tmc-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	tmc-1
Entrez Gene ID	188483
Full Name	Transmembrane channel-like protein 1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_075820.5	NP_508221.3	Transmembrane channel-like protein 1
NM_075820.6	NP_508221.3	Transmembrane channel-like protein 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ectopic expression of TMC-1 confers alkaline sensitivity to alkali-insensitive cells
Title: TMC-1 Mediates Alkaline Sensation in C.?elegans through Nociceptive Neurons.

TMC-1 has a role in regulating C. elegans development and sexual behaviour in a chemically defined food environment
Title: TMC-1 attenuates C. elegans development and sexual behaviour in a chemically defined food environment.

TMC-1 is both necessary for salt sensation in vivo and sufficient to generate a sodium-sensitive channel in vitro, identifying it as a probable ionotropic sensory receptor
Title: tmc-1 encodes a sodium-sensitive channel required for salt chemosensation in C. elegans.

The following tmc-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmc-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa349572	NM_075820.6 Latest version!	Caenorhabditis elegans Transmembrane channel-like protein 1 (tmc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa349572	NM_075820.5	Caenorhabditis elegans Transmembrane channel-like protein 1 (tmc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tmc-1 ( NM_075820.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_508221.3 Caenorhabditis elegans Transmembrane channel-like protein 1 (tmc-1), partial mRNA.

Price & Availability↑

CloneID	OCa349572
Clone ID Related Accession (Same CDS sequence)	NM_075820.5 , NM_075820.6
Accession Version	NM_075820.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Transmembrane channel-like protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_075820.4. COMPLETENESS: incomplete on both ends.

