LOC109484912 cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109484912
Entrez Gene ID	109484912
Full Name	thrombospondin-2-like
Gene Type	protein-coding
Organism	Branchiostoma belcheri(Belcher's lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019788263.1	XP_019643822.1	thrombospondin-2-like

Pathways from BioSystems
Gene Ontology

The following LOC109484912 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109484912 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr195075	XM_019788263.1 Latest version!	Branchiostoma belcheri thrombospondin-2-like (LOC109484912), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109484912 ( XM_019788263.1 ) cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet) -> XP_019643822.1 Branchiostoma belcheri thrombospondin-2-like (LOC109484912), mRNA.

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CloneID	OBr195075
Clone ID Related Accession (Same CDS sequence)	XM_019788263.1
Accession Version	XM_019788263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Branchiostoma belcheri(Belcher's lancelet)
Product	thrombospondin-2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017804365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Branchiostoma belcheri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGAG AGAGAGTTGT GAGATTAGCG CTGTGCGTGC TGTACGTGTT AGCTGTAACC 
GAGCTGGTGA TCGCACAGGA GAAAGTGCGG GTAAAAAGAG CGATGTTTGA CCTGTTTGAA 
TCGACGAGTC TTGGGGCGAG CACACAGGGA GTGAAGAAAG TGCGGGGGCT CGATCAGTAT 
TCACCGGCCT ACAAGTTCCA CAACCGGCCC GACGTCGACT TGCCGGCCAG CCCCGAGGCC 
CAGGCCAGCA TCGTCGACGC GATGATGTCG AATCACGGCT TTGTGTTCGT CGCGGCCCTT 
CGGCAGGACC GGCGGAACCG TGGCACGCTC GTGTCGGTCG ACTCGCCCGA CGGCAGGACC 
AGATACTTCG GCATCCAGAG TGACGGCCAG ACGCCCTCGG TGGACGTGGT GTACATGCAC 
GAGGGCAAGG AAGTGGCGGC CTCGTTCGAC GGCGTCAACT TCGCCACCCG GGATTGGCGC 
AACCTGACCT TGGTGGTGCA GGGGGACCTG ATCGCGCTCT TCGACGGATG TACCAAGGTG 
GGCGAGCAGG GCCTGATGGG GAACATCTTC CAGACCGTCC TCCAGCCCGG ACAGAGCGAG 
CTCCGCCTGG GGAAGGGTCT ACCGGCCCGG GAGGCCTTCG AAGACTACAG GGGTGTGATG 
CAGAGTGTGA AGTTCATCTT TGGAATCCCG GTGTCCAACA TTCTCCACCT GCAGGGCTGC 
GCACCGGACA CAGGTTCAGA ATACAACACA GGTAACATCA CCCAGACCGC AGCTGCCAAG 
