCGT GAGCTGCAAA
    CACAACACGA GGGGGCAGAA CTGCGAACAC TGTCGCATGA GCTACTACAA AAACATGTCT
    GCAGAGCCTG ATGATGAGAA CGTGTGTATC GAGTGTAACT GTAATGCGTT GGGCTCAGTT
    CATGACCGCT GCAACGGCAC CGGCTTCTGT CAGTGTAAAG AAGGCACAAC CGGAGTCAAA
    TGTGATGAAT GTCTCCCGGG CTACTATTGG AAACAGGGCT GTTTACCGAA CGTGTGCGAC
    GACGAGTTAC TCTTGTGCCA GAACGGAGGG ACGTGCGTTC AGAATCAGCG CTGCATCTGC
    ACACCAGACT TTAAGGGTGT TTTGTGCCAA CATTCGCGCT GTGAAGAAGG CAAAAAGTGC
    AACGGCGCCA GCGCTCTCCG TTTGAGCCTG GCACTCCTGC TCTGCCCGCT TCTCGCCACC
    CTGCTGGGCT CCACCGCCTC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150976
    Clone ID Related Accession (Same CDS sequence) XM_021468966.1
    Accession Version XM_021468966.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product filaggrin-2-like isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGATGCT
    CCTCCGGCAC ATGTAGATAC TGTAAACCAC GTCCCATCAG CTGAAATAGA CCCCAAGGCA
    CTTTATACCA CCCCAAAAGC CACGCCCCCT TCTCATATTT TCAGAGAGGA CACTCCCCGC
    ACGGCCACAC CTCCAGAGGA GGAGCCTGTG CACATACATA TACAGAGCAA AGCACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTCAA CCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAGTAGTG AATCCTCACG AGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAACC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA CCCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AACCCTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAACCCTCA CGAGTCAGGG
    CATAATCCTC ATGGAACAGT AGTGAATCCT CACGAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGAGTC AGGGCATAAT CCTCATGGAA CAGTAGTGAA TCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAATAGTA AACCCTCTTG ATTCAGGGCA CAACCCTCAT
    GGGTCGGTAG TGAATCCTCA TGGCTCAGGG CTTAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA TTCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AATACTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAGT AGTGAATATT CATAGTTCAG AGCAGAAATC TCATGATCCA
    GTAGGGAATC CGCATGGGTC AGAGCATAAC CTTCATGGGT CAGAGGTGAA TCATCATGGA
    TCAGAACATA AATCTCATGA GCCACTAGTA AACCCCCATG GGTCAGAGCA TAGTCCTCAT
    AGGTCAGAAG TGGATCCTCA TGGGTCAGTA GTGAATCCTC ATGGGTCAGG GCATAAATCT
    CATGACCCAG TTATGAATCC CCATGATCCA GTAGTAAATC CTCATGGGTC AGAGCATAAA
    TCTCACGATG CAGTAGTGAA TCATCATGGG TCAGTGGATA AATCTCATGA ACCAGTAGTG
    AATGCTCATG GGTCAGAGCA TAAGCCTCAT GGGTCAGGTC ATAAACCTCA TGAGTCAGTA
    GTGAATCCTC ATGGATTGGA GCATAAATCT CATGACCTAG AAGTAAATCC TCATGGTTCA
    AAGCACAACA CACATGAGCC AATAGTGAAT CCTCATGGGA CAGAGCATAA CCCTCATGGG
    TCATCAGCAA ATCCTCATGA GTCAGATCAT AAATCTCATG AGCCAGTAGT TAATCCACAT
    GGGTCAGGGC ACAACCCTCA TGGGTCAGGG CATAACCCTC ACAACCCAGT AGTGAATCCT
    CATGTGTCAG AGCATAAATC TCATGACCCA GTAGTACACC CTCATGGGTC AGGGCATAAC
    AAACATGAGC CAATAGTTAA TCCTATTGGG TCAGGGCATA ACTCCCATGG GTCAGTAGTG
    AATCCTCATG GGTCAGAGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA TGGGTCAGAG
    CATAACCCTC ATGGGTCAGT AGTGAATCCT CATGGGTCAG AGCATAACCC TCATGGGTCA
    GTAGTGAATA CTCATGGCTC AGGGCATAAC TCTCATGGGT CAGTAGCAAA TCCTCATGGC
    TCAGAGAATA ACCCACATGG GTCAGAGCAT AACTCTCATG GGTCAGTAGC AAATCCTCAT
    GGGTCAGGGC ATACATTTCA TGGGTCAATA GCAAATCCTC ATGTGTCAGC GGAAAACCCT
    CATGGGTCAG TAGCGAATCC TCATGGGTCA GAGCATAACT CTCATGGGTC AGTAGCGAAT
    CCTCACGGGT CAGAGCATAA CCCTCATGGG TCAGAAGCGA ATCCTCATGG GTCAGAGCAT
    AACTCTCATG GATCAGTAGC AAATCCTCAT GGGTCAGGGC ATAAGCCACA TGGGTCAGAG
    TATAACCCTC ATGGGTCAGT AGCGAATCCT CTTGTGTCAG AGTATAACAC TCATGGGTCA
    GTAGCGAATC CTCATGGGTC AGCGCATAAC CCTCATGGGT CAGTAGCGAA TCCTCATGGG
    TCAGGGCATG ACCCACATGG GTCAGAGTAT AACCCTCATG GGTCAGTAGA AAACCCTCAG
    GGGTCAGCGC CTAACTCTCA TGGATCGGTA GCAAATCCTC ATGGGTCAGT AGAAAATCCT
    CATGCGTCAG CGCATAACTC TCATGGATCG GTAGCAAATC CTCATGGGTC AGCGCATAAC
    TCTCATGGGT CGGTAGCAAG TCCTCATGGG TCAGCGCATA ACTCTCATGG GTCAGGGCAT
    AATCCTCATG GGTCAGGGCA TAATCCTCAT GAGTCAGGGC ATAAAACTCA TGGGCCAAGG
    CATAAGCCTC ATGGGTCAGT AGTGTATCCT CATGAGCCAA TGCATAACCC TCATGGACAC
    CTACATAATG AACCAAAAAG TGAGTCACAT GGGCCGATAC CAGAGTCTGC CAGGACAACA
    GACGAATCCG TGCTGCCAAT CTCAGCATTG GAGTCTGATC CAAACATCGA GATGGTTTCA
    ATACACATGT CACCTGAAGA GAAAAAGGAG GGATATACTC ATAAAGCTCT TATGAAGTTC
    TTATTATCTG GCGGGAAACA GACTGTACAG CTAAAAGGCA TCATATATGA CGACTTTCAA
    GATTGTGAAT GTAACGGACA CTCGAATCGC TGCAGTTACA TCGACTTCCT GAACATAGTG
    ACGTGCGTGA GCTGCAAACA CAACACGAGG GGGCAGAACT GCGAACACTG TCGCATGAGC
    TACTACAAAA ACATGTCTGC AGAGCCTGAT GATGAGAACG TGTGTATCGA GTGTAACTGT
    AATGCGTTGG GCTCAGTTCA TGACCGCTGC AACGGCACCG GCTTCTGTCA GTGTAAAGAA
    GGCACAACCG GAGTCAAATG TGATGAATGT CTCCCGGGCT ACTATTGGAA ACAGGGCTGT
    TTACCGAACG TGTGCGACGA CGAGTTACTC TTGTGCCAGA ACGGAGGGAC GTGCGTTCAG
    AATCAGCGCT GCATCTGCAC ACCAGACTTT AAGGGTGTTT TGTGCCAACA TTCGCGCTGT
    GAAGAAGGCA AAAAGTGCAA CGGCGCCAGC GCTCTCCGTT TGAGCCTGGC ACTCCTGCTC
    TGCCCGCTTC TCGCCACCCT GCTGGGCTCC ACCGCCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324641.1
    CDS62..4423
    Translation

    Target ORF information:

    RefSeq Version XM_021468966.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio netrin g2b (ntng2b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468966.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGATGCT
    CCTCCGGCAC ATGTAGATAC TGTAAACCAC GTCCCATCAG CTGAAATAGA CCCCAAGGCA
    CTTTATACCA CCCCAAAAGC CACGCCCCCT TCTCATATTT TCAGAGAGGA CACTCCCCGC
    ACGGCCACAC CTCCAGAGGA GGAGCCTGTG CACATACATA TACAGAGCAA AGCACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTCAA CCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAGTAGTG AATCCTCACG AGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAACC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA CCCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AACCCTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAACCCTCA CGAGTCAGGG
    CATAATCCTC ATGGAACAGT AGTGAATCCT CACGAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGAGTC AGGGCATAAT CCTCATGGAA CAGTAGTGAA TCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAATAGTA AACCCTCTTG ATTCAGGGCA CAACCCTCAT
    GGGTCGGTAG TGAATCCTCA TGGCTCAGGG CTTAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA TTCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AATACTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAGT AGTGAATATT CATAGTTCAG AGCAGAAATC TCATGATCCA
    GTAGGGAATC CGCATGGGTC AGAGCATAAC CTTCATGGGT CAGAGGTGAA TCATCATGGA
    TCAGAACATA AATCTCATGA GCCACTAGTA AACCCCCATG GGTCAGAGCA TAGTCCTCAT
    AGGTCAGAAG TGGATCCTCA TGGGTCAGTA GTGAATCCTC ATGGGTCAGG GCATAAATCT
    CATGACCCAG TTATGAATCC CCATGATCCA GTAGTAAATC CTCATGGGTC AGAGCATAAA
    TCTCACGATG CAGTAGTGAA TCATCATGGG TCAGTGGATA AATCTCATGA ACCAGTAGTG
    AATGCTCATG GGTCAGAGCA TAAGCCTCAT GGGTCAGGTC ATAAACCTCA TGAGTCAGTA
    GTGAATCCTC ATGGATTGGA GCATAAATCT CATGACCTAG AAGTAAATCC TCATGGTTCA
    AAGCACAACA CACATGAGCC AATAGTGAAT CCTCATGGGA CAGAGCATAA CCCTCATGGG
    TCATCAGCAA ATCCTCATGA GTCAGATCAT AAATCTCATG AGCCAGTAGT TAATCCACAT
    GGGTCAGGGC ACAACCCTCA TGGGTCAGGG CATAACCCTC ACAACCCAGT AGTGAATCCT
    CATGTGTCAG AGCATAAATC TCATGACCCA GTAGTACACC CTCATGGGTC AGGGCATAAC
    AAACATGAGC CAATAGTTAA TCCTATTGGG TCAGGGCATA ACTCCCATGG GTCAGTAGTG
    AATCCTCATG GGTCAGAGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA TGGGTCAGAG
    CATAACCCTC ATGGGTCAGT AGTGAATCCT CATGGGTCAG AGCATAACCC TCATGGGTCA
    GTAGTGAATA CTCATGGCTC AGGGCATAAC TCTCATGGGT CAGTAGCAAA TCCTCATGGC
    TCAGAGAATA ACCCACATGG GTCAGAGCAT AACTCTCATG GGTCAGTAGC AAATCCTCAT
    GGGTCAGGGC ATACATTTCA TGGGTCAATA GCAAATCCTC ATGTGTCAGC GGAAAACCCT
    CATGGGTCAG TAGCGAATCC TCATGGGTCA GAGCATAACT CTCATGGGTC AGTAGCGAAT
    CCTCACGGGT CAGAGCATAA CCCTCATGGG TCAGAAGCGA ATCCTCATGG GTCAGAGCAT
    AACTCTCATG GATCAGTAGC AAATCCTCAT GGGTCAGGGC ATAAGCCACA TGGGTCAGAG
    TATAACCCTC ATGGGTCAGT AGCGAATCCT CTTGTGTCAG AGTATAACAC TCATGGGTCA
    GTAGCGAATC CTCATGGGTC AGCGCATAAC CCTCATGGGT CAGTAGCGAA TCCTCATGGG
    TCAGGGCATG ACCCACATGG GTCAGAGTAT AACCCTCATG GGTCAGTAGA AAACCCTCAG
    GGGTCAGCGC CTAACTCTCA TGGATCGGTA GCAAATCCTC ATGGGTCAGT AGAAAATCCT
    CATGCGTCAG CGCATAACTC TCATGGATCG GTAGCAAATC CTCATGGGTC AGCGCATAAC
    TCTCATGGGT CGGTAGCAAG TCCTCATGGG TCAGCGCATA ACTCTCATGG GTCAGGGCAT
    AATCCTCATG GGTCAGGGCA TAATCCTCAT GAGTCAGGGC ATAAAACTCA TGGGCCAAGG
    CATAAGCCTC ATGGGTCAGT AGTGTATCCT CATGAGCCAA TGCATAACCC TCATGGACAC
    CTACATAATG AACCAAAAAG TGAGTCACAT GGGCCGATAC CAGAGTCTGC CAGGACAACA
    GACGAATCCG TGCTGCCAAT CTCAGCATTG GAGTCTGATC CAAACATCGA GATGGTTTCA
    ATACACATGT CACCTGAAGA GAAAAAGGAG GGATATACTC ATAAAGCTCT TATGAAGTTC
    TTATTATCTG GCGGGAAACA GACTGTACAG CTAAAAGGCA TCATATATGA CGACTTTCAA
    GATTGTGAAT GTAACGGACA CTCGAATCGC TGCAGTTACA TCGACTTCCT GAACATAGTG
    ACGTGCGTGA GCTGCAAACA CAACACGAGG GGGCAGAACT GCGAACACTG TCGCATGAGC
    TACTACAAAA ACATGTCTGC AGAGCCTGAT GATGAGAACG TGTGTATCGA GTGTAACTGT
    AATGCGTTGG GCTCAGTTCA TGACCGCTGC AACGGCACCG GCTTCTGTCA GTGTAAAGAA
    GGCACAACCG GAGTCAAATG TGATGAATGT CTCCCGGGCT ACTATTGGAA ACAGGGCTGT
    TTACCGAACG TGTGCGACGA CGAGTTACTC TTGTGCCAGA ACGGAGGGAC GTGCGTTCAG
    AATCAGCGCT GCATCTGCAC ACCAGACTTT AAGGGTGTTT TGTGCCAACA TTCGCGCTGT
    GAAGAAGGCA AAAAGTGCAA CGGCGCCAGC GCTCTCCGTT TGAGCCTGGC ACTCCTGCTC
    TGCCCGCTTC TCGCCACCCT GCTGGGCTCC ACCGCCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa51517
    Clone ID Related Accession (Same CDS sequence) XM_009295099.2 , XM_009295099.3
    Accession Version XM_009295099.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product filaggrin-2-like isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGATGCT
    CCTCCGGCAC ATGTAGATAC TGTAAACCAC GTCCCATCAG CTGAAATAGA CCCCAAGGCA
    CTTTATACCA CCCCAAAAGC CACGCCCCCT TCTCATATTT TCAGAGAGGA CACTCCCCGC
    ACGGCCACAC CTCCAGAGGA GGAGCCTGTG CACATACATA TACAGAGCAA AGCACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTCAA CCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAGTAGTG AATCCTCACG AGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAAT AGTAAACCCT
    TATGAGTCAG GGCATAACTC TTATGGGTCA GTAGTGAACC CTCACGGGTC AGTAGTGAAC
    CCTCACGGGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAATAGT GAACCCTCAC GGGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAAACCTC ACGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAACCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAG
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAACCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAACC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA CCCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AACCCTCACG AGTCAGGGCA TAATCCTCAT GGAACAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAATCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAT
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATT CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA TACTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATATTCA TAGTTCAGAG
    CAGAAATCTC ATGATCCAGT AGGGAATCCG CATGGGTCAG AGCATAACCT TCATGGGTCA
    GAGGTGAATC ATCATGGATC AGAACATAAA TCTCATGAGC CACTAGTAAA CCCCCATGGG
    TCAGAGCATA GTCCTCATAG GTCAGAAGTG GATCCTCATG GGTCAGTAGT GAATCCTCAT
    GGGTCAGGGC ATAAATCTCA TGACCCAGTT ATGAATCCCC ATGATCCAGT AGTAAATCCT
    CATGGGTCAG AGCATAAATC TCACGATGCA GTAGTGAATC ATCATGGGTC AGTGGATAAA
    TCTCATGAAC CAGTAGTGAA TGCTCATGGG TCAGAGCATA AGCCTCATGG GTCAGGTCAT
    AAACCTCATG AGTCAGTAGT GAATCCTCAT GGATTGGAGC ATAAATCTCA TGACCTAGAA
    GTAAATCCTC ATGGTTCAAA GCACAACACA CATGAGCCAA TAGTGAATCC TCATGGGACA
    GAGCATAACC CTCATGGGTC ATCAGCAAAT CCTCATGAGT CAGATCATAA ATCTCATGAG
    CCAGTAGTTA ATCCACATGG GTCAGGGCAC AACCCTCATG GGTCAGGGCA TAACCCTCAC
    AACCCAGTAG TGAATCCTCA TGTGTCAGAG CATAAATCTC ATGACCCAGT AGTACACCCT
    CATGGGTCAG GGCATAACAA ACATGAGCCA ATAGTTAATC CTATTGGGTC AGGGCATAAC
    TCCCATGGGT CAGTAGTGAA TCCTCATGGG TCAGAGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCATG GGTCAGAGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA TGGGTCAGAG
    CATAACCCTC ATGGGTCAGT AGTGAATACT CATGGCTCAG GGCATAACTC TCATGGGTCA
    GTAGCAAATC CTCATGGCTC AGAGAATAAC CCACATGGGT CAGAGCATAA CTCTCATGGG
    TCAGTAGCAA ATCCTCATGG GTCAGGGCAT ACATTTCATG GGTCAATAGC AAATCCTCAT
    GTGTCAGCGG AAAACCCTCA TGGGTCAGTA GCGAATCCTC ATGGGTCAGA GCATAACTCT
    CATGGGTCAG TAGCGAATCC TCACGGGTCA GAGCATAACC CTCATGGGTC AGAAGCGAAT
    CCTCATGGGT CAGAGCATAA CTCTCATGGA TCAGTAGCAA ATCCTCATGG GTCAGGGCAT
    AAGCCACATG GGTCAGAGTA TAACCCTCAT GGGTCAGTAG CGAATCCTCT TGTGTCAGAG
    TATAACACTC ATGGGTCAGT AGCGAATCCT CATGGGTCAG CGCATAACCC TCATGGGTCA
    GTAGCGAATC CTCATGGGTC AGGGCATGAC CCACATGGGT CAGAGTATAA CCCTCATGGG
    TCAGTAGAAA ACCCTCAGGG GTCAGCGCCT AACTCTCATG GATCGGTAGC AAATCCTCAT
    GGGTCAGTAG AAAATCCTCA TGCGTCAGCG CATAACTCTC ATGGATCGGT AGCAAATCCT
    CATGGGTCAG CGCATAACTC TCATGGGTCG GTAGCAAGTC CTCATGGGTC AGCGCATAAC
    TCTCATGGGT CAGGGCATAA TCCTCATGGG TCAGGGCATA ATCCTCATGA GTCAGGGCAT
    AAAACTCATG GGCCAAGGCA TAAGCCTCAT GGGTCAGTAG TGTATCCTCA TGAGCCAATG
    CATAACCCTC ATGGACACCT ACATAATGAA CCAAAAAGTG AGTCACATGG GCCGATACCA
    GAGTCTGCCA GGACAACAGA CGAATCCGTG CTGCCAATCT CAGCATTGGA GTCTGATCCA
    AACATCGAGA TGGTTTCAAT ACACATGTCA CCTGAAGAGA AAAAGGAGGG ATATACTCAT
    AAAGCTCTTA TGAAGTTCTT ATTATCTGGC GGGAAACAGA CTGTACAGCT AAAAGGCATC
    ATATATGACG ACTTTCAAGA TTGTGAATGT AACGGACACT CGAATCGCTG CAGTTACATC
    GACTTCCTGA ACATAGTGAC GTGCGTGAGC TGCAAACACA ACACGAGGGG GCAGAACTGC
    GAACACTGTC GCATGAGCTA CTACAAAAAC ATGTCTGCAG AGCCTGATGA TGAGAACGTG
    TGTATCGAGT GTAACTGTAA TGCGTTGGGC TCAGTTCATG ACCGCTGCAA CGGCACCGGC
    TTCTGTCAGT GTAAAGAAGG CACAACCGGA GTCAAATGTG ATGAATGTCT CCCGGGCTAC
    TATTGGAAAC AGGGCTGTTT ACCGAACGTG TGCGACGACG AGTTACTCTT GTGCCAGAAC
    GGAGGGACGT GCGTTCAGAA TCAGCGCTGC ATCTGCACAC CAGACTTTAA GGGTGTTTTG
    TGCCAACATT CGCGCTGTGA AGAAGGCAAA AAGTGCAACG GCGCCAGCGC TCTCCGTTTG
    AGCCTGGCAC TCCTGCTCTG CCCGCTTCTC GCCACCCTGC TGGGCTCCAC CGCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293374.1
    CDS60..4799
    Translation