ATGCAGGAAG CGGCAAGGAG AGCGTCGCTA CGCAAGGAGC ACACGCCAAC AAATGAAAAG 
TTTGGAGATT TGTCAAAGCA GGATTCGTTG GGAGAAAGAG CGTCTTCAAA ACTCACCTTG 
GATGATGAAC TCTATGACAT TCTCTACGCG TTTGGTGAAA CCGATGCATT CATCAACAAG 
GGTGATAAGC AACGGGAAAC TGACGAGGAC GGGAATCCAC TGACACGGCA GGCACTTCTC 
GAGCGTATTC GGCAGAAAAA GGAGGTTATT GGAAAACTTC GGTGTCAGGC GTGGAGTATG 
ACAAGGAAGC GGCGGACATT GAAACTGGCT CAAAAATATC TGGAACAACA CGAGTCAAAA 
GTTTCGCGAA GTCATCTGTA CATGGAAGAG ATGAGAAAAA GGGCGAGGCT TATGAAACGG 
AGTTTTTCAA ATTTTAAAAC TTACCTCATA CCTTGGGAGT CGAAAATCAA ACGGATAGAA 
AGTCACTTCG GATCAGTTGT TTCGTCATAT TTCACATTTT TGCGATGGAT TGTTTTTGTG 
AACATAATGA TCACTTTGAT AGCTCTTGTT TTTGTGGTTT TACCAGAGAC CCTTGCTGAC 
TCTGTAGCAA ATGAAGGCCG TTTCAACCGA ACGAAAACGC GGAAACAAAT TCCAGCCAAC 
GAAAGAGTTC ATGCAGATGA GTTAGCTGTT GTTTGGCATT ATGATGGATA CCTTCGTTAC 
TCACCATTAT TTTATGGGTA CTACTCCGAC GACCCTTTTC TAGGAAACAA GATTAAATAT 
GCTCTACCAC TTGCTTATTT CATGGTGACA CTTACAATAT TCGCTTACAG TTTCTTTGCA 
ATCTTGAGGA AAATGGCTGC CAACGCTAGA ATGTCAAAGT TATCTGGTAG TAAAGCGGAA 
CAGTATATAT TCAATTGGAA ATTGTTCACC GGATGGGATT ATACCATTGG AAACTCGGAA 
ACTGCTAGTA ACACCGTGAT GGCTGTAGTG ATTAAGCTTA GGGAATCAAT TGCCGATATC 
AAAAAAGATG CACATGGAAA ATTTCGACTA CTGCAATTCT CTTTAAGAGT ATTCGCAAAC 
ATCATAATTT GTGCGATGCT TGGCTTCTCA ATCTACTGCA TCATATTCGC AGTGCAAAAA 
TCCCAAGTGC AAGACGACGG AAATCTATTC ACAAAAAATC AAGTTCCATC TGTCGTCTCG 
ACAATTACAC ACGTCTTTCC CATGATTTTC GATTTGATCG GAAAAATGGA AAATTATCAT 
CCGAGAACCG CGTTGAGAGC TCATTTGGGC AGAGTCCTAA TTTTGTATAC TGTAAACTAC 
ATCACTTTGA TATTTGCATT GTTCGAAAAA ATGACCGCAC TTCGAGATCG TGTCAATTCA 
ACTTCCACAT CGTCTAGTCA CAGAACGAAA AGACAGCAGG GTGGATGGAA TCCGAATATG 
CAACGACCAC CGCCATATGC TAGCCGAGCA GAAGTCCGCC AAATGAGTGA TTTTCTAGCT 
GCGAACACCA GAAGGTTCCA AACTGTTAGC CAACGTACAA CTCGTTCAGT GACTACACCT 
TTCACTGTTG CACCTCAATT TGGCCCATTT AATGTGAACA ATCCCAATGC AGTATTTCAC 
AACGGAACTC ATTCTACCAG TTTTGAATCG CAGATTCTTG GACCTAAAGC GTTACCGATA 
TTCACACCAC CACCGCGGAA ATATCCAGGA TTTACGCCGG GAAATGTTGG ACAGCAATTC 
GGAGGCCCTG ACTTTCCTAG AAACCAAGTA TACACAAAAT CGACTCCATT GCCACGTGTT 
CGAACAAAAC CACCTTGGGT ATACACGACG ACGCATCCAC CGTTAGTTCA GAATCGTGCA 
ATGACTACAA CAATGAGCAA ATCAGCGAAG AAAGGGAACT CAAAAAATTT AGATGATGAT 
ATATTGCTTT CGAACGAGAC CATTCAAATG TCTGAAGCAG CTCTTCGCCG AAACCACGAC 
GGGCATAACA ATGACATATG TTGGGAAACC ATCATTGGTC AGGAAATCGT CAAACTCGTT 
ACTATGGATC TCATTTTTAC GATTCTCTCC ATCCTAGTGA TCGACTTGTT TCGCGGCTTA 
TGGATCAAAT ACTGCTCGTC ATGGTGGTGC TGGGATATTG AGACGACGTT TCCCGAGTAT 
GGAGAGTTCA AGGTGGCGGA GAATGTACTG CACATCATCA ATAATCAGGG CATGATTTGG 
CTGGGATTGT TCTTTGCGCC CTTGCTTCCA GCGATTAACA ATATCAAGTT GATTATTTTG 
ATGTACATTC GAGGATGGGC AGTGATGACT TGCAATGTTC CAGCGAGGGA GATTTTCAGA 
GCAAGCAGAT CAAGCAACTT CTACCTTGGA ATTCTTCTCA TCTGGCTGCT CCTCTGTACT 
CTTCCAGTTG GCTTTGTCAT AGCCTCAATG AGCCCGTCCC GGTCGTGTGG CCCCTTTGCT 
CGATATCAAC ATTTCTACAC TGTGGTAACT AGAGAAATCG AGAAAAGAGT CGACCAAACA 