AGCAGCGTCC TGACCAGGAA GTTCAAGCTC ATCCCCCAGC CCACCGACGA GTGCGAAGTC 
TCCTGCGAAG ACCTGGACGA CTTGAAGAAA CAGATCAAGG ATCTTAGAAT TCACCTCAAC 
AACGTGGAGG ATGGACTACA GAAGATGTGG GAAGAGAACA ACATCTTGAG GAAGCAGATC 
GGCGCTCCTC CACATGGAGG CTGCTGGGCG GAAGGCCGTT TCTATGACAA CCATGCGGAC 
TGGGTGGAGA ACCGCTGTAC CAAGTGTCAC TGTCAGGATT CCAGAACTAC ATGTCAGAAG 
ATCGAGTGCC CGGCAGTCCA ATGTCTCAAC CCAGTTTTCG ACAGCGAGTC TGACTGTTGC 
CCCTACTGTC CCCTCAGTGG GGACGACGCG GAGGTCGGAT GGAGTCAGTG GTCCGACTGG 
ACGCCGTGCA GCGCCACTTG CGGACCCGGC ACACAGCAGC GAGGCCGATC GTGCGACCGT 
GTCAACTACC CCTGCGACGG TCCCAGCGTA GAGACCAAAG TGTGCCTACA GGACGACTGC 
GACAACAGAA TCCCCCAGAA TGGAGGCTGG AGTTTGTGGA CGCCGTGGAC ATGCTCTGTG 
ACCTGTGGCG AGGGCATCAC CACCCGCATC CGGACCTGCA ATGCACCCAC TCCGCAGCTA 
GGTGGCGAGA ACTGTCTTGG AGACGGACGA GAGAACAACA TTTGCGAGAG GGAGGCATGC 
CCTGTTCACG GCCAGTGGGG TCAGTGGTCG CTCTGGGGCC CGTGTTCGCG TACCTGTGAC 
GGAGGCACCA GGGTCCGACA GCGCCTGTGT GACAGTCCCG CGCCGGCGTA CGGAGGAGAC 
AAGTGCCAGG GCCAGCCCGC CGACGTGGAC CACTGCAACA CCGAGCCTTG CCCCATTGAT 
GGATGCTTGT CCAACCCCTG CTTCGCCGGA GTGGAATGTT CCAGTACGAA GGACGGGAAG 
TACACGTGCG GTCCCTGTCC CGCCGGCTTC GAGGGTGACG GAGAGTCTTG CACTGACATT 
GATGAGTGTG CTCTGGTTCC TGACACCTGC TATGTCCACA ACGGAGTGTC CCTGTGTAAG 
AACCTGGGCC CGGGTTACCG GTGCCAGAGG TGTCCTCCAG GCTACACTGG CAACCAGCCC 
GGCGGAGTTG GTATCTTCAT GGCAGAAAGC AACAAACAGA CATGCCTGCC CGCCAACCCC 
TGCAGGGACG GAACTGACCA GTGCCACTTC AACGCTGAGT GCAAGTTCCT CGGTCCGTTC 
ATTGACCCAC TGTACGAGTG CCAGTGTAAG GTTGGATATG CTGGGAATGG CATCCACTGT 
GGCGAGGATA TTGACTTGGA TGGTTATCCT GACGAAGACC TGCCCTGTCT GATCGGCAAC 
ACAACCCACT GCAAAAAGGA CAACTGCCCA TTCCTACCTA ACAGTGGACA GGAGGACTTT 
GACAAGGATG GCCAGGGTGA TGCCTGCGAT AGAGATGATG ATAACGATGG CATTCCTGAC 
AATCGGGACA ACTGCCCGAT GGCGTTCAAT GCCAGACAGG ACGACTACGA CCGCGATGGA 
TGGGGCGACC TGTGTGACAA CTGCCCGTAC GACCAGAACT TCTACCAGAA GGACACAGAT 
GGCAACGGAG AGGGAGACGC CTGCTCTGTA GACATAGATG GTGATGGACT GCGGAATGAA 
GTTGACAACT GCCCGCTGCA TTTCAACCTC AGACAGGAGG ATATTGACGG TGATGGAGTC 
GGGGACGCGT GCGACAACTG CATAGTCATC GCCAACCCCA AACAAGAGGA CTCCGACTCG 
GATCTGTTTG GCGATGCTTG CGACAACGAC ATTGACGTGG ACGATGACGG TCTGGAGAAT 
ATCCGGGACA ACTGCCCGAG TATCCCCAAC AGTAATCAGG TCAACCACGA CGGTGACGAA 
