HECW2A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW2A
Entrez Gene ID	100150269
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a
Synonyms	si:dkey-191g15.6
General protein information	Preferred Names HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a Names E3 ubiquitin-protein ligase HECW2-like hecw2 nedl2a
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009304701.3	XP_009302976.1	E3 ubiquitin-protein ligase HECW2-like isoform X1
XM_009304701.2	XP_009302976.1	E3 ubiquitin-protein ligase HECW2-like isoform X1
XM_021478948.1	XP_021334623.1	E3 ubiquitin-protein ligase HECW2-like isoform X1
XM_021478949.1	XP_021334624.1	E3 ubiquitin-protein ligase HECW2-like isoform X2
XM_009304702.3	XP_009302977.1	E3 ubiquitin-protein ligase HECW2-like isoform X2
XM_009304702.2	XP_009302977.1	E3 ubiquitin-protein ligase HECW2-like isoform X2

Pathways from BioSystems
Gene Ontology

The following HECW2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa48865	XM_009304701.3 Latest version!	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa48865	XM_021478948.1 Latest version!	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa48866	XM_009304702.3 Latest version!	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa48866	XM_021478949.1 Latest version!	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa48865	XM_009304701.2	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa48866	XM_009304702.2	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW2A ( XM_009304701.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302976.1 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48865
Clone ID Related Accession (Same CDS sequence)	XM_009304701.3 , XM_009304701.2 , XM_021478948.1
Accession Version	XM_009304701.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase HECW2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394753.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304701.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCTC AGGGGCGGGA TCACCTGGCC ACACGGGAGT CTGCAGGCCG AAGACGCAGC 
CCGAATGTGC GGCACACGCT CAGCCCGGAA AACCTGCGAA GCCTGGCGGA GCGCGGTGGA 
GCTGAACAAG CGGGAGTAGG ACTGCTCAGA GCCAATAGCG ACACAGACCT GGTCAGTTCT 
CAAGGGCGAT CCTCACTCAC AGCCTCCACT CTAGAGTTTA CACTGTGCCG CGGACAGAAC 
CTCGTCATCT CATGGGACAT CAAGGAGGAG GTGGACGCCA CTGACTGGAT CGGCCTGTAC 
CACATAGATG AAACCTGTCC ATCAAATGTG TGGGACTGTA AGAACCGTGG GGTAAACGGC 
ACACAGTGTG GTCAGATCGT CTGGAGATTA GAGGTCGGGC CATACTTTCT TGAATCTGAG 
ACAAGGATCT GCTTTAAATA CTACCATGGA GTAAGTGGAG CATTGAGAGC CACGACTCCC 
TGCATCACAG TCAAGAACCC CAGAGCCTCG GTTGATGGCC AAGGAGAAGG ACAACAAGCT 
TCAGACAGTA GTCGAAAACT GGTTAGCTTT ACGCTATCAG ATATCCGAGC TGTTGGCCTG 
AAGAAGGGGA TGTTTTTTAA CCCAGACCCT TATCTGAAGA TGAGCATTCA TCCAGGCAAA 
AGAAGCTGCT TTCCGACCTT TTCCCATCAC GGCCAAGAGA GGCGATCCGG CATTACAACT 
AACACCACTA ATCCTGTTTG GCATGGAGAG AAACATACTT TTGTGGCTCT TATGACAGAT 
GTATTAGTGA TTGAGGTTAA AGACAAGTTT GCTAAAAGTC GACCCATTAT TAAACGCTTC 
TTGGGTCAGC TGAATATTCC TGTGCAGCTG CTGCTGGAGA GACATTCGAC AGGGAACCAG 
TCACTCAGCT TCTCCCTATG TCGTCGTTTG CCCACTGATC ATGTGAGTGG TCAAATGCAC 
TTTAAAGTGG ATGTCACGTC TATGGGCCCA GATGATGTGT CTCCCGAGAC AATTCTGGGG 
GCAGCAGCCC TCAATGGAGC TCCTGGAACC CCTTCAGATG ACGAAGAGCT GCCTCATCCT 
CTACCCATCT CATCAGCAGG CCCTTCCCCA ACAGCCTCCC TGGGGTCCAA TAGAAATGGG 
GAGGGGAGCA GCATTCCCTC CCCCGACACA GAAATCTATG GTGGCACCCT GGACGACGAG 
GCCCCTTCTT CTCCAGACCT GCTCCAGGGG TCTTTCAGCG AGCAGCTTGA TGCCATCGAG 
GCTCCTAAGG GCCCAGGTGA CAGACCTCTA GGAGCAGCTT CTCCAAAACT CCGCTCCAGC 
TTTCCCACAC ATACGCGACT CAGTGCCATG CTGCACATCG ACTCAGATGA AGATGAGGAC 
AGATCTGCTG CTACAGAGGC CACGTTGTCA ACATTTAACG GCATTCCAAG GTCTTATCAA 
ACACCCCCAA AAGCAGCAGC AAGAGGAGCT GTGTTTGATG GAGGCATAGA GGAACCTTCA 
GAAGAGGCTG GGCTGGAGTC TGAAGTAGAA ACTCTGAATG CAGGAGAAGA GGTGCCTCCT 
GAGCCAGAGG TCGTGCCCAG TTCAGTCGCT CCCGAGGTGG AAACCGCCAG TCTGATGGAG 
AGGCAGGAGG AACAGGGCCG GGAGGTGGAG GTAGAGGAGG AGGAGGAAGA GGGCCTGGCG 
CGCAGTGAAG AGTTGGCAAC CGAAGTTGAT ATCTCCTCAA TGGCATCTGA CTGCTGCCCG 
GGGCCTGCAT CAGCATCTCA GGAGGCGGAG CAGGGGGCAG AGGCAGCCAT TGACCCAGGG 
GGAGAAGATC TCTCAGATGA TCCACCTACC ACTTACGAGG TTGCAGAGAC TGCTGAAGAA 
ACACATCTAA CTAATGAGCC GGAGAGAGAG GCTCCAAAAC CAGAGCCCCC CACCAACGAG 
GAAGAGGAAT GTGAAGATGT GACTGAGGAT ACCATGTCCA GAAGATGGAG CCTGGAGGCC 
ACAGCAGGAG TGTCACAGGA GGATGAAGAT GAGTTGATAC CATCCGGAGA TGGGGAATCT 
GTGGACTCTG AGACTTTTGT TACGGAGACA GAGTCAGAAT TAGGCGTTCC TCAGGTAAAC 
GGAGATCAGC GTGTTAATTC TCTGCCATCT GTAAGACAAG ACATCCACCG ATACCAGCGT 
GTGGACGAGC CCTTACCTCC CAACTGGGAG GCTCGTATTG ACAGTCATGG TCGTATCTTC 
TTTGTGGATC ACGTGAATCG CACCACCACA TGGCAGAGAC CCACTGGCCC TCCGGCTCCA 
CAGGGTCTGA CCCGCTCCAG CTCCATTCTG CAGATGGAAC AACTCAACCG CAGGTATCAG 
AGTATTCGCA GGACAATGAC CAGTGAAAGG TCTGAGGAAC CCAGAGTTGA AGATCCTCCA 
GCTGATGAAA CAGACGTGCT GGCTCAGTCC ATCAGTGAAT ACCGGAGGGA TGGCATTATT 
GGTCAGTCCA GCTCACGCTC TCGTCTGGCT CTTCTGCTTC AGTCTCCTGG TGCAAAGTTC 
CTGTCCAGTC CAGACTTCTT CTCTGTTCTG CATTCTAATC CTAGTGCCTA TCGGATGTTC 
ACAAGTAACA CGTGTCTGAA GCACATGATC AGTAAAGTTC GACGGGATGC CCAACACTTT 
GAGCGGTACC AGCACAACCG AGACCTGGTC AACTTCCTCA ATCTGTTCTC CAACAAGCAG 
CTGGAGCTTC CCCGGGGCTG GGAGATGAAA CATGACCATA CTGGGAAGGC CTTCTTTGTA 
GACCACAACT GTCGTGCCAC AACATTTATT GACCCGCGGC TGCCCTTGCA GAACTCGCGG 
TCCAGCGGAT TGTTGGCTCA CAGACAGCAC CTGTCCCGCC AGCGCAGCCA CAGTGCAGGA 
GAGGTTGGAG ATGACTCTCG TTCCCCGAGT CCTCCAGTCC AGGGCCGGTC ATCTAGAAAC 
AGTCAGTATC AGGACCTGGT GCCTGTGGCC TATAATGATA AGATTGTTGC CTTTTTGCGA 
CAACCCAACA TTTTTGAGAT CCTGCAAGAG AGACAGCCAG AACTTGTGAG AAACCATTCC 
CTTAGGGAGA AGGTTCAGTT TATCCGCAAT GAGGGGGCAA CAGGGTTAAC TCGTCTGTCC 
AGTGATGCAG ACCTGGTTAT GTTGCTCAGC CTGTTTGAAG AGGAAGTGAT GTCGTATGTT 
CCGCCCAATG CCTTACTGCT CTCCAGCTAC TGTCATGCGT CCCCACAGAG CTCCCCTGGA 
ACCCCACGGG CTAATGCGCG AGCCCCTGCT CCCTACAAGC GGGATTTTGA GGCCAAATTA 
AGAAACTTCT ACCGCAAAAT GGAGACCAAG GGCTACGGAC AGGGACCAGG AAAAGTGAAG 
TTAATAATAA GAAGAGATCA CCTGCTTGAG GACGCTTTCA ATCAGATCAT GTGTTATTCC 
CGCAAAGACC TTCAAAGGAG CAGGCTTTAC GTCAGCTTTG TTGGAGAAGA AGGATTGGAC 
TATAGTGGTC CATCCAGGGA GTTCTTCTTC CTGGTTTCCC GTGAGCTTTT TAACCCATAC 
TATGGACTCT TTGAATACTC AGCTAATGAC ACTTACACTG TGCAGATCAG CCCTATGTCA 
GCCTTTGTGG ACAACCATCA TGAGTGGTTC CGGTTCAGTG GCCGCATTCT GGGTCTTGCT 
CTAATTCATC AGTACCTGCT GGATGCCTTC TTCACCCGAC CATTTTACAA AGGCCTTCTC 
CGCATTCCAT GTGATCTGAG TGATCTAGAG TTTCTGGATG AAGAGTTTCA TCAGAGTTTG 
CAGTGGATGA AGGATAATGA CATCGAGGAC ATGCTGGACC TCACCTTTAC AGTCAATGAG 
GAGGTGTTTG GACAGATAAC AGAACGTGAG CTAAAGCCGG GTGGATCTGG CATCGCTGTG 
TCAGACAAGA ACAAGAAAGA GTACATTGAG CGCATGGTGA AGTGGCGGAT TGAGAGAGGT 
GTGGCTCAGC AGACAGAGAG TCTTGTGCGA GGATTTTATG AGGTGGTGGA TGTGCGGCTC 
GTCTCAGTGT TTGATGCCCG AGAGCTGGAG CTGGTCATTG CTGGAACAGC AGAGATAGAC 
CTGTCCGACT GGAGAAACAA CACAGAATAC AGGGGAGGTT ATCATGACAA CCACATAGTG 
ATTCGATGGT TCTGGGCTGC TGTGGAGAGA TTCAACAATG AACAGAGACT ACGACTCCTT 
CAGTTTGTAA CAGGAACTTC CAGCATTCCC TATGAGGGCT TTGCCTCTCT GCGGGGCAGT 
AATGGTCCAC GCCGATTCTG TGTGGAGAAA TGGGGCAAAG TGACCTCCCT CCCACGGGCT 
CACACCTGCT TTAACCGACT GGATCTCCCT CCCTACCCCT CGTTCTCCAT GCTCTATGAG 
AAGATGGTCA CGGCTGTGGA GGAAACCAGC ACCTTTGGTC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302976.1
CDS	1071..5498
Translation	MAPQGRDHLATRESAGRRRSPNVRHTLSPENLRSLAERGGAEQAGVGLLRANSDTDLVSSQGRSSLTASTLEFTLCRGQNLVISWDIKEEVDATDWIGLYHIDETCPSNVWDCKNRGVNGTQCGQIVWRLEVGPYFLESETRICFKYYHGVSGALRATTPCITVKNPRASVDGQGEGQQASDSSRKLVSFTLSDIRAVGLKKGMFFNPDPYLKMSIHPGKRSCFPTFSHHGQERRSGITTNTTNPVWHGEKHTFVALMTDVLVIEVKDKFAKSRPIIKRFLGQLNIPVQLLLERHSTGNQSLSFSLCRRLPTDHVSGQMHFKVDVTSMGPDDVSPETILGAAALNGAPGTPSDDEELPHPLPISSAGPSPTASLGSNRNGEGSSIPSPDTEIYGGTLDDEAPSSPDLLQGSFSEQLDAIEAPKGPGDRPLGAASPKLRSSFPTHTRLSAMLHIDSDEDEDRSAATEATLSTFNGIPRSYQTPPKAAARGAVFDGGIEEPSEEAGLESEVETLNAGEEVPPEPEVVPSSVAPEVETASLMERQEEQGREVEVEEEEEEGLARSEELATEVDISSMASDCCPGPASASQEAEQGAEAAIDPGGEDLSDDPPTTYEVAETAEETHLTNEPEREAPKPEPPTNEEEECEDVTEDTMSRRWSLEATAGVSQEDEDELIPSGDGESVDSETFVTETESELGVPQVNGDQRVNSLPSVRQDIHRYQRVDEPLPPNWEARIDSHGRIFFVDHVNRTTTWQRPTGPPAPQGLTRSSSILQMEQLNRRYQSIRRTMTSERSEEPRVEDPPADETDVLAQSISEYRRDGIIGQSSSRSRLALLLQSPGAKFLSSPDFFSVLHSNPSAYRMFTSNTCLKHMISKVRRDAQHFERYQHNRDLVNFLNLFSNKQLELPRGWEMKHDHTGKAFFVDHNCRATTFIDPRLPLQNSRSSGLLAHRQHLSRQRSHSAGEVGDDSRSPSPPVQGRSSRNSQYQDLVPVAYNDKIVAFLRQPNIFEILQERQPELVRNHSLREKVQFIRNEGATGLTRLSSDADLVMLLSLFEEEVMSYVPPNALLLSSYCHASPQSSPGTPRANARAPAPYKRDFEAKLRNFYRKMETKGYGQGPGKVKLIIRRDHLLEDAFNQIMCYSRKDLQRSRLYVSFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLRIPCDLSDLEFLDEEFHQSLQWMKDNDIEDMLDLTFTVNEEVFGQITERELKPGGSGIAVSDKNKKEYIERMVKWRIERGVAQQTESLVRGFYEVVDVRLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKVTSLPRAHTCFNRLDLPPYPSFSMLYEKMVTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009304701.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304701.3

ORF Insert Sequence:

ATGGCGCCTC AGGGGCGGGA TCACCTGGCC ACACGGGAGT CTGCAGGCCG AAGACGCAGC 
CCGAATGTGC GGCACACGCT CAGCCCGGAA AACCTGCGAA GCCTGGCGGA GCGCGGTGGA 
GCTGAACAAG CGGGAGTAGG ACTGCTCAGA GCCAATAGCG ACACAGACCT GGTCAGTTCT 
CAAGGGCGAT CCTCACTCAC AGCCTCCACT CTAGAGTTTA CACTGTGCCG CGGACAGAAC 
CTCGTCATCT CATGGGACAT CAAGGAGGAG GTGGACGCCA CTGACTGGAT CGGCCTGTAC 
CACATAGATG AAACCTGTCC ATCAAATGTG TGGGACTGTA AGAACCGTGG GGTAAACGGC 
ACACAGTGTG GTCAGATCGT CTGGAGATTA GAGGTCGGGC CATACTTTCT TGAATCTGAG 
ACAAGGATCT GCTTTAAATA CTACCATGGA GTAAGTGGAG CATTGAGAGC CACGACTCCC 
TGCATCACAG TCAAGAACCC CAGAGCCTCG GTTGATGGCC AAGGAGAAGG ACAACAAGCT 
TCAGACAGTA GTCGAAAACT GGTTAGCTTT ACGCTATCAG ATATCCGAGC TGTTGGCCTG 
AAGAAGGGGA TGTTTTTTAA CCCAGACCCT TATCTGAAGA TGAGCATTCA TCCAGGCAAA 
AGAAGCTGCT TTCCGACCTT TTCCCATCAC GGCCAAGAGA GGCGATCCGG CATTACAACT 
AACACCACTA ATCCTGTTTG GCATGGAGAG AAACATACTT TTGTGGCTCT TATGACAGAT 
GTATTAGTGA TTGAGGTTAA AGACAAGTTT GCTAAAAGTC GACCCATTAT TAAACGCTTC 
TTGGGTCAGC TGAATATTCC TGTGCAGCTG CTGCTGGAGA GACATTCGAC AGGGAACCAG 
TCACTCAGCT TCTCCCTATG TCGTCGTTTG CCCACTGATC ATGTGAGTGG TCAAATGCAC 
TTTAAAGTGG ATGTCACGTC TATGGGCCCA GATGATGTGT CTCCCGAGAC AATTCTGGGG 
GCAGCAGCCC TCAATGGAGC TCCTGGAACC CCTTCAGATG ACGAAGAGCT GCCTCATCCT 
CTACCCATCT CATCAGCAGG CCCTTCCCCA ACAGCCTCCC TGGGGTCCAA TAGAAATGGG 
GAGGGGAGCA GCATTCCCTC CCCCGACACA GAAATCTATG GTGGCACCCT GGACGACGAG 
GCCCCTTCTT CTCCAGACCT GCTCCAGGGG TCTTTCAGCG AGCAGCTTGA TGCCATCGAG 
GCTCCTAAGG GCCCAGGTGA CAGACCTCTA GGAGCAGCTT CTCCAAAACT CCGCTCCAGC 
TTTCCCACAC ATACGCGACT CAGTGCCATG CTGCACATCG ACTCAGATGA AGATGAGGAC 
AGATCTGCTG CTACAGAGGC CACGTTGTCA ACATTTAACG GCATTCCAAG GTCTTATCAA 
ACACCCCCAA AAGCAGCAGC AAGAGGAGCT GTGTTTGATG GAGGCATAGA GGAACCTTCA 
GAAGAGGCTG GGCTGGAGTC TGAAGTAGAA ACTCTGAATG CAGGAGAAGA GGTGCCTCCT 
GAGCCAGAGG TCGTGCCCAG TTCAGTCGCT CCCGAGGTGG AAACCGCCAG TCTGATGGAG 
AGGCAGGAGG AACAGGGCCG GGAGGTGGAG GTAGAGGAGG AGGAGGAAGA GGGCCTGGCG 
CGCAGTGAAG AGTTGGCAAC CGAAGTTGAT ATCTCCTCAA TGGCATCTGA CTGCTGCCCG 
GGGCCTGCAT CAGCATCTCA GGAGGCGGAG CAGGGGGCAG AGGCAGCCAT TGACCCAGGG 
GGAGAAGATC TCTCAGATGA TCCACCTACC ACTTACGAGG TTGCAGAGAC TGCTGAAGAA 
ACACATCTAA CTAATGAGCC GGAGAGAGAG GCTCCAAAAC CAGAGCCCCC CACCAACGAG 
GAAGAGGAAT GTGAAGATGT GACTGAGGAT ACCATGTCCA GAAGATGGAG CCTGGAGGCC 
ACAGCAGGAG TGTCACAGGA GGATGAAGAT GAGTTGATAC CATCCGGAGA TGGGGAATCT 
GTGGACTCTG AGACTTTTGT TACGGAGACA GAGTCAGAAT TAGGCGTTCC TCAGGTAAAC 
GGAGATCAGC GTGTTAATTC TCTGCCATCT GTAAGACAAG ACATCCACCG ATACCAGCGT 
GTGGACGAGC CCTTACCTCC CAACTGGGAG GCTCGTATTG ACAGTCATGG TCGTATCTTC 
TTTGTGGATC ACGTGAATCG CACCACCACA TGGCAGAGAC CCACTGGCCC TCCGGCTCCA 
CAGGGTCTGA CCCGCTCCAG CTCCATTCTG CAGATGGAAC AACTCAACCG CAGGTATCAG 
AGTATTCGCA GGACAATGAC CAGTGAAAGG TCTGAGGAAC CCAGAGTTGA AGATCCTCCA 
GCTGATGAAA CAGACGTGCT GGCTCAGTCC ATCAGTGAAT ACCGGAGGGA TGGCATTATT 
GGTCAGTCCA GCTCACGCTC TCGTCTGGCT CTTCTGCTTC AGTCTCCTGG TGCAAAGTTC 
CTGTCCAGTC CAGACTTCTT CTCTGTTCTG CATTCTAATC CTAGTGCCTA TCGGATGTTC 
ACAAGTAACA CGTGTCTGAA GCACATGATC AGTAAAGTTC GACGGGATGC CCAACACTTT 
GAGCGGTACC AGCACAACCG AGACCTGGTC AACTTCCTCA ATCTGTTCTC CAACAAGCAG 
CTGGAGCTTC CCCGGGGCTG GGAGATGAAA CATGACCATA CTGGGAAGGC CTTCTTTGTA 
GACCACAACT GTCGTGCCAC AACATTTATT GACCCGCGGC TGCCCTTGCA GAACTCGCGG 
TCCAGCGGAT TGTTGGCTCA CAGACAGCAC CTGTCCCGCC AGCGCAGCCA CAGTGCAGGA 
GAGGTTGGAG ATGACTCTCG TTCCCCGAGT CCTCCAGTCC AGGGCCGGTC ATCTAGAAAC 
AGTCAGTATC AGGACCTGGT GCCTGTGGCC TATAATGATA AGATTGTTGC CTTTTTGCGA 
CAACCCAACA TTTTTGAGAT CCTGCAAGAG AGACAGCCAG AACTTGTGAG AAACCATTCC 
CTTAGGGAGA AGGTTCAGTT TATCCGCAAT GAGGGGGCAA CAGGGTTAAC TCGTCTGTCC 
AGTGATGCAG ACCTGGTTAT GTTGCTCAGC CTGTTTGAAG AGGAAGTGAT GTCGTATGTT 
CCGCCCAATG CCTTACTGCT CTCCAGCTAC TGTCATGCGT CCCCACAGAG CTCCCCTGGA 
ACCCCACGGG CTAATGCGCG AGCCCCTGCT CCCTACAAGC GGGATTTTGA GGCCAAATTA 
AGAAACTTCT ACCGCAAAAT GGAGACCAAG GGCTACGGAC AGGGACCAGG AAAAGTGAAG 
TTAATAATAA GAAGAGATCA CCTGCTTGAG GACGCTTTCA ATCAGATCAT GTGTTATTCC 
CGCAAAGACC TTCAAAGGAG CAGGCTTTAC GTCAGCTTTG TTGGAGAAGA AGGATTGGAC 
TATAGTGGTC CATCCAGGGA GTTCTTCTTC CTGGTTTCCC GTGAGCTTTT TAACCCATAC 
TATGGACTCT TTGAATACTC AGCTAATGAC ACTTACACTG TGCAGATCAG CCCTATGTCA 
GCCTTTGTGG ACAACCATCA TGAGTGGTTC CGGTTCAGTG GCCGCATTCT GGGTCTTGCT 
CTAATTCATC AGTACCTGCT GGATGCCTTC TTCACCCGAC CATTTTACAA AGGCCTTCTC 
CGCATTCCAT GTGATCTGAG TGATCTAGAG TTTCTGGATG AAGAGTTTCA TCAGAGTTTG 
CAGTGGATGA AGGATAATGA CATCGAGGAC ATGCTGGACC TCACCTTTAC AGTCAATGAG 
GAGGTGTTTG GACAGATAAC AGAACGTGAG CTAAAGCCGG GTGGATCTGG CATCGCTGTG 
TCAGACAAGA ACAAGAAAGA GTACATTGAG CGCATGGTGA AGTGGCGGAT TGAGAGAGGT 
GTGGCTCAGC AGACAGAGAG TCTTGTGCGA GGATTTTATG AGGTGGTGGA TGTGCGGCTC 
GTCTCAGTGT TTGATGCCCG AGAGCTGGAG CTGGTCATTG CTGGAACAGC AGAGATAGAC 
CTGTCCGACT GGAGAAACAA CACAGAATAC AGGGGAGGTT ATCATGACAA CCACATAGTG 
ATTCGATGGT TCTGGGCTGC TGTGGAGAGA TTCAACAATG AACAGAGACT ACGACTCCTT 
CAGTTTGTAA CAGGAACTTC CAGCATTCCC TATGAGGGCT TTGCCTCTCT GCGGGGCAGT 
AATGGTCCAC GCCGATTCTG TGTGGAGAAA TGGGGCAAAG TGACCTCCCT CCCACGGGCT 
CACACCTGCT TTAACCGACT GGATCTCCCT CCCTACCCCT CGTTCTCCAT GCTCTATGAG 
AAGATGGTCA CGGCTGTGGA GGAAACCAGC ACCTTTGGTC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2A ( XM_009304701.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302976.1 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48865
Clone ID Related Accession (Same CDS sequence)	XM_009304701.3 , XM_009304701.2 , XM_021478948.1
Accession Version	XM_009304701.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase HECW2-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304701.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCTC AGGGGCGGGA TCACCTGGCC ACACGGGAGT CTGCAGGCCG AAGACGCAGC 
CCGAATGTGC GGCACACGCT CAGCCCGGAA AACCTGCGAA GCCTGGCGGA GCGCGGTGGA 
GCTGAACAAG CGGGAGTAGG ACTGCTCAGA GCCAATAGCG ACACAGACCT GGTCAGTTCT 
CAAGGGCGAT CCTCACTCAC AGCCTCCACT CTAGAGTTTA CACTGTGCCG CGGACAGAAC 
CTCGTCATCT CATGGGACAT CAAGGAGGAG GTGGACGCCA CTGACTGGAT CGGCCTGTAC 
CACATAGATG AAACCTGTCC ATCAAATGTG TGGGACTGTA AGAACCGTGG GGTAAACGGC 
ACACAGTGTG GTCAGATCGT CTGGAGATTA GAGGTCGGGC CATACTTTCT TGAATCTGAG 
ACAAGGATCT GCTTTAAATA CTACCATGGA GTAAGTGGAG CATTGAGAGC CACGACTCCC 
TGCATCACAG TCAAGAACCC CAGAGCCTCG GTTGATGGCC AAGGAGAAGG ACAACAAGCT 
TCAGACAGTA GTCGAAAACT GGTTAGCTTT ACGCTATCAG ATATCCGAGC TGTTGGCCTG 
AAGAAGGGGA TGTTTTTTAA CCCAGACCCT TATCTGAAGA TGAGCATTCA TCCAGGCAAA 
AGAAGCTGCT TTCCGACCTT TTCCCATCAC GGCCAAGAGA GGCGATCCGG CATTACAACT 
AACACCACTA ATCCTGTTTG GCATGGAGAG AAACATACTT TTGTGGCTCT TATGACAGAT 
GTATTAGTGA TTGAGGTTAA AGACAAGTTT GCTAAAAGTC GACCCATTAT TAAACGCTTC 
TTGGGTCAGC TGAATATTCC TGTGCAGCTG CTGCTGGAGA GACATTCGAC AGGGAACCAG 
TCACTCAGCT TCTCCCTATG TCGTCGTTTG CCCACTGATC ATGTGAGTGG TCAAATGCAC 
TTTAAAGTGG ATGTCACGTC TATGGGCCCA GATGATGTGT CTCCCGAGAC AATTCTGGGG 
GCAGCAGCCC TCAATGGAGC TCCTGGAACC CCTTCAGATG ACGAAGAGCT GCCTCATCCT 
CTACCCATCT CATCAGCAGG CCCTTCCCCA ACAGCCTCCC TGGGGTCCAA TAGAAATGGG 
GAGGGGAGCA GCATTCCCTC CCCCGACACA GAAATCTATG GTGGCACCCT GGACGACGAG 
GCCCCTTCTT CTCCAGACCT GCTCCAGGGG TCTTTCAGCG AGCAGCTTGA TGCCATCGAG 
GCTCCTAAGG GCCCAGGTGA CAGACCTCTA GGAGCAGCTT CTCCAAAACT CCGCTCCAGC 
TTTCCCACAC ATACGCGACT CAGTGCCATG CTGCACATCG ACTCAGATGA AGATGAGGAC 
AGATCTGCTG CTACAGAGGC CACGTTGTCA ACATTTAACG GCATTCCAAG GTCTTATCAA 
ACACCCCCAA AAGCAGCAGC AAGAGGAGCT GTGTTTGATG GAGGCATAGA GGAACCTTCA 
GAAGAGGCTG GGCTGGAGTC TGAAGTAGAA ACTCTGAATG CAGGAGAAGA GGTGCCTCCT 
GAGCCAGAGG TCGTGCCCAG TTCAGTCGCT CCCGAGGTGG AAACCGCCAG TCTGATGGAG 
AGGCAGGAGG AACAGGGCCG GGAGGTGGAG GTAGAGGAGG AGGAGGAAGA GGGCCTGGCG 
CGCAGTGAAG AGTTGGCAAC CGAAGTTGAT ATCTCCTCAA TGGCATCTGA CTGCTGCCCG 
GGGCCTGCAT CAGCATCTCA GGAGGCGGAG CAGGGGGCAG AGGCAGCCAT TGACCCAGGG 
GGAGAAGATC TCTCAGATGA TCCACCTACC ACTTACGAGG TTGCAGAGAC TGCTGAAGAA 
ACACATCTAA CTAATGAGCC GGAGAGAGAG GCTCCAAAAC CAGAGCCCCC CACCAACGAG 
GAAGAGGAAT GTGAAGATGT GACTGAGGAT ACCATGTCCA GAAGATGGAG CCTGGAGGCC 
ACAGCAGGAG TGTCACAGGA GGATGAAGAT GAGTTGATAC CATCCGGAGA TGGGGAATCT 
GTGGACTCTG AGACTTTTGT TACGGAGACA GAGTCAGAAT TAGGCGTTCC TCAGGTAAAC 
GGAGATCAGC GTGTTAATTC TCTGCCATCT GTAAGACAAG ACATCCACCG ATACCAGCGT 
GTGGACGAGC CCTTACCTCC CAACTGGGAG GCTCGTATTG ACAGTCATGG TCGTATCTTC 
TTTGTGGATC ACGTGAATCG CACCACCACA TGGCAGAGAC CCACTGGCCC TCCGGCTCCA 
CAGGGTCTGA CCCGCTCCAG CTCCATTCTG CAGATGGAAC AACTCAACCG CAGGTATCAG 
AGTATTCGCA GGACAATGAC CAGTGAAAGG TCTGAGGAAC CCAGAGTTGA AGATCCTCCA 
GCTGATGAAA CAGACGTGCT GGCTCAGTCC ATCAGTGAAT ACCGGAGGGA TGGCATTATT 
GGTCAGTCCA GCTCACGCTC TCGTCTGGCT CTTCTGCTTC AGTCTCCTGG TGCAAAGTTC 
CTGTCCAGTC CAGACTTCTT CTCTGTTCTG CATTCTAATC CTAGTGCCTA TCGGATGTTC 
ACAAGTAACA CGTGTCTGAA GCACATGATC AGTAAAGTTC GACGGGATGC CCAACACTTT 
GAGCGGTACC AGCACAACCG AGACCTGGTC AACTTCCTCA ATCTGTTCTC CAACAAGCAG 
CTGGAGCTTC CCCGGGGCTG GGAGATGAAA CATGACCATA CTGGGAAGGC CTTCTTTGTA 
GACCACAACT GTCGTGCCAC AACATTTATT GACCCGCGGC TGCCCTTGCA GAACTCGCGG 
TCCAGCGGAT TGTTGGCTCA CAGACAGCAC CTGTCCCGCC AGCGCAGCCA CAGTGCAGGA 
GAGGTTGGAG ATGACTCTCG TTCCCCGAGT CCTCCAGTCC AGGGCCGGTC ATCTAGAAAC 
AGTCAGTATC AGGACCTGGT GCCTGTGGCC TATAATGATA AGATTGTTGC CTTTTTGCGA 
CAACCCAACA TTTTTGAGAT CCTGCAAGAG AGACAGCCAG AACTTGTGAG AAACCATTCC 
CTTAGGGAGA AGGTTCAGTT TATCCGCAAT GAGGGGGCAA CAGGGTTAAC TCGTCTGTCC 
AGTGATGCAG ACCTGGTTAT GTTGCTCAGC CTGTTTGAAG AGGAAGTGAT GTCGTATGTT 
CCGCCCAATG CCTTACTGCT CTCCAGCTAC TGTCATGCGT CCCCACAGAG CTCCCCTGGA 
ACCCCACGGG CTAATGCGCG AGCCCCTGCT CCCTACAAGC GGGATTTTGA GGCCAAATTA 
AGAAACTTCT ACCGCAAAAT GGAGACCAAG GGCTACGGAC AGGGACCAGG AAAAGTGAAG 
TTAATAATAA GAAGAGATCA CCTGCTTGAG GACGCTTTCA ATCAGATCAT GTGTTATTCC 
CGCAAAGACC TTCAAAGGAG CAGGCTTTAC GTCAGCTTTG TTGGAGAAGA AGGATTGGAC 
TATAGTGGTC CATCCAGGGA GTTCTTCTTC CTGGTTTCCC GTGAGCTTTT TAACCCATAC 
TATGGACTCT TTGAATACTC AGCTAATGAC ACTTACACTG TGCAGATCAG CCCTATGTCA 
GCCTTTGTGG ACAACCATCA TGAGTGGTTC CGGTTCAGTG GCCGCATTCT GGGTCTTGCT 
CTAATTCATC AGTACCTGCT GGATGCCTTC TTCACCCGAC CATTTTACAA AGGCCTTCTC 
CGCATTCCAT GTGATCTGAG TGATCTAGAG TTTCTGGATG AAGAGTTTCA TCAGAGTTTG 
CAGTGGATGA AGGATAATGA CATCGAGGAC ATGCTGGACC TCACCTTTAC AGTCAATGAG 
GAGGTGTTTG GACAGATAAC AGAACGTGAG CTAAAGCCGG GTGGATCTGG CATCGCTGTG 
TCAGACAAGA ACAAGAAAGA GTACATTGAG CGCATGGTGA AGTGGCGGAT TGAGAGAGGT 
GTGGCTCAGC AGACAGAGAG TCTTGTGCGA GGATTTTATG AGGTGGTGGA TGTGCGGCTC 
GTCTCAGTGT TTGATGCCCG AGAGCTGGAG CTGGTCATTG CTGGAACAGC AGAGATAGAC 
CTGTCCGACT GGAGAAACAA CACAGAATAC AGGGGAGGTT ATCATGACAA CCACATAGTG 
ATTCGATGGT TCTGGGCTGC TGTGGAGAGA TTCAACAATG AACAGAGACT ACGACTCCTT 
CAGTTTGTAA CAGGAACTTC CAGCATTCCC TATGAGGGCT TTGCCTCTCT GCGGGGCAGT 
AATGGTCCAC GCCGATTCTG TGTGGAGAAA TGGGGCAAAG TGACCTCCCT CCCACGGGCT 
CACACCTGCT TTAACCGACT GGATCTCCCT CCCTACCCCT CGTTCTCCAT GCTCTATGAG 
AAGATGGTCA CGGCTGTGGA GGAAACCAGC ACCTTTGGTC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302976.1
CDS	926..5353
Translation	MAPQGRDHLATRESAGRRRSPNVRHTLSPENLRSLAERGGAEQAGVGLLRANSDTDLVSSQGRSSLTASTLEFTLCRGQNLVISWDIKEEVDATDWIGLYHIDETCPSNVWDCKNRGVNGTQCGQIVWRLEVGPYFLESETRICFKYYHGVSGALRATTPCITVKNPRASVDGQGEGQQASDSSRKLVSFTLSDIRAVGLKKGMFFNPDPYLKMSIHPGKRSCFPTFSHHGQERRSGITTNTTNPVWHGEKHTFVALMTDVLVIEVKDKFAKSRPIIKRFLGQLNIPVQLLLERHSTGNQSLSFSLCRRLPTDHVSGQMHFKVDVTSMGPDDVSPETILGAAALNGAPGTPSDDEELPHPLPISSAGPSPTASLGSNRNGEGSSIPSPDTEIYGGTLDDEAPSSPDLLQGSFSEQLDAIEAPKGPGDRPLGAASPKLRSSFPTHTRLSAMLHIDSDEDEDRSAATEATLSTFNGIPRSYQTPPKAAARGAVFDGGIEEPSEEAGLESEVETLNAGEEVPPEPEVVPSSVAPEVETASLMERQEEQGREVEVEEEEEEGLARSEELATEVDISSMASDCCPGPASASQEAEQGAEAAIDPGGEDLSDDPPTTYEVAETAEETHLTNEPEREAPKPEPPTNEEEECEDVTEDTMSRRWSLEATAGVSQEDEDELIPSGDGESVDSETFVTETESELGVPQVNGDQRVNSLPSVRQDIHRYQRVDEPLPPNWEARIDSHGRIFFVDHVNRTTTWQRPTGPPAPQGLTRSSSILQMEQLNRRYQSIRRTMTSERSEEPRVEDPPADETDVLAQSISEYRRDGIIGQSSSRSRLALLLQSPGAKFLSSPDFFSVLHSNPSAYRMFTSNTCLKHMISKVRRDAQHFERYQHNRDLVNFLNLFSNKQLELPRGWEMKHDHTGKAFFVDHNCRATTFIDPRLPLQNSRSSGLLAHRQHLSRQRSHSAGEVGDDSRSPSPPVQGRSSRNSQYQDLVPVAYNDKIVAFLRQPNIFEILQERQPELVRNHSLREKVQFIRNEGATGLTRLSSDADLVMLLSLFEEEVMSYVPPNALLLSSYCHASPQSSPGTPRANARAPAPYKRDFEAKLRNFYRKMETKGYGQGPGKVKLIIRRDHLLEDAFNQIMCYSRKDLQRSRLYVSFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLRIPCDLSDLEFLDEEFHQSLQWMKDNDIEDMLDLTFTVNEEVFGQITERELKPGGSGIAVSDKNKKEYIERMVKWRIERGVAQQTESLVRGFYEVVDVRLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKVTSLPRAHTCFNRLDLPPYPSFSMLYEKMVTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009304701.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304701.2