GTTCTGTCCT ACATTCGACA CATTGCTTCA CCCGGAGTTG TTATTCCTAT TATATTGTTT 
CTGATCCTCA TAATTTATTT CCTCTTTTCT CTGGTAAGAG GTCTCCGGGA AGCAAACACC 
GATCTTCAAG CTCAACTTGT GCACGAACGA ACTGAAGAGA AGAAGAAAAT CTTCGAATTG 
GCTGGTGGAA AGAAAAATAA GTTTGAAAAA GATCGAGATA AGAAACGATC AAACGACTAT 
ATTCCACTGA TTGAACAGCG ACGACGTGAG CCATGGAGGC AGTACCATGA GATGGAAGCG 
GATCACGCTT TGGCATCAGA TTCATCTGAA GAAAGTGACA TCAACGAGGA TGAAGACGAC 
GAACGCCAAC CGCTGACTGC ATATCCATTA AGAGCAATTG AAACACCACC AGAGACACTT 
CAAGTCACTG CGTTCCATCC ATCACTAGGA TCTCTAATCG AAAATCGAGA AATGGAAGAT 
GAAGAAAGTG CAAGTGGGGA CCAACTACCA ATGATTCACA AATCAGTGTC TTTCCAAGGA 
CCCTCTCATA TGCAAATGCG GCAGAGTATC TCAACAGAAA GTTGTAGTCA AATATCAAGG 
AGTGCAATTC AAGTGGCGAC GCCTGAAGAA ATACGAGCAT TGCTCCGACC ATATCTTGAA 
GCCAAGTATG GCATACCGTA TCAGCATGGA ATCAAATCTT TTCCCATTGA CGTGCACACC 
CCTCCAAATA ACACACCGTC CAGGAGATCG TCAAAGTATA ATTCGTTTGT GTCACTTTAT 
GAGCATACGA GAGATGATCA CAAAAACTTC GTTGCGTCGA CAATTAAAGA AACCGACGAA 
GACCCTGGCA AATCTGACAA GAAACAAACA TCCAGTAAAG ATGTGGCTCC CGATTTTATG 
CCTTGGCCGT CTGCAGATGA GGCACGAGCT CTACGAGAAA AGATGAAATC CAAAACTCCG 
CTAATGCTCA CAAAAACAAC TGTGGAGGAA AAACCGAAAG GCGGAAAATC TTCAGAATCG 
GAATTCCGAC CGCCTGTACC AATTCATAGA AAATATAATA TTCAGACAAC GGAGGAAGAA 
AACGAGGAGG AGGAGACAGA CTCAGCTCCG GAATCTTCCA AAAAACGCTT TAGAATTTCC 
GTGTCGCCGA CGAAGACTAT AGCTCCAGCA TCTGCAAGCA GAGCTCAACA TAAAATCGTA 
TCACAAGCAT CGTCTAGCAG CTCAATTCCA CATGGACGGC AACCGGATCC AAACAAGAAA 
GCGTCGCTTG TTTTGCCGCC GTTAAGAGCC CCACGTGTTC AGTTTGATGA GGATGACTCT 
CCGCGGCAAA TTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_508221.3
CDS	1..3858
Translation	MQEAARRASLRKEHTPTNEKFGDLSKQDSLGERASSKLTLDDELYDILYAFGETDAFINKGDKQRETDEDGNPLTRQALLERIRQKKEVIGKLRCQAWSMTRKRRTLKLAQKYLEQHESKVSRSHLYMEEMRKRARLMKRSFSNFKTYLIPWESKIKRIESHFGSVVSSYFTFLRWIVFVNIMITLIALVFVVLPETLADSVANEGRFNRTKTRKQIPANERVHADELAVVWHYDGYLRYSPLFYGYYSDDPFLGNKIKYALPLAYFMVTLTIFAYSFFAILRKMAANARMSKLSGSKAEQYIFNWKLFTGWDYTIGNSETASNTVMAVVIKLRESIADIKKDAHGKFRLLQFSLRVFANIIICAMLGFSIYCIIFAVQKSQVQDDGNLFTKNQVPSVVSTITHVFPMIFDLIGKMENYHPRTALRAHLGRVLILYTVNYITLIFALFEKMTALRDRVNSTSTSSSHRTKRQQGGWNPNMQRPPPYASRAEVRQMSDFLAANTRRFQTVSQRTTRSVTTPFTVAPQFGPFNVNNPNAVFHNGTHSTSFESQILGPKALPIFTPPPRKYPGFTPGNVGQQFGGPDFPRNQVYTKSTPLPRVRTKPPWVYTTTHPPLVQNRAMTTTMSKSAKKGNSKNLDDDILLSNETIQMSEAALRRNHDGHNNDICWETIIGQEIVKLVTMDLIFTILSILVIDLFRGLWIKYCSSWWCWDIETTFPEYGEFKVAENVLHIINNQGMIWLGLFFAPLLPAINNIKLIILMYIRGWAVMTCNVPAREIFRASRSSNFYLGILLIWLLLCTLPVGFVIASMSPSRSCGPFARYQHFYTVVTREIEKRVDQTVLSYIRHIASPGVVIPIILFLILIIYFLFSLVRGLREANTDLQAQLVHERTEEKKKIFELAGGKKNKFEKDRDKKRSNDYIPLIEQRRREPWRQYHEMEADHALASDSSEESDINEDEDDERQPLTAYPLRAIETPPETLQVTAFHPSLGSLIENREMEDEESASGDQLPMIHKSVSFQGPSHMQMRQSISTESCSQISRSAIQVATPEEIRALLRPYLEAKYGIPYQHGIKSFPIDVHTPPNNTPSRRSSKYNSFVSLYEHTRDDHKNFVASTIKETDEDPGKSDKKQTSSKDVAPDFMPWPSADEARALREKMKSKTPLMLTKTTVEEKPKGGKSSESEFRPPVPIHRKYNIQTTEEENEEEETDSAPESSKKRFRISVSPTKTIAPASASRAQHKIVSQASSSSSIPHGRQPDPNKKASLVLPPLRAPRVQFDEDDSPRQID