TTTGGCGATG CCTGTGATGA TGATGATGAC AACGACGGCA TTTTGGATAA GGATGACAAC 
TGCAGACTCC GCGCAAACCC AGACCAAATA GACAGCAACG GTGACGGACG TGGAGACGTC 
TGCCAGGAAG ACTTTGACGG TGACGGTGTC AAGGACAAGG ATGATGTGTG CCCAGAGAAC 
GCCCAGATCT CCAGGGTAGA CTTCCGTGAC TTCCAGATGG TCGCACTGGA TCCAAAGGGA 
ACATCGCAGA TCGACCCCAA CTGGGTCATC AGGAACCAAG GGAAGGAGCT GGTGCAGACA 
GTCAACAGTG ATCCGGGCAT GGCCATAGGA GTTGACAGGT TCAACGAAGT GGACTTTGAC 
GGCACCTTCT ACGTGAACAC ACAAAAGGAC GACGACTACG CGGGCTTCGT GTTCGGCTAC 
CAGTCCAGCC ATCGCTTCTA CGTGGTGATG TGGAAACAGG TCCACCAGAC GTACTGGCTC 
TCCAAACCTT CCATGGCCCA CGGCTCAGCA GGAGTCCAAG TCAAGGTGGT AAACTCGGAG 
ACAGGACCAG GAGAGGCCCT GAGGAATGCC CTGTGGCACA CTGGAGACAC TCCCGGACAG 
GTGAGACTGC TCTGGCACGA CAAGAACACC ATTGGATGGA AAGACATGAC TGCCTACAGA 
TGGAAGCTGA AGCATAAACC AGACTCTGGA CTAATAAGGG TGACGTTCTA TGAAGGTAGT 
AAAGTTATGG CCGACTCTGG GGATCTATTC GACACAACAT TAAAGGGAGG TCGCCTAGGA 
CTCTTCGTCT TTTCACAAGA GATGGTCTTC TTCTCCGATC TGAAGTACAA ATGTGGTCAG 
CAAGAGGCCT TGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019643822.1
CDS	481..4038
Translation	MWRERVVRLALCVLYVLAVTELVIAQEKVRVKRAMFDLFESTSLGASTQGVKKVRGLDQYSPAYKFHNRPDVDLPASPEAQASIVDAMMSNHGFVFVAALRQDRRNRGTLVSVDSPDGRTRYFGIQSDGQTPSVDVVYMHEGKEVAASFDGVNFATRDWRNLTLVVQGDLIALFDGCTKVGEQGLMGNIFQTVLQPGQSELRLGKGLPAREAFEDYRGVMQSVKFIFGIPVSNILHLQGCAPDTGSEYNTGNITQTAAAKSSVLTRKFKLIPQPTDECEVSCEDLDDLKKQIKDLRIHLNNVEDGLQKMWEENNILRKQIGAPPHGGCWAEGRFYDNHADWVENRCTKCHCQDSRTTCQKIECPAVQCLNPVFDSESDCCPYCPLSGDDAEVGWSQWSDWTPCSATCGPGTQQRGRSCDRVNYPCDGPSVETKVCLQDDCDNRIPQNGGWSLWTPWTCSVTCGEGITTRIRTCNAPTPQLGGENCLGDGRENNICEREACPVHGQWGQWSLWGPCSRTCDGGTRVRQRLCDSPAPAYGGDKCQGQPADVDHCNTEPCPIDGCLSNPCFAGVECSSTKDGKYTCGPCPAGFEGDGESCTDIDECALVPDTCYVHNGVSLCKNLGPGYRCQRCPPGYTGNQPGGVGIFMAESNKQTCLPANPCRDGTDQCHFNAECKFLGPFIDPLYECQCKVGYAGNGIHCGEDIDLDGYPDEDLPCLIGNTTHCKKDNCPFLPNSGQEDFDKDGQGDACDRDDDNDGIPDNRDNCPMAFNARQDDYDRDGWGDLCDNCPYDQNFYQKDTDGNGEGDACSVDIDGDGLRNEVDNCPLHFNLRQEDIDGDGVGDACDNCIVIANPKQEDSDSDLFGDACDNDIDVDDDGLENIRDNCPSIPNSNQVNHDGDEFGDACDDDDDNDGILDKDDNCRLRANPDQIDSNGDGRGDVCQEDFDGDGVKDKDDVCPENAQISRVDFRDFQMVALDPKGTSQIDPNWVIRNQGKELVQTVNSDPGMAIGVDRFNEVDFDGTFYVNTQKDDDYAGFVFGYQSSHRFYVVMWKQVHQTYWLSKPSMAHGSAGVQVKVVNSETGPGEALRNALWHTGDTPGQVRLLWHDKNTIGWKDMTAYRWKLKHKPDSGLIRVTFYEGSKVMADSGDLFDTTLKGGRLGLFVFSQEMVFFSDLKYKCGQQEALN