ORF Insert Sequence:

ATGGCGCCTC AGGGGCGGGA TCACCTGGCC ACACGGGAGT CTGCAGGCCG AAGACGCAGC 
CCGAATGTGC GGCACACGCT CAGCCCGGAA AACCTGCGAA GCCTGGCGGA GCGCGGTGGA 
GCTGAACAAG CGGGAGTAGG ACTGCTCAGA GCCAATAGCG ACACAGACCT GGTCAGTTCT 
CAAGGGCGAT CCTCACTCAC AGCCTCCACT CTAGAGTTTA CACTGTGCCG CGGACAGAAC 
CTCGTCATCT CATGGGACAT CAAGGAGGAG GTGGACGCCA CTGACTGGAT CGGCCTGTAC 
CACATAGATG AAACCTGTCC ATCAAATGTG TGGGACTGTA AGAACCGTGG GGTAAACGGC 
ACACAGTGTG GTCAGATCGT CTGGAGATTA GAGGTCGGGC CATACTTTCT TGAATCTGAG 
ACAAGGATCT GCTTTAAATA CTACCATGGA GTAAGTGGAG CATTGAGAGC CACGACTCCC 
TGCATCACAG TCAAGAACCC CAGAGCCTCG GTTGATGGCC AAGGAGAAGG ACAACAAGCT 
TCAGACAGTA GTCGAAAACT GGTTAGCTTT ACGCTATCAG ATATCCGAGC TGTTGGCCTG 
AAGAAGGGGA TGTTTTTTAA CCCAGACCCT TATCTGAAGA TGAGCATTCA TCCAGGCAAA 
AGAAGCTGCT TTCCGACCTT TTCCCATCAC GGCCAAGAGA GGCGATCCGG CATTACAACT 
AACACCACTA ATCCTGTTTG GCATGGAGAG AAACATACTT TTGTGGCTCT TATGACAGAT 
GTATTAGTGA TTGAGGTTAA AGACAAGTTT GCTAAAAGTC GACCCATTAT TAAACGCTTC 
TTGGGTCAGC TGAATATTCC TGTGCAGCTG CTGCTGGAGA GACATTCGAC AGGGAACCAG 
TCACTCAGCT TCTCCCTATG TCGTCGTTTG CCCACTGATC ATGTGAGTGG TCAAATGCAC 
TTTAAAGTGG ATGTCACGTC TATGGGCCCA GATGATGTGT CTCCCGAGAC AATTCTGGGG 
GCAGCAGCCC TCAATGGAGC TCCTGGAACC CCTTCAGATG ACGAAGAGCT GCCTCATCCT 
CTACCCATCT CATCAGCAGG CCCTTCCCCA ACAGCCTCCC TGGGGTCCAA TAGAAATGGG 
GAGGGGAGCA GCATTCCCTC CCCCGACACA GAAATCTATG GTGGCACCCT GGACGACGAG 
GCCCCTTCTT CTCCAGACCT GCTCCAGGGG TCTTTCAGCG AGCAGCTTGA TGCCATCGAG 
GCTCCTAAGG GCCCAGGTGA CAGACCTCTA GGAGCAGCTT CTCCAAAACT CCGCTCCAGC 
TTTCCCACAC ATACGCGACT CAGTGCCATG CTGCACATCG ACTCAGATGA AGATGAGGAC 
AGATCTGCTG CTACAGAGGC CACGTTGTCA ACATTTAACG GCATTCCAAG GTCTTATCAA 
ACACCCCCAA AAGCAGCAGC AAGAGGAGCT GTGTTTGATG GAGGCATAGA GGAACCTTCA 
GAAGAGGCTG GGCTGGAGTC TGAAGTAGAA ACTCTGAATG CAGGAGAAGA GGTGCCTCCT 
GAGCCAGAGG TCGTGCCCAG TTCAGTCGCT CCCGAGGTGG AAACCGCCAG TCTGATGGAG 
AGGCAGGAGG AACAGGGCCG GGAGGTGGAG GTAGAGGAGG AGGAGGAAGA GGGCCTGGCG 
CGCAGTGAAG AGTTGGCAAC CGAAGTTGAT ATCTCCTCAA TGGCATCTGA CTGCTGCCCG 
GGGCCTGCAT CAGCATCTCA GGAGGCGGAG CAGGGGGCAG AGGCAGCCAT TGACCCAGGG 
GGAGAAGATC TCTCAGATGA TCCACCTACC ACTTACGAGG TTGCAGAGAC TGCTGAAGAA 
ACACATCTAA CTAATGAGCC GGAGAGAGAG GCTCCAAAAC CAGAGCCCCC CACCAACGAG 
GAAGAGGAAT GTGAAGATGT GACTGAGGAT ACCATGTCCA GAAGATGGAG CCTGGAGGCC 
ACAGCAGGAG TGTCACAGGA GGATGAAGAT GAGTTGATAC CATCCGGAGA TGGGGAATCT 
GTGGACTCTG AGACTTTTGT TACGGAGACA GAGTCAGAAT TAGGCGTTCC TCAGGTAAAC 
GGAGATCAGC GTGTTAATTC TCTGCCATCT GTAAGACAAG ACATCCACCG ATACCAGCGT 
GTGGACGAGC CCTTACCTCC CAACTGGGAG GCTCGTATTG ACAGTCATGG TCGTATCTTC 
TTTGTGGATC ACGTGAATCG CACCACCACA TGGCAGAGAC CCACTGGCCC TCCGGCTCCA 
CAGGGTCTGA CCCGCTCCAG CTCCATTCTG CAGATGGAAC AACTCAACCG CAGGTATCAG 
AGTATTCGCA GGACAATGAC CAGTGAAAGG TCTGAGGAAC CCAGAGTTGA AGATCCTCCA 
GCTGATGAAA CAGACGTGCT GGCTCAGTCC ATCAGTGAAT ACCGGAGGGA TGGCATTATT 
GGTCAGTCCA GCTCACGCTC TCGTCTGGCT CTTCTGCTTC AGTCTCCTGG TGCAAAGTTC 
CTGTCCAGTC CAGACTTCTT CTCTGTTCTG CATTCTAATC CTAGTGCCTA TCGGATGTTC 
ACAAGTAACA CGTGTCTGAA GCACATGATC AGTAAAGTTC GACGGGATGC CCAACACTTT 
GAGCGGTACC AGCACAACCG AGACCTGGTC AACTTCCTCA ATCTGTTCTC CAACAAGCAG 
CTGGAGCTTC CCCGGGGCTG GGAGATGAAA CATGACCATA CTGGGAAGGC CTTCTTTGTA 
GACCACAACT GTCGTGCCAC AACATTTATT GACCCGCGGC TGCCCTTGCA GAACTCGCGG 
TCCAGCGGAT TGTTGGCTCA CAGACAGCAC CTGTCCCGCC AGCGCAGCCA CAGTGCAGGA 
GAGGTTGGAG ATGACTCTCG TTCCCCGAGT CCTCCAGTCC AGGGCCGGTC ATCTAGAAAC 
AGTCAGTATC AGGACCTGGT GCCTGTGGCC TATAATGATA AGATTGTTGC CTTTTTGCGA 
CAACCCAACA TTTTTGAGAT CCTGCAAGAG AGACAGCCAG AACTTGTGAG AAACCATTCC 
CTTAGGGAGA AGGTTCAGTT TATCCGCAAT GAGGGGGCAA CAGGGTTAAC TCGTCTGTCC 
AGTGATGCAG ACCTGGTTAT GTTGCTCAGC CTGTTTGAAG AGGAAGTGAT GTCGTATGTT 
CCGCCCAATG CCTTACTGCT CTCCAGCTAC TGTCATGCGT CCCCACAGAG CTCCCCTGGA 
ACCCCACGGG CTAATGCGCG AGCCCCTGCT CCCTACAAGC GGGATTTTGA GGCCAAATTA 
AGAAACTTCT ACCGCAAAAT GGAGACCAAG GGCTACGGAC AGGGACCAGG AAAAGTGAAG 
TTAATAATAA GAAGAGATCA CCTGCTTGAG GACGCTTTCA ATCAGATCAT GTGTTATTCC 
CGCAAAGACC TTCAAAGGAG CAGGCTTTAC GTCAGCTTTG TTGGAGAAGA AGGATTGGAC 
TATAGTGGTC CATCCAGGGA GTTCTTCTTC CTGGTTTCCC GTGAGCTTTT TAACCCATAC 
TATGGACTCT TTGAATACTC AGCTAATGAC ACTTACACTG TGCAGATCAG CCCTATGTCA 
GCCTTTGTGG ACAACCATCA TGAGTGGTTC CGGTTCAGTG GCCGCATTCT GGGTCTTGCT 
CTAATTCATC AGTACCTGCT GGATGCCTTC TTCACCCGAC CATTTTACAA AGGCCTTCTC 
CGCATTCCAT GTGATCTGAG TGATCTAGAG TTTCTGGATG AAGAGTTTCA TCAGAGTTTG 
CAGTGGATGA AGGATAATGA CATCGAGGAC ATGCTGGACC TCACCTTTAC AGTCAATGAG 
GAGGTGTTTG GACAGATAAC AGAACGTGAG CTAAAGCCGG GTGGATCTGG CATCGCTGTG 
TCAGACAAGA ACAAGAAAGA GTACATTGAG CGCATGGTGA AGTGGCGGAT TGAGAGAGGT 
GTGGCTCAGC AGACAGAGAG TCTTGTGCGA GGATTTTATG AGGTGGTGGA TGTGCGGCTC 
GTCTCAGTGT TTGATGCCCG AGAGCTGGAG CTGGTCATTG CTGGAACAGC AGAGATAGAC 
CTGTCCGACT GGAGAAACAA CACAGAATAC AGGGGAGGTT ATCATGACAA CCACATAGTG 
ATTCGATGGT TCTGGGCTGC TGTGGAGAGA TTCAACAATG AACAGAGACT ACGACTCCTT 
CAGTTTGTAA CAGGAACTTC CAGCATTCCC TATGAGGGCT TTGCCTCTCT GCGGGGCAGT 
AATGGTCCAC GCCGATTCTG TGTGGAGAAA TGGGGCAAAG TGACCTCCCT CCCACGGGCT 
CACACCTGCT TTAACCGACT GGATCTCCCT CCCTACCCCT CGTTCTCCAT GCTCTATGAG 
AAGATGGTCA CGGCTGTGGA GGAAACCAGC ACCTTTGGTC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2A ( XM_021478948.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021334623.1 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X1, mRNA.