    Target ORF information:

    RefSeq Version XM_009295099.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio netrin g2b (ntng2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295099.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGATGCT
    CCTCCGGCAC ATGTAGATAC TGTAAACCAC GTCCCATCAG CTGAAATAGA CCCCAAGGCA
    CTTTATACCA CCCCAAAAGC CACGCCCCCT TCTCATATTT TCAGAGAGGA CACTCCCCGC
    ACGGCCACAC CTCCAGAGGA GGAGCCTGTG CACATACATA TACAGAGCAA AGCACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTCAA CCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAGTAGTG AATCCTCACG AGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAAT AGTAAACCCT
    TATGAGTCAG GGCATAACTC TTATGGGTCA GTAGTGAACC CTCACGGGTC AGTAGTGAAC
    CCTCACGGGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAATAGT GAACCCTCAC GGGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAAACCTC ACGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAACCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAG
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAACCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAACC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA CCCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AACCCTCACG AGTCAGGGCA TAATCCTCAT GGAACAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAATCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAT
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATT CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA TACTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATATTCA TAGTTCAGAG
    CAGAAATCTC ATGATCCAGT AGGGAATCCG CATGGGTCAG AGCATAACCT TCATGGGTCA
    GAGGTGAATC ATCATGGATC AGAACATAAA TCTCATGAGC CACTAGTAAA CCCCCATGGG
    TCAGAGCATA GTCCTCATAG GTCAGAAGTG GATCCTCATG GGTCAGTAGT GAATCCTCAT
    GGGTCAGGGC ATAAATCTCA TGACCCAGTT ATGAATCCCC ATGATCCAGT AGTAAATCCT
    CATGGGTCAG AGCATAAATC TCACGATGCA GTAGTGAATC ATCATGGGTC AGTGGATAAA
    TCTCATGAAC CAGTAGTGAA TGCTCATGGG TCAGAGCATA AGCCTCATGG GTCAGGTCAT
    AAACCTCATG AGTCAGTAGT GAATCCTCAT GGATTGGAGC ATAAATCTCA TGACCTAGAA
    GTAAATCCTC ATGGTTCAAA GCACAACACA CATGAGCCAA TAGTGAATCC TCATGGGACA
    GAGCATAACC CTCATGGGTC ATCAGCAAAT CCTCATGAGT CAGATCATAA ATCTCATGAG
    CCAGTAGTTA ATCCACATGG GTCAGGGCAC AACCCTCATG GGTCAGGGCA TAACCCTCAC
    AACCCAGTAG TGAATCCTCA TGTGTCAGAG CATAAATCTC ATGACCCAGT AGTACACCCT
    CATGGGTCAG GGCATAACAA ACATGAGCCA ATAGTTAATC CTATTGGGTC AGGGCATAAC
    TCCCATGGGT CAGTAGTGAA TCCTCATGGG TCAGAGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCATG GGTCAGAGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA TGGGTCAGAG
    CATAACCCTC ATGGGTCAGT AGTGAATACT CATGGCTCAG GGCATAACTC TCATGGGTCA
    GTAGCAAATC CTCATGGCTC AGAGAATAAC CCACATGGGT CAGAGCATAA CTCTCATGGG
    TCAGTAGCAA ATCCTCATGG GTCAGGGCAT ACATTTCATG GGTCAATAGC AAATCCTCAT
    GTGTCAGCGG AAAACCCTCA TGGGTCAGTA GCGAATCCTC ATGGGTCAGA GCATAACTCT
    CATGGGTCAG TAGCGAATCC TCACGGGTCA GAGCATAACC CTCATGGGTC AGAAGCGAAT
    CCTCATGGGT CAGAGCATAA CTCTCATGGA TCAGTAGCAA ATCCTCATGG GTCAGGGCAT
    AAGCCACATG GGTCAGAGTA TAACCCTCAT GGGTCAGTAG CGAATCCTCT TGTGTCAGAG
    TATAACACTC ATGGGTCAGT AGCGAATCCT CATGGGTCAG CGCATAACCC TCATGGGTCA
    GTAGCGAATC CTCATGGGTC AGGGCATGAC CCACATGGGT CAGAGTATAA CCCTCATGGG
    TCAGTAGAAA ACCCTCAGGG GTCAGCGCCT AACTCTCATG GATCGGTAGC AAATCCTCAT
    GGGTCAGTAG AAAATCCTCA TGCGTCAGCG CATAACTCTC ATGGATCGGT AGCAAATCCT
    CATGGGTCAG CGCATAACTC TCATGGGTCG GTAGCAAGTC CTCATGGGTC AGCGCATAAC
    TCTCATGGGT CAGGGCATAA TCCTCATGGG TCAGGGCATA ATCCTCATGA GTCAGGGCAT
    AAAACTCATG GGCCAAGGCA TAAGCCTCAT GGGTCAGTAG TGTATCCTCA TGAGCCAATG
    CATAACCCTC ATGGACACCT ACATAATGAA CCAAAAAGTG AGTCACATGG GCCGATACCA
    GAGTCTGCCA GGACAACAGA CGAATCCGTG CTGCCAATCT CAGCATTGGA GTCTGATCCA
    AACATCGAGA TGGTTTCAAT ACACATGTCA CCTGAAGAGA AAAAGGAGGG ATATACTCAT
    AAAGCTCTTA TGAAGTTCTT ATTATCTGGC GGGAAACAGA CTGTACAGCT AAAAGGCATC
    ATATATGACG ACTTTCAAGA TTGTGAATGT AACGGACACT CGAATCGCTG CAGTTACATC
    GACTTCCTGA ACATAGTGAC GTGCGTGAGC TGCAAACACA ACACGAGGGG GCAGAACTGC
    GAACACTGTC GCATGAGCTA CTACAAAAAC ATGTCTGCAG AGCCTGATGA TGAGAACGTG
    TGTATCGAGT GTAACTGTAA TGCGTTGGGC TCAGTTCATG ACCGCTGCAA CGGCACCGGC
    TTCTGTCAGT GTAAAGAAGG CACAACCGGA GTCAAATGTG ATGAATGTCT CCCGGGCTAC
    TATTGGAAAC AGGGCTGTTT ACCGAACGTG TGCGACGACG AGTTACTCTT GTGCCAGAAC
    GGAGGGACGT GCGTTCAGAA TCAGCGCTGC ATCTGCACAC CAGACTTTAA GGGTGTTTTG
    TGCCAACATT CGCGCTGTGA AGAAGGCAAA AAGTGCAACG GCGCCAGCGC TCTCCGTTTG
    AGCCTGGCAC TCCTGCTCTG CCCGCTTCTC GCCACCCTGC TGGGCTCCAC CGCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa51517
    Clone ID Related Accession (Same CDS sequence) XM_009295099.2 , XM_009295099.3
    Accession Version XM_009295099.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product filaggrin-2-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295099.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGATGCT
    CCTCCGGCAC ATGTAGATAC TGTAAACCAC GTCCCATCAG CTGAAATAGA CCCCAAGGCA
    CTTTATACCA CCCCAAAAGC CACGCCCCCT TCTCATATTT TCAGAGAGGA CACTCCCCGC
    ACGGCCACAC CTCCAGAGGA GGAGCCTGTG CACATACATA TACAGAGCAA AGCACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTCAA CCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAGTAGTG AATCCTCACG AGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAAT AGTAAACCCT
    TATGAGTCAG GGCATAACTC TTATGGGTCA GTAGTGAACC CTCACGGGTC AGTAGTGAAC
    CCTCACGGGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAATAGT GAACCCTCAC GGGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAAACCTC ACGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAACCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAG
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAACCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAACC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA CCCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AACCCTCACG AGTCAGGGCA TAATCCTCAT GGAACAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAATCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAT
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATT CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA TACTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATATTCA TAGTTCAGAG
    CAGAAATCTC ATGATCCAGT AGGGAATCCG CATGGGTCAG AGCATAACCT TCATGGGTCA
    GAGGTGAATC ATCATGGATC AGAACATAAA TCTCATGAGC CACTAGTAAA CCCCCATGGG
    TCAGAGCATA GTCCTCATAG GTCAGAAGTG GATCCTCATG GGTCAGTAGT GAATCCTCAT
    GGGTCAGGGC ATAAATCTCA TGACCCAGTT ATGAATCCCC ATGATCCAGT AGTAAATCCT
    CATGGGTCAG AGCATAAATC TCACGATGCA GTAGTGAATC ATCATGGGTC AGTGGATAAA
    TCTCATGAAC CAGTAGTGAA TGCTCATGGG TCAGAGCATA AGCCTCATGG GTCAGGTCAT
    AAACCTCATG AGTCAGTAGT GAATCCTCAT GGATTGGAGC ATAAATCTCA TGACCTAGAA
    GTAAATCCTC ATGGTTCAAA GCACAACACA CATGAGCCAA TAGTGAATCC TCATGGGACA
    GAGCATAACC CTCATGGGTC ATCAGCAAAT CCTCATGAGT CAGATCATAA ATCTCATGAG
    CCAGTAGTTA ATCCACATGG GTCAGGGCAC AACCCTCATG GGTCAGGGCA TAACCCTCAC
    AACCCAGTAG TGAATCCTCA TGTGTCAGAG CATAAATCTC ATGACCCAGT AGTACACCCT
    CATGGGTCAG GGCATAACAA ACATGAGCCA ATAGTTAATC CTATTGGGTC AGGGCATAAC
    TCCCATGGGT CAGTAGTGAA TCCTCATGGG TCAGAGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCATG GGTCAGAGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA TGGGTCAGAG
    CATAACCCTC ATGGGTCAGT AGTGAATACT CATGGCTCAG GGCATAACTC TCATGGGTCA
    GTAGCAAATC CTCATGGCTC AGAGAATAAC CCACATGGGT CAGAGCATAA CTCTCATGGG
    TCAGTAGCAA ATCCTCATGG GTCAGGGCAT ACATTTCATG GGTCAATAGC AAATCCTCAT
    GTGTCAGCGG AAAACCCTCA TGGGTCAGTA GCGAATCCTC ATGGGTCAGA GCATAACTCT
    CATGGGTCAG TAGCGAATCC TCACGGGTCA GAGCATAACC CTCATGGGTC AGAAGCGAAT
    CCTCATGGGT CAGAGCATAA CTCTCATGGA TCAGTAGCAA ATCCTCATGG GTCAGGGCAT
    AAGCCACATG GGTCAGAGTA TAACCCTCAT GGGTCAGTAG CGAATCCTCT TGTGTCAGAG
    TATAACACTC ATGGGTCAGT AGCGAATCCT CATGGGTCAG CGCATAACCC TCATGGGTCA
    GTAGCGAATC CTCATGGGTC AGGGCATGAC CCACATGGGT CAGAGTATAA CCCTCATGGG
    TCAGTAGAAA ACCCTCAGGG GTCAGCGCCT AACTCTCATG GATCGGTAGC AAATCCTCAT
    GGGTCAGTAG AAAATCCTCA TGCGTCAGCG CATAACTCTC ATGGATCGGT AGCAAATCCT
    CATGGGTCAG CGCATAACTC TCATGGGTCG GTAGCAAGTC CTCATGGGTC AGCGCATAAC
    TCTCATGGGT CAGGGCATAA TCCTCATGGG TCAGGGCATA ATCCTCATGA GTCAGGGCAT
    AAAACTCATG GGCCAAGGCA TAAGCCTCAT GGGTCAGTAG TGTATCCTCA TGAGCCAATG
    CATAACCCTC ATGGACACCT ACATAATGAA CCAAAAAGTG AGTCACATGG GCCGATACCA
    GAGTCTGCCA GGACAACAGA CGAATCCGTG CTGCCAATCT CAGCATTGGA GTCTGATCCA
    AACATCGAGA TGGTTTCAAT ACACATGTCA CCTGAAGAGA AAAAGGAGGG ATATACTCAT
    AAAGCTCTTA TGAAGTTCTT ATTATCTGGC GGGAAACAGA CTGTACAGCT AAAAGGCATC
    ATATATGACG ACTTTCAAGA TTGTGAATGT AACGGACACT CGAATCGCTG CAGTTACATC
    GACTTCCTGA ACATAGTGAC GTGCGTGAGC TGCAAACACA ACACGAGGGG GCAGAACTGC
    GAACACTGTC GCATGAGCTA CTACAAAAAC ATGTCTGCAG AGCCTGATGA TGAGAACGTG
    TGTATCGAGT GTAACTGTAA TGCGTTGGGC TCAGTTCATG ACCGCTGCAA CGGCACCGGC
    TTCTGTCAGT GTAAAGAAGG CACAACCGGA GTCAAATGTG ATGAATGTCT CCCGGGCTAC
    TATTGGAAAC AGGGCTGTTT ACCGAACGTG TGCGACGACG AGTTACTCTT GTGCCAGAAC
    GGAGGGACGT GCGTTCAGAA TCAGCGCTGC ATCTGCACAC CAGACTTTAA GGGTGTTTTG
    TGCCAACATT CGCGCTGTGA AGAAGGCAAA AAGTGCAACG GCGCCAGCGC TCTCCGTTTG
    AGCCTGGCAC TCCTGCTCTG CCCGCTTCTC GCCACCCTGC TGGGCTCCAC CGCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293374.1
    CDS62..4801
    Translation

    Target ORF information:

    RefSeq Version XM_009295099.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio netrin g2b (ntng2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295099.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGATGCT
    CCTCCGGCAC ATGTAGATAC TGTAAACCAC GTCCCATCAG CTGAAATAGA CCCCAAGGCA
    CTTTATACCA CCCCAAAAGC CACGCCCCCT TCTCATATTT TCAGAGAGGA CACTCCCCGC
    ACGGCCACAC CTCCAGAGGA GGAGCCTGTG CACATACATA TACAGAGCAA AGCACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTCAA CCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAGTAGTG AATCCTCACG AGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAAT AGTAAACCCT
    TATGAGTCAG GGCATAACTC TTATGGGTCA GTAGTGAACC CTCACGGGTC AGTAGTGAAC
    CCTCACGGGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAATAGT GAACCCTCAC GGGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAAACCTC ACGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAACCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAG
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAACCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAACC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA CCCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AACCCTCACG AGTCAGGGCA TAATCCTCAT GGAACAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAATCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAT
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATT CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA TACTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATATTCA TAGTTCAGAG
    CAGAAATCTC ATGATCCAGT AGGGAATCCG CATGGGTCAG AGCATAACCT TCATGGGTCA
    GAGGTGAATC ATCATGGATC AGAACATAAA TCTCATGAGC CACTAGTAAA CCCCCATGGG
    TCAGAGCATA GTCCTCATAG GTCAGAAGTG GATCCTCATG GGTCAGTAGT GAATCCTCAT
    GGGTCAGGGC ATAAATCTCA TGACCCAGTT ATGAATCCCC ATGATCCAGT AGTAAATCCT
    CATGGGTCAG AGCATAAATC TCACGATGCA GTAGTGAATC ATCATGGGTC AGTGGATAAA
    TCTCATGAAC CAGTAGTGAA TGCTCATGGG TCAGAGCATA AGCCTCATGG GTCAGGTCAT
    AAACCTCATG AGTCAGTAGT GAATCCTCAT GGATTGGAGC ATAAATCTCA TGACCTAGAA
    GTAAATCCTC ATGGTTCAAA GCACAACACA CATGAGCCAA TAGTGAATCC TCATGGGACA
    GAGCATAACC CTCATGGGTC ATCAGCAAAT CCTCATGAGT CAGATCATAA ATCTCATGAG
    CCAGTAGTTA ATCCACATGG GTCAGGGCAC AACCCTCATG GGTCAGGGCA TAACCCTCAC
    AACCCAGTAG TGAATCCTCA TGTGTCAGAG CATAAATCTC ATGACCCAGT AGTACACCCT
    CATGGGTCAG GGCATAACAA ACATGAGCCA ATAGTTAATC CTATTGGGTC AGGGCATAAC
    TCCCATGGGT CAGTAGTGAA TCCTCATGGG TCAGAGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCATG GGTCAGAGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA TGGGTCAGAG
    CATAACCCTC ATGGGTCAGT AGTGAATACT CATGGCTCAG GGCATAACTC TCATGGGTCA
    GTAGCAAATC CTCATGGCTC AGAGAATAAC CCACATGGGT CAGAGCATAA CTCTCATGGG
    TCAGTAGCAA ATCCTCATGG GTCAGGGCAT ACATTTCATG GGTCAATAGC AAATCCTCAT
    GTGTCAGCGG AAAACCCTCA TGGGTCAGTA GCGAATCCTC ATGGGTCAGA GCATAACTCT
    CATGGGTCAG TAGCGAATCC TCACGGGTCA GAGCATAACC CTCATGGGTC AGAAGCGAAT
    CCTCATGGGT CAGAGCATAA CTCTCATGGA TCAGTAGCAA ATCCTCATGG GTCAGGGCAT
    AAGCCACATG GGTCAGAGTA TAACCCTCAT GGGTCAGTAG CGAATCCTCT TGTGTCAGAG
    TATAACACTC ATGGGTCAGT AGCGAATCCT CATGGGTCAG CGCATAACCC TCATGGGTCA
    GTAGCGAATC CTCATGGGTC AGGGCATGAC CCACATGGGT CAGAGTATAA CCCTCATGGG
    TCAGTAGAAA ACCCTCAGGG GTCAGCGCCT AACTCTCATG GATCGGTAGC AAATCCTCAT
    GGGTCAGTAG AAAATCCTCA TGCGTCAGCG CATAACTCTC ATGGATCGGT AGCAAATCCT
    CATGGGTCAG CGCATAACTC TCATGGGTCG GTAGCAAGTC CTCATGGGTC AGCGCATAAC
    TCTCATGGGT CAGGGCATAA TCCTCATGGG TCAGGGCATA ATCCTCATGA GTCAGGGCAT
    AAAACTCATG GGCCAAGGCA TAAGCCTCAT GGGTCAGTAG TGTATCCTCA TGAGCCAATG
    CATAACCCTC ATGGACACCT ACATAATGAA CCAAAAAGTG AGTCACATGG GCCGATACCA
    GAGTCTGCCA GGACAACAGA CGAATCCGTG CTGCCAATCT CAGCATTGGA GTCTGATCCA
    AACATCGAGA TGGTTTCAAT ACACATGTCA CCTGAAGAGA AAAAGGAGGG ATATACTCAT
    AAAGCTCTTA TGAAGTTCTT ATTATCTGGC GGGAAACAGA CTGTACAGCT AAAAGGCATC
    ATATATGACG ACTTTCAAGA TTGTGAATGT AACGGACACT CGAATCGCTG CAGTTACATC
    GACTTCCTGA ACATAGTGAC GTGCGTGAGC TGCAAACACA ACACGAGGGG GCAGAACTGC
    GAACACTGTC GCATGAGCTA CTACAAAAAC ATGTCTGCAG AGCCTGATGA TGAGAACGTG
    TGTATCGAGT GTAACTGTAA TGCGTTGGGC TCAGTTCATG ACCGCTGCAA CGGCACCGGC
    TTCTGTCAGT GTAAAGAAGG CACAACCGGA GTCAAATGTG ATGAATGTCT CCCGGGCTAC
    TATTGGAAAC AGGGCTGTTT ACCGAACGTG TGCGACGACG AGTTACTCTT GTGCCAGAAC
    GGAGGGACGT GCGTTCAGAA TCAGCGCTGC ATCTGCACAC CAGACTTTAA GGGTGTTTTG
    TGCCAACATT CGCGCTGTGA AGAAGGCAAA AAGTGCAACG GCGCCAGCGC TCTCCGTTTG
    AGCCTGGCAC TCCTGCTCTG CCCGCTTCTC GCCACCCTGC TGGGCTCCAC CGCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa51519
    Clone ID Related Accession (Same CDS sequence) XM_009295101.2
    Accession Version XM_009295101.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product filaggrin-2-like isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CAATGCTCCT
    CCGGCACATG TAGATACTGT AAACCACGTC CCATCAGCTG AAATAGACCC CAAGGCACTT
    TATACCACCC CAAAAGCCAC GCCCCCTTCT CATATTTTCA GAGAGGACAC TCCCCGCACG
    GCCACACCTC CAGAGGAGGA GCCTGTGCAC ATACATATAC AGAGCAAAGC ACCACCAGTG
    GAGGATTCTG GGGAAGTTGA AGAGATTTCT GAAAAGAAAG AGATGACTTC AGGGAGACAT
    GAAGTATCTG AAGATGTAAA GAGAGTGGAG ACTTTTCTGG AACATAACAG ACACAGGGAA
    GAAACTGCAC CTGAACCTGT TGGATCAGTG CGGGAGTCTG CCGAGCCAAA GCCAGAGTCG
    ATTCCAAAGC CAATTTTGGA AACACACAAG CCACTACACA AACCTCAAGT TCATGTGTCT
    ACAGCGGAAT CTCATGGAGC AGCACAAACA TCACATGAAT CAGTAACAAA GCATCATGAA
    CCAATTCAGA AACTTCATGA GTCAACAGTG AAACCTCACT CAATGCATAC AACCCATGAG
    ACTATACAGA AACCTCATGA ACCAAAAGTG AATTCTCATG AATCAATGCA TAAACCCCAT
    GAGCAAATTG TAAATCTTAA TGAATCAATG TATAAGCCTC ATCAGCCAAT ATGGGATCCA
    CATTCATCGC CGCAAAAACC TTTTGAGCCA AACGTGAACC CCCTTGAGTC AGTAGTGAAT
    ATTCATGAGT CAACGCATAA ATCACATGAA GCAGTAGTGA ATCCTCAGGA ATCAATGAAT
    AACCCTCATG GGTCAGTAGT AAACCCTCAC AAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAACCCTC ACGGGTCAGG GCATAACCCT CATGGGTCAG TAGTCAACCC TCACGAGTCA
    GGGCATAACC CTCATGGGTC AGTAGTGAAT CCTCACGAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAATAGT AAACCCTTAT
    GAGTCAGGGC ATAACTCTTA TGGGTCAGTA GTGAACCCTC ACGGGTCAGT AGTGAACCCT
    CACGGGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAATAGTGAA CCCTCACGGG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AAACCTCACG GGTCAGTAGT GAACCCTCAC GAGTCAGGGC ATAACCCTCA TGGAACAGTA
    GTGAATCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAA TAGTAAACCC TCTTGAGTCA
    GGGCACAACC CTCATGGGTC GGTAGTGAAT CCTCATGGCT CAGGGCTTAA CCCTCATGGG
    TCAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAGTAGT GAACCCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAACCCTC ACGAGTCAGG GCATAACCCT
    CATGGGTCAG TAGTGAACCC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAC
    CCTCACGAGT CAGGGCATAA TCCTCATGGA ACAGTAGTGA ATCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAGTAGT GAACCCTCAC GAGTCAGGGC ATAATCCTCA TGGAACAGTA
    GTGAATCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAA TAGTAAACCC TCTTGATTCA
    GGGCACAACC CTCATGGGTC GGTAGTGAAT CCTCATGGCT CAGGGCTTAA CCCTCATGGG
    TCAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAGTAGT GAATCCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAATTCTC ACGAGTCAGG GCATAACCCT
    CATGGGTCAG TAGTGAATAC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAT
    CCTCACGAGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATATTCATAG TTCAGAGCAG
    AAATCTCATG ATCCAGTAGG GAATCCGCAT GGGTCAGAGC ATAACCTTCA TGGGTCAGAG
    GTGAATCATC ATGGATCAGA ACATAAATCT CATGAGCCAC TAGTAAACCC CCATGGGTCA
    GAGCATAGTC CTCATAGGTC AGAAGTGGAT CCTCATGGGT CAGTAGTGAA TCCTCATGGG
    TCAGGGCATA AATCTCATGA CCCAGTTATG AATCCCCATG ATCCAGTAGT AAATCCTCAT
    GGGTCAGAGC ATAAATCTCA CGATGCAGTA GTGAATCATC ATGGGTCAGT GGATAAATCT
    CATGAACCAG TAGTGAATGC TCATGGGTCA GAGCATAAGC CTCATGGGTC AGGTCATAAA
    CCTCATGAGT CAGTAGTGAA TCCTCATGGA TTGGAGCATA AATCTCATGA CCTAGAAGTA
    AATCCTCATG GTTCAAAGCA CAACACACAT GAGCCAATAG TGAATCCTCA TGGGACAGAG
    CATAACCCTC ATGGGTCATC AGCAAATCCT CATGAGTCAG ATCATAAATC TCATGAGCCA
    GTAGTTAATC CACATGGGTC AGGGCACAAC CCTCATGGGT CAGGGCATAA CCCTCACAAC
    CCAGTAGTGA ATCCTCATGT GTCAGAGCAT AAATCTCATG ACCCAGTAGT ACACCCTCAT
    GGGTCAGGGC ATAACAAACA TGAGCCAATA GTTAATCCTA TTGGGTCAGG GCATAACTCC
    CATGGGTCAG TAGTGAATCC TCATGGGTCA GAGCATAACC CTCATGGGTC AGTAGTGAAT
    CCTCATGGGT CAGAGCATAA CCCTCATGGG TCAGTAGTGA ATCCTCATGG GTCAGAGCAT
    AACCCTCATG GGTCAGTAGT GAATACTCAT GGCTCAGGGC ATAACTCTCA TGGGTCAGTA
    GCAAATCCTC ATGGCTCAGA GAATAACCCA CATGGGTCAG AGCATAACTC TCATGGGTCA
    GTAGCAAATC CTCATGGGTC AGGGCATACA TTTCATGGGT CAATAGCAAA TCCTCATGTG
    TCAGCGGAAA ACCCTCATGG GTCAGTAGCG AATCCTCATG GGTCAGAGCA TAACTCTCAT
    GGGTCAGTAG CGAATCCTCA CGGGTCAGAG CATAACCCTC ATGGGTCAGA AGCGAATCCT
    CATGGGTCAG AGCATAACTC TCATGGATCA GTAGCAAATC CTCATGGGTC AGGGCATAAG
    CCACATGGGT CAGAGTATAA CCCTCATGGG TCAGTAGCGA ATCCTCTTGT GTCAGAGTAT
    AACACTCATG GGTCAGTAGC GAATCCTCAT GGGTCAGCGC ATAACCCTCA TGGGTCAGTA
    GCGAATCCTC ATGGGTCAGG GCATGACCCA CATGGGTCAG AGTATAACCC TCATGGGTCA
    GTAGAAAACC CTCAGGGGTC AGCGCCTAAC TCTCATGGAT CGGTAGCAAA TCCTCATGGG
    TCAGTAGAAA ATCCTCATGC GTCAGCGCAT AACTCTCATG GATCGGTAGC AAATCCTCAT
    GGGTCAGCGC ATAACTCTCA TGGGTCGGTA GCAAGTCCTC ATGGGTCAGC GCATAACTCT
    CATGGGTCAG GGCATAATCC TCATGGGTCA GGGCATAATC CTCATGAGTC AGGGCATAAA
    ACTCATGGGC CAAGGCATAA GCCTCATGGG TCAGTAGTGT ATCCTCATGA GCCAATGCAT
    AACCCTCATG GACACCTACA TAATGAACCA AAAAGTGAGT CACATGGGCC GATACCAGAG
    TCTGCCAGGA CAACAGACGA ATCCGTGCTG CCAATCTCAG CATTGGAGTC TGATCCAAAC
    ATCGAGATGG TTTCAATACA CATGTCACCT GAAGAGAAAA AGGAGGGATA TACTCATAAA
    GCTCTTATGA AGTTCTTATT ATCTGGCGGG AAACAGACTG TACAGCTAAA AGGCATCATA
    TATGACGACT TTCAAGATTG TGAATGTAAC GGACACTCGA ATCGCTGCAG TTACATCGAC
    TTCCTGAACA TAGTGACGTG CGTGAGCTGC AAACACAACA CGAGGGGGCA GAACTGCGAA
    CACTGTCGCA TGAGCTACTA CAAAAACATG TCTGCAGAGC CTGATGATGA GAACGTGTGT
    ATCGAGTGTA ACTGTAATGC GTTGGGCTCA GTTCATGACC GCTGCAACGG CACCGGCTTC
    TGTCAGTGTA AAGAAGGCAC AACCGGAGTC AAATGTGATG AATGTCTCCC GGGCTACTAT
    TGGAAACAGG GCTGTTTACC GAACGTGTGC GACGACGAGT TACTCTTGTG CCAGAACGGA
    GGGACGTGCG TTCAGAATCA GCGCTGCATC TGCACACCAG ACTTTAAGGG TGTTTTGTGC
    CAACATTCGC GCTGTGAAGA AGGCAAAAAG TGCAACGGCG CCAGCGCTCT CCGTTTGAGC
    CTGGCACTCC TGCTCTGCCC GCTTCTCGCC ACCCTGCTGG GCTCCACCGC CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293376.1
    CDS60..4736
    Translation

    Target ORF information:

    RefSeq Version XM_009295101.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio netrin g2b (ntng2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295101.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CAATGCTCCT
    CCGGCACATG TAGATACTGT AAACCACGTC CCATCAGCTG AAATAGACCC CAAGGCACTT
    TATACCACCC CAAAAGCCAC GCCCCCTTCT CATATTTTCA GAGAGGACAC TCCCCGCACG
    GCCACACCTC CAGAGGAGGA GCCTGTGCAC ATACATATAC AGAGCAAAGC ACCACCAGTG
    GAGGATTCTG GGGAAGTTGA AGAGATTTCT GAAAAGAAAG AGATGACTTC AGGGAGACAT
    GAAGTATCTG AAGATGTAAA GAGAGTGGAG ACTTTTCTGG AACATAACAG ACACAGGGAA
    GAAACTGCAC CTGAACCTGT TGGATCAGTG CGGGAGTCTG CCGAGCCAAA GCCAGAGTCG
    ATTCCAAAGC CAATTTTGGA AACACACAAG CCACTACACA AACCTCAAGT TCATGTGTCT
    ACAGCGGAAT CTCATGGAGC AGCACAAACA TCACATGAAT CAGTAACAAA GCATCATGAA
    CCAATTCAGA AACTTCATGA GTCAACAGTG AAACCTCACT CAATGCATAC AACCCATGAG
    ACTATACAGA AACCTCATGA ACCAAAAGTG AATTCTCATG AATCAATGCA TAAACCCCAT
    GAGCAAATTG TAAATCTTAA TGAATCAATG TATAAGCCTC ATCAGCCAAT ATGGGATCCA
    CATTCATCGC CGCAAAAACC TTTTGAGCCA AACGTGAACC CCCTTGAGTC AGTAGTGAAT
    ATTCATGAGT CAACGCATAA ATCACATGAA GCAGTAGTGA ATCCTCAGGA ATCAATGAAT
    AACCCTCATG GGTCAGTAGT AAACCCTCAC AAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAACCCTC ACGGGTCAGG GCATAACCCT CATGGGTCAG TAGTCAACCC TCACGAGTCA
    GGGCATAACC CTCATGGGTC AGTAGTGAAT CCTCACGAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAATAGT AAACCCTTAT
    GAGTCAGGGC ATAACTCTTA TGGGTCAGTA GTGAACCCTC ACGGGTCAGT AGTGAACCCT
    CACGGGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAATAGTGAA CCCTCACGGG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AAACCTCACG GGTCAGTAGT GAACCCTCAC GAGTCAGGGC ATAACCCTCA TGGAACAGTA
    GTGAATCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAA TAGTAAACCC TCTTGAGTCA
    GGGCACAACC CTCATGGGTC GGTAGTGAAT CCTCATGGCT CAGGGCTTAA CCCTCATGGG
    TCAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAGTAGT GAACCCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAACCCTC ACGAGTCAGG GCATAACCCT
    CATGGGTCAG TAGTGAACCC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAC
    CCTCACGAGT CAGGGCATAA TCCTCATGGA ACAGTAGTGA ATCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAGTAGT GAACCCTCAC GAGTCAGGGC ATAATCCTCA TGGAACAGTA
    GTGAATCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAA TAGTAAACCC TCTTGATTCA
    GGGCACAACC CTCATGGGTC GGTAGTGAAT CCTCATGGCT CAGGGCTTAA CCCTCATGGG
    TCAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAGTAGT GAATCCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAATTCTC ACGAGTCAGG GCATAACCCT
    CATGGGTCAG TAGTGAATAC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAT
    CCTCACGAGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATATTCATAG TTCAGAGCAG
    AAATCTCATG ATCCAGTAGG GAATCCGCAT GGGTCAGAGC ATAACCTTCA TGGGTCAGAG
    GTGAATCATC ATGGATCAGA ACATAAATCT CATGAGCCAC TAGTAAACCC CCATGGGTCA
    GAGCATAGTC CTCATAGGTC AGAAGTGGAT CCTCATGGGT CAGTAGTGAA TCCTCATGGG
    TCAGGGCATA AATCTCATGA CCCAGTTATG AATCCCCATG ATCCAGTAGT AAATCCTCAT
    GGGTCAGAGC ATAAATCTCA CGATGCAGTA GTGAATCATC ATGGGTCAGT GGATAAATCT
    CATGAACCAG TAGTGAATGC TCATGGGTCA GAGCATAAGC CTCATGGGTC AGGTCATAAA
    CCTCATGAGT CAGTAGTGAA TCCTCATGGA TTGGAGCATA AATCTCATGA CCTAGAAGTA
    AATCCTCATG GTTCAAAGCA CAACACACAT GAGCCAATAG TGAATCCTCA TGGGACAGAG
    CATAACCCTC ATGGGTCATC AGCAAATCCT CATGAGTCAG ATCATAAATC TCATGAGCCA
    GTAGTTAATC CACATGGGTC AGGGCACAAC CCTCATGGGT CAGGGCATAA CCCTCACAAC
    CCAGTAGTGA ATCCTCATGT GTCAGAGCAT AAATCTCATG ACCCAGTAGT ACACCCTCAT
    GGGTCAGGGC ATAACAAACA TGAGCCAATA GTTAATCCTA TTGGGTCAGG GCATAACTCC
    CATGGGTCAG TAGTGAATCC TCATGGGTCA GAGCATAACC CTCATGGGTC AGTAGTGAAT
    CCTCATGGGT CAGAGCATAA CCCTCATGGG TCAGTAGTGA ATCCTCATGG GTCAGAGCAT
    AACCCTCATG GGTCAGTAGT GAATACTCAT GGCTCAGGGC ATAACTCTCA TGGGTCAGTA
    GCAAATCCTC ATGGCTCAGA GAATAACCCA CATGGGTCAG AGCATAACTC TCATGGGTCA
    GTAGCAAATC CTCATGGGTC AGGGCATACA TTTCATGGGT CAATAGCAAA TCCTCATGTG
    TCAGCGGAAA ACCCTCATGG GTCAGTAGCG AATCCTCATG GGTCAGAGCA TAACTCTCAT
    GGGTCAGTAG CGAATCCTCA CGGGTCAGAG CATAACCCTC ATGGGTCAGA AGCGAATCCT
    CATGGGTCAG AGCATAACTC TCATGGATCA GTAGCAAATC CTCATGGGTC AGGGCATAAG
    CCACATGGGT CAGAGTATAA CCCTCATGGG TCAGTAGCGA ATCCTCTTGT GTCAGAGTAT
    AACACTCATG GGTCAGTAGC GAATCCTCAT GGGTCAGCGC ATAACCCTCA TGGGTCAGTA
    GCGAATCCTC ATGGGTCAGG GCATGACCCA CATGGGTCAG AGTATAACCC TCATGGGTCA
    GTAGAAAACC CTCAGGGGTC AGCGCCTAAC TCTCATGGAT CGGTAGCAAA TCCTCATGGG
    TCAGTAGAAA ATCCTCATGC GTCAGCGCAT AACTCTCATG GATCGGTAGC AAATCCTCAT
    GGGTCAGCGC ATAACTCTCA TGGGTCGGTA GCAAGTCCTC ATGGGTCAGC GCATAACTCT
    CATGGGTCAG GGCATAATCC TCATGGGTCA GGGCATAATC CTCATGAGTC AGGGCATAAA
    ACTCATGGGC CAAGGCATAA GCCTCATGGG TCAGTAGTGT ATCCTCATGA GCCAATGCAT
    AACCCTCATG GACACCTACA TAATGAACCA AAAAGTGAGT CACATGGGCC GATACCAGAG
    TCTGCCAGGA CAACAGACGA ATCCGTGCTG CCAATCTCAG CATTGGAGTC TGATCCAAAC
    ATCGAGATGG TTTCAATACA CATGTCACCT GAAGAGAAAA AGGAGGGATA TACTCATAAA
    GCTCTTATGA AGTTCTTATT ATCTGGCGGG AAACAGACTG TACAGCTAAA AGGCATCATA
    TATGACGACT TTCAAGATTG TGAATGTAAC GGACACTCGA ATCGCTGCAG TTACATCGAC
    TTCCTGAACA TAGTGACGTG CGTGAGCTGC AAACACAACA CGAGGGGGCA GAACTGCGAA
    CACTGTCGCA TGAGCTACTA CAAAAACATG TCTGCAGAGC CTGATGATGA GAACGTGTGT
    ATCGAGTGTA ACTGTAATGC GTTGGGCTCA GTTCATGACC GCTGCAACGG CACCGGCTTC
    TGTCAGTGTA AAGAAGGCAC AACCGGAGTC AAATGTGATG AATGTCTCCC GGGCTACTAT
    TGGAAACAGG GCTGTTTACC GAACGTGTGC GACGACGAGT TACTCTTGTG CCAGAACGGA
    GGGACGTGCG TTCAGAATCA GCGCTGCATC TGCACACCAG ACTTTAAGGG TGTTTTGTGC
    CAACATTCGC GCTGTGAAGA AGGCAAAAAG TGCAACGGCG CCAGCGCTCT CCGTTTGAGC
    CTGGCACTCC TGCTCTGCCC GCTTCTCGCC ACCCTGCTGG GCTCCACCGC CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa51521
    Clone ID Related Accession (Same CDS sequence) XM_009295103.3 , XM_009295103.2
    Accession Version XM_009295103.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product filaggrin-2-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295103.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGCACCA
    CCAGTGGAGG ATTCTGGGGA AGTTGAAGAG ATTTCTGAAA AGAAAGAGAT GACTTCAGGG
    AGACATGAAG TATCTGAAGA TGTAAAGAGA GTGGAGACTT TTCTGGAACA TAACAGACAC
    AGGGAAGAAA CTGCACCTGA ACCTGTTGGA TCAGTGCGGG AGTCTGCCGA GCCAAAGCCA
    GAGTCGATTC CAAAGCCAAT TTTGGAAACA CACAAGCCAC TACACAAACC TCAAGTTCAT
    GTGTCTACAG CGGAATCTCA TGGAGCAGCA CAAACATCAC ATGAATCAGT AACAAAGCAT
    CATGAACCAA TTCAGAAACT TCATGAGTCA ACAGTGAAAC CTCACTCAAT GCATACAACC
    CATGAGACTA TACAGAAACC TCATGAACCA AAAGTGAATT CTCATGAATC AATGCATAAA
    CCCCATGAGC AAATTGTAAA TCTTAATGAA TCAATGTATA AGCCTCATCA GCCAATATGG
    GATCCACATT CATCGCCGCA AAAACCTTTT GAGCCAAACG TGAACCCCCT TGAGTCAGTA
    GTGAATATTC ATGAGTCAAC GCATAAATCA CATGAAGCAG TAGTGAATCC TCAGGAATCA
    ATGAATAACC CTCATGGGTC AGTAGTAAAC CCTCACAAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ACCCTCACGG GTCAGGGCAT AACCCTCATG GGTCAGTAGT CAACCCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAATCCTC ACGAGTCAGG GCATAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AATAGTAAAC
    CCTTATGAGT CAGGGCATAA CTCTTATGGG TCAGTAGTGA ACCCTCACGG GTCAGTAGTG
    AACCCTCACG GGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAAT AGTGAACCCT CACGGGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAAAC CTCACGGGTC AGTAGTGAAC CCTCACGAGT CAGGGCATAA CCCTCATGGA
    ACAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAATAGT AAACCCTCTT
    GAGTCAGGGC ACAACCCTCA TGGGTCGGTA GTGAATCCTC ATGGCTCAGG GCTTAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAC
    CCTCACGAGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ACCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAGTAGT GAACCCTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAACCCTC ACGAGTCAGG GCATAATCCT CATGGAACAG TAGTGAATCC TCACGAGTCA
    GGGCATAACC CTCATGGGTC AGTAGTGAAC CCTCACGAGT CAGGGCATAA TCCTCATGGA
    ACAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAATAGT AAACCCTCTT
    GATTCAGGGC ACAACCCTCA TGGGTCGGTA GTGAATCCTC ATGGCTCAGG GCTTAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAT
    CCTCACGAGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATTCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAGTAGT GAATACTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAATCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAG TAGTGAATAT TCATAGTTCA
    GAGCAGAAAT CTCATGATCC AGTAGGGAAT CCGCATGGGT CAGAGCATAA CCTTCATGGG
    TCAGAGGTGA ATCATCATGG ATCAGAACAT AAATCTCATG AGCCACTAGT AAACCCCCAT
    GGGTCAGAGC ATAGTCCTCA TAGGTCAGAA GTGGATCCTC ATGGGTCAGT AGTGAATCCT
    CATGGGTCAG GGCATAAATC TCATGACCCA GTTATGAATC CCCATGATCC AGTAGTAAAT
    CCTCATGGGT CAGAGCATAA ATCTCACGAT GCAGTAGTGA ATCATCATGG GTCAGTGGAT
    AAATCTCATG AACCAGTAGT GAATGCTCAT GGGTCAGAGC ATAAGCCTCA TGGGTCAGGT
    CATAAACCTC ATGAGTCAGT AGTGAATCCT CATGGATTGG AGCATAAATC TCATGACCTA
    GAAGTAAATC CTCATGGTTC AAAGCACAAC ACACATGAGC CAATAGTGAA TCCTCATGGG
    ACAGAGCATA ACCCTCATGG GTCATCAGCA AATCCTCATG AGTCAGATCA TAAATCTCAT
    GAGCCAGTAG TTAATCCACA TGGGTCAGGG CACAACCCTC ATGGGTCAGG GCATAACCCT
    CACAACCCAG TAGTGAATCC TCATGTGTCA GAGCATAAAT CTCATGACCC AGTAGTACAC
    CCTCATGGGT CAGGGCATAA CAAACATGAG CCAATAGTTA ATCCTATTGG GTCAGGGCAT
    AACTCCCATG GGTCAGTAGT GAATCCTCAT GGGTCAGAGC ATAACCCTCA TGGGTCAGTA
    GTGAATCCTC ATGGGTCAGA GCATAACCCT CATGGGTCAG TAGTGAATCC TCATGGGTCA
    GAGCATAACC CTCATGGGTC AGTAGTGAAT ACTCATGGCT CAGGGCATAA CTCTCATGGG
    TCAGTAGCAA ATCCTCATGG CTCAGAGAAT AACCCACATG GGTCAGAGCA TAACTCTCAT
    GGGTCAGTAG CAAATCCTCA TGGGTCAGGG CATACATTTC ATGGGTCAAT AGCAAATCCT
    CATGTGTCAG CGGAAAACCC TCATGGGTCA GTAGCGAATC CTCATGGGTC AGAGCATAAC
    TCTCATGGGT CAGTAGCGAA TCCTCACGGG TCAGAGCATA ACCCTCATGG GTCAGAAGCG
    AATCCTCATG GGTCAGAGCA TAACTCTCAT GGATCAGTAG CAAATCCTCA TGGGTCAGGG
    CATAAGCCAC ATGGGTCAGA GTATAACCCT CATGGGTCAG TAGCGAATCC TCTTGTGTCA
    GAGTATAACA CTCATGGGTC AGTAGCGAAT CCTCATGGGT CAGCGCATAA CCCTCATGGG
    TCAGTAGCGA ATCCTCATGG GTCAGGGCAT GACCCACATG GGTCAGAGTA TAACCCTCAT
    GGGTCAGTAG AAAACCCTCA GGGGTCAGCG CCTAACTCTC ATGGATCGGT AGCAAATCCT
    CATGGGTCAG TAGAAAATCC TCATGCGTCA GCGCATAACT CTCATGGATC GGTAGCAAAT
    CCTCATGGGT CAGCGCATAA CTCTCATGGG TCGGTAGCAA GTCCTCATGG GTCAGCGCAT
    AACTCTCATG GGTCAGGGCA TAATCCTCAT GGGTCAGGGC ATAATCCTCA TGAGTCAGGG
    CATAAAACTC ATGGGCCAAG GCATAAGCCT CATGGGTCAG TAGTGTATCC TCATGAGCCA
    ATGCATAACC CTCATGGACA CCTACATAAT GAACCAAAAA GTGAGTCACA TGGGCCGATA
    CCAGAGTCTG CCAGGACAAC AGACGAATCC GTGCTGCCAA TCTCAGCATT GGAGTCTGAT
    CCAAACATCG AGATGGTTTC AATACACATG TCACCTGAAG AGAAAAAGGA GGGATATACT
    CATAAAGCTC TTATGAAGTT CTTATTATCT GGCGGGAAAC AGACTGTACA GCTAAAAGGC
    ATCATATATG ACGACTTTCA AGATTGTGAA TGTAACGGAC ACTCGAATCG CTGCAGTTAC
    ATCGACTTCC TGAACATAGT GACGTGCGTG AGCTGCAAAC ACAACACGAG GGGGCAGAAC
    TGCGAACACT GTCGCATGAG CTACTACAAA AACATGTCTG CAGAGCCTGA TGATGAGAAC
    GTGTGTATCG AGTGTAACTG TAATGCGTTG GGCTCAGTTC ATGACCGCTG CAACGGCACC
    GGCTTCTGTC AGTGTAAAGA AGGCACAACC GGAGTCAAAT GTGATGAATG TCTCCCGGGC
    TACTATTGGA AACAGGGCTG TTTACCGAAC GTGTGCGACG ACGAGTTACT CTTGTGCCAG
    AACGGAGGGA CGTGCGTTCA GAATCAGCGC TGCATCTGCA CACCAGACTT TAAGGGTGTT
    TTGTGCCAAC ATTCGCGCTG TGAAGAAGGC AAAAAGTGCA ACGGCGCCAG CGCTCTCCGT
    TTGAGCCTGG CACTCCTGCT CTGCCCGCTT CTCGCCACCC TGCTGGGCTC CACCGCCTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293378.1
    CDS62..4624
    Translation

    Target ORF information:

    RefSeq Version XM_009295103.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio netrin g2b (ntng2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295103.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGCACCA
    CCAGTGGAGG ATTCTGGGGA AGTTGAAGAG ATTTCTGAAA AGAAAGAGAT GACTTCAGGG
    AGACATGAAG TATCTGAAGA TGTAAAGAGA GTGGAGACTT TTCTGGAACA TAACAGACAC
    AGGGAAGAAA CTGCACCTGA ACCTGTTGGA TCAGTGCGGG AGTCTGCCGA GCCAAAGCCA
    GAGTCGATTC CAAAGCCAAT TTTGGAAACA CACAAGCCAC TACACAAACC TCAAGTTCAT
    GTGTCTACAG CGGAATCTCA TGGAGCAGCA CAAACATCAC ATGAATCAGT AACAAAGCAT
    CATGAACCAA TTCAGAAACT TCATGAGTCA ACAGTGAAAC CTCACTCAAT GCATACAACC
    CATGAGACTA TACAGAAACC TCATGAACCA AAAGTGAATT CTCATGAATC AATGCATAAA
    CCCCATGAGC AAATTGTAAA TCTTAATGAA TCAATGTATA AGCCTCATCA GCCAATATGG
    GATCCACATT CATCGCCGCA AAAACCTTTT GAGCCAAACG TGAACCCCCT TGAGTCAGTA
    GTGAATATTC ATGAGTCAAC GCATAAATCA CATGAAGCAG TAGTGAATCC TCAGGAATCA
    ATGAATAACC CTCATGGGTC AGTAGTAAAC CCTCACAAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ACCCTCACGG GTCAGGGCAT AACCCTCATG GGTCAGTAGT CAACCCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAATCCTC ACGAGTCAGG GCATAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AATAGTAAAC
    CCTTATGAGT CAGGGCATAA CTCTTATGGG TCAGTAGTGA ACCCTCACGG GTCAGTAGTG
    AACCCTCACG GGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAAT AGTGAACCCT CACGGGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAAAC CTCACGGGTC AGTAGTGAAC CCTCACGAGT CAGGGCATAA CCCTCATGGA
    ACAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAATAGT AAACCCTCTT
    GAGTCAGGGC ACAACCCTCA TGGGTCGGTA GTGAATCCTC ATGGCTCAGG GCTTAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAC
    CCTCACGAGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ACCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAGTAGT GAACCCTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAACCCTC ACGAGTCAGG GCATAATCCT CATGGAACAG TAGTGAATCC TCACGAGTCA
    GGGCATAACC CTCATGGGTC AGTAGTGAAC CCTCACGAGT CAGGGCATAA TCCTCATGGA
    ACAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAATAGT AAACCCTCTT
    GATTCAGGGC ACAACCCTCA TGGGTCGGTA GTGAATCCTC ATGGCTCAGG GCTTAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAT
    CCTCACGAGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATTCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAGTAGT GAATACTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAATCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAG TAGTGAATAT TCATAGTTCA
    GAGCAGAAAT CTCATGATCC AGTAGGGAAT CCGCATGGGT CAGAGCATAA CCTTCATGGG
    TCAGAGGTGA ATCATCATGG ATCAGAACAT AAATCTCATG AGCCACTAGT AAACCCCCAT
    GGGTCAGAGC ATAGTCCTCA TAGGTCAGAA GTGGATCCTC ATGGGTCAGT AGTGAATCCT
    CATGGGTCAG GGCATAAATC TCATGACCCA GTTATGAATC CCCATGATCC AGTAGTAAAT
    CCTCATGGGT CAGAGCATAA ATCTCACGAT GCAGTAGTGA ATCATCATGG GTCAGTGGAT
    AAATCTCATG AACCAGTAGT GAATGCTCAT GGGTCAGAGC ATAAGCCTCA TGGGTCAGGT
    CATAAACCTC ATGAGTCAGT AGTGAATCCT CATGGATTGG AGCATAAATC TCATGACCTA
    GAAGTAAATC CTCATGGTTC AAAGCACAAC ACACATGAGC CAATAGTGAA TCCTCATGGG
    ACAGAGCATA ACCCTCATGG GTCATCAGCA AATCCTCATG AGTCAGATCA TAAATCTCAT
    GAGCCAGTAG TTAATCCACA TGGGTCAGGG CACAACCCTC ATGGGTCAGG GCATAACCCT
    CACAACCCAG TAGTGAATCC TCATGTGTCA GAGCATAAAT CTCATGACCC AGTAGTACAC
    CCTCATGGGT CAGGGCATAA CAAACATGAG CCAATAGTTA ATCCTATTGG GTCAGGGCAT
    AACTCCCATG GGTCAGTAGT GAATCCTCAT GGGTCAGAGC ATAACCCTCA TGGGTCAGTA
    GTGAATCCTC ATGGGTCAGA GCATAACCCT CATGGGTCAG TAGTGAATCC TCATGGGTCA
    GAGCATAACC CTCATGGGTC AGTAGTGAAT ACTCATGGCT CAGGGCATAA CTCTCATGGG
    TCAGTAGCAA ATCCTCATGG CTCAGAGAAT AACCCACATG GGTCAGAGCA TAACTCTCAT
    GGGTCAGTAG CAAATCCTCA TGGGTCAGGG CATACATTTC ATGGGTCAAT AGCAAATCCT
    CATGTGTCAG CGGAAAACCC TCATGGGTCA GTAGCGAATC CTCATGGGTC AGAGCATAAC
    TCTCATGGGT CAGTAGCGAA TCCTCACGGG TCAGAGCATA ACCCTCATGG GTCAGAAGCG
    AATCCTCATG GGTCAGAGCA TAACTCTCAT GGATCAGTAG CAAATCCTCA TGGGTCAGGG
    CATAAGCCAC ATGGGTCAGA GTATAACCCT CATGGGTCAG TAGCGAATCC TCTTGTGTCA
    GAGTATAACA CTCATGGGTC AGTAGCGAAT CCTCATGGGT CAGCGCATAA CCCTCATGGG
    TCAGTAGCGA ATCCTCATGG GTCAGGGCAT GACCCACATG GGTCAGAGTA TAACCCTCAT
    GGGTCAGTAG AAAACCCTCA GGGGTCAGCG CCTAACTCTC ATGGATCGGT AGCAAATCCT
    CATGGGTCAG TAGAAAATCC TCATGCGTCA GCGCATAACT CTCATGGATC GGTAGCAAAT
    CCTCATGGGT CAGCGCATAA CTCTCATGGG TCGGTAGCAA GTCCTCATGG GTCAGCGCAT
    AACTCTCATG GGTCAGGGCA TAATCCTCAT GGGTCAGGGC ATAATCCTCA TGAGTCAGGG
    CATAAAACTC ATGGGCCAAG GCATAAGCCT CATGGGTCAG TAGTGTATCC TCATGAGCCA
    ATGCATAACC CTCATGGACA CCTACATAAT GAACCAAAAA GTGAGTCACA TGGGCCGATA
    CCAGAGTCTG CCAGGACAAC AGACGAATCC GTGCTGCCAA TCTCAGCATT GGAGTCTGAT
    CCAAACATCG AGATGGTTTC AATACACATG TCACCTGAAG AGAAAAAGGA GGGATATACT
    CATAAAGCTC TTATGAAGTT CTTATTATCT GGCGGGAAAC AGACTGTACA GCTAAAAGGC
    ATCATATATG ACGACTTTCA AGATTGTGAA TGTAACGGAC ACTCGAATCG CTGCAGTTAC
    ATCGACTTCC TGAACATAGT GACGTGCGTG AGCTGCAAAC ACAACACGAG GGGGCAGAAC
    TGCGAACACT GTCGCATGAG CTACTACAAA AACATGTCTG CAGAGCCTGA TGATGAGAAC
    GTGTGTATCG AGTGTAACTG TAATGCGTTG GGCTCAGTTC ATGACCGCTG CAACGGCACC
    GGCTTCTGTC AGTGTAAAGA AGGCACAACC GGAGTCAAAT GTGATGAATG TCTCCCGGGC
    TACTATTGGA AACAGGGCTG TTTACCGAAC GTGTGCGACG ACGAGTTACT CTTGTGCCAG
    AACGGAGGGA CGTGCGTTCA GAATCAGCGC TGCATCTGCA CACCAGACTT TAAGGGTGTT
    TTGTGCCAAC ATTCGCGCTG TGAAGAAGGC AAAAAGTGCA ACGGCGCCAG CGCTCTCCGT
    TTGAGCCTGG CACTCCTGCT CTGCCCGCTT CTCGCCACCC TGCTGGGCTC CACCGCCTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa51521
    Clone ID Related Accession (Same CDS sequence) XM_009295103.3 , XM_009295103.2
    Accession Version XM_009295103.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product filaggrin-2-like isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295103.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGCACCA
    CCAGTGGAGG ATTCTGGGGA AGTTGAAGAG ATTTCTGAAA AGAAAGAGAT GACTTCAGGG
    AGACATGAAG TATCTGAAGA TGTAAAGAGA GTGGAGACTT TTCTGGAACA TAACAGACAC
    AGGGAAGAAA CTGCACCTGA ACCTGTTGGA TCAGTGCGGG AGTCTGCCGA GCCAAAGCCA
    GAGTCGATTC CAAAGCCAAT TTTGGAAACA CACAAGCCAC TACACAAACC TCAAGTTCAT
    GTGTCTACAG CGGAATCTCA TGGAGCAGCA CAAACATCAC ATGAATCAGT AACAAAGCAT
    CATGAACCAA TTCAGAAACT TCATGAGTCA ACAGTGAAAC CTCACTCAAT GCATACAACC
    CATGAGACTA TACAGAAACC TCATGAACCA AAAGTGAATT CTCATGAATC AATGCATAAA
    CCCCATGAGC AAATTGTAAA TCTTAATGAA TCAATGTATA AGCCTCATCA GCCAATATGG
    GATCCACATT CATCGCCGCA AAAACCTTTT GAGCCAAACG TGAACCCCCT TGAGTCAGTA
    GTGAATATTC ATGAGTCAAC GCATAAATCA CATGAAGCAG TAGTGAATCC TCAGGAATCA
    ATGAATAACC CTCATGGGTC AGTAGTAAAC CCTCACAAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ACCCTCACGG GTCAGGGCAT AACCCTCATG GGTCAGTAGT CAACCCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAATCCTC ACGAGTCAGG GCATAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AATAGTAAAC
    CCTTATGAGT CAGGGCATAA CTCTTATGGG TCAGTAGTGA ACCCTCACGG GTCAGTAGTG
    AACCCTCACG GGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAAT AGTGAACCCT CACGGGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAAAC CTCACGGGTC AGTAGTGAAC CCTCACGAGT CAGGGCATAA CCCTCATGGA
    ACAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAATAGT AAACCCTCTT
    GAGTCAGGGC ACAACCCTCA TGGGTCGGTA GTGAATCCTC ATGGCTCAGG GCTTAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAC
    CCTCACGAGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ACCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAGTAGT GAACCCTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAACCCTC ACGAGTCAGG GCATAATCCT CATGGAACAG TAGTGAATCC TCACGAGTCA
    GGGCATAACC CTCATGGGTC AGTAGTGAAC CCTCACGAGT CAGGGCATAA TCCTCATGGA
    ACAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAATAGT AAACCCTCTT
    GATTCAGGGC ACAACCCTCA TGGGTCGGTA GTGAATCCTC ATGGCTCAGG GCTTAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAT
    CCTCACGAGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATTCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAGTAGT GAATACTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAATCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAG TAGTGAATAT TCATAGTTCA
    GAGCAGAAAT CTCATGATCC AGTAGGGAAT CCGCATGGGT CAGAGCATAA CCTTCATGGG
    TCAGAGGTGA ATCATCATGG ATCAGAACAT AAATCTCATG AGCCACTAGT AAACCCCCAT
    GGGTCAGAGC ATAGTCCTCA TAGGTCAGAA GTGGATCCTC ATGGGTCAGT AGTGAATCCT
    CATGGGTCAG GGCATAAATC TCATGACCCA GTTATGAATC CCCATGATCC AGTAGTAAAT
    CCTCATGGGT CAGAGCATAA ATCTCACGAT GCAGTAGTGA ATCATCATGG GTCAGTGGAT
    AAATCTCATG AACCAGTAGT GAATGCTCAT GGGTCAGAGC ATAAGCCTCA TGGGTCAGGT
    CATAAACCTC ATGAGTCAGT AGTGAATCCT CATGGATTGG AGCATAAATC TCATGACCTA
    GAAGTAAATC CTCATGGTTC AAAGCACAAC ACACATGAGC CAATAGTGAA TCCTCATGGG
    ACAGAGCATA ACCCTCATGG GTCATCAGCA AATCCTCATG AGTCAGATCA TAAATCTCAT
    GAGCCAGTAG TTAATCCACA TGGGTCAGGG CACAACCCTC ATGGGTCAGG GCATAACCCT
    CACAACCCAG TAGTGAATCC TCATGTGTCA GAGCATAAAT CTCATGACCC AGTAGTACAC
    CCTCATGGGT CAGGGCATAA CAAACATGAG CCAATAGTTA ATCCTATTGG GTCAGGGCAT
    AACTCCCATG GGTCAGTAGT GAATCCTCAT GGGTCAGAGC ATAACCCTCA TGGGTCAGTA
    GTGAATCCTC ATGGGTCAGA GCATAACCCT CATGGGTCAG TAGTGAATCC TCATGGGTCA
    GAGCATAACC CTCATGGGTC AGTAGTGAAT ACTCATGGCT CAGGGCATAA CTCTCATGGG
    TCAGTAGCAA ATCCTCATGG CTCAGAGAAT AACCCACATG GGTCAGAGCA TAACTCTCAT
    GGGTCAGTAG CAAATCCTCA TGGGTCAGGG CATACATTTC ATGGGTCAAT AGCAAATCCT
    CATGTGTCAG CGGAAAACCC TCATGGGTCA GTAGCGAATC CTCATGGGTC AGAGCATAAC
    TCTCATGGGT CAGTAGCGAA TCCTCACGGG TCAGAGCATA ACCCTCATGG GTCAGAAGCG
    AATCCTCATG GGTCAGAGCA TAACTCTCAT GGATCAGTAG CAAATCCTCA TGGGTCAGGG
    CATAAGCCAC ATGGGTCAGA GTATAACCCT CATGGGTCAG TAGCGAATCC TCTTGTGTCA
    GAGTATAACA CTCATGGGTC AGTAGCGAAT CCTCATGGGT CAGCGCATAA CCCTCATGGG
    TCAGTAGCGA ATCCTCATGG GTCAGGGCAT GACCCACATG GGTCAGAGTA TAACCCTCAT
    GGGTCAGTAG AAAACCCTCA GGGGTCAGCG CCTAACTCTC ATGGATCGGT AGCAAATCCT
    CATGGGTCAG TAGAAAATCC TCATGCGTCA GCGCATAACT CTCATGGATC GGTAGCAAAT
    CCTCATGGGT CAGCGCATAA CTCTCATGGG TCGGTAGCAA GTCCTCATGG GTCAGCGCAT
    AACTCTCATG GGTCAGGGCA TAATCCTCAT GGGTCAGGGC ATAATCCTCA TGAGTCAGGG
    CATAAAACTC ATGGGCCAAG GCATAAGCCT CATGGGTCAG TAGTGTATCC TCATGAGCCA
    ATGCATAACC CTCATGGACA CCTACATAAT GAACCAAAAA GTGAGTCACA TGGGCCGATA
    CCAGAGTCTG CCAGGACAAC AGACGAATCC GTGCTGCCAA TCTCAGCATT GGAGTCTGAT
    CCAAACATCG AGATGGTTTC AATACACATG TCACCTGAAG AGAAAAAGGA GGGATATACT
    CATAAAGCTC TTATGAAGTT CTTATTATCT GGCGGGAAAC AGACTGTACA GCTAAAAGGC
    ATCATATATG ACGACTTTCA AGATTGTGAA TGTAACGGAC ACTCGAATCG CTGCAGTTAC
    ATCGACTTCC TGAACATAGT GACGTGCGTG AGCTGCAAAC ACAACACGAG GGGGCAGAAC
    TGCGAACACT GTCGCATGAG CTACTACAAA AACATGTCTG CAGAGCCTGA TGATGAGAAC
    GTGTGTATCG AGTGTAACTG TAATGCGTTG GGCTCAGTTC ATGACCGCTG CAACGGCACC
    GGCTTCTGTC AGTGTAAAGA AGGCACAACC GGAGTCAAAT GTGATGAATG TCTCCCGGGC
    TACTATTGGA AACAGGGCTG TTTACCGAAC GTGTGCGACG ACGAGTTACT CTTGTGCCAG
    AACGGAGGGA CGTGCGTTCA GAATCAGCGC TGCATCTGCA CACCAGACTT TAAGGGTGTT
    TTGTGCCAAC ATTCGCGCTG TGAAGAAGGC AAAAAGTGCA ACGGCGCCAG CGCTCTCCGT
    TTGAGCCTGG CACTCCTGCT CTGCCCGCTT CTCGCCACCC TGCTGGGCTC CACCGCCTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293378.1
    CDS60..4622
    Translation