Target ORF information:

RefSeq Version	NM_075820.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Transmembrane channel-like protein 1 (tmc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_075820.5

ORF Insert Sequence:

ATGCAGGAAG CGGCAAGGAG AGCGTCGCTA CGCAAGGAGC ACACGCCAAC AAATGAAAAG 
TTTGGAGATT TGTCAAAGCA GGATTCGTTG GGAGAAAGAG CGTCTTCAAA ACTCACCTTG 
GATGATGAAC TCTATGACAT TCTCTACGCG TTTGGTGAAA CCGATGCATT CATCAACAAG 
GGTGATAAGC AACGGGAAAC TGACGAGGAC GGGAATCCAC TGACACGGCA GGCACTTCTC 
GAGCGTATTC GGCAGAAAAA GGAGGTTATT GGAAAACTTC GGTGTCAGGC GTGGAGTATG 
ACAAGGAAGC GGCGGACATT GAAACTGGCT CAAAAATATC TGGAACAACA CGAGTCAAAA 
GTTTCGCGAA GTCATCTGTA CATGGAAGAG ATGAGAAAAA GGGCGAGGCT TATGAAACGG 
AGTTTTTCAA ATTTTAAAAC TTACCTCATA CCTTGGGAGT CGAAAATCAA ACGGATAGAA 
AGTCACTTCG GATCAGTTGT TTCGTCATAT TTCACATTTT TGCGATGGAT TGTTTTTGTG 
AACATAATGA TCACTTTGAT AGCTCTTGTT TTTGTGGTTT TACCAGAGAC CCTTGCTGAC 
TCTGTAGCAA ATGAAGGCCG TTTCAACCGA ACGAAAACGC GGAAACAAAT TCCAGCCAAC 
GAAAGAGTTC ATGCAGATGA GTTAGCTGTT GTTTGGCATT ATGATGGATA CCTTCGTTAC 
TCACCATTAT TTTATGGGTA CTACTCCGAC GACCCTTTTC TAGGAAACAA GATTAAATAT 
GCTCTACCAC TTGCTTATTT CATGGTGACA CTTACAATAT TCGCTTACAG TTTCTTTGCA 
ATCTTGAGGA AAATGGCTGC CAACGCTAGA ATGTCAAAGT TATCTGGTAG TAAAGCGGAA 
CAGTATATAT TCAATTGGAA ATTGTTCACC GGATGGGATT ATACCATTGG AAACTCGGAA 
ACTGCTAGTA ACACCGTGAT GGCTGTAGTG ATTAAGCTTA GGGAATCAAT TGCCGATATC 
AAAAAAGATG CACATGGAAA ATTTCGACTA CTGCAATTCT CTTTAAGAGT ATTCGCAAAC 
ATCATAATTT GTGCGATGCT TGGCTTCTCA ATCTACTGCA TCATATTCGC AGTGCAAAAA 
TCCCAAGTGC AAGACGACGG AAATCTATTC ACAAAAAATC AAGTTCCATC TGTCGTCTCG 
ACAATTACAC ACGTCTTTCC CATGATTTTC GATTTGATCG GAAAAATGGA AAATTATCAT 
CCGAGAACCG CGTTGAGAGC TCATTTGGGC AGAGTCCTAA TTTTGTATAC TGTAAACTAC 
ATCACTTTGA TATTTGCATT GTTCGAAAAA ATGACCGCAC TTCGAGATCG TGTCAATTCA 
ACTTCCACAT CGTCTAGTCA CAGAACGAAA AGACAGCAGG GTGGATGGAA TCCGAATATG 
CAACGACCAC CGCCATATGC TAGCCGAGCA GAAGTCCGCC AAATGAGTGA TTTTCTAGCT 
GCGAACACCA GAAGGTTCCA AACTGTTAGC CAACGTACAA CTCGTTCAGT GACTACACCT 
TTCACTGTTG CACCTCAATT TGGCCCATTT AATGTGAACA ATCCCAATGC AGTATTTCAC 
AACGGAACTC ATTCTACCAG TTTTGAATCG CAGATTCTTG GACCTAAAGC GTTACCGATA 
TTCACACCAC CACCGCGGAA ATATCCAGGA TTTACGCCGG GAAATGTTGG ACAGCAATTC 
GGAGGCCCTG ACTTTCCTAG AAACCAAGTA TACACAAAAT CGACTCCATT GCCACGTGTT 
CGAACAAAAC CACCTTGGGT ATACACGACG ACGCATCCAC CGTTAGTTCA GAATCGTGCA 
ATGACTACAA CAATGAGCAA ATCAGCGAAG AAAGGGAACT CAAAAAATTT AGATGATGAT 