Target ORF information:

RefSeq Version	XM_019788263.1
Organism	Branchiostoma belcheri(Belcher's lancelet)
Definition	Branchiostoma belcheri thrombospondin-2-like (LOC109484912), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019788263.1

ORF Insert Sequence:

ATGTGGAGAG AGAGAGTTGT GAGATTAGCG CTGTGCGTGC TGTACGTGTT AGCTGTAACC 
GAGCTGGTGA TCGCACAGGA GAAAGTGCGG GTAAAAAGAG CGATGTTTGA CCTGTTTGAA 
TCGACGAGTC TTGGGGCGAG CACACAGGGA GTGAAGAAAG TGCGGGGGCT CGATCAGTAT 
TCACCGGCCT ACAAGTTCCA CAACCGGCCC GACGTCGACT TGCCGGCCAG CCCCGAGGCC 
CAGGCCAGCA TCGTCGACGC GATGATGTCG AATCACGGCT TTGTGTTCGT CGCGGCCCTT 
CGGCAGGACC GGCGGAACCG TGGCACGCTC GTGTCGGTCG ACTCGCCCGA CGGCAGGACC 
AGATACTTCG GCATCCAGAG TGACGGCCAG ACGCCCTCGG TGGACGTGGT GTACATGCAC 
GAGGGCAAGG AAGTGGCGGC CTCGTTCGAC GGCGTCAACT TCGCCACCCG GGATTGGCGC 
AACCTGACCT TGGTGGTGCA GGGGGACCTG ATCGCGCTCT TCGACGGATG TACCAAGGTG 
GGCGAGCAGG GCCTGATGGG GAACATCTTC CAGACCGTCC TCCAGCCCGG ACAGAGCGAG 
CTCCGCCTGG GGAAGGGTCT ACCGGCCCGG GAGGCCTTCG AAGACTACAG GGGTGTGATG 
CAGAGTGTGA AGTTCATCTT TGGAATCCCG GTGTCCAACA TTCTCCACCT GCAGGGCTGC 
GCACCGGACA CAGGTTCAGA ATACAACACA GGTAACATCA CCCAGACCGC AGCTGCCAAG 
AGCAGCGTCC TGACCAGGAA GTTCAAGCTC ATCCCCCAGC CCACCGACGA GTGCGAAGTC 
TCCTGCGAAG ACCTGGACGA CTTGAAGAAA CAGATCAAGG ATCTTAGAAT TCACCTCAAC 
AACGTGGAGG ATGGACTACA GAAGATGTGG GAAGAGAACA ACATCTTGAG GAAGCAGATC 
GGCGCTCCTC CACATGGAGG CTGCTGGGCG GAAGGCCGTT TCTATGACAA CCATGCGGAC 
TGGGTGGAGA ACCGCTGTAC CAAGTGTCAC TGTCAGGATT CCAGAACTAC ATGTCAGAAG 
ATCGAGTGCC CGGCAGTCCA ATGTCTCAAC CCAGTTTTCG ACAGCGAGTC TGACTGTTGC 
CCCTACTGTC CCCTCAGTGG GGACGACGCG GAGGTCGGAT GGAGTCAGTG GTCCGACTGG 
ACGCCGTGCA GCGCCACTTG CGGACCCGGC ACACAGCAGC GAGGCCGATC GTGCGACCGT 
GTCAACTACC CCTGCGACGG TCCCAGCGTA GAGACCAAAG TGTGCCTACA GGACGACTGC 
GACAACAGAA TCCCCCAGAA TGGAGGCTGG AGTTTGTGGA CGCCGTGGAC ATGCTCTGTG 
ACCTGTGGCG AGGGCATCAC CACCCGCATC CGGACCTGCA ATGCACCCAC TCCGCAGCTA 
GGTGGCGAGA ACTGTCTTGG AGACGGACGA GAGAACAACA TTTGCGAGAG GGAGGCATGC 
CCTGTTCACG GCCAGTGGGG TCAGTGGTCG CTCTGGGGCC CGTGTTCGCG TACCTGTGAC 
GGAGGCACCA GGGTCCGACA GCGCCTGTGT GACAGTCCCG CGCCGGCGTA CGGAGGAGAC 
AAGTGCCAGG GCCAGCCCGC CGACGTGGAC CACTGCAACA CCGAGCCTTG CCCCATTGAT 
GGATGCTTGT CCAACCCCTG CTTCGCCGGA GTGGAATGTT CCAGTACGAA GGACGGGAAG 
TACACGTGCG GTCCCTGTCC CGCCGGCTTC GAGGGTGACG GAGAGTCTTG CACTGACATT 
GATGAGTGTG CTCTGGTTCC TGACACCTGC TATGTCCACA ACGGAGTGTC CCTGTGTAAG 
AACCTGGGCC CGGGTTACCG GTGCCAGAGG TGTCCTCCAG GCTACACTGG CAACCAGCCC 
GGCGGAGTTG GTATCTTCAT GGCAGAAAGC AACAAACAGA CATGCCTGCC CGCCAACCCC 
TGCAGGGACG GAACTGACCA GTGCCACTTC AACGCTGAGT GCAAGTTCCT CGGTCCGTTC 
ATTGACCCAC TGTACGAGTG CCAGTGTAAG GTTGGATATG CTGGGAATGG CATCCACTGT 
GGCGAGGATA TTGACTTGGA TGGTTATCCT GACGAAGACC TGCCCTGTCT GATCGGCAAC 
ACAACCCACT GCAAAAAGGA CAACTGCCCA TTCCTACCTA ACAGTGGACA GGAGGACTTT 
GACAAGGATG GCCAGGGTGA TGCCTGCGAT AGAGATGATG ATAACGATGG CATTCCTGAC 
AATCGGGACA ACTGCCCGAT GGCGTTCAAT GCCAGACAGG ACGACTACGA CCGCGATGGA 
TGGGGCGACC TGTGTGACAA CTGCCCGTAC GACCAGAACT TCTACCAGAA GGACACAGAT 
GGCAACGGAG AGGGAGACGC CTGCTCTGTA GACATAGATG GTGATGGACT GCGGAATGAA 
GTTGACAACT GCCCGCTGCA TTTCAACCTC AGACAGGAGG ATATTGACGG TGATGGAGTC 
GGGGACGCGT GCGACAACTG CATAGTCATC GCCAACCCCA AACAAGAGGA CTCCGACTCG 
GATCTGTTTG GCGATGCTTG CGACAACGAC ATTGACGTGG ACGATGACGG TCTGGAGAAT 
ATCCGGGACA ACTGCCCGAG TATCCCCAAC AGTAATCAGG TCAACCACGA CGGTGACGAA 
TTTGGCGATG CCTGTGATGA TGATGATGAC AACGACGGCA TTTTGGATAA GGATGACAAC 
TGCAGACTCC GCGCAAACCC AGACCAAATA GACAGCAACG GTGACGGACG TGGAGACGTC 
TGCCAGGAAG ACTTTGACGG TGACGGTGTC AAGGACAAGG ATGATGTGTG CCCAGAGAAC 
GCCCAGATCT CCAGGGTAGA CTTCCGTGAC TTCCAGATGG TCGCACTGGA TCCAAAGGGA 
ACATCGCAGA TCGACCCCAA CTGGGTCATC AGGAACCAAG GGAAGGAGCT GGTGCAGACA 
GTCAACAGTG ATCCGGGCAT GGCCATAGGA GTTGACAGGT TCAACGAAGT GGACTTTGAC 
GGCACCTTCT ACGTGAACAC ACAAAAGGAC GACGACTACG CGGGCTTCGT GTTCGGCTAC 
CAGTCCAGCC ATCGCTTCTA CGTGGTGATG TGGAAACAGG TCCACCAGAC GTACTGGCTC 
TCCAAACCTT CCATGGCCCA CGGCTCAGCA GGAGTCCAAG TCAAGGTGGT AAACTCGGAG 
ACAGGACCAG GAGAGGCCCT GAGGAATGCC CTGTGGCACA CTGGAGACAC TCCCGGACAG 
GTGAGACTGC TCTGGCACGA CAAGAACACC ATTGGATGGA AAGACATGAC TGCCTACAGA 
TGGAAGCTGA AGCATAAACC AGACTCTGGA CTAATAAGGG TGACGTTCTA TGAAGGTAGT 
AAAGTTATGG CCGACTCTGG GGATCTATTC GACACAACAT TAAAGGGAGG TCGCCTAGGA 
CTCTTCGTCT TTTCACAAGA GATGGTCTTC TTCTCCGATC TGAAGTACAA ATGTGGTCAG 
CAAGAGGCCT TGAATTGA