    Target ORF information:

    RefSeq Version XM_009295103.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio netrin g2b (ntng2b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295103.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGCACCA
    CCAGTGGAGG ATTCTGGGGA AGTTGAAGAG ATTTCTGAAA AGAAAGAGAT GACTTCAGGG
    AGACATGAAG TATCTGAAGA TGTAAAGAGA GTGGAGACTT TTCTGGAACA TAACAGACAC
    AGGGAAGAAA CTGCACCTGA ACCTGTTGGA TCAGTGCGGG AGTCTGCCGA GCCAAAGCCA
    GAGTCGATTC CAAAGCCAAT TTTGGAAACA CACAAGCCAC TACACAAACC TCAAGTTCAT
    GTGTCTACAG CGGAATCTCA TGGAGCAGCA CAAACATCAC ATGAATCAGT AACAAAGCAT
    CATGAACCAA TTCAGAAACT TCATGAGTCA ACAGTGAAAC CTCACTCAAT GCATACAACC
    CATGAGACTA TACAGAAACC TCATGAACCA AAAGTGAATT CTCATGAATC AATGCATAAA
    CCCCATGAGC AAATTGTAAA TCTTAATGAA TCAATGTATA AGCCTCATCA GCCAATATGG
    GATCCACATT CATCGCCGCA AAAACCTTTT GAGCCAAACG TGAACCCCCT TGAGTCAGTA
    GTGAATATTC ATGAGTCAAC GCATAAATCA CATGAAGCAG TAGTGAATCC TCAGGAATCA
    ATGAATAACC CTCATGGGTC AGTAGTAAAC CCTCACAAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ACCCTCACGG GTCAGGGCAT AACCCTCATG GGTCAGTAGT CAACCCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAATCCTC ACGAGTCAGG GCATAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AATAGTAAAC
    CCTTATGAGT CAGGGCATAA CTCTTATGGG TCAGTAGTGA ACCCTCACGG GTCAGTAGTG
    AACCCTCACG GGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAAT AGTGAACCCT CACGGGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAAAC CTCACGGGTC AGTAGTGAAC CCTCACGAGT CAGGGCATAA CCCTCATGGA
    ACAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAATAGT AAACCCTCTT
    GAGTCAGGGC ACAACCCTCA TGGGTCGGTA GTGAATCCTC ATGGCTCAGG GCTTAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAC
    CCTCACGAGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ACCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAGTAGT GAACCCTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAACCCTC ACGAGTCAGG GCATAATCCT CATGGAACAG TAGTGAATCC TCACGAGTCA
    GGGCATAACC CTCATGGGTC AGTAGTGAAC CCTCACGAGT CAGGGCATAA TCCTCATGGA
    ACAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAATAGT AAACCCTCTT
    GATTCAGGGC ACAACCCTCA TGGGTCGGTA GTGAATCCTC ATGGCTCAGG GCTTAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAT
    CCTCACGAGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATTCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAGTAGT GAATACTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAATCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAG TAGTGAATAT TCATAGTTCA
    GAGCAGAAAT CTCATGATCC AGTAGGGAAT CCGCATGGGT CAGAGCATAA CCTTCATGGG
    TCAGAGGTGA ATCATCATGG ATCAGAACAT AAATCTCATG AGCCACTAGT AAACCCCCAT
    GGGTCAGAGC ATAGTCCTCA TAGGTCAGAA GTGGATCCTC ATGGGTCAGT AGTGAATCCT
    CATGGGTCAG GGCATAAATC TCATGACCCA GTTATGAATC CCCATGATCC AGTAGTAAAT
    CCTCATGGGT CAGAGCATAA ATCTCACGAT GCAGTAGTGA ATCATCATGG GTCAGTGGAT
    AAATCTCATG AACCAGTAGT GAATGCTCAT GGGTCAGAGC ATAAGCCTCA TGGGTCAGGT
    CATAAACCTC ATGAGTCAGT AGTGAATCCT CATGGATTGG AGCATAAATC TCATGACCTA
    GAAGTAAATC CTCATGGTTC AAAGCACAAC ACACATGAGC CAATAGTGAA TCCTCATGGG
    ACAGAGCATA ACCCTCATGG GTCATCAGCA AATCCTCATG AGTCAGATCA TAAATCTCAT
    GAGCCAGTAG TTAATCCACA TGGGTCAGGG CACAACCCTC ATGGGTCAGG GCATAACCCT
    CACAACCCAG TAGTGAATCC TCATGTGTCA GAGCATAAAT CTCATGACCC AGTAGTACAC
    CCTCATGGGT CAGGGCATAA CAAACATGAG CCAATAGTTA ATCCTATTGG GTCAGGGCAT
    AACTCCCATG GGTCAGTAGT GAATCCTCAT GGGTCAGAGC ATAACCCTCA TGGGTCAGTA
    GTGAATCCTC ATGGGTCAGA GCATAACCCT CATGGGTCAG TAGTGAATCC TCATGGGTCA
    GAGCATAACC CTCATGGGTC AGTAGTGAAT ACTCATGGCT CAGGGCATAA CTCTCATGGG
    TCAGTAGCAA ATCCTCATGG CTCAGAGAAT AACCCACATG GGTCAGAGCA TAACTCTCAT
    GGGTCAGTAG CAAATCCTCA TGGGTCAGGG CATACATTTC ATGGGTCAAT AGCAAATCCT
    CATGTGTCAG CGGAAAACCC TCATGGGTCA GTAGCGAATC CTCATGGGTC AGAGCATAAC
    TCTCATGGGT CAGTAGCGAA TCCTCACGGG TCAGAGCATA ACCCTCATGG GTCAGAAGCG
    AATCCTCATG GGTCAGAGCA TAACTCTCAT GGATCAGTAG CAAATCCTCA TGGGTCAGGG
    CATAAGCCAC ATGGGTCAGA GTATAACCCT CATGGGTCAG TAGCGAATCC TCTTGTGTCA
    GAGTATAACA CTCATGGGTC AGTAGCGAAT CCTCATGGGT CAGCGCATAA CCCTCATGGG
    TCAGTAGCGA ATCCTCATGG GTCAGGGCAT GACCCACATG GGTCAGAGTA TAACCCTCAT
    GGGTCAGTAG AAAACCCTCA GGGGTCAGCG CCTAACTCTC ATGGATCGGT AGCAAATCCT
    CATGGGTCAG TAGAAAATCC TCATGCGTCA GCGCATAACT CTCATGGATC GGTAGCAAAT
    CCTCATGGGT CAGCGCATAA CTCTCATGGG TCGGTAGCAA GTCCTCATGG GTCAGCGCAT
    AACTCTCATG GGTCAGGGCA TAATCCTCAT GGGTCAGGGC ATAATCCTCA TGAGTCAGGG
    CATAAAACTC ATGGGCCAAG GCATAAGCCT CATGGGTCAG TAGTGTATCC TCATGAGCCA
    ATGCATAACC CTCATGGACA CCTACATAAT GAACCAAAAA GTGAGTCACA TGGGCCGATA
    CCAGAGTCTG CCAGGACAAC AGACGAATCC GTGCTGCCAA TCTCAGCATT GGAGTCTGAT
    CCAAACATCG AGATGGTTTC AATACACATG TCACCTGAAG AGAAAAAGGA GGGATATACT
    CATAAAGCTC TTATGAAGTT CTTATTATCT GGCGGGAAAC AGACTGTACA GCTAAAAGGC
    ATCATATATG ACGACTTTCA AGATTGTGAA TGTAACGGAC ACTCGAATCG CTGCAGTTAC
    ATCGACTTCC TGAACATAGT GACGTGCGTG AGCTGCAAAC ACAACACGAG GGGGCAGAAC
    TGCGAACACT GTCGCATGAG CTACTACAAA AACATGTCTG CAGAGCCTGA TGATGAGAAC
    GTGTGTATCG AGTGTAACTG TAATGCGTTG GGCTCAGTTC ATGACCGCTG CAACGGCACC
    GGCTTCTGTC AGTGTAAAGA AGGCACAACC GGAGTCAAAT GTGATGAATG TCTCCCGGGC
    TACTATTGGA AACAGGGCTG TTTACCGAAC GTGTGCGACG ACGAGTTACT CTTGTGCCAG
    AACGGAGGGA CGTGCGTTCA GAATCAGCGC TGCATCTGCA CACCAGACTT TAAGGGTGTT
    TTGTGCCAAC ATTCGCGCTG TGAAGAAGGC AAAAAGTGCA ACGGCGCCAG CGCTCTCCGT
    TTGAGCCTGG CACTCCTGCT CTGCCCGCTT CTCGCCACCC TGCTGGGCTC CACCGCCTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa51523
    Clone ID Related Accession (Same CDS sequence) XM_021468964.1
    Accession Version XM_021468964.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4509bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product filaggrin-2-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGATGCT
    CCTCCGGCAC ATGTAGATAC TGTAAACCAC GTCCCATCAG CTGAAATAGA CCCCAAGGCA
    CTTTATACCA CCCCAAAAGC CACGCCCCCT TCTCATATTT TCAGAGAGGA CACTCCCCGC
    ACGGCCACAC CTCCAGAGGA GGAGCCTGTG CACATACATA TACAGAGCAA AGCACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTGAA TCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAATAGTG AACCCTCACG GGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAAACCTCA CGGGTCAGTA GTGAACCCTC ACGAGTCAGG GCATAACCCT
    CATGGAACAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AATAGTAAAC
    CCTCTTGAGT CAGGGCACAA CCCTCATGGG TCGGTAGTGA ATCCTCATGG CTCAGGGCTT
    AACCCTCATG GGTCAGTAGT GAATCCTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAACCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAG TAGTGAACCC TCACGAGTCA
    GGGCATAACC CTCATGGGTC AGTAGTGAAC CCTCACGAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ACCCTCACGA GTCAGGGCAT AATCCTCATG GAACAGTAGT GAATCCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAACCCTC ACGAGTCAGG GCATAATCCT
    CATGGAACAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AATAGTAAAC
    CCTCTTGATT CAGGGCACAA CCCTCATGGG TCGGTAGTGA ATCCTCATGG CTCAGGGCTT
    AACCCTCATG GGTCAGTAGT GAATCCTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAATCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAG TAGTGAATTC TCACGAGTCA
    GGGCATAACC CTCATGGGTC AGTAGTGAAT ACTCACGAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAGTAGT GAATATTCAT
    AGTTCAGAGC AGAAATCTCA TGATCCAGTA GGGAATCCGC ATGGGTCAGA GCATAACCTT
    CATGGGTCAG AGGTGAATCA TCATGGATCA GAACATAAAT CTCATGAGCC ACTAGTAAAC
    CCCCATGGGT CAGAGCATAG TCCTCATAGG TCAGAAGTGG ATCCTCATGG GTCAGTAGTG
    AATCCTCATG GGTCAGGGCA TAAATCTCAT GACCCAGTTA TGAATCCCCA TGATCCAGTA
    GTAAATCCTC ATGGGTCAGA GCATAAATCT CACGATGCAG TAGTGAATCA TCATGGGTCA
    GTGGATAAAT CTCATGAACC AGTAGTGAAT GCTCATGGGT CAGAGCATAA GCCTCATGGG
    TCAGGTCATA AACCTCATGA GTCAGTAGTG AATCCTCATG GATTGGAGCA TAAATCTCAT
    GACCTAGAAG TAAATCCTCA TGGTTCAAAG CACAACACAC ATGAGCCAAT AGTGAATCCT
    CATGGGACAG AGCATAACCC TCATGGGTCA TCAGCAAATC CTCATGAGTC AGATCATAAA
    TCTCATGAGC CAGTAGTTAA TCCACATGGG TCAGGGCACA ACCCTCATGG GTCAGGGCAT
    AACCCTCACA ACCCAGTAGT GAATCCTCAT GTGTCAGAGC ATAAATCTCA TGACCCAGTA
    GTACACCCTC ATGGGTCAGG GCATAACAAA CATGAGCCAA TAGTTAATCC TATTGGGTCA
    GGGCATAACT CCCATGGGTC AGTAGTGAAT CCTCATGGGT CAGAGCATAA CCCTCATGGG
    TCAGTAGTGA ATCCTCATGG GTCAGAGCAT AACCCTCATG GGTCAGTAGT GAATCCTCAT
    GGGTCAGAGC ATAACCCTCA TGGGTCAGTA GTGAATACTC ATGGCTCAGG GCATAACTCT
    CATGGGTCAG TAGCAAATCC TCATGGCTCA GAGAATAACC CACATGGGTC AGAGCATAAC
    TCTCATGGGT CAGTAGCAAA TCCTCATGGG TCAGGGCATA CATTTCATGG GTCAATAGCA
    AATCCTCATG TGTCAGCGGA AAACCCTCAT GGGTCAGTAG CGAATCCTCA TGGGTCAGAG
    CATAACTCTC ATGGGTCAGT AGCGAATCCT CACGGGTCAG AGCATAACCC TCATGGGTCA
    GAAGCGAATC CTCATGGGTC AGAGCATAAC TCTCATGGAT CAGTAGCAAA TCCTCATGGG
    TCAGGGCATA AGCCACATGG GTCAGAGTAT AACCCTCATG GGTCAGTAGC GAATCCTCTT
    GTGTCAGAGT ATAACACTCA TGGGTCAGTA GCGAATCCTC ATGGGTCAGC GCATAACCCT
    CATGGGTCAG TAGCGAATCC TCATGGGTCA GGGCATGACC CACATGGGTC AGAGTATAAC
    CCTCATGGGT CAGTAGAAAA CCCTCAGGGG TCAGCGCCTA ACTCTCATGG ATCGGTAGCA
    AATCCTCATG GGTCAGTAGA AAATCCTCAT GCGTCAGCGC ATAACTCTCA TGGATCGGTA
    GCAAATCCTC ATGGGTCAGC GCATAACTCT CATGGGTCGG TAGCAAGTCC TCATGGGTCA
    GCGCATAACT CTCATGGGTC AGGGCATAAT CCTCATGGGT CAGGGCATAA TCCTCATGAG
    TCAGGGCATA AAACTCATGG GCCAAGGCAT AAGCCTCATG GGTCAGTAGT GTATCCTCAT
    GAGCCAATGC ATAACCCTCA TGGACACCTA CATAATGAAC CAAAAAGTGA GTCACATGGG
    CCGATACCAG AGTCTGCCAG GACAACAGAC GAATCCGTGC TGCCAATCTC AGCATTGGAG
    TCTGATCCAA ACATCGAGAT GGTTTCAATA CACATGTCAC CTGAAGAGAA AAAGGAGGGA
    TATACTCATA AAGCTCTTAT GAAGTTCTTA TTATCTGGCG GGAAACAGAC TGTACAGCTA
    AAAGGCATCA TATATGACGA CTTTCAAGAT TGTGAATGTA ACGGACACTC GAATCGCTGC
    AGTTACATCG ACTTCCTGAA CATAGTGACG TGCGTGAGCT GCAAACACAA CACGAGGGGG
    CAGAACTGCG AACACTGTCG CATGAGCTAC TACAAAAACA TGTCTGCAGA GCCTGATGAT
    GAGAACGTGT GTATCGAGTG TAACTGTAAT GCGTTGGGCT CAGTTCATGA CCGCTGCAAC
    GGCACCGGCT TCTGTCAGTG TAAAGAAGGC ACAACCGGAG TCAAATGTGA TGAATGTCTC
    CCGGGCTACT ATTGGAAACA GGGCTGTTTA CCGAACGTGT GCGACGACGA GTTACTCTTG
    TGCCAGAACG GAGGGACGTG CGTTCAGAAT CAGCGCTGCA TCTGCACACC AGACTTTAAG
    GGTGTTTTGT GCCAACATTC GCGCTGTGAA GAAGGCAAAA AGTGCAACGG CGCCAGCGCT
    CTCCGTTTGA GCCTGGCACT CCTGCTCTGC CCGCTTCTCG CCACCCTGCT GGGCTCCACC
    GCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324639.1
    CDS62..4570
    Translation

    Target ORF information:

    RefSeq Version XM_021468964.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio netrin g2b (ntng2b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468964.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CATGACCGAT
    ACCTCAGCTG AAGAGTTCAC TACAGTTGTA ACAGAATCGC CAGTGTTGGA CACAGATGCT
    CCTCCGGCAC ATGTAGATAC TGTAAACCAC GTCCCATCAG CTGAAATAGA CCCCAAGGCA
    CTTTATACCA CCCCAAAAGC CACGCCCCCT TCTCATATTT TCAGAGAGGA CACTCCCCGC
    ACGGCCACAC CTCCAGAGGA GGAGCCTGTG CACATACATA TACAGAGCAA AGCACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTGAA TCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAATAGTG AACCCTCACG GGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAAACCTCA CGGGTCAGTA GTGAACCCTC ACGAGTCAGG GCATAACCCT
    CATGGAACAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AATAGTAAAC
    CCTCTTGAGT CAGGGCACAA CCCTCATGGG TCGGTAGTGA ATCCTCATGG CTCAGGGCTT
    AACCCTCATG GGTCAGTAGT GAATCCTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAACCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAG TAGTGAACCC TCACGAGTCA
    GGGCATAACC CTCATGGGTC AGTAGTGAAC CCTCACGAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ACCCTCACGA GTCAGGGCAT AATCCTCATG GAACAGTAGT GAATCCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAACCCTC ACGAGTCAGG GCATAATCCT
    CATGGAACAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AATAGTAAAC
    CCTCTTGATT CAGGGCACAA CCCTCATGGG TCGGTAGTGA ATCCTCATGG CTCAGGGCTT
    AACCCTCATG GGTCAGTAGT GAATCCTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAATCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAG TAGTGAATTC TCACGAGTCA
    GGGCATAACC CTCATGGGTC AGTAGTGAAT ACTCACGAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAGTAGT GAATATTCAT
    AGTTCAGAGC AGAAATCTCA TGATCCAGTA GGGAATCCGC ATGGGTCAGA GCATAACCTT
    CATGGGTCAG AGGTGAATCA TCATGGATCA GAACATAAAT CTCATGAGCC ACTAGTAAAC
    CCCCATGGGT CAGAGCATAG TCCTCATAGG TCAGAAGTGG ATCCTCATGG GTCAGTAGTG
    AATCCTCATG GGTCAGGGCA TAAATCTCAT GACCCAGTTA TGAATCCCCA TGATCCAGTA
    GTAAATCCTC ATGGGTCAGA GCATAAATCT CACGATGCAG TAGTGAATCA TCATGGGTCA
    GTGGATAAAT CTCATGAACC AGTAGTGAAT GCTCATGGGT CAGAGCATAA GCCTCATGGG
    TCAGGTCATA AACCTCATGA GTCAGTAGTG AATCCTCATG GATTGGAGCA TAAATCTCAT
    GACCTAGAAG TAAATCCTCA TGGTTCAAAG CACAACACAC ATGAGCCAAT AGTGAATCCT
    CATGGGACAG AGCATAACCC TCATGGGTCA TCAGCAAATC CTCATGAGTC AGATCATAAA
    TCTCATGAGC CAGTAGTTAA TCCACATGGG TCAGGGCACA ACCCTCATGG GTCAGGGCAT
    AACCCTCACA ACCCAGTAGT GAATCCTCAT GTGTCAGAGC ATAAATCTCA TGACCCAGTA
    GTACACCCTC ATGGGTCAGG GCATAACAAA CATGAGCCAA TAGTTAATCC TATTGGGTCA
    GGGCATAACT CCCATGGGTC AGTAGTGAAT CCTCATGGGT CAGAGCATAA CCCTCATGGG
    TCAGTAGTGA ATCCTCATGG GTCAGAGCAT AACCCTCATG GGTCAGTAGT GAATCCTCAT
    GGGTCAGAGC ATAACCCTCA TGGGTCAGTA GTGAATACTC ATGGCTCAGG GCATAACTCT
    CATGGGTCAG TAGCAAATCC TCATGGCTCA GAGAATAACC CACATGGGTC AGAGCATAAC
    TCTCATGGGT CAGTAGCAAA TCCTCATGGG TCAGGGCATA CATTTCATGG GTCAATAGCA
    AATCCTCATG TGTCAGCGGA AAACCCTCAT GGGTCAGTAG CGAATCCTCA TGGGTCAGAG
    CATAACTCTC ATGGGTCAGT AGCGAATCCT CACGGGTCAG AGCATAACCC TCATGGGTCA
    GAAGCGAATC CTCATGGGTC AGAGCATAAC TCTCATGGAT CAGTAGCAAA TCCTCATGGG
    TCAGGGCATA AGCCACATGG GTCAGAGTAT AACCCTCATG GGTCAGTAGC GAATCCTCTT
    GTGTCAGAGT ATAACACTCA TGGGTCAGTA GCGAATCCTC ATGGGTCAGC GCATAACCCT
    CATGGGTCAG TAGCGAATCC TCATGGGTCA GGGCATGACC CACATGGGTC AGAGTATAAC
    CCTCATGGGT CAGTAGAAAA CCCTCAGGGG TCAGCGCCTA ACTCTCATGG ATCGGTAGCA
    AATCCTCATG GGTCAGTAGA AAATCCTCAT GCGTCAGCGC ATAACTCTCA TGGATCGGTA
    GCAAATCCTC ATGGGTCAGC GCATAACTCT CATGGGTCGG TAGCAAGTCC TCATGGGTCA
    GCGCATAACT CTCATGGGTC AGGGCATAAT CCTCATGGGT CAGGGCATAA TCCTCATGAG
    TCAGGGCATA AAACTCATGG GCCAAGGCAT AAGCCTCATG GGTCAGTAGT GTATCCTCAT
    GAGCCAATGC ATAACCCTCA TGGACACCTA CATAATGAAC CAAAAAGTGA GTCACATGGG
    CCGATACCAG AGTCTGCCAG GACAACAGAC GAATCCGTGC TGCCAATCTC AGCATTGGAG
    TCTGATCCAA ACATCGAGAT GGTTTCAATA CACATGTCAC CTGAAGAGAA AAAGGAGGGA
    TATACTCATA AAGCTCTTAT GAAGTTCTTA TTATCTGGCG GGAAACAGAC TGTACAGCTA
    AAAGGCATCA TATATGACGA CTTTCAAGAT TGTGAATGTA ACGGACACTC GAATCGCTGC
    AGTTACATCG ACTTCCTGAA CATAGTGACG TGCGTGAGCT GCAAACACAA CACGAGGGGG
    CAGAACTGCG AACACTGTCG CATGAGCTAC TACAAAAACA TGTCTGCAGA GCCTGATGAT
    GAGAACGTGT GTATCGAGTG TAACTGTAAT GCGTTGGGCT CAGTTCATGA CCGCTGCAAC
    GGCACCGGCT TCTGTCAGTG TAAAGAAGGC ACAACCGGAG TCAAATGTGA TGAATGTCTC
    CCGGGCTACT ATTGGAAACA GGGCTGTTTA CCGAACGTGT GCGACGACGA GTTACTCTTG
    TGCCAGAACG GAGGGACGTG CGTTCAGAAT CAGCGCTGCA TCTGCACACC AGACTTTAAG
    GGTGTTTTGT GCCAACATTC GCGCTGTGAA GAAGGCAAAA AGTGCAACGG CGCCAGCGCT
    CTCCGTTTGA GCCTGGCACT CCTGCTCTGC CCGCTTCTCG CCACCCTGCT GGGCTCCACC
    GCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56957
    Clone ID Related Accession (Same CDS sequence) XM_017353009.2 , XM_017353009.1
    Accession Version XM_017353009.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product filaggrin-2-like isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CACACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTCAA CCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAGTAGTG AATCCTCACG AGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAAT AGTAAACCCT
    TATGAGTCAG GGCATAACTC TTATGGGTCA GTAGTGAACC CTCACGGGTC AGTAGTGAAC
    CCTCACGGGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAATAGT GAACCCTCAC GGGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAAACCTC ACGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAACCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAG
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAACCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAACC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA CCCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AACCCTCACG AGTCAGGGCA TAATCCTCAT GGAACAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAATCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAT
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATT CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA TACTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATATTCA TAGTTCAGAG
    CAGAAATCTC ATGATCCAGT AGGGAATCCG CATGGGTCAG AGCATAACCT TCATGGGTCA
    GAGGTGAATC ATCATGGATC AGAACATAAA TCTCATGAGC CACTAGTAAA CCCCCATGGG
    TCAGAGCATA GTCCTCATAG GTCAGAAGTG GATCCTCATG GGTCAGTAGT GAATCCTCAT
    GGGTCAGGGC ATAAATCTCA TGACCCAGTT ATGAATCCCC ATGATCCAGT AGTAAATCCT
    CATGGGTCAG AGCATAAATC TCACGATGCA GTAGTGAATC ATCATGGGTC AGTGGATAAA
    TCTCATGAAC CAGTAGTGAA TGCTCATGGG TCAGAGCATA AGCCTCATGG GTCAGGTCAT
    AAACCTCATG AGTCAGTAGT GAATCCTCAT GGATTGGAGC ATAAATCTCA TGACCTAGAA
    GTAAATCCTC ATGGTTCAAA GCACAACACA CATGAGCCAA TAGTGAATCC TCATGGGACA
    GAGCATAACC CTCATGGGTC ATCAGCAAAT CCTCATGAGT CAGATCATAA ATCTCATGAG
    CCAGTAGTTA ATCCACATGG GTCAGGGCAC AACCCTCATG GGTCAGGGCA TAACCCTCAC
    AACCCAGTAG TGAATCCTCA TGTGTCAGAG CATAAATCTC ATGACCCAGT AGTACACCCT
    CATGGGTCAG GGCATAACAA ACATGAGCCA ATAGTTAATC CTATTGGGTC AGGGCATAAC
    TCCCATGGGT CAGTAGTGAA TCCTCATGGG TCAGAGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCATG GGTCAGAGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA TGGGTCAGAG
    CATAACCCTC ATGGGTCAGT AGTGAATACT CATGGCTCAG GGCATAACTC TCATGGGTCA
    GTAGCAAATC CTCATGGCTC AGAGAATAAC CCACATGGGT CAGAGCATAA CTCTCATGGG
    TCAGTAGCAA ATCCTCATGG GTCAGGGCAT ACATTTCATG GGTCAATAGC AAATCCTCAT
    GTGTCAGCGG AAAACCCTCA TGGGTCAGTA GCGAATCCTC ATGGGTCAGA GCATAACTCT
    CATGGGTCAG TAGCGAATCC TCACGGGTCA GAGCATAACC CTCATGGGTC AGAAGCGAAT
    CCTCATGGGT CAGAGCATAA CTCTCATGGA TCAGTAGCAA ATCCTCATGG GTCAGGGCAT
    AAGCCACATG GGTCAGAGTA TAACCCTCAT GGGTCAGTAG CGAATCCTCT TGTGTCAGAG
    TATAACACTC ATGGGTCAGT AGCGAATCCT CATGGGTCAG CGCATAACCC TCATGGGTCA
    GTAGCGAATC CTCATGGGTC AGGGCATGAC CCACATGGGT CAGAGTATAA CCCTCATGGG
    TCAGTAGAAA ACCCTCAGGG GTCAGCGCCT AACTCTCATG GATCGGTAGC AAATCCTCAT
    GGGTCAGTAG AAAATCCTCA TGCGTCAGCG CATAACTCTC ATGGATCGGT AGCAAATCCT
    CATGGGTCAG CGCATAACTC TCATGGGTCG GTAGCAAGTC CTCATGGGTC AGCGCATAAC
    TCTCATGGGT CAGGGCATAA TCCTCATGGG TCAGGGCATA ATCCTCATGA GTCAGGGCAT
    AAAACTCATG GGCCAAGGCA TAAGCCTCAT GGGTCAGTAG TGTATCCTCA TGAGCCAATG
    CATAACCCTC ATGGACACCT ACATAATGAA CCAAAAAGTG AGTCACATGG GCCGATACCA
    GAGTCTGCCA GGACAACAGA CGAATCCGTG CTGCCAATCT CAGCATTGGA GTCTGATCCA
    AACATCGAGA TGGTTTCAAT ACACATGTCA CCTGAAGAGA AAAAGGAGGG ATATACTCAT
    AAAGCTCTTA TGAAGTTCTT ATTATCTGGC GGGAAACAGA CTGTACAGCT AAAAGGCATC
    ATATATGACG ACTTTCAAGA TTGTGAATGT AACGGACACT CGAATCGCTG CAGTTACATC
    GACTTCCTGA ACATAGTGAC GTGCGTGAGC TGCAAACACA ACACGAGGGG GCAGAACTGC
    GAACACTGTC GCATGAGCTA CTACAAAAAC ATGTCTGCAG AGCCTGATGA TGAGAACGTG
    TGTATCGAGT GTAACTGTAA TGCGTTGGGC TCAGTTCATG ACCGCTGCAA CGGCACCGGC
    TTCTGTCAGT GTAAAGAAGG CACAACCGGA GTCAAATGTG ATGAATGTCT CCCGGGCTAC
    TATTGGAAAC AGGGCTGTTT ACCGAACGTG TGCGACGACG AGTTACTCTT GTGCCAGAAC
    GGAGGGACGT GCGTTCAGAA TCAGCGCTGC ATCTGCACAC CAGACTTTAA GGGTGTTTTG
    TGCCAACATT CGCGCTGTGA AGAAGGCAAA AAGTGCAACG GCGCCAGCGC TCTCCGTTTG
    AGCCTGGCAC TCCTGCTCTG CCCGCTTCTC GCCACCCTGC TGGGCTCCAC CGCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208498.1
    CDS62..4561
    Translation

    Target ORF information:

    RefSeq Version XM_017353009.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio netrin g2b (ntng2b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353009.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CACACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTCAA CCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAGTAGTG AATCCTCACG AGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAAT AGTAAACCCT
    TATGAGTCAG GGCATAACTC TTATGGGTCA GTAGTGAACC CTCACGGGTC AGTAGTGAAC
    CCTCACGGGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAATAGT GAACCCTCAC GGGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAAACCTC ACGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAACCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAG
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAACCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAACC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA CCCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AACCCTCACG AGTCAGGGCA TAATCCTCAT GGAACAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAATCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAT
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATT CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA TACTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATATTCA TAGTTCAGAG
    CAGAAATCTC ATGATCCAGT AGGGAATCCG CATGGGTCAG AGCATAACCT TCATGGGTCA
    GAGGTGAATC ATCATGGATC AGAACATAAA TCTCATGAGC CACTAGTAAA CCCCCATGGG
    TCAGAGCATA GTCCTCATAG GTCAGAAGTG GATCCTCATG GGTCAGTAGT GAATCCTCAT
    GGGTCAGGGC ATAAATCTCA TGACCCAGTT ATGAATCCCC ATGATCCAGT AGTAAATCCT
    CATGGGTCAG AGCATAAATC TCACGATGCA GTAGTGAATC ATCATGGGTC AGTGGATAAA
    TCTCATGAAC CAGTAGTGAA TGCTCATGGG TCAGAGCATA AGCCTCATGG GTCAGGTCAT
    AAACCTCATG AGTCAGTAGT GAATCCTCAT GGATTGGAGC ATAAATCTCA TGACCTAGAA
    GTAAATCCTC ATGGTTCAAA GCACAACACA CATGAGCCAA TAGTGAATCC TCATGGGACA
    GAGCATAACC CTCATGGGTC ATCAGCAAAT CCTCATGAGT CAGATCATAA ATCTCATGAG
    CCAGTAGTTA ATCCACATGG GTCAGGGCAC AACCCTCATG GGTCAGGGCA TAACCCTCAC
    AACCCAGTAG TGAATCCTCA TGTGTCAGAG CATAAATCTC ATGACCCAGT AGTACACCCT
    CATGGGTCAG GGCATAACAA ACATGAGCCA ATAGTTAATC CTATTGGGTC AGGGCATAAC
    TCCCATGGGT CAGTAGTGAA TCCTCATGGG TCAGAGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCATG GGTCAGAGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA TGGGTCAGAG
    CATAACCCTC ATGGGTCAGT AGTGAATACT CATGGCTCAG GGCATAACTC TCATGGGTCA
    GTAGCAAATC CTCATGGCTC AGAGAATAAC CCACATGGGT CAGAGCATAA CTCTCATGGG
    TCAGTAGCAA ATCCTCATGG GTCAGGGCAT ACATTTCATG GGTCAATAGC AAATCCTCAT
    GTGTCAGCGG AAAACCCTCA TGGGTCAGTA GCGAATCCTC ATGGGTCAGA GCATAACTCT
    CATGGGTCAG TAGCGAATCC TCACGGGTCA GAGCATAACC CTCATGGGTC AGAAGCGAAT
    CCTCATGGGT CAGAGCATAA CTCTCATGGA TCAGTAGCAA ATCCTCATGG GTCAGGGCAT
    AAGCCACATG GGTCAGAGTA TAACCCTCAT GGGTCAGTAG CGAATCCTCT TGTGTCAGAG
    TATAACACTC ATGGGTCAGT AGCGAATCCT CATGGGTCAG CGCATAACCC TCATGGGTCA
    GTAGCGAATC CTCATGGGTC AGGGCATGAC CCACATGGGT CAGAGTATAA CCCTCATGGG
    TCAGTAGAAA ACCCTCAGGG GTCAGCGCCT AACTCTCATG GATCGGTAGC AAATCCTCAT
    GGGTCAGTAG AAAATCCTCA TGCGTCAGCG CATAACTCTC ATGGATCGGT AGCAAATCCT
    CATGGGTCAG CGCATAACTC TCATGGGTCG GTAGCAAGTC CTCATGGGTC AGCGCATAAC
    TCTCATGGGT CAGGGCATAA TCCTCATGGG TCAGGGCATA ATCCTCATGA GTCAGGGCAT
    AAAACTCATG GGCCAAGGCA TAAGCCTCAT GGGTCAGTAG TGTATCCTCA TGAGCCAATG
    CATAACCCTC ATGGACACCT ACATAATGAA CCAAAAAGTG AGTCACATGG GCCGATACCA
    GAGTCTGCCA GGACAACAGA CGAATCCGTG CTGCCAATCT CAGCATTGGA GTCTGATCCA
    AACATCGAGA TGGTTTCAAT ACACATGTCA CCTGAAGAGA AAAAGGAGGG ATATACTCAT
    AAAGCTCTTA TGAAGTTCTT ATTATCTGGC GGGAAACAGA CTGTACAGCT AAAAGGCATC
    ATATATGACG ACTTTCAAGA TTGTGAATGT AACGGACACT CGAATCGCTG CAGTTACATC
    GACTTCCTGA ACATAGTGAC GTGCGTGAGC TGCAAACACA ACACGAGGGG GCAGAACTGC
    GAACACTGTC GCATGAGCTA CTACAAAAAC ATGTCTGCAG AGCCTGATGA TGAGAACGTG
    TGTATCGAGT GTAACTGTAA TGCGTTGGGC TCAGTTCATG ACCGCTGCAA CGGCACCGGC
    TTCTGTCAGT GTAAAGAAGG CACAACCGGA GTCAAATGTG ATGAATGTCT CCCGGGCTAC
    TATTGGAAAC AGGGCTGTTT ACCGAACGTG TGCGACGACG AGTTACTCTT GTGCCAGAAC
    GGAGGGACGT GCGTTCAGAA TCAGCGCTGC ATCTGCACAC CAGACTTTAA GGGTGTTTTG
    TGCCAACATT CGCGCTGTGA AGAAGGCAAA AAGTGCAACG GCGCCAGCGC TCTCCGTTTG
    AGCCTGGCAC TCCTGCTCTG CCCGCTTCTC GCCACCCTGC TGGGCTCCAC CGCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56957
    Clone ID Related Accession (Same CDS sequence) XM_017353009.2 , XM_017353009.1
    Accession Version XM_017353009.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product filaggrin-2-like isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CACACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTCAA CCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAGTAGTG AATCCTCACG AGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAAT AGTAAACCCT
    TATGAGTCAG GGCATAACTC TTATGGGTCA GTAGTGAACC CTCACGGGTC AGTAGTGAAC
    CCTCACGGGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAATAGT GAACCCTCAC GGGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAAACCTC ACGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAACCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAG
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAACCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAACC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA CCCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AACCCTCACG AGTCAGGGCA TAATCCTCAT GGAACAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAATCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAT
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATT CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA TACTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATATTCA TAGTTCAGAG
    CAGAAATCTC ATGATCCAGT AGGGAATCCG CATGGGTCAG AGCATAACCT TCATGGGTCA
    GAGGTGAATC ATCATGGATC AGAACATAAA TCTCATGAGC CACTAGTAAA CCCCCATGGG
    TCAGAGCATA GTCCTCATAG GTCAGAAGTG GATCCTCATG GGTCAGTAGT GAATCCTCAT
    GGGTCAGGGC ATAAATCTCA TGACCCAGTT ATGAATCCCC ATGATCCAGT AGTAAATCCT
    CATGGGTCAG AGCATAAATC TCACGATGCA GTAGTGAATC ATCATGGGTC AGTGGATAAA
    TCTCATGAAC CAGTAGTGAA TGCTCATGGG TCAGAGCATA AGCCTCATGG GTCAGGTCAT
    AAACCTCATG AGTCAGTAGT GAATCCTCAT GGATTGGAGC ATAAATCTCA TGACCTAGAA
    GTAAATCCTC ATGGTTCAAA GCACAACACA CATGAGCCAA TAGTGAATCC TCATGGGACA
    GAGCATAACC CTCATGGGTC ATCAGCAAAT CCTCATGAGT CAGATCATAA ATCTCATGAG
    CCAGTAGTTA ATCCACATGG GTCAGGGCAC AACCCTCATG GGTCAGGGCA TAACCCTCAC
    AACCCAGTAG TGAATCCTCA TGTGTCAGAG CATAAATCTC ATGACCCAGT AGTACACCCT
    CATGGGTCAG GGCATAACAA ACATGAGCCA ATAGTTAATC CTATTGGGTC AGGGCATAAC
    TCCCATGGGT CAGTAGTGAA TCCTCATGGG TCAGAGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCATG GGTCAGAGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA TGGGTCAGAG
    CATAACCCTC ATGGGTCAGT AGTGAATACT CATGGCTCAG GGCATAACTC TCATGGGTCA
    GTAGCAAATC CTCATGGCTC AGAGAATAAC CCACATGGGT CAGAGCATAA CTCTCATGGG
    TCAGTAGCAA ATCCTCATGG GTCAGGGCAT ACATTTCATG GGTCAATAGC AAATCCTCAT
    GTGTCAGCGG AAAACCCTCA TGGGTCAGTA GCGAATCCTC ATGGGTCAGA GCATAACTCT
    CATGGGTCAG TAGCGAATCC TCACGGGTCA GAGCATAACC CTCATGGGTC AGAAGCGAAT
    CCTCATGGGT CAGAGCATAA CTCTCATGGA TCAGTAGCAA ATCCTCATGG GTCAGGGCAT
    AAGCCACATG GGTCAGAGTA TAACCCTCAT GGGTCAGTAG CGAATCCTCT TGTGTCAGAG
    TATAACACTC ATGGGTCAGT AGCGAATCCT CATGGGTCAG CGCATAACCC TCATGGGTCA
    GTAGCGAATC CTCATGGGTC AGGGCATGAC CCACATGGGT CAGAGTATAA CCCTCATGGG
    TCAGTAGAAA ACCCTCAGGG GTCAGCGCCT AACTCTCATG GATCGGTAGC AAATCCTCAT
    GGGTCAGTAG AAAATCCTCA TGCGTCAGCG CATAACTCTC ATGGATCGGT AGCAAATCCT
    CATGGGTCAG CGCATAACTC TCATGGGTCG GTAGCAAGTC CTCATGGGTC AGCGCATAAC
    TCTCATGGGT CAGGGCATAA TCCTCATGGG TCAGGGCATA ATCCTCATGA GTCAGGGCAT
    AAAACTCATG GGCCAAGGCA TAAGCCTCAT GGGTCAGTAG TGTATCCTCA TGAGCCAATG
    CATAACCCTC ATGGACACCT ACATAATGAA CCAAAAAGTG AGTCACATGG GCCGATACCA
    GAGTCTGCCA GGACAACAGA CGAATCCGTG CTGCCAATCT CAGCATTGGA GTCTGATCCA
    AACATCGAGA TGGTTTCAAT ACACATGTCA CCTGAAGAGA AAAAGGAGGG ATATACTCAT
    AAAGCTCTTA TGAAGTTCTT ATTATCTGGC GGGAAACAGA CTGTACAGCT AAAAGGCATC
    ATATATGACG ACTTTCAAGA TTGTGAATGT AACGGACACT CGAATCGCTG CAGTTACATC
    GACTTCCTGA ACATAGTGAC GTGCGTGAGC TGCAAACACA ACACGAGGGG GCAGAACTGC
    GAACACTGTC GCATGAGCTA CTACAAAAAC ATGTCTGCAG AGCCTGATGA TGAGAACGTG
    TGTATCGAGT GTAACTGTAA TGCGTTGGGC TCAGTTCATG ACCGCTGCAA CGGCACCGGC
    TTCTGTCAGT GTAAAGAAGG CACAACCGGA GTCAAATGTG ATGAATGTCT CCCGGGCTAC
    TATTGGAAAC AGGGCTGTTT ACCGAACGTG TGCGACGACG AGTTACTCTT GTGCCAGAAC
    GGAGGGACGT GCGTTCAGAA TCAGCGCTGC ATCTGCACAC CAGACTTTAA GGGTGTTTTG
    TGCCAACATT CGCGCTGTGA AGAAGGCAAA AAGTGCAACG GCGCCAGCGC TCTCCGTTTG
    AGCCTGGCAC TCCTGCTCTG CCCGCTTCTC GCCACCCTGC TGGGCTCCAC CGCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208498.1
    CDS60..4559
    Translation

    Target ORF information:

    RefSeq Version XM_017353009.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio netrin g2b (ntng2b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353009.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGACGGCA ACTTACAGTG TCAGTGTGAA CACAACACCA CCGGCCAGGA CTGCCAGCGC 
    TGCAAAAAAG GATATAAAGC CAAGACCTGG AAACCAGGAT CGTATTTACC AACTCCGAAC
    GGATCCCCAA ACACATGTTC TCAGGCATTG GCTGGTTCTT CTTCTGGATC CACACCACCA
    GTGGAGGATT CTGGGGAAGT TGAAGAGATT TCTGAAAAGA AAGAGATGAC TTCAGGGAGA
    CATGAAGTAT CTGAAGATGT AAAGAGAGTG GAGACTTTTC TGGAACATAA CAGACACAGG
    GAAGAAACTG CACCTGAACC TGTTGGATCA GTGCGGGAGT CTGCCGAGCC AAAGCCAGAG
    TCGATTCCAA AGCCAATTTT GGAAACACAC AAGCCACTAC ACAAACCTCA AGTTCATGTG
    TCTACAGCGG AATCTCATGG AGCAGCACAA ACATCACATG AATCAGTAAC AAAGCATCAT
    GAACCAATTC AGAAACTTCA TGAGTCAACA GTGAAACCTC ACTCAATGCA TACAACCCAT
    GAGACTATAC AGAAACCTCA TGAACCAAAA GTGAATTCTC ATGAATCAAT GCATAAACCC
    CATGAGCAAA TTGTAAATCT TAATGAATCA ATGTATAAGC CTCATCAGCC AATATGGGAT
    CCACATTCAT CGCCGCAAAA ACCTTTTGAG CCAAACGTGA ACCCCCTTGA GTCAGTAGTG
    AATATTCATG AGTCAACGCA TAAATCACAT GAAGCAGTAG TGAATCCTCA GGAATCAATG
    AATAACCCTC ATGGGTCAGT AGTAAACCCT CACAAGTCAG GGCATAACCC TCATGGGTCA
    GTAGTGAACC CTCACGGGTC AGGGCATAAC CCTCATGGGT CAGTAGTCAA CCCTCACGAG
    TCAGGGCATA ACCCTCATGG GTCAGTAGTG AATCCTCACG AGTCAGGGCA TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAAT AGTAAACCCT
    TATGAGTCAG GGCATAACTC TTATGGGTCA GTAGTGAACC CTCACGGGTC AGTAGTGAAC
    CCTCACGGGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ATCCTCACGA GTCAGGGCAT
    AACCCTCATG GGTCAATAGT GAACCCTCAC GGGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTGAAACCTC ACGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAACCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAG
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAACCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAACC CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA CCCTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AACCCTCACG AGTCAGGGCA TAATCCTCAT GGAACAGTAG TGAATCCTCA CGAGTCAGGG
    CATAACCCTC ATGGGTCAGT AGTGAACCCT CACGAGTCAG GGCATAATCC TCATGGAACA
    GTAGTGAATC CTCACGAGTC AGGGCATAAC CCTCATGGGT CAATAGTAAA CCCTCTTGAT
    TCAGGGCACA ACCCTCATGG GTCGGTAGTG AATCCTCATG GCTCAGGGCT TAACCCTCAT
    GGGTCAGTAG TGAATCCTCA CGAGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA GTAGTGAATT CTCACGAGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA TACTCACGAG TCAGGGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCACG AGTCAGGGCA TAACCCTCAT GGGTCAGTAG TGAATATTCA TAGTTCAGAG
    CAGAAATCTC ATGATCCAGT AGGGAATCCG CATGGGTCAG AGCATAACCT TCATGGGTCA
    GAGGTGAATC ATCATGGATC AGAACATAAA TCTCATGAGC CACTAGTAAA CCCCCATGGG
    TCAGAGCATA GTCCTCATAG GTCAGAAGTG GATCCTCATG GGTCAGTAGT GAATCCTCAT
    GGGTCAGGGC ATAAATCTCA TGACCCAGTT ATGAATCCCC ATGATCCAGT AGTAAATCCT
    CATGGGTCAG AGCATAAATC TCACGATGCA GTAGTGAATC ATCATGGGTC AGTGGATAAA
    TCTCATGAAC CAGTAGTGAA TGCTCATGGG TCAGAGCATA AGCCTCATGG GTCAGGTCAT
    AAACCTCATG AGTCAGTAGT GAATCCTCAT GGATTGGAGC ATAAATCTCA TGACCTAGAA
    GTAAATCCTC ATGGTTCAAA GCACAACACA CATGAGCCAA TAGTGAATCC TCATGGGACA
    GAGCATAACC CTCATGGGTC ATCAGCAAAT CCTCATGAGT CAGATCATAA ATCTCATGAG
    CCAGTAGTTA ATCCACATGG GTCAGGGCAC AACCCTCATG GGTCAGGGCA TAACCCTCAC
    AACCCAGTAG TGAATCCTCA TGTGTCAGAG CATAAATCTC ATGACCCAGT AGTACACCCT
    CATGGGTCAG GGCATAACAA ACATGAGCCA ATAGTTAATC CTATTGGGTC AGGGCATAAC
    TCCCATGGGT CAGTAGTGAA TCCTCATGGG TCAGAGCATA ACCCTCATGG GTCAGTAGTG
    AATCCTCATG GGTCAGAGCA TAACCCTCAT GGGTCAGTAG TGAATCCTCA TGGGTCAGAG
    CATAACCCTC ATGGGTCAGT AGTGAATACT CATGGCTCAG GGCATAACTC TCATGGGTCA
    GTAGCAAATC CTCATGGCTC AGAGAATAAC CCACATGGGT CAGAGCATAA CTCTCATGGG
    TCAGTAGCAA ATCCTCATGG GTCAGGGCAT ACATTTCATG GGTCAATAGC AAATCCTCAT
    GTGTCAGCGG AAAACCCTCA TGGGTCAGTA GCGAATCCTC ATGGGTCAGA GCATAACTCT
    CATGGGTCAG TAGCGAATCC TCACGGGTCA GAGCATAACC CTCATGGGTC AGAAGCGAAT
    CCTCATGGGT CAGAGCATAA CTCTCATGGA TCAGTAGCAA ATCCTCATGG GTCAGGGCAT
    AAGCCACATG GGTCAGAGTA TAACCCTCAT GGGTCAGTAG CGAATCCTCT TGTGTCAGAG
    TATAACACTC ATGGGTCAGT AGCGAATCCT CATGGGTCAG CGCATAACCC TCATGGGTCA
    GTAGCGAATC CTCATGGGTC AGGGCATGAC CCACATGGGT CAGAGTATAA CCCTCATGGG
    TCAGTAGAAA ACCCTCAGGG GTCAGCGCCT AACTCTCATG GATCGGTAGC AAATCCTCAT
    GGGTCAGTAG AAAATCCTCA TGCGTCAGCG CATAACTCTC ATGGATCGGT AGCAAATCCT
    CATGGGTCAG CGCATAACTC TCATGGGTCG GTAGCAAGTC CTCATGGGTC AGCGCATAAC
    TCTCATGGGT CAGGGCATAA TCCTCATGGG TCAGGGCATA ATCCTCATGA GTCAGGGCAT
    AAAACTCATG GGCCAAGGCA TAAGCCTCAT GGGTCAGTAG TGTATCCTCA TGAGCCAATG
    CATAACCCTC ATGGACACCT ACATAATGAA CCAAAAAGTG AGTCACATGG GCCGATACCA
    GAGTCTGCCA GGACAACAGA CGAATCCGTG CTGCCAATCT CAGCATTGGA GTCTGATCCA
    AACATCGAGA TGGTTTCAAT ACACATGTCA CCTGAAGAGA AAAAGGAGGG ATATACTCAT
    AAAGCTCTTA TGAAGTTCTT ATTATCTGGC GGGAAACAGA CTGTACAGCT AAAAGGCATC
    ATATATGACG ACTTTCAAGA TTGTGAATGT AACGGACACT CGAATCGCTG CAGTTACATC
    GACTTCCTGA ACATAGTGAC GTGCGTGAGC TGCAAACACA ACACGAGGGG GCAGAACTGC
    GAACACTGTC GCATGAGCTA CTACAAAAAC ATGTCTGCAG AGCCTGATGA TGAGAACGTG
    TGTATCGAGT GTAACTGTAA TGCGTTGGGC TCAGTTCATG ACCGCTGCAA CGGCACCGGC
    TTCTGTCAGT GTAAAGAAGG CACAACCGGA GTCAAATGTG ATGAATGTCT CCCGGGCTAC
    TATTGGAAAC AGGGCTGTTT ACCGAACGTG TGCGACGACG AGTTACTCTT GTGCCAGAAC
    GGAGGGACGT GCGTTCAGAA TCAGCGCTGC ATCTGCACAC CAGACTTTAA GGGTGTTTTG
    TGCCAACATT CGCGCTGTGA AGAAGGCAAA AAGTGCAACG GCGCCAGCGC TCTCCGTTTG
    AGCCTGGCAC TCCTGCTCTG CCCGCTTCTC GCCACCCTGC TGGGCTCCAC CGCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56958
    Clone ID Related Accession (Same CDS sequence) XM_017353010.1
    Accession Version XM_017353010.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product filaggrin-2-like isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCAG GGAGACATGA AGTATCTGAA GATGTAAAGA GAGTGGAGAC TTTTCTGGAA 
    CATAACAGAC ACAGGGAAGA AACTGCACCT GAACCTGTTG GATCAGTGCG GGAGTCTGCC
    GAGCCAAAGC CAGAGTCGAT TCCAAAGCCA ATTTTGGAAA CACACAAGCC ACTACACAAA
    CCTCAAGTTC ATGTGTCTAC AGCGGAATCT CATGGAGCAG CACAAACATC ACATGAATCA
    GTAACAAAGC ATCATGAACC AATTCAGAAA CTTCATGAGT CAACAGTGAA ACCTCACTCA
    ATGCATACAA CCCATGAGAC TATACAGAAA CCTCATGAAC CAAAAGTGAA TTCTCATGAA
    TCAATGCATA AACCCCATGA GCAAATTGTA AATCTTAATG AATCAATGTA TAAGCCTCAT
    CAGCCAATAT GGGATCCACA TTCATCGCCG CAAAAACCTT TTGAGCCAAA CGTGAACCCC
    CTTGAGTCAG TAGTGAATAT TCATGAGTCA ACGCATAAAT CACATGAAGC AGTAGTGAAT
    CCTCAGGAAT CAATGAATAA CCCTCATGGG TCAGTAGTAA ACCCTCACAA GTCAGGGCAT
    AACCCTCATG GGTCAGTAGT GAACCCTCAC GGGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTCAACCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAG TAGTGAATCC TCACGAGTCA
    GGGCATAACC CTCATGGGTC AGTAGTGAAT CCTCACGAGT CAGGGCATAA CCCTCATGGG
    TCAATAGTAA ACCCTTATGA GTCAGGGCAT AACTCTTATG GGTCAGTAGT GAACCCTCAC
    GGGTCAGTAG TGAACCCTCA CGGGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA ATAGTGAACC CTCACGGGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA ACCTCACGGG TCAGTAGTGA ACCCTCACGA GTCAGGGCAT
    AACCCTCATG GAACAGTAGT GAATCCTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAATA
    GTAAACCCTC TTGAGTCAGG GCACAACCCT CATGGGTCGG TAGTGAATCC TCATGGCTCA
    GGGCTTAACC CTCATGGGTC AGTAGTGAAT CCTCACGAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ACCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAGTAGT GAACCCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAACCCTC ACGAGTCAGG GCATAACCCT
    CATGGGTCAG TAGTGAACCC TCACGAGTCA GGGCATAATC CTCATGGAAC AGTAGTGAAT
    CCTCACGAGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ACCCTCACGA GTCAGGGCAT
    AATCCTCATG GAACAGTAGT GAATCCTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAATA
    GTAAACCCTC TTGATTCAGG GCACAACCCT CATGGGTCGG TAGTGAATCC TCATGGCTCA
    GGGCTTAACC CTCATGGGTC AGTAGTGAAT CCTCACGAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAGTAGT GAATTCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAATACTC ACGAGTCAGG GCATAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAT
    ATTCATAGTT CAGAGCAGAA ATCTCATGAT CCAGTAGGGA ATCCGCATGG GTCAGAGCAT
    AACCTTCATG GGTCAGAGGT GAATCATCAT GGATCAGAAC ATAAATCTCA TGAGCCACTA
    GTAAACCCCC ATGGGTCAGA GCATAGTCCT CATAGGTCAG AAGTGGATCC TCATGGGTCA
    GTAGTGAATC CTCATGGGTC AGGGCATAAA TCTCATGACC CAGTTATGAA TCCCCATGAT
    CCAGTAGTAA ATCCTCATGG GTCAGAGCAT AAATCTCACG ATGCAGTAGT GAATCATCAT
    GGGTCAGTGG ATAAATCTCA TGAACCAGTA GTGAATGCTC ATGGGTCAGA GCATAAGCCT
    CATGGGTCAG GTCATAAACC TCATGAGTCA GTAGTGAATC CTCATGGATT GGAGCATAAA
    TCTCATGACC TAGAAGTAAA TCCTCATGGT TCAAAGCACA ACACACATGA GCCAATAGTG
    AATCCTCATG GGACAGAGCA TAACCCTCAT GGGTCATCAG CAAATCCTCA TGAGTCAGAT
    CATAAATCTC ATGAGCCAGT AGTTAATCCA CATGGGTCAG GGCACAACCC TCATGGGTCA
    GGGCATAACC CTCACAACCC AGTAGTGAAT CCTCATGTGT CAGAGCATAA ATCTCATGAC
    CCAGTAGTAC ACCCTCATGG GTCAGGGCAT AACAAACATG AGCCAATAGT TAATCCTATT
    GGGTCAGGGC ATAACTCCCA TGGGTCAGTA GTGAATCCTC ATGGGTCAGA GCATAACCCT
    CATGGGTCAG TAGTGAATCC TCATGGGTCA GAGCATAACC CTCATGGGTC AGTAGTGAAT
    CCTCATGGGT CAGAGCATAA CCCTCATGGG TCAGTAGTGA ATACTCATGG CTCAGGGCAT
    AACTCTCATG GGTCAGTAGC AAATCCTCAT GGCTCAGAGA ATAACCCACA TGGGTCAGAG
    CATAACTCTC ATGGGTCAGT AGCAAATCCT CATGGGTCAG GGCATACATT TCATGGGTCA
    ATAGCAAATC CTCATGTGTC AGCGGAAAAC CCTCATGGGT CAGTAGCGAA TCCTCATGGG
    TCAGAGCATA ACTCTCATGG GTCAGTAGCG AATCCTCACG GGTCAGAGCA TAACCCTCAT
    GGGTCAGAAG CGAATCCTCA TGGGTCAGAG CATAACTCTC ATGGATCAGT AGCAAATCCT
    CATGGGTCAG GGCATAAGCC ACATGGGTCA GAGTATAACC CTCATGGGTC AGTAGCGAAT
    CCTCTTGTGT CAGAGTATAA CACTCATGGG TCAGTAGCGA ATCCTCATGG GTCAGCGCAT
    AACCCTCATG GGTCAGTAGC GAATCCTCAT GGGTCAGGGC ATGACCCACA TGGGTCAGAG
    TATAACCCTC ATGGGTCAGT AGAAAACCCT CAGGGGTCAG CGCCTAACTC TCATGGATCG
    GTAGCAAATC CTCATGGGTC AGTAGAAAAT CCTCATGCGT CAGCGCATAA CTCTCATGGA
    TCGGTAGCAA ATCCTCATGG GTCAGCGCAT AACTCTCATG GGTCGGTAGC AAGTCCTCAT
    GGGTCAGCGC ATAACTCTCA TGGGTCAGGG CATAATCCTC ATGGGTCAGG GCATAATCCT
    CATGAGTCAG GGCATAAAAC TCATGGGCCA AGGCATAAGC CTCATGGGTC AGTAGTGTAT
    CCTCATGAGC CAATGCATAA CCCTCATGGA CACCTACATA ATGAACCAAA AAGTGAGTCA
    CATGGGCCGA TACCAGAGTC TGCCAGGACA ACAGACGAAT CCGTGCTGCC AATCTCAGCA
    TTGGAGTCTG ATCCAAACAT CGAGATGGTT TCAATACACA TGTCACCTGA AGAGAAAAAG
    GAGGGATATA CTCATAAAGC TCTTATGAAG TTCTTATTAT CTGGCGGGAA ACAGACTGTA
    CAGCTAAAAG GCATCATATA TGACGACTTT CAAGATTGTG AATGTAACGG ACACTCGAAT
    CGCTGCAGTT ACATCGACTT CCTGAACATA GTGACGTGCG TGAGCTGCAA ACACAACACG
    AGGGGGCAGA ACTGCGAACA CTGTCGCATG AGCTACTACA AAAACATGTC TGCAGAGCCT
    GATGATGAGA ACGTGTGTAT CGAGTGTAAC TGTAATGCGT TGGGCTCAGT TCATGACCGC
    TGCAACGGCA CCGGCTTCTG TCAGTGTAAA GAAGGCACAA CCGGAGTCAA ATGTGATGAA
    TGTCTCCCGG GCTACTATTG GAAACAGGGC TGTTTACCGA ACGTGTGCGA CGACGAGTTA
    CTCTTGTGCC AGAACGGAGG GACGTGCGTT CAGAATCAGC GCTGCATCTG CACACCAGAC
    TTTAAGGGTG TTTTGTGCCA ACATTCGCGC TGTGAAGAAG GCAAAAAGTG CAACGGCGCC
    AGCGCTCTCC GTTTGAGCCT GGCACTCCTG CTCTGCCCGC TTCTCGCCAC CCTGCTGGGC
    TCCACCGCCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208499.1
    CDS185..4459
    Translation

    Target ORF information:

    RefSeq Version XM_017353010.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio netrin g2b (ntng2b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCAG GGAGACATGA AGTATCTGAA GATGTAAAGA GAGTGGAGAC TTTTCTGGAA 
    CATAACAGAC ACAGGGAAGA AACTGCACCT GAACCTGTTG GATCAGTGCG GGAGTCTGCC
    GAGCCAAAGC CAGAGTCGAT TCCAAAGCCA ATTTTGGAAA CACACAAGCC ACTACACAAA
    CCTCAAGTTC ATGTGTCTAC AGCGGAATCT CATGGAGCAG CACAAACATC ACATGAATCA
    GTAACAAAGC ATCATGAACC AATTCAGAAA CTTCATGAGT CAACAGTGAA ACCTCACTCA
    ATGCATACAA CCCATGAGAC TATACAGAAA CCTCATGAAC CAAAAGTGAA TTCTCATGAA
    TCAATGCATA AACCCCATGA GCAAATTGTA AATCTTAATG AATCAATGTA TAAGCCTCAT
    CAGCCAATAT GGGATCCACA TTCATCGCCG CAAAAACCTT TTGAGCCAAA CGTGAACCCC
    CTTGAGTCAG TAGTGAATAT TCATGAGTCA ACGCATAAAT CACATGAAGC AGTAGTGAAT
    CCTCAGGAAT CAATGAATAA CCCTCATGGG TCAGTAGTAA ACCCTCACAA GTCAGGGCAT
    AACCCTCATG GGTCAGTAGT GAACCCTCAC GGGTCAGGGC ATAACCCTCA TGGGTCAGTA
    GTCAACCCTC ACGAGTCAGG GCATAACCCT CATGGGTCAG TAGTGAATCC TCACGAGTCA
    GGGCATAACC CTCATGGGTC AGTAGTGAAT CCTCACGAGT CAGGGCATAA CCCTCATGGG
    TCAATAGTAA ACCCTTATGA GTCAGGGCAT AACTCTTATG GGTCAGTAGT GAACCCTCAC
    GGGTCAGTAG TGAACCCTCA CGGGTCAGGG CATAACCCTC ATGGGTCAGT AGTGAATCCT
    CACGAGTCAG GGCATAACCC TCATGGGTCA ATAGTGAACC CTCACGGGTC AGGGCATAAC
    CCTCATGGGT CAGTAGTGAA ACCTCACGGG TCAGTAGTGA ACCCTCACGA GTCAGGGCAT
    AACCCTCATG GAACAGTAGT GAATCCTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAATA
    GTAAACCCTC TTGAGTCAGG GCACAACCCT CATGGGTCGG TAGTGAATCC TCATGGCTCA
    GGGCTTAACC CTCATGGGTC AGTAGTGAAT CCTCACGAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ACCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAGTAGT GAACCCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAACCCTC ACGAGTCAGG GCATAACCCT
    CATGGGTCAG TAGTGAACCC TCACGAGTCA GGGCATAATC CTCATGGAAC AGTAGTGAAT
    CCTCACGAGT CAGGGCATAA CCCTCATGGG TCAGTAGTGA ACCCTCACGA GTCAGGGCAT
    AATCCTCATG GAACAGTAGT GAATCCTCAC GAGTCAGGGC ATAACCCTCA TGGGTCAATA
    GTAAACCCTC TTGATTCAGG GCACAACCCT CATGGGTCGG TAGTGAATCC TCATGGCTCA
    GGGCTTAACC CTCATGGGTC AGTAGTGAAT CCTCACGAGT CAGGGCATAA CCCTCATGGG
    TCAGTAGTGA ATCCTCACGA GTCAGGGCAT AACCCTCATG GGTCAGTAGT GAATTCTCAC
    GAGTCAGGGC ATAACCCTCA TGGGTCAGTA GTGAATACTC ACGAGTCAGG GCATAACCCT
    CATGGGTCAG TAGTGAATCC TCACGAGTCA GGGCATAACC CTCATGGGTC AGTAGTGAAT
    ATTCATAGTT CAGAGCAGAA ATCTCATGAT CCAGTAGGGA ATCCGCATGG GTCAGAGCAT
    AACCTTCATG GGTCAGAGGT GAATCATCAT GGATCAGAAC ATAAATCTCA TGAGCCACTA
    GTAAACCCCC ATGGGTCAGA GCATAGTCCT CATAGGTCAG AAGTGGATCC TCATGGGTCA
    GTAGTGAATC CTCATGGGTC AGGGCATAAA TCTCATGACC CAGTTATGAA TCCCCATGAT
    CCAGTAGTAA ATCCTCATGG GTCAGAGCAT AAATCTCACG ATGCAGTAGT GAATCATCAT
    GGGTCAGTGG ATAAATCTCA TGAACCAGTA GTGAATGCTC ATGGGTCAGA GCATAAGCCT
    CATGGGTCAG GTCATAAACC TCATGAGTCA GTAGTGAATC CTCATGGATT GGAGCATAAA
    TCTCATGACC TAGAAGTAAA TCCTCATGGT TCAAAGCACA ACACACATGA GCCAATAGTG
    AATCCTCATG GGACAGAGCA TAACCCTCAT GGGTCATCAG CAAATCCTCA TGAGTCAGAT
    CATAAATCTC ATGAGCCAGT AGTTAATCCA CATGGGTCAG GGCACAACCC TCATGGGTCA
    GGGCATAACC CTCACAACCC AGTAGTGAAT CCTCATGTGT CAGAGCATAA ATCTCATGAC
    CCAGTAGTAC ACCCTCATGG GTCAGGGCAT AACAAACATG AGCCAATAGT TAATCCTATT
    GGGTCAGGGC ATAACTCCCA TGGGTCAGTA GTGAATCCTC ATGGGTCAGA GCATAACCCT
    CATGGGTCAG TAGTGAATCC TCATGGGTCA GAGCATAACC CTCATGGGTC AGTAGTGAAT
    CCTCATGGGT CAGAGCATAA CCCTCATGGG TCAGTAGTGA ATACTCATGG CTCAGGGCAT
    AACTCTCATG GGTCAGTAGC AAATCCTCAT GGCTCAGAGA ATAACCCACA TGGGTCAGAG
    CATAACTCTC ATGGGTCAGT AGCAAATCCT CATGGGTCAG GGCATACATT TCATGGGTCA
    ATAGCAAATC CTCATGTGTC AGCGGAAAAC CCTCATGGGT CAGTAGCGAA TCCTCATGGG
    TCAGAGCATA ACTCTCATGG GTCAGTAGCG AATCCTCACG GGTCAGAGCA TAACCCTCAT
    GGGTCAGAAG CGAATCCTCA TGGGTCAGAG CATAACTCTC ATGGATCAGT AGCAAATCCT
    CATGGGTCAG GGCATAAGCC ACATGGGTCA GAGTATAACC CTCATGGGTC AGTAGCGAAT
    CCTCTTGTGT CAGAGTATAA CACTCATGGG TCAGTAGCGA ATCCTCATGG GTCAGCGCAT
    AACCCTCATG GGTCAGTAGC GAATCCTCAT GGGTCAGGGC ATGACCCACA TGGGTCAGAG
    TATAACCCTC ATGGGTCAGT AGAAAACCCT CAGGGGTCAG CGCCTAACTC TCATGGATCG
    GTAGCAAATC CTCATGGGTC AGTAGAAAAT CCTCATGCGT CAGCGCATAA CTCTCATGGA
    TCGGTAGCAA ATCCTCATGG GTCAGCGCAT AACTCTCATG GGTCGGTAGC AAGTCCTCAT
    GGGTCAGCGC ATAACTCTCA TGGGTCAGGG CATAATCCTC ATGGGTCAGG GCATAATCCT
    CATGAGTCAG GGCATAAAAC TCATGGGCCA AGGCATAAGC CTCATGGGTC AGTAGTGTAT
    CCTCATGAGC CAATGCATAA CCCTCATGGA CACCTACATA ATGAACCAAA AAGTGAGTCA
    CATGGGCCGA TACCAGAGTC TGCCAGGACA ACAGACGAAT CCGTGCTGCC AATCTCAGCA
    TTGGAGTCTG ATCCAAACAT CGAGATGGTT TCAATACACA TGTCACCTGA AGAGAAAAAG
    GAGGGATATA CTCATAAAGC TCTTATGAAG TTCTTATTAT CTGGCGGGAA ACAGACTGTA
    CAGCTAAAAG GCATCATATA TGACGACTTT CAAGATTGTG AATGTAACGG ACACTCGAAT
    CGCTGCAGTT ACATCGACTT CCTGAACATA GTGACGTGCG TGAGCTGCAA ACACAACACG
    AGGGGGCAGA ACTGCGAACA CTGTCGCATG AGCTACTACA AAAACATGTC TGCAGAGCCT
    GATGATGAGA ACGTGTGTAT CGAGTGTAAC TGTAATGCGT TGGGCTCAGT TCATGACCGC
    TGCAACGGCA CCGGCTTCTG TCAGTGTAAA GAAGGCACAA CCGGAGTCAA ATGTGATGAA
    TGTCTCCCGG GCTACTATTG GAAACAGGGC TGTTTACCGA ACGTGTGCGA CGACGAGTTA
    CTCTTGTGCC AGAACGGAGG GACGTGCGTT CAGAATCAGC GCTGCATCTG CACACCAGAC
    TTTAAGGGTG TTTTGTGCCA ACATTCGCGC TGTGAAGAAG GCAAAAAGTG CAACGGCGCC
    AGCGCTCTCC GTTTGAGCCT GGCACTCCTG CTCTGCCCGC TTCTCGCCAC CCTGCTGGGC
    TCCACCGCCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.