ATATTGCTTT CGAACGAGAC CATTCAAATG TCTGAAGCAG CTCTTCGCCG AAACCACGAC 
GGGCATAACA ATGACATATG TTGGGAAACC ATCATTGGTC AGGAAATCGT CAAACTCGTT 
ACTATGGATC TCATTTTTAC GATTCTCTCC ATCCTAGTGA TCGACTTGTT TCGCGGCTTA 
TGGATCAAAT ACTGCTCGTC ATGGTGGTGC TGGGATATTG AGACGACGTT TCCCGAGTAT 
GGAGAGTTCA AGGTGGCGGA GAATGTACTG CACATCATCA ATAATCAGGG CATGATTTGG 
CTGGGATTGT TCTTTGCGCC CTTGCTTCCA GCGATTAACA ATATCAAGTT GATTATTTTG 
ATGTACATTC GAGGATGGGC AGTGATGACT TGCAATGTTC CAGCGAGGGA GATTTTCAGA 
GCAAGCAGAT CAAGCAACTT CTACCTTGGA ATTCTTCTCA TCTGGCTGCT CCTCTGTACT 
CTTCCAGTTG GCTTTGTCAT AGCCTCAATG AGCCCGTCCC GGTCGTGTGG CCCCTTTGCT 
CGATATCAAC ATTTCTACAC TGTGGTAACT AGAGAAATCG AGAAAAGAGT CGACCAAACA 
GTTCTGTCCT ACATTCGACA CATTGCTTCA CCCGGAGTTG TTATTCCTAT TATATTGTTT 
CTGATCCTCA TAATTTATTT CCTCTTTTCT CTGGTAAGAG GTCTCCGGGA AGCAAACACC 
GATCTTCAAG CTCAACTTGT GCACGAACGA ACTGAAGAGA AGAAGAAAAT CTTCGAATTG 
GCTGGTGGAA AGAAAAATAA GTTTGAAAAA GATCGAGATA AGAAACGATC AAACGACTAT 
ATTCCACTGA TTGAACAGCG ACGACGTGAG CCATGGAGGC AGTACCATGA GATGGAAGCG 
GATCACGCTT TGGCATCAGA TTCATCTGAA GAAAGTGACA TCAACGAGGA TGAAGACGAC 
GAACGCCAAC CGCTGACTGC ATATCCATTA AGAGCAATTG AAACACCACC AGAGACACTT 
CAAGTCACTG CGTTCCATCC ATCACTAGGA TCTCTAATCG AAAATCGAGA AATGGAAGAT 
GAAGAAAGTG CAAGTGGGGA CCAACTACCA ATGATTCACA AATCAGTGTC TTTCCAAGGA 
CCCTCTCATA TGCAAATGCG GCAGAGTATC TCAACAGAAA GTTGTAGTCA AATATCAAGG 
AGTGCAATTC AAGTGGCGAC GCCTGAAGAA ATACGAGCAT TGCTCCGACC ATATCTTGAA 
GCCAAGTATG GCATACCGTA TCAGCATGGA ATCAAATCTT TTCCCATTGA CGTGCACACC 
CCTCCAAATA ACACACCGTC CAGGAGATCG TCAAAGTATA ATTCGTTTGT GTCACTTTAT 
GAGCATACGA GAGATGATCA CAAAAACTTC GTTGCGTCGA CAATTAAAGA AACCGACGAA 
GACCCTGGCA AATCTGACAA GAAACAAACA TCCAGTAAAG ATGTGGCTCC CGATTTTATG 
CCTTGGCCGT CTGCAGATGA GGCACGAGCT CTACGAGAAA AGATGAAATC CAAAACTCCG 
CTAATGCTCA CAAAAACAAC TGTGGAGGAA AAACCGAAAG GCGGAAAATC TTCAGAATCG 
GAATTCCGAC CGCCTGTACC AATTCATAGA AAATATAATA TTCAGACAAC GGAGGAAGAA 
AACGAGGAGG AGGAGACAGA CTCAGCTCCG GAATCTTCCA AAAAACGCTT TAGAATTTCC 
GTGTCGCCGA CGAAGACTAT AGCTCCAGCA TCTGCAAGCA GAGCTCAACA TAAAATCGTA 
TCACAAGCAT CGTCTAGCAG CTCAATTCCA CATGGACGGC AACCGGATCC AAACAAGAAA 
GCGTCGCTTG TTTTGCCGCC GTTAAGAGCC CCACGTGTTC AGTTTGATGA GGATGACTCT 
CCGCGGCAAA TTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmc-1 ( NM_075820.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_508221.3 Caenorhabditis elegans Transmembrane channel-like protein 1 (tmc-1), mRNA.

Price & Availability↑

CloneID	OCa349572
Clone ID Related Accession (Same CDS sequence)	NM_075820.5 , NM_075820.6
Accession Version	NM_075820.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Transmembrane channel-like protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_075820.5.

ATGCAGGAAG CGGCAAGGAG AGCGTCGCTA CGCAAGGAGC ACACGCCAAC AAATGAAAAG 
TTTGGAGATT TGTCAAAGCA GGATTCGTTG GGAGAAAGAG CGTCTTCAAA ACTCACCTTG 
GATGATGAAC TCTATGACAT TCTCTACGCG TTTGGTGAAA CCGATGCATT CATCAACAAG 
GGTGATAAGC AACGGGAAAC TGACGAGGAC GGGAATCCAC TGACACGGCA GGCACTTCTC 
GAGCGTATTC GGCAGAAAAA GGAGGTTATT GGAAAACTTC GGTGTCAGGC GTGGAGTATG 
ACAAGGAAGC GGCGGACATT GAAACTGGCT CAAAAATATC TGGAACAACA CGAGTCAAAA 
GTTTCGCGAA GTCATCTGTA CATGGAAGAG ATGAGAAAAA GGGCGAGGCT TATGAAACGG 
AGTTTTTCAA ATTTTAAAAC TTACCTCATA CCTTGGGAGT CGAAAATCAA ACGGATAGAA 
AGTCACTTCG GATCAGTTGT TTCGTCATAT TTCACATTTT TGCGATGGAT TGTTTTTGTG 
AACATAATGA TCACTTTGAT AGCTCTTGTT TTTGTGGTTT TACCAGAGAC CCTTGCTGAC 
TCTGTAGCAA ATGAAGGCCG TTTCAACCGA ACGAAAACGC GGAAACAAAT TCCAGCCAAC 
GAAAGAGTTC ATGCAGATGA GTTAGCTGTT GTTTGGCATT ATGATGGATA CCTTCGTTAC 
TCACCATTAT TTTATGGGTA CTACTCCGAC GACCCTTTTC TAGGAAACAA GATTAAATAT 
GCTCTACCAC TTGCTTATTT CATGGTGACA CTTACAATAT TCGCTTACAG TTTCTTTGCA 
ATCTTGAGGA AAATGGCTGC CAACGCTAGA ATGTCAAAGT TATCTGGTAG TAAAGCGGAA 
CAGTATATAT TCAATTGGAA ATTGTTCACC GGATGGGATT ATACCATTGG AAACTCGGAA 
ACTGCTAGTA ACACCGTGAT GGCTGTAGTG ATTAAGCTTA GGGAATCAAT TGCCGATATC 
AAAAAAGATG CACATGGAAA ATTTCGACTA CTGCAATTCT CTTTAAGAGT ATTCGCAAAC 
ATCATAATTT GTGCGATGCT TGGCTTCTCA ATCTACTGCA TCATATTCGC AGTGCAAAAA 
TCCCAAGTGC AAGACGACGG AAATCTATTC ACAAAAAATC AAGTTCCATC TGTCGTCTCG 
ACAATTACAC ACGTCTTTCC CATGATTTTC GATTTGATCG GAAAAATGGA AAATTATCAT 
CCGAGAACCG CGTTGAGAGC TCATTTGGGC AGAGTCCTAA TTTTGTATAC TGTAAACTAC 
ATCACTTTGA TATTTGCATT GTTCGAAAAA ATGACCGCAC TTCGAGATCG TGTCAATTCA 
ACTTCCACAT CGTCTAGTCA CAGAACGAAA AGACAGCAGG GTGGATGGAA TCCGAATATG 
CAACGACCAC CGCCATATGC TAGCCGAGCA GAAGTCCGCC AAATGAGTGA TTTTCTAGCT 
GCGAACACCA GAAGGTTCCA AACTGTTAGC CAACGTACAA CTCGTTCAGT GACTACACCT 
TTCACTGTTG CACCTCAATT TGGCCCATTT AATGTGAACA ATCCCAATGC AGTATTTCAC 
AACGGAACTC ATTCTACCAG TTTTGAATCG CAGATTCTTG GACCTAAAGC GTTACCGATA 
TTCACACCAC CACCGCGGAA ATATCCAGGA TTTACGCCGG GAAATGTTGG ACAGCAATTC 
GGAGGCCCTG ACTTTCCTAG AAACCAAGTA TACACAAAAT CGACTCCATT GCCACGTGTT 
CGAACAAAAC CACCTTGGGT ATACACGACG ACGCATCCAC CGTTAGTTCA GAATCGTGCA 
ATGACTACAA CAATGAGCAA ATCAGCGAAG AAAGGGAACT CAAAAAATTT AGATGATGAT 
ATATTGCTTT CGAACGAGAC CATTCAAATG TCTGAAGCAG CTCTTCGCCG AAACCACGAC 
GGGCATAACA ATGACATATG TTGGGAAACC ATCATTGGTC AGGAAATCGT CAAACTCGTT 
ACTATGGATC TCATTTTTAC GATTCTCTCC ATCCTAGTGA TCGACTTGTT TCGCGGCTTA 
TGGATCAAAT ACTGCTCGTC ATGGTGGTGC TGGGATATTG AGACGACGTT TCCCGAGTAT 
GGAGAGTTCA AGGTGGCGGA GAATGTACTG CACATCATCA ATAATCAGGG CATGATTTGG 
CTGGGATTGT TCTTTGCGCC CTTGCTTCCA GCGATTAACA ATATCAAGTT GATTATTTTG 
ATGTACATTC GAGGATGGGC AGTGATGACT TGCAATGTTC CAGCGAGGGA GATTTTCAGA 
GCAAGCAGAT CAAGCAACTT CTACCTTGGA ATTCTTCTCA TCTGGCTGCT CCTCTGTACT 
CTTCCAGTTG GCTTTGTCAT AGCCTCAATG AGCCCGTCCC GGTCGTGTGG CCCCTTTGCT 
CGATATCAAC ATTTCTACAC TGTGGTAACT AGAGAAATCG AGAAAAGAGT CGACCAAACA 
GTTCTGTCCT ACATTCGACA CATTGCTTCA CCCGGAGTTG TTATTCCTAT TATATTGTTT 
CTGATCCTCA TAATTTATTT CCTCTTTTCT CTGGTAAGAG GTCTCCGGGA AGCAAACACC 
GATCTTCAAG CTCAACTTGT GCACGAACGA ACTGAAGAGA AGAAGAAAAT CTTCGAATTG 
GCTGGTGGAA AGAAAAATAA GTTTGAAAAA GATCGAGATA AGAAACGATC AAACGACTAT 
ATTCCACTGA TTGAACAGCG ACGACGTGAG CCATGGAGGC AGTACCATGA GATGGAAGCG 
GATCACGCTT TGGCATCAGA TTCATCTGAA GAAAGTGACA TCAACGAGGA TGAAGACGAC 
GAACGCCAAC CGCTGACTGC ATATCCATTA AGAGCAATTG AAACACCACC AGAGACACTT 
CAAGTCACTG CGTTCCATCC ATCACTAGGA TCTCTAATCG AAAATCGAGA AATGGAAGAT 
GAAGAAAGTG CAAGTGGGGA CCAACTACCA ATGATTCACA AATCAGTGTC TTTCCAAGGA 
CCCTCTCATA TGCAAATGCG GCAGAGTATC TCAACAGAAA GTTGTAGTCA AATATCAAGG 
AGTGCAATTC AAGTGGCGAC GCCTGAAGAA ATACGAGCAT TGCTCCGACC ATATCTTGAA 
GCCAAGTATG GCATACCGTA TCAGCATGGA ATCAAATCTT TTCCCATTGA CGTGCACACC 
CCTCCAAATA ACACACCGTC CAGGAGATCG TCAAAGTATA ATTCGTTTGT GTCACTTTAT 
GAGCATACGA GAGATGATCA CAAAAACTTC GTTGCGTCGA CAATTAAAGA AACCGACGAA 
GACCCTGGCA AATCTGACAA GAAACAAACA TCCAGTAAAG ATGTGGCTCC CGATTTTATG 
CCTTGGCCGT CTGCAGATGA GGCACGAGCT CTACGAGAAA AGATGAAATC CAAAACTCCG 
CTAATGCTCA CAAAAACAAC TGTGGAGGAA AAACCGAAAG GCGGAAAATC TTCAGAATCG 
GAATTCCGAC CGCCTGTACC AATTCATAGA AAATATAATA TTCAGACAAC GGAGGAAGAA 
AACGAGGAGG AGGAGACAGA CTCAGCTCCG GAATCTTCCA AAAAACGCTT TAGAATTTCC 
GTGTCGCCGA CGAAGACTAT AGCTCCAGCA TCTGCAAGCA GAGCTCAACA TAAAATCGTA 
TCACAAGCAT CGTCTAGCAG CTCAATTCCA CATGGACGGC AACCGGATCC AAACAAGAAA 
GCGTCGCTTG TTTTGCCGCC GTTAAGAGCC CCACGTGTTC AGTTTGATGA GGATGACTCT 
CCGCGGCAAA TTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_508221.3
CDS	81..3938
Translation	MQEAARRASLRKEHTPTNEKFGDLSKQDSLGERASSKLTLDDELYDILYAFGETDAFINKGDKQRETDEDGNPLTRQALLERIRQKKEVIGKLRCQAWSMTRKRRTLKLAQKYLEQHESKVSRSHLYMEEMRKRARLMKRSFSNFKTYLIPWESKIKRIESHFGSVVSSYFTFLRWIVFVNIMITLIALVFVVLPETLADSVANEGRFNRTKTRKQIPANERVHADELAVVWHYDGYLRYSPLFYGYYSDDPFLGNKIKYALPLAYFMVTLTIFAYSFFAILRKMAANARMSKLSGSKAEQYIFNWKLFTGWDYTIGNSETASNTVMAVVIKLRESIADIKKDAHGKFRLLQFSLRVFANIIICAMLGFSIYCIIFAVQKSQVQDDGNLFTKNQVPSVVSTITHVFPMIFDLIGKMENYHPRTALRAHLGRVLILYTVNYITLIFALFEKMTALRDRVNSTSTSSSHRTKRQQGGWNPNMQRPPPYASRAEVRQMSDFLAANTRRFQTVSQRTTRSVTTPFTVAPQFGPFNVNNPNAVFHNGTHSTSFESQILGPKALPIFTPPPRKYPGFTPGNVGQQFGGPDFPRNQVYTKSTPLPRVRTKPPWVYTTTHPPLVQNRAMTTTMSKSAKKGNSKNLDDDILLSNETIQMSEAALRRNHDGHNNDICWETIIGQEIVKLVTMDLIFTILSILVIDLFRGLWIKYCSSWWCWDIETTFPEYGEFKVAENVLHIINNQGMIWLGLFFAPLLPAINNIKLIILMYIRGWAVMTCNVPAREIFRASRSSNFYLGILLIWLLLCTLPVGFVIASMSPSRSCGPFARYQHFYTVVTREIEKRVDQTVLSYIRHIASPGVVIPIILFLILIIYFLFSLVRGLREANTDLQAQLVHERTEEKKKIFELAGGKKNKFEKDRDKKRSNDYIPLIEQRRREPWRQYHEMEADHALASDSSEESDINEDEDDERQPLTAYPLRAIETPPETLQVTAFHPSLGSLIENREMEDEESASGDQLPMIHKSVSFQGPSHMQMRQSISTESCSQISRSAIQVATPEEIRALLRPYLEAKYGIPYQHGIKSFPIDVHTPPNNTPSRRSSKYNSFVSLYEHTRDDHKNFVASTIKETDEDPGKSDKKQTSSKDVAPDFMPWPSADEARALREKMKSKTPLMLTKTTVEEKPKGGKSSESEFRPPVPIHRKYNIQTTEEENEEEETDSAPESSKKRFRISVSPTKTIAPASASRAQHKIVSQASSSSSIPHGRQPDPNKKASLVLPPLRAPRVQFDEDDSPRQID

Target ORF information:

RefSeq Version	NM_075820.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Transmembrane channel-like protein 1 (tmc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_075820.6

ORF Insert Sequence:

ATGCAGGAAG CGGCAAGGAG AGCGTCGCTA CGCAAGGAGC ACACGCCAAC AAATGAAAAG 
TTTGGAGATT TGTCAAAGCA GGATTCGTTG GGAGAAAGAG CGTCTTCAAA ACTCACCTTG 
GATGATGAAC TCTATGACAT TCTCTACGCG TTTGGTGAAA CCGATGCATT CATCAACAAG 
GGTGATAAGC AACGGGAAAC TGACGAGGAC GGGAATCCAC TGACACGGCA GGCACTTCTC 
GAGCGTATTC GGCAGAAAAA GGAGGTTATT GGAAAACTTC GGTGTCAGGC GTGGAGTATG 
ACAAGGAAGC GGCGGACATT GAAACTGGCT CAAAAATATC TGGAACAACA CGAGTCAAAA 
GTTTCGCGAA GTCATCTGTA CATGGAAGAG ATGAGAAAAA GGGCGAGGCT TATGAAACGG 
AGTTTTTCAA ATTTTAAAAC TTACCTCATA CCTTGGGAGT CGAAAATCAA ACGGATAGAA 
AGTCACTTCG GATCAGTTGT TTCGTCATAT TTCACATTTT TGCGATGGAT TGTTTTTGTG 
AACATAATGA TCACTTTGAT AGCTCTTGTT TTTGTGGTTT TACCAGAGAC CCTTGCTGAC 
TCTGTAGCAA ATGAAGGCCG TTTCAACCGA ACGAAAACGC GGAAACAAAT TCCAGCCAAC 
GAAAGAGTTC ATGCAGATGA GTTAGCTGTT GTTTGGCATT ATGATGGATA CCTTCGTTAC 
TCACCATTAT TTTATGGGTA CTACTCCGAC GACCCTTTTC TAGGAAACAA GATTAAATAT 
GCTCTACCAC TTGCTTATTT CATGGTGACA CTTACAATAT TCGCTTACAG TTTCTTTGCA 
ATCTTGAGGA AAATGGCTGC CAACGCTAGA ATGTCAAAGT TATCTGGTAG TAAAGCGGAA 
CAGTATATAT TCAATTGGAA ATTGTTCACC GGATGGGATT ATACCATTGG AAACTCGGAA 
ACTGCTAGTA ACACCGTGAT GGCTGTAGTG ATTAAGCTTA GGGAATCAAT TGCCGATATC 
AAAAAAGATG CACATGGAAA ATTTCGACTA CTGCAATTCT CTTTAAGAGT ATTCGCAAAC 
ATCATAATTT GTGCGATGCT TGGCTTCTCA ATCTACTGCA TCATATTCGC AGTGCAAAAA 
TCCCAAGTGC AAGACGACGG AAATCTATTC ACAAAAAATC AAGTTCCATC TGTCGTCTCG 
ACAATTACAC ACGTCTTTCC CATGATTTTC GATTTGATCG GAAAAATGGA AAATTATCAT 
CCGAGAACCG CGTTGAGAGC TCATTTGGGC AGAGTCCTAA TTTTGTATAC TGTAAACTAC 
ATCACTTTGA TATTTGCATT GTTCGAAAAA ATGACCGCAC TTCGAGATCG TGTCAATTCA 
ACTTCCACAT CGTCTAGTCA CAGAACGAAA AGACAGCAGG GTGGATGGAA TCCGAATATG 
CAACGACCAC CGCCATATGC TAGCCGAGCA GAAGTCCGCC AAATGAGTGA TTTTCTAGCT 
GCGAACACCA GAAGGTTCCA AACTGTTAGC CAACGTACAA CTCGTTCAGT GACTACACCT 
TTCACTGTTG CACCTCAATT TGGCCCATTT AATGTGAACA ATCCCAATGC AGTATTTCAC 
AACGGAACTC ATTCTACCAG TTTTGAATCG CAGATTCTTG GACCTAAAGC GTTACCGATA 
TTCACACCAC CACCGCGGAA ATATCCAGGA TTTACGCCGG GAAATGTTGG ACAGCAATTC 
GGAGGCCCTG ACTTTCCTAG AAACCAAGTA TACACAAAAT CGACTCCATT GCCACGTGTT 
CGAACAAAAC CACCTTGGGT ATACACGACG ACGCATCCAC CGTTAGTTCA GAATCGTGCA 
ATGACTACAA CAATGAGCAA ATCAGCGAAG AAAGGGAACT CAAAAAATTT AGATGATGAT 
ATATTGCTTT CGAACGAGAC CATTCAAATG TCTGAAGCAG CTCTTCGCCG AAACCACGAC 
GGGCATAACA ATGACATATG TTGGGAAACC ATCATTGGTC AGGAAATCGT CAAACTCGTT 
ACTATGGATC TCATTTTTAC GATTCTCTCC ATCCTAGTGA TCGACTTGTT TCGCGGCTTA 
TGGATCAAAT ACTGCTCGTC ATGGTGGTGC TGGGATATTG AGACGACGTT TCCCGAGTAT 
GGAGAGTTCA AGGTGGCGGA GAATGTACTG CACATCATCA ATAATCAGGG CATGATTTGG 
CTGGGATTGT TCTTTGCGCC CTTGCTTCCA GCGATTAACA ATATCAAGTT GATTATTTTG 
ATGTACATTC GAGGATGGGC AGTGATGACT TGCAATGTTC CAGCGAGGGA GATTTTCAGA 
GCAAGCAGAT CAAGCAACTT CTACCTTGGA ATTCTTCTCA TCTGGCTGCT CCTCTGTACT 
CTTCCAGTTG GCTTTGTCAT AGCCTCAATG AGCCCGTCCC GGTCGTGTGG CCCCTTTGCT 
CGATATCAAC ATTTCTACAC TGTGGTAACT AGAGAAATCG AGAAAAGAGT CGACCAAACA 
GTTCTGTCCT ACATTCGACA CATTGCTTCA CCCGGAGTTG TTATTCCTAT TATATTGTTT 
CTGATCCTCA TAATTTATTT CCTCTTTTCT CTGGTAAGAG GTCTCCGGGA AGCAAACACC 
GATCTTCAAG CTCAACTTGT GCACGAACGA ACTGAAGAGA AGAAGAAAAT CTTCGAATTG 
GCTGGTGGAA AGAAAAATAA GTTTGAAAAA GATCGAGATA AGAAACGATC AAACGACTAT 
ATTCCACTGA TTGAACAGCG ACGACGTGAG CCATGGAGGC AGTACCATGA GATGGAAGCG 
GATCACGCTT TGGCATCAGA TTCATCTGAA GAAAGTGACA TCAACGAGGA TGAAGACGAC 
GAACGCCAAC CGCTGACTGC ATATCCATTA AGAGCAATTG AAACACCACC AGAGACACTT 
CAAGTCACTG CGTTCCATCC ATCACTAGGA TCTCTAATCG AAAATCGAGA AATGGAAGAT 
GAAGAAAGTG CAAGTGGGGA CCAACTACCA ATGATTCACA AATCAGTGTC TTTCCAAGGA 
CCCTCTCATA TGCAAATGCG GCAGAGTATC TCAACAGAAA GTTGTAGTCA AATATCAAGG 
AGTGCAATTC AAGTGGCGAC GCCTGAAGAA ATACGAGCAT TGCTCCGACC ATATCTTGAA 
GCCAAGTATG GCATACCGTA TCAGCATGGA ATCAAATCTT TTCCCATTGA CGTGCACACC 
CCTCCAAATA ACACACCGTC CAGGAGATCG TCAAAGTATA ATTCGTTTGT GTCACTTTAT 
GAGCATACGA GAGATGATCA CAAAAACTTC GTTGCGTCGA CAATTAAAGA AACCGACGAA 
GACCCTGGCA AATCTGACAA GAAACAAACA TCCAGTAAAG ATGTGGCTCC CGATTTTATG 
CCTTGGCCGT CTGCAGATGA GGCACGAGCT CTACGAGAAA AGATGAAATC CAAAACTCCG 
CTAATGCTCA CAAAAACAAC TGTGGAGGAA AAACCGAAAG GCGGAAAATC TTCAGAATCG 
GAATTCCGAC CGCCTGTACC AATTCATAGA AAATATAATA TTCAGACAAC GGAGGAAGAA 
AACGAGGAGG AGGAGACAGA CTCAGCTCCG GAATCTTCCA AAAAACGCTT TAGAATTTCC 
GTGTCGCCGA CGAAGACTAT AGCTCCAGCA TCTGCAAGCA GAGCTCAACA TAAAATCGTA 
TCACAAGCAT CGTCTAGCAG CTCAATTCCA CATGGACGGC AACCGGATCC AAACAAGAAA 
GCGTCGCTTG TTTTGCCGCC GTTAAGAGCC CCACGTGTTC AGTTTGATGA GGATGACTCT 
CCGCGGCAAA TTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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tmc-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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