pezo-1 cDNA ORF clone, Caenorhabditis elegans

The following pezo-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pezo-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa356224 NM_001306748.2
Latest version!
Caenorhabditis elegans Piezo_RRas_bdg domain-containing protein (pezo-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCa356221 NM_001372904.1
Latest version!
Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa356221 NM_001307053.1
Latest version!
Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa356222 NM_001268456.3
Latest version!
Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa356223 NM_001268457.3
Latest version!
Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa342180 NM_001268460.3
Latest version!
Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342181 NM_001268461.3
Latest version!
Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342182 NM_001268458.3
Latest version!
Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342183 NM_001268459.3
Latest version!
Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342184 NM_001268462.3
Latest version!
Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa343007 NM_001307050.2
Latest version!
Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa343008 NM_001307051.2
Latest version!
Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa343009 NM_001307052.2
Latest version!
Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
View Old Accession Versions >>
OCa343008 NM_001307051.1 Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa356224 NM_001306748.1 Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa356223 NM_001268457.2 Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa342181 NM_001268461.2 Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa343009 NM_001307052.1 Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342182 NM_001268458.2 Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342183 NM_001268459.2 Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342184 NM_001268462.2 Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa356222 NM_001268456.2 Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa342180 NM_001268460.2 Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa343007 NM_001307050.1 Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa356224
    Clone ID Related Accession (Same CDS sequence) NM_001306748.1 , NM_001306748.2
    Accession Version NM_001306748.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTCAAAT ACGATCCTGA AAATGATGAT CTTGTGGAAC CCGTCGACAG TTTTGTACCA 
    GAAGTTGATC CCAAGGCAAC TGCATATGAT CGACTTGATC CTGGACAGAT AATGTACGCT
    GCCACCGCTC ACGACCTCGA CCTCGCCAAA ACTGTACAGC AAGTCAAGAA AGGCGATACT
    ATCAAAGATC CTGATTCACG TGCTCTTATC GCTGTTTCTG AACCAGAAGC TCGTAAGCCA
    GGAGGCACTG AAGAAACGGA TGGTGATGAA GATGAGGATA ATAAGGATTC GAAAGTGGAA
    AGTACTGCTA AATTCATTCA AAAAATGATC GCTTCTGCCT TGGACTTGTG TAGTGTAACA
    CTAAACAAAC TGTGCAGAGA ACACCGATAT GTTGGATTTG TTTTGAGCAA GGAAAAACAA
    AAGTTGAAGT CAGGACACTC GGAATCTCTT TCAAATACAT CTAGAAAGCT GACGGATATT
    CGATCTGCAG TTGATCTACC AAGTCTTCAA CTTGTGCAAT CGGCGAATGA TGTTGAAAAG
    ATGGAGACAG CCGTCAGCGT GGATTGGCAA CAAAAATCAT CAGCAACGAG ACTATTGAAT
    GCAGTTGTGA ATTGTATTGG AGCACACACT GATATTCTCT GCTACTTCTT CGCAATTATG
    ACACAAGTGA TGACTGGTGG ACTTATTACT CTTCCACTTC CTCTGATGTC ACTATTCTGG
    GGAAATCTAT CGAATCCTCG TCCATCCAAG TTCTTCTGGG TCACAATGAT CACCTACACT
    GAGTGTGTCA TTGTTATCAA GTTTGTTTGC CAATTTGCAT TCATGCCTTA CAATTCTATC
    ACGTGGCGAA CAGAGCATCA AATGGACCCG ATGTCATTGG ATAAGTTATT CGGAGTATCA
    CAGAGAGATA GTTTTGCACT TTGGGACATT GTTTTGCTCT TCTCACTCTT CTTCCATCGG
    TATATGCTCA GAAAGTTAGG GTTATGGAAG GATGCCAATT TGACGGACAC GTTCACTTTG
    AAGGAAGAGC CTCGATCGGC GTCAGGATCG GACACAGGAT CACCCAAGAA AATTGCTCAA
    GAGCCAAAGG TTGTTGTAAC TCAGTCAGAT ACCCTTGAAG GTACAAGTGG AGGAGAGATA
    GTCATACCAT CTGATCCAAA TGCAGTTTCC AATATGGAAG AATTAGACTG CGAACCTCCG
    ATTCCAGAGA AGCAAAGTGG ACCAATTGGA AGATTCATTC ATCAATTATT CCATCCAAAA
    TTCCGTTACA TTCGTGATTT GTATCCAATA ATGTTTGGAA TCGATGTGAT ATGCTTCCTC
    ATCATGACAT TTGGATACTC GGCATTTGGT GAAGGAGGTT CTGGAAATGT TTTAGATGAT
    GTGAAGGCTT CTCGTATTCC GGTAACACTT GTTGTTATGC TCGTTGGAAT GACTCTGGCA
    ATTATCATTG ATCGAGCACT CTACCTCAGA AAATCAGTTG TCGGAAAGCT AATCTATCAA
    GTCTTAATGA TTGCTTTCCT TCACATTTGG GTATTCCTTG TTCTTCCAAA TATGACTCGT
    CGTTCTGCGA TATCGAATCA CGTTGCTCAA GCATTGTACG TCATCAAATC GTGCTATTTC
    CTTGTGTCTG CTTGGCAGAT CCGAAATGGT TATCCTGAAC TTTGTATTGG AAATCTATTA
    ACACATTCAT ACGGAATGAC CAATATGATT GCATTCAAAG TATTCATGAA TATTCCATTC
    CTCTTCGAGC TTCGTACAGC TATTGATTGG ACATGGACAG ATACTTCAAT GCCTCTTTTC
    GATTTCTTCA ATATGGAAAA TTTCTATGCT CATATATTCA ATATCAAGTG TGCCCGCCAG
    TTCGAAGCTG CCTATCCAGC TCCAAGAGGG ATTCCCAAAG GAAAGTTGGT CAAGTATATG
    ATGGGATTCC CAATTATCAT TGGAGTCGTT ATCTTCATCT TTTCTCCTCT ATTGCTCTGG
    TCGCTACTCA ATCAAATCGG AACTATCAGT ATGCCAGAAA AAGTTACGTT GAGGATTTCG
    ATTGAAGGAT ATCCACCACT TTACGAGATG GAGGCACAAG GAAGTAATCA CGATAACGCT
    GAACTTGGAA TGATAAAACC TGATCAATTG GCATCTTTGA ATCAAGCATT GACGGATAGT
    TACACGACAC GAGACACTAA TTCTATTTTA AGATCAAGAA TGTCTGTATC ATATCTGAAA
    GGATACACAT ATGAGGATAT TCTGATTGTG CGATTCCGTC CAGAATCTGA AATTTATTGG
    CCGATTTCAC AGGATTCGAG AAATGCGATG ATCGATAAAT TGAGCCGAAA CACGTCAGTC
    AATTTTGAAG TATCTCTGGA ATTCACTCGG CCATATGATC CAAATGAAAA CGCTGCTTTA
    AAACATTCGA AATCGTGGCT TGTTCCAATC TCCCTGGATA TGACGATTCG AGCAAAAATT
    CAAAGTGCTC TCCGAGGAGA TCCAGGTCAT CCGATTCTCA TTCCACAGTC AATTCCTGCG
    TTTATTCAAG TTCCAAATCA AGGAGAATTG ACCCTTCCAA CATCAATCGG AAATACTATT
    ATCAATGACG GAAATCCACG GATTAATACG ACCGGGATGG AAAAATCAGA CGAAGCTCGG
    GCTTGGTTCG ACTCGTTAAC CTTGAATCTC GAACAAGGAA AATCGCAAAA CGAGAAAATG
    TGGATCGCCA CATCCGAACA TCCAGGAGAC CAAAATGCGA AACTCTGGAT AAAGACTGCA
    AATACGACGT ATTCGGGAAG GCCGTACCTT CAAGTTGTCG GATTTATTGA TAGAGCATTC
    CCATCATTTT TGGCGAAAGT TTTCAAAGGA GGAGTCATTG CAGTGTATCT TTCAGTTATC
    CTCGTCGTTG GAAGAGGTCT GGTTCGTGGA ATCTTCACCA CATCTCCAAG CACTGTCATG
    TTCACAGAAT TACCGAATGC GGATCATCTT CTCAAAATTT GTCTCGACAT CTATTTGGTT
    CGAGAAGCGA AGGACTTCAT GTTGGAGCAG GATCTCTTCG CCAAATTAAT ATTCCTGTTC
    CGATCACCAG CGACACTCAT CGAATGGACA CGAATGAGCA AAAAGAAGCA AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293677.1
    CDS1..3117
    Translation

    Target ORF information:

    RefSeq Version NM_001306748.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTCAAAT ACGATCCTGA AAATGATGAT CTTGTGGAAC CCGTCGACAG TTTTGTACCA 
    GAAGTTGATC CCAAGGCAAC TGCATATGAT CGACTTGATC CTGGACAGAT AATGTACGCT
    GCCACCGCTC ACGACCTCGA CCTCGCCAAA ACTGTACAGC AAGTCAAGAA AGGCGATACT
    ATCAAAGATC CTGATTCACG TGCTCTTATC GCTGTTTCTG AACCAGAAGC TCGTAAGCCA
    GGAGGCACTG AAGAAACGGA TGGTGATGAA GATGAGGATA ATAAGGATTC GAAAGTGGAA
    AGTACTGCTA AATTCATTCA AAAAATGATC GCTTCTGCCT TGGACTTGTG TAGTGTAACA
    CTAAACAAAC TGTGCAGAGA ACACCGATAT GTTGGATTTG TTTTGAGCAA GGAAAAACAA
    AAGTTGAAGT CAGGACACTC GGAATCTCTT TCAAATACAT CTAGAAAGCT GACGGATATT
    CGATCTGCAG TTGATCTACC AAGTCTTCAA CTTGTGCAAT CGGCGAATGA TGTTGAAAAG
    ATGGAGACAG CCGTCAGCGT GGATTGGCAA CAAAAATCAT CAGCAACGAG ACTATTGAAT
    GCAGTTGTGA ATTGTATTGG AGCACACACT GATATTCTCT GCTACTTCTT CGCAATTATG
    ACACAAGTGA TGACTGGTGG ACTTATTACT CTTCCACTTC CTCTGATGTC ACTATTCTGG
    GGAAATCTAT CGAATCCTCG TCCATCCAAG TTCTTCTGGG TCACAATGAT CACCTACACT
    GAGTGTGTCA TTGTTATCAA GTTTGTTTGC CAATTTGCAT TCATGCCTTA CAATTCTATC
    ACGTGGCGAA CAGAGCATCA AATGGACCCG ATGTCATTGG ATAAGTTATT CGGAGTATCA
    CAGAGAGATA GTTTTGCACT TTGGGACATT GTTTTGCTCT TCTCACTCTT CTTCCATCGG
    TATATGCTCA GAAAGTTAGG GTTATGGAAG GATGCCAATT TGACGGACAC GTTCACTTTG
    AAGGAAGAGC CTCGATCGGC GTCAGGATCG GACACAGGAT CACCCAAGAA AATTGCTCAA
    GAGCCAAAGG TTGTTGTAAC TCAGTCAGAT ACCCTTGAAG GTACAAGTGG AGGAGAGATA
    GTCATACCAT CTGATCCAAA TGCAGTTTCC AATATGGAAG AATTAGACTG CGAACCTCCG
    ATTCCAGAGA AGCAAAGTGG ACCAATTGGA AGATTCATTC ATCAATTATT CCATCCAAAA
    TTCCGTTACA TTCGTGATTT GTATCCAATA ATGTTTGGAA TCGATGTGAT ATGCTTCCTC
    ATCATGACAT TTGGATACTC GGCATTTGGT GAAGGAGGTT CTGGAAATGT TTTAGATGAT
    GTGAAGGCTT CTCGTATTCC GGTAACACTT GTTGTTATGC TCGTTGGAAT GACTCTGGCA
    ATTATCATTG ATCGAGCACT CTACCTCAGA AAATCAGTTG TCGGAAAGCT AATCTATCAA
    GTCTTAATGA TTGCTTTCCT TCACATTTGG GTATTCCTTG TTCTTCCAAA TATGACTCGT
    CGTTCTGCGA TATCGAATCA CGTTGCTCAA GCATTGTACG TCATCAAATC GTGCTATTTC
    CTTGTGTCTG CTTGGCAGAT CCGAAATGGT TATCCTGAAC TTTGTATTGG AAATCTATTA
    ACACATTCAT ACGGAATGAC CAATATGATT GCATTCAAAG TATTCATGAA TATTCCATTC
    CTCTTCGAGC TTCGTACAGC TATTGATTGG ACATGGACAG ATACTTCAAT GCCTCTTTTC
    GATTTCTTCA ATATGGAAAA TTTCTATGCT CATATATTCA ATATCAAGTG TGCCCGCCAG
    TTCGAAGCTG CCTATCCAGC TCCAAGAGGG ATTCCCAAAG GAAAGTTGGT CAAGTATATG
    ATGGGATTCC CAATTATCAT TGGAGTCGTT ATCTTCATCT TTTCTCCTCT ATTGCTCTGG
    TCGCTACTCA ATCAAATCGG AACTATCAGT ATGCCAGAAA AAGTTACGTT GAGGATTTCG
    ATTGAAGGAT ATCCACCACT TTACGAGATG GAGGCACAAG GAAGTAATCA CGATAACGCT
    GAACTTGGAA TGATAAAACC TGATCAATTG GCATCTTTGA ATCAAGCATT GACGGATAGT
    TACACGACAC GAGACACTAA TTCTATTTTA AGATCAAGAA TGTCTGTATC ATATCTGAAA
    GGATACACAT ATGAGGATAT TCTGATTGTG CGATTCCGTC CAGAATCTGA AATTTATTGG
    CCGATTTCAC AGGATTCGAG AAATGCGATG ATCGATAAAT TGAGCCGAAA CACGTCAGTC
    AATTTTGAAG TATCTCTGGA ATTCACTCGG CCATATGATC CAAATGAAAA CGCTGCTTTA
    AAACATTCGA AATCGTGGCT TGTTCCAATC TCCCTGGATA TGACGATTCG AGCAAAAATT
    CAAAGTGCTC TCCGAGGAGA TCCAGGTCAT CCGATTCTCA TTCCACAGTC AATTCCTGCG
    TTTATTCAAG TTCCAAATCA AGGAGAATTG ACCCTTCCAA CATCAATCGG AAATACTATT
    ATCAATGACG GAAATCCACG GATTAATACG ACCGGGATGG AAAAATCAGA CGAAGCTCGG
    GCTTGGTTCG ACTCGTTAAC CTTGAATCTC GAACAAGGAA AATCGCAAAA CGAGAAAATG
    TGGATCGCCA CATCCGAACA TCCAGGAGAC CAAAATGCGA AACTCTGGAT AAAGACTGCA
    AATACGACGT ATTCGGGAAG GCCGTACCTT CAAGTTGTCG GATTTATTGA TAGAGCATTC
    CCATCATTTT TGGCGAAAGT TTTCAAAGGA GGAGTCATTG CAGTGTATCT TTCAGTTATC
    CTCGTCGTTG GAAGAGGTCT GGTTCGTGGA ATCTTCACCA CATCTCCAAG CACTGTCATG
    TTCACAGAAT TACCGAATGC GGATCATCTT CTCAAAATTT GTCTCGACAT CTATTTGGTT
    CGAGAAGCGA AGGACTTCAT GTTGGAGCAG GATCTCTTCG CCAAATTAAT ATTCCTGTTC
    CGATCACCAG CGACACTCAT CGAATGGACA CGAATGAGCA AAAAGAAGCA AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa356224
    Clone ID Related Accession (Same CDS sequence) NM_001306748.1 , NM_001306748.2
    Accession Version NM_001306748.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Piezo_RRas_bdg domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306748.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTCAAAT ACGATCCTGA AAATGATGAT CTTGTGGAAC CCGTCGACAG TTTTGTACCA 
    GAAGTTGATC CCAAGGCAAC TGCATATGAT CGACTTGATC CTGGACAGAT AATGTACGCT
    GCCACCGCTC ACGACCTCGA CCTCGCCAAA ACTGTACAGC AAGTCAAGAA AGGCGATACT
    ATCAAAGATC CTGATTCACG TGCTCTTATC GCTGTTTCTG AACCAGAAGC TCGTAAGCCA
    GGAGGCACTG AAGAAACGGA TGGTGATGAA GATGAGGATA ATAAGGATTC GAAAGTGGAA
    AGTACTGCTA AATTCATTCA AAAAATGATC GCTTCTGCCT TGGACTTGTG TAGTGTAACA
    CTAAACAAAC TGTGCAGAGA ACACCGATAT GTTGGATTTG TTTTGAGCAA GGAAAAACAA
    AAGTTGAAGT CAGGACACTC GGAATCTCTT TCAAATACAT CTAGAAAGCT GACGGATATT
    CGATCTGCAG TTGATCTACC AAGTCTTCAA CTTGTGCAAT CGGCGAATGA TGTTGAAAAG
    ATGGAGACAG CCGTCAGCGT GGATTGGCAA CAAAAATCAT CAGCAACGAG ACTATTGAAT
    GCAGTTGTGA ATTGTATTGG AGCACACACT GATATTCTCT GCTACTTCTT CGCAATTATG
    ACACAAGTGA TGACTGGTGG ACTTATTACT CTTCCACTTC CTCTGATGTC ACTATTCTGG
    GGAAATCTAT CGAATCCTCG TCCATCCAAG TTCTTCTGGG TCACAATGAT CACCTACACT
    GAGTGTGTCA TTGTTATCAA GTTTGTTTGC CAATTTGCAT TCATGCCTTA CAATTCTATC
    ACGTGGCGAA CAGAGCATCA AATGGACCCG ATGTCATTGG ATAAGTTATT CGGAGTATCA
    CAGAGAGATA GTTTTGCACT TTGGGACATT GTTTTGCTCT TCTCACTCTT CTTCCATCGG
    TATATGCTCA GAAAGTTAGG GTTATGGAAG GATGCCAATT TGACGGACAC GTTCACTTTG
    AAGGAAGAGC CTCGATCGGC GTCAGGATCG GACACAGGAT CACCCAAGAA AATTGCTCAA
    GAGCCAAAGG TTGTTGTAAC TCAGTCAGAT ACCCTTGAAG GTACAAGTGG AGGAGAGATA
    GTCATACCAT CTGATCCAAA TGCAGTTTCC AATATGGAAG AATTAGACTG CGAACCTCCG
    ATTCCAGAGA AGCAAAGTGG ACCAATTGGA AGATTCATTC ATCAATTATT CCATCCAAAA
    TTCCGTTACA TTCGTGATTT GTATCCAATA ATGTTTGGAA TCGATGTGAT ATGCTTCCTC
    ATCATGACAT TTGGATACTC GGCATTTGGT GAAGGAGGTT CTGGAAATGT TTTAGATGAT
    GTGAAGGCTT CTCGTATTCC GGTAACACTT GTTGTTATGC TCGTTGGAAT GACTCTGGCA
    ATTATCATTG ATCGAGCACT CTACCTCAGA AAATCAGTTG TCGGAAAGCT AATCTATCAA
    GTCTTAATGA TTGCTTTCCT TCACATTTGG GTATTCCTTG TTCTTCCAAA TATGACTCGT
    CGTTCTGCGA TATCGAATCA CGTTGCTCAA GCATTGTACG TCATCAAATC GTGCTATTTC
    CTTGTGTCTG CTTGGCAGAT CCGAAATGGT TATCCTGAAC TTTGTATTGG AAATCTATTA
    ACACATTCAT ACGGAATGAC CAATATGATT GCATTCAAAG TATTCATGAA TATTCCATTC
    CTCTTCGAGC TTCGTACAGC TATTGATTGG ACATGGACAG ATACTTCAAT GCCTCTTTTC
    GATTTCTTCA ATATGGAAAA TTTCTATGCT CATATATTCA ATATCAAGTG TGCCCGCCAG
    TTCGAAGCTG CCTATCCAGC TCCAAGAGGG ATTCCCAAAG GAAAGTTGGT CAAGTATATG
    ATGGGATTCC CAATTATCAT TGGAGTCGTT ATCTTCATCT TTTCTCCTCT ATTGCTCTGG
    TCGCTACTCA ATCAAATCGG AACTATCAGT ATGCCAGAAA AAGTTACGTT GAGGATTTCG
    ATTGAAGGAT ATCCACCACT TTACGAGATG GAGGCACAAG GAAGTAATCA CGATAACGCT
    GAACTTGGAA TGATAAAACC TGATCAATTG GCATCTTTGA ATCAAGCATT GACGGATAGT
    TACACGACAC GAGACACTAA TTCTATTTTA AGATCAAGAA TGTCTGTATC ATATCTGAAA
    GGATACACAT ATGAGGATAT TCTGATTGTG CGATTCCGTC CAGAATCTGA AATTTATTGG
    CCGATTTCAC AGGATTCGAG AAATGCGATG ATCGATAAAT TGAGCCGAAA CACGTCAGTC
    AATTTTGAAG TATCTCTGGA ATTCACTCGG CCATATGATC CAAATGAAAA CGCTGCTTTA
    AAACATTCGA AATCGTGGCT TGTTCCAATC TCCCTGGATA TGACGATTCG AGCAAAAATT
    CAAAGTGCTC TCCGAGGAGA TCCAGGTCAT CCGATTCTCA TTCCACAGTC AATTCCTGCG
    TTTATTCAAG TTCCAAATCA AGGAGAATTG ACCCTTCCAA CATCAATCGG AAATACTATT
    ATCAATGACG GAAATCCACG GATTAATACG ACCGGGATGG AAAAATCAGA CGAAGCTCGG
    GCTTGGTTCG ACTCGTTAAC CTTGAATCTC GAACAAGGAA AATCGCAAAA CGAGAAAATG
    TGGATCGCCA CATCCGAACA TCCAGGAGAC CAAAATGCGA AACTCTGGAT AAAGACTGCA
    AATACGACGT ATTCGGGAAG GCCGTACCTT CAAGTTGTCG GATTTATTGA TAGAGCATTC
    CCATCATTTT TGGCGAAAGT TTTCAAAGGA GGAGTCATTG CAGTGTATCT TTCAGTTATC
    CTCGTCGTTG GAAGAGGTCT GGTTCGTGGA ATCTTCACCA CATCTCCAAG CACTGTCATG
    TTCACAGAAT TACCGAATGC GGATCATCTT CTCAAAATTT GTCTCGACAT CTATTTGGTT
    CGAGAAGCGA AGGACTTCAT GTTGGAGCAG GATCTCTTCG CCAAATTAAT ATTCCTGTTC
    CGATCACCAG CGACACTCAT CGAATGGACA CGAATGAGCA AAAAGAAGCA AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293677.1
    CDS1..3117
    Translation

    Target ORF information:

    RefSeq Version NM_001306748.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo_RRas_bdg domain-containing protein (pezo-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306748.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTCAAAT ACGATCCTGA AAATGATGAT CTTGTGGAAC CCGTCGACAG TTTTGTACCA 
    GAAGTTGATC CCAAGGCAAC TGCATATGAT CGACTTGATC CTGGACAGAT AATGTACGCT
    GCCACCGCTC ACGACCTCGA CCTCGCCAAA ACTGTACAGC AAGTCAAGAA AGGCGATACT
    ATCAAAGATC CTGATTCACG TGCTCTTATC GCTGTTTCTG AACCAGAAGC TCGTAAGCCA
    GGAGGCACTG AAGAAACGGA TGGTGATGAA GATGAGGATA ATAAGGATTC GAAAGTGGAA
    AGTACTGCTA AATTCATTCA AAAAATGATC GCTTCTGCCT TGGACTTGTG TAGTGTAACA
    CTAAACAAAC TGTGCAGAGA ACACCGATAT GTTGGATTTG TTTTGAGCAA GGAAAAACAA
    AAGTTGAAGT CAGGACACTC GGAATCTCTT TCAAATACAT CTAGAAAGCT GACGGATATT
    CGATCTGCAG TTGATCTACC AAGTCTTCAA CTTGTGCAAT CGGCGAATGA TGTTGAAAAG
    ATGGAGACAG CCGTCAGCGT GGATTGGCAA CAAAAATCAT CAGCAACGAG ACTATTGAAT
    GCAGTTGTGA ATTGTATTGG AGCACACACT GATATTCTCT GCTACTTCTT CGCAATTATG
    ACACAAGTGA TGACTGGTGG ACTTATTACT CTTCCACTTC CTCTGATGTC ACTATTCTGG
    GGAAATCTAT CGAATCCTCG TCCATCCAAG TTCTTCTGGG TCACAATGAT CACCTACACT
    GAGTGTGTCA TTGTTATCAA GTTTGTTTGC CAATTTGCAT TCATGCCTTA CAATTCTATC
    ACGTGGCGAA CAGAGCATCA AATGGACCCG ATGTCATTGG ATAAGTTATT CGGAGTATCA
    CAGAGAGATA GTTTTGCACT TTGGGACATT GTTTTGCTCT TCTCACTCTT CTTCCATCGG
    TATATGCTCA GAAAGTTAGG GTTATGGAAG GATGCCAATT TGACGGACAC GTTCACTTTG
    AAGGAAGAGC CTCGATCGGC GTCAGGATCG GACACAGGAT CACCCAAGAA AATTGCTCAA
    GAGCCAAAGG TTGTTGTAAC TCAGTCAGAT ACCCTTGAAG GTACAAGTGG AGGAGAGATA
    GTCATACCAT CTGATCCAAA TGCAGTTTCC AATATGGAAG AATTAGACTG CGAACCTCCG
    ATTCCAGAGA AGCAAAGTGG ACCAATTGGA AGATTCATTC ATCAATTATT CCATCCAAAA
    TTCCGTTACA TTCGTGATTT GTATCCAATA ATGTTTGGAA TCGATGTGAT ATGCTTCCTC
    ATCATGACAT TTGGATACTC GGCATTTGGT GAAGGAGGTT CTGGAAATGT TTTAGATGAT
    GTGAAGGCTT CTCGTATTCC GGTAACACTT GTTGTTATGC TCGTTGGAAT GACTCTGGCA
    ATTATCATTG ATCGAGCACT CTACCTCAGA AAATCAGTTG TCGGAAAGCT AATCTATCAA
    GTCTTAATGA TTGCTTTCCT TCACATTTGG GTATTCCTTG TTCTTCCAAA TATGACTCGT
    CGTTCTGCGA TATCGAATCA CGTTGCTCAA GCATTGTACG TCATCAAATC GTGCTATTTC
    CTTGTGTCTG CTTGGCAGAT CCGAAATGGT TATCCTGAAC TTTGTATTGG AAATCTATTA
    ACACATTCAT ACGGAATGAC CAATATGATT GCATTCAAAG TATTCATGAA TATTCCATTC
    CTCTTCGAGC TTCGTACAGC TATTGATTGG ACATGGACAG ATACTTCAAT GCCTCTTTTC
    GATTTCTTCA ATATGGAAAA TTTCTATGCT CATATATTCA ATATCAAGTG TGCCCGCCAG
    TTCGAAGCTG CCTATCCAGC TCCAAGAGGG ATTCCCAAAG GAAAGTTGGT CAAGTATATG
    ATGGGATTCC CAATTATCAT TGGAGTCGTT ATCTTCATCT TTTCTCCTCT ATTGCTCTGG
    TCGCTACTCA ATCAAATCGG AACTATCAGT ATGCCAGAAA AAGTTACGTT GAGGATTTCG
    ATTGAAGGAT ATCCACCACT TTACGAGATG GAGGCACAAG GAAGTAATCA CGATAACGCT
    GAACTTGGAA TGATAAAACC TGATCAATTG GCATCTTTGA ATCAAGCATT GACGGATAGT
    TACACGACAC GAGACACTAA TTCTATTTTA AGATCAAGAA TGTCTGTATC ATATCTGAAA
    GGATACACAT ATGAGGATAT TCTGATTGTG CGATTCCGTC CAGAATCTGA AATTTATTGG
    CCGATTTCAC AGGATTCGAG AAATGCGATG ATCGATAAAT TGAGCCGAAA CACGTCAGTC
    AATTTTGAAG TATCTCTGGA ATTCACTCGG CCATATGATC CAAATGAAAA CGCTGCTTTA
    AAACATTCGA AATCGTGGCT TGTTCCAATC TCCCTGGATA TGACGATTCG AGCAAAAATT
    CAAAGTGCTC TCCGAGGAGA TCCAGGTCAT CCGATTCTCA TTCCACAGTC AATTCCTGCG
    TTTATTCAAG TTCCAAATCA AGGAGAATTG ACCCTTCCAA CATCAATCGG AAATACTATT
    ATCAATGACG GAAATCCACG GATTAATACG ACCGGGATGG AAAAATCAGA CGAAGCTCGG
    GCTTGGTTCG ACTCGTTAAC CTTGAATCTC GAACAAGGAA AATCGCAAAA CGAGAAAATG
    TGGATCGCCA CATCCGAACA TCCAGGAGAC CAAAATGCGA AACTCTGGAT AAAGACTGCA
    AATACGACGT ATTCGGGAAG GCCGTACCTT CAAGTTGTCG GATTTATTGA TAGAGCATTC
    CCATCATTTT TGGCGAAAGT TTTCAAAGGA GGAGTCATTG CAGTGTATCT TTCAGTTATC
    CTCGTCGTTG GAAGAGGTCT GGTTCGTGGA ATCTTCACCA CATCTCCAAG CACTGTCATG
    TTCACAGAAT TACCGAATGC GGATCATCTT CTCAAAATTT GTCTCGACAT CTATTTGGTT
    CGAGAAGCGA AGGACTTCAT GTTGGAGCAG GATCTCTTCG CCAAATTAAT ATTCCTGTTC
    CGATCACCAG CGACACTCAT CGAATGGACA CGAATGAGCA AAAAGAAGCA AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa356221
    Clone ID Related Accession (Same CDS sequence) NM_001307053.1 , NM_001372904.1
    Accession Version NM_001307053.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5061bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTCCAAC TCAAGTTTTT CCATGGACCA TGGAGTCGAG CAACGTCACC AAGAAGAGCA 
    GAAAATGATC CACCTACAAG TACGACGGAG GCTGCGGCGG TAGCAAGCAC ATCTGGAACA
    CAAGGAAGAG CACATGCTGC AGGCGATACC CTTGTCAAGA AGCTGCACAA ATTGGCAAAT
    CAGACAATCG AACTGTTATG GAGGTTTTTC GAAGTTCACA TTTCGAAAAT TGTCTTTGTC
    ATCATTGCGA TTTTTATTGC CAACAATATC AATGCTCTAT ACATTCCACT TGTTATTCTC
    CTCTCATTGG CTATATGTCT ACCATCAGCT GCAGATGGGA TATTCTCTTT ATTTATGTGT
    GCCTACTTAT TCCTCGTGGC ACTGAGCAAG ATGATCTACC AACTGGATAT TGTTCCCGAG
    TTATCACAAA TCGACCGAGG AGTTGGAGCA GATAACTGTT CACATGGAAA TATTTCAATG
    CCTGAATGGT TTGGATTGAA GAAGGAAGTT GAAGGAACTG AACCAATTTA TATGCTCTTT
    GGAGTCATTG TTTCGATTAT TGCATTGGCT TTTCAATCAA TTGTTATATA TCGACAAAGA
    CATTATCGAG CTTCACTGGG ACTTCCTGAA TCGATGCGAG CTAAAGTGTT CCCGGACTTC
    CATCATTCTC ATTTTGATAG ATCTCTCAAA AATGCCATCC AATTCTTGAT AGACTATGGT
    TTCTATAAGT TTGGACTTGA AATCACGATG ATCGCCATTG GAATTGACAT TTTCAATCGA
    ATGGATGCAT TGGCAGCGAT TCAATGTTTC TGGCTTGTCC TGTTTGCGCT CAATAAAAGA
    GTCTTTGTTC GAAGAATATG GGTATTCTAT GTGATCTATA TGGCGATTCT GTATCCACTT
    CAGTTCTTTT CATATGTTGG ATTACCACCT GACAGTTGTA TTGAGTATCC ATGGTCGTAT
    TGGATTCCAT CGTATAGTGA TGATGCGAGA TTCAATCTGT CGTATCTTCT GAACTTGTCA
    ATATACGGTG TAAACTGGCC ATCAGCATAT CTCATTGGCG ATTTCTTCGT ACTCCTCTTG
    GCATCATGCC AATTAGCAGT TTTCCGGCGA GAAGGAGAAG ATAATGATTC AATTTACAAT
    GATGGAAACT TTGTGATTAA GCCAGAAAAT CCACAATATG ACTTTATTGA CACCAAAAAG
    AGTTACGTTG ATTATTTCAA ATCATTCGTT TTCCATTATG GACACTGGAT TACATTGATG
    TCTACACTAG CAGCAGGAAT TGCTGGAACG TCACTTTTTG CTTTGGGATA CATTATATTC
    ACTCTGACAA TGCTGTGGTC TGGTAACAAT TTGTATGTGA TGAACTCAAC TCTACGATCA
    TTTGAACATA CTTTGAAACG ATGGAATGCA CTTTTGGGAT ACACATTGTT CACGATTACA
    ATGAAAGTTT GCCTTCAAAT ATTTGGATGT GTATTCCTGT CGTGGTTCGA TCAATCTGGT
    GGATGGGGAA AGACTCTGTG CATCGTTCGT CAACTCTTCA GTATCACATG TGTTAATAAC
    GAATGTCATG TGCTCAAAGA GCTGGAGGAT TTCTCGAAGG CGTGTGCTGT TGAGACGAAA
    GAAGGAAATA TTGGATTTGA TGTGATTGCG CTATCCTTCC TCGTCTTCCA AATCCGAATT
    TTCCATTCTT GGTATTTCCA ACACTGCATG GTTGAATATC GAAGTGAGGT TATTCTGGCC
    AATAGAGGTG CCGTGCTGAA GAATCAACTG ATTGAAAAGG AAATGAAGGA ACAAAACGAG
    CAGCAAAAAG CCAAGTTCAA TGATATCAGA AGAAGAACTG AAGCTATTCG TGAAAGATAT
    CAAAAACAAA TCGAACGAGG AGCTGCTGAA CGTGACTTCG AGCCGGTTAC TTATGGGCAC
    GCCAAACGAG CGGGAGACTA CTACATGTTC AAATACGATC CTGAAAATGA TGATCTTGTG
    GAACCCGTCG ACAGTTTTGT ACCAGAAGTT GATCCCAAGG CAACTGCATA TGATCGACTT
    GATCCTGGAC AGATAATGTA CGCTGCCACC GCTCACGACC TCGACCTCGC CAAAACTGTA
    CAGCAAGTCA AGAAAGGCGA TACTATCAAA GATCCTGATT CACGTGCTCT TATCGCTGTT
    TCTGAACCAG AAGCTCGTAA GCCAGGAGGC ACTGAAGAAA CGGATGGTGA TGAAGATGAG
    GATAATAAGG ATTCGAAAGT GGAAAGTACT GCTAAATTCA TTCAAAAAAT GATCGCTTCT
    GCCTTGGACT TGTGTAGTGT AACACTAAAC AAACTGTGCA GAGAACACCG ATATGTTGGA
    TTTGTTTTGA GCAAGGAAAA ACAAAAGTTG AAGTCAGGAC ACTCGGAATC TCTTTCAAAT
    ACATCTAGAA AGCTGACGGA TATTCGATCT GCAGTTGATC TACCAAGTCT TCAACTTGTG
    CAATCGGCGA ATGATGTTGA AAAGATGGAG ACAGCCGTCA GCGTGGATTG GCAACAAAAA
    TCATCAGCAA CGAGACTATT GAATGCAGTT GTGAATTGTA TTGGAGCACA CACTGATATT
    CTCTGCTACT TCTTCGCAAT TATGACACAA GTGATGACTG GTGGACTTAT TACTCTTCCA
    CTTCCTCTGA TGTCACTATT CTGGGGAAAT CTATCGAATC CTCGTCCATC CAAGTTCTTC
    TGGGTCACAA TGATCACCTA CACTGAGTGT GTCATTGTTA TCAAGTTTGT TTGCCAATTT
    GCATTCATGC CTTACAATTC TATCACGTGG CGAACAGAGC ATCAAATGGA CCCGATGTCA
    TTGGATAAGT TATTCGGAGT ATCACAGAGA GATAGTTTTG CACTTTGGGA CATTGTTTTG
    CTCTTCTCAC TCTTCTTCCA TCGGTATATG CTCAGAAAGT TAGGGTTATG GAAGGATGCC
    AATTTGACGG ACACGTTCAC TTTGAAGGAA GAGCCTCGAT CGGCGTCAGG ATCGGACACA
    GGATCACCCA AGAAAATTGC TCAAGAGCCA AAGGTTGTTG TAACTCAGTC AGATACCCTT
    GAAGGTACAA GTGGAGGAGA GATAGTCATA CCATCTGATC CAAATGCAGT TTCCAATATG
    GAAGAATTAG ACTGCGAACC TCCGATTCCA GAGAAGCAAA GTGGACCAAT TGGAAGATTC
    ATTCATCAAT TATTCCATCC AAAATTCCGT TACATTCGTG ATTTGTATCC AATAATGTTT
    GGAATCGATG TGATATGCTT CCTCATCATG ACATTTGGAT ACTCGGCATT TGGTGAAGGA
    GGTTCTGGAA ATGTTTTAGA TGATGTGAAG GCTTCTCGTA TTCCGGTAAC ACTTGTTGTT
    ATGCTCGTTG GAATGACTCT GGCAATTATC ATTGATCGAG CACTCTACCT CAGAAAATCA
    GTTGTCGGAA AGCTAATCTA TCAAGTCTTA ATGATTGCTT TCCTTCACAT TTGGGTATTC
    CTTGTTCTTC CAAATATGAC TCGTCGTTCT GCGATATCGA ATCACGTTGC TCAAGCATTG
    TACGTCATCA AATCGTGCTA TTTCCTTGTG TCTGCTTGGC AGATCCGAAA TGGTTATCCT
    GAACTTTGTA TTGGAAATCT ATTAACACAT TCATACGGAA TGACCAATAT GATTGCATTC
    AAAGTATTCA TGAATATTCC ATTCCTCTTC GAGCTTCGTA CAGCTATTGA TTGGACATGG
    ACAGATACTT CAATGCCTCT TTTCGATTTC TTCAATATGG AAAATTTCTA TGCTCATATA
    TTCAATATCA AGTGTGCCCG CCAGTTCGAA GCTGCCTATC CAGCTCCAAG AGGGATTCCC
    AAAGGAAAGT TGGTCAAGTA TATGATGGGA TTCCCAATTA TCATTGGAGT CGTTATCTTC
    ATCTTTTCTC CTCTATTGCT CTGGTCGCTA CTCAATCAAA TCGGAACTAT CAGTATGCCA
    GAAAAAGTTA CGTTGAGGAT TTCGATTGAA GGATATCCAC CACTTTACGA GATGGAGGCA
    CAAGGAAGTA ATCACGATAA CGCTGAACTT GGAATGATAA AACCTGATCA ATTGGCATCT
    TTGAATCAAG CATTGACGGA TAGTTACACG ACACGAGACA CTAATTCTAT TTTAAGATCA
    AGAATGTCTG TATCATATCT GAAAGGATAC ACATATGAGG ATATTCTGAT TGTGCGATTC
    CGTCCAGAAT CTGAAATTTA TTGGCCGATT TCACAGGATT CGAGAAATGC GATGATCGAT
    AAATTGAGCC GAAACACGTC AGTCAATTTT GAAGTATCTC TGGAATTCAC TCGGCCATAT
    GATCCAAATG AAAACGCTGC TTTAAAACAT TCGAAATCGT GGCTTGTTCC AATCTCCCTG
    GATATGACGA TTCGAGCAAA AATTCAAAGT GCTCTCCGAG GAGATCCAGG TCATCCGATT
    CTCATTCCAC AGTCAATTCC TGCGTTTATT CAAGTTCCAA ATCAAGGAGA ATTGACCCTT
    CCAACATCAA TCGGAAATAC TATTATCAAT GACGGAAATC CACGGATTAA TACGACCGGG
    ATGGAAAAAT CAGACGAAGC TCGGGCTTGG TTCGACTCGT TAACCTTGAA TCTCGAACAA
    GGAAAATCGC AAAACGAGAA AATGTGGATC GCCACATCCG AACATCCAGG AGACCAAAAT
    GCGAAACTCT GGATAAAGAC TGCAAATACG ACGTATTCGG GAAGGCCGTA CCTTCAAGTT
    GTCGGATTTA TTGATAGAGC ATTCCCATCA TTTTTGGCGA AAGTTTTCAA AGGAGGAGTC
    ATTGCAGTGT ATCTTTCAGT TATCCTCGTC GTTGGAAGAG GTCTGGTTCG TGGAATCTTC
    ACCACATCTC CAAGCACTGT CATGTTCACA GAATTACCGA ATGCGGATCA TCTTCTCAAA
    ATTTGTCTCG ACATCTATTT GGTTCGAGAA GCGAAGGACT TCATGTTGGA GCAGGATCTC
    TTCGCCAAAT TAATATTCCT GTTCCGATCA CCAGCGACAC TCATCGAATG GACACGAATG
    AGCAAAAAGA AGCAAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293982.1
    CDS1..5061
    Translation

