hlh-17 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	hlh-17
Entrez Gene ID	185460
Full Name	Helix Loop Helix
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_070527.4	NP_502928.3	Helix Loop Helix
NM_070527.5	NP_502928.3	BHLH domain-containing protein

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	hlh-32	NP_001023430.1
Caenorhabditis elegans (roundworm)	hlh-17	NP_502928.3
Caenorhabditis elegans (roundworm)	hlh-31	NP_001023193.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our work suggests that HLH-17 is required to maintain normal levels of dopamine in the synaptic cleft through its regulation of dop-3 and dat-1.
Title: Dopamine signaling in C. elegans is mediated in part by HLH-17-dependent regulation of extracellular dopamine levels.

Caenorhabditis elegans locomotion phenotype caused by transgenic repeats of the hlh-17 promoter sequence
Title: A Caenorhabditis elegans locomotion phenotype caused by transgenic repeats of the hlh-17 promoter sequence.

HLH-17 has a role in dopamine signaling in Caenorhabditis elegans.
Title: Modulation of dopamine-dependent behaviors by the Caenorhabditis elegans Olig homolog HLH-17.

demonstrate ventral- and dorsal-restricted roles for the mls-2/Nkx/Hmx and vab-3/Pax6/Pax7 genes, respectively, in CEPsh glia differentiation and expression of the genes hlh-17/Olig and ptr-10/Patched-related
Title: mls-2 and vab-3 Control glia development, hlh-17/Olig expression and glia-dependent neurite extension in C. elegans.

It is proposed that hlh-17 affects the ability of C. elegans to respond to food cues, with possible downstream effects on insulin-signaling genes involved in the normal development and reproductive viability of the worm.
Title: Molecular characterization of HLH-17, a C. elegans bHLH protein required for normal larval development.

The following hlh-17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hlh-17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa342481	NM_070527.5 Latest version!	Caenorhabditis elegans BHLH domain-containing protein (hlh-17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OCa342481	NM_070527.4	Caenorhabditis elegans Helix Loop Helix (hlh-17), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hlh-17 ( NM_070527.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_502928.3 Caenorhabditis elegans Helix Loop Helix (hlh-17), partial mRNA.

Price & Availability↑

CloneID	OCa342481
Clone ID Related Accession (Same CDS sequence)	NM_070527.4 , NM_070527.5
Accession Version	NM_070527.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Helix Loop Helix
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_070527.3. COMPLETENESS: incomplete on both ends.

ATGGAGAAAA TTGGAATTGA TACTGCTTTC GCTGAGCCTG GTGTTCGACT TTCCATCAAT 
TTGAGAGAAA GATGCCGAAT GCATGACTTA AACGAGGCAC TTGACGATCT GCGAGCTGTA 
ATTCCATATG CTCACGGGGG TTCCGTGAGG AAGTTGAGCA AGATTGCTAC GTTGCTTTTG 
GCCAAAAATC ATATTATCAT GCAGGCAAAA GCAATTGAGG AGCTGAGTAT ATTGGTCTCC 
CAGTTGAAGC GGAAATCGGA AAATTTGGAG AACTTGAACA AATCCTTAAA GCCTGATGCA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_502928.3
CDS	1..306
Translation	MEKIGIDTAFAEPGVRLSINLRERCRMHDLNEALDDLRAVIPYAHGGSVRKLSKIATLLLAKNHIIMQAKAIEELSILVSQLKRKSENLENLNKSLKPDAN

Target ORF information:

RefSeq Version	NM_070527.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Helix Loop Helix (hlh-17), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_070527.4

ORF Insert Sequence:

ATGGAGAAAA TTGGAATTGA TACTGCTTTC GCTGAGCCTG GTGTTCGACT TTCCATCAAT 
TTGAGAGAAA GATGCCGAAT GCATGACTTA AACGAGGCAC TTGACGATCT GCGAGCTGTA 
ATTCCATATG CTCACGGGGG TTCCGTGAGG AAGTTGAGCA AGATTGCTAC GTTGCTTTTG 
GCCAAAAATC ATATTATCAT GCAGGCAAAA GCAATTGAGG AGCTGAGTAT ATTGGTCTCC 
CAGTTGAAGC GGAAATCGGA AAATTTGGAG AACTTGAACA AATCCTTAAA GCCTGATGCA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hlh-17 ( NM_070527.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_502928.3 Caenorhabditis elegans BHLH domain-containing protein (hlh-17), mRNA.

Price & Availability↑

CloneID	OCa342481
Clone ID Related Accession (Same CDS sequence)	NM_070527.4 , NM_070527.5
Accession Version	NM_070527.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	BHLH domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_070527.4.

ATGGAGAAAA TTGGAATTGA TACTGCTTTC GCTGAGCCTG GTGTTCGACT TTCCATCAAT 
TTGAGAGAAA GATGCCGAAT GCATGACTTA AACGAGGCAC TTGACGATCT GCGAGCTGTA 
ATTCCATATG CTCACGGGGG TTCCGTGAGG AAGTTGAGCA AGATTGCTAC GTTGCTTTTG 
GCCAAAAATC ATATTATCAT GCAGGCAAAA GCAATTGAGG AGCTGAGTAT ATTGGTCTCC 
CAGTTGAAGC GGAAATCGGA AAATTTGGAG AACTTGAACA AATCCTTAAA GCCTGATGCA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_502928.3
CDS	108..413
Translation	MEKIGIDTAFAEPGVRLSINLRERCRMHDLNEALDDLRAVIPYAHGGSVRKLSKIATLLLAKNHIIMQAKAIEELSILVSQLKRKSENLENLNKSLKPDAN

Target ORF information:

RefSeq Version	NM_070527.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans BHLH domain-containing protein (hlh-17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_070527.5

ORF Insert Sequence:

ATGGAGAAAA TTGGAATTGA TACTGCTTTC GCTGAGCCTG GTGTTCGACT TTCCATCAAT 
TTGAGAGAAA GATGCCGAAT GCATGACTTA AACGAGGCAC TTGACGATCT GCGAGCTGTA 
ATTCCATATG CTCACGGGGG TTCCGTGAGG AAGTTGAGCA AGATTGCTAC GTTGCTTTTG 
GCCAAAAATC ATATTATCAT GCAGGCAAAA GCAATTGAGG AGCTGAGTAT ATTGGTCTCC 
CAGTTGAAGC GGAAATCGGA AAATTTGGAG AACTTGAACA AATCCTTAAA GCCTGATGCA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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hlh-17 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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