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CloneID	ODa48865
Clone ID Related Accession (Same CDS sequence)	XM_009304701.3 , XM_009304701.2 , XM_021478948.1
Accession Version	XM_021478948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase HECW2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCTC AGGGGCGGGA TCACCTGGCC ACACGGGAGT CTGCAGGCCG AAGACGCAGC 
CCGAATGTGC GGCACACGCT CAGCCCGGAA AACCTGCGAA GCCTGGCGGA GCGCGGTGGA 
GCTGAACAAG CGGGAGTAGG ACTGCTCAGA GCCAATAGCG ACACAGACCT GGTCAGTTCT 
CAAGGGCGAT CCTCACTCAC AGCCTCCACT CTAGAGTTTA CACTGTGCCG CGGACAGAAC 
CTCGTCATCT CATGGGACAT CAAGGAGGAG GTGGACGCCA CTGACTGGAT CGGCCTGTAC 
CACATAGATG AAACCTGTCC ATCAAATGTG TGGGACTGTA AGAACCGTGG GGTAAACGGC 
ACACAGTGTG GTCAGATCGT CTGGAGATTA GAGGTCGGGC CATACTTTCT TGAATCTGAG 
ACAAGGATCT GCTTTAAATA CTACCATGGA GTAAGTGGAG CATTGAGAGC CACGACTCCC 
TGCATCACAG TCAAGAACCC CAGAGCCTCG GTTGATGGCC AAGGAGAAGG ACAACAAGCT 
TCAGACAGTA GTCGAAAACT GGTTAGCTTT ACGCTATCAG ATATCCGAGC TGTTGGCCTG 
AAGAAGGGGA TGTTTTTTAA CCCAGACCCT TATCTGAAGA TGAGCATTCA TCCAGGCAAA 
AGAAGCTGCT TTCCGACCTT TTCCCATCAC GGCCAAGAGA GGCGATCCGG CATTACAACT 
AACACCACTA ATCCTGTTTG GCATGGAGAG AAACATACTT TTGTGGCTCT TATGACAGAT 
GTATTAGTGA TTGAGGTTAA AGACAAGTTT GCTAAAAGTC GACCCATTAT TAAACGCTTC 
TTGGGTCAGC TGAATATTCC TGTGCAGCTG CTGCTGGAGA GACATTCGAC AGGGAACCAG 
TCACTCAGCT TCTCCCTATG TCGTCGTTTG CCCACTGATC ATGTGAGTGG TCAAATGCAC 
TTTAAAGTGG ATGTCACGTC TATGGGCCCA GATGATGTGT CTCCCGAGAC AATTCTGGGG 
GCAGCAGCCC TCAATGGAGC TCCTGGAACC CCTTCAGATG ACGAAGAGCT GCCTCATCCT 
CTACCCATCT CATCAGCAGG CCCTTCCCCA ACAGCCTCCC TGGGGTCCAA TAGAAATGGG 
GAGGGGAGCA GCATTCCCTC CCCCGACACA GAAATCTATG GTGGCACCCT GGACGACGAG 
GCCCCTTCTT CTCCAGACCT GCTCCAGGGG TCTTTCAGCG AGCAGCTTGA TGCCATCGAG 
GCTCCTAAGG GCCCAGGTGA CAGACCTCTA GGAGCAGCTT CTCCAAAACT CCGCTCCAGC 
TTTCCCACAC ATACGCGACT CAGTGCCATG CTGCACATCG ACTCAGATGA AGATGAGGAC 
AGATCTGCTG CTACAGAGGC CACGTTGTCA ACATTTAACG GCATTCCAAG GTCTTATCAA 
ACACCCCCAA AAGCAGCAGC AAGAGGAGCT GTGTTTGATG GAGGCATAGA GGAACCTTCA 
GAAGAGGCTG GGCTGGAGTC TGAAGTAGAA ACTCTGAATG CAGGAGAAGA GGTGCCTCCT 
GAGCCAGAGG TCGTGCCCAG TTCAGTCGCT CCCGAGGTGG AAACCGCCAG TCTGATGGAG 
AGGCAGGAGG AACAGGGCCG GGAGGTGGAG GTAGAGGAGG AGGAGGAAGA GGGCCTGGCG 
CGCAGTGAAG AGTTGGCAAC CGAAGTTGAT ATCTCCTCAA TGGCATCTGA CTGCTGCCCG 
GGGCCTGCAT CAGCATCTCA GGAGGCGGAG CAGGGGGCAG AGGCAGCCAT TGACCCAGGG 
GGAGAAGATC TCTCAGATGA TCCACCTACC ACTTACGAGG TTGCAGAGAC TGCTGAAGAA 
ACACATCTAA CTAATGAGCC GGAGAGAGAG GCTCCAAAAC CAGAGCCCCC CACCAACGAG 
GAAGAGGAAT GTGAAGATGT GACTGAGGAT ACCATGTCCA GAAGATGGAG CCTGGAGGCC 
ACAGCAGGAG TGTCACAGGA GGATGAAGAT GAGTTGATAC CATCCGGAGA TGGGGAATCT 
GTGGACTCTG AGACTTTTGT TACGGAGACA GAGTCAGAAT TAGGCGTTCC TCAGGTAAAC 
GGAGATCAGC GTGTTAATTC TCTGCCATCT GTAAGACAAG ACATCCACCG ATACCAGCGT 
GTGGACGAGC CCTTACCTCC CAACTGGGAG GCTCGTATTG ACAGTCATGG TCGTATCTTC 
TTTGTGGATC ACGTGAATCG CACCACCACA TGGCAGAGAC CCACTGGCCC TCCGGCTCCA 
CAGGGTCTGA CCCGCTCCAG CTCCATTCTG CAGATGGAAC AACTCAACCG CAGGTATCAG 
AGTATTCGCA GGACAATGAC CAGTGAAAGG TCTGAGGAAC CCAGAGTTGA AGATCCTCCA 
GCTGATGAAA CAGACGTGCT GGCTCAGTCC ATCAGTGAAT ACCGGAGGGA TGGCATTATT 
GGTCAGTCCA GCTCACGCTC TCGTCTGGCT CTTCTGCTTC AGTCTCCTGG TGCAAAGTTC 
CTGTCCAGTC CAGACTTCTT CTCTGTTCTG CATTCTAATC CTAGTGCCTA TCGGATGTTC 
ACAAGTAACA CGTGTCTGAA GCACATGATC AGTAAAGTTC GACGGGATGC CCAACACTTT 
GAGCGGTACC AGCACAACCG AGACCTGGTC AACTTCCTCA ATCTGTTCTC CAACAAGCAG 
CTGGAGCTTC CCCGGGGCTG GGAGATGAAA CATGACCATA CTGGGAAGGC CTTCTTTGTA 
GACCACAACT GTCGTGCCAC AACATTTATT GACCCGCGGC TGCCCTTGCA GAACTCGCGG 
TCCAGCGGAT TGTTGGCTCA CAGACAGCAC CTGTCCCGCC AGCGCAGCCA CAGTGCAGGA 
GAGGTTGGAG ATGACTCTCG TTCCCCGAGT CCTCCAGTCC AGGGCCGGTC ATCTAGAAAC 
AGTCAGTATC AGGACCTGGT GCCTGTGGCC TATAATGATA AGATTGTTGC CTTTTTGCGA 
CAACCCAACA TTTTTGAGAT CCTGCAAGAG AGACAGCCAG AACTTGTGAG AAACCATTCC 
CTTAGGGAGA AGGTTCAGTT TATCCGCAAT GAGGGGGCAA CAGGGTTAAC TCGTCTGTCC 
AGTGATGCAG ACCTGGTTAT GTTGCTCAGC CTGTTTGAAG AGGAAGTGAT GTCGTATGTT 
CCGCCCAATG CCTTACTGCT CTCCAGCTAC TGTCATGCGT CCCCACAGAG CTCCCCTGGA 
ACCCCACGGG CTAATGCGCG AGCCCCTGCT CCCTACAAGC GGGATTTTGA GGCCAAATTA 
AGAAACTTCT ACCGCAAAAT GGAGACCAAG GGCTACGGAC AGGGACCAGG AAAAGTGAAG 
TTAATAATAA GAAGAGATCA CCTGCTTGAG GACGCTTTCA ATCAGATCAT GTGTTATTCC 
CGCAAAGACC TTCAAAGGAG CAGGCTTTAC GTCAGCTTTG TTGGAGAAGA AGGATTGGAC 
TATAGTGGTC CATCCAGGGA GTTCTTCTTC CTGGTTTCCC GTGAGCTTTT TAACCCATAC 
TATGGACTCT TTGAATACTC AGCTAATGAC ACTTACACTG TGCAGATCAG CCCTATGTCA 
GCCTTTGTGG ACAACCATCA TGAGTGGTTC CGGTTCAGTG GCCGCATTCT GGGTCTTGCT 
CTAATTCATC AGTACCTGCT GGATGCCTTC TTCACCCGAC CATTTTACAA AGGCCTTCTC 
CGCATTCCAT GTGATCTGAG TGATCTAGAG TTTCTGGATG AAGAGTTTCA TCAGAGTTTG 
CAGTGGATGA AGGATAATGA CATCGAGGAC ATGCTGGACC TCACCTTTAC AGTCAATGAG 
GAGGTGTTTG GACAGATAAC AGAACGTGAG CTAAAGCCGG GTGGATCTGG CATCGCTGTG 
TCAGACAAGA ACAAGAAAGA GTACATTGAG CGCATGGTGA AGTGGCGGAT TGAGAGAGGT 
GTGGCTCAGC AGACAGAGAG TCTTGTGCGA GGATTTTATG AGGTGGTGGA TGTGCGGCTC 
GTCTCAGTGT TTGATGCCCG AGAGCTGGAG CTGGTCATTG CTGGAACAGC AGAGATAGAC 
CTGTCCGACT GGAGAAACAA CACAGAATAC AGGGGAGGTT ATCATGACAA CCACATAGTG 
ATTCGATGGT TCTGGGCTGC TGTGGAGAGA TTCAACAATG AACAGAGACT ACGACTCCTT 
CAGTTTGTAA CAGGAACTTC CAGCATTCCC TATGAGGGCT TTGCCTCTCT GCGGGGCAGT 
AATGGTCCAC GCCGATTCTG TGTGGAGAAA TGGGGCAAAG TGACCTCCCT CCCACGGGCT 
CACACCTGCT TTAACCGACT GGATCTCCCT CCCTACCCCT CGTTCTCCAT GCTCTATGAG 
AAGATGGTCA CGGCTGTGGA GGAAACCAGC ACCTTTGGTC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334623.1
CDS	1071..5498
Translation	MAPQGRDHLATRESAGRRRSPNVRHTLSPENLRSLAERGGAEQAGVGLLRANSDTDLVSSQGRSSLTASTLEFTLCRGQNLVISWDIKEEVDATDWIGLYHIDETCPSNVWDCKNRGVNGTQCGQIVWRLEVGPYFLESETRICFKYYHGVSGALRATTPCITVKNPRASVDGQGEGQQASDSSRKLVSFTLSDIRAVGLKKGMFFNPDPYLKMSIHPGKRSCFPTFSHHGQERRSGITTNTTNPVWHGEKHTFVALMTDVLVIEVKDKFAKSRPIIKRFLGQLNIPVQLLLERHSTGNQSLSFSLCRRLPTDHVSGQMHFKVDVTSMGPDDVSPETILGAAALNGAPGTPSDDEELPHPLPISSAGPSPTASLGSNRNGEGSSIPSPDTEIYGGTLDDEAPSSPDLLQGSFSEQLDAIEAPKGPGDRPLGAASPKLRSSFPTHTRLSAMLHIDSDEDEDRSAATEATLSTFNGIPRSYQTPPKAAARGAVFDGGIEEPSEEAGLESEVETLNAGEEVPPEPEVVPSSVAPEVETASLMERQEEQGREVEVEEEEEEGLARSEELATEVDISSMASDCCPGPASASQEAEQGAEAAIDPGGEDLSDDPPTTYEVAETAEETHLTNEPEREAPKPEPPTNEEEECEDVTEDTMSRRWSLEATAGVSQEDEDELIPSGDGESVDSETFVTETESELGVPQVNGDQRVNSLPSVRQDIHRYQRVDEPLPPNWEARIDSHGRIFFVDHVNRTTTWQRPTGPPAPQGLTRSSSILQMEQLNRRYQSIRRTMTSERSEEPRVEDPPADETDVLAQSISEYRRDGIIGQSSSRSRLALLLQSPGAKFLSSPDFFSVLHSNPSAYRMFTSNTCLKHMISKVRRDAQHFERYQHNRDLVNFLNLFSNKQLELPRGWEMKHDHTGKAFFVDHNCRATTFIDPRLPLQNSRSSGLLAHRQHLSRQRSHSAGEVGDDSRSPSPPVQGRSSRNSQYQDLVPVAYNDKIVAFLRQPNIFEILQERQPELVRNHSLREKVQFIRNEGATGLTRLSSDADLVMLLSLFEEEVMSYVPPNALLLSSYCHASPQSSPGTPRANARAPAPYKRDFEAKLRNFYRKMETKGYGQGPGKVKLIIRRDHLLEDAFNQIMCYSRKDLQRSRLYVSFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLRIPCDLSDLEFLDEEFHQSLQWMKDNDIEDMLDLTFTVNEEVFGQITERELKPGGSGIAVSDKNKKEYIERMVKWRIERGVAQQTESLVRGFYEVVDVRLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKVTSLPRAHTCFNRLDLPPYPSFSMLYEKMVTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_021478948.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478948.1

ORF Insert Sequence:

ATGGCGCCTC AGGGGCGGGA TCACCTGGCC ACACGGGAGT CTGCAGGCCG AAGACGCAGC 
CCGAATGTGC GGCACACGCT CAGCCCGGAA AACCTGCGAA GCCTGGCGGA GCGCGGTGGA 
GCTGAACAAG CGGGAGTAGG ACTGCTCAGA GCCAATAGCG ACACAGACCT GGTCAGTTCT 
CAAGGGCGAT CCTCACTCAC AGCCTCCACT CTAGAGTTTA CACTGTGCCG CGGACAGAAC 
CTCGTCATCT CATGGGACAT CAAGGAGGAG GTGGACGCCA CTGACTGGAT CGGCCTGTAC 
CACATAGATG AAACCTGTCC ATCAAATGTG TGGGACTGTA AGAACCGTGG GGTAAACGGC 
ACACAGTGTG GTCAGATCGT CTGGAGATTA GAGGTCGGGC CATACTTTCT TGAATCTGAG 
ACAAGGATCT GCTTTAAATA CTACCATGGA GTAAGTGGAG CATTGAGAGC CACGACTCCC 
TGCATCACAG TCAAGAACCC CAGAGCCTCG GTTGATGGCC AAGGAGAAGG ACAACAAGCT 
TCAGACAGTA GTCGAAAACT GGTTAGCTTT ACGCTATCAG ATATCCGAGC TGTTGGCCTG 
AAGAAGGGGA TGTTTTTTAA CCCAGACCCT TATCTGAAGA TGAGCATTCA TCCAGGCAAA 
AGAAGCTGCT TTCCGACCTT TTCCCATCAC GGCCAAGAGA GGCGATCCGG CATTACAACT 
AACACCACTA ATCCTGTTTG GCATGGAGAG AAACATACTT TTGTGGCTCT TATGACAGAT 
GTATTAGTGA TTGAGGTTAA AGACAAGTTT GCTAAAAGTC GACCCATTAT TAAACGCTTC 
TTGGGTCAGC TGAATATTCC TGTGCAGCTG CTGCTGGAGA GACATTCGAC AGGGAACCAG 
TCACTCAGCT TCTCCCTATG TCGTCGTTTG CCCACTGATC ATGTGAGTGG TCAAATGCAC 
TTTAAAGTGG ATGTCACGTC TATGGGCCCA GATGATGTGT CTCCCGAGAC AATTCTGGGG 
GCAGCAGCCC TCAATGGAGC TCCTGGAACC CCTTCAGATG ACGAAGAGCT GCCTCATCCT 
CTACCCATCT CATCAGCAGG CCCTTCCCCA ACAGCCTCCC TGGGGTCCAA TAGAAATGGG 
GAGGGGAGCA GCATTCCCTC CCCCGACACA GAAATCTATG GTGGCACCCT GGACGACGAG 
GCCCCTTCTT CTCCAGACCT GCTCCAGGGG TCTTTCAGCG AGCAGCTTGA TGCCATCGAG 
GCTCCTAAGG GCCCAGGTGA CAGACCTCTA GGAGCAGCTT CTCCAAAACT CCGCTCCAGC 
TTTCCCACAC ATACGCGACT CAGTGCCATG CTGCACATCG ACTCAGATGA AGATGAGGAC 
AGATCTGCTG CTACAGAGGC CACGTTGTCA ACATTTAACG GCATTCCAAG GTCTTATCAA 
ACACCCCCAA AAGCAGCAGC AAGAGGAGCT GTGTTTGATG GAGGCATAGA GGAACCTTCA 
GAAGAGGCTG GGCTGGAGTC TGAAGTAGAA ACTCTGAATG CAGGAGAAGA GGTGCCTCCT 
GAGCCAGAGG TCGTGCCCAG TTCAGTCGCT CCCGAGGTGG AAACCGCCAG TCTGATGGAG 
AGGCAGGAGG AACAGGGCCG GGAGGTGGAG GTAGAGGAGG AGGAGGAAGA GGGCCTGGCG 
CGCAGTGAAG AGTTGGCAAC CGAAGTTGAT ATCTCCTCAA TGGCATCTGA CTGCTGCCCG 
GGGCCTGCAT CAGCATCTCA GGAGGCGGAG CAGGGGGCAG AGGCAGCCAT TGACCCAGGG 
GGAGAAGATC TCTCAGATGA TCCACCTACC ACTTACGAGG TTGCAGAGAC TGCTGAAGAA 
ACACATCTAA CTAATGAGCC GGAGAGAGAG GCTCCAAAAC CAGAGCCCCC CACCAACGAG 
GAAGAGGAAT GTGAAGATGT GACTGAGGAT ACCATGTCCA GAAGATGGAG CCTGGAGGCC 
ACAGCAGGAG TGTCACAGGA GGATGAAGAT GAGTTGATAC CATCCGGAGA TGGGGAATCT 
GTGGACTCTG AGACTTTTGT TACGGAGACA GAGTCAGAAT TAGGCGTTCC TCAGGTAAAC 
GGAGATCAGC GTGTTAATTC TCTGCCATCT GTAAGACAAG ACATCCACCG ATACCAGCGT 
GTGGACGAGC CCTTACCTCC CAACTGGGAG GCTCGTATTG ACAGTCATGG TCGTATCTTC 
TTTGTGGATC ACGTGAATCG CACCACCACA TGGCAGAGAC CCACTGGCCC TCCGGCTCCA 
CAGGGTCTGA CCCGCTCCAG CTCCATTCTG CAGATGGAAC AACTCAACCG CAGGTATCAG 
AGTATTCGCA GGACAATGAC CAGTGAAAGG TCTGAGGAAC CCAGAGTTGA AGATCCTCCA 
GCTGATGAAA CAGACGTGCT GGCTCAGTCC ATCAGTGAAT ACCGGAGGGA TGGCATTATT 
GGTCAGTCCA GCTCACGCTC TCGTCTGGCT CTTCTGCTTC AGTCTCCTGG TGCAAAGTTC 
CTGTCCAGTC CAGACTTCTT CTCTGTTCTG CATTCTAATC CTAGTGCCTA TCGGATGTTC 
ACAAGTAACA CGTGTCTGAA GCACATGATC AGTAAAGTTC GACGGGATGC CCAACACTTT 
GAGCGGTACC AGCACAACCG AGACCTGGTC AACTTCCTCA ATCTGTTCTC CAACAAGCAG 
CTGGAGCTTC CCCGGGGCTG GGAGATGAAA CATGACCATA CTGGGAAGGC CTTCTTTGTA 
GACCACAACT GTCGTGCCAC AACATTTATT GACCCGCGGC TGCCCTTGCA GAACTCGCGG 
TCCAGCGGAT TGTTGGCTCA CAGACAGCAC CTGTCCCGCC AGCGCAGCCA CAGTGCAGGA 
GAGGTTGGAG ATGACTCTCG TTCCCCGAGT CCTCCAGTCC AGGGCCGGTC ATCTAGAAAC 
AGTCAGTATC AGGACCTGGT GCCTGTGGCC TATAATGATA AGATTGTTGC CTTTTTGCGA 
CAACCCAACA TTTTTGAGAT CCTGCAAGAG AGACAGCCAG AACTTGTGAG AAACCATTCC 
CTTAGGGAGA AGGTTCAGTT TATCCGCAAT GAGGGGGCAA CAGGGTTAAC TCGTCTGTCC 
AGTGATGCAG ACCTGGTTAT GTTGCTCAGC CTGTTTGAAG AGGAAGTGAT GTCGTATGTT 
CCGCCCAATG CCTTACTGCT CTCCAGCTAC TGTCATGCGT CCCCACAGAG CTCCCCTGGA 
ACCCCACGGG CTAATGCGCG AGCCCCTGCT CCCTACAAGC GGGATTTTGA GGCCAAATTA 
AGAAACTTCT ACCGCAAAAT GGAGACCAAG GGCTACGGAC AGGGACCAGG AAAAGTGAAG 
TTAATAATAA GAAGAGATCA CCTGCTTGAG GACGCTTTCA ATCAGATCAT GTGTTATTCC 
CGCAAAGACC TTCAAAGGAG CAGGCTTTAC GTCAGCTTTG TTGGAGAAGA AGGATTGGAC 
TATAGTGGTC CATCCAGGGA GTTCTTCTTC CTGGTTTCCC GTGAGCTTTT TAACCCATAC 
TATGGACTCT TTGAATACTC AGCTAATGAC ACTTACACTG TGCAGATCAG CCCTATGTCA 
GCCTTTGTGG ACAACCATCA TGAGTGGTTC CGGTTCAGTG GCCGCATTCT GGGTCTTGCT 
CTAATTCATC AGTACCTGCT GGATGCCTTC TTCACCCGAC CATTTTACAA AGGCCTTCTC 
CGCATTCCAT GTGATCTGAG TGATCTAGAG TTTCTGGATG AAGAGTTTCA TCAGAGTTTG 
CAGTGGATGA AGGATAATGA CATCGAGGAC ATGCTGGACC TCACCTTTAC AGTCAATGAG 
GAGGTGTTTG GACAGATAAC AGAACGTGAG CTAAAGCCGG GTGGATCTGG CATCGCTGTG 
TCAGACAAGA ACAAGAAAGA GTACATTGAG CGCATGGTGA AGTGGCGGAT TGAGAGAGGT 
GTGGCTCAGC AGACAGAGAG TCTTGTGCGA GGATTTTATG AGGTGGTGGA TGTGCGGCTC 
GTCTCAGTGT TTGATGCCCG AGAGCTGGAG CTGGTCATTG CTGGAACAGC AGAGATAGAC 
CTGTCCGACT GGAGAAACAA CACAGAATAC AGGGGAGGTT ATCATGACAA CCACATAGTG 
ATTCGATGGT TCTGGGCTGC TGTGGAGAGA TTCAACAATG AACAGAGACT ACGACTCCTT 
CAGTTTGTAA CAGGAACTTC CAGCATTCCC TATGAGGGCT TTGCCTCTCT GCGGGGCAGT 
AATGGTCCAC GCCGATTCTG TGTGGAGAAA TGGGGCAAAG TGACCTCCCT CCCACGGGCT 
CACACCTGCT TTAACCGACT GGATCTCCCT CCCTACCCCT CGTTCTCCAT GCTCTATGAG 
AAGATGGTCA CGGCTGTGGA GGAAACCAGC ACCTTTGGTC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2A ( XM_021478949.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021334624.1 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X2, mRNA.

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CloneID	ODa48866
Clone ID Related Accession (Same CDS sequence)	XM_021478949.1 , XM_009304702.3 , XM_009304702.2
Accession Version	XM_021478949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase HECW2-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCTC AGGGGCGGGA TCACCTGGCC ACACGGGAGT CTGCAGGCCG AAGACGCAGC 
CCGAATGTGC GGCACACGCT CAGCCCGGAA AACCTGCGAA GCCTGGCGGA GCGCGGTGGA 
GCTGAACAAG CGGGAGTAGG ACTGCTCAGA GCCAATAGCG ACACAGACCT GGTCAGTTCT 
CAAGGGCGAT CCTCACTCAC AGCCTCCACT CTAGAGTTTA CACTGTGCCG CGGACAGAAC 
CTCGTCATCT CATGGGACAT CAAGGAGGAG GTGGACGCCA CTGACTGGAT CGGCCTGTAC 
CACATAGATG AAACCTGTCC ATCAAATGTG TGGGACTGTA AGAACCGTGG GGTAAACGGC 
ACACAGTGTG GTCAGATCGT CTGGAGATTA GAGGTCGGGC CATACTTTCT TGAATCTGAG 
ACAAGGATCT GCTTTAAATA CTACCATGGA GTAAGTGGAG CATTGAGAGC CACGACTCCC 
TGCATCACAG TCAAGAACCC CAGAGCCTCG GTTGATGGCC AAGGAGAAGG ACAACAAGCT 
TCAGACAGTA GTCGAAAACT GGTTAGCTTT ACGCTATCAG ATATCCGAGC TGTTGGCCTG 
AAGAAGGGGA TGTTTTTTAA CCCAGACCCT TATCTGAAGA TGAGCATTCA TCCAGGCAAA 
AGAAGCTGCT TTCCGACCTT TTCCCATCAC GGCCAAGAGA GGCGATCCGG CATTACAACT 
AACACCACTA ATCCTGTTTG GCATGGAGAG AAACATACTT TTGTGGCTCT TATGACAGAT 
GTATTAGTGA TTGAGGTTAA AGACAAGTTT GCTAAAAGTC GACCCATTAT TAAACGCTTC 
TTGGGTCAGC TGAATATTCC TGTGCAGCTG CTGCTGGAGA GACATTCGAC AGGGAACCAG 
TCACTCAGCT TCTCCCTATG TCGTCGTTTG CCCACTGATC ATGTGAGTGG TCAAATGCAC 
TTTAAAGTGG ATGTCACGTC TATGGGCCCA GATGATGTGT CTCCCGAGAC AATTCTGGGG 
GCAGCAGCCC TCAATGGAGC TCCTGGAACC CCTTCAGATG ACGAAGAGCT GCCTCATCCT 
CTACCCATCT CATCAGCAGG CCCTTCCCCA ACAGCCTCCC TGGGGTCCAA TAGAAATGGG 
GAGGGGAGCA GCATTCCCTC CCCCGACACA GAAATCTATG GTGGCACCCT GGACGACGAG 
GCCCCTTCTT CTCCAGACCT GCTCCAGGGG TCTTTCAGCG AGCAGCTTGA TGCCATCGAG 
GCTCCTAAGG GCCCAGGTGA CAGACCTCTA GGAGCAGCTT CTCCAAAACT CCGCTCCAGC 
TTTCCCACAC ATACGCGACT CAGTGCCATG CTGCACATCG ACTCAGATGA AGATGAGGAC 
AGATCTGCTG CTACAGAGGC CACGTTGTCA ACATTTAACG GCATTCCAAG GTCTTATCAA 
ACACCCCCAA AAGCAGCAGC AAGAGGAGCT GTGTTTGATG GAGGCATAGA GGAACCTTCA 
GAAGAGGCTG GGCTGGAGTC TGAAGTAGAA ACTCTGAATG CAGGAGAAGA GGTGCCTCCT 
GAGCCAGAGG TCGTGCCCAG TTCAGTCGCT CCCGAGGTGG AAACCGCCAG TCTGATGGAG 
AGGCAGGAGG AACAGGGCCG GGAGGTGGAG GTAGAGGAGG AGGAGGAAGA GGGCCTGGCG 
CGCAGTGAAG AGTTGGCAAC CGAAGTTGAT ATCTCCTCAA TGGCATCTGA CTGCTGCCCG 
GGGCCTGCAT CAGCATCTCA GGAGGCGGAG CAGGGGGCAG AGGCAGCCAT TGACCCAGGG 
GGAGAAGATC TCTCAGATGA TCCACCTACC ACTTACGAGG TTGCAGAGAC TGCTGAAGAA 
ACACATCTAA CTAATGAGCC GGAGAGAGAG GCTCCAAAAC CAGAGCCCCC CACCAACGAG 
GAAGAGGAAT GTGAAGATGT GACTGAGGAT ACCATGTCCA GAAGATGGAG CCTGGAGGCC 
ACAGCAGGAG TGTCACAGGA GGATGAAGAT GAGTTGATAC CATCCGGAGA TGGGGAATCT 
GTGGACTCTG AGACTTTTGT TACGGAGACA GAGTCAGAAT TAGGCGTTCC TCAGGTAAAC 
GGAGATCAGC GTGTTAATTC TCTGCCATCT GTAAGACAAG ACATCCACCG ATACCAGCGT 
GTGGACGAGC CCTTACCTCC CAACTGGGAG GCTCGTATTG ACAGTCATGG TCGTATCTTC 
TTTGTGGATC ACGTGAATCG CACCACCACA TGGCAGAGAC CCACTGGCCC TCCGGCTCCA 
CAGGGTCTGA CCCGCTCCAG CTCCATTCTG CAGATGGAAC AACTCAACCG CAGGTATCAG 
AGTATTCGCA GGACAATGAC CAGTGAAAGG TCTGAGGAAC CCAGAGTTGA AGATCCTCCA 
GCTGATGAAA CAGACGTGCT GGCTCAGTCC ATCAGTGAAT ACCGGAGGGA TGGCATTATT 
GGTCAGTCCA GCTCACGCTC TCGTCTGGCT CTTCTGCTTC AGTCTCCTGG TGCAAAGTTC 
CTGTCCAGTC CAGACTTCTT CTCTGTTCTG CATTCTAATC CTAGTGCCTA TCGGATGTTC 
ACAAGTAACA CGTGTCTGAA GCACATGATC AGTAAAGTTC GACGGGATGC CCAACACTTT 
GAGCGGTACC AGCACAACCG AGACCTGGTC AACTTCCTCA ATCTGTTCTC CAACAAGCAG 
CTGGAGCTTC CCCGGGGCTG GGAGATGAAA CATGACCATA CTGGGAAGGC CTTCTTTGTA 
GACCACAACT GTCGTGCCAC AACATTTATT GACCCGCGGC TGCCCTTGCA GAACTCGCGG 
TCCAGCGGAT TGTTGGCTCA CAGACAGCAC CTGTCCCGCC AGCGCAGCCA CAGTGCAGGA 
GAGGTTGGAG ATGACTCTCG TTCCCCGAGT CCTCCAGTCC AGGGCCGGTC ATCTAGAAAC 
ACCTATAATG ATAAGATTGT TGCCTTTTTG CGACAACCCA ACATTTTTGA GATCCTGCAA 
GAGAGACAGC CAGAACTTGT GAGAAACCAT TCCCTTAGGG AGAAGGTTCA GTTTATCCGC 
AATGAGGGGG CAACAGGGTT AACTCGTCTG TCCAGTGATG CAGACCTGGT TATGTTGCTC 
AGCCTGTTTG AAGAGGAAGT GATGTCGTAT GTTCCGCCCA ATGCCTTACT GCTCTCCAGC 
TACTGTCATG CGTCCCCACA GAGCTCCCCT GGAACCCCAC GGGCTAATGC GCGAGCCCCT 
GCTCCCTACA AGCGGGATTT TGAGGCCAAA TTAAGAAACT TCTACCGCAA AATGGAGACC 
AAGGGCTACG GACAGGGACC AGGAAAAGTG AAGTTAATAA TAAGAAGAGA TCACCTGCTT 
GAGGACGCTT TCAATCAGAT CATGTGTTAT TCCCGCAAAG ACCTTCAAAG GAGCAGGCTT 
TACGTCAGCT TTGTTGGAGA AGAAGGATTG GACTATAGTG GTCCATCCAG GGAGTTCTTC 
TTCCTGGTTT CCCGTGAGCT TTTTAACCCA TACTATGGAC TCTTTGAATA CTCAGCTAAT 
GACACTTACA CTGTGCAGAT CAGCCCTATG TCAGCCTTTG TGGACAACCA TCATGAGTGG 
TTCCGGTTCA GTGGCCGCAT TCTGGGTCTT GCTCTAATTC ATCAGTACCT GCTGGATGCC 
TTCTTCACCC GACCATTTTA CAAAGGCCTT CTCCGCATTC CATGTGATCT GAGTGATCTA 
GAGTTTCTGG ATGAAGAGTT TCATCAGAGT TTGCAGTGGA TGAAGGATAA TGACATCGAG 
GACATGCTGG ACCTCACCTT TACAGTCAAT GAGGAGGTGT TTGGACAGAT AACAGAACGT 
GAGCTAAAGC CGGGTGGATC TGGCATCGCT GTGTCAGACA AGAACAAGAA AGAGTACATT 
GAGCGCATGG TGAAGTGGCG GATTGAGAGA GGTGTGGCTC AGCAGACAGA GAGTCTTGTG 
CGAGGATTTT ATGAGGTGGT GGATGTGCGG CTCGTCTCAG TGTTTGATGC CCGAGAGCTG 
GAGCTGGTCA TTGCTGGAAC AGCAGAGATA GACCTGTCCG ACTGGAGAAA CAACACAGAA 
TACAGGGGAG GTTATCATGA CAACCACATA GTGATTCGAT GGTTCTGGGC TGCTGTGGAG 
AGATTCAACA ATGAACAGAG ACTACGACTC CTTCAGTTTG TAACAGGAAC TTCCAGCATT 
CCCTATGAGG GCTTTGCCTC TCTGCGGGGC AGTAATGGTC CACGCCGATT CTGTGTGGAG 
AAATGGGGCA AAGTGACCTC CCTCCCACGG GCTCACACCT GCTTTAACCG ACTGGATCTC 
CCTCCCTACC CCTCGTTCTC CATGCTCTAT GAGAAGATGG TCACGGCTGT GGAGGAAACC 
AGCACCTTTG GTCTGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334624.1
CDS	1072..5472
Translation	MAPQGRDHLATRESAGRRRSPNVRHTLSPENLRSLAERGGAEQAGVGLLRANSDTDLVSSQGRSSLTASTLEFTLCRGQNLVISWDIKEEVDATDWIGLYHIDETCPSNVWDCKNRGVNGTQCGQIVWRLEVGPYFLESETRICFKYYHGVSGALRATTPCITVKNPRASVDGQGEGQQASDSSRKLVSFTLSDIRAVGLKKGMFFNPDPYLKMSIHPGKRSCFPTFSHHGQERRSGITTNTTNPVWHGEKHTFVALMTDVLVIEVKDKFAKSRPIIKRFLGQLNIPVQLLLERHSTGNQSLSFSLCRRLPTDHVSGQMHFKVDVTSMGPDDVSPETILGAAALNGAPGTPSDDEELPHPLPISSAGPSPTASLGSNRNGEGSSIPSPDTEIYGGTLDDEAPSSPDLLQGSFSEQLDAIEAPKGPGDRPLGAASPKLRSSFPTHTRLSAMLHIDSDEDEDRSAATEATLSTFNGIPRSYQTPPKAAARGAVFDGGIEEPSEEAGLESEVETLNAGEEVPPEPEVVPSSVAPEVETASLMERQEEQGREVEVEEEEEEGLARSEELATEVDISSMASDCCPGPASASQEAEQGAEAAIDPGGEDLSDDPPTTYEVAETAEETHLTNEPEREAPKPEPPTNEEEECEDVTEDTMSRRWSLEATAGVSQEDEDELIPSGDGESVDSETFVTETESELGVPQVNGDQRVNSLPSVRQDIHRYQRVDEPLPPNWEARIDSHGRIFFVDHVNRTTTWQRPTGPPAPQGLTRSSSILQMEQLNRRYQSIRRTMTSERSEEPRVEDPPADETDVLAQSISEYRRDGIIGQSSSRSRLALLLQSPGAKFLSSPDFFSVLHSNPSAYRMFTSNTCLKHMISKVRRDAQHFERYQHNRDLVNFLNLFSNKQLELPRGWEMKHDHTGKAFFVDHNCRATTFIDPRLPLQNSRSSGLLAHRQHLSRQRSHSAGEVGDDSRSPSPPVQGRSSRNTYNDKIVAFLRQPNIFEILQERQPELVRNHSLREKVQFIRNEGATGLTRLSSDADLVMLLSLFEEEVMSYVPPNALLLSSYCHASPQSSPGTPRANARAPAPYKRDFEAKLRNFYRKMETKGYGQGPGKVKLIIRRDHLLEDAFNQIMCYSRKDLQRSRLYVSFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLRIPCDLSDLEFLDEEFHQSLQWMKDNDIEDMLDLTFTVNEEVFGQITERELKPGGSGIAVSDKNKKEYIERMVKWRIERGVAQQTESLVRGFYEVVDVRLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKVTSLPRAHTCFNRLDLPPYPSFSMLYEKMVTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_021478949.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478949.1

ORF Insert Sequence:

ATGGCGCCTC AGGGGCGGGA TCACCTGGCC ACACGGGAGT CTGCAGGCCG AAGACGCAGC 
CCGAATGTGC GGCACACGCT CAGCCCGGAA AACCTGCGAA GCCTGGCGGA GCGCGGTGGA 
GCTGAACAAG CGGGAGTAGG ACTGCTCAGA GCCAATAGCG ACACAGACCT GGTCAGTTCT 
CAAGGGCGAT CCTCACTCAC AGCCTCCACT CTAGAGTTTA CACTGTGCCG CGGACAGAAC 
CTCGTCATCT CATGGGACAT CAAGGAGGAG GTGGACGCCA CTGACTGGAT CGGCCTGTAC 
CACATAGATG AAACCTGTCC ATCAAATGTG TGGGACTGTA AGAACCGTGG GGTAAACGGC 
ACACAGTGTG GTCAGATCGT CTGGAGATTA GAGGTCGGGC CATACTTTCT TGAATCTGAG 
ACAAGGATCT GCTTTAAATA CTACCATGGA GTAAGTGGAG CATTGAGAGC CACGACTCCC 
TGCATCACAG TCAAGAACCC CAGAGCCTCG GTTGATGGCC AAGGAGAAGG ACAACAAGCT 
TCAGACAGTA GTCGAAAACT GGTTAGCTTT ACGCTATCAG ATATCCGAGC TGTTGGCCTG 
AAGAAGGGGA TGTTTTTTAA CCCAGACCCT TATCTGAAGA TGAGCATTCA TCCAGGCAAA 
AGAAGCTGCT TTCCGACCTT TTCCCATCAC GGCCAAGAGA GGCGATCCGG CATTACAACT 
AACACCACTA ATCCTGTTTG GCATGGAGAG AAACATACTT TTGTGGCTCT TATGACAGAT 
GTATTAGTGA TTGAGGTTAA AGACAAGTTT GCTAAAAGTC GACCCATTAT TAAACGCTTC 
TTGGGTCAGC TGAATATTCC TGTGCAGCTG CTGCTGGAGA GACATTCGAC AGGGAACCAG 
TCACTCAGCT TCTCCCTATG TCGTCGTTTG CCCACTGATC ATGTGAGTGG TCAAATGCAC 
TTTAAAGTGG ATGTCACGTC TATGGGCCCA GATGATGTGT CTCCCGAGAC AATTCTGGGG 
GCAGCAGCCC TCAATGGAGC TCCTGGAACC CCTTCAGATG ACGAAGAGCT GCCTCATCCT 
CTACCCATCT CATCAGCAGG CCCTTCCCCA ACAGCCTCCC TGGGGTCCAA TAGAAATGGG 
GAGGGGAGCA GCATTCCCTC CCCCGACACA GAAATCTATG GTGGCACCCT GGACGACGAG 
GCCCCTTCTT CTCCAGACCT GCTCCAGGGG TCTTTCAGCG AGCAGCTTGA TGCCATCGAG 
GCTCCTAAGG GCCCAGGTGA CAGACCTCTA GGAGCAGCTT CTCCAAAACT CCGCTCCAGC 
TTTCCCACAC ATACGCGACT CAGTGCCATG CTGCACATCG ACTCAGATGA AGATGAGGAC 
AGATCTGCTG CTACAGAGGC CACGTTGTCA ACATTTAACG GCATTCCAAG GTCTTATCAA 
ACACCCCCAA AAGCAGCAGC AAGAGGAGCT GTGTTTGATG GAGGCATAGA GGAACCTTCA 
GAAGAGGCTG GGCTGGAGTC TGAAGTAGAA ACTCTGAATG CAGGAGAAGA GGTGCCTCCT 
GAGCCAGAGG TCGTGCCCAG TTCAGTCGCT CCCGAGGTGG AAACCGCCAG TCTGATGGAG 
AGGCAGGAGG AACAGGGCCG GGAGGTGGAG GTAGAGGAGG AGGAGGAAGA GGGCCTGGCG 
CGCAGTGAAG AGTTGGCAAC CGAAGTTGAT ATCTCCTCAA TGGCATCTGA CTGCTGCCCG 
GGGCCTGCAT CAGCATCTCA GGAGGCGGAG CAGGGGGCAG AGGCAGCCAT TGACCCAGGG 
GGAGAAGATC TCTCAGATGA TCCACCTACC ACTTACGAGG TTGCAGAGAC TGCTGAAGAA 
ACACATCTAA CTAATGAGCC GGAGAGAGAG GCTCCAAAAC CAGAGCCCCC CACCAACGAG 
GAAGAGGAAT GTGAAGATGT GACTGAGGAT ACCATGTCCA GAAGATGGAG CCTGGAGGCC 
ACAGCAGGAG TGTCACAGGA GGATGAAGAT GAGTTGATAC CATCCGGAGA TGGGGAATCT 
GTGGACTCTG AGACTTTTGT TACGGAGACA GAGTCAGAAT TAGGCGTTCC TCAGGTAAAC 
GGAGATCAGC GTGTTAATTC TCTGCCATCT GTAAGACAAG ACATCCACCG ATACCAGCGT 
GTGGACGAGC CCTTACCTCC CAACTGGGAG GCTCGTATTG ACAGTCATGG TCGTATCTTC 
TTTGTGGATC ACGTGAATCG CACCACCACA TGGCAGAGAC CCACTGGCCC TCCGGCTCCA 
CAGGGTCTGA CCCGCTCCAG CTCCATTCTG CAGATGGAAC AACTCAACCG CAGGTATCAG 
AGTATTCGCA GGACAATGAC CAGTGAAAGG TCTGAGGAAC CCAGAGTTGA AGATCCTCCA 
GCTGATGAAA CAGACGTGCT GGCTCAGTCC ATCAGTGAAT ACCGGAGGGA TGGCATTATT 
GGTCAGTCCA GCTCACGCTC TCGTCTGGCT CTTCTGCTTC AGTCTCCTGG TGCAAAGTTC 
CTGTCCAGTC CAGACTTCTT CTCTGTTCTG CATTCTAATC CTAGTGCCTA TCGGATGTTC 
ACAAGTAACA CGTGTCTGAA GCACATGATC AGTAAAGTTC GACGGGATGC CCAACACTTT 
GAGCGGTACC AGCACAACCG AGACCTGGTC AACTTCCTCA ATCTGTTCTC CAACAAGCAG 
CTGGAGCTTC CCCGGGGCTG GGAGATGAAA CATGACCATA CTGGGAAGGC CTTCTTTGTA 
GACCACAACT GTCGTGCCAC AACATTTATT GACCCGCGGC TGCCCTTGCA GAACTCGCGG 
TCCAGCGGAT TGTTGGCTCA CAGACAGCAC CTGTCCCGCC AGCGCAGCCA CAGTGCAGGA 
GAGGTTGGAG ATGACTCTCG TTCCCCGAGT CCTCCAGTCC AGGGCCGGTC ATCTAGAAAC 
ACCTATAATG ATAAGATTGT TGCCTTTTTG CGACAACCCA ACATTTTTGA GATCCTGCAA 
GAGAGACAGC CAGAACTTGT GAGAAACCAT TCCCTTAGGG AGAAGGTTCA GTTTATCCGC 
AATGAGGGGG CAACAGGGTT AACTCGTCTG TCCAGTGATG CAGACCTGGT TATGTTGCTC 
AGCCTGTTTG AAGAGGAAGT GATGTCGTAT GTTCCGCCCA ATGCCTTACT GCTCTCCAGC 
TACTGTCATG CGTCCCCACA GAGCTCCCCT GGAACCCCAC GGGCTAATGC GCGAGCCCCT 
GCTCCCTACA AGCGGGATTT TGAGGCCAAA TTAAGAAACT TCTACCGCAA AATGGAGACC 
AAGGGCTACG GACAGGGACC AGGAAAAGTG AAGTTAATAA TAAGAAGAGA TCACCTGCTT 
GAGGACGCTT TCAATCAGAT CATGTGTTAT TCCCGCAAAG ACCTTCAAAG GAGCAGGCTT 
TACGTCAGCT TTGTTGGAGA AGAAGGATTG GACTATAGTG GTCCATCCAG GGAGTTCTTC 
TTCCTGGTTT CCCGTGAGCT TTTTAACCCA TACTATGGAC TCTTTGAATA CTCAGCTAAT 
GACACTTACA CTGTGCAGAT CAGCCCTATG TCAGCCTTTG TGGACAACCA TCATGAGTGG 
TTCCGGTTCA GTGGCCGCAT TCTGGGTCTT GCTCTAATTC ATCAGTACCT GCTGGATGCC 
TTCTTCACCC GACCATTTTA CAAAGGCCTT CTCCGCATTC CATGTGATCT GAGTGATCTA 
GAGTTTCTGG ATGAAGAGTT TCATCAGAGT TTGCAGTGGA TGAAGGATAA TGACATCGAG 
GACATGCTGG ACCTCACCTT TACAGTCAAT GAGGAGGTGT TTGGACAGAT AACAGAACGT 
GAGCTAAAGC CGGGTGGATC TGGCATCGCT GTGTCAGACA AGAACAAGAA AGAGTACATT 
GAGCGCATGG TGAAGTGGCG GATTGAGAGA GGTGTGGCTC AGCAGACAGA GAGTCTTGTG 
CGAGGATTTT ATGAGGTGGT GGATGTGCGG CTCGTCTCAG TGTTTGATGC CCGAGAGCTG 
GAGCTGGTCA TTGCTGGAAC AGCAGAGATA GACCTGTCCG ACTGGAGAAA CAACACAGAA 
TACAGGGGAG GTTATCATGA CAACCACATA GTGATTCGAT GGTTCTGGGC TGCTGTGGAG 
AGATTCAACA ATGAACAGAG ACTACGACTC CTTCAGTTTG TAACAGGAAC TTCCAGCATT 
CCCTATGAGG GCTTTGCCTC TCTGCGGGGC AGTAATGGTC CACGCCGATT CTGTGTGGAG 
AAATGGGGCA AAGTGACCTC CCTCCCACGG GCTCACACCT GCTTTAACCG ACTGGATCTC 
CCTCCCTACC CCTCGTTCTC CATGCTCTAT GAGAAGATGG TCACGGCTGT GGAGGAAACC 
AGCACCTTTG GTCTGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2A ( XM_009304702.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302977.1 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa48866
Clone ID Related Accession (Same CDS sequence)	XM_021478949.1 , XM_009304702.3 , XM_009304702.2
Accession Version	XM_009304702.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase HECW2-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394753.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304702.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCTC AGGGGCGGGA TCACCTGGCC ACACGGGAGT CTGCAGGCCG AAGACGCAGC 
CCGAATGTGC GGCACACGCT CAGCCCGGAA AACCTGCGAA GCCTGGCGGA GCGCGGTGGA 
GCTGAACAAG CGGGAGTAGG ACTGCTCAGA GCCAATAGCG ACACAGACCT GGTCAGTTCT 
CAAGGGCGAT CCTCACTCAC AGCCTCCACT CTAGAGTTTA CACTGTGCCG CGGACAGAAC 
CTCGTCATCT CATGGGACAT CAAGGAGGAG GTGGACGCCA CTGACTGGAT CGGCCTGTAC 
CACATAGATG AAACCTGTCC ATCAAATGTG TGGGACTGTA AGAACCGTGG GGTAAACGGC 
ACACAGTGTG GTCAGATCGT CTGGAGATTA GAGGTCGGGC CATACTTTCT TGAATCTGAG 
ACAAGGATCT GCTTTAAATA CTACCATGGA GTAAGTGGAG CATTGAGAGC CACGACTCCC 
TGCATCACAG TCAAGAACCC CAGAGCCTCG GTTGATGGCC AAGGAGAAGG ACAACAAGCT 
TCAGACAGTA GTCGAAAACT GGTTAGCTTT ACGCTATCAG ATATCCGAGC TGTTGGCCTG 
AAGAAGGGGA TGTTTTTTAA CCCAGACCCT TATCTGAAGA TGAGCATTCA TCCAGGCAAA 
AGAAGCTGCT TTCCGACCTT TTCCCATCAC GGCCAAGAGA GGCGATCCGG CATTACAACT 
AACACCACTA ATCCTGTTTG GCATGGAGAG AAACATACTT TTGTGGCTCT TATGACAGAT 
GTATTAGTGA TTGAGGTTAA AGACAAGTTT GCTAAAAGTC GACCCATTAT TAAACGCTTC 
TTGGGTCAGC TGAATATTCC TGTGCAGCTG CTGCTGGAGA GACATTCGAC AGGGAACCAG 
TCACTCAGCT TCTCCCTATG TCGTCGTTTG CCCACTGATC ATGTGAGTGG TCAAATGCAC 
TTTAAAGTGG ATGTCACGTC TATGGGCCCA GATGATGTGT CTCCCGAGAC AATTCTGGGG 
GCAGCAGCCC TCAATGGAGC TCCTGGAACC CCTTCAGATG ACGAAGAGCT GCCTCATCCT 
CTACCCATCT CATCAGCAGG CCCTTCCCCA ACAGCCTCCC TGGGGTCCAA TAGAAATGGG 
GAGGGGAGCA GCATTCCCTC CCCCGACACA GAAATCTATG GTGGCACCCT GGACGACGAG 
GCCCCTTCTT CTCCAGACCT GCTCCAGGGG TCTTTCAGCG AGCAGCTTGA TGCCATCGAG 
GCTCCTAAGG GCCCAGGTGA CAGACCTCTA GGAGCAGCTT CTCCAAAACT CCGCTCCAGC 
TTTCCCACAC ATACGCGACT CAGTGCCATG CTGCACATCG ACTCAGATGA AGATGAGGAC 
AGATCTGCTG CTACAGAGGC CACGTTGTCA ACATTTAACG GCATTCCAAG GTCTTATCAA 
ACACCCCCAA AAGCAGCAGC AAGAGGAGCT GTGTTTGATG GAGGCATAGA GGAACCTTCA 
GAAGAGGCTG GGCTGGAGTC TGAAGTAGAA ACTCTGAATG CAGGAGAAGA GGTGCCTCCT 
GAGCCAGAGG TCGTGCCCAG TTCAGTCGCT CCCGAGGTGG AAACCGCCAG TCTGATGGAG 
AGGCAGGAGG AACAGGGCCG GGAGGTGGAG GTAGAGGAGG AGGAGGAAGA GGGCCTGGCG 
CGCAGTGAAG AGTTGGCAAC CGAAGTTGAT ATCTCCTCAA TGGCATCTGA CTGCTGCCCG 
GGGCCTGCAT CAGCATCTCA GGAGGCGGAG CAGGGGGCAG AGGCAGCCAT TGACCCAGGG 
GGAGAAGATC TCTCAGATGA TCCACCTACC ACTTACGAGG TTGCAGAGAC TGCTGAAGAA 
ACACATCTAA CTAATGAGCC GGAGAGAGAG GCTCCAAAAC CAGAGCCCCC CACCAACGAG 
GAAGAGGAAT GTGAAGATGT GACTGAGGAT ACCATGTCCA GAAGATGGAG CCTGGAGGCC 
ACAGCAGGAG TGTCACAGGA GGATGAAGAT GAGTTGATAC CATCCGGAGA TGGGGAATCT 
GTGGACTCTG AGACTTTTGT TACGGAGACA GAGTCAGAAT TAGGCGTTCC TCAGGTAAAC 
GGAGATCAGC GTGTTAATTC TCTGCCATCT GTAAGACAAG ACATCCACCG ATACCAGCGT 
GTGGACGAGC CCTTACCTCC CAACTGGGAG GCTCGTATTG ACAGTCATGG TCGTATCTTC 
TTTGTGGATC ACGTGAATCG CACCACCACA TGGCAGAGAC CCACTGGCCC TCCGGCTCCA 
CAGGGTCTGA CCCGCTCCAG CTCCATTCTG CAGATGGAAC AACTCAACCG CAGGTATCAG 
AGTATTCGCA GGACAATGAC CAGTGAAAGG TCTGAGGAAC CCAGAGTTGA AGATCCTCCA 
GCTGATGAAA CAGACGTGCT GGCTCAGTCC ATCAGTGAAT ACCGGAGGGA TGGCATTATT 
GGTCAGTCCA GCTCACGCTC TCGTCTGGCT CTTCTGCTTC AGTCTCCTGG TGCAAAGTTC 
CTGTCCAGTC CAGACTTCTT CTCTGTTCTG CATTCTAATC CTAGTGCCTA TCGGATGTTC 
ACAAGTAACA CGTGTCTGAA GCACATGATC AGTAAAGTTC GACGGGATGC CCAACACTTT 
GAGCGGTACC AGCACAACCG AGACCTGGTC AACTTCCTCA ATCTGTTCTC CAACAAGCAG 
CTGGAGCTTC CCCGGGGCTG GGAGATGAAA CATGACCATA CTGGGAAGGC CTTCTTTGTA 
GACCACAACT GTCGTGCCAC AACATTTATT GACCCGCGGC TGCCCTTGCA GAACTCGCGG 
TCCAGCGGAT TGTTGGCTCA CAGACAGCAC CTGTCCCGCC AGCGCAGCCA CAGTGCAGGA 
GAGGTTGGAG ATGACTCTCG TTCCCCGAGT CCTCCAGTCC AGGGCCGGTC ATCTAGAAAC 
ACCTATAATG ATAAGATTGT TGCCTTTTTG CGACAACCCA ACATTTTTGA GATCCTGCAA 
GAGAGACAGC CAGAACTTGT GAGAAACCAT TCCCTTAGGG AGAAGGTTCA GTTTATCCGC 
AATGAGGGGG CAACAGGGTT AACTCGTCTG TCCAGTGATG CAGACCTGGT TATGTTGCTC 
AGCCTGTTTG AAGAGGAAGT GATGTCGTAT GTTCCGCCCA ATGCCTTACT GCTCTCCAGC 
TACTGTCATG CGTCCCCACA GAGCTCCCCT GGAACCCCAC GGGCTAATGC GCGAGCCCCT 
GCTCCCTACA AGCGGGATTT TGAGGCCAAA TTAAGAAACT TCTACCGCAA AATGGAGACC 
AAGGGCTACG GACAGGGACC AGGAAAAGTG AAGTTAATAA TAAGAAGAGA TCACCTGCTT 
GAGGACGCTT TCAATCAGAT CATGTGTTAT TCCCGCAAAG ACCTTCAAAG GAGCAGGCTT 
TACGTCAGCT TTGTTGGAGA AGAAGGATTG GACTATAGTG GTCCATCCAG GGAGTTCTTC 
TTCCTGGTTT CCCGTGAGCT TTTTAACCCA TACTATGGAC TCTTTGAATA CTCAGCTAAT 
GACACTTACA CTGTGCAGAT CAGCCCTATG TCAGCCTTTG TGGACAACCA TCATGAGTGG 
TTCCGGTTCA GTGGCCGCAT TCTGGGTCTT GCTCTAATTC ATCAGTACCT GCTGGATGCC 
TTCTTCACCC GACCATTTTA CAAAGGCCTT CTCCGCATTC CATGTGATCT GAGTGATCTA 
GAGTTTCTGG ATGAAGAGTT TCATCAGAGT TTGCAGTGGA TGAAGGATAA TGACATCGAG 
GACATGCTGG ACCTCACCTT TACAGTCAAT GAGGAGGTGT TTGGACAGAT AACAGAACGT 
GAGCTAAAGC CGGGTGGATC TGGCATCGCT GTGTCAGACA AGAACAAGAA AGAGTACATT 
GAGCGCATGG TGAAGTGGCG GATTGAGAGA GGTGTGGCTC AGCAGACAGA GAGTCTTGTG 
CGAGGATTTT ATGAGGTGGT GGATGTGCGG CTCGTCTCAG TGTTTGATGC CCGAGAGCTG 
GAGCTGGTCA TTGCTGGAAC AGCAGAGATA GACCTGTCCG ACTGGAGAAA CAACACAGAA 
TACAGGGGAG GTTATCATGA CAACCACATA GTGATTCGAT GGTTCTGGGC TGCTGTGGAG 
AGATTCAACA ATGAACAGAG ACTACGACTC CTTCAGTTTG TAACAGGAAC TTCCAGCATT 
CCCTATGAGG GCTTTGCCTC TCTGCGGGGC AGTAATGGTC CACGCCGATT CTGTGTGGAG 
AAATGGGGCA AAGTGACCTC CCTCCCACGG GCTCACACCT GCTTTAACCG ACTGGATCTC 
CCTCCCTACC CCTCGTTCTC CATGCTCTAT GAGAAGATGG TCACGGCTGT GGAGGAAACC 
AGCACCTTTG GTCTGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302977.1
CDS	1072..5472
Translation	MAPQGRDHLATRESAGRRRSPNVRHTLSPENLRSLAERGGAEQAGVGLLRANSDTDLVSSQGRSSLTASTLEFTLCRGQNLVISWDIKEEVDATDWIGLYHIDETCPSNVWDCKNRGVNGTQCGQIVWRLEVGPYFLESETRICFKYYHGVSGALRATTPCITVKNPRASVDGQGEGQQASDSSRKLVSFTLSDIRAVGLKKGMFFNPDPYLKMSIHPGKRSCFPTFSHHGQERRSGITTNTTNPVWHGEKHTFVALMTDVLVIEVKDKFAKSRPIIKRFLGQLNIPVQLLLERHSTGNQSLSFSLCRRLPTDHVSGQMHFKVDVTSMGPDDVSPETILGAAALNGAPGTPSDDEELPHPLPISSAGPSPTASLGSNRNGEGSSIPSPDTEIYGGTLDDEAPSSPDLLQGSFSEQLDAIEAPKGPGDRPLGAASPKLRSSFPTHTRLSAMLHIDSDEDEDRSAATEATLSTFNGIPRSYQTPPKAAARGAVFDGGIEEPSEEAGLESEVETLNAGEEVPPEPEVVPSSVAPEVETASLMERQEEQGREVEVEEEEEEGLARSEELATEVDISSMASDCCPGPASASQEAEQGAEAAIDPGGEDLSDDPPTTYEVAETAEETHLTNEPEREAPKPEPPTNEEEECEDVTEDTMSRRWSLEATAGVSQEDEDELIPSGDGESVDSETFVTETESELGVPQVNGDQRVNSLPSVRQDIHRYQRVDEPLPPNWEARIDSHGRIFFVDHVNRTTTWQRPTGPPAPQGLTRSSSILQMEQLNRRYQSIRRTMTSERSEEPRVEDPPADETDVLAQSISEYRRDGIIGQSSSRSRLALLLQSPGAKFLSSPDFFSVLHSNPSAYRMFTSNTCLKHMISKVRRDAQHFERYQHNRDLVNFLNLFSNKQLELPRGWEMKHDHTGKAFFVDHNCRATTFIDPRLPLQNSRSSGLLAHRQHLSRQRSHSAGEVGDDSRSPSPPVQGRSSRNTYNDKIVAFLRQPNIFEILQERQPELVRNHSLREKVQFIRNEGATGLTRLSSDADLVMLLSLFEEEVMSYVPPNALLLSSYCHASPQSSPGTPRANARAPAPYKRDFEAKLRNFYRKMETKGYGQGPGKVKLIIRRDHLLEDAFNQIMCYSRKDLQRSRLYVSFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLRIPCDLSDLEFLDEEFHQSLQWMKDNDIEDMLDLTFTVNEEVFGQITERELKPGGSGIAVSDKNKKEYIERMVKWRIERGVAQQTESLVRGFYEVVDVRLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKVTSLPRAHTCFNRLDLPPYPSFSMLYEKMVTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009304702.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304702.3

ORF Insert Sequence:

ATGGCGCCTC AGGGGCGGGA TCACCTGGCC ACACGGGAGT CTGCAGGCCG AAGACGCAGC 
CCGAATGTGC GGCACACGCT CAGCCCGGAA AACCTGCGAA GCCTGGCGGA GCGCGGTGGA 
GCTGAACAAG CGGGAGTAGG ACTGCTCAGA GCCAATAGCG ACACAGACCT GGTCAGTTCT 
CAAGGGCGAT CCTCACTCAC AGCCTCCACT CTAGAGTTTA CACTGTGCCG CGGACAGAAC 
CTCGTCATCT CATGGGACAT CAAGGAGGAG GTGGACGCCA CTGACTGGAT CGGCCTGTAC 
CACATAGATG AAACCTGTCC ATCAAATGTG TGGGACTGTA AGAACCGTGG GGTAAACGGC 
ACACAGTGTG GTCAGATCGT CTGGAGATTA GAGGTCGGGC CATACTTTCT TGAATCTGAG 
ACAAGGATCT GCTTTAAATA CTACCATGGA GTAAGTGGAG CATTGAGAGC CACGACTCCC 
TGCATCACAG TCAAGAACCC CAGAGCCTCG GTTGATGGCC AAGGAGAAGG ACAACAAGCT 
TCAGACAGTA GTCGAAAACT GGTTAGCTTT ACGCTATCAG ATATCCGAGC TGTTGGCCTG 
AAGAAGGGGA TGTTTTTTAA CCCAGACCCT TATCTGAAGA TGAGCATTCA TCCAGGCAAA 
AGAAGCTGCT TTCCGACCTT TTCCCATCAC GGCCAAGAGA GGCGATCCGG CATTACAACT 
AACACCACTA ATCCTGTTTG GCATGGAGAG AAACATACTT TTGTGGCTCT TATGACAGAT 
GTATTAGTGA TTGAGGTTAA AGACAAGTTT GCTAAAAGTC GACCCATTAT TAAACGCTTC 
TTGGGTCAGC TGAATATTCC TGTGCAGCTG CTGCTGGAGA GACATTCGAC AGGGAACCAG 
TCACTCAGCT TCTCCCTATG TCGTCGTTTG CCCACTGATC ATGTGAGTGG TCAAATGCAC 
TTTAAAGTGG ATGTCACGTC TATGGGCCCA GATGATGTGT CTCCCGAGAC AATTCTGGGG 
GCAGCAGCCC TCAATGGAGC TCCTGGAACC CCTTCAGATG ACGAAGAGCT GCCTCATCCT 
CTACCCATCT CATCAGCAGG CCCTTCCCCA ACAGCCTCCC TGGGGTCCAA TAGAAATGGG 
GAGGGGAGCA GCATTCCCTC CCCCGACACA GAAATCTATG GTGGCACCCT GGACGACGAG 
GCCCCTTCTT CTCCAGACCT GCTCCAGGGG TCTTTCAGCG AGCAGCTTGA TGCCATCGAG 
GCTCCTAAGG GCCCAGGTGA CAGACCTCTA GGAGCAGCTT CTCCAAAACT CCGCTCCAGC 
TTTCCCACAC ATACGCGACT CAGTGCCATG CTGCACATCG ACTCAGATGA AGATGAGGAC 
AGATCTGCTG CTACAGAGGC CACGTTGTCA ACATTTAACG GCATTCCAAG GTCTTATCAA 
ACACCCCCAA AAGCAGCAGC AAGAGGAGCT GTGTTTGATG GAGGCATAGA GGAACCTTCA 
GAAGAGGCTG GGCTGGAGTC TGAAGTAGAA ACTCTGAATG CAGGAGAAGA GGTGCCTCCT 
GAGCCAGAGG TCGTGCCCAG TTCAGTCGCT CCCGAGGTGG AAACCGCCAG TCTGATGGAG 
AGGCAGGAGG AACAGGGCCG GGAGGTGGAG GTAGAGGAGG AGGAGGAAGA GGGCCTGGCG 
CGCAGTGAAG AGTTGGCAAC CGAAGTTGAT ATCTCCTCAA TGGCATCTGA CTGCTGCCCG 
GGGCCTGCAT CAGCATCTCA GGAGGCGGAG CAGGGGGCAG AGGCAGCCAT TGACCCAGGG 
GGAGAAGATC TCTCAGATGA TCCACCTACC ACTTACGAGG TTGCAGAGAC TGCTGAAGAA 
ACACATCTAA CTAATGAGCC GGAGAGAGAG GCTCCAAAAC CAGAGCCCCC CACCAACGAG 
GAAGAGGAAT GTGAAGATGT GACTGAGGAT ACCATGTCCA GAAGATGGAG CCTGGAGGCC 
ACAGCAGGAG TGTCACAGGA GGATGAAGAT GAGTTGATAC CATCCGGAGA TGGGGAATCT 
GTGGACTCTG AGACTTTTGT TACGGAGACA GAGTCAGAAT TAGGCGTTCC TCAGGTAAAC 
GGAGATCAGC GTGTTAATTC TCTGCCATCT GTAAGACAAG ACATCCACCG ATACCAGCGT 
GTGGACGAGC CCTTACCTCC CAACTGGGAG GCTCGTATTG ACAGTCATGG TCGTATCTTC 
TTTGTGGATC ACGTGAATCG CACCACCACA TGGCAGAGAC CCACTGGCCC TCCGGCTCCA 
CAGGGTCTGA CCCGCTCCAG CTCCATTCTG CAGATGGAAC AACTCAACCG CAGGTATCAG 
AGTATTCGCA GGACAATGAC CAGTGAAAGG TCTGAGGAAC CCAGAGTTGA AGATCCTCCA 
GCTGATGAAA CAGACGTGCT GGCTCAGTCC ATCAGTGAAT ACCGGAGGGA TGGCATTATT 
GGTCAGTCCA GCTCACGCTC TCGTCTGGCT CTTCTGCTTC AGTCTCCTGG TGCAAAGTTC 
CTGTCCAGTC CAGACTTCTT CTCTGTTCTG CATTCTAATC CTAGTGCCTA TCGGATGTTC 
ACAAGTAACA CGTGTCTGAA GCACATGATC AGTAAAGTTC GACGGGATGC CCAACACTTT 
GAGCGGTACC AGCACAACCG AGACCTGGTC AACTTCCTCA ATCTGTTCTC CAACAAGCAG 
CTGGAGCTTC CCCGGGGCTG GGAGATGAAA CATGACCATA CTGGGAAGGC CTTCTTTGTA 
GACCACAACT GTCGTGCCAC AACATTTATT GACCCGCGGC TGCCCTTGCA GAACTCGCGG 
TCCAGCGGAT TGTTGGCTCA CAGACAGCAC CTGTCCCGCC AGCGCAGCCA CAGTGCAGGA 
GAGGTTGGAG ATGACTCTCG TTCCCCGAGT CCTCCAGTCC AGGGCCGGTC ATCTAGAAAC 
ACCTATAATG ATAAGATTGT TGCCTTTTTG CGACAACCCA ACATTTTTGA GATCCTGCAA 
GAGAGACAGC CAGAACTTGT GAGAAACCAT TCCCTTAGGG AGAAGGTTCA GTTTATCCGC 
AATGAGGGGG CAACAGGGTT AACTCGTCTG TCCAGTGATG CAGACCTGGT TATGTTGCTC 
AGCCTGTTTG AAGAGGAAGT GATGTCGTAT GTTCCGCCCA ATGCCTTACT GCTCTCCAGC 
TACTGTCATG CGTCCCCACA GAGCTCCCCT GGAACCCCAC GGGCTAATGC GCGAGCCCCT 
GCTCCCTACA AGCGGGATTT TGAGGCCAAA TTAAGAAACT TCTACCGCAA AATGGAGACC 
AAGGGCTACG GACAGGGACC AGGAAAAGTG AAGTTAATAA TAAGAAGAGA TCACCTGCTT 
GAGGACGCTT TCAATCAGAT CATGTGTTAT TCCCGCAAAG ACCTTCAAAG GAGCAGGCTT 
TACGTCAGCT TTGTTGGAGA AGAAGGATTG GACTATAGTG GTCCATCCAG GGAGTTCTTC 
TTCCTGGTTT CCCGTGAGCT TTTTAACCCA TACTATGGAC TCTTTGAATA CTCAGCTAAT 
GACACTTACA CTGTGCAGAT CAGCCCTATG TCAGCCTTTG TGGACAACCA TCATGAGTGG 
TTCCGGTTCA GTGGCCGCAT TCTGGGTCTT GCTCTAATTC ATCAGTACCT GCTGGATGCC 
TTCTTCACCC GACCATTTTA CAAAGGCCTT CTCCGCATTC CATGTGATCT GAGTGATCTA 
GAGTTTCTGG ATGAAGAGTT TCATCAGAGT TTGCAGTGGA TGAAGGATAA TGACATCGAG 
GACATGCTGG ACCTCACCTT TACAGTCAAT GAGGAGGTGT TTGGACAGAT AACAGAACGT 
GAGCTAAAGC CGGGTGGATC TGGCATCGCT GTGTCAGACA AGAACAAGAA AGAGTACATT 
GAGCGCATGG TGAAGTGGCG GATTGAGAGA GGTGTGGCTC AGCAGACAGA GAGTCTTGTG 
CGAGGATTTT ATGAGGTGGT GGATGTGCGG CTCGTCTCAG TGTTTGATGC CCGAGAGCTG 
GAGCTGGTCA TTGCTGGAAC AGCAGAGATA GACCTGTCCG ACTGGAGAAA CAACACAGAA 
TACAGGGGAG GTTATCATGA CAACCACATA GTGATTCGAT GGTTCTGGGC TGCTGTGGAG 
AGATTCAACA ATGAACAGAG ACTACGACTC CTTCAGTTTG TAACAGGAAC TTCCAGCATT 
CCCTATGAGG GCTTTGCCTC TCTGCGGGGC AGTAATGGTC CACGCCGATT CTGTGTGGAG 
AAATGGGGCA AAGTGACCTC CCTCCCACGG GCTCACACCT GCTTTAACCG ACTGGATCTC 
CCTCCCTACC CCTCGTTCTC CATGCTCTAT GAGAAGATGG TCACGGCTGT GGAGGAAACC 
AGCACCTTTG GTCTGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2A ( XM_009304702.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302977.1 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa48866
Clone ID Related Accession (Same CDS sequence)	XM_021478949.1 , XM_009304702.3 , XM_009304702.2
Accession Version	XM_009304702.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase HECW2-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304702.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCTC AGGGGCGGGA TCACCTGGCC ACACGGGAGT CTGCAGGCCG AAGACGCAGC 
CCGAATGTGC GGCACACGCT CAGCCCGGAA AACCTGCGAA GCCTGGCGGA GCGCGGTGGA 
GCTGAACAAG CGGGAGTAGG ACTGCTCAGA GCCAATAGCG ACACAGACCT GGTCAGTTCT 
CAAGGGCGAT CCTCACTCAC AGCCTCCACT CTAGAGTTTA CACTGTGCCG CGGACAGAAC 
CTCGTCATCT CATGGGACAT CAAGGAGGAG GTGGACGCCA CTGACTGGAT CGGCCTGTAC 
CACATAGATG AAACCTGTCC ATCAAATGTG TGGGACTGTA AGAACCGTGG GGTAAACGGC 
ACACAGTGTG GTCAGATCGT CTGGAGATTA GAGGTCGGGC CATACTTTCT TGAATCTGAG 
ACAAGGATCT GCTTTAAATA CTACCATGGA GTAAGTGGAG CATTGAGAGC CACGACTCCC 
TGCATCACAG TCAAGAACCC CAGAGCCTCG GTTGATGGCC AAGGAGAAGG ACAACAAGCT 
TCAGACAGTA GTCGAAAACT GGTTAGCTTT ACGCTATCAG ATATCCGAGC TGTTGGCCTG 
AAGAAGGGGA TGTTTTTTAA CCCAGACCCT TATCTGAAGA TGAGCATTCA TCCAGGCAAA 
AGAAGCTGCT TTCCGACCTT TTCCCATCAC GGCCAAGAGA GGCGATCCGG CATTACAACT 
AACACCACTA ATCCTGTTTG GCATGGAGAG AAACATACTT TTGTGGCTCT TATGACAGAT 
GTATTAGTGA TTGAGGTTAA AGACAAGTTT GCTAAAAGTC GACCCATTAT TAAACGCTTC 
TTGGGTCAGC TGAATATTCC TGTGCAGCTG CTGCTGGAGA GACATTCGAC AGGGAACCAG 
TCACTCAGCT TCTCCCTATG TCGTCGTTTG CCCACTGATC ATGTGAGTGG TCAAATGCAC 
TTTAAAGTGG ATGTCACGTC TATGGGCCCA GATGATGTGT CTCCCGAGAC AATTCTGGGG 
GCAGCAGCCC TCAATGGAGC TCCTGGAACC CCTTCAGATG ACGAAGAGCT GCCTCATCCT 
CTACCCATCT CATCAGCAGG CCCTTCCCCA ACAGCCTCCC TGGGGTCCAA TAGAAATGGG 
GAGGGGAGCA GCATTCCCTC CCCCGACACA GAAATCTATG GTGGCACCCT GGACGACGAG 
GCCCCTTCTT CTCCAGACCT GCTCCAGGGG TCTTTCAGCG AGCAGCTTGA TGCCATCGAG 
GCTCCTAAGG GCCCAGGTGA CAGACCTCTA GGAGCAGCTT CTCCAAAACT CCGCTCCAGC 
TTTCCCACAC ATACGCGACT CAGTGCCATG CTGCACATCG ACTCAGATGA AGATGAGGAC 
AGATCTGCTG CTACAGAGGC CACGTTGTCA ACATTTAACG GCATTCCAAG GTCTTATCAA 
ACACCCCCAA AAGCAGCAGC AAGAGGAGCT GTGTTTGATG GAGGCATAGA GGAACCTTCA 
GAAGAGGCTG GGCTGGAGTC TGAAGTAGAA ACTCTGAATG CAGGAGAAGA GGTGCCTCCT 
GAGCCAGAGG TCGTGCCCAG TTCAGTCGCT CCCGAGGTGG AAACCGCCAG TCTGATGGAG 
AGGCAGGAGG AACAGGGCCG GGAGGTGGAG GTAGAGGAGG AGGAGGAAGA GGGCCTGGCG 
CGCAGTGAAG AGTTGGCAAC CGAAGTTGAT ATCTCCTCAA TGGCATCTGA CTGCTGCCCG 
GGGCCTGCAT CAGCATCTCA GGAGGCGGAG CAGGGGGCAG AGGCAGCCAT TGACCCAGGG 
GGAGAAGATC TCTCAGATGA TCCACCTACC ACTTACGAGG TTGCAGAGAC TGCTGAAGAA 
ACACATCTAA CTAATGAGCC GGAGAGAGAG GCTCCAAAAC CAGAGCCCCC CACCAACGAG 
GAAGAGGAAT GTGAAGATGT GACTGAGGAT ACCATGTCCA GAAGATGGAG CCTGGAGGCC 
ACAGCAGGAG TGTCACAGGA GGATGAAGAT GAGTTGATAC CATCCGGAGA TGGGGAATCT 
GTGGACTCTG AGACTTTTGT TACGGAGACA GAGTCAGAAT TAGGCGTTCC TCAGGTAAAC 
GGAGATCAGC GTGTTAATTC TCTGCCATCT GTAAGACAAG ACATCCACCG ATACCAGCGT 
GTGGACGAGC CCTTACCTCC CAACTGGGAG GCTCGTATTG ACAGTCATGG TCGTATCTTC 
TTTGTGGATC ACGTGAATCG CACCACCACA TGGCAGAGAC CCACTGGCCC TCCGGCTCCA 
CAGGGTCTGA CCCGCTCCAG CTCCATTCTG CAGATGGAAC AACTCAACCG CAGGTATCAG 
AGTATTCGCA GGACAATGAC CAGTGAAAGG TCTGAGGAAC CCAGAGTTGA AGATCCTCCA 
GCTGATGAAA CAGACGTGCT GGCTCAGTCC ATCAGTGAAT ACCGGAGGGA TGGCATTATT 
GGTCAGTCCA GCTCACGCTC TCGTCTGGCT CTTCTGCTTC AGTCTCCTGG TGCAAAGTTC 
CTGTCCAGTC CAGACTTCTT CTCTGTTCTG CATTCTAATC CTAGTGCCTA TCGGATGTTC 
ACAAGTAACA CGTGTCTGAA GCACATGATC AGTAAAGTTC GACGGGATGC CCAACACTTT 
GAGCGGTACC AGCACAACCG AGACCTGGTC AACTTCCTCA ATCTGTTCTC CAACAAGCAG 
CTGGAGCTTC CCCGGGGCTG GGAGATGAAA CATGACCATA CTGGGAAGGC CTTCTTTGTA 
GACCACAACT GTCGTGCCAC AACATTTATT GACCCGCGGC TGCCCTTGCA GAACTCGCGG 
TCCAGCGGAT TGTTGGCTCA CAGACAGCAC CTGTCCCGCC AGCGCAGCCA CAGTGCAGGA 
GAGGTTGGAG ATGACTCTCG TTCCCCGAGT CCTCCAGTCC AGGGCCGGTC ATCTAGAAAC 
ACCTATAATG ATAAGATTGT TGCCTTTTTG CGACAACCCA ACATTTTTGA GATCCTGCAA 
GAGAGACAGC CAGAACTTGT GAGAAACCAT TCCCTTAGGG AGAAGGTTCA GTTTATCCGC 
AATGAGGGGG CAACAGGGTT AACTCGTCTG TCCAGTGATG CAGACCTGGT TATGTTGCTC 
AGCCTGTTTG AAGAGGAAGT GATGTCGTAT GTTCCGCCCA ATGCCTTACT GCTCTCCAGC 
TACTGTCATG CGTCCCCACA GAGCTCCCCT GGAACCCCAC GGGCTAATGC GCGAGCCCCT 
GCTCCCTACA AGCGGGATTT TGAGGCCAAA TTAAGAAACT TCTACCGCAA AATGGAGACC 
AAGGGCTACG GACAGGGACC AGGAAAAGTG AAGTTAATAA TAAGAAGAGA TCACCTGCTT 
GAGGACGCTT TCAATCAGAT CATGTGTTAT TCCCGCAAAG ACCTTCAAAG GAGCAGGCTT 
TACGTCAGCT TTGTTGGAGA AGAAGGATTG GACTATAGTG GTCCATCCAG GGAGTTCTTC 
TTCCTGGTTT CCCGTGAGCT TTTTAACCCA TACTATGGAC TCTTTGAATA CTCAGCTAAT 
GACACTTACA CTGTGCAGAT CAGCCCTATG TCAGCCTTTG TGGACAACCA TCATGAGTGG 
TTCCGGTTCA GTGGCCGCAT TCTGGGTCTT GCTCTAATTC ATCAGTACCT GCTGGATGCC 
TTCTTCACCC GACCATTTTA CAAAGGCCTT CTCCGCATTC CATGTGATCT GAGTGATCTA 
GAGTTTCTGG ATGAAGAGTT TCATCAGAGT TTGCAGTGGA TGAAGGATAA TGACATCGAG 
GACATGCTGG ACCTCACCTT TACAGTCAAT GAGGAGGTGT TTGGACAGAT AACAGAACGT 
GAGCTAAAGC CGGGTGGATC TGGCATCGCT GTGTCAGACA AGAACAAGAA AGAGTACATT 
GAGCGCATGG TGAAGTGGCG GATTGAGAGA GGTGTGGCTC AGCAGACAGA GAGTCTTGTG 
CGAGGATTTT ATGAGGTGGT GGATGTGCGG CTCGTCTCAG TGTTTGATGC CCGAGAGCTG 
GAGCTGGTCA TTGCTGGAAC AGCAGAGATA GACCTGTCCG ACTGGAGAAA CAACACAGAA 
TACAGGGGAG GTTATCATGA CAACCACATA GTGATTCGAT GGTTCTGGGC TGCTGTGGAG 
AGATTCAACA ATGAACAGAG ACTACGACTC CTTCAGTTTG TAACAGGAAC TTCCAGCATT 
CCCTATGAGG GCTTTGCCTC TCTGCGGGGC AGTAATGGTC CACGCCGATT CTGTGTGGAG 
AAATGGGGCA AAGTGACCTC CCTCCCACGG GCTCACACCT GCTTTAACCG ACTGGATCTC 
CCTCCCTACC CCTCGTTCTC CATGCTCTAT GAGAAGATGG TCACGGCTGT GGAGGAAACC 
AGCACCTTTG GTCTGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302977.1
CDS	926..5326
Translation	MAPQGRDHLATRESAGRRRSPNVRHTLSPENLRSLAERGGAEQAGVGLLRANSDTDLVSSQGRSSLTASTLEFTLCRGQNLVISWDIKEEVDATDWIGLYHIDETCPSNVWDCKNRGVNGTQCGQIVWRLEVGPYFLESETRICFKYYHGVSGALRATTPCITVKNPRASVDGQGEGQQASDSSRKLVSFTLSDIRAVGLKKGMFFNPDPYLKMSIHPGKRSCFPTFSHHGQERRSGITTNTTNPVWHGEKHTFVALMTDVLVIEVKDKFAKSRPIIKRFLGQLNIPVQLLLERHSTGNQSLSFSLCRRLPTDHVSGQMHFKVDVTSMGPDDVSPETILGAAALNGAPGTPSDDEELPHPLPISSAGPSPTASLGSNRNGEGSSIPSPDTEIYGGTLDDEAPSSPDLLQGSFSEQLDAIEAPKGPGDRPLGAASPKLRSSFPTHTRLSAMLHIDSDEDEDRSAATEATLSTFNGIPRSYQTPPKAAARGAVFDGGIEEPSEEAGLESEVETLNAGEEVPPEPEVVPSSVAPEVETASLMERQEEQGREVEVEEEEEEGLARSEELATEVDISSMASDCCPGPASASQEAEQGAEAAIDPGGEDLSDDPPTTYEVAETAEETHLTNEPEREAPKPEPPTNEEEECEDVTEDTMSRRWSLEATAGVSQEDEDELIPSGDGESVDSETFVTETESELGVPQVNGDQRVNSLPSVRQDIHRYQRVDEPLPPNWEARIDSHGRIFFVDHVNRTTTWQRPTGPPAPQGLTRSSSILQMEQLNRRYQSIRRTMTSERSEEPRVEDPPADETDVLAQSISEYRRDGIIGQSSSRSRLALLLQSPGAKFLSSPDFFSVLHSNPSAYRMFTSNTCLKHMISKVRRDAQHFERYQHNRDLVNFLNLFSNKQLELPRGWEMKHDHTGKAFFVDHNCRATTFIDPRLPLQNSRSSGLLAHRQHLSRQRSHSAGEVGDDSRSPSPPVQGRSSRNTYNDKIVAFLRQPNIFEILQERQPELVRNHSLREKVQFIRNEGATGLTRLSSDADLVMLLSLFEEEVMSYVPPNALLLSSYCHASPQSSPGTPRANARAPAPYKRDFEAKLRNFYRKMETKGYGQGPGKVKLIIRRDHLLEDAFNQIMCYSRKDLQRSRLYVSFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLRIPCDLSDLEFLDEEFHQSLQWMKDNDIEDMLDLTFTVNEEVFGQITERELKPGGSGIAVSDKNKKEYIERMVKWRIERGVAQQTESLVRGFYEVVDVRLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKVTSLPRAHTCFNRLDLPPYPSFSMLYEKMVTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009304702.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2a (hecw2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304702.2

ORF Insert Sequence:

ATGGCGCCTC AGGGGCGGGA TCACCTGGCC ACACGGGAGT CTGCAGGCCG AAGACGCAGC 
CCGAATGTGC GGCACACGCT CAGCCCGGAA AACCTGCGAA GCCTGGCGGA GCGCGGTGGA 
GCTGAACAAG CGGGAGTAGG ACTGCTCAGA GCCAATAGCG ACACAGACCT GGTCAGTTCT 
CAAGGGCGAT CCTCACTCAC AGCCTCCACT CTAGAGTTTA CACTGTGCCG CGGACAGAAC 
CTCGTCATCT CATGGGACAT CAAGGAGGAG GTGGACGCCA CTGACTGGAT CGGCCTGTAC 
CACATAGATG AAACCTGTCC ATCAAATGTG TGGGACTGTA AGAACCGTGG GGTAAACGGC 
ACACAGTGTG GTCAGATCGT CTGGAGATTA GAGGTCGGGC CATACTTTCT TGAATCTGAG 
ACAAGGATCT GCTTTAAATA CTACCATGGA GTAAGTGGAG CATTGAGAGC CACGACTCCC 
TGCATCACAG TCAAGAACCC CAGAGCCTCG GTTGATGGCC AAGGAGAAGG ACAACAAGCT 
TCAGACAGTA GTCGAAAACT GGTTAGCTTT ACGCTATCAG ATATCCGAGC TGTTGGCCTG 
AAGAAGGGGA TGTTTTTTAA CCCAGACCCT TATCTGAAGA TGAGCATTCA TCCAGGCAAA 
AGAAGCTGCT TTCCGACCTT TTCCCATCAC GGCCAAGAGA GGCGATCCGG CATTACAACT 
AACACCACTA ATCCTGTTTG GCATGGAGAG AAACATACTT TTGTGGCTCT TATGACAGAT 
GTATTAGTGA TTGAGGTTAA AGACAAGTTT GCTAAAAGTC GACCCATTAT TAAACGCTTC 
TTGGGTCAGC TGAATATTCC TGTGCAGCTG CTGCTGGAGA GACATTCGAC AGGGAACCAG 
TCACTCAGCT TCTCCCTATG TCGTCGTTTG CCCACTGATC ATGTGAGTGG TCAAATGCAC 
TTTAAAGTGG ATGTCACGTC TATGGGCCCA GATGATGTGT CTCCCGAGAC AATTCTGGGG 
GCAGCAGCCC TCAATGGAGC TCCTGGAACC CCTTCAGATG ACGAAGAGCT GCCTCATCCT 
CTACCCATCT CATCAGCAGG CCCTTCCCCA ACAGCCTCCC TGGGGTCCAA TAGAAATGGG 
GAGGGGAGCA GCATTCCCTC CCCCGACACA GAAATCTATG GTGGCACCCT GGACGACGAG 
GCCCCTTCTT CTCCAGACCT GCTCCAGGGG TCTTTCAGCG AGCAGCTTGA TGCCATCGAG 
GCTCCTAAGG GCCCAGGTGA CAGACCTCTA GGAGCAGCTT CTCCAAAACT CCGCTCCAGC 
TTTCCCACAC ATACGCGACT CAGTGCCATG CTGCACATCG ACTCAGATGA AGATGAGGAC 
AGATCTGCTG CTACAGAGGC CACGTTGTCA ACATTTAACG GCATTCCAAG GTCTTATCAA 
ACACCCCCAA AAGCAGCAGC AAGAGGAGCT GTGTTTGATG GAGGCATAGA GGAACCTTCA 
GAAGAGGCTG GGCTGGAGTC TGAAGTAGAA ACTCTGAATG CAGGAGAAGA GGTGCCTCCT 
GAGCCAGAGG TCGTGCCCAG TTCAGTCGCT CCCGAGGTGG AAACCGCCAG TCTGATGGAG 
AGGCAGGAGG AACAGGGCCG GGAGGTGGAG GTAGAGGAGG AGGAGGAAGA GGGCCTGGCG 
CGCAGTGAAG AGTTGGCAAC CGAAGTTGAT ATCTCCTCAA TGGCATCTGA CTGCTGCCCG 
GGGCCTGCAT CAGCATCTCA GGAGGCGGAG CAGGGGGCAG AGGCAGCCAT TGACCCAGGG 
GGAGAAGATC TCTCAGATGA TCCACCTACC ACTTACGAGG TTGCAGAGAC TGCTGAAGAA 
ACACATCTAA CTAATGAGCC GGAGAGAGAG GCTCCAAAAC CAGAGCCCCC CACCAACGAG 
GAAGAGGAAT GTGAAGATGT GACTGAGGAT ACCATGTCCA GAAGATGGAG CCTGGAGGCC 
ACAGCAGGAG TGTCACAGGA GGATGAAGAT GAGTTGATAC CATCCGGAGA TGGGGAATCT 
GTGGACTCTG AGACTTTTGT TACGGAGACA GAGTCAGAAT TAGGCGTTCC TCAGGTAAAC 
GGAGATCAGC GTGTTAATTC TCTGCCATCT GTAAGACAAG ACATCCACCG ATACCAGCGT 
GTGGACGAGC CCTTACCTCC CAACTGGGAG GCTCGTATTG ACAGTCATGG TCGTATCTTC 
TTTGTGGATC ACGTGAATCG CACCACCACA TGGCAGAGAC CCACTGGCCC TCCGGCTCCA 
CAGGGTCTGA CCCGCTCCAG CTCCATTCTG CAGATGGAAC AACTCAACCG CAGGTATCAG 
AGTATTCGCA GGACAATGAC CAGTGAAAGG TCTGAGGAAC CCAGAGTTGA AGATCCTCCA 
GCTGATGAAA CAGACGTGCT GGCTCAGTCC ATCAGTGAAT ACCGGAGGGA TGGCATTATT 
GGTCAGTCCA GCTCACGCTC TCGTCTGGCT CTTCTGCTTC AGTCTCCTGG TGCAAAGTTC 
CTGTCCAGTC CAGACTTCTT CTCTGTTCTG CATTCTAATC CTAGTGCCTA TCGGATGTTC 
ACAAGTAACA CGTGTCTGAA GCACATGATC AGTAAAGTTC GACGGGATGC CCAACACTTT 
GAGCGGTACC AGCACAACCG AGACCTGGTC AACTTCCTCA ATCTGTTCTC CAACAAGCAG 
CTGGAGCTTC CCCGGGGCTG GGAGATGAAA CATGACCATA CTGGGAAGGC CTTCTTTGTA 
GACCACAACT GTCGTGCCAC AACATTTATT GACCCGCGGC TGCCCTTGCA GAACTCGCGG 
TCCAGCGGAT TGTTGGCTCA CAGACAGCAC CTGTCCCGCC AGCGCAGCCA CAGTGCAGGA 
GAGGTTGGAG ATGACTCTCG TTCCCCGAGT CCTCCAGTCC AGGGCCGGTC ATCTAGAAAC 
ACCTATAATG ATAAGATTGT TGCCTTTTTG CGACAACCCA ACATTTTTGA GATCCTGCAA 
GAGAGACAGC CAGAACTTGT GAGAAACCAT TCCCTTAGGG AGAAGGTTCA GTTTATCCGC 
AATGAGGGGG CAACAGGGTT AACTCGTCTG TCCAGTGATG CAGACCTGGT TATGTTGCTC 
AGCCTGTTTG AAGAGGAAGT GATGTCGTAT GTTCCGCCCA ATGCCTTACT GCTCTCCAGC 
TACTGTCATG CGTCCCCACA GAGCTCCCCT GGAACCCCAC GGGCTAATGC GCGAGCCCCT 
GCTCCCTACA AGCGGGATTT TGAGGCCAAA TTAAGAAACT TCTACCGCAA AATGGAGACC 
AAGGGCTACG GACAGGGACC AGGAAAAGTG AAGTTAATAA TAAGAAGAGA TCACCTGCTT 
GAGGACGCTT TCAATCAGAT CATGTGTTAT TCCCGCAAAG ACCTTCAAAG GAGCAGGCTT 
TACGTCAGCT TTGTTGGAGA AGAAGGATTG GACTATAGTG GTCCATCCAG GGAGTTCTTC 
TTCCTGGTTT CCCGTGAGCT TTTTAACCCA TACTATGGAC TCTTTGAATA CTCAGCTAAT 
GACACTTACA CTGTGCAGAT CAGCCCTATG TCAGCCTTTG TGGACAACCA TCATGAGTGG 
TTCCGGTTCA GTGGCCGCAT TCTGGGTCTT GCTCTAATTC ATCAGTACCT GCTGGATGCC 
TTCTTCACCC GACCATTTTA CAAAGGCCTT CTCCGCATTC CATGTGATCT GAGTGATCTA 
GAGTTTCTGG ATGAAGAGTT TCATCAGAGT TTGCAGTGGA TGAAGGATAA TGACATCGAG 
GACATGCTGG ACCTCACCTT TACAGTCAAT GAGGAGGTGT TTGGACAGAT AACAGAACGT 
GAGCTAAAGC CGGGTGGATC TGGCATCGCT GTGTCAGACA AGAACAAGAA AGAGTACATT 
GAGCGCATGG TGAAGTGGCG GATTGAGAGA GGTGTGGCTC AGCAGACAGA GAGTCTTGTG 
CGAGGATTTT ATGAGGTGGT GGATGTGCGG CTCGTCTCAG TGTTTGATGC CCGAGAGCTG 
GAGCTGGTCA TTGCTGGAAC AGCAGAGATA GACCTGTCCG ACTGGAGAAA CAACACAGAA 
TACAGGGGAG GTTATCATGA CAACCACATA GTGATTCGAT GGTTCTGGGC TGCTGTGGAG 
AGATTCAACA ATGAACAGAG ACTACGACTC CTTCAGTTTG TAACAGGAAC TTCCAGCATT 
CCCTATGAGG GCTTTGCCTC TCTGCGGGGC AGTAATGGTC CACGCCGATT CTGTGTGGAG 
AAATGGGGCA AAGTGACCTC CCTCCCACGG GCTCACACCT GCTTTAACCG ACTGGATCTC 
CCTCCCTACC CCTCGTTCTC CATGCTCTAT GAGAAGATGG TCACGGCTGT GGAGGAAACC 
AGCACCTTTG GTCTGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HECW2A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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