    Target ORF information:

    RefSeq Version NM_001307053.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307053.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTCCAAC TCAAGTTTTT CCATGGACCA TGGAGTCGAG CAACGTCACC AAGAAGAGCA 
    GAAAATGATC CACCTACAAG TACGACGGAG GCTGCGGCGG TAGCAAGCAC ATCTGGAACA
    CAAGGAAGAG CACATGCTGC AGGCGATACC CTTGTCAAGA AGCTGCACAA ATTGGCAAAT
    CAGACAATCG AACTGTTATG GAGGTTTTTC GAAGTTCACA TTTCGAAAAT TGTCTTTGTC
    ATCATTGCGA TTTTTATTGC CAACAATATC AATGCTCTAT ACATTCCACT TGTTATTCTC
    CTCTCATTGG CTATATGTCT ACCATCAGCT GCAGATGGGA TATTCTCTTT ATTTATGTGT
    GCCTACTTAT TCCTCGTGGC ACTGAGCAAG ATGATCTACC AACTGGATAT TGTTCCCGAG
    TTATCACAAA TCGACCGAGG AGTTGGAGCA GATAACTGTT CACATGGAAA TATTTCAATG
    CCTGAATGGT TTGGATTGAA GAAGGAAGTT GAAGGAACTG AACCAATTTA TATGCTCTTT
    GGAGTCATTG TTTCGATTAT TGCATTGGCT TTTCAATCAA TTGTTATATA TCGACAAAGA
    CATTATCGAG CTTCACTGGG ACTTCCTGAA TCGATGCGAG CTAAAGTGTT CCCGGACTTC
    CATCATTCTC ATTTTGATAG ATCTCTCAAA AATGCCATCC AATTCTTGAT AGACTATGGT
    TTCTATAAGT TTGGACTTGA AATCACGATG ATCGCCATTG GAATTGACAT TTTCAATCGA
    ATGGATGCAT TGGCAGCGAT TCAATGTTTC TGGCTTGTCC TGTTTGCGCT CAATAAAAGA
    GTCTTTGTTC GAAGAATATG GGTATTCTAT GTGATCTATA TGGCGATTCT GTATCCACTT
    CAGTTCTTTT CATATGTTGG ATTACCACCT GACAGTTGTA TTGAGTATCC ATGGTCGTAT
    TGGATTCCAT CGTATAGTGA TGATGCGAGA TTCAATCTGT CGTATCTTCT GAACTTGTCA
    ATATACGGTG TAAACTGGCC ATCAGCATAT CTCATTGGCG ATTTCTTCGT ACTCCTCTTG
    GCATCATGCC AATTAGCAGT TTTCCGGCGA GAAGGAGAAG ATAATGATTC AATTTACAAT
    GATGGAAACT TTGTGATTAA GCCAGAAAAT CCACAATATG ACTTTATTGA CACCAAAAAG
    AGTTACGTTG ATTATTTCAA ATCATTCGTT TTCCATTATG GACACTGGAT TACATTGATG
    TCTACACTAG CAGCAGGAAT TGCTGGAACG TCACTTTTTG CTTTGGGATA CATTATATTC
    ACTCTGACAA TGCTGTGGTC TGGTAACAAT TTGTATGTGA TGAACTCAAC TCTACGATCA
    TTTGAACATA CTTTGAAACG ATGGAATGCA CTTTTGGGAT ACACATTGTT CACGATTACA
    ATGAAAGTTT GCCTTCAAAT ATTTGGATGT GTATTCCTGT CGTGGTTCGA TCAATCTGGT
    GGATGGGGAA AGACTCTGTG CATCGTTCGT CAACTCTTCA GTATCACATG TGTTAATAAC
    GAATGTCATG TGCTCAAAGA GCTGGAGGAT TTCTCGAAGG CGTGTGCTGT TGAGACGAAA
    GAAGGAAATA TTGGATTTGA TGTGATTGCG CTATCCTTCC TCGTCTTCCA AATCCGAATT
    TTCCATTCTT GGTATTTCCA ACACTGCATG GTTGAATATC GAAGTGAGGT TATTCTGGCC
    AATAGAGGTG CCGTGCTGAA GAATCAACTG ATTGAAAAGG AAATGAAGGA ACAAAACGAG
    CAGCAAAAAG CCAAGTTCAA TGATATCAGA AGAAGAACTG AAGCTATTCG TGAAAGATAT
    CAAAAACAAA TCGAACGAGG AGCTGCTGAA CGTGACTTCG AGCCGGTTAC TTATGGGCAC
    GCCAAACGAG CGGGAGACTA CTACATGTTC AAATACGATC CTGAAAATGA TGATCTTGTG
    GAACCCGTCG ACAGTTTTGT ACCAGAAGTT GATCCCAAGG CAACTGCATA TGATCGACTT
    GATCCTGGAC AGATAATGTA CGCTGCCACC GCTCACGACC TCGACCTCGC CAAAACTGTA
    CAGCAAGTCA AGAAAGGCGA TACTATCAAA GATCCTGATT CACGTGCTCT TATCGCTGTT
    TCTGAACCAG AAGCTCGTAA GCCAGGAGGC ACTGAAGAAA CGGATGGTGA TGAAGATGAG
    GATAATAAGG ATTCGAAAGT GGAAAGTACT GCTAAATTCA TTCAAAAAAT GATCGCTTCT
    GCCTTGGACT TGTGTAGTGT AACACTAAAC AAACTGTGCA GAGAACACCG ATATGTTGGA
    TTTGTTTTGA GCAAGGAAAA ACAAAAGTTG AAGTCAGGAC ACTCGGAATC TCTTTCAAAT
    ACATCTAGAA AGCTGACGGA TATTCGATCT GCAGTTGATC TACCAAGTCT TCAACTTGTG
    CAATCGGCGA ATGATGTTGA AAAGATGGAG ACAGCCGTCA GCGTGGATTG GCAACAAAAA
    TCATCAGCAA CGAGACTATT GAATGCAGTT GTGAATTGTA TTGGAGCACA CACTGATATT
    CTCTGCTACT TCTTCGCAAT TATGACACAA GTGATGACTG GTGGACTTAT TACTCTTCCA
    CTTCCTCTGA TGTCACTATT CTGGGGAAAT CTATCGAATC CTCGTCCATC CAAGTTCTTC
    TGGGTCACAA TGATCACCTA CACTGAGTGT GTCATTGTTA TCAAGTTTGT TTGCCAATTT
    GCATTCATGC CTTACAATTC TATCACGTGG CGAACAGAGC ATCAAATGGA CCCGATGTCA
    TTGGATAAGT TATTCGGAGT ATCACAGAGA GATAGTTTTG CACTTTGGGA CATTGTTTTG
    CTCTTCTCAC TCTTCTTCCA TCGGTATATG CTCAGAAAGT TAGGGTTATG GAAGGATGCC
    AATTTGACGG ACACGTTCAC TTTGAAGGAA GAGCCTCGAT CGGCGTCAGG ATCGGACACA
    GGATCACCCA AGAAAATTGC TCAAGAGCCA AAGGTTGTTG TAACTCAGTC AGATACCCTT
    GAAGGTACAA GTGGAGGAGA GATAGTCATA CCATCTGATC CAAATGCAGT TTCCAATATG
    GAAGAATTAG ACTGCGAACC TCCGATTCCA GAGAAGCAAA GTGGACCAAT TGGAAGATTC
    ATTCATCAAT TATTCCATCC AAAATTCCGT TACATTCGTG ATTTGTATCC AATAATGTTT
    GGAATCGATG TGATATGCTT CCTCATCATG ACATTTGGAT ACTCGGCATT TGGTGAAGGA
    GGTTCTGGAA ATGTTTTAGA TGATGTGAAG GCTTCTCGTA TTCCGGTAAC ACTTGTTGTT
    ATGCTCGTTG GAATGACTCT GGCAATTATC ATTGATCGAG CACTCTACCT CAGAAAATCA
    GTTGTCGGAA AGCTAATCTA TCAAGTCTTA ATGATTGCTT TCCTTCACAT TTGGGTATTC
    CTTGTTCTTC CAAATATGAC TCGTCGTTCT GCGATATCGA ATCACGTTGC TCAAGCATTG
    TACGTCATCA AATCGTGCTA TTTCCTTGTG TCTGCTTGGC AGATCCGAAA TGGTTATCCT
    GAACTTTGTA TTGGAAATCT ATTAACACAT TCATACGGAA TGACCAATAT GATTGCATTC
    AAAGTATTCA TGAATATTCC ATTCCTCTTC GAGCTTCGTA CAGCTATTGA TTGGACATGG
    ACAGATACTT CAATGCCTCT TTTCGATTTC TTCAATATGG AAAATTTCTA TGCTCATATA
    TTCAATATCA AGTGTGCCCG CCAGTTCGAA GCTGCCTATC CAGCTCCAAG AGGGATTCCC
    AAAGGAAAGT TGGTCAAGTA TATGATGGGA TTCCCAATTA TCATTGGAGT CGTTATCTTC
    ATCTTTTCTC CTCTATTGCT CTGGTCGCTA CTCAATCAAA TCGGAACTAT CAGTATGCCA
    GAAAAAGTTA CGTTGAGGAT TTCGATTGAA GGATATCCAC CACTTTACGA GATGGAGGCA
    CAAGGAAGTA ATCACGATAA CGCTGAACTT GGAATGATAA AACCTGATCA ATTGGCATCT
    TTGAATCAAG CATTGACGGA TAGTTACACG ACACGAGACA CTAATTCTAT TTTAAGATCA
    AGAATGTCTG TATCATATCT GAAAGGATAC ACATATGAGG ATATTCTGAT TGTGCGATTC
    CGTCCAGAAT CTGAAATTTA TTGGCCGATT TCACAGGATT CGAGAAATGC GATGATCGAT
    AAATTGAGCC GAAACACGTC AGTCAATTTT GAAGTATCTC TGGAATTCAC TCGGCCATAT
    GATCCAAATG AAAACGCTGC TTTAAAACAT TCGAAATCGT GGCTTGTTCC AATCTCCCTG
    GATATGACGA TTCGAGCAAA AATTCAAAGT GCTCTCCGAG GAGATCCAGG TCATCCGATT
    CTCATTCCAC AGTCAATTCC TGCGTTTATT CAAGTTCCAA ATCAAGGAGA ATTGACCCTT
    CCAACATCAA TCGGAAATAC TATTATCAAT GACGGAAATC CACGGATTAA TACGACCGGG
    ATGGAAAAAT CAGACGAAGC TCGGGCTTGG TTCGACTCGT TAACCTTGAA TCTCGAACAA
    GGAAAATCGC AAAACGAGAA AATGTGGATC GCCACATCCG AACATCCAGG AGACCAAAAT
    GCGAAACTCT GGATAAAGAC TGCAAATACG ACGTATTCGG GAAGGCCGTA CCTTCAAGTT
    GTCGGATTTA TTGATAGAGC ATTCCCATCA TTTTTGGCGA AAGTTTTCAA AGGAGGAGTC
    ATTGCAGTGT ATCTTTCAGT TATCCTCGTC GTTGGAAGAG GTCTGGTTCG TGGAATCTTC
    ACCACATCTC CAAGCACTGT CATGTTCACA GAATTACCGA ATGCGGATCA TCTTCTCAAA
    ATTTGTCTCG ACATCTATTT GGTTCGAGAA GCGAAGGACT TCATGTTGGA GCAGGATCTC
    TTCGCCAAAT TAATATTCCT GTTCCGATCA CCAGCGACAC TCATCGAATG GACACGAATG
    AGCAAAAAGA AGCAAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa356221
    Clone ID Related Accession (Same CDS sequence) NM_001307053.1 , NM_001372904.1
    Accession Version NM_001372904.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5061bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTCCAAC TCAAGTTTTT CCATGGACCA TGGAGTCGAG CAACGTCACC AAGAAGAGCA 
    GAAAATGATC CACCTACAAG TACGACGGAG GCTGCGGCGG TAGCAAGCAC ATCTGGAACA
    CAAGGAAGAG CACATGCTGC AGGCGATACC CTTGTCAAGA AGCTGCACAA ATTGGCAAAT
    CAGACAATCG AACTGTTATG GAGGTTTTTC GAAGTTCACA TTTCGAAAAT TGTCTTTGTC
    ATCATTGCGA TTTTTATTGC CAACAATATC AATGCTCTAT ACATTCCACT TGTTATTCTC
    CTCTCATTGG CTATATGTCT ACCATCAGCT GCAGATGGGA TATTCTCTTT ATTTATGTGT
    GCCTACTTAT TCCTCGTGGC ACTGAGCAAG ATGATCTACC AACTGGATAT TGTTCCCGAG
    TTATCACAAA TCGACCGAGG AGTTGGAGCA GATAACTGTT CACATGGAAA TATTTCAATG
    CCTGAATGGT TTGGATTGAA GAAGGAAGTT GAAGGAACTG AACCAATTTA TATGCTCTTT
    GGAGTCATTG TTTCGATTAT TGCATTGGCT TTTCAATCAA TTGTTATATA TCGACAAAGA
    CATTATCGAG CTTCACTGGG ACTTCCTGAA TCGATGCGAG CTAAAGTGTT CCCGGACTTC
    CATCATTCTC ATTTTGATAG ATCTCTCAAA AATGCCATCC AATTCTTGAT AGACTATGGT
    TTCTATAAGT TTGGACTTGA AATCACGATG ATCGCCATTG GAATTGACAT TTTCAATCGA
    ATGGATGCAT TGGCAGCGAT TCAATGTTTC TGGCTTGTCC TGTTTGCGCT CAATAAAAGA
    GTCTTTGTTC GAAGAATATG GGTATTCTAT GTGATCTATA TGGCGATTCT GTATCCACTT
    CAGTTCTTTT CATATGTTGG ATTACCACCT GACAGTTGTA TTGAGTATCC ATGGTCGTAT
    TGGATTCCAT CGTATAGTGA TGATGCGAGA TTCAATCTGT CGTATCTTCT GAACTTGTCA
    ATATACGGTG TAAACTGGCC ATCAGCATAT CTCATTGGCG ATTTCTTCGT ACTCCTCTTG
    GCATCATGCC AATTAGCAGT TTTCCGGCGA GAAGGAGAAG ATAATGATTC AATTTACAAT
    GATGGAAACT TTGTGATTAA GCCAGAAAAT CCACAATATG ACTTTATTGA CACCAAAAAG
    AGTTACGTTG ATTATTTCAA ATCATTCGTT TTCCATTATG GACACTGGAT TACATTGATG
    TCTACACTAG CAGCAGGAAT TGCTGGAACG TCACTTTTTG CTTTGGGATA CATTATATTC
    ACTCTGACAA TGCTGTGGTC TGGTAACAAT TTGTATGTGA TGAACTCAAC TCTACGATCA
    TTTGAACATA CTTTGAAACG ATGGAATGCA CTTTTGGGAT ACACATTGTT CACGATTACA
    ATGAAAGTTT GCCTTCAAAT ATTTGGATGT GTATTCCTGT CGTGGTTCGA TCAATCTGGT
    GGATGGGGAA AGACTCTGTG CATCGTTCGT CAACTCTTCA GTATCACATG TGTTAATAAC
    GAATGTCATG TGCTCAAAGA GCTGGAGGAT TTCTCGAAGG CGTGTGCTGT TGAGACGAAA
    GAAGGAAATA TTGGATTTGA TGTGATTGCG CTATCCTTCC TCGTCTTCCA AATCCGAATT
    TTCCATTCTT GGTATTTCCA ACACTGCATG GTTGAATATC GAAGTGAGGT TATTCTGGCC
    AATAGAGGTG CCGTGCTGAA GAATCAACTG ATTGAAAAGG AAATGAAGGA ACAAAACGAG
    CAGCAAAAAG CCAAGTTCAA TGATATCAGA AGAAGAACTG AAGCTATTCG TGAAAGATAT
    CAAAAACAAA TCGAACGAGG AGCTGCTGAA CGTGACTTCG AGCCGGTTAC TTATGGGCAC
    GCCAAACGAG CGGGAGACTA CTACATGTTC AAATACGATC CTGAAAATGA TGATCTTGTG
    GAACCCGTCG ACAGTTTTGT ACCAGAAGTT GATCCCAAGG CAACTGCATA TGATCGACTT
    GATCCTGGAC AGATAATGTA CGCTGCCACC GCTCACGACC TCGACCTCGC CAAAACTGTA
    CAGCAAGTCA AGAAAGGCGA TACTATCAAA GATCCTGATT CACGTGCTCT TATCGCTGTT
    TCTGAACCAG AAGCTCGTAA GCCAGGAGGC ACTGAAGAAA CGGATGGTGA TGAAGATGAG
    GATAATAAGG ATTCGAAAGT GGAAAGTACT GCTAAATTCA TTCAAAAAAT GATCGCTTCT
    GCCTTGGACT TGTGTAGTGT AACACTAAAC AAACTGTGCA GAGAACACCG ATATGTTGGA
    TTTGTTTTGA GCAAGGAAAA ACAAAAGTTG AAGTCAGGAC ACTCGGAATC TCTTTCAAAT
    ACATCTAGAA AGCTGACGGA TATTCGATCT GCAGTTGATC TACCAAGTCT TCAACTTGTG
    CAATCGGCGA ATGATGTTGA AAAGATGGAG ACAGCCGTCA GCGTGGATTG GCAACAAAAA
    TCATCAGCAA CGAGACTATT GAATGCAGTT GTGAATTGTA TTGGAGCACA CACTGATATT
    CTCTGCTACT TCTTCGCAAT TATGACACAA GTGATGACTG GTGGACTTAT TACTCTTCCA
    CTTCCTCTGA TGTCACTATT CTGGGGAAAT CTATCGAATC CTCGTCCATC CAAGTTCTTC
    TGGGTCACAA TGATCACCTA CACTGAGTGT GTCATTGTTA TCAAGTTTGT TTGCCAATTT
    GCATTCATGC CTTACAATTC TATCACGTGG CGAACAGAGC ATCAAATGGA CCCGATGTCA
    TTGGATAAGT TATTCGGAGT ATCACAGAGA GATAGTTTTG CACTTTGGGA CATTGTTTTG
    CTCTTCTCAC TCTTCTTCCA TCGGTATATG CTCAGAAAGT TAGGGTTATG GAAGGATGCC
    AATTTGACGG ACACGTTCAC TTTGAAGGAA GAGCCTCGAT CGGCGTCAGG ATCGGACACA
    GGATCACCCA AGAAAATTGC TCAAGAGCCA AAGGTTGTTG TAACTCAGTC AGATACCCTT
    GAAGGTACAA GTGGAGGAGA GATAGTCATA CCATCTGATC CAAATGCAGT TTCCAATATG
    GAAGAATTAG ACTGCGAACC TCCGATTCCA GAGAAGCAAA GTGGACCAAT TGGAAGATTC
    ATTCATCAAT TATTCCATCC AAAATTCCGT TACATTCGTG ATTTGTATCC AATAATGTTT
    GGAATCGATG TGATATGCTT CCTCATCATG ACATTTGGAT ACTCGGCATT TGGTGAAGGA
    GGTTCTGGAA ATGTTTTAGA TGATGTGAAG GCTTCTCGTA TTCCGGTAAC ACTTGTTGTT
    ATGCTCGTTG GAATGACTCT GGCAATTATC ATTGATCGAG CACTCTACCT CAGAAAATCA
    GTTGTCGGAA AGCTAATCTA TCAAGTCTTA ATGATTGCTT TCCTTCACAT TTGGGTATTC
    CTTGTTCTTC CAAATATGAC TCGTCGTTCT GCGATATCGA ATCACGTTGC TCAAGCATTG
    TACGTCATCA AATCGTGCTA TTTCCTTGTG TCTGCTTGGC AGATCCGAAA TGGTTATCCT
    GAACTTTGTA TTGGAAATCT ATTAACACAT TCATACGGAA TGACCAATAT GATTGCATTC
    AAAGTATTCA TGAATATTCC ATTCCTCTTC GAGCTTCGTA CAGCTATTGA TTGGACATGG
    ACAGATACTT CAATGCCTCT TTTCGATTTC TTCAATATGG AAAATTTCTA TGCTCATATA
    TTCAATATCA AGTGTGCCCG CCAGTTCGAA GCTGCCTATC CAGCTCCAAG AGGGATTCCC
    AAAGGAAAGT TGGTCAAGTA TATGATGGGA TTCCCAATTA TCATTGGAGT CGTTATCTTC
    ATCTTTTCTC CTCTATTGCT CTGGTCGCTA CTCAATCAAA TCGGAACTAT CAGTATGCCA
    GAAAAAGTTA CGTTGAGGAT TTCGATTGAA GGATATCCAC CACTTTACGA GATGGAGGCA
    CAAGGAAGTA ATCACGATAA CGCTGAACTT GGAATGATAA AACCTGATCA ATTGGCATCT
    TTGAATCAAG CATTGACGGA TAGTTACACG ACACGAGACA CTAATTCTAT TTTAAGATCA
    AGAATGTCTG TATCATATCT GAAAGGATAC ACATATGAGG ATATTCTGAT TGTGCGATTC
    CGTCCAGAAT CTGAAATTTA TTGGCCGATT TCACAGGATT CGAGAAATGC GATGATCGAT
    AAATTGAGCC GAAACACGTC AGTCAATTTT GAAGTATCTC TGGAATTCAC TCGGCCATAT
    GATCCAAATG AAAACGCTGC TTTAAAACAT TCGAAATCGT GGCTTGTTCC AATCTCCCTG
    GATATGACGA TTCGAGCAAA AATTCAAAGT GCTCTCCGAG GAGATCCAGG TCATCCGATT
    CTCATTCCAC AGTCAATTCC TGCGTTTATT CAAGTTCCAA ATCAAGGAGA ATTGACCCTT
    CCAACATCAA TCGGAAATAC TATTATCAAT GACGGAAATC CACGGATTAA TACGACCGGG
    ATGGAAAAAT CAGACGAAGC TCGGGCTTGG TTCGACTCGT TAACCTTGAA TCTCGAACAA
    GGAAAATCGC AAAACGAGAA AATGTGGATC GCCACATCCG AACATCCAGG AGACCAAAAT
    GCGAAACTCT GGATAAAGAC TGCAAATACG ACGTATTCGG GAAGGCCGTA CCTTCAAGTT
    GTCGGATTTA TTGATAGAGC ATTCCCATCA TTTTTGGCGA AAGTTTTCAA AGGAGGAGTC
    ATTGCAGTGT ATCTTTCAGT TATCCTCGTC GTTGGAAGAG GTCTGGTTCG TGGAATCTTC
    ACCACATCTC CAAGCACTGT CATGTTCACA GAATTACCGA ATGCGGATCA TCTTCTCAAA
    ATTTGTCTCG ACATCTATTT GGTTCGAGAA GCGAAGGACT TCATGTTGGA GCAGGATCTC
    TTCGCCAAAT TAATATTCCT GTTCCGATCA CCAGCGACAC TCATCGAATG GACACGAATG
    AGCAAAAAGA AGCAAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001360367.1
    CDS2310..7370
    Translation

    Target ORF information:

    RefSeq Version NM_001372904.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001372904.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTCCAAC TCAAGTTTTT CCATGGACCA TGGAGTCGAG CAACGTCACC AAGAAGAGCA 
    GAAAATGATC CACCTACAAG TACGACGGAG GCTGCGGCGG TAGCAAGCAC ATCTGGAACA
    CAAGGAAGAG CACATGCTGC AGGCGATACC CTTGTCAAGA AGCTGCACAA ATTGGCAAAT
    CAGACAATCG AACTGTTATG GAGGTTTTTC GAAGTTCACA TTTCGAAAAT TGTCTTTGTC
    ATCATTGCGA TTTTTATTGC CAACAATATC AATGCTCTAT ACATTCCACT TGTTATTCTC
    CTCTCATTGG CTATATGTCT ACCATCAGCT GCAGATGGGA TATTCTCTTT ATTTATGTGT
    GCCTACTTAT TCCTCGTGGC ACTGAGCAAG ATGATCTACC AACTGGATAT TGTTCCCGAG
    TTATCACAAA TCGACCGAGG AGTTGGAGCA GATAACTGTT CACATGGAAA TATTTCAATG
    CCTGAATGGT TTGGATTGAA GAAGGAAGTT GAAGGAACTG AACCAATTTA TATGCTCTTT
    GGAGTCATTG TTTCGATTAT TGCATTGGCT TTTCAATCAA TTGTTATATA TCGACAAAGA
    CATTATCGAG CTTCACTGGG ACTTCCTGAA TCGATGCGAG CTAAAGTGTT CCCGGACTTC
    CATCATTCTC ATTTTGATAG ATCTCTCAAA AATGCCATCC AATTCTTGAT AGACTATGGT
    TTCTATAAGT TTGGACTTGA AATCACGATG ATCGCCATTG GAATTGACAT TTTCAATCGA
    ATGGATGCAT TGGCAGCGAT TCAATGTTTC TGGCTTGTCC TGTTTGCGCT CAATAAAAGA
    GTCTTTGTTC GAAGAATATG GGTATTCTAT GTGATCTATA TGGCGATTCT GTATCCACTT
    CAGTTCTTTT CATATGTTGG ATTACCACCT GACAGTTGTA TTGAGTATCC ATGGTCGTAT
    TGGATTCCAT CGTATAGTGA TGATGCGAGA TTCAATCTGT CGTATCTTCT GAACTTGTCA
    ATATACGGTG TAAACTGGCC ATCAGCATAT CTCATTGGCG ATTTCTTCGT ACTCCTCTTG
    GCATCATGCC AATTAGCAGT TTTCCGGCGA GAAGGAGAAG ATAATGATTC AATTTACAAT
    GATGGAAACT TTGTGATTAA GCCAGAAAAT CCACAATATG ACTTTATTGA CACCAAAAAG
    AGTTACGTTG ATTATTTCAA ATCATTCGTT TTCCATTATG GACACTGGAT TACATTGATG
    TCTACACTAG CAGCAGGAAT TGCTGGAACG TCACTTTTTG CTTTGGGATA CATTATATTC
    ACTCTGACAA TGCTGTGGTC TGGTAACAAT TTGTATGTGA TGAACTCAAC TCTACGATCA
    TTTGAACATA CTTTGAAACG ATGGAATGCA CTTTTGGGAT ACACATTGTT CACGATTACA
    ATGAAAGTTT GCCTTCAAAT ATTTGGATGT GTATTCCTGT CGTGGTTCGA TCAATCTGGT
    GGATGGGGAA AGACTCTGTG CATCGTTCGT CAACTCTTCA GTATCACATG TGTTAATAAC
    GAATGTCATG TGCTCAAAGA GCTGGAGGAT TTCTCGAAGG CGTGTGCTGT TGAGACGAAA
    GAAGGAAATA TTGGATTTGA TGTGATTGCG CTATCCTTCC TCGTCTTCCA AATCCGAATT
    TTCCATTCTT GGTATTTCCA ACACTGCATG GTTGAATATC GAAGTGAGGT TATTCTGGCC
    AATAGAGGTG CCGTGCTGAA GAATCAACTG ATTGAAAAGG AAATGAAGGA ACAAAACGAG
    CAGCAAAAAG CCAAGTTCAA TGATATCAGA AGAAGAACTG AAGCTATTCG TGAAAGATAT
    CAAAAACAAA TCGAACGAGG AGCTGCTGAA CGTGACTTCG AGCCGGTTAC TTATGGGCAC
    GCCAAACGAG CGGGAGACTA CTACATGTTC AAATACGATC CTGAAAATGA TGATCTTGTG
    GAACCCGTCG ACAGTTTTGT ACCAGAAGTT GATCCCAAGG CAACTGCATA TGATCGACTT
    GATCCTGGAC AGATAATGTA CGCTGCCACC GCTCACGACC TCGACCTCGC CAAAACTGTA
    CAGCAAGTCA AGAAAGGCGA TACTATCAAA GATCCTGATT CACGTGCTCT TATCGCTGTT
    TCTGAACCAG AAGCTCGTAA GCCAGGAGGC ACTGAAGAAA CGGATGGTGA TGAAGATGAG
    GATAATAAGG ATTCGAAAGT GGAAAGTACT GCTAAATTCA TTCAAAAAAT GATCGCTTCT
    GCCTTGGACT TGTGTAGTGT AACACTAAAC AAACTGTGCA GAGAACACCG ATATGTTGGA
    TTTGTTTTGA GCAAGGAAAA ACAAAAGTTG AAGTCAGGAC ACTCGGAATC TCTTTCAAAT
    ACATCTAGAA AGCTGACGGA TATTCGATCT GCAGTTGATC TACCAAGTCT TCAACTTGTG
    CAATCGGCGA ATGATGTTGA AAAGATGGAG ACAGCCGTCA GCGTGGATTG GCAACAAAAA
    TCATCAGCAA CGAGACTATT GAATGCAGTT GTGAATTGTA TTGGAGCACA CACTGATATT
    CTCTGCTACT TCTTCGCAAT TATGACACAA GTGATGACTG GTGGACTTAT TACTCTTCCA
    CTTCCTCTGA TGTCACTATT CTGGGGAAAT CTATCGAATC CTCGTCCATC CAAGTTCTTC
    TGGGTCACAA TGATCACCTA CACTGAGTGT GTCATTGTTA TCAAGTTTGT TTGCCAATTT
    GCATTCATGC CTTACAATTC TATCACGTGG CGAACAGAGC ATCAAATGGA CCCGATGTCA
    TTGGATAAGT TATTCGGAGT ATCACAGAGA GATAGTTTTG CACTTTGGGA CATTGTTTTG
    CTCTTCTCAC TCTTCTTCCA TCGGTATATG CTCAGAAAGT TAGGGTTATG GAAGGATGCC
    AATTTGACGG ACACGTTCAC TTTGAAGGAA GAGCCTCGAT CGGCGTCAGG ATCGGACACA
    GGATCACCCA AGAAAATTGC TCAAGAGCCA AAGGTTGTTG TAACTCAGTC AGATACCCTT
    GAAGGTACAA GTGGAGGAGA GATAGTCATA CCATCTGATC CAAATGCAGT TTCCAATATG
    GAAGAATTAG ACTGCGAACC TCCGATTCCA GAGAAGCAAA GTGGACCAAT TGGAAGATTC
    ATTCATCAAT TATTCCATCC AAAATTCCGT TACATTCGTG ATTTGTATCC AATAATGTTT
    GGAATCGATG TGATATGCTT CCTCATCATG ACATTTGGAT ACTCGGCATT TGGTGAAGGA
    GGTTCTGGAA ATGTTTTAGA TGATGTGAAG GCTTCTCGTA TTCCGGTAAC ACTTGTTGTT
    ATGCTCGTTG GAATGACTCT GGCAATTATC ATTGATCGAG CACTCTACCT CAGAAAATCA
    GTTGTCGGAA AGCTAATCTA TCAAGTCTTA ATGATTGCTT TCCTTCACAT TTGGGTATTC
    CTTGTTCTTC CAAATATGAC TCGTCGTTCT GCGATATCGA ATCACGTTGC TCAAGCATTG
    TACGTCATCA AATCGTGCTA TTTCCTTGTG TCTGCTTGGC AGATCCGAAA TGGTTATCCT
    GAACTTTGTA TTGGAAATCT ATTAACACAT TCATACGGAA TGACCAATAT GATTGCATTC
    AAAGTATTCA TGAATATTCC ATTCCTCTTC GAGCTTCGTA CAGCTATTGA TTGGACATGG
    ACAGATACTT CAATGCCTCT TTTCGATTTC TTCAATATGG AAAATTTCTA TGCTCATATA
    TTCAATATCA AGTGTGCCCG CCAGTTCGAA GCTGCCTATC CAGCTCCAAG AGGGATTCCC
    AAAGGAAAGT TGGTCAAGTA TATGATGGGA TTCCCAATTA TCATTGGAGT CGTTATCTTC
    ATCTTTTCTC CTCTATTGCT CTGGTCGCTA CTCAATCAAA TCGGAACTAT CAGTATGCCA
    GAAAAAGTTA CGTTGAGGAT TTCGATTGAA GGATATCCAC CACTTTACGA GATGGAGGCA
    CAAGGAAGTA ATCACGATAA CGCTGAACTT GGAATGATAA AACCTGATCA ATTGGCATCT
    TTGAATCAAG CATTGACGGA TAGTTACACG ACACGAGACA CTAATTCTAT TTTAAGATCA
    AGAATGTCTG TATCATATCT GAAAGGATAC ACATATGAGG ATATTCTGAT TGTGCGATTC
    CGTCCAGAAT CTGAAATTTA TTGGCCGATT TCACAGGATT CGAGAAATGC GATGATCGAT
    AAATTGAGCC GAAACACGTC AGTCAATTTT GAAGTATCTC TGGAATTCAC TCGGCCATAT
    GATCCAAATG AAAACGCTGC TTTAAAACAT TCGAAATCGT GGCTTGTTCC AATCTCCCTG
    GATATGACGA TTCGAGCAAA AATTCAAAGT GCTCTCCGAG GAGATCCAGG TCATCCGATT
    CTCATTCCAC AGTCAATTCC TGCGTTTATT CAAGTTCCAA ATCAAGGAGA ATTGACCCTT
    CCAACATCAA TCGGAAATAC TATTATCAAT GACGGAAATC CACGGATTAA TACGACCGGG
    ATGGAAAAAT CAGACGAAGC TCGGGCTTGG TTCGACTCGT TAACCTTGAA TCTCGAACAA
    GGAAAATCGC AAAACGAGAA AATGTGGATC GCCACATCCG AACATCCAGG AGACCAAAAT
    GCGAAACTCT GGATAAAGAC TGCAAATACG ACGTATTCGG GAAGGCCGTA CCTTCAAGTT
    GTCGGATTTA TTGATAGAGC ATTCCCATCA TTTTTGGCGA AAGTTTTCAA AGGAGGAGTC
    ATTGCAGTGT ATCTTTCAGT TATCCTCGTC GTTGGAAGAG GTCTGGTTCG TGGAATCTTC
    ACCACATCTC CAAGCACTGT CATGTTCACA GAATTACCGA ATGCGGATCA TCTTCTCAAA
    ATTTGTCTCG ACATCTATTT GGTTCGAGAA GCGAAGGACT TCATGTTGGA GCAGGATCTC
    TTCGCCAAAT TAATATTCCT GTTCCGATCA CCAGCGACAC TCATCGAATG GACACGAATG
    AGCAAAAAGA AGCAAGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa356222
    Clone ID Related Accession (Same CDS sequence) NM_001268456.3 , NM_001268456.2
    Accession Version NM_001268456.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5115bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268456.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCTAACAA AGTCAATAGT GAGTAGTAGT GGTGACTCTG GTATTTACGA AGACGGTTGT 
    GGTATCATGC CGTACATTGA CGACGACGAT GATTTGACGC CAATTATAAT GGCAAGACCT
    GCTATTGGCA GTGGGTACAC GGGTGGCAGG AACGGATGGC ATAGATCACC CAGGCAGTAT
    TTGACCAACT GGTGGAGCGA TACCCTTGTC AAGAAGCTGC ACAAATTGGC AAATCAGACA
    ATCGAACTGT TATGGAGGTT TTTCGAAGTT CACATTTCGA AAATTGTCTT TGTCATCATT
    GCGATTTTTA TTGCCAACAA TATCAATGCT CTATACATTC CACTTGTTAT TCTCCTCTCA
    TTGGCTATAT GTCTACCATC AGCTGCAGAT GGGATATTCT CTTTATTTAT GTGTGCCTAC
    TTATTCCTCG TGGCACTGAG CAAGATGATC TACCAACTGG ATATTGTTCC CGAGTTATCA
    CAAATCGACC GAGGAGTTGG AGCAGATAAC TGTTCACATG GAAATATTTC AATGCCTGAA
    TGGTTTGGAT TGAAGAAGGA AGTTGAAGGA ACTGAACCAA TTTATATGCT CTTTGGAGTC
    ATTGTTTCGA TTATTGCATT GGCTTTTCAA TCAATTGTTA TATATCGACA AAGACATTAT
    CGAGCTTCAC TGGGACTTCC TGAATCGATG CGAGCTAAAG TGTTCCCGGA CTTCCATCAT
    TCTCATTTTG ATAGATCTCT CAAAAATGCC ATCCAATTCT TGATAGACTA TGGTTTCTAT
    AAGTTTGGAC TTGAAATCAC GATGATCGCC ATTGGAATTG ACATTTTCAA TCGAATGGAT
    GCATTGGCAG CGATTCAATG TTTCTGGCTT GTCCTGTTTG CGCTCAATAA AAGAGTCTTT
    GTTCGAAGAA TATGGGTATT CTATGTGATC TATATGGCGA TTCTGTATCC ACTTCAGTTC
    TTTTCATATG TTGGATTACC ACCTGACAGT TGTATTGAGT ATCCATGGTC GTATTGGATT
    CCATCGTATA GTGATGATGC GAGATTCAAT CTGTCGTATC TTCTGAACTT GTCAATATAC
    GGTGTAAACT GGCCATCAGC ATATCTCATT GGCGATTTCT TCGTACTCCT CTTGGCATCA
    TGCCAATTAG CAGTTTTCCG GCGAGAAGGA GAAGATAATG ATTCAATTTA CAATGATGGA
    AACTTTGTGA TTAAGCCAGA AAATCCACAA TATGACTTTA TTGACACCAA AAAGAGTTAC
    GTTGATTATT TCAAATCATT CGTTTTCCAT TATGGACACT GGATTACATT GATGTCTACA
    CTAGCAGCAG GAATTGCTGG AACGTCACTT TTTGCTTTGG GATACATTAT ATTCACTCTG
    ACAATGCTGT GGTCTGGTAA CAATTTGTAT GTGATGAACT CAACTCTACG ATCATTTGAA
    CATACTTTGA AACGATGGAA TGCACTTTTG GGATACACAT TGTTCACGAT TACAATGAAA
    GTTTGCCTTC AAATATTTGG ATGTGTATTC CTGTCGTGGT TCGATCAATC TGGTGGATGG
    GGAAAGACTC TGTGCATCGT TCGTCAACTC TTCAGTATCA CATGTGTTAA TAACGAATGT
    CATGTGCTCA AAGAGCTGGA GGATTTCTCG AAGGCGTGTG CTGTTGAGAC GAAAGAAGGA
    AATATTGGAT TTGATGTGAT TGCGCTATCC TTCCTCGTCT TCCAAATCCG AATTTTCCAT
    TCTTGGTATT TCCAACACTG CATGGTTGAA TATCGAAGTG AGGTTATTCT GGCCAATAGA
    GGTGCCGTGC TGAAGAATCA ACTGATTGAA AAGGAAATGA AGGAACAAAA CGAGCAGCAA
    AAAGCCAAGT TCAATGATAT CAGAAGAAGA ACTGAAGCTA TTCGTGAAAG ATATCAAAAA
    CAAATCGAAC GAGGAGCTGC TGAACGTGAC TTCGAGCCGG TTACTTATGG GCACGCCAAA
    CGAGCGGGAG ACTACTACAT GTTCAAATAC GATCCTGAAA ATGATGATCT TGTGGAACCC
    GTCGACAGTT TTGTACCAGA AGTTGATCCC AAGGCAACTG CATATGATCG ACTTGATCCT
    GGACAGATAA TGTACGCTGC CACCGCTCAC GACCTCGACC TCGCCAAAAC TGTACAGCAA
    GTCAAGAAAG GCGATACTAT CAAAGATCCT GATTCACGTG CTCTTATCGC TGTTTCTGAA
    CCAGAAGCTC GTAAGCCAGG AGGCACTGAA GAAACGGATG GTGATGAAGA TGAGGATAAT
    AAGGATTCGA AAGTGGAAAG TACTGCTAAA TTCATTCAAA AAATGATCGC TTCTGCCTTG
    GACTTGTGTA GTGTAACACT AAACAAACTG TGCAGAGAAC ACCGATATGT TGGATTTGTT
    TTGAGCAAGG AAAAACAAAA GTTGAAGTCA GGACACTCGG AATCTCTTTC AAATACATCT
    AGAAAGCTGA CGGATATTCG ATCTGCAGTT GATCTACCAA GTCTTCAACT TGTGCAATCG
    GCGAATGATG TTGAAAAGAT GGAGACAGCC GTCAGCGTGG ATTGGCAACA AAAATCATCA
    GCAACGAGAC TATTGAATGC AGTTGTGAAT TGTATTGGAG CACACACTGA TATTCTCTGC
    TACTTCTTCG CAATTATGAC ACAAGTGATG ACTGGTGGAC TTATTACTCT TCCACTTCCT
    CTGATGTCAC TATTCTGGGG AAATCTATCG AATCCTCGTC CATCCAAGTT CTTCTGGGTC
    ACAATGATCA CCTACACTGA GTGTGTCATT GTTATCAAGT TTGTTTGCCA ATTTGCATTC
    ATGCCTTACA ATTCTATCAC GTGGCGAACA GAGCATCAAA TGGACCCGAT GTCATTGGAT
    AAGTTATTCG GAGTATCACA GAGAGATAGT TTTGCACTTT GGGACATTGT TTTGCTCTTC
    TCACTCTTCT TCCATCGGTA TATGCTCAGA AAGTTAGGGT TATGGAAGGA TGCCAATTTG
    ACGGACACGT TCACTTTGAA GGAAGAGCCT CGATCGGCGT CAGGATCGGA CACAGGATCA
    CCCAAGAAAA TTGCTCAAGA GCCAAAGGTT GTTGTAACTC AGTCAGATAC CCTTGAAGGT
    ACAAGTGGAG GAGAGATAGT CATACCATCT GATCCAAATG CAGTTTCCAA TATGGAAGAA
    TTAGACTGCG AACCTCCGAT TCCAGAGAAG CAAAGTGGAC CAATTGGAAG ATTCATTCAT
    CAATTATTCC ATCCAAAATT CCGTTACATT CGTGATTTGT ATCCAATAAT GTTTGGAATC
    GATGTGATAT GCTTCCTCAT CATGACATTT GGATACTCGG CATTTGGTGA AGGAGGTTCT
    GGAAATGTTT TAGATGATGT GAAGGCTTCT CGTATTCCGG TAACACTTGT TGTTATGCTC
    GTTGGAATGA CTCTGGCAAT TATCATTGAT CGAGCACTCT ACCTCAGAAA ATCAGTTGTC
    GGAAAGCTAA TCTATCAAGT CTTAATGATT GCTTTCCTTC ACATTTGGGT ATTCCTTGTT
    CTTCCAAATA TGACTCGTCG TTCTGCGATA TCGAATCACG TTGCTCAAGC ATTGTACGTC
    ATCAAATCGT GCTATTTCCT TGTGTCTGCT TGGCAGATCC GAAATGGTTA TCCTGAACTT
    TGTATTGGAA ATCTATTAAC ACATTCATAC GGAATGACCA ATATGATTGC ATTCAAAGTA
    TTCATGAATA TTCCATTCCT CTTCGAGCTT CGTACAGCTA TTGATTGGAC ATGGACAGAT
    ACTTCAATGC CTCTTTTCGA TTTCTTCAAT ATGGAAAATT TCTATGCTCA TATATTCAAT
    ATCAAGTGTG CCCGCCAGTT CGAAGCTGCC TATCCAGCTC CAAGAGGGAT TCCCAAAGGA
    AAGTTGGTCA AGTATATGAT GGGATTCCCA ATTATCATTG GAGTCGTTAT CTTCATCTTT
    TCTCCTCTAT TGCTCTGGTC GCTACTCAAT CAAATCGGAA CTATCAGTAT GCCAGAAAAA
    GTTACGTTGA GGATTTCGAT TGAAGGATAT CCACCACTTT ACGAGATGGA GGCACAAGGA
    AGTAATCACG ATAACGCTGA ACTTGGAATG ATAAAACCTG ATCAATTGGC ATCTTTGAAT
    CAAGCATTGA CGGATAGTTA CACGACACGA GACACTAATT CTATTTTAAG ATCAAGAATG
    TCTGTATCAT ATCTGAAAGG ATACACATAT GAGGATATTC TGATTGTGCG ATTCCGTCCA
    GAATCTGAAA TTTATTGGCC GATTTCACAG GATTCGAGAA ATGCGATGAT CGATAAATTG
    AGCCGAAACA CGTCAGTCAA TTTTGAAGTA TCTCTGGAAT TCACTCGGCC ATATGATCCA
    AATGAAAACG CTGCTTTAAA ACATTCGAAA TCGTGGCTTG TTCCAATCTC CCTGGATATG
    ACGATTCGAG CAAAAATTCA AAGTGCTCTC CGAGGAGATC CAGGTCATCC GATTCTCATT
    CCACAGTCAA TTCCTGCGTT TATTCAAGTT CCAAATCAAG GAGAATTGAC CCTTCCAACA
    TCAATCGGAA ATACTATTAT CAATGACGGA AATCCACGGA TTAATACGAC CGGGATGGAA
    AAATCAGACG AAGCTCGGGC TTGGTTCGAC TCGTTAACCT TGAATCTCGA ACAAGGAAAA
    TCGCAAAACG AGAAAATGTG GATCGCCACA TCCGAACATC CAGGAGACCA AAATGCGAAA
    CTCTGGATAA AGACTGCAAA TACGACGTAT TCGGGAAGGC CGTACCTTCA AGTTGTCGGA
    TTTATTGATA GAGCATTCCC ATCATTTTTG GCGAAAGTTT TCAAAGGAGG AGTCATTGCA
    GTGTATCTTT CAGTTATCCT CGTCGTTGGA AGAGGTCTGG TTCGTGGAAT CTTCACCACA
    TCTCCAAGCA CTGTCATGTT CACAGAATTA CCGAATGCGG ATCATCTTCT CAAAATTTGT
    CTCGACATCT ATTTGGTTCG AGAAGCGAAG GACTTCATGT TGGAGCAGGA TCTCTTCGCC
    AAATTAATAT TCCTGTTCCG ATCACCAGCG ACACTCATCG AATGGACACG AATGAGCAAA
    AAGAAGCAAG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255385.2
    CDS349..5463
    Translation

    Target ORF information:

    RefSeq Version NM_001268456.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268456.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCTAACAA AGTCAATAGT GAGTAGTAGT GGTGACTCTG GTATTTACGA AGACGGTTGT 
    GGTATCATGC CGTACATTGA CGACGACGAT GATTTGACGC CAATTATAAT GGCAAGACCT
    GCTATTGGCA GTGGGTACAC GGGTGGCAGG AACGGATGGC ATAGATCACC CAGGCAGTAT
    TTGACCAACT GGTGGAGCGA TACCCTTGTC AAGAAGCTGC ACAAATTGGC AAATCAGACA
    ATCGAACTGT TATGGAGGTT TTTCGAAGTT CACATTTCGA AAATTGTCTT TGTCATCATT
    GCGATTTTTA TTGCCAACAA TATCAATGCT CTATACATTC CACTTGTTAT TCTCCTCTCA
    TTGGCTATAT GTCTACCATC AGCTGCAGAT GGGATATTCT CTTTATTTAT GTGTGCCTAC
    TTATTCCTCG TGGCACTGAG CAAGATGATC TACCAACTGG ATATTGTTCC CGAGTTATCA
    CAAATCGACC GAGGAGTTGG AGCAGATAAC TGTTCACATG GAAATATTTC AATGCCTGAA
    TGGTTTGGAT TGAAGAAGGA AGTTGAAGGA ACTGAACCAA TTTATATGCT CTTTGGAGTC
    ATTGTTTCGA TTATTGCATT GGCTTTTCAA TCAATTGTTA TATATCGACA AAGACATTAT
    CGAGCTTCAC TGGGACTTCC TGAATCGATG CGAGCTAAAG TGTTCCCGGA CTTCCATCAT
    TCTCATTTTG ATAGATCTCT CAAAAATGCC ATCCAATTCT TGATAGACTA TGGTTTCTAT
    AAGTTTGGAC TTGAAATCAC GATGATCGCC ATTGGAATTG ACATTTTCAA TCGAATGGAT
    GCATTGGCAG CGATTCAATG TTTCTGGCTT GTCCTGTTTG CGCTCAATAA AAGAGTCTTT
    GTTCGAAGAA TATGGGTATT CTATGTGATC TATATGGCGA TTCTGTATCC ACTTCAGTTC
    TTTTCATATG TTGGATTACC ACCTGACAGT TGTATTGAGT ATCCATGGTC GTATTGGATT
    CCATCGTATA GTGATGATGC GAGATTCAAT CTGTCGTATC TTCTGAACTT GTCAATATAC
    GGTGTAAACT GGCCATCAGC ATATCTCATT GGCGATTTCT TCGTACTCCT CTTGGCATCA
    TGCCAATTAG CAGTTTTCCG GCGAGAAGGA GAAGATAATG ATTCAATTTA CAATGATGGA
    AACTTTGTGA TTAAGCCAGA AAATCCACAA TATGACTTTA TTGACACCAA AAAGAGTTAC
    GTTGATTATT TCAAATCATT CGTTTTCCAT TATGGACACT GGATTACATT GATGTCTACA
    CTAGCAGCAG GAATTGCTGG AACGTCACTT TTTGCTTTGG GATACATTAT ATTCACTCTG
    ACAATGCTGT GGTCTGGTAA CAATTTGTAT GTGATGAACT CAACTCTACG ATCATTTGAA
    CATACTTTGA AACGATGGAA TGCACTTTTG GGATACACAT TGTTCACGAT TACAATGAAA
    GTTTGCCTTC AAATATTTGG ATGTGTATTC CTGTCGTGGT TCGATCAATC TGGTGGATGG
    GGAAAGACTC TGTGCATCGT TCGTCAACTC TTCAGTATCA CATGTGTTAA TAACGAATGT
    CATGTGCTCA AAGAGCTGGA GGATTTCTCG AAGGCGTGTG CTGTTGAGAC GAAAGAAGGA
    AATATTGGAT TTGATGTGAT TGCGCTATCC TTCCTCGTCT TCCAAATCCG AATTTTCCAT
    TCTTGGTATT TCCAACACTG CATGGTTGAA TATCGAAGTG AGGTTATTCT GGCCAATAGA
    GGTGCCGTGC TGAAGAATCA ACTGATTGAA AAGGAAATGA AGGAACAAAA CGAGCAGCAA
    AAAGCCAAGT TCAATGATAT CAGAAGAAGA ACTGAAGCTA TTCGTGAAAG ATATCAAAAA
    CAAATCGAAC GAGGAGCTGC TGAACGTGAC TTCGAGCCGG TTACTTATGG GCACGCCAAA
    CGAGCGGGAG ACTACTACAT GTTCAAATAC GATCCTGAAA ATGATGATCT TGTGGAACCC
    GTCGACAGTT TTGTACCAGA AGTTGATCCC AAGGCAACTG CATATGATCG ACTTGATCCT
    GGACAGATAA TGTACGCTGC CACCGCTCAC GACCTCGACC TCGCCAAAAC TGTACAGCAA
    GTCAAGAAAG GCGATACTAT CAAAGATCCT GATTCACGTG CTCTTATCGC TGTTTCTGAA
    CCAGAAGCTC GTAAGCCAGG AGGCACTGAA GAAACGGATG GTGATGAAGA TGAGGATAAT
    AAGGATTCGA AAGTGGAAAG TACTGCTAAA TTCATTCAAA AAATGATCGC TTCTGCCTTG
    GACTTGTGTA GTGTAACACT AAACAAACTG TGCAGAGAAC ACCGATATGT TGGATTTGTT
    TTGAGCAAGG AAAAACAAAA GTTGAAGTCA GGACACTCGG AATCTCTTTC AAATACATCT
    AGAAAGCTGA CGGATATTCG ATCTGCAGTT GATCTACCAA GTCTTCAACT TGTGCAATCG
    GCGAATGATG TTGAAAAGAT GGAGACAGCC GTCAGCGTGG ATTGGCAACA AAAATCATCA
    GCAACGAGAC TATTGAATGC AGTTGTGAAT TGTATTGGAG CACACACTGA TATTCTCTGC
    TACTTCTTCG CAATTATGAC ACAAGTGATG ACTGGTGGAC TTATTACTCT TCCACTTCCT
    CTGATGTCAC TATTCTGGGG AAATCTATCG AATCCTCGTC CATCCAAGTT CTTCTGGGTC
    ACAATGATCA CCTACACTGA GTGTGTCATT GTTATCAAGT TTGTTTGCCA ATTTGCATTC
    ATGCCTTACA ATTCTATCAC GTGGCGAACA GAGCATCAAA TGGACCCGAT GTCATTGGAT
    AAGTTATTCG GAGTATCACA GAGAGATAGT TTTGCACTTT GGGACATTGT TTTGCTCTTC
    TCACTCTTCT TCCATCGGTA TATGCTCAGA AAGTTAGGGT TATGGAAGGA TGCCAATTTG
    ACGGACACGT TCACTTTGAA GGAAGAGCCT CGATCGGCGT CAGGATCGGA CACAGGATCA
    CCCAAGAAAA TTGCTCAAGA GCCAAAGGTT GTTGTAACTC AGTCAGATAC CCTTGAAGGT
    ACAAGTGGAG GAGAGATAGT CATACCATCT GATCCAAATG CAGTTTCCAA TATGGAAGAA
    TTAGACTGCG AACCTCCGAT TCCAGAGAAG CAAAGTGGAC CAATTGGAAG ATTCATTCAT
    CAATTATTCC ATCCAAAATT CCGTTACATT CGTGATTTGT ATCCAATAAT GTTTGGAATC
    GATGTGATAT GCTTCCTCAT CATGACATTT GGATACTCGG CATTTGGTGA AGGAGGTTCT
    GGAAATGTTT TAGATGATGT GAAGGCTTCT CGTATTCCGG TAACACTTGT TGTTATGCTC
    GTTGGAATGA CTCTGGCAAT TATCATTGAT CGAGCACTCT ACCTCAGAAA ATCAGTTGTC
    GGAAAGCTAA TCTATCAAGT CTTAATGATT GCTTTCCTTC ACATTTGGGT ATTCCTTGTT
    CTTCCAAATA TGACTCGTCG TTCTGCGATA TCGAATCACG TTGCTCAAGC ATTGTACGTC
    ATCAAATCGT GCTATTTCCT TGTGTCTGCT TGGCAGATCC GAAATGGTTA TCCTGAACTT
    TGTATTGGAA ATCTATTAAC ACATTCATAC GGAATGACCA ATATGATTGC ATTCAAAGTA
    TTCATGAATA TTCCATTCCT CTTCGAGCTT CGTACAGCTA TTGATTGGAC ATGGACAGAT
    ACTTCAATGC CTCTTTTCGA TTTCTTCAAT ATGGAAAATT TCTATGCTCA TATATTCAAT
    ATCAAGTGTG CCCGCCAGTT CGAAGCTGCC TATCCAGCTC CAAGAGGGAT TCCCAAAGGA
    AAGTTGGTCA AGTATATGAT GGGATTCCCA ATTATCATTG GAGTCGTTAT CTTCATCTTT
    TCTCCTCTAT TGCTCTGGTC GCTACTCAAT CAAATCGGAA CTATCAGTAT GCCAGAAAAA
    GTTACGTTGA GGATTTCGAT TGAAGGATAT CCACCACTTT ACGAGATGGA GGCACAAGGA
    AGTAATCACG ATAACGCTGA ACTTGGAATG ATAAAACCTG ATCAATTGGC ATCTTTGAAT
    CAAGCATTGA CGGATAGTTA CACGACACGA GACACTAATT CTATTTTAAG ATCAAGAATG
    TCTGTATCAT ATCTGAAAGG ATACACATAT GAGGATATTC TGATTGTGCG ATTCCGTCCA
    GAATCTGAAA TTTATTGGCC GATTTCACAG GATTCGAGAA ATGCGATGAT CGATAAATTG
    AGCCGAAACA CGTCAGTCAA TTTTGAAGTA TCTCTGGAAT TCACTCGGCC ATATGATCCA
    AATGAAAACG CTGCTTTAAA ACATTCGAAA TCGTGGCTTG TTCCAATCTC CCTGGATATG
    ACGATTCGAG CAAAAATTCA AAGTGCTCTC CGAGGAGATC CAGGTCATCC GATTCTCATT
    CCACAGTCAA TTCCTGCGTT TATTCAAGTT CCAAATCAAG GAGAATTGAC CCTTCCAACA
    TCAATCGGAA ATACTATTAT CAATGACGGA AATCCACGGA TTAATACGAC CGGGATGGAA
    AAATCAGACG AAGCTCGGGC TTGGTTCGAC TCGTTAACCT TGAATCTCGA ACAAGGAAAA
    TCGCAAAACG AGAAAATGTG GATCGCCACA TCCGAACATC CAGGAGACCA AAATGCGAAA
    CTCTGGATAA AGACTGCAAA TACGACGTAT TCGGGAAGGC CGTACCTTCA AGTTGTCGGA
    TTTATTGATA GAGCATTCCC ATCATTTTTG GCGAAAGTTT TCAAAGGAGG AGTCATTGCA
    GTGTATCTTT CAGTTATCCT CGTCGTTGGA AGAGGTCTGG TTCGTGGAAT CTTCACCACA
    TCTCCAAGCA CTGTCATGTT CACAGAATTA CCGAATGCGG ATCATCTTCT CAAAATTTGT
    CTCGACATCT ATTTGGTTCG AGAAGCGAAG GACTTCATGT TGGAGCAGGA TCTCTTCGCC
    AAATTAATAT TCCTGTTCCG ATCACCAGCG ACACTCATCG AATGGACACG AATGAGCAAA
    AAGAAGCAAG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa356222
    Clone ID Related Accession (Same CDS sequence) NM_001268456.3 , NM_001268456.2
    Accession Version NM_001268456.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5115bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Apr 20, 2015 this sequence version replaced NM_001268456.1. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCTAACAA AGTCAATAGT GAGTAGTAGT GGTGACTCTG GTATTTACGA AGACGGTTGT 
    GGTATCATGC CGTACATTGA CGACGACGAT GATTTGACGC CAATTATAAT GGCAAGACCT
    GCTATTGGCA GTGGGTACAC GGGTGGCAGG AACGGATGGC ATAGATCACC CAGGCAGTAT
    TTGACCAACT GGTGGAGCGA TACCCTTGTC AAGAAGCTGC ACAAATTGGC AAATCAGACA
    ATCGAACTGT TATGGAGGTT TTTCGAAGTT CACATTTCGA AAATTGTCTT TGTCATCATT
    GCGATTTTTA TTGCCAACAA TATCAATGCT CTATACATTC CACTTGTTAT TCTCCTCTCA
    TTGGCTATAT GTCTACCATC AGCTGCAGAT GGGATATTCT CTTTATTTAT GTGTGCCTAC
    TTATTCCTCG TGGCACTGAG CAAGATGATC TACCAACTGG ATATTGTTCC CGAGTTATCA
    CAAATCGACC GAGGAGTTGG AGCAGATAAC TGTTCACATG GAAATATTTC AATGCCTGAA
    TGGTTTGGAT TGAAGAAGGA AGTTGAAGGA ACTGAACCAA TTTATATGCT CTTTGGAGTC
    ATTGTTTCGA TTATTGCATT GGCTTTTCAA TCAATTGTTA TATATCGACA AAGACATTAT
    CGAGCTTCAC TGGGACTTCC TGAATCGATG CGAGCTAAAG TGTTCCCGGA CTTCCATCAT
    TCTCATTTTG ATAGATCTCT CAAAAATGCC ATCCAATTCT TGATAGACTA TGGTTTCTAT
    AAGTTTGGAC TTGAAATCAC GATGATCGCC ATTGGAATTG ACATTTTCAA TCGAATGGAT
    GCATTGGCAG CGATTCAATG TTTCTGGCTT GTCCTGTTTG CGCTCAATAA AAGAGTCTTT
    GTTCGAAGAA TATGGGTATT CTATGTGATC TATATGGCGA TTCTGTATCC ACTTCAGTTC
    TTTTCATATG TTGGATTACC ACCTGACAGT TGTATTGAGT ATCCATGGTC GTATTGGATT
    CCATCGTATA GTGATGATGC GAGATTCAAT CTGTCGTATC TTCTGAACTT GTCAATATAC
    GGTGTAAACT GGCCATCAGC ATATCTCATT GGCGATTTCT TCGTACTCCT CTTGGCATCA
    TGCCAATTAG CAGTTTTCCG GCGAGAAGGA GAAGATAATG ATTCAATTTA CAATGATGGA
    AACTTTGTGA TTAAGCCAGA AAATCCACAA TATGACTTTA TTGACACCAA AAAGAGTTAC
    GTTGATTATT TCAAATCATT CGTTTTCCAT TATGGACACT GGATTACATT GATGTCTACA
    CTAGCAGCAG GAATTGCTGG AACGTCACTT TTTGCTTTGG GATACATTAT ATTCACTCTG
    ACAATGCTGT GGTCTGGTAA CAATTTGTAT GTGATGAACT CAACTCTACG ATCATTTGAA
    CATACTTTGA AACGATGGAA TGCACTTTTG GGATACACAT TGTTCACGAT TACAATGAAA
    GTTTGCCTTC AAATATTTGG ATGTGTATTC CTGTCGTGGT TCGATCAATC TGGTGGATGG
    GGAAAGACTC TGTGCATCGT TCGTCAACTC TTCAGTATCA CATGTGTTAA TAACGAATGT
    CATGTGCTCA AAGAGCTGGA GGATTTCTCG AAGGCGTGTG CTGTTGAGAC GAAAGAAGGA
    AATATTGGAT TTGATGTGAT TGCGCTATCC TTCCTCGTCT TCCAAATCCG AATTTTCCAT
    TCTTGGTATT TCCAACACTG CATGGTTGAA TATCGAAGTG AGGTTATTCT GGCCAATAGA
    GGTGCCGTGC TGAAGAATCA ACTGATTGAA AAGGAAATGA AGGAACAAAA CGAGCAGCAA
    AAAGCCAAGT TCAATGATAT CAGAAGAAGA ACTGAAGCTA TTCGTGAAAG ATATCAAAAA
    CAAATCGAAC GAGGAGCTGC TGAACGTGAC TTCGAGCCGG TTACTTATGG GCACGCCAAA
    CGAGCGGGAG ACTACTACAT GTTCAAATAC GATCCTGAAA ATGATGATCT TGTGGAACCC
    GTCGACAGTT TTGTACCAGA AGTTGATCCC AAGGCAACTG CATATGATCG ACTTGATCCT
    GGACAGATAA TGTACGCTGC CACCGCTCAC GACCTCGACC TCGCCAAAAC TGTACAGCAA
    GTCAAGAAAG GCGATACTAT CAAAGATCCT GATTCACGTG CTCTTATCGC TGTTTCTGAA
    CCAGAAGCTC GTAAGCCAGG AGGCACTGAA GAAACGGATG GTGATGAAGA TGAGGATAAT
    AAGGATTCGA AAGTGGAAAG TACTGCTAAA TTCATTCAAA AAATGATCGC TTCTGCCTTG
    GACTTGTGTA GTGTAACACT AAACAAACTG TGCAGAGAAC ACCGATATGT TGGATTTGTT
    TTGAGCAAGG AAAAACAAAA GTTGAAGTCA GGACACTCGG AATCTCTTTC AAATACATCT
    AGAAAGCTGA CGGATATTCG ATCTGCAGTT GATCTACCAA GTCTTCAACT TGTGCAATCG
    GCGAATGATG TTGAAAAGAT GGAGACAGCC GTCAGCGTGG ATTGGCAACA AAAATCATCA
    GCAACGAGAC TATTGAATGC AGTTGTGAAT TGTATTGGAG CACACACTGA TATTCTCTGC
    TACTTCTTCG CAATTATGAC ACAAGTGATG ACTGGTGGAC TTATTACTCT TCCACTTCCT
    CTGATGTCAC TATTCTGGGG AAATCTATCG AATCCTCGTC CATCCAAGTT CTTCTGGGTC
    ACAATGATCA CCTACACTGA GTGTGTCATT GTTATCAAGT TTGTTTGCCA ATTTGCATTC
    ATGCCTTACA ATTCTATCAC GTGGCGAACA GAGCATCAAA TGGACCCGAT GTCATTGGAT
    AAGTTATTCG GAGTATCACA GAGAGATAGT TTTGCACTTT GGGACATTGT TTTGCTCTTC
    TCACTCTTCT TCCATCGGTA TATGCTCAGA AAGTTAGGGT TATGGAAGGA TGCCAATTTG
    ACGGACACGT TCACTTTGAA GGAAGAGCCT CGATCGGCGT CAGGATCGGA CACAGGATCA
    CCCAAGAAAA TTGCTCAAGA GCCAAAGGTT GTTGTAACTC AGTCAGATAC CCTTGAAGGT
    ACAAGTGGAG GAGAGATAGT CATACCATCT GATCCAAATG CAGTTTCCAA TATGGAAGAA
    TTAGACTGCG AACCTCCGAT TCCAGAGAAG CAAAGTGGAC CAATTGGAAG ATTCATTCAT
    CAATTATTCC ATCCAAAATT CCGTTACATT CGTGATTTGT ATCCAATAAT GTTTGGAATC
    GATGTGATAT GCTTCCTCAT CATGACATTT GGATACTCGG CATTTGGTGA AGGAGGTTCT
    GGAAATGTTT TAGATGATGT GAAGGCTTCT CGTATTCCGG TAACACTTGT TGTTATGCTC
    GTTGGAATGA CTCTGGCAAT TATCATTGAT CGAGCACTCT ACCTCAGAAA ATCAGTTGTC
    GGAAAGCTAA TCTATCAAGT CTTAATGATT GCTTTCCTTC ACATTTGGGT ATTCCTTGTT
    CTTCCAAATA TGACTCGTCG TTCTGCGATA TCGAATCACG TTGCTCAAGC ATTGTACGTC
    ATCAAATCGT GCTATTTCCT TGTGTCTGCT TGGCAGATCC GAAATGGTTA TCCTGAACTT
    TGTATTGGAA ATCTATTAAC ACATTCATAC GGAATGACCA ATATGATTGC ATTCAAAGTA
    TTCATGAATA TTCCATTCCT CTTCGAGCTT CGTACAGCTA TTGATTGGAC ATGGACAGAT
    ACTTCAATGC CTCTTTTCGA TTTCTTCAAT ATGGAAAATT TCTATGCTCA TATATTCAAT
    ATCAAGTGTG CCCGCCAGTT CGAAGCTGCC TATCCAGCTC CAAGAGGGAT TCCCAAAGGA
    AAGTTGGTCA AGTATATGAT GGGATTCCCA ATTATCATTG GAGTCGTTAT CTTCATCTTT
    TCTCCTCTAT TGCTCTGGTC GCTACTCAAT CAAATCGGAA CTATCAGTAT GCCAGAAAAA
    GTTACGTTGA GGATTTCGAT TGAAGGATAT CCACCACTTT ACGAGATGGA GGCACAAGGA
    AGTAATCACG ATAACGCTGA ACTTGGAATG ATAAAACCTG ATCAATTGGC ATCTTTGAAT
    CAAGCATTGA CGGATAGTTA CACGACACGA GACACTAATT CTATTTTAAG ATCAAGAATG
    TCTGTATCAT ATCTGAAAGG ATACACATAT GAGGATATTC TGATTGTGCG ATTCCGTCCA
    GAATCTGAAA TTTATTGGCC GATTTCACAG GATTCGAGAA ATGCGATGAT CGATAAATTG
    AGCCGAAACA CGTCAGTCAA TTTTGAAGTA TCTCTGGAAT TCACTCGGCC ATATGATCCA
    AATGAAAACG CTGCTTTAAA ACATTCGAAA TCGTGGCTTG TTCCAATCTC CCTGGATATG
    ACGATTCGAG CAAAAATTCA AAGTGCTCTC CGAGGAGATC CAGGTCATCC GATTCTCATT
    CCACAGTCAA TTCCTGCGTT TATTCAAGTT CCAAATCAAG GAGAATTGAC CCTTCCAACA
    TCAATCGGAA ATACTATTAT CAATGACGGA AATCCACGGA TTAATACGAC CGGGATGGAA
    AAATCAGACG AAGCTCGGGC TTGGTTCGAC TCGTTAACCT TGAATCTCGA ACAAGGAAAA
    TCGCAAAACG AGAAAATGTG GATCGCCACA TCCGAACATC CAGGAGACCA AAATGCGAAA
    CTCTGGATAA AGACTGCAAA TACGACGTAT TCGGGAAGGC CGTACCTTCA AGTTGTCGGA
    TTTATTGATA GAGCATTCCC ATCATTTTTG GCGAAAGTTT TCAAAGGAGG AGTCATTGCA
    GTGTATCTTT CAGTTATCCT CGTCGTTGGA AGAGGTCTGG TTCGTGGAAT CTTCACCACA
    TCTCCAAGCA CTGTCATGTT CACAGAATTA CCGAATGCGG ATCATCTTCT CAAAATTTGT
    CTCGACATCT ATTTGGTTCG AGAAGCGAAG GACTTCATGT TGGAGCAGGA TCTCTTCGCC
    AAATTAATAT TCCTGTTCCG ATCACCAGCG ACACTCATCG AATGGACACG AATGAGCAAA
    AAGAAGCAAG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255385.2
    CDS1..5115
    Translation

    Target ORF information:

    RefSeq Version NM_001268456.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268456.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCTAACAA AGTCAATAGT GAGTAGTAGT GGTGACTCTG GTATTTACGA AGACGGTTGT 
    GGTATCATGC CGTACATTGA CGACGACGAT GATTTGACGC CAATTATAAT GGCAAGACCT
    GCTATTGGCA GTGGGTACAC GGGTGGCAGG AACGGATGGC ATAGATCACC CAGGCAGTAT
    TTGACCAACT GGTGGAGCGA TACCCTTGTC AAGAAGCTGC ACAAATTGGC AAATCAGACA
    ATCGAACTGT TATGGAGGTT TTTCGAAGTT CACATTTCGA AAATTGTCTT TGTCATCATT
    GCGATTTTTA TTGCCAACAA TATCAATGCT CTATACATTC CACTTGTTAT TCTCCTCTCA
    TTGGCTATAT GTCTACCATC AGCTGCAGAT GGGATATTCT CTTTATTTAT GTGTGCCTAC
    TTATTCCTCG TGGCACTGAG CAAGATGATC TACCAACTGG ATATTGTTCC CGAGTTATCA
    CAAATCGACC GAGGAGTTGG AGCAGATAAC TGTTCACATG GAAATATTTC AATGCCTGAA
    TGGTTTGGAT TGAAGAAGGA AGTTGAAGGA ACTGAACCAA TTTATATGCT CTTTGGAGTC
    ATTGTTTCGA TTATTGCATT GGCTTTTCAA TCAATTGTTA TATATCGACA AAGACATTAT
    CGAGCTTCAC TGGGACTTCC TGAATCGATG CGAGCTAAAG TGTTCCCGGA CTTCCATCAT
    TCTCATTTTG ATAGATCTCT CAAAAATGCC ATCCAATTCT TGATAGACTA TGGTTTCTAT
    AAGTTTGGAC TTGAAATCAC GATGATCGCC ATTGGAATTG ACATTTTCAA TCGAATGGAT
    GCATTGGCAG CGATTCAATG TTTCTGGCTT GTCCTGTTTG CGCTCAATAA AAGAGTCTTT
    GTTCGAAGAA TATGGGTATT CTATGTGATC TATATGGCGA TTCTGTATCC ACTTCAGTTC
    TTTTCATATG TTGGATTACC ACCTGACAGT TGTATTGAGT ATCCATGGTC GTATTGGATT
    CCATCGTATA GTGATGATGC GAGATTCAAT CTGTCGTATC TTCTGAACTT GTCAATATAC
    GGTGTAAACT GGCCATCAGC ATATCTCATT GGCGATTTCT TCGTACTCCT CTTGGCATCA
    TGCCAATTAG CAGTTTTCCG GCGAGAAGGA GAAGATAATG ATTCAATTTA CAATGATGGA
    AACTTTGTGA TTAAGCCAGA AAATCCACAA TATGACTTTA TTGACACCAA AAAGAGTTAC
    GTTGATTATT TCAAATCATT CGTTTTCCAT TATGGACACT GGATTACATT GATGTCTACA
    CTAGCAGCAG GAATTGCTGG AACGTCACTT TTTGCTTTGG GATACATTAT ATTCACTCTG
    ACAATGCTGT GGTCTGGTAA CAATTTGTAT GTGATGAACT CAACTCTACG ATCATTTGAA
    CATACTTTGA AACGATGGAA TGCACTTTTG GGATACACAT TGTTCACGAT TACAATGAAA
    GTTTGCCTTC AAATATTTGG ATGTGTATTC CTGTCGTGGT TCGATCAATC TGGTGGATGG
    GGAAAGACTC TGTGCATCGT TCGTCAACTC TTCAGTATCA CATGTGTTAA TAACGAATGT
    CATGTGCTCA AAGAGCTGGA GGATTTCTCG AAGGCGTGTG CTGTTGAGAC GAAAGAAGGA
    AATATTGGAT TTGATGTGAT TGCGCTATCC TTCCTCGTCT TCCAAATCCG AATTTTCCAT
    TCTTGGTATT TCCAACACTG CATGGTTGAA TATCGAAGTG AGGTTATTCT GGCCAATAGA
    GGTGCCGTGC TGAAGAATCA ACTGATTGAA AAGGAAATGA AGGAACAAAA CGAGCAGCAA
    AAAGCCAAGT TCAATGATAT CAGAAGAAGA ACTGAAGCTA TTCGTGAAAG ATATCAAAAA
    CAAATCGAAC GAGGAGCTGC TGAACGTGAC TTCGAGCCGG TTACTTATGG GCACGCCAAA
    CGAGCGGGAG ACTACTACAT GTTCAAATAC GATCCTGAAA ATGATGATCT TGTGGAACCC
    GTCGACAGTT TTGTACCAGA AGTTGATCCC AAGGCAACTG CATATGATCG ACTTGATCCT
    GGACAGATAA TGTACGCTGC CACCGCTCAC GACCTCGACC TCGCCAAAAC TGTACAGCAA
    GTCAAGAAAG GCGATACTAT CAAAGATCCT GATTCACGTG CTCTTATCGC TGTTTCTGAA
    CCAGAAGCTC GTAAGCCAGG AGGCACTGAA GAAACGGATG GTGATGAAGA TGAGGATAAT
    AAGGATTCGA AAGTGGAAAG TACTGCTAAA TTCATTCAAA AAATGATCGC TTCTGCCTTG
    GACTTGTGTA GTGTAACACT AAACAAACTG TGCAGAGAAC ACCGATATGT TGGATTTGTT
    TTGAGCAAGG AAAAACAAAA GTTGAAGTCA GGACACTCGG AATCTCTTTC AAATACATCT
    AGAAAGCTGA CGGATATTCG ATCTGCAGTT GATCTACCAA GTCTTCAACT TGTGCAATCG
    GCGAATGATG TTGAAAAGAT GGAGACAGCC GTCAGCGTGG ATTGGCAACA AAAATCATCA
    GCAACGAGAC TATTGAATGC AGTTGTGAAT TGTATTGGAG CACACACTGA TATTCTCTGC
    TACTTCTTCG CAATTATGAC ACAAGTGATG ACTGGTGGAC TTATTACTCT TCCACTTCCT
    CTGATGTCAC TATTCTGGGG AAATCTATCG AATCCTCGTC CATCCAAGTT CTTCTGGGTC
    ACAATGATCA CCTACACTGA GTGTGTCATT GTTATCAAGT TTGTTTGCCA ATTTGCATTC
    ATGCCTTACA ATTCTATCAC GTGGCGAACA GAGCATCAAA TGGACCCGAT GTCATTGGAT
    AAGTTATTCG GAGTATCACA GAGAGATAGT TTTGCACTTT GGGACATTGT TTTGCTCTTC
    TCACTCTTCT TCCATCGGTA TATGCTCAGA AAGTTAGGGT TATGGAAGGA TGCCAATTTG
    ACGGACACGT TCACTTTGAA GGAAGAGCCT CGATCGGCGT CAGGATCGGA CACAGGATCA
    CCCAAGAAAA TTGCTCAAGA GCCAAAGGTT GTTGTAACTC AGTCAGATAC CCTTGAAGGT
    ACAAGTGGAG GAGAGATAGT CATACCATCT GATCCAAATG CAGTTTCCAA TATGGAAGAA
    TTAGACTGCG AACCTCCGAT TCCAGAGAAG CAAAGTGGAC CAATTGGAAG ATTCATTCAT
    CAATTATTCC ATCCAAAATT CCGTTACATT CGTGATTTGT ATCCAATAAT GTTTGGAATC
    GATGTGATAT GCTTCCTCAT CATGACATTT GGATACTCGG CATTTGGTGA AGGAGGTTCT
    GGAAATGTTT TAGATGATGT GAAGGCTTCT CGTATTCCGG TAACACTTGT TGTTATGCTC
    GTTGGAATGA CTCTGGCAAT TATCATTGAT CGAGCACTCT ACCTCAGAAA ATCAGTTGTC
    GGAAAGCTAA TCTATCAAGT CTTAATGATT GCTTTCCTTC ACATTTGGGT ATTCCTTGTT
    CTTCCAAATA TGACTCGTCG TTCTGCGATA TCGAATCACG TTGCTCAAGC ATTGTACGTC
    ATCAAATCGT GCTATTTCCT TGTGTCTGCT TGGCAGATCC GAAATGGTTA TCCTGAACTT
    TGTATTGGAA ATCTATTAAC ACATTCATAC GGAATGACCA ATATGATTGC ATTCAAAGTA
    TTCATGAATA TTCCATTCCT CTTCGAGCTT CGTACAGCTA TTGATTGGAC ATGGACAGAT
    ACTTCAATGC CTCTTTTCGA TTTCTTCAAT ATGGAAAATT TCTATGCTCA TATATTCAAT
    ATCAAGTGTG CCCGCCAGTT CGAAGCTGCC TATCCAGCTC CAAGAGGGAT TCCCAAAGGA
    AAGTTGGTCA AGTATATGAT GGGATTCCCA ATTATCATTG GAGTCGTTAT CTTCATCTTT
    TCTCCTCTAT TGCTCTGGTC GCTACTCAAT CAAATCGGAA CTATCAGTAT GCCAGAAAAA
    GTTACGTTGA GGATTTCGAT TGAAGGATAT CCACCACTTT ACGAGATGGA GGCACAAGGA
    AGTAATCACG ATAACGCTGA ACTTGGAATG ATAAAACCTG ATCAATTGGC ATCTTTGAAT
    CAAGCATTGA CGGATAGTTA CACGACACGA GACACTAATT CTATTTTAAG ATCAAGAATG
    TCTGTATCAT ATCTGAAAGG ATACACATAT GAGGATATTC TGATTGTGCG ATTCCGTCCA
    GAATCTGAAA TTTATTGGCC GATTTCACAG GATTCGAGAA ATGCGATGAT CGATAAATTG
    AGCCGAAACA CGTCAGTCAA TTTTGAAGTA TCTCTGGAAT TCACTCGGCC ATATGATCCA
    AATGAAAACG CTGCTTTAAA ACATTCGAAA TCGTGGCTTG TTCCAATCTC CCTGGATATG
    ACGATTCGAG CAAAAATTCA AAGTGCTCTC CGAGGAGATC CAGGTCATCC GATTCTCATT
    CCACAGTCAA TTCCTGCGTT TATTCAAGTT CCAAATCAAG GAGAATTGAC CCTTCCAACA
    TCAATCGGAA ATACTATTAT CAATGACGGA AATCCACGGA TTAATACGAC CGGGATGGAA
    AAATCAGACG AAGCTCGGGC TTGGTTCGAC TCGTTAACCT TGAATCTCGA ACAAGGAAAA
    TCGCAAAACG AGAAAATGTG GATCGCCACA TCCGAACATC CAGGAGACCA AAATGCGAAA
    CTCTGGATAA AGACTGCAAA TACGACGTAT TCGGGAAGGC CGTACCTTCA AGTTGTCGGA
    TTTATTGATA GAGCATTCCC ATCATTTTTG GCGAAAGTTT TCAAAGGAGG AGTCATTGCA
    GTGTATCTTT CAGTTATCCT CGTCGTTGGA AGAGGTCTGG TTCGTGGAAT CTTCACCACA
    TCTCCAAGCA CTGTCATGTT CACAGAATTA CCGAATGCGG ATCATCTTCT CAAAATTTGT
    CTCGACATCT ATTTGGTTCG AGAAGCGAAG GACTTCATGT TGGAGCAGGA TCTCTTCGCC
    AAATTAATAT TCCTGTTCCG ATCACCAGCG ACACTCATCG AATGGACACG AATGAGCAAA
    AAGAAGCAAG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa356223
    Clone ID Related Accession (Same CDS sequence) NM_001268457.3 , NM_001268457.2
    Accession Version NM_001268457.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5193bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268457.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTAACAA AGTCAATAGT GAGTAGTAGT GGTGACTCTG GTATTTACGA AGACGGTTGT 
    GGTATCATGC CGTACATTGA CGACGACGAT GATTTGACGC CAATTATAAT GGCAAGACCT
    GCTATTGGCA GTGGGTACAC GGGTGGCAGG AACGGATGGC ATAGATCACC CAGGCAGTAT
    TTGACCAACT GGTGGAGCGA TACCCTTGTC AAGAAGCTGC ACAAATTGGC AAATCAGACA
    ATCGAACTGT TATGGAGGTT TTTCGAAGTT CACATTTCGA AAATTGTCTT TGTCATCATT
    GCGATTTTTA TTGCCAACAA TATCAATGCT CTATACATTC CACTTGTTAT TCTCCTCTCA
    TTGGCTATAT GTCTACCATC AGCTGCAGAT GGGATATTCT CTTTATTTAT GTGTGCCTAC
    TTATTCCTCG TGGCACTGAG CAAGATGATC TACCAACTGG ATATTGTTCC CGAGTTATCA
    CAAATCGACC GAGGAGTTGG AGCAGATAAC TGTTCACATG GAAATATTTC AATGCCTGAA
    TGGTTTGGAT TGAAGAAGGA AGTTGAAGGA ACTGAACCAA TTTATATGCT CTTTGGAGTC
    ATTGTTTCGA TTATTGCATT GGCTTTTCAA TCAATTGTTA TATATCGACA AAGACATTAT
    CGAGCTTCAC TGGGACTTCC TGAATCGATG CGAGCTAAAG TGTTCCCGGA CTTCCATCAT
    TCTCATTTTG ATAGATCTCT CAAAAATGCC ATCCAATTCT TGATAGACTA TGGTTTCTAT
    AAGTTTGGAC TTGAAATCAC GATGATCGCC ATTGGAATTG ACATTTTCAA TCGAATGGAT
    GCATTGGCAG CGATTCAATG TTTCTGGCTT GTCCTGTTTG CGCTCAATAA AAGAGTCTTT
    GTTCGAAGAA TATGGGTATT CTATGTGATC TATATGGCGA TTCTGTATCC ACTTCAGTTC
    TTTTCATATG TTGGATTACC ACCTGACAGT TGTATTGAGT ATCCATGGTC GTATTGGATT
    CCATCGTATA GTGATGATGC GAGATTCAAT CTGTCGTATC TTCTGAACTT GTCAATATAC
    GGTGTAAACT GGCCATCAGC ATATCTCATT GGCGATTTCT TCGTACTCCT CTTGGCATCA
    TGCCAATTAG CAGTTTTCCG GCGAGAAGGA GAAGATAATG ATTCAATTTA CAATGATGGA
    AACTTTGTGA TTAAGCCAGA AAATCCACAA TATGACTTTA TTGACACCAA AAAGAGTTAC
    GTTGATTATT TCAAATCATT CGTTTTCCAT TATGGACACT GGATTACATT GATGTCTACA
    CTAGCAGCAG GAATTGCTGG AACGTCACTT TTTGCTTTGG GATACATTAT ATTCACTCTG
    ACAATGCTGT GGTCTGGTAA CAATTTGTAT GTGATGAACT CAACTCTACG ATCATTTGAA
    CATACTTTGA AACGATGGAA TGCACTTTTG GGATACACAT TGTTCACGAT TACAATGAAA
    GTTTGCCTTC AAATATTTGG ATGTGTATTC CTGTCGTGGT TCGATCAATC TGGTGGATGG
    GGAAAGACTC TGTGCATCGT TCGTCAACTC TTCAGTATCA CATGTGTTAA TAACGAATGT
    CATGTGCTCA AAGAGCTGGA GGATTTCTCG AAGGCGTGTG CTGTTGAGAC GAAAGAAGGA
    AATATTGGAT TTGATGTGAT TGCGCTATCC TTCCTCGTCT TCCAAATCCG AATTTTCCAT
    TCTTGGTATT TCCAACACTG CATGGTTGAA TATCGAAGTG AGGTTATTCT GGCCAATAGA
    GGTGCCGTGC TGAAGAATCA ACTGATTGAA AAGGAAATGA AGGAACAAAA CGAGCAGCAA
    AAAGCCAAGT TCAATGATAT CAGAAGAAGA ACTGAAGCTA TTCGTGAAAG ATATCAAAAA
    CAAATCGAAC GAGGAGCTGC TGAACGTGAC TTCGAGCCGG TTACTTATGG GCACGAATGT
    ATGCGTATCG ACGAGGATGA CCCATTTCCT TACTATGACT TGCGTATATC ATCGCAAGAT
    ACTGAAAATG AGGCCAAACG AGCGGGAGAC TACTACATGT TCAAATACGA TCCTGAAAAT
    GATGATCTTG TGGAACCCGT CGACAGTTTT GTACCAGAAG TTGATCCCAA GGCAACTGCA
    TATGATCGAC TTGATCCTGG ACAGATAATG TACGCTGCCA CCGCTCACGA CCTCGACCTC
    GCCAAAACTG TACAGCAAGT CAAGAAAGGC GATACTATCA AAGATCCTGA TTCACGTGCT
    CTTATCGCTG TTTCTGAACC AGAAGCTCGT AAGCCAGGAG GCACTGAAGA AACGGATGGT
    GATGAAGATG AGGATAATAA GGATTCGAAA GTGGAAAGTA CTGCTAAATT CATTCAAAAA
    ATGATCGCTT CTGCCTTGGA CTTGTGTAGT GTAACACTAA ACAAACTGTG CAGAGAACAC
    CGATATGTTG GATTTGTTTT GAGCAAGGAA AAACAAAAGT TGAAGTCAGG ACACTCGGAA
    TCTCTTTCAA ATACATCTAG AAAGCTGACG GATATTCGAT CTGCAGTTGA TCTACCAAGT
    CTTCAACTTG TGCAATCGGC GAATGATGTT GAAAAGATGG AGACAGCCGT CAGCGTGGAT
    TGGCAACAAA AATCATCAGC AACGAGACTA TTGAATGCAG TTGTGAATTG TATTGGAGCA
    CACACTGATA TTCTCTGCTA CTTCTTCGCA ATTATGACAC AAGTGATGAC TGGTGGACTT
    ATTACTCTTC CACTTCCTCT GATGTCACTA TTCTGGGGAA ATCTATCGAA TCCTCGTCCA
    TCCAAGTTCT TCTGGGTCAC AATGATCACC TACACTGAGT GTGTCATTGT TATCAAGTTT
    GTTTGCCAAT TTGCATTCAT GCCTTACAAT TCTATCACGT GGCGAACAGA GCATCAAATG
    GACCCGATGT CATTGGATAA GTTATTCGGA GTATCACAGA GAGATAGTTT TGCACTTTGG
    GACATTGTTT TGCTCTTCTC ACTCTTCTTC CATCGGTATA TGCTCAGAAA GTTAGGGTTA
    TGGAAGGATG CCAATTTGAC GGACACGTTC ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA
    GGATCGGACA CAGGATCACC CAAGAAAATT GCTCAAGAGC CAAAGGTTGT TGTAACTCAG
    TCAGATACCC TTGAAGGTAC AAGTGGAGGA GAGATAGTCA TACCATCTGA TCCAAATGCA
    GTTTCCAATA TGGAAGAATT AGACTGCGAA CCTCCGATTC CAGAGAAGCA AAGTGGACCA
    ATTGGAAGAT TCATTCATCA ATTATTCCAT CCAAAATTCC GTTACATTCG TGATTTGTAT
    CCAATAATGT TTGGAATCGA TGTGATATGC TTCCTCATCA TGACATTTGG ATACTCGGCA
    TTTGGTGAAG GAGGTTCTGG AAATGTTTTA GATGATGTGA AGGCTTCTCG TATTCCGGTA
    ACACTTGTTG TTATGCTCGT TGGAATGACT CTGGCAATTA TCATTGATCG AGCACTCTAC
    CTCAGAAAAT CAGTTGTCGG AAAGCTAATC TATCAAGTCT TAATGATTGC TTTCCTTCAC
    ATTTGGGTAT TCCTTGTTCT TCCAAATATG ACTCGTCGTT CTGCGATATC GAATCACGTT
    GCTCAAGCAT TGTACGTCAT CAAATCGTGC TATTTCCTTG TGTCTGCTTG GCAGATCCGA
    AATGGTTATC CTGAACTTTG TATTGGAAAT CTATTAACAC ATTCATACGG AATGACCAAT
    ATGATTGCAT TCAAAGTATT CATGAATATT CCATTCCTCT TCGAGCTTCG TACAGCTATT
    GATTGGACAT GGACAGATAC TTCAATGCCT CTTTTCGATT TCTTCAATAT GGAAAATTTC
    TATGCTCATA TATTCAATAT CAAGTGTGCC CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA
    AGAGGGATTC CCAAAGGAAA GTTGGTCAAG TATATGATGG GATTCCCAAT TATCATTGGA
    GTCGTTATCT TCATCTTTTC TCCTCTATTG CTCTGGTCGC TACTCAATCA AATCGGAACT
    ATCAGTATGC CAGAAAAAGT TACGTTGAGG ATTTCGATTG AAGGATATCC ACCACTTTAC
    GAGATGGAGG CACAAGGAAG TAATCACGAT AACGCTGAAC TTGGAATGAT AAAACCTGAT
    CAATTGGCAT CTTTGAATCA AGCATTGACG GATAGTTACA CGACACGAGA CACTAATTCT
    ATTTTAAGAT CAAGAATGTC TGTATCATAT CTGAAAGGAT ACACATATGA GGATATTCTG
    ATTGTGCGAT TCCGTCCAGA ATCTGAAATT TATTGGCCGA TTTCACAGGA TTCGAGAAAT
    GCGATGATCG ATAAATTGAG CCGAAACACG TCAGTCAATT TTGAAGTATC TCTGGAATTC
    ACTCGGCCAT ATGATCCAAA TGAAAACGCT GCTTTAAAAC ATTCGAAATC GTGGCTTGTT
    CCAATCTCCC TGGATATGAC GATTCGAGCA AAAATTCAAA GTGCTCTCCG AGGAGATCCA
    GGTCATCCGA TTCTCATTCC ACAGTCAATT CCTGCGTTTA TTCAAGTTCC AAATCAAGGA
    GAATTGACCC TTCCAACATC AATCGGAAAT ACTATTATCA ATGACGGAAA TCCACGGATT
    AATACGACCG GGATGGAAAA ATCAGACGAA GCTCGGGCTT GGTTCGACTC GTTAACCTTG
    AATCTCGAAC AAGGAAAATC GCAAAACGAG AAAATGTGGA TCGCCACATC CGAACATCCA
    GGAGACCAAA ATGCGAAACT CTGGATAAAG ACTGCAAATA CGACGTATTC GGGAAGGCCG
    TACCTTCAAG TTGTCGGATT TATTGATAGA GCATTCCCAT CATTTTTGGC GAAAGTTTTC
    AAAGGAGGAG TCATTGCAGT GTATCTTTCA GTTATCCTCG TCGTTGGAAG AGGTCTGGTT
    CGTGGAATCT TCACCACATC TCCAAGCACT GTCATGTTCA CAGAATTACC GAATGCGGAT
    CATCTTCTCA AAATTTGTCT CGACATCTAT TTGGTTCGAG AAGCGAAGGA CTTCATGTTG
    GAGCAGGATC TCTTCGCCAA ATTAATATTC CTGTTCCGAT CACCAGCGAC ACTCATCGAA
    TGGACACGAA TGAGCAAAAA GAAGCAAGAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255386.2
    CDS271..5463
    Translation

    Target ORF information:

    RefSeq Version NM_001268457.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268457.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTAACAA AGTCAATAGT GAGTAGTAGT GGTGACTCTG GTATTTACGA AGACGGTTGT 
    GGTATCATGC CGTACATTGA CGACGACGAT GATTTGACGC CAATTATAAT GGCAAGACCT
    GCTATTGGCA GTGGGTACAC GGGTGGCAGG AACGGATGGC ATAGATCACC CAGGCAGTAT
    TTGACCAACT GGTGGAGCGA TACCCTTGTC AAGAAGCTGC ACAAATTGGC AAATCAGACA
    ATCGAACTGT TATGGAGGTT TTTCGAAGTT CACATTTCGA AAATTGTCTT TGTCATCATT
    GCGATTTTTA TTGCCAACAA TATCAATGCT CTATACATTC CACTTGTTAT TCTCCTCTCA
    TTGGCTATAT GTCTACCATC AGCTGCAGAT GGGATATTCT CTTTATTTAT GTGTGCCTAC
    TTATTCCTCG TGGCACTGAG CAAGATGATC TACCAACTGG ATATTGTTCC CGAGTTATCA
    CAAATCGACC GAGGAGTTGG AGCAGATAAC TGTTCACATG GAAATATTTC AATGCCTGAA
    TGGTTTGGAT TGAAGAAGGA AGTTGAAGGA ACTGAACCAA TTTATATGCT CTTTGGAGTC
    ATTGTTTCGA TTATTGCATT GGCTTTTCAA TCAATTGTTA TATATCGACA AAGACATTAT
    CGAGCTTCAC TGGGACTTCC TGAATCGATG CGAGCTAAAG TGTTCCCGGA CTTCCATCAT
    TCTCATTTTG ATAGATCTCT CAAAAATGCC ATCCAATTCT TGATAGACTA TGGTTTCTAT
    AAGTTTGGAC TTGAAATCAC GATGATCGCC ATTGGAATTG ACATTTTCAA TCGAATGGAT
    GCATTGGCAG CGATTCAATG TTTCTGGCTT GTCCTGTTTG CGCTCAATAA AAGAGTCTTT
    GTTCGAAGAA TATGGGTATT CTATGTGATC TATATGGCGA TTCTGTATCC ACTTCAGTTC
    TTTTCATATG TTGGATTACC ACCTGACAGT TGTATTGAGT ATCCATGGTC GTATTGGATT
    CCATCGTATA GTGATGATGC GAGATTCAAT CTGTCGTATC TTCTGAACTT GTCAATATAC
    GGTGTAAACT GGCCATCAGC ATATCTCATT GGCGATTTCT TCGTACTCCT CTTGGCATCA
    TGCCAATTAG CAGTTTTCCG GCGAGAAGGA GAAGATAATG ATTCAATTTA CAATGATGGA
    AACTTTGTGA TTAAGCCAGA AAATCCACAA TATGACTTTA TTGACACCAA AAAGAGTTAC
    GTTGATTATT TCAAATCATT CGTTTTCCAT TATGGACACT GGATTACATT GATGTCTACA
    CTAGCAGCAG GAATTGCTGG AACGTCACTT TTTGCTTTGG GATACATTAT ATTCACTCTG
    ACAATGCTGT GGTCTGGTAA CAATTTGTAT GTGATGAACT CAACTCTACG ATCATTTGAA
    CATACTTTGA AACGATGGAA TGCACTTTTG GGATACACAT TGTTCACGAT TACAATGAAA
    GTTTGCCTTC AAATATTTGG ATGTGTATTC CTGTCGTGGT TCGATCAATC TGGTGGATGG
    GGAAAGACTC TGTGCATCGT TCGTCAACTC TTCAGTATCA CATGTGTTAA TAACGAATGT
    CATGTGCTCA AAGAGCTGGA GGATTTCTCG AAGGCGTGTG CTGTTGAGAC GAAAGAAGGA
    AATATTGGAT TTGATGTGAT TGCGCTATCC TTCCTCGTCT TCCAAATCCG AATTTTCCAT
    TCTTGGTATT TCCAACACTG CATGGTTGAA TATCGAAGTG AGGTTATTCT GGCCAATAGA
    GGTGCCGTGC TGAAGAATCA ACTGATTGAA AAGGAAATGA AGGAACAAAA CGAGCAGCAA
    AAAGCCAAGT TCAATGATAT CAGAAGAAGA ACTGAAGCTA TTCGTGAAAG ATATCAAAAA
    CAAATCGAAC GAGGAGCTGC TGAACGTGAC TTCGAGCCGG TTACTTATGG GCACGAATGT
    ATGCGTATCG ACGAGGATGA CCCATTTCCT TACTATGACT TGCGTATATC ATCGCAAGAT
    ACTGAAAATG AGGCCAAACG AGCGGGAGAC TACTACATGT TCAAATACGA TCCTGAAAAT
    GATGATCTTG TGGAACCCGT CGACAGTTTT GTACCAGAAG TTGATCCCAA GGCAACTGCA
    TATGATCGAC TTGATCCTGG ACAGATAATG TACGCTGCCA CCGCTCACGA CCTCGACCTC
    GCCAAAACTG TACAGCAAGT CAAGAAAGGC GATACTATCA AAGATCCTGA TTCACGTGCT
    CTTATCGCTG TTTCTGAACC AGAAGCTCGT AAGCCAGGAG GCACTGAAGA AACGGATGGT
    GATGAAGATG AGGATAATAA GGATTCGAAA GTGGAAAGTA CTGCTAAATT CATTCAAAAA
    ATGATCGCTT CTGCCTTGGA CTTGTGTAGT GTAACACTAA ACAAACTGTG CAGAGAACAC
    CGATATGTTG GATTTGTTTT GAGCAAGGAA AAACAAAAGT TGAAGTCAGG ACACTCGGAA
    TCTCTTTCAA ATACATCTAG AAAGCTGACG GATATTCGAT CTGCAGTTGA TCTACCAAGT
    CTTCAACTTG TGCAATCGGC GAATGATGTT GAAAAGATGG AGACAGCCGT CAGCGTGGAT
    TGGCAACAAA AATCATCAGC AACGAGACTA TTGAATGCAG TTGTGAATTG TATTGGAGCA
    CACACTGATA TTCTCTGCTA CTTCTTCGCA ATTATGACAC AAGTGATGAC TGGTGGACTT
    ATTACTCTTC CACTTCCTCT GATGTCACTA TTCTGGGGAA ATCTATCGAA TCCTCGTCCA
    TCCAAGTTCT TCTGGGTCAC AATGATCACC TACACTGAGT GTGTCATTGT TATCAAGTTT
    GTTTGCCAAT TTGCATTCAT GCCTTACAAT TCTATCACGT GGCGAACAGA GCATCAAATG
    GACCCGATGT CATTGGATAA GTTATTCGGA GTATCACAGA GAGATAGTTT TGCACTTTGG
    GACATTGTTT TGCTCTTCTC ACTCTTCTTC CATCGGTATA TGCTCAGAAA GTTAGGGTTA
    TGGAAGGATG CCAATTTGAC GGACACGTTC ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA
    GGATCGGACA CAGGATCACC CAAGAAAATT GCTCAAGAGC CAAAGGTTGT TGTAACTCAG
    TCAGATACCC TTGAAGGTAC AAGTGGAGGA GAGATAGTCA TACCATCTGA TCCAAATGCA
    GTTTCCAATA TGGAAGAATT AGACTGCGAA CCTCCGATTC CAGAGAAGCA AAGTGGACCA
    ATTGGAAGAT TCATTCATCA ATTATTCCAT CCAAAATTCC GTTACATTCG TGATTTGTAT
    CCAATAATGT TTGGAATCGA TGTGATATGC TTCCTCATCA TGACATTTGG ATACTCGGCA
    TTTGGTGAAG GAGGTTCTGG AAATGTTTTA GATGATGTGA AGGCTTCTCG TATTCCGGTA
    ACACTTGTTG TTATGCTCGT TGGAATGACT CTGGCAATTA TCATTGATCG AGCACTCTAC
    CTCAGAAAAT CAGTTGTCGG AAAGCTAATC TATCAAGTCT TAATGATTGC TTTCCTTCAC
    ATTTGGGTAT TCCTTGTTCT TCCAAATATG ACTCGTCGTT CTGCGATATC GAATCACGTT
    GCTCAAGCAT TGTACGTCAT CAAATCGTGC TATTTCCTTG TGTCTGCTTG GCAGATCCGA
    AATGGTTATC CTGAACTTTG TATTGGAAAT CTATTAACAC ATTCATACGG AATGACCAAT
    ATGATTGCAT TCAAAGTATT CATGAATATT CCATTCCTCT TCGAGCTTCG TACAGCTATT
    GATTGGACAT GGACAGATAC TTCAATGCCT CTTTTCGATT TCTTCAATAT GGAAAATTTC
    TATGCTCATA TATTCAATAT CAAGTGTGCC CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA
    AGAGGGATTC CCAAAGGAAA GTTGGTCAAG TATATGATGG GATTCCCAAT TATCATTGGA
    GTCGTTATCT TCATCTTTTC TCCTCTATTG CTCTGGTCGC TACTCAATCA AATCGGAACT
    ATCAGTATGC CAGAAAAAGT TACGTTGAGG ATTTCGATTG AAGGATATCC ACCACTTTAC
    GAGATGGAGG CACAAGGAAG TAATCACGAT AACGCTGAAC TTGGAATGAT AAAACCTGAT
    CAATTGGCAT CTTTGAATCA AGCATTGACG GATAGTTACA CGACACGAGA CACTAATTCT
    ATTTTAAGAT CAAGAATGTC TGTATCATAT CTGAAAGGAT ACACATATGA GGATATTCTG
    ATTGTGCGAT TCCGTCCAGA ATCTGAAATT TATTGGCCGA TTTCACAGGA TTCGAGAAAT
    GCGATGATCG ATAAATTGAG CCGAAACACG TCAGTCAATT TTGAAGTATC TCTGGAATTC
    ACTCGGCCAT ATGATCCAAA TGAAAACGCT GCTTTAAAAC ATTCGAAATC GTGGCTTGTT
    CCAATCTCCC TGGATATGAC GATTCGAGCA AAAATTCAAA GTGCTCTCCG AGGAGATCCA
    GGTCATCCGA TTCTCATTCC ACAGTCAATT CCTGCGTTTA TTCAAGTTCC AAATCAAGGA
    GAATTGACCC TTCCAACATC AATCGGAAAT ACTATTATCA ATGACGGAAA TCCACGGATT
    AATACGACCG GGATGGAAAA ATCAGACGAA GCTCGGGCTT GGTTCGACTC GTTAACCTTG
    AATCTCGAAC AAGGAAAATC GCAAAACGAG AAAATGTGGA TCGCCACATC CGAACATCCA
    GGAGACCAAA ATGCGAAACT CTGGATAAAG ACTGCAAATA CGACGTATTC GGGAAGGCCG
    TACCTTCAAG TTGTCGGATT TATTGATAGA GCATTCCCAT CATTTTTGGC GAAAGTTTTC
    AAAGGAGGAG TCATTGCAGT GTATCTTTCA GTTATCCTCG TCGTTGGAAG AGGTCTGGTT
    CGTGGAATCT TCACCACATC TCCAAGCACT GTCATGTTCA CAGAATTACC GAATGCGGAT
    CATCTTCTCA AAATTTGTCT CGACATCTAT TTGGTTCGAG AAGCGAAGGA CTTCATGTTG
    GAGCAGGATC TCTTCGCCAA ATTAATATTC CTGTTCCGAT CACCAGCGAC ACTCATCGAA
    TGGACACGAA TGAGCAAAAA GAAGCAAGAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa356223
    Clone ID Related Accession (Same CDS sequence) NM_001268457.3 , NM_001268457.2
    Accession Version NM_001268457.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5193bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Apr 20, 2015 this sequence version replaced NM_001268457.1. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTAACAA AGTCAATAGT GAGTAGTAGT GGTGACTCTG GTATTTACGA AGACGGTTGT 
    GGTATCATGC CGTACATTGA CGACGACGAT GATTTGACGC CAATTATAAT GGCAAGACCT
    GCTATTGGCA GTGGGTACAC GGGTGGCAGG AACGGATGGC ATAGATCACC CAGGCAGTAT
    TTGACCAACT GGTGGAGCGA TACCCTTGTC AAGAAGCTGC ACAAATTGGC AAATCAGACA
    ATCGAACTGT TATGGAGGTT TTTCGAAGTT CACATTTCGA AAATTGTCTT TGTCATCATT
    GCGATTTTTA TTGCCAACAA TATCAATGCT CTATACATTC CACTTGTTAT TCTCCTCTCA
    TTGGCTATAT GTCTACCATC AGCTGCAGAT GGGATATTCT CTTTATTTAT GTGTGCCTAC
    TTATTCCTCG TGGCACTGAG CAAGATGATC TACCAACTGG ATATTGTTCC CGAGTTATCA
    CAAATCGACC GAGGAGTTGG AGCAGATAAC TGTTCACATG GAAATATTTC AATGCCTGAA
    TGGTTTGGAT TGAAGAAGGA AGTTGAAGGA ACTGAACCAA TTTATATGCT CTTTGGAGTC
    ATTGTTTCGA TTATTGCATT GGCTTTTCAA TCAATTGTTA TATATCGACA AAGACATTAT
    CGAGCTTCAC TGGGACTTCC TGAATCGATG CGAGCTAAAG TGTTCCCGGA CTTCCATCAT
    TCTCATTTTG ATAGATCTCT CAAAAATGCC ATCCAATTCT TGATAGACTA TGGTTTCTAT
    AAGTTTGGAC TTGAAATCAC GATGATCGCC ATTGGAATTG ACATTTTCAA TCGAATGGAT
    GCATTGGCAG CGATTCAATG TTTCTGGCTT GTCCTGTTTG CGCTCAATAA AAGAGTCTTT
    GTTCGAAGAA TATGGGTATT CTATGTGATC TATATGGCGA TTCTGTATCC ACTTCAGTTC
    TTTTCATATG TTGGATTACC ACCTGACAGT TGTATTGAGT ATCCATGGTC GTATTGGATT
    CCATCGTATA GTGATGATGC GAGATTCAAT CTGTCGTATC TTCTGAACTT GTCAATATAC
    GGTGTAAACT GGCCATCAGC ATATCTCATT GGCGATTTCT TCGTACTCCT CTTGGCATCA
    TGCCAATTAG CAGTTTTCCG GCGAGAAGGA GAAGATAATG ATTCAATTTA CAATGATGGA
    AACTTTGTGA TTAAGCCAGA AAATCCACAA TATGACTTTA TTGACACCAA AAAGAGTTAC
    GTTGATTATT TCAAATCATT CGTTTTCCAT TATGGACACT GGATTACATT GATGTCTACA
    CTAGCAGCAG GAATTGCTGG AACGTCACTT TTTGCTTTGG GATACATTAT ATTCACTCTG
    ACAATGCTGT GGTCTGGTAA CAATTTGTAT GTGATGAACT CAACTCTACG ATCATTTGAA
    CATACTTTGA AACGATGGAA TGCACTTTTG GGATACACAT TGTTCACGAT TACAATGAAA
    GTTTGCCTTC AAATATTTGG ATGTGTATTC CTGTCGTGGT TCGATCAATC TGGTGGATGG
    GGAAAGACTC TGTGCATCGT TCGTCAACTC TTCAGTATCA CATGTGTTAA TAACGAATGT
    CATGTGCTCA AAGAGCTGGA GGATTTCTCG AAGGCGTGTG CTGTTGAGAC GAAAGAAGGA
    AATATTGGAT TTGATGTGAT TGCGCTATCC TTCCTCGTCT TCCAAATCCG AATTTTCCAT
    TCTTGGTATT TCCAACACTG CATGGTTGAA TATCGAAGTG AGGTTATTCT GGCCAATAGA
    GGTGCCGTGC TGAAGAATCA ACTGATTGAA AAGGAAATGA AGGAACAAAA CGAGCAGCAA
    AAAGCCAAGT TCAATGATAT CAGAAGAAGA ACTGAAGCTA TTCGTGAAAG ATATCAAAAA
    CAAATCGAAC GAGGAGCTGC TGAACGTGAC TTCGAGCCGG TTACTTATGG GCACGAATGT
    ATGCGTATCG ACGAGGATGA CCCATTTCCT TACTATGACT TGCGTATATC ATCGCAAGAT
    ACTGAAAATG AGGCCAAACG AGCGGGAGAC TACTACATGT TCAAATACGA TCCTGAAAAT
    GATGATCTTG TGGAACCCGT CGACAGTTTT GTACCAGAAG TTGATCCCAA GGCAACTGCA
    TATGATCGAC TTGATCCTGG ACAGATAATG TACGCTGCCA CCGCTCACGA CCTCGACCTC
    GCCAAAACTG TACAGCAAGT CAAGAAAGGC GATACTATCA AAGATCCTGA TTCACGTGCT
    CTTATCGCTG TTTCTGAACC AGAAGCTCGT AAGCCAGGAG GCACTGAAGA AACGGATGGT
    GATGAAGATG AGGATAATAA GGATTCGAAA GTGGAAAGTA CTGCTAAATT CATTCAAAAA
    ATGATCGCTT CTGCCTTGGA CTTGTGTAGT GTAACACTAA ACAAACTGTG CAGAGAACAC
    CGATATGTTG GATTTGTTTT GAGCAAGGAA AAACAAAAGT TGAAGTCAGG ACACTCGGAA
    TCTCTTTCAA ATACATCTAG AAAGCTGACG GATATTCGAT CTGCAGTTGA TCTACCAAGT
    CTTCAACTTG TGCAATCGGC GAATGATGTT GAAAAGATGG AGACAGCCGT CAGCGTGGAT
    TGGCAACAAA AATCATCAGC AACGAGACTA TTGAATGCAG TTGTGAATTG TATTGGAGCA
    CACACTGATA TTCTCTGCTA CTTCTTCGCA ATTATGACAC AAGTGATGAC TGGTGGACTT
    ATTACTCTTC CACTTCCTCT GATGTCACTA TTCTGGGGAA ATCTATCGAA TCCTCGTCCA
    TCCAAGTTCT TCTGGGTCAC AATGATCACC TACACTGAGT GTGTCATTGT TATCAAGTTT
    GTTTGCCAAT TTGCATTCAT GCCTTACAAT TCTATCACGT GGCGAACAGA GCATCAAATG
    GACCCGATGT CATTGGATAA GTTATTCGGA GTATCACAGA GAGATAGTTT TGCACTTTGG
    GACATTGTTT TGCTCTTCTC ACTCTTCTTC CATCGGTATA TGCTCAGAAA GTTAGGGTTA
    TGGAAGGATG CCAATTTGAC GGACACGTTC ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA
    GGATCGGACA CAGGATCACC CAAGAAAATT GCTCAAGAGC CAAAGGTTGT TGTAACTCAG
    TCAGATACCC TTGAAGGTAC AAGTGGAGGA GAGATAGTCA TACCATCTGA TCCAAATGCA
    GTTTCCAATA TGGAAGAATT AGACTGCGAA CCTCCGATTC CAGAGAAGCA AAGTGGACCA
    ATTGGAAGAT TCATTCATCA ATTATTCCAT CCAAAATTCC GTTACATTCG TGATTTGTAT
    CCAATAATGT TTGGAATCGA TGTGATATGC TTCCTCATCA TGACATTTGG ATACTCGGCA
    TTTGGTGAAG GAGGTTCTGG AAATGTTTTA GATGATGTGA AGGCTTCTCG TATTCCGGTA
    ACACTTGTTG TTATGCTCGT TGGAATGACT CTGGCAATTA TCATTGATCG AGCACTCTAC
    CTCAGAAAAT CAGTTGTCGG AAAGCTAATC TATCAAGTCT TAATGATTGC TTTCCTTCAC
    ATTTGGGTAT TCCTTGTTCT TCCAAATATG ACTCGTCGTT CTGCGATATC GAATCACGTT
    GCTCAAGCAT TGTACGTCAT CAAATCGTGC TATTTCCTTG TGTCTGCTTG GCAGATCCGA
    AATGGTTATC CTGAACTTTG TATTGGAAAT CTATTAACAC ATTCATACGG AATGACCAAT
    ATGATTGCAT TCAAAGTATT CATGAATATT CCATTCCTCT TCGAGCTTCG TACAGCTATT
    GATTGGACAT GGACAGATAC TTCAATGCCT CTTTTCGATT TCTTCAATAT GGAAAATTTC
    TATGCTCATA TATTCAATAT CAAGTGTGCC CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA
    AGAGGGATTC CCAAAGGAAA GTTGGTCAAG TATATGATGG GATTCCCAAT TATCATTGGA
    GTCGTTATCT TCATCTTTTC TCCTCTATTG CTCTGGTCGC TACTCAATCA AATCGGAACT
    ATCAGTATGC CAGAAAAAGT TACGTTGAGG ATTTCGATTG AAGGATATCC ACCACTTTAC
    GAGATGGAGG CACAAGGAAG TAATCACGAT AACGCTGAAC TTGGAATGAT AAAACCTGAT
    CAATTGGCAT CTTTGAATCA AGCATTGACG GATAGTTACA CGACACGAGA CACTAATTCT
    ATTTTAAGAT CAAGAATGTC TGTATCATAT CTGAAAGGAT ACACATATGA GGATATTCTG
    ATTGTGCGAT TCCGTCCAGA ATCTGAAATT TATTGGCCGA TTTCACAGGA TTCGAGAAAT
    GCGATGATCG ATAAATTGAG CCGAAACACG TCAGTCAATT TTGAAGTATC TCTGGAATTC
    ACTCGGCCAT ATGATCCAAA TGAAAACGCT GCTTTAAAAC ATTCGAAATC GTGGCTTGTT
    CCAATCTCCC TGGATATGAC GATTCGAGCA AAAATTCAAA GTGCTCTCCG AGGAGATCCA
    GGTCATCCGA TTCTCATTCC ACAGTCAATT CCTGCGTTTA TTCAAGTTCC AAATCAAGGA
    GAATTGACCC TTCCAACATC AATCGGAAAT ACTATTATCA ATGACGGAAA TCCACGGATT
    AATACGACCG GGATGGAAAA ATCAGACGAA GCTCGGGCTT GGTTCGACTC GTTAACCTTG
    AATCTCGAAC AAGGAAAATC GCAAAACGAG AAAATGTGGA TCGCCACATC CGAACATCCA
    GGAGACCAAA ATGCGAAACT CTGGATAAAG ACTGCAAATA CGACGTATTC GGGAAGGCCG
    TACCTTCAAG TTGTCGGATT TATTGATAGA GCATTCCCAT CATTTTTGGC GAAAGTTTTC
    AAAGGAGGAG TCATTGCAGT GTATCTTTCA GTTATCCTCG TCGTTGGAAG AGGTCTGGTT
    CGTGGAATCT TCACCACATC TCCAAGCACT GTCATGTTCA CAGAATTACC GAATGCGGAT
    CATCTTCTCA AAATTTGTCT CGACATCTAT TTGGTTCGAG AAGCGAAGGA CTTCATGTTG
    GAGCAGGATC TCTTCGCCAA ATTAATATTC CTGTTCCGAT CACCAGCGAC ACTCATCGAA
    TGGACACGAA TGAGCAAAAA GAAGCAAGAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255386.2
    CDS1..5193
    Translation

    Target ORF information:

    RefSeq Version NM_001268457.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268457.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTAACAA AGTCAATAGT GAGTAGTAGT GGTGACTCTG GTATTTACGA AGACGGTTGT 
    GGTATCATGC CGTACATTGA CGACGACGAT GATTTGACGC CAATTATAAT GGCAAGACCT
    GCTATTGGCA GTGGGTACAC GGGTGGCAGG AACGGATGGC ATAGATCACC CAGGCAGTAT
    TTGACCAACT GGTGGAGCGA TACCCTTGTC AAGAAGCTGC ACAAATTGGC AAATCAGACA
    ATCGAACTGT TATGGAGGTT TTTCGAAGTT CACATTTCGA AAATTGTCTT TGTCATCATT
    GCGATTTTTA TTGCCAACAA TATCAATGCT CTATACATTC CACTTGTTAT TCTCCTCTCA
    TTGGCTATAT GTCTACCATC AGCTGCAGAT GGGATATTCT CTTTATTTAT GTGTGCCTAC
    TTATTCCTCG TGGCACTGAG CAAGATGATC TACCAACTGG ATATTGTTCC CGAGTTATCA
    CAAATCGACC GAGGAGTTGG AGCAGATAAC TGTTCACATG GAAATATTTC AATGCCTGAA
    TGGTTTGGAT TGAAGAAGGA AGTTGAAGGA ACTGAACCAA TTTATATGCT CTTTGGAGTC
    ATTGTTTCGA TTATTGCATT GGCTTTTCAA TCAATTGTTA TATATCGACA AAGACATTAT
    CGAGCTTCAC TGGGACTTCC TGAATCGATG CGAGCTAAAG TGTTCCCGGA CTTCCATCAT
    TCTCATTTTG ATAGATCTCT CAAAAATGCC ATCCAATTCT TGATAGACTA TGGTTTCTAT
    AAGTTTGGAC TTGAAATCAC GATGATCGCC ATTGGAATTG ACATTTTCAA TCGAATGGAT
    GCATTGGCAG CGATTCAATG TTTCTGGCTT GTCCTGTTTG CGCTCAATAA AAGAGTCTTT
    GTTCGAAGAA TATGGGTATT CTATGTGATC TATATGGCGA TTCTGTATCC ACTTCAGTTC
    TTTTCATATG TTGGATTACC ACCTGACAGT TGTATTGAGT ATCCATGGTC GTATTGGATT
    CCATCGTATA GTGATGATGC GAGATTCAAT CTGTCGTATC TTCTGAACTT GTCAATATAC
    GGTGTAAACT GGCCATCAGC ATATCTCATT GGCGATTTCT TCGTACTCCT CTTGGCATCA
    TGCCAATTAG CAGTTTTCCG GCGAGAAGGA GAAGATAATG ATTCAATTTA CAATGATGGA
    AACTTTGTGA TTAAGCCAGA AAATCCACAA TATGACTTTA TTGACACCAA AAAGAGTTAC
    GTTGATTATT TCAAATCATT CGTTTTCCAT TATGGACACT GGATTACATT GATGTCTACA
    CTAGCAGCAG GAATTGCTGG AACGTCACTT TTTGCTTTGG GATACATTAT ATTCACTCTG
    ACAATGCTGT GGTCTGGTAA CAATTTGTAT GTGATGAACT CAACTCTACG ATCATTTGAA
    CATACTTTGA AACGATGGAA TGCACTTTTG GGATACACAT TGTTCACGAT TACAATGAAA
    GTTTGCCTTC AAATATTTGG ATGTGTATTC CTGTCGTGGT TCGATCAATC TGGTGGATGG
    GGAAAGACTC TGTGCATCGT TCGTCAACTC TTCAGTATCA CATGTGTTAA TAACGAATGT
    CATGTGCTCA AAGAGCTGGA GGATTTCTCG AAGGCGTGTG CTGTTGAGAC GAAAGAAGGA
    AATATTGGAT TTGATGTGAT TGCGCTATCC TTCCTCGTCT TCCAAATCCG AATTTTCCAT
    TCTTGGTATT TCCAACACTG CATGGTTGAA TATCGAAGTG AGGTTATTCT GGCCAATAGA
    GGTGCCGTGC TGAAGAATCA ACTGATTGAA AAGGAAATGA AGGAACAAAA CGAGCAGCAA
    AAAGCCAAGT TCAATGATAT CAGAAGAAGA ACTGAAGCTA TTCGTGAAAG ATATCAAAAA
    CAAATCGAAC GAGGAGCTGC TGAACGTGAC TTCGAGCCGG TTACTTATGG GCACGAATGT
    ATGCGTATCG ACGAGGATGA CCCATTTCCT TACTATGACT TGCGTATATC ATCGCAAGAT
    ACTGAAAATG AGGCCAAACG AGCGGGAGAC TACTACATGT TCAAATACGA TCCTGAAAAT
    GATGATCTTG TGGAACCCGT CGACAGTTTT GTACCAGAAG TTGATCCCAA GGCAACTGCA
    TATGATCGAC TTGATCCTGG ACAGATAATG TACGCTGCCA CCGCTCACGA CCTCGACCTC
    GCCAAAACTG TACAGCAAGT CAAGAAAGGC GATACTATCA AAGATCCTGA TTCACGTGCT
    CTTATCGCTG TTTCTGAACC AGAAGCTCGT AAGCCAGGAG GCACTGAAGA AACGGATGGT
    GATGAAGATG AGGATAATAA GGATTCGAAA GTGGAAAGTA CTGCTAAATT CATTCAAAAA
    ATGATCGCTT CTGCCTTGGA CTTGTGTAGT GTAACACTAA ACAAACTGTG CAGAGAACAC
    CGATATGTTG GATTTGTTTT GAGCAAGGAA AAACAAAAGT TGAAGTCAGG ACACTCGGAA
    TCTCTTTCAA ATACATCTAG AAAGCTGACG GATATTCGAT CTGCAGTTGA TCTACCAAGT
    CTTCAACTTG TGCAATCGGC GAATGATGTT GAAAAGATGG AGACAGCCGT CAGCGTGGAT
    TGGCAACAAA AATCATCAGC AACGAGACTA TTGAATGCAG TTGTGAATTG TATTGGAGCA
    CACACTGATA TTCTCTGCTA CTTCTTCGCA ATTATGACAC AAGTGATGAC TGGTGGACTT
    ATTACTCTTC CACTTCCTCT GATGTCACTA TTCTGGGGAA ATCTATCGAA TCCTCGTCCA
    TCCAAGTTCT TCTGGGTCAC AATGATCACC TACACTGAGT GTGTCATTGT TATCAAGTTT
    GTTTGCCAAT TTGCATTCAT GCCTTACAAT TCTATCACGT GGCGAACAGA GCATCAAATG
    GACCCGATGT CATTGGATAA GTTATTCGGA GTATCACAGA GAGATAGTTT TGCACTTTGG
    GACATTGTTT TGCTCTTCTC ACTCTTCTTC CATCGGTATA TGCTCAGAAA GTTAGGGTTA
    TGGAAGGATG CCAATTTGAC GGACACGTTC ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA
    GGATCGGACA CAGGATCACC CAAGAAAATT GCTCAAGAGC CAAAGGTTGT TGTAACTCAG
    TCAGATACCC TTGAAGGTAC AAGTGGAGGA GAGATAGTCA TACCATCTGA TCCAAATGCA
    GTTTCCAATA TGGAAGAATT AGACTGCGAA CCTCCGATTC CAGAGAAGCA AAGTGGACCA
    ATTGGAAGAT TCATTCATCA ATTATTCCAT CCAAAATTCC GTTACATTCG TGATTTGTAT
    CCAATAATGT TTGGAATCGA TGTGATATGC TTCCTCATCA TGACATTTGG ATACTCGGCA
    TTTGGTGAAG GAGGTTCTGG AAATGTTTTA GATGATGTGA AGGCTTCTCG TATTCCGGTA
    ACACTTGTTG TTATGCTCGT TGGAATGACT CTGGCAATTA TCATTGATCG AGCACTCTAC
    CTCAGAAAAT CAGTTGTCGG AAAGCTAATC TATCAAGTCT TAATGATTGC TTTCCTTCAC
    ATTTGGGTAT TCCTTGTTCT TCCAAATATG ACTCGTCGTT CTGCGATATC GAATCACGTT
    GCTCAAGCAT TGTACGTCAT CAAATCGTGC TATTTCCTTG TGTCTGCTTG GCAGATCCGA
    AATGGTTATC CTGAACTTTG TATTGGAAAT CTATTAACAC ATTCATACGG AATGACCAAT
    ATGATTGCAT TCAAAGTATT CATGAATATT CCATTCCTCT TCGAGCTTCG TACAGCTATT
    GATTGGACAT GGACAGATAC TTCAATGCCT CTTTTCGATT TCTTCAATAT GGAAAATTTC
    TATGCTCATA TATTCAATAT CAAGTGTGCC CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA
    AGAGGGATTC CCAAAGGAAA GTTGGTCAAG TATATGATGG GATTCCCAAT TATCATTGGA
    GTCGTTATCT TCATCTTTTC TCCTCTATTG CTCTGGTCGC TACTCAATCA AATCGGAACT
    ATCAGTATGC CAGAAAAAGT TACGTTGAGG ATTTCGATTG AAGGATATCC ACCACTTTAC
    GAGATGGAGG CACAAGGAAG TAATCACGAT AACGCTGAAC TTGGAATGAT AAAACCTGAT
    CAATTGGCAT CTTTGAATCA AGCATTGACG GATAGTTACA CGACACGAGA CACTAATTCT
    ATTTTAAGAT CAAGAATGTC TGTATCATAT CTGAAAGGAT ACACATATGA GGATATTCTG
    ATTGTGCGAT TCCGTCCAGA ATCTGAAATT TATTGGCCGA TTTCACAGGA TTCGAGAAAT
    GCGATGATCG ATAAATTGAG CCGAAACACG TCAGTCAATT TTGAAGTATC TCTGGAATTC
    ACTCGGCCAT ATGATCCAAA TGAAAACGCT GCTTTAAAAC ATTCGAAATC GTGGCTTGTT
    CCAATCTCCC TGGATATGAC GATTCGAGCA AAAATTCAAA GTGCTCTCCG AGGAGATCCA
    GGTCATCCGA TTCTCATTCC ACAGTCAATT CCTGCGTTTA TTCAAGTTCC AAATCAAGGA
    GAATTGACCC TTCCAACATC AATCGGAAAT ACTATTATCA ATGACGGAAA TCCACGGATT
    AATACGACCG GGATGGAAAA ATCAGACGAA GCTCGGGCTT GGTTCGACTC GTTAACCTTG
    AATCTCGAAC AAGGAAAATC GCAAAACGAG AAAATGTGGA TCGCCACATC CGAACATCCA
    GGAGACCAAA ATGCGAAACT CTGGATAAAG ACTGCAAATA CGACGTATTC GGGAAGGCCG
    TACCTTCAAG TTGTCGGATT TATTGATAGA GCATTCCCAT CATTTTTGGC GAAAGTTTTC
    AAAGGAGGAG TCATTGCAGT GTATCTTTCA GTTATCCTCG TCGTTGGAAG AGGTCTGGTT
    CGTGGAATCT TCACCACATC TCCAAGCACT GTCATGTTCA CAGAATTACC GAATGCGGAT
    CATCTTCTCA AAATTTGTCT CGACATCTAT TTGGTTCGAG AAGCGAAGGA CTTCATGTTG
    GAGCAGGATC TCTTCGCCAA ATTAATATTC CTGTTCCGAT CACCAGCGAC ACTCATCGAA
    TGGACACGAA TGAGCAAAAA GAAGCAAGAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342180
    Clone ID Related Accession (Same CDS sequence) NM_001268460.2 , NM_001268460.3
    Accession Version NM_001268460.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Apr 20, 2015 this sequence version replaced NM_001268460.1. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACACCCGGAG ATAAGGAATC TGCTGCTTCG AAAGGAATGA TTGCAGTCAT GACATTTGTC
    ATTTTCCATT CGTATTCAAT CGCGCTGACT GCTATGATGA CATGGGCTCT CCTCTATCAT
    TCGATATTCG GTCTCATTCT CCTCATCCTC ACATGTATCT TGTGGATCTT CCGTGATACG
    AGAAAGTCAT CGTTTGCAAT GGCCCCTATT ATTTTGATGT ATATCGAGTT TCTATTGATT
    CTTCAATATT TCCTCAGTAT GGATATTCAT GCAGAAATTG GCGATCCAGC GTGGATGAAC
    TTTGTTGGAA TCGAATGGAC TACTCTTCCA GTACATGCTG TCATCATTCT CTGTGTCCAG
    ACTCTTCTCA CTCTCCCAGT GTTCTTGCTT CTACGTTTGG CTCGCAGGGA GAAGTTTTAC
    GAATCGCTGA GTGATTATGA ACGACAACGA CGAATCAACT CATATGGAAC ATTTGGAGCA
    TCAAAGACTG GAGCTGGGGG TGTTGCAGTG GCTAAGGATC CAAAAAGTCG GAAGTTCGCG
    GCATTTGTGG AATATCTGTC GAATAAAGTG TCTGTCTACT TCATATTTGT AGTATCAGTG
    GTACTTTTGG TGGTCTCTAC GTGTTTTGCT CCGAATTTCT ACAATATTCT ATTCTTCGCA
    CTATGGGCAT TGAACTTGAT TTATCTCAAG TTCTCATTCC GTCTGTATCG TGGATTGGCA
    TATGCATTCT GGCTAACTCT CACGTTCTAC ACTTCAATTG TTATTATCGC CCTCTACATT
    TATCAGTTCC CTGGAGTATC TCAATGGATT ATTCGAAATA CTAGTTTGTC GCAGGAGTGG
    CTGAATGCAA TTGGACTTGT CGATTTTAGA GCTATCGGTG AATCGGGAGC CCTATTTCTA
    CAATTACTCG CCCCAATTGC CTTGTTCGTC GTCACAATGC TCCAACTCAA GTTTTTCCAT
    GGACCATGGA GTCGAGCAAC GTCACCAAGA AGAGCAGAAA ATGATCCACC TACAAGTACG
    ACGGAGGCTG CGGCGGTAGC AAGCACATCT GGAACACAAG GAAGAGCACA TGCTGCAGGC
    GATACCCTTG TCAAGAAGCT GCACAAATTG GCAAATCAGA CAATCGAACT GTTATGGAGG
    TTTTTCGAAG TTCACATTTC GAAAATTGTC TTTGTCATCA TTGCGATTTT TATTGCCAAC
    AATATCAATG CTCTATACAT TCCACTTGTT ATTCTCCTCT CATTGGCTAT ATGTCTACCA
    TCAGCTGCAG ATGGGATATT CTCTTTATTT ATGTGTGCCT ACTTATTCCT CGTGGCACTG
    AGCAAGATGA TCTACCAACT GGATATTGTT CCCGAGTTAT CACAAATCGA CCGAGGAGTT
    GGAGCAGATA ACTGTTCACA TGGAAATATT TCAATGCCTG AATGGTTTGG ATTGAAGAAG
    GAAGTTGAAG GAACTGAACC AATTTATATG CTCTTTGGAG TCATTGTTTC GATTATTGCA
    TTGGCTTTTC AATCAATTGT TATATATCGA CAAAGACATT ATCGAGCTTC ACTGGGACTT
    CCTGAATCGA TGCGAGCTAA AGTGTTCCCG GACTTCCATC ATTCTCATTT TGATAGATCT
    CTCAAAAATG CCATCCAATT CTTGATAGAC TATGGTTTCT ATAAGTTTGG ACTTGAAATC
    ACGATGATCG CCATTGGAAT TGACATTTTC AATCGAATGG ATGCATTGGC AGCGATTCAA
    TGTTTCTGGC TTGTCCTGTT TGCGCTCAAT AAAAGAGTCT TTGTTCGAAG AATATGGGTA
    TTCTATGTGA TCTATATGGC GATTCTGTAT CCACTTCAGT TCTTTTCATA TGTTGGATTA
    CCACCTGACA GTTGTATTGA GTATCCATGG TCGTATTGGA TTCCATCGTA TAGTGATGAT
    GCGAGATTCA ATCTGTCGTA TCTTCTGAAC TTGTCAATAT ACGGTGTAAA CTGGCCATCA
    GCATATCTCA TTGGCGATTT CTTCGTACTC CTCTTGGCAT CATGCCAATT AGCAGTTTTC
    CGGCGAGAAG GAGAAGATAA TGATTCAATT TACAATGATG GAAACTTTGT GATTAAGCCA
    GAAAATCCAC AATATGACTT TATTGACACC AAAAAGAGTT ACGTTGATTA TTTCAAATCA
    TTCGTTTTCC ATTATGGACA CTGGATTACA TTGATGTCTA CACTAGCAGC AGGAATTGCT
    GGAACGTCAC TTTTTGCTTT GGGATACATT ATATTCACTC TGACAATGCT GTGGTCTGGT
    AACAATTTGT ATGTGATGAA CTCAACTCTA CGATCATTTG AACATACTTT GAAACGATGG
    AATGCACTTT TGGGATACAC ATTGTTCACG ATTACAATGA AAGTTTGCCT TCAAATATTT
    GGATGTGTAT TCCTGTCGTG GTTCGATCAA TCTGGTGGAT GGGGAAAGAC TCTGTGCATC
    GTTCGTCAAC TCTTCAGTAT CACATGTGTT AATAACGAAT GTCATGTGCT CAAAGAGCTG
    GAGGATTTCT CGAAGGCGTG TGCTGTTGAG ACGAAAGAAG GAAATATTGG ATTTGATGTG
    ATTGCGCTAT CCTTCCTCGT CTTCCAAATC CGAATTTTCC ATTCTTGGTA TTTCCAACAC
    TGCATGGTTG AATATCGAAG TGAGGTTATT CTGGCCAATA GAGGTGCCGT GCTGAAGAAT
    CAACTGATTG AAAAGGAAAT GAAGGAACAA AACGAGCAGC AAAAAGCCAA GTTCAATGAT
    ATCAGAAGAA GAACTGAAGC TATTCGTGAA AGATATCAAA AACAAATCGA ACGAGGAGCT
    GCTGAACGTG ACTTCGAGCC GGTTACTTAT GGGCACGCCA AACGAGCGGG AGACTACTAC
    ATGTTCAAAT ACGATCCTGA AAATGATGAT CTTGTGGAAC CCGTCGACAG TTTTGTACCA
    GAAGTTGATC CCAAGGCAAC TGCATATGAT CGACTTGATC CTGGACAGAT AATGTACGCT
    GCCACCGCTC ACGACCTCGA CCTCGCCAAA ACTGTACAGC AAGTCAAGAA AGGCGATACT
    ATCAAAGATC CTGATTCACG TGCTCTTATC GCTGTTTCTG AACCAGAAGC TCGTAAGCCA
    GGAGGCACTG AAGAAACGGA TGGTGATGAA GATGAGGATA ATAAGGATTC GAAAGTGGAA
    AGTACTGCTA AATTCATTCA AAAAATGATC GCTTCTGCCT TGGACTTGTG TAGTGTAACA
    CTAAACAAAC TGTGCAGAGA ACACCGATAT GTTGGATTTG TTTTGAGCAA GGAAAAACAA
    AAGTTGAAGT CAGGACACTC GGAATCTCTT TCAAATACAT CTAGAAAGCT GACGGATATT
    CGATCTGCAG TTGATCTACC AAGTCTTCAA CTTGTGCAAT CGGCGAATGA TGTTGAAAAG
    ATGGAGACAG CCGTCAGCGT GGATTGGCAA CAAAAATCAT CAGCAACGAG ACTATTGAAT
    GCAGTTGTGA ATTGTATTGG AGCACACACT GATATTCTCT GCTACTTCTT CGCAATTATG
    ACACAAGTGA TGACTGGTGG ACTTATTACT CTTCCACTTC CTCTGATGTC ACTATTCTGG
    GGAAATCTAT CGAATCCTCG TCCATCCAAG TTCTTCTGGG TCACAATGAT CACCTACACT
    GAGTGTGTCA TTGTTATCAA GTTTGTTTGC CAATTTGCAT TCATGCCTTA CAATTCTATC
    ACGTGGCGAA CAGAGCATCA AATGGACCCG ATGTCATTGG ATAAGTTATT CGGAGTATCA
    CAGAGAGATA GTTTTGCACT TTGGGACATT GTTTTGCTCT TCTCACTCTT CTTCCATCGG
    TATATGCTCA GAAAGTTAGG GTTATGGAAG GATGCCAATT TGACGGACAC GTTCACTTTG
    AAGGAAGAGC CTCGATCGGC GTCAGGATCG GACACAGGAT CACCCAAGAA AATTGCTCAA
    GAGCCAAAGG TTGTTGTAAC TCAGTCAGAT ACCCTTGAAG GTACAAGTGG AGGAGAGATA
    GTCATACCAT CTGATCCAAA TGCAGTTTCC AATATGGAAG AATTAGACTG CGAACCTCCG
    ATTCCAGAGA AGCAAAGTGG ACCAATTGGA AGATTCATTC ATCAATTATT CCATCCAAAA
    TTCCGTTACA TTCGTGATTT GTATCCAATA ATGTTTGGAA TCGATGTGAT ATGCTTCCTC
    ATCATGACAT TTGGATACTC GGCATTTGGT GAAGGAGGTT CTGGAAATGT TTTAGATGAT
    GTGAAGGCTT CTCGTATTCC GGTAACACTT GTTGTTATGC TCGTTGGAAT GACTCTGGCA
    ATTATCATTG ATCGAGCACT CTACCTCAGA AAATCAGTTG TCGGAAAGCT AATCTATCAA
    GTCTTAATGA TTGCTTTCCT TCACATTTGG GTATTCCTTG TTCTTCCAAA TATGACTCGT
    CGTTCTGCGA TATCGAATCA CGTTGCTCAA GCATTGTACG TCATCAAATC GTGCTATTTC
    CTTGTGTCTG CTTGGCAGAT CCGAAATGGT TATCCTGAAC TTTGTATTGG AAATCTATTA
    ACACATTCAT ACGGAATGAC CAATATGATT GCATTCAAAG TATTCATGAA TATTCCATTC
    CTCTTCGAGC TTCGTACAGC TATTGATTGG ACATGGACAG ATACTTCAAT GCCTCTTTTC
    GATTTCTTCA ATATGGAAAA TTTCTATGCT CATATATTCA ATATCAAGTG TGCCCGCCAG
    TTCGAAGCTG CCTATCCAGC TCCAAGAGGG ATTCCCAAAG GAAAGTTGGT CAAGTATATG
    ATGGGATTCC CAATTATCAT TGGAGTCGTT ATCTTCATCT TTTCTCCTCT ATTGCTCTGG
    TCGCTACTCA ATCAAATCGG AACTATCAGT ATGCCAGAAA AAGTTACGTT GAGGATTTCG
    ATTGAAGGAT ATCCACCACT TTACGAGATG GAGGCACAAG GAAGTAATCA CGATAACGCT
    GAACTTGGAA TGATAAAACC TGATCAATTG GCATCTTTGA ATCAAGCATT GACGGATAGT
    TACACGACAC GAGACACTAA TTCTATTTTA AGATCAAGAA TGTCTGTATC ATATCTGAAA
    GGATACACAT ATGAGGATAT TCTGATTGTG CGATTCCGTC CAGAATCTGA AATTTATTGG
    CCGATTTCAC AGGATTCGAG AAATGCGATG ATCGATAAAT TGAGCCGAAA CACGTCAGTC
    AATTTTGAAG TATCTCTGGA ATTCACTCGG CCATATGATC CAAATGAAAA CGCTGCTTTA
    AAACATTCGA AATCGTGGCT TGTTCCAATC TCCCTGGATA TGACGATTCG AGCAAAAATT
    CAAAGTGCTC TCCGAGGAGA TCCAGGTCAT CCGATTCTCA TTCCACAGTC AATTCCTGCG
    TTTATTCAAG TTCCAAATCA AGGAGAATTG ACCCTTCCAA CATCAATCGG AAATACTATT
    ATCAATGACG GAAATCCACG GATTAATACG ACCGGGATGG AAAAATCAGA CGAAGCTCGG
    GCTTGGTTCG ACTCGTTAAC CTTGAATCTC GAACAAGGAA AATCGCAAAA CGAGAAAATG
    TGGATCGCCA CATCCGAACA TCCAGGAGAC CAAAATGCGA AACTCTGGAT AAAGACTGCA
    AATACGACGT ATTCGGGAAG GCCGTACCTT CAAGTTGTCG GATTTATTGA TAGAGCATTC
    CCATCATTTT TGGCGAAAGT TTTCAAAGGA GGAGTCATTG CAGTGTATCT TTCAGTTATC
    CTCGTCGTTG GAAGAGGTCT GGTTCGTGGA ATCTTCACCA CATCTCCAAG CACTGTCATG
    TTCACAGAAT TACCGAATGC GGATCATCTT CTCAAAATTT GTCTCGACAT CTATTTGGTT
    CGAGAAGCGA AGGACTTCAT GTTGGAGCAG GATCTCTTCG CCAAATTAAT ATTCCTGTTC
    CGATCACCAG CGACACTCAT CGAATGGACA CGAATGAGCA AAAAGAAGCA AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255389.2
    CDS1..7317
    Translation

    Target ORF information:

    RefSeq Version NM_001268460.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268460.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACACCCGGAG ATAAGGAATC TGCTGCTTCG AAAGGAATGA TTGCAGTCAT GACATTTGTC
    ATTTTCCATT CGTATTCAAT CGCGCTGACT GCTATGATGA CATGGGCTCT CCTCTATCAT
    TCGATATTCG GTCTCATTCT CCTCATCCTC ACATGTATCT TGTGGATCTT CCGTGATACG
    AGAAAGTCAT CGTTTGCAAT GGCCCCTATT ATTTTGATGT ATATCGAGTT TCTATTGATT
    CTTCAATATT TCCTCAGTAT GGATATTCAT GCAGAAATTG GCGATCCAGC GTGGATGAAC
    TTTGTTGGAA TCGAATGGAC TACTCTTCCA GTACATGCTG TCATCATTCT CTGTGTCCAG
    ACTCTTCTCA CTCTCCCAGT GTTCTTGCTT CTACGTTTGG CTCGCAGGGA GAAGTTTTAC
    GAATCGCTGA GTGATTATGA ACGACAACGA CGAATCAACT CATATGGAAC ATTTGGAGCA
    TCAAAGACTG GAGCTGGGGG TGTTGCAGTG GCTAAGGATC CAAAAAGTCG GAAGTTCGCG
    GCATTTGTGG AATATCTGTC GAATAAAGTG TCTGTCTACT TCATATTTGT AGTATCAGTG
    GTACTTTTGG TGGTCTCTAC GTGTTTTGCT CCGAATTTCT ACAATATTCT ATTCTTCGCA
    CTATGGGCAT TGAACTTGAT TTATCTCAAG TTCTCATTCC GTCTGTATCG TGGATTGGCA
    TATGCATTCT GGCTAACTCT CACGTTCTAC ACTTCAATTG TTATTATCGC CCTCTACATT
    TATCAGTTCC CTGGAGTATC TCAATGGATT ATTCGAAATA CTAGTTTGTC GCAGGAGTGG
    CTGAATGCAA TTGGACTTGT CGATTTTAGA GCTATCGGTG AATCGGGAGC CCTATTTCTA
    CAATTACTCG CCCCAATTGC CTTGTTCGTC GTCACAATGC TCCAACTCAA GTTTTTCCAT
    GGACCATGGA GTCGAGCAAC GTCACCAAGA AGAGCAGAAA ATGATCCACC TACAAGTACG
    ACGGAGGCTG CGGCGGTAGC AAGCACATCT GGAACACAAG GAAGAGCACA TGCTGCAGGC
    GATACCCTTG TCAAGAAGCT GCACAAATTG GCAAATCAGA CAATCGAACT GTTATGGAGG
    TTTTTCGAAG TTCACATTTC GAAAATTGTC TTTGTCATCA TTGCGATTTT TATTGCCAAC
    AATATCAATG CTCTATACAT TCCACTTGTT ATTCTCCTCT CATTGGCTAT ATGTCTACCA
    TCAGCTGCAG ATGGGATATT CTCTTTATTT ATGTGTGCCT ACTTATTCCT CGTGGCACTG
    AGCAAGATGA TCTACCAACT GGATATTGTT CCCGAGTTAT CACAAATCGA CCGAGGAGTT
    GGAGCAGATA ACTGTTCACA TGGAAATATT TCAATGCCTG AATGGTTTGG ATTGAAGAAG
    GAAGTTGAAG GAACTGAACC AATTTATATG CTCTTTGGAG TCATTGTTTC GATTATTGCA
    TTGGCTTTTC AATCAATTGT TATATATCGA CAAAGACATT ATCGAGCTTC ACTGGGACTT
    CCTGAATCGA TGCGAGCTAA AGTGTTCCCG GACTTCCATC ATTCTCATTT TGATAGATCT
    CTCAAAAATG CCATCCAATT CTTGATAGAC TATGGTTTCT ATAAGTTTGG ACTTGAAATC
    ACGATGATCG CCATTGGAAT TGACATTTTC AATCGAATGG ATGCATTGGC AGCGATTCAA
    TGTTTCTGGC TTGTCCTGTT TGCGCTCAAT AAAAGAGTCT TTGTTCGAAG AATATGGGTA
    TTCTATGTGA TCTATATGGC GATTCTGTAT CCACTTCAGT TCTTTTCATA TGTTGGATTA
    CCACCTGACA GTTGTATTGA GTATCCATGG TCGTATTGGA TTCCATCGTA TAGTGATGAT
    GCGAGATTCA ATCTGTCGTA TCTTCTGAAC TTGTCAATAT ACGGTGTAAA CTGGCCATCA
    GCATATCTCA TTGGCGATTT CTTCGTACTC CTCTTGGCAT CATGCCAATT AGCAGTTTTC
    CGGCGAGAAG GAGAAGATAA TGATTCAATT TACAATGATG GAAACTTTGT GATTAAGCCA
    GAAAATCCAC AATATGACTT TATTGACACC AAAAAGAGTT ACGTTGATTA TTTCAAATCA
    TTCGTTTTCC ATTATGGACA CTGGATTACA TTGATGTCTA CACTAGCAGC AGGAATTGCT
    GGAACGTCAC TTTTTGCTTT GGGATACATT ATATTCACTC TGACAATGCT GTGGTCTGGT
    AACAATTTGT ATGTGATGAA CTCAACTCTA CGATCATTTG AACATACTTT GAAACGATGG
    AATGCACTTT TGGGATACAC ATTGTTCACG ATTACAATGA AAGTTTGCCT TCAAATATTT
    GGATGTGTAT TCCTGTCGTG GTTCGATCAA TCTGGTGGAT GGGGAAAGAC TCTGTGCATC
    GTTCGTCAAC TCTTCAGTAT CACATGTGTT AATAACGAAT GTCATGTGCT CAAAGAGCTG
    GAGGATTTCT CGAAGGCGTG TGCTGTTGAG ACGAAAGAAG GAAATATTGG ATTTGATGTG
    ATTGCGCTAT CCTTCCTCGT CTTCCAAATC CGAATTTTCC ATTCTTGGTA TTTCCAACAC
    TGCATGGTTG AATATCGAAG TGAGGTTATT CTGGCCAATA GAGGTGCCGT GCTGAAGAAT
    CAACTGATTG AAAAGGAAAT GAAGGAACAA AACGAGCAGC AAAAAGCCAA GTTCAATGAT
    ATCAGAAGAA GAACTGAAGC TATTCGTGAA AGATATCAAA AACAAATCGA ACGAGGAGCT
    GCTGAACGTG ACTTCGAGCC GGTTACTTAT GGGCACGCCA AACGAGCGGG AGACTACTAC
    ATGTTCAAAT ACGATCCTGA AAATGATGAT CTTGTGGAAC CCGTCGACAG TTTTGTACCA
    GAAGTTGATC CCAAGGCAAC TGCATATGAT CGACTTGATC CTGGACAGAT AATGTACGCT
    GCCACCGCTC ACGACCTCGA CCTCGCCAAA ACTGTACAGC AAGTCAAGAA AGGCGATACT
    ATCAAAGATC CTGATTCACG TGCTCTTATC GCTGTTTCTG AACCAGAAGC TCGTAAGCCA
    GGAGGCACTG AAGAAACGGA TGGTGATGAA GATGAGGATA ATAAGGATTC GAAAGTGGAA
    AGTACTGCTA AATTCATTCA AAAAATGATC GCTTCTGCCT TGGACTTGTG TAGTGTAACA
    CTAAACAAAC TGTGCAGAGA ACACCGATAT GTTGGATTTG TTTTGAGCAA GGAAAAACAA
    AAGTTGAAGT CAGGACACTC GGAATCTCTT TCAAATACAT CTAGAAAGCT GACGGATATT
    CGATCTGCAG TTGATCTACC AAGTCTTCAA CTTGTGCAAT CGGCGAATGA TGTTGAAAAG
    ATGGAGACAG CCGTCAGCGT GGATTGGCAA CAAAAATCAT CAGCAACGAG ACTATTGAAT
    GCAGTTGTGA ATTGTATTGG AGCACACACT GATATTCTCT GCTACTTCTT CGCAATTATG
    ACACAAGTGA TGACTGGTGG ACTTATTACT CTTCCACTTC CTCTGATGTC ACTATTCTGG
    GGAAATCTAT CGAATCCTCG TCCATCCAAG TTCTTCTGGG TCACAATGAT CACCTACACT
    GAGTGTGTCA TTGTTATCAA GTTTGTTTGC CAATTTGCAT TCATGCCTTA CAATTCTATC
    ACGTGGCGAA CAGAGCATCA AATGGACCCG ATGTCATTGG ATAAGTTATT CGGAGTATCA
    CAGAGAGATA GTTTTGCACT TTGGGACATT GTTTTGCTCT TCTCACTCTT CTTCCATCGG
    TATATGCTCA GAAAGTTAGG GTTATGGAAG GATGCCAATT TGACGGACAC GTTCACTTTG
    AAGGAAGAGC CTCGATCGGC GTCAGGATCG GACACAGGAT CACCCAAGAA AATTGCTCAA
    GAGCCAAAGG TTGTTGTAAC TCAGTCAGAT ACCCTTGAAG GTACAAGTGG AGGAGAGATA
    GTCATACCAT CTGATCCAAA TGCAGTTTCC AATATGGAAG AATTAGACTG CGAACCTCCG
    ATTCCAGAGA AGCAAAGTGG ACCAATTGGA AGATTCATTC ATCAATTATT CCATCCAAAA
    TTCCGTTACA TTCGTGATTT GTATCCAATA ATGTTTGGAA TCGATGTGAT ATGCTTCCTC
    ATCATGACAT TTGGATACTC GGCATTTGGT GAAGGAGGTT CTGGAAATGT TTTAGATGAT
    GTGAAGGCTT CTCGTATTCC GGTAACACTT GTTGTTATGC TCGTTGGAAT GACTCTGGCA
    ATTATCATTG ATCGAGCACT CTACCTCAGA AAATCAGTTG TCGGAAAGCT AATCTATCAA
    GTCTTAATGA TTGCTTTCCT TCACATTTGG GTATTCCTTG TTCTTCCAAA TATGACTCGT
    CGTTCTGCGA TATCGAATCA CGTTGCTCAA GCATTGTACG TCATCAAATC GTGCTATTTC
    CTTGTGTCTG CTTGGCAGAT CCGAAATGGT TATCCTGAAC TTTGTATTGG AAATCTATTA
    ACACATTCAT ACGGAATGAC CAATATGATT GCATTCAAAG TATTCATGAA TATTCCATTC
    CTCTTCGAGC TTCGTACAGC TATTGATTGG ACATGGACAG ATACTTCAAT GCCTCTTTTC
    GATTTCTTCA ATATGGAAAA TTTCTATGCT CATATATTCA ATATCAAGTG TGCCCGCCAG
    TTCGAAGCTG CCTATCCAGC TCCAAGAGGG ATTCCCAAAG GAAAGTTGGT CAAGTATATG
    ATGGGATTCC CAATTATCAT TGGAGTCGTT ATCTTCATCT TTTCTCCTCT ATTGCTCTGG
    TCGCTACTCA ATCAAATCGG AACTATCAGT ATGCCAGAAA AAGTTACGTT GAGGATTTCG
    ATTGAAGGAT ATCCACCACT TTACGAGATG GAGGCACAAG GAAGTAATCA CGATAACGCT
    GAACTTGGAA TGATAAAACC TGATCAATTG GCATCTTTGA ATCAAGCATT GACGGATAGT
    TACACGACAC GAGACACTAA TTCTATTTTA AGATCAAGAA TGTCTGTATC ATATCTGAAA
    GGATACACAT ATGAGGATAT TCTGATTGTG CGATTCCGTC CAGAATCTGA AATTTATTGG
    CCGATTTCAC AGGATTCGAG AAATGCGATG ATCGATAAAT TGAGCCGAAA CACGTCAGTC
    AATTTTGAAG TATCTCTGGA ATTCACTCGG CCATATGATC CAAATGAAAA CGCTGCTTTA
    AAACATTCGA AATCGTGGCT TGTTCCAATC TCCCTGGATA TGACGATTCG AGCAAAAATT
    CAAAGTGCTC TCCGAGGAGA TCCAGGTCAT CCGATTCTCA TTCCACAGTC AATTCCTGCG
    TTTATTCAAG TTCCAAATCA AGGAGAATTG ACCCTTCCAA CATCAATCGG AAATACTATT
    ATCAATGACG GAAATCCACG GATTAATACG ACCGGGATGG AAAAATCAGA CGAAGCTCGG
    GCTTGGTTCG ACTCGTTAAC CTTGAATCTC GAACAAGGAA AATCGCAAAA CGAGAAAATG
    TGGATCGCCA CATCCGAACA TCCAGGAGAC CAAAATGCGA AACTCTGGAT AAAGACTGCA
    AATACGACGT ATTCGGGAAG GCCGTACCTT CAAGTTGTCG GATTTATTGA TAGAGCATTC
    CCATCATTTT TGGCGAAAGT TTTCAAAGGA GGAGTCATTG CAGTGTATCT TTCAGTTATC
    CTCGTCGTTG GAAGAGGTCT GGTTCGTGGA ATCTTCACCA CATCTCCAAG CACTGTCATG
    TTCACAGAAT TACCGAATGC GGATCATCTT CTCAAAATTT GTCTCGACAT CTATTTGGTT
    CGAGAAGCGA AGGACTTCAT GTTGGAGCAG GATCTCTTCG CCAAATTAAT ATTCCTGTTC
    CGATCACCAG CGACACTCAT CGAATGGACA CGAATGAGCA AAAAGAAGCA AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342180
    Clone ID Related Accession (Same CDS sequence) NM_001268460.2 , NM_001268460.3
    Accession Version NM_001268460.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268460.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACACCCGGAG ATAAGGAATC TGCTGCTTCG AAAGGAATGA TTGCAGTCAT GACATTTGTC
    ATTTTCCATT CGTATTCAAT CGCGCTGACT GCTATGATGA CATGGGCTCT CCTCTATCAT
    TCGATATTCG GTCTCATTCT CCTCATCCTC ACATGTATCT TGTGGATCTT CCGTGATACG
    AGAAAGTCAT CGTTTGCAAT GGCCCCTATT ATTTTGATGT ATATCGAGTT TCTATTGATT
    CTTCAATATT TCCTCAGTAT GGATATTCAT GCAGAAATTG GCGATCCAGC GTGGATGAAC
    TTTGTTGGAA TCGAATGGAC TACTCTTCCA GTACATGCTG TCATCATTCT CTGTGTCCAG
    ACTCTTCTCA CTCTCCCAGT GTTCTTGCTT CTACGTTTGG CTCGCAGGGA GAAGTTTTAC
    GAATCGCTGA GTGATTATGA ACGACAACGA CGAATCAACT CATATGGAAC ATTTGGAGCA
    TCAAAGACTG GAGCTGGGGG TGTTGCAGTG GCTAAGGATC CAAAAAGTCG GAAGTTCGCG
    GCATTTGTGG AATATCTGTC GAATAAAGTG TCTGTCTACT TCATATTTGT AGTATCAGTG
    GTACTTTTGG TGGTCTCTAC GTGTTTTGCT CCGAATTTCT ACAATATTCT ATTCTTCGCA
    CTATGGGCAT TGAACTTGAT TTATCTCAAG TTCTCATTCC GTCTGTATCG TGGATTGGCA
    TATGCATTCT GGCTAACTCT CACGTTCTAC ACTTCAATTG TTATTATCGC CCTCTACATT
    TATCAGTTCC CTGGAGTATC TCAATGGATT ATTCGAAATA CTAGTTTGTC GCAGGAGTGG
    CTGAATGCAA TTGGACTTGT CGATTTTAGA GCTATCGGTG AATCGGGAGC CCTATTTCTA
    CAATTACTCG CCCCAATTGC CTTGTTCGTC GTCACAATGC TCCAACTCAA GTTTTTCCAT
    GGACCATGGA GTCGAGCAAC GTCACCAAGA AGAGCAGAAA ATGATCCACC TACAAGTACG
    ACGGAGGCTG CGGCGGTAGC AAGCACATCT GGAACACAAG GAAGAGCACA TGCTGCAGGC
    GATACCCTTG TCAAGAAGCT GCACAAATTG GCAAATCAGA CAATCGAACT GTTATGGAGG
    TTTTTCGAAG TTCACATTTC GAAAATTGTC TTTGTCATCA TTGCGATTTT TATTGCCAAC
    AATATCAATG CTCTATACAT TCCACTTGTT ATTCTCCTCT CATTGGCTAT ATGTCTACCA
    TCAGCTGCAG ATGGGATATT CTCTTTATTT ATGTGTGCCT ACTTATTCCT CGTGGCACTG
    AGCAAGATGA TCTACCAACT GGATATTGTT CCCGAGTTAT CACAAATCGA CCGAGGAGTT
    GGAGCAGATA ACTGTTCACA TGGAAATATT TCAATGCCTG AATGGTTTGG ATTGAAGAAG
    GAAGTTGAAG GAACTGAACC AATTTATATG CTCTTTGGAG TCATTGTTTC GATTATTGCA
    TTGGCTTTTC AATCAATTGT TATATATCGA CAAAGACATT ATCGAGCTTC ACTGGGACTT
    CCTGAATCGA TGCGAGCTAA AGTGTTCCCG GACTTCCATC ATTCTCATTT TGATAGATCT
    CTCAAAAATG CCATCCAATT CTTGATAGAC TATGGTTTCT ATAAGTTTGG ACTTGAAATC
    ACGATGATCG CCATTGGAAT TGACATTTTC AATCGAATGG ATGCATTGGC AGCGATTCAA
    TGTTTCTGGC TTGTCCTGTT TGCGCTCAAT AAAAGAGTCT TTGTTCGAAG AATATGGGTA
    TTCTATGTGA TCTATATGGC GATTCTGTAT CCACTTCAGT TCTTTTCATA TGTTGGATTA
    CCACCTGACA GTTGTATTGA GTATCCATGG TCGTATTGGA TTCCATCGTA TAGTGATGAT
    GCGAGATTCA ATCTGTCGTA TCTTCTGAAC TTGTCAATAT ACGGTGTAAA CTGGCCATCA
    GCATATCTCA TTGGCGATTT CTTCGTACTC CTCTTGGCAT CATGCCAATT AGCAGTTTTC
    CGGCGAGAAG GAGAAGATAA TGATTCAATT TACAATGATG GAAACTTTGT GATTAAGCCA
    GAAAATCCAC AATATGACTT TATTGACACC AAAAAGAGTT ACGTTGATTA TTTCAAATCA
    TTCGTTTTCC ATTATGGACA CTGGATTACA TTGATGTCTA CACTAGCAGC AGGAATTGCT
    GGAACGTCAC TTTTTGCTTT GGGATACATT ATATTCACTC TGACAATGCT GTGGTCTGGT
    AACAATTTGT ATGTGATGAA CTCAACTCTA CGATCATTTG AACATACTTT GAAACGATGG
    AATGCACTTT TGGGATACAC ATTGTTCACG ATTACAATGA AAGTTTGCCT TCAAATATTT
    GGATGTGTAT TCCTGTCGTG GTTCGATCAA TCTGGTGGAT GGGGAAAGAC TCTGTGCATC
    GTTCGTCAAC TCTTCAGTAT CACATGTGTT AATAACGAAT GTCATGTGCT CAAAGAGCTG
    GAGGATTTCT CGAAGGCGTG TGCTGTTGAG ACGAAAGAAG GAAATATTGG ATTTGATGTG
    ATTGCGCTAT CCTTCCTCGT CTTCCAAATC CGAATTTTCC ATTCTTGGTA TTTCCAACAC
    TGCATGGTTG AATATCGAAG TGAGGTTATT CTGGCCAATA GAGGTGCCGT GCTGAAGAAT
    CAACTGATTG AAAAGGAAAT GAAGGAACAA AACGAGCAGC AAAAAGCCAA GTTCAATGAT
    ATCAGAAGAA GAACTGAAGC TATTCGTGAA AGATATCAAA AACAAATCGA ACGAGGAGCT
    GCTGAACGTG ACTTCGAGCC GGTTACTTAT GGGCACGCCA AACGAGCGGG AGACTACTAC
    ATGTTCAAAT ACGATCCTGA AAATGATGAT CTTGTGGAAC CCGTCGACAG TTTTGTACCA
    GAAGTTGATC CCAAGGCAAC TGCATATGAT CGACTTGATC CTGGACAGAT AATGTACGCT
    GCCACCGCTC ACGACCTCGA CCTCGCCAAA ACTGTACAGC AAGTCAAGAA AGGCGATACT
    ATCAAAGATC CTGATTCACG TGCTCTTATC GCTGTTTCTG AACCAGAAGC TCGTAAGCCA
    GGAGGCACTG AAGAAACGGA TGGTGATGAA GATGAGGATA ATAAGGATTC GAAAGTGGAA
    AGTACTGCTA AATTCATTCA AAAAATGATC GCTTCTGCCT TGGACTTGTG TAGTGTAACA
    CTAAACAAAC TGTGCAGAGA ACACCGATAT GTTGGATTTG TTTTGAGCAA GGAAAAACAA
    AAGTTGAAGT CAGGACACTC GGAATCTCTT TCAAATACAT CTAGAAAGCT GACGGATATT
    CGATCTGCAG TTGATCTACC AAGTCTTCAA CTTGTGCAAT CGGCGAATGA TGTTGAAAAG
    ATGGAGACAG CCGTCAGCGT GGATTGGCAA CAAAAATCAT CAGCAACGAG ACTATTGAAT
    GCAGTTGTGA ATTGTATTGG AGCACACACT GATATTCTCT GCTACTTCTT CGCAATTATG
    ACACAAGTGA TGACTGGTGG ACTTATTACT CTTCCACTTC CTCTGATGTC ACTATTCTGG
    GGAAATCTAT CGAATCCTCG TCCATCCAAG TTCTTCTGGG TCACAATGAT CACCTACACT
    GAGTGTGTCA TTGTTATCAA GTTTGTTTGC CAATTTGCAT TCATGCCTTA CAATTCTATC
    ACGTGGCGAA CAGAGCATCA AATGGACCCG ATGTCATTGG ATAAGTTATT CGGAGTATCA
    CAGAGAGATA GTTTTGCACT TTGGGACATT GTTTTGCTCT TCTCACTCTT CTTCCATCGG
    TATATGCTCA GAAAGTTAGG GTTATGGAAG GATGCCAATT TGACGGACAC GTTCACTTTG
    AAGGAAGAGC CTCGATCGGC GTCAGGATCG GACACAGGAT CACCCAAGAA AATTGCTCAA
    GAGCCAAAGG TTGTTGTAAC TCAGTCAGAT ACCCTTGAAG GTACAAGTGG AGGAGAGATA
    GTCATACCAT CTGATCCAAA TGCAGTTTCC AATATGGAAG AATTAGACTG CGAACCTCCG
    ATTCCAGAGA AGCAAAGTGG ACCAATTGGA AGATTCATTC ATCAATTATT CCATCCAAAA
    TTCCGTTACA TTCGTGATTT GTATCCAATA ATGTTTGGAA TCGATGTGAT ATGCTTCCTC
    ATCATGACAT TTGGATACTC GGCATTTGGT GAAGGAGGTT CTGGAAATGT TTTAGATGAT
    GTGAAGGCTT CTCGTATTCC GGTAACACTT GTTGTTATGC TCGTTGGAAT GACTCTGGCA
    ATTATCATTG ATCGAGCACT CTACCTCAGA AAATCAGTTG TCGGAAAGCT AATCTATCAA
    GTCTTAATGA TTGCTTTCCT TCACATTTGG GTATTCCTTG TTCTTCCAAA TATGACTCGT
    CGTTCTGCGA TATCGAATCA CGTTGCTCAA GCATTGTACG TCATCAAATC GTGCTATTTC
    CTTGTGTCTG CTTGGCAGAT CCGAAATGGT TATCCTGAAC TTTGTATTGG AAATCTATTA
    ACACATTCAT ACGGAATGAC CAATATGATT GCATTCAAAG TATTCATGAA TATTCCATTC
    CTCTTCGAGC TTCGTACAGC TATTGATTGG ACATGGACAG ATACTTCAAT GCCTCTTTTC
    GATTTCTTCA ATATGGAAAA TTTCTATGCT CATATATTCA ATATCAAGTG TGCCCGCCAG
    TTCGAAGCTG CCTATCCAGC TCCAAGAGGG ATTCCCAAAG GAAAGTTGGT CAAGTATATG
    ATGGGATTCC CAATTATCAT TGGAGTCGTT ATCTTCATCT TTTCTCCTCT ATTGCTCTGG
    TCGCTACTCA ATCAAATCGG AACTATCAGT ATGCCAGAAA AAGTTACGTT GAGGATTTCG
    ATTGAAGGAT ATCCACCACT TTACGAGATG GAGGCACAAG GAAGTAATCA CGATAACGCT
    GAACTTGGAA TGATAAAACC TGATCAATTG GCATCTTTGA ATCAAGCATT GACGGATAGT
    TACACGACAC GAGACACTAA TTCTATTTTA AGATCAAGAA TGTCTGTATC ATATCTGAAA
    GGATACACAT ATGAGGATAT TCTGATTGTG CGATTCCGTC CAGAATCTGA AATTTATTGG
    CCGATTTCAC AGGATTCGAG AAATGCGATG ATCGATAAAT TGAGCCGAAA CACGTCAGTC
    AATTTTGAAG TATCTCTGGA ATTCACTCGG CCATATGATC CAAATGAAAA CGCTGCTTTA
    AAACATTCGA AATCGTGGCT TGTTCCAATC TCCCTGGATA TGACGATTCG AGCAAAAATT
    CAAAGTGCTC TCCGAGGAGA TCCAGGTCAT CCGATTCTCA TTCCACAGTC AATTCCTGCG
    TTTATTCAAG TTCCAAATCA AGGAGAATTG ACCCTTCCAA CATCAATCGG AAATACTATT
    ATCAATGACG GAAATCCACG GATTAATACG ACCGGGATGG AAAAATCAGA CGAAGCTCGG
    GCTTGGTTCG ACTCGTTAAC CTTGAATCTC GAACAAGGAA AATCGCAAAA CGAGAAAATG
    TGGATCGCCA CATCCGAACA TCCAGGAGAC CAAAATGCGA AACTCTGGAT AAAGACTGCA
    AATACGACGT ATTCGGGAAG GCCGTACCTT CAAGTTGTCG GATTTATTGA TAGAGCATTC
    CCATCATTTT TGGCGAAAGT TTTCAAAGGA GGAGTCATTG CAGTGTATCT TTCAGTTATC
    CTCGTCGTTG GAAGAGGTCT GGTTCGTGGA ATCTTCACCA CATCTCCAAG CACTGTCATG
    TTCACAGAAT TACCGAATGC GGATCATCTT CTCAAAATTT GTCTCGACAT CTATTTGGTT
    CGAGAAGCGA AGGACTTCAT GTTGGAGCAG GATCTCTTCG CCAAATTAAT ATTCCTGTTC
    CGATCACCAG CGACACTCAT CGAATGGACA CGAATGAGCA AAAAGAAGCA AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255389.2
    CDS57..7373
    Translation

    Target ORF information:

    RefSeq Version NM_001268460.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268460.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACACCCGGAG ATAAGGAATC TGCTGCTTCG AAAGGAATGA TTGCAGTCAT GACATTTGTC
    ATTTTCCATT CGTATTCAAT CGCGCTGACT GCTATGATGA CATGGGCTCT CCTCTATCAT
    TCGATATTCG GTCTCATTCT CCTCATCCTC ACATGTATCT TGTGGATCTT CCGTGATACG
    AGAAAGTCAT CGTTTGCAAT GGCCCCTATT ATTTTGATGT ATATCGAGTT TCTATTGATT
    CTTCAATATT TCCTCAGTAT GGATATTCAT GCAGAAATTG GCGATCCAGC GTGGATGAAC
    TTTGTTGGAA TCGAATGGAC TACTCTTCCA GTACATGCTG TCATCATTCT CTGTGTCCAG
    ACTCTTCTCA CTCTCCCAGT GTTCTTGCTT CTACGTTTGG CTCGCAGGGA GAAGTTTTAC
    GAATCGCTGA GTGATTATGA ACGACAACGA CGAATCAACT CATATGGAAC ATTTGGAGCA
    TCAAAGACTG GAGCTGGGGG TGTTGCAGTG GCTAAGGATC CAAAAAGTCG GAAGTTCGCG
    GCATTTGTGG AATATCTGTC GAATAAAGTG TCTGTCTACT TCATATTTGT AGTATCAGTG
    GTACTTTTGG TGGTCTCTAC GTGTTTTGCT CCGAATTTCT ACAATATTCT ATTCTTCGCA
    CTATGGGCAT TGAACTTGAT TTATCTCAAG TTCTCATTCC GTCTGTATCG TGGATTGGCA
    TATGCATTCT GGCTAACTCT CACGTTCTAC ACTTCAATTG TTATTATCGC CCTCTACATT
    TATCAGTTCC CTGGAGTATC TCAATGGATT ATTCGAAATA CTAGTTTGTC GCAGGAGTGG
    CTGAATGCAA TTGGACTTGT CGATTTTAGA GCTATCGGTG AATCGGGAGC CCTATTTCTA
    CAATTACTCG CCCCAATTGC CTTGTTCGTC GTCACAATGC TCCAACTCAA GTTTTTCCAT
    GGACCATGGA GTCGAGCAAC GTCACCAAGA AGAGCAGAAA ATGATCCACC TACAAGTACG
    ACGGAGGCTG CGGCGGTAGC AAGCACATCT GGAACACAAG GAAGAGCACA TGCTGCAGGC
    GATACCCTTG TCAAGAAGCT GCACAAATTG GCAAATCAGA CAATCGAACT GTTATGGAGG
    TTTTTCGAAG TTCACATTTC GAAAATTGTC TTTGTCATCA TTGCGATTTT TATTGCCAAC
    AATATCAATG CTCTATACAT TCCACTTGTT ATTCTCCTCT CATTGGCTAT ATGTCTACCA
    TCAGCTGCAG ATGGGATATT CTCTTTATTT ATGTGTGCCT ACTTATTCCT CGTGGCACTG
    AGCAAGATGA TCTACCAACT GGATATTGTT CCCGAGTTAT CACAAATCGA CCGAGGAGTT
    GGAGCAGATA ACTGTTCACA TGGAAATATT TCAATGCCTG AATGGTTTGG ATTGAAGAAG
    GAAGTTGAAG GAACTGAACC AATTTATATG CTCTTTGGAG TCATTGTTTC GATTATTGCA
    TTGGCTTTTC AATCAATTGT TATATATCGA CAAAGACATT ATCGAGCTTC ACTGGGACTT
    CCTGAATCGA TGCGAGCTAA AGTGTTCCCG GACTTCCATC ATTCTCATTT TGATAGATCT
    CTCAAAAATG CCATCCAATT CTTGATAGAC TATGGTTTCT ATAAGTTTGG ACTTGAAATC
    ACGATGATCG CCATTGGAAT TGACATTTTC AATCGAATGG ATGCATTGGC AGCGATTCAA
    TGTTTCTGGC TTGTCCTGTT TGCGCTCAAT AAAAGAGTCT TTGTTCGAAG AATATGGGTA
    TTCTATGTGA TCTATATGGC GATTCTGTAT CCACTTCAGT TCTTTTCATA TGTTGGATTA
    CCACCTGACA GTTGTATTGA GTATCCATGG TCGTATTGGA TTCCATCGTA TAGTGATGAT
    GCGAGATTCA ATCTGTCGTA TCTTCTGAAC TTGTCAATAT ACGGTGTAAA CTGGCCATCA
    GCATATCTCA TTGGCGATTT CTTCGTACTC CTCTTGGCAT CATGCCAATT AGCAGTTTTC
    CGGCGAGAAG GAGAAGATAA TGATTCAATT TACAATGATG GAAACTTTGT GATTAAGCCA
    GAAAATCCAC AATATGACTT TATTGACACC AAAAAGAGTT ACGTTGATTA TTTCAAATCA
    TTCGTTTTCC ATTATGGACA CTGGATTACA TTGATGTCTA CACTAGCAGC AGGAATTGCT
    GGAACGTCAC TTTTTGCTTT GGGATACATT ATATTCACTC TGACAATGCT GTGGTCTGGT
    AACAATTTGT ATGTGATGAA CTCAACTCTA CGATCATTTG AACATACTTT GAAACGATGG
    AATGCACTTT TGGGATACAC ATTGTTCACG ATTACAATGA AAGTTTGCCT TCAAATATTT
    GGATGTGTAT TCCTGTCGTG GTTCGATCAA TCTGGTGGAT GGGGAAAGAC TCTGTGCATC
    GTTCGTCAAC TCTTCAGTAT CACATGTGTT AATAACGAAT GTCATGTGCT CAAAGAGCTG
    GAGGATTTCT CGAAGGCGTG TGCTGTTGAG ACGAAAGAAG GAAATATTGG ATTTGATGTG
    ATTGCGCTAT CCTTCCTCGT CTTCCAAATC CGAATTTTCC ATTCTTGGTA TTTCCAACAC
    TGCATGGTTG AATATCGAAG TGAGGTTATT CTGGCCAATA GAGGTGCCGT GCTGAAGAAT
    CAACTGATTG AAAAGGAAAT GAAGGAACAA AACGAGCAGC AAAAAGCCAA GTTCAATGAT
    ATCAGAAGAA GAACTGAAGC TATTCGTGAA AGATATCAAA AACAAATCGA ACGAGGAGCT
    GCTGAACGTG ACTTCGAGCC GGTTACTTAT GGGCACGCCA AACGAGCGGG AGACTACTAC
    ATGTTCAAAT ACGATCCTGA AAATGATGAT CTTGTGGAAC CCGTCGACAG TTTTGTACCA
    GAAGTTGATC CCAAGGCAAC TGCATATGAT CGACTTGATC CTGGACAGAT AATGTACGCT
    GCCACCGCTC ACGACCTCGA CCTCGCCAAA ACTGTACAGC AAGTCAAGAA AGGCGATACT
    ATCAAAGATC CTGATTCACG TGCTCTTATC GCTGTTTCTG AACCAGAAGC TCGTAAGCCA
    GGAGGCACTG AAGAAACGGA TGGTGATGAA GATGAGGATA ATAAGGATTC GAAAGTGGAA
    AGTACTGCTA AATTCATTCA AAAAATGATC GCTTCTGCCT TGGACTTGTG TAGTGTAACA
    CTAAACAAAC TGTGCAGAGA ACACCGATAT GTTGGATTTG TTTTGAGCAA GGAAAAACAA
    AAGTTGAAGT CAGGACACTC GGAATCTCTT TCAAATACAT CTAGAAAGCT GACGGATATT
    CGATCTGCAG TTGATCTACC AAGTCTTCAA CTTGTGCAAT CGGCGAATGA TGTTGAAAAG
    ATGGAGACAG CCGTCAGCGT GGATTGGCAA CAAAAATCAT CAGCAACGAG ACTATTGAAT
    GCAGTTGTGA ATTGTATTGG AGCACACACT GATATTCTCT GCTACTTCTT CGCAATTATG
    ACACAAGTGA TGACTGGTGG ACTTATTACT CTTCCACTTC CTCTGATGTC ACTATTCTGG
    GGAAATCTAT CGAATCCTCG TCCATCCAAG TTCTTCTGGG TCACAATGAT CACCTACACT
    GAGTGTGTCA TTGTTATCAA GTTTGTTTGC CAATTTGCAT TCATGCCTTA CAATTCTATC
    ACGTGGCGAA CAGAGCATCA AATGGACCCG ATGTCATTGG ATAAGTTATT CGGAGTATCA
    CAGAGAGATA GTTTTGCACT TTGGGACATT GTTTTGCTCT TCTCACTCTT CTTCCATCGG
    TATATGCTCA GAAAGTTAGG GTTATGGAAG GATGCCAATT TGACGGACAC GTTCACTTTG
    AAGGAAGAGC CTCGATCGGC GTCAGGATCG GACACAGGAT CACCCAAGAA AATTGCTCAA
    GAGCCAAAGG TTGTTGTAAC TCAGTCAGAT ACCCTTGAAG GTACAAGTGG AGGAGAGATA
    GTCATACCAT CTGATCCAAA TGCAGTTTCC AATATGGAAG AATTAGACTG CGAACCTCCG
    ATTCCAGAGA AGCAAAGTGG ACCAATTGGA AGATTCATTC ATCAATTATT CCATCCAAAA
    TTCCGTTACA TTCGTGATTT GTATCCAATA ATGTTTGGAA TCGATGTGAT ATGCTTCCTC
    ATCATGACAT TTGGATACTC GGCATTTGGT GAAGGAGGTT CTGGAAATGT TTTAGATGAT
    GTGAAGGCTT CTCGTATTCC GGTAACACTT GTTGTTATGC TCGTTGGAAT GACTCTGGCA
    ATTATCATTG ATCGAGCACT CTACCTCAGA AAATCAGTTG TCGGAAAGCT AATCTATCAA
    GTCTTAATGA TTGCTTTCCT TCACATTTGG GTATTCCTTG TTCTTCCAAA TATGACTCGT
    CGTTCTGCGA TATCGAATCA CGTTGCTCAA GCATTGTACG TCATCAAATC GTGCTATTTC
    CTTGTGTCTG CTTGGCAGAT CCGAAATGGT TATCCTGAAC TTTGTATTGG AAATCTATTA
    ACACATTCAT ACGGAATGAC CAATATGATT GCATTCAAAG TATTCATGAA TATTCCATTC
    CTCTTCGAGC TTCGTACAGC TATTGATTGG ACATGGACAG ATACTTCAAT GCCTCTTTTC
    GATTTCTTCA ATATGGAAAA TTTCTATGCT CATATATTCA ATATCAAGTG TGCCCGCCAG
    TTCGAAGCTG CCTATCCAGC TCCAAGAGGG ATTCCCAAAG GAAAGTTGGT CAAGTATATG
    ATGGGATTCC CAATTATCAT TGGAGTCGTT ATCTTCATCT TTTCTCCTCT ATTGCTCTGG
    TCGCTACTCA ATCAAATCGG AACTATCAGT ATGCCAGAAA AAGTTACGTT GAGGATTTCG
    ATTGAAGGAT ATCCACCACT TTACGAGATG GAGGCACAAG GAAGTAATCA CGATAACGCT
    GAACTTGGAA TGATAAAACC TGATCAATTG GCATCTTTGA ATCAAGCATT GACGGATAGT
    TACACGACAC GAGACACTAA TTCTATTTTA AGATCAAGAA TGTCTGTATC ATATCTGAAA
    GGATACACAT ATGAGGATAT TCTGATTGTG CGATTCCGTC CAGAATCTGA AATTTATTGG
    CCGATTTCAC AGGATTCGAG AAATGCGATG ATCGATAAAT TGAGCCGAAA CACGTCAGTC
    AATTTTGAAG TATCTCTGGA ATTCACTCGG CCATATGATC CAAATGAAAA CGCTGCTTTA
    AAACATTCGA AATCGTGGCT TGTTCCAATC TCCCTGGATA TGACGATTCG AGCAAAAATT
    CAAAGTGCTC TCCGAGGAGA TCCAGGTCAT CCGATTCTCA TTCCACAGTC AATTCCTGCG
    TTTATTCAAG TTCCAAATCA AGGAGAATTG ACCCTTCCAA CATCAATCGG AAATACTATT
    ATCAATGACG GAAATCCACG GATTAATACG ACCGGGATGG AAAAATCAGA CGAAGCTCGG
    GCTTGGTTCG ACTCGTTAAC CTTGAATCTC GAACAAGGAA AATCGCAAAA CGAGAAAATG
    TGGATCGCCA CATCCGAACA TCCAGGAGAC CAAAATGCGA AACTCTGGAT AAAGACTGCA
    AATACGACGT ATTCGGGAAG GCCGTACCTT CAAGTTGTCG GATTTATTGA TAGAGCATTC
    CCATCATTTT TGGCGAAAGT TTTCAAAGGA GGAGTCATTG CAGTGTATCT TTCAGTTATC
    CTCGTCGTTG GAAGAGGTCT GGTTCGTGGA ATCTTCACCA CATCTCCAAG CACTGTCATG
    TTCACAGAAT TACCGAATGC GGATCATCTT CTCAAAATTT GTCTCGACAT CTATTTGGTT
    CGAGAAGCGA AGGACTTCAT GTTGGAGCAG GATCTCTTCG CCAAATTAAT ATTCCTGTTC
    CGATCACCAG CGACACTCAT CGAATGGACA CGAATGAGCA AAAAGAAGCA AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342181
    Clone ID Related Accession (Same CDS sequence) NM_001268461.2 , NM_001268461.3
    Accession Version NM_001268461.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Apr 20, 2015 this sequence version replaced NM_001268461.1. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGGATCCAAA AAGTCGGAAG
    TTCGCGGCAT TTGTGGAATA TCTGTCGAAT AAAGTGTCTG TCTACTTCAT ATTTGTAGTA
    TCAGTGGTAC TTTTGGTGGT CTCTACGTGT TTTGCTCCGA ATTTCTACAA TATTCTATTC
    TTCGCACTAT GGGCATTGAA CTTGATTTAT CTCAAGTTCT CATTCCGTCT GTATCGTGGA
    TTGGCATATG CATTCTGGCT AACTCTCACG TTCTACACTT CAATTGTTAT TATCGCCCTC
    TACATTTATC AGTTCCCTGG AGTATCTCAA TGGATTATTC GAAATACTAG TTTGTCGCAG
    GAGTGGCTGA ATGCAATTGG ACTTGTCGAT TTTAGAGCTA TCGGTGAATC GGGAGCCCTA
    TTTCTACAAT TACTCGCCCC AATTGCCTTG TTCGTCGTCA CAATGCTCCA ACTCAAGTTT
    TTCCATGGAC CATGGAGTCG AGCAACGTCA CCAAGAAGAG CAGAAAATGA TCCACCTACA
    AGTACGACGG AGGCTGCGGC GGTAGCAAGC ACATCTGGAA CACAAGGAAG AGCACATGCT
    GCAGGCGATA CCCTTGTCAA GAAGCTGCAC AAATTGGCAA ATCAGACAAT CGAACTGTTA
    TGGAGGTTTT TCGAAGTTCA CATTTCGAAA ATTGTCTTTG TCATCATTGC GATTTTTATT
    GCCAACAATA TCAATGCTCT ATACATTCCA CTTGTTATTC TCCTCTCATT GGCTATATGT
    CTACCATCAG CTGCAGATGG GATATTCTCT TTATTTATGT GTGCCTACTT ATTCCTCGTG
    GCACTGAGCA AGATGATCTA CCAACTGGAT ATTGTTCCCG AGTTATCACA AATCGACCGA
    GGAGTTGGAG CAGATAACTG TTCACATGGA AATATTTCAA TGCCTGAATG GTTTGGATTG
    AAGAAGGAAG TTGAAGGAAC TGAACCAATT TATATGCTCT TTGGAGTCAT TGTTTCGATT
    ATTGCATTGG CTTTTCAATC AATTGTTATA TATCGACAAA GACATTATCG AGCTTCACTG
    GGACTTCCTG AATCGATGCG AGCTAAAGTG TTCCCGGACT TCCATCATTC TCATTTTGAT
    AGATCTCTCA AAAATGCCAT CCAATTCTTG ATAGACTATG GTTTCTATAA GTTTGGACTT
    GAAATCACGA TGATCGCCAT TGGAATTGAC ATTTTCAATC GAATGGATGC ATTGGCAGCG
    ATTCAATGTT TCTGGCTTGT CCTGTTTGCG CTCAATAAAA GAGTCTTTGT TCGAAGAATA
    TGGGTATTCT ATGTGATCTA TATGGCGATT CTGTATCCAC TTCAGTTCTT TTCATATGTT
    GGATTACCAC CTGACAGTTG TATTGAGTAT CCATGGTCGT ATTGGATTCC ATCGTATAGT
    GATGATGCGA GATTCAATCT GTCGTATCTT CTGAACTTGT CAATATACGG TGTAAACTGG
    CCATCAGCAT ATCTCATTGG CGATTTCTTC GTACTCCTCT TGGCATCATG CCAATTAGCA
    GTTTTCCGGC GAGAAGGAGA AGATAATGAT TCAATTTACA ATGATGGAAA CTTTGTGATT
    AAGCCAGAAA ATCCACAATA TGACTTTATT GACACCAAAA AGAGTTACGT TGATTATTTC
    AAATCATTCG TTTTCCATTA TGGACACTGG ATTACATTGA TGTCTACACT AGCAGCAGGA
    ATTGCTGGAA CGTCACTTTT TGCTTTGGGA TACATTATAT TCACTCTGAC AATGCTGTGG
    TCTGGTAACA ATTTGTATGT GATGAACTCA ACTCTACGAT CATTTGAACA TACTTTGAAA
    CGATGGAATG CACTTTTGGG ATACACATTG TTCACGATTA CAATGAAAGT TTGCCTTCAA
    ATATTTGGAT GTGTATTCCT GTCGTGGTTC GATCAATCTG GTGGATGGGG AAAGACTCTG
    TGCATCGTTC GTCAACTCTT CAGTATCACA TGTGTTAATA ACGAATGTCA TGTGCTCAAA
    GAGCTGGAGG ATTTCTCGAA GGCGTGTGCT GTTGAGACGA AAGAAGGAAA TATTGGATTT
    GATGTGATTG CGCTATCCTT CCTCGTCTTC CAAATCCGAA TTTTCCATTC TTGGTATTTC
    CAACACTGCA TGGTTGAATA TCGAAGTGAG GTTATTCTGG CCAATAGAGG TGCCGTGCTG
    AAGAATCAAC TGATTGAAAA GGAAATGAAG GAACAAAACG AGCAGCAAAA AGCCAAGTTC
    AATGATATCA GAAGAAGAAC TGAAGCTATT CGTGAAAGAT ATCAAAAACA AATCGAACGA
    GGAGCTGCTG AACGTGACTT CGAGCCGGTT ACTTATGGGC ACGCCAAACG AGCGGGAGAC
    TACTACATGT TCAAATACGA TCCTGAAAAT GATGATCTTG TGGAACCCGT CGACAGTTTT
    GTACCAGAAG TTGATCCCAA GGCAACTGCA TATGATCGAC TTGATCCTGG ACAGATAATG
    TACGCTGCCA CCGCTCACGA CCTCGACCTC GCCAAAACTG TACAGCAAGT CAAGAAAGGC
    GATACTATCA AAGATCCTGA TTCACGTGCT CTTATCGCTG TTTCTGAACC AGAAGCTCGT
    AAGCCAGGAG GCACTGAAGA AACGGATGGT GATGAAGATG AGGATAATAA GGATTCGAAA
    GTGGAAAGTA CTGCTAAATT CATTCAAAAA ATGATCGCTT CTGCCTTGGA CTTGTGTAGT
    GTAACACTAA ACAAACTGTG CAGAGAACAC CGATATGTTG GATTTGTTTT GAGCAAGGAA
    AAACAAAAGT TGAAGTCAGG ACACTCGGAA TCTCTTTCAA ATACATCTAG AAAGCTGACG
    GATATTCGAT CTGCAGTTGA TCTACCAAGT CTTCAACTTG TGCAATCGGC GAATGATGTT
    GAAAAGATGG AGACAGCCGT CAGCGTGGAT TGGCAACAAA AATCATCAGC AACGAGACTA
    TTGAATGCAG TTGTGAATTG TATTGGAGCA CACACTGATA TTCTCTGCTA CTTCTTCGCA
    ATTATGACAC AAGTGATGAC TGGTGGACTT ATTACTCTTC CACTTCCTCT GATGTCACTA
    TTCTGGGGAA ATCTATCGAA TCCTCGTCCA TCCAAGTTCT TCTGGGTCAC AATGATCACC
    TACACTGAGT GTGTCATTGT TATCAAGTTT GTTTGCCAAT TTGCATTCAT GCCTTACAAT
    TCTATCACGT GGCGAACAGA GCATCAAATG GACCCGATGT CATTGGATAA GTTATTCGGA
    GTATCACAGA GAGATAGTTT TGCACTTTGG GACATTGTTT TGCTCTTCTC ACTCTTCTTC
    CATCGGTATA TGCTCAGAAA GTTAGGGTTA TGGAAGGATG CCAATTTGAC GGACACGTTC
    ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA GGATCGGACA CAGGATCACC CAAGAAAATT
    GCTCAAGAGC CAAAGGTTGT TGTAACTCAG TCAGATACCC TTGAAGGTAC AAGTGGAGGA
    GAGATAGTCA TACCATCTGA TCCAAATGCA GTTTCCAATA TGGAAGAATT AGACTGCGAA
    CCTCCGATTC CAGAGAAGCA AAGTGGACCA ATTGGAAGAT TCATTCATCA ATTATTCCAT
    CCAAAATTCC GTTACATTCG TGATTTGTAT CCAATAATGT TTGGAATCGA TGTGATATGC
    TTCCTCATCA TGACATTTGG ATACTCGGCA TTTGGTGAAG GAGGTTCTGG AAATGTTTTA
    GATGATGTGA AGGCTTCTCG TATTCCGGTA ACACTTGTTG TTATGCTCGT TGGAATGACT
    CTGGCAATTA TCATTGATCG AGCACTCTAC CTCAGAAAAT CAGTTGTCGG AAAGCTAATC
    TATCAAGTCT TAATGATTGC TTTCCTTCAC ATTTGGGTAT TCCTTGTTCT TCCAAATATG
    ACTCGTCGTT CTGCGATATC GAATCACGTT GCTCAAGCAT TGTACGTCAT CAAATCGTGC
    TATTTCCTTG TGTCTGCTTG GCAGATCCGA AATGGTTATC CTGAACTTTG TATTGGAAAT
    CTATTAACAC ATTCATACGG AATGACCAAT ATGATTGCAT TCAAAGTATT CATGAATATT
    CCATTCCTCT TCGAGCTTCG TACAGCTATT GATTGGACAT GGACAGATAC TTCAATGCCT
    CTTTTCGATT TCTTCAATAT GGAAAATTTC TATGCTCATA TATTCAATAT CAAGTGTGCC
    CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA AGAGGGATTC CCAAAGGAAA GTTGGTCAAG
    TATATGATGG GATTCCCAAT TATCATTGGA GTCGTTATCT TCATCTTTTC TCCTCTATTG
    CTCTGGTCGC TACTCAATCA AATCGGAACT ATCAGTATGC CAGAAAAAGT TACGTTGAGG
    ATTTCGATTG AAGGATATCC ACCACTTTAC GAGATGGAGG CACAAGGAAG TAATCACGAT
    AACGCTGAAC TTGGAATGAT AAAACCTGAT CAATTGGCAT CTTTGAATCA AGCATTGACG
    GATAGTTACA CGACACGAGA CACTAATTCT ATTTTAAGAT CAAGAATGTC TGTATCATAT
    CTGAAAGGAT ACACATATGA GGATATTCTG ATTGTGCGAT TCCGTCCAGA ATCTGAAATT
    TATTGGCCGA TTTCACAGGA TTCGAGAAAT GCGATGATCG ATAAATTGAG CCGAAACACG
    TCAGTCAATT TTGAAGTATC TCTGGAATTC ACTCGGCCAT ATGATCCAAA TGAAAACGCT
    GCTTTAAAAC ATTCGAAATC GTGGCTTGTT CCAATCTCCC TGGATATGAC GATTCGAGCA
    AAAATTCAAA GTGCTCTCCG AGGAGATCCA GGTCATCCGA TTCTCATTCC ACAGTCAATT
    CCTGCGTTTA TTCAAGTTCC AAATCAAGGA GAATTGACCC TTCCAACATC AATCGGAAAT
    ACTATTATCA ATGACGGAAA TCCACGGATT AATACGACCG GGATGGAAAA ATCAGACGAA
    GCTCGGGCTT GGTTCGACTC GTTAACCTTG AATCTCGAAC AAGGAAAATC GCAAAACGAG
    AAAATGTGGA TCGCCACATC CGAACATCCA GGAGACCAAA ATGCGAAACT CTGGATAAAG
    ACTGCAAATA CGACGTATTC GGGAAGGCCG TACCTTCAAG TTGTCGGATT TATTGATAGA
    GCATTCCCAT CATTTTTGGC GAAAGTTTTC AAAGGAGGAG TCATTGCAGT GTATCTTTCA
    GTTATCCTCG TCGTTGGAAG AGGTCTGGTT CGTGGAATCT TCACCACATC TCCAAGCACT
    GTCATGTTCA CAGAATTACC GAATGCGGAT CATCTTCTCA AAATTTGTCT CGACATCTAT
    TTGGTTCGAG AAGCGAAGGA CTTCATGTTG GAGCAGGATC TCTTCGCCAA ATTAATATTC
    CTGTTCCGAT CACCAGCGAC ACTCATCGAA TGGACACGAA TGAGCAAAAA GAAGCAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255390.2
    CDS1..7203
    Translation

    Target ORF information:

    RefSeq Version NM_001268461.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268461.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGGATCCAAA AAGTCGGAAG
    TTCGCGGCAT TTGTGGAATA TCTGTCGAAT AAAGTGTCTG TCTACTTCAT ATTTGTAGTA
    TCAGTGGTAC TTTTGGTGGT CTCTACGTGT TTTGCTCCGA ATTTCTACAA TATTCTATTC
    TTCGCACTAT GGGCATTGAA CTTGATTTAT CTCAAGTTCT CATTCCGTCT GTATCGTGGA
    TTGGCATATG CATTCTGGCT AACTCTCACG TTCTACACTT CAATTGTTAT TATCGCCCTC
    TACATTTATC AGTTCCCTGG AGTATCTCAA TGGATTATTC GAAATACTAG TTTGTCGCAG
    GAGTGGCTGA ATGCAATTGG ACTTGTCGAT TTTAGAGCTA TCGGTGAATC GGGAGCCCTA
    TTTCTACAAT TACTCGCCCC AATTGCCTTG TTCGTCGTCA CAATGCTCCA ACTCAAGTTT
    TTCCATGGAC CATGGAGTCG AGCAACGTCA CCAAGAAGAG CAGAAAATGA TCCACCTACA
    AGTACGACGG AGGCTGCGGC GGTAGCAAGC ACATCTGGAA CACAAGGAAG AGCACATGCT
    GCAGGCGATA CCCTTGTCAA GAAGCTGCAC AAATTGGCAA ATCAGACAAT CGAACTGTTA
    TGGAGGTTTT TCGAAGTTCA CATTTCGAAA ATTGTCTTTG TCATCATTGC GATTTTTATT
    GCCAACAATA TCAATGCTCT ATACATTCCA CTTGTTATTC TCCTCTCATT GGCTATATGT
    CTACCATCAG CTGCAGATGG GATATTCTCT TTATTTATGT GTGCCTACTT ATTCCTCGTG
    GCACTGAGCA AGATGATCTA CCAACTGGAT ATTGTTCCCG AGTTATCACA AATCGACCGA
    GGAGTTGGAG CAGATAACTG TTCACATGGA AATATTTCAA TGCCTGAATG GTTTGGATTG
    AAGAAGGAAG TTGAAGGAAC TGAACCAATT TATATGCTCT TTGGAGTCAT TGTTTCGATT
    ATTGCATTGG CTTTTCAATC AATTGTTATA TATCGACAAA GACATTATCG AGCTTCACTG
    GGACTTCCTG AATCGATGCG AGCTAAAGTG TTCCCGGACT TCCATCATTC TCATTTTGAT
    AGATCTCTCA AAAATGCCAT CCAATTCTTG ATAGACTATG GTTTCTATAA GTTTGGACTT
    GAAATCACGA TGATCGCCAT TGGAATTGAC ATTTTCAATC GAATGGATGC ATTGGCAGCG
    ATTCAATGTT TCTGGCTTGT CCTGTTTGCG CTCAATAAAA GAGTCTTTGT TCGAAGAATA
    TGGGTATTCT ATGTGATCTA TATGGCGATT CTGTATCCAC TTCAGTTCTT TTCATATGTT
    GGATTACCAC CTGACAGTTG TATTGAGTAT CCATGGTCGT ATTGGATTCC ATCGTATAGT
    GATGATGCGA GATTCAATCT GTCGTATCTT CTGAACTTGT CAATATACGG TGTAAACTGG
    CCATCAGCAT ATCTCATTGG CGATTTCTTC GTACTCCTCT TGGCATCATG CCAATTAGCA
    GTTTTCCGGC GAGAAGGAGA AGATAATGAT TCAATTTACA ATGATGGAAA CTTTGTGATT
    AAGCCAGAAA ATCCACAATA TGACTTTATT GACACCAAAA AGAGTTACGT TGATTATTTC
    AAATCATTCG TTTTCCATTA TGGACACTGG ATTACATTGA TGTCTACACT AGCAGCAGGA
    ATTGCTGGAA CGTCACTTTT TGCTTTGGGA TACATTATAT TCACTCTGAC AATGCTGTGG
    TCTGGTAACA ATTTGTATGT GATGAACTCA ACTCTACGAT CATTTGAACA TACTTTGAAA
    CGATGGAATG CACTTTTGGG ATACACATTG TTCACGATTA CAATGAAAGT TTGCCTTCAA
    ATATTTGGAT GTGTATTCCT GTCGTGGTTC GATCAATCTG GTGGATGGGG AAAGACTCTG
    TGCATCGTTC GTCAACTCTT CAGTATCACA TGTGTTAATA ACGAATGTCA TGTGCTCAAA
    GAGCTGGAGG ATTTCTCGAA GGCGTGTGCT GTTGAGACGA AAGAAGGAAA TATTGGATTT
    GATGTGATTG CGCTATCCTT CCTCGTCTTC CAAATCCGAA TTTTCCATTC TTGGTATTTC
    CAACACTGCA TGGTTGAATA TCGAAGTGAG GTTATTCTGG CCAATAGAGG TGCCGTGCTG
    AAGAATCAAC TGATTGAAAA GGAAATGAAG GAACAAAACG AGCAGCAAAA AGCCAAGTTC
    AATGATATCA GAAGAAGAAC TGAAGCTATT CGTGAAAGAT ATCAAAAACA AATCGAACGA
    GGAGCTGCTG AACGTGACTT CGAGCCGGTT ACTTATGGGC ACGCCAAACG AGCGGGAGAC
    TACTACATGT TCAAATACGA TCCTGAAAAT GATGATCTTG TGGAACCCGT CGACAGTTTT
    GTACCAGAAG TTGATCCCAA GGCAACTGCA TATGATCGAC TTGATCCTGG ACAGATAATG
    TACGCTGCCA CCGCTCACGA CCTCGACCTC GCCAAAACTG TACAGCAAGT CAAGAAAGGC
    GATACTATCA AAGATCCTGA TTCACGTGCT CTTATCGCTG TTTCTGAACC AGAAGCTCGT
    AAGCCAGGAG GCACTGAAGA AACGGATGGT GATGAAGATG AGGATAATAA GGATTCGAAA
    GTGGAAAGTA CTGCTAAATT CATTCAAAAA ATGATCGCTT CTGCCTTGGA CTTGTGTAGT
    GTAACACTAA ACAAACTGTG CAGAGAACAC CGATATGTTG GATTTGTTTT GAGCAAGGAA
    AAACAAAAGT TGAAGTCAGG ACACTCGGAA TCTCTTTCAA ATACATCTAG AAAGCTGACG
    GATATTCGAT CTGCAGTTGA TCTACCAAGT CTTCAACTTG TGCAATCGGC GAATGATGTT
    GAAAAGATGG AGACAGCCGT CAGCGTGGAT TGGCAACAAA AATCATCAGC AACGAGACTA
    TTGAATGCAG TTGTGAATTG TATTGGAGCA CACACTGATA TTCTCTGCTA CTTCTTCGCA
    ATTATGACAC AAGTGATGAC TGGTGGACTT ATTACTCTTC CACTTCCTCT GATGTCACTA
    TTCTGGGGAA ATCTATCGAA TCCTCGTCCA TCCAAGTTCT TCTGGGTCAC AATGATCACC
    TACACTGAGT GTGTCATTGT TATCAAGTTT GTTTGCCAAT TTGCATTCAT GCCTTACAAT
    TCTATCACGT GGCGAACAGA GCATCAAATG GACCCGATGT CATTGGATAA GTTATTCGGA
    GTATCACAGA GAGATAGTTT TGCACTTTGG GACATTGTTT TGCTCTTCTC ACTCTTCTTC
    CATCGGTATA TGCTCAGAAA GTTAGGGTTA TGGAAGGATG CCAATTTGAC GGACACGTTC
    ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA GGATCGGACA CAGGATCACC CAAGAAAATT
    GCTCAAGAGC CAAAGGTTGT TGTAACTCAG TCAGATACCC TTGAAGGTAC AAGTGGAGGA
    GAGATAGTCA TACCATCTGA TCCAAATGCA GTTTCCAATA TGGAAGAATT AGACTGCGAA
    CCTCCGATTC CAGAGAAGCA AAGTGGACCA ATTGGAAGAT TCATTCATCA ATTATTCCAT
    CCAAAATTCC GTTACATTCG TGATTTGTAT CCAATAATGT TTGGAATCGA TGTGATATGC
    TTCCTCATCA TGACATTTGG ATACTCGGCA TTTGGTGAAG GAGGTTCTGG AAATGTTTTA
    GATGATGTGA AGGCTTCTCG TATTCCGGTA ACACTTGTTG TTATGCTCGT TGGAATGACT
    CTGGCAATTA TCATTGATCG AGCACTCTAC CTCAGAAAAT CAGTTGTCGG AAAGCTAATC
    TATCAAGTCT TAATGATTGC TTTCCTTCAC ATTTGGGTAT TCCTTGTTCT TCCAAATATG
    ACTCGTCGTT CTGCGATATC GAATCACGTT GCTCAAGCAT TGTACGTCAT CAAATCGTGC
    TATTTCCTTG TGTCTGCTTG GCAGATCCGA AATGGTTATC CTGAACTTTG TATTGGAAAT
    CTATTAACAC ATTCATACGG AATGACCAAT ATGATTGCAT TCAAAGTATT CATGAATATT
    CCATTCCTCT TCGAGCTTCG TACAGCTATT GATTGGACAT GGACAGATAC TTCAATGCCT
    CTTTTCGATT TCTTCAATAT GGAAAATTTC TATGCTCATA TATTCAATAT CAAGTGTGCC
    CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA AGAGGGATTC CCAAAGGAAA GTTGGTCAAG
    TATATGATGG GATTCCCAAT TATCATTGGA GTCGTTATCT TCATCTTTTC TCCTCTATTG
    CTCTGGTCGC TACTCAATCA AATCGGAACT ATCAGTATGC CAGAAAAAGT TACGTTGAGG
    ATTTCGATTG AAGGATATCC ACCACTTTAC GAGATGGAGG CACAAGGAAG TAATCACGAT
    AACGCTGAAC TTGGAATGAT AAAACCTGAT CAATTGGCAT CTTTGAATCA AGCATTGACG
    GATAGTTACA CGACACGAGA CACTAATTCT ATTTTAAGAT CAAGAATGTC TGTATCATAT
    CTGAAAGGAT ACACATATGA GGATATTCTG ATTGTGCGAT TCCGTCCAGA ATCTGAAATT
    TATTGGCCGA TTTCACAGGA TTCGAGAAAT GCGATGATCG ATAAATTGAG CCGAAACACG
    TCAGTCAATT TTGAAGTATC TCTGGAATTC ACTCGGCCAT ATGATCCAAA TGAAAACGCT
    GCTTTAAAAC ATTCGAAATC GTGGCTTGTT CCAATCTCCC TGGATATGAC GATTCGAGCA
    AAAATTCAAA GTGCTCTCCG AGGAGATCCA GGTCATCCGA TTCTCATTCC ACAGTCAATT
    CCTGCGTTTA TTCAAGTTCC AAATCAAGGA GAATTGACCC TTCCAACATC AATCGGAAAT
    ACTATTATCA ATGACGGAAA TCCACGGATT AATACGACCG GGATGGAAAA ATCAGACGAA
    GCTCGGGCTT GGTTCGACTC GTTAACCTTG AATCTCGAAC AAGGAAAATC GCAAAACGAG
    AAAATGTGGA TCGCCACATC CGAACATCCA GGAGACCAAA ATGCGAAACT CTGGATAAAG
    ACTGCAAATA CGACGTATTC GGGAAGGCCG TACCTTCAAG TTGTCGGATT TATTGATAGA
    GCATTCCCAT CATTTTTGGC GAAAGTTTTC AAAGGAGGAG TCATTGCAGT GTATCTTTCA
    GTTATCCTCG TCGTTGGAAG AGGTCTGGTT CGTGGAATCT TCACCACATC TCCAAGCACT
    GTCATGTTCA CAGAATTACC GAATGCGGAT CATCTTCTCA AAATTTGTCT CGACATCTAT
    TTGGTTCGAG AAGCGAAGGA CTTCATGTTG GAGCAGGATC TCTTCGCCAA ATTAATATTC
    CTGTTCCGAT CACCAGCGAC ACTCATCGAA TGGACACGAA TGAGCAAAAA GAAGCAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342181
    Clone ID Related Accession (Same CDS sequence) NM_001268461.2 , NM_001268461.3
    Accession Version NM_001268461.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268461.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGGATCCAAA AAGTCGGAAG
    TTCGCGGCAT TTGTGGAATA TCTGTCGAAT AAAGTGTCTG TCTACTTCAT ATTTGTAGTA
    TCAGTGGTAC TTTTGGTGGT CTCTACGTGT TTTGCTCCGA ATTTCTACAA TATTCTATTC
    TTCGCACTAT GGGCATTGAA CTTGATTTAT CTCAAGTTCT CATTCCGTCT GTATCGTGGA
    TTGGCATATG CATTCTGGCT AACTCTCACG TTCTACACTT CAATTGTTAT TATCGCCCTC
    TACATTTATC AGTTCCCTGG AGTATCTCAA TGGATTATTC GAAATACTAG TTTGTCGCAG
    GAGTGGCTGA ATGCAATTGG ACTTGTCGAT TTTAGAGCTA TCGGTGAATC GGGAGCCCTA
    TTTCTACAAT TACTCGCCCC AATTGCCTTG TTCGTCGTCA CAATGCTCCA ACTCAAGTTT
    TTCCATGGAC CATGGAGTCG AGCAACGTCA CCAAGAAGAG CAGAAAATGA TCCACCTACA
    AGTACGACGG AGGCTGCGGC GGTAGCAAGC ACATCTGGAA CACAAGGAAG AGCACATGCT
    GCAGGCGATA CCCTTGTCAA GAAGCTGCAC AAATTGGCAA ATCAGACAAT CGAACTGTTA
    TGGAGGTTTT TCGAAGTTCA CATTTCGAAA ATTGTCTTTG TCATCATTGC GATTTTTATT
    GCCAACAATA TCAATGCTCT ATACATTCCA CTTGTTATTC TCCTCTCATT GGCTATATGT
    CTACCATCAG CTGCAGATGG GATATTCTCT TTATTTATGT GTGCCTACTT ATTCCTCGTG
    GCACTGAGCA AGATGATCTA CCAACTGGAT ATTGTTCCCG AGTTATCACA AATCGACCGA
    GGAGTTGGAG CAGATAACTG TTCACATGGA AATATTTCAA TGCCTGAATG GTTTGGATTG
    AAGAAGGAAG TTGAAGGAAC TGAACCAATT TATATGCTCT TTGGAGTCAT TGTTTCGATT
    ATTGCATTGG CTTTTCAATC AATTGTTATA TATCGACAAA GACATTATCG AGCTTCACTG
    GGACTTCCTG AATCGATGCG AGCTAAAGTG TTCCCGGACT TCCATCATTC TCATTTTGAT
    AGATCTCTCA AAAATGCCAT CCAATTCTTG ATAGACTATG GTTTCTATAA GTTTGGACTT
    GAAATCACGA TGATCGCCAT TGGAATTGAC ATTTTCAATC GAATGGATGC ATTGGCAGCG
    ATTCAATGTT TCTGGCTTGT CCTGTTTGCG CTCAATAAAA GAGTCTTTGT TCGAAGAATA
    TGGGTATTCT ATGTGATCTA TATGGCGATT CTGTATCCAC TTCAGTTCTT TTCATATGTT
    GGATTACCAC CTGACAGTTG TATTGAGTAT CCATGGTCGT ATTGGATTCC ATCGTATAGT
    GATGATGCGA GATTCAATCT GTCGTATCTT CTGAACTTGT CAATATACGG TGTAAACTGG
    CCATCAGCAT ATCTCATTGG CGATTTCTTC GTACTCCTCT TGGCATCATG CCAATTAGCA
    GTTTTCCGGC GAGAAGGAGA AGATAATGAT TCAATTTACA ATGATGGAAA CTTTGTGATT
    AAGCCAGAAA ATCCACAATA TGACTTTATT GACACCAAAA AGAGTTACGT TGATTATTTC
    AAATCATTCG TTTTCCATTA TGGACACTGG ATTACATTGA TGTCTACACT AGCAGCAGGA
    ATTGCTGGAA CGTCACTTTT TGCTTTGGGA TACATTATAT TCACTCTGAC AATGCTGTGG
    TCTGGTAACA ATTTGTATGT GATGAACTCA ACTCTACGAT CATTTGAACA TACTTTGAAA
    CGATGGAATG CACTTTTGGG ATACACATTG TTCACGATTA CAATGAAAGT TTGCCTTCAA
    ATATTTGGAT GTGTATTCCT GTCGTGGTTC GATCAATCTG GTGGATGGGG AAAGACTCTG
    TGCATCGTTC GTCAACTCTT CAGTATCACA TGTGTTAATA ACGAATGTCA TGTGCTCAAA
    GAGCTGGAGG ATTTCTCGAA GGCGTGTGCT GTTGAGACGA AAGAAGGAAA TATTGGATTT
    GATGTGATTG CGCTATCCTT CCTCGTCTTC CAAATCCGAA TTTTCCATTC TTGGTATTTC
    CAACACTGCA TGGTTGAATA TCGAAGTGAG GTTATTCTGG CCAATAGAGG TGCCGTGCTG
    AAGAATCAAC TGATTGAAAA GGAAATGAAG GAACAAAACG AGCAGCAAAA AGCCAAGTTC
    AATGATATCA GAAGAAGAAC TGAAGCTATT CGTGAAAGAT ATCAAAAACA AATCGAACGA
    GGAGCTGCTG AACGTGACTT CGAGCCGGTT ACTTATGGGC ACGCCAAACG AGCGGGAGAC
    TACTACATGT TCAAATACGA TCCTGAAAAT GATGATCTTG TGGAACCCGT CGACAGTTTT
    GTACCAGAAG TTGATCCCAA GGCAACTGCA TATGATCGAC TTGATCCTGG ACAGATAATG
    TACGCTGCCA CCGCTCACGA CCTCGACCTC GCCAAAACTG TACAGCAAGT CAAGAAAGGC
    GATACTATCA AAGATCCTGA TTCACGTGCT CTTATCGCTG TTTCTGAACC AGAAGCTCGT
    AAGCCAGGAG GCACTGAAGA AACGGATGGT GATGAAGATG AGGATAATAA GGATTCGAAA
    GTGGAAAGTA CTGCTAAATT CATTCAAAAA ATGATCGCTT CTGCCTTGGA CTTGTGTAGT
    GTAACACTAA ACAAACTGTG CAGAGAACAC CGATATGTTG GATTTGTTTT GAGCAAGGAA
    AAACAAAAGT TGAAGTCAGG ACACTCGGAA TCTCTTTCAA ATACATCTAG AAAGCTGACG
    GATATTCGAT CTGCAGTTGA TCTACCAAGT CTTCAACTTG TGCAATCGGC GAATGATGTT
    GAAAAGATGG AGACAGCCGT CAGCGTGGAT TGGCAACAAA AATCATCAGC AACGAGACTA
    TTGAATGCAG TTGTGAATTG TATTGGAGCA CACACTGATA TTCTCTGCTA CTTCTTCGCA
    ATTATGACAC AAGTGATGAC TGGTGGACTT ATTACTCTTC CACTTCCTCT GATGTCACTA
    TTCTGGGGAA ATCTATCGAA TCCTCGTCCA TCCAAGTTCT TCTGGGTCAC AATGATCACC
    TACACTGAGT GTGTCATTGT TATCAAGTTT GTTTGCCAAT TTGCATTCAT GCCTTACAAT
    TCTATCACGT GGCGAACAGA GCATCAAATG GACCCGATGT CATTGGATAA GTTATTCGGA
    GTATCACAGA GAGATAGTTT TGCACTTTGG GACATTGTTT TGCTCTTCTC ACTCTTCTTC
    CATCGGTATA TGCTCAGAAA GTTAGGGTTA TGGAAGGATG CCAATTTGAC GGACACGTTC
    ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA GGATCGGACA CAGGATCACC CAAGAAAATT
    GCTCAAGAGC CAAAGGTTGT TGTAACTCAG TCAGATACCC TTGAAGGTAC AAGTGGAGGA
    GAGATAGTCA TACCATCTGA TCCAAATGCA GTTTCCAATA TGGAAGAATT AGACTGCGAA
    CCTCCGATTC CAGAGAAGCA AAGTGGACCA ATTGGAAGAT TCATTCATCA ATTATTCCAT
    CCAAAATTCC GTTACATTCG TGATTTGTAT CCAATAATGT TTGGAATCGA TGTGATATGC
    TTCCTCATCA TGACATTTGG ATACTCGGCA TTTGGTGAAG GAGGTTCTGG AAATGTTTTA
    GATGATGTGA AGGCTTCTCG TATTCCGGTA ACACTTGTTG TTATGCTCGT TGGAATGACT
    CTGGCAATTA TCATTGATCG AGCACTCTAC CTCAGAAAAT CAGTTGTCGG AAAGCTAATC
    TATCAAGTCT TAATGATTGC TTTCCTTCAC ATTTGGGTAT TCCTTGTTCT TCCAAATATG
    ACTCGTCGTT CTGCGATATC GAATCACGTT GCTCAAGCAT TGTACGTCAT CAAATCGTGC
    TATTTCCTTG TGTCTGCTTG GCAGATCCGA AATGGTTATC CTGAACTTTG TATTGGAAAT
    CTATTAACAC ATTCATACGG AATGACCAAT ATGATTGCAT TCAAAGTATT CATGAATATT
    CCATTCCTCT TCGAGCTTCG TACAGCTATT GATTGGACAT GGACAGATAC TTCAATGCCT
    CTTTTCGATT TCTTCAATAT GGAAAATTTC TATGCTCATA TATTCAATAT CAAGTGTGCC
    CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA AGAGGGATTC CCAAAGGAAA GTTGGTCAAG
    TATATGATGG GATTCCCAAT TATCATTGGA GTCGTTATCT TCATCTTTTC TCCTCTATTG
    CTCTGGTCGC TACTCAATCA AATCGGAACT ATCAGTATGC CAGAAAAAGT TACGTTGAGG
    ATTTCGATTG AAGGATATCC ACCACTTTAC GAGATGGAGG CACAAGGAAG TAATCACGAT
    AACGCTGAAC TTGGAATGAT AAAACCTGAT CAATTGGCAT CTTTGAATCA AGCATTGACG
    GATAGTTACA CGACACGAGA CACTAATTCT ATTTTAAGAT CAAGAATGTC TGTATCATAT
    CTGAAAGGAT ACACATATGA GGATATTCTG ATTGTGCGAT TCCGTCCAGA ATCTGAAATT
    TATTGGCCGA TTTCACAGGA TTCGAGAAAT GCGATGATCG ATAAATTGAG CCGAAACACG
    TCAGTCAATT TTGAAGTATC TCTGGAATTC ACTCGGCCAT ATGATCCAAA TGAAAACGCT
    GCTTTAAAAC ATTCGAAATC GTGGCTTGTT CCAATCTCCC TGGATATGAC GATTCGAGCA
    AAAATTCAAA GTGCTCTCCG AGGAGATCCA GGTCATCCGA TTCTCATTCC ACAGTCAATT
    CCTGCGTTTA TTCAAGTTCC AAATCAAGGA GAATTGACCC TTCCAACATC AATCGGAAAT
    ACTATTATCA ATGACGGAAA TCCACGGATT AATACGACCG GGATGGAAAA ATCAGACGAA
    GCTCGGGCTT GGTTCGACTC GTTAACCTTG AATCTCGAAC AAGGAAAATC GCAAAACGAG
    AAAATGTGGA TCGCCACATC CGAACATCCA GGAGACCAAA ATGCGAAACT CTGGATAAAG
    ACTGCAAATA CGACGTATTC GGGAAGGCCG TACCTTCAAG TTGTCGGATT TATTGATAGA
    GCATTCCCAT CATTTTTGGC GAAAGTTTTC AAAGGAGGAG TCATTGCAGT GTATCTTTCA
    GTTATCCTCG TCGTTGGAAG AGGTCTGGTT CGTGGAATCT TCACCACATC TCCAAGCACT
    GTCATGTTCA CAGAATTACC GAATGCGGAT CATCTTCTCA AAATTTGTCT CGACATCTAT
    TTGGTTCGAG AAGCGAAGGA CTTCATGTTG GAGCAGGATC TCTTCGCCAA ATTAATATTC
    CTGTTCCGAT CACCAGCGAC ACTCATCGAA TGGACACGAA TGAGCAAAAA GAAGCAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255390.2
    CDS58..7260
    Translation

    Target ORF information:

    RefSeq Version NM_001268461.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268461.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGGATCCAAA AAGTCGGAAG
    TTCGCGGCAT TTGTGGAATA TCTGTCGAAT AAAGTGTCTG TCTACTTCAT ATTTGTAGTA
    TCAGTGGTAC TTTTGGTGGT CTCTACGTGT TTTGCTCCGA ATTTCTACAA TATTCTATTC
    TTCGCACTAT GGGCATTGAA CTTGATTTAT CTCAAGTTCT CATTCCGTCT GTATCGTGGA
    TTGGCATATG CATTCTGGCT AACTCTCACG TTCTACACTT CAATTGTTAT TATCGCCCTC
    TACATTTATC AGTTCCCTGG AGTATCTCAA TGGATTATTC GAAATACTAG TTTGTCGCAG
    GAGTGGCTGA ATGCAATTGG ACTTGTCGAT TTTAGAGCTA TCGGTGAATC GGGAGCCCTA
    TTTCTACAAT TACTCGCCCC AATTGCCTTG TTCGTCGTCA CAATGCTCCA ACTCAAGTTT
    TTCCATGGAC CATGGAGTCG AGCAACGTCA CCAAGAAGAG CAGAAAATGA TCCACCTACA
    AGTACGACGG AGGCTGCGGC GGTAGCAAGC ACATCTGGAA CACAAGGAAG AGCACATGCT
    GCAGGCGATA CCCTTGTCAA GAAGCTGCAC AAATTGGCAA ATCAGACAAT CGAACTGTTA
    TGGAGGTTTT TCGAAGTTCA CATTTCGAAA ATTGTCTTTG TCATCATTGC GATTTTTATT
    GCCAACAATA TCAATGCTCT ATACATTCCA CTTGTTATTC TCCTCTCATT GGCTATATGT
    CTACCATCAG CTGCAGATGG GATATTCTCT TTATTTATGT GTGCCTACTT ATTCCTCGTG
    GCACTGAGCA AGATGATCTA CCAACTGGAT ATTGTTCCCG AGTTATCACA AATCGACCGA
    GGAGTTGGAG CAGATAACTG TTCACATGGA AATATTTCAA TGCCTGAATG GTTTGGATTG
    AAGAAGGAAG TTGAAGGAAC TGAACCAATT TATATGCTCT TTGGAGTCAT TGTTTCGATT
    ATTGCATTGG CTTTTCAATC AATTGTTATA TATCGACAAA GACATTATCG AGCTTCACTG
    GGACTTCCTG AATCGATGCG AGCTAAAGTG TTCCCGGACT TCCATCATTC TCATTTTGAT
    AGATCTCTCA AAAATGCCAT CCAATTCTTG ATAGACTATG GTTTCTATAA GTTTGGACTT
    GAAATCACGA TGATCGCCAT TGGAATTGAC ATTTTCAATC GAATGGATGC ATTGGCAGCG
    ATTCAATGTT TCTGGCTTGT CCTGTTTGCG CTCAATAAAA GAGTCTTTGT TCGAAGAATA
    TGGGTATTCT ATGTGATCTA TATGGCGATT CTGTATCCAC TTCAGTTCTT TTCATATGTT
    GGATTACCAC CTGACAGTTG TATTGAGTAT CCATGGTCGT ATTGGATTCC ATCGTATAGT
    GATGATGCGA GATTCAATCT GTCGTATCTT CTGAACTTGT CAATATACGG TGTAAACTGG
    CCATCAGCAT ATCTCATTGG CGATTTCTTC GTACTCCTCT TGGCATCATG CCAATTAGCA
    GTTTTCCGGC GAGAAGGAGA AGATAATGAT TCAATTTACA ATGATGGAAA CTTTGTGATT
    AAGCCAGAAA ATCCACAATA TGACTTTATT GACACCAAAA AGAGTTACGT TGATTATTTC
    AAATCATTCG TTTTCCATTA TGGACACTGG ATTACATTGA TGTCTACACT AGCAGCAGGA
    ATTGCTGGAA CGTCACTTTT TGCTTTGGGA TACATTATAT TCACTCTGAC AATGCTGTGG
    TCTGGTAACA ATTTGTATGT GATGAACTCA ACTCTACGAT CATTTGAACA TACTTTGAAA
    CGATGGAATG CACTTTTGGG ATACACATTG TTCACGATTA CAATGAAAGT TTGCCTTCAA
    ATATTTGGAT GTGTATTCCT GTCGTGGTTC GATCAATCTG GTGGATGGGG AAAGACTCTG
    TGCATCGTTC GTCAACTCTT CAGTATCACA TGTGTTAATA ACGAATGTCA TGTGCTCAAA
    GAGCTGGAGG ATTTCTCGAA GGCGTGTGCT GTTGAGACGA AAGAAGGAAA TATTGGATTT
    GATGTGATTG CGCTATCCTT CCTCGTCTTC CAAATCCGAA TTTTCCATTC TTGGTATTTC
    CAACACTGCA TGGTTGAATA TCGAAGTGAG GTTATTCTGG CCAATAGAGG TGCCGTGCTG
    AAGAATCAAC TGATTGAAAA GGAAATGAAG GAACAAAACG AGCAGCAAAA AGCCAAGTTC
    AATGATATCA GAAGAAGAAC TGAAGCTATT CGTGAAAGAT ATCAAAAACA AATCGAACGA
    GGAGCTGCTG AACGTGACTT CGAGCCGGTT ACTTATGGGC ACGCCAAACG AGCGGGAGAC
    TACTACATGT TCAAATACGA TCCTGAAAAT GATGATCTTG TGGAACCCGT CGACAGTTTT
    GTACCAGAAG TTGATCCCAA GGCAACTGCA TATGATCGAC TTGATCCTGG ACAGATAATG
    TACGCTGCCA CCGCTCACGA CCTCGACCTC GCCAAAACTG TACAGCAAGT CAAGAAAGGC
    GATACTATCA AAGATCCTGA TTCACGTGCT CTTATCGCTG TTTCTGAACC AGAAGCTCGT
    AAGCCAGGAG GCACTGAAGA AACGGATGGT GATGAAGATG AGGATAATAA GGATTCGAAA
    GTGGAAAGTA CTGCTAAATT CATTCAAAAA ATGATCGCTT CTGCCTTGGA CTTGTGTAGT
    GTAACACTAA ACAAACTGTG CAGAGAACAC CGATATGTTG GATTTGTTTT GAGCAAGGAA
    AAACAAAAGT TGAAGTCAGG ACACTCGGAA TCTCTTTCAA ATACATCTAG AAAGCTGACG
    GATATTCGAT CTGCAGTTGA TCTACCAAGT CTTCAACTTG TGCAATCGGC GAATGATGTT
    GAAAAGATGG AGACAGCCGT CAGCGTGGAT TGGCAACAAA AATCATCAGC AACGAGACTA
    TTGAATGCAG TTGTGAATTG TATTGGAGCA CACACTGATA TTCTCTGCTA CTTCTTCGCA
    ATTATGACAC AAGTGATGAC TGGTGGACTT ATTACTCTTC CACTTCCTCT GATGTCACTA
    TTCTGGGGAA ATCTATCGAA TCCTCGTCCA TCCAAGTTCT TCTGGGTCAC AATGATCACC
    TACACTGAGT GTGTCATTGT TATCAAGTTT GTTTGCCAAT TTGCATTCAT GCCTTACAAT
    TCTATCACGT GGCGAACAGA GCATCAAATG GACCCGATGT CATTGGATAA GTTATTCGGA
    GTATCACAGA GAGATAGTTT TGCACTTTGG GACATTGTTT TGCTCTTCTC ACTCTTCTTC
    CATCGGTATA TGCTCAGAAA GTTAGGGTTA TGGAAGGATG CCAATTTGAC GGACACGTTC
    ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA GGATCGGACA CAGGATCACC CAAGAAAATT
    GCTCAAGAGC CAAAGGTTGT TGTAACTCAG TCAGATACCC TTGAAGGTAC AAGTGGAGGA
    GAGATAGTCA TACCATCTGA TCCAAATGCA GTTTCCAATA TGGAAGAATT AGACTGCGAA
    CCTCCGATTC CAGAGAAGCA AAGTGGACCA ATTGGAAGAT TCATTCATCA ATTATTCCAT
    CCAAAATTCC GTTACATTCG TGATTTGTAT CCAATAATGT TTGGAATCGA TGTGATATGC
    TTCCTCATCA TGACATTTGG ATACTCGGCA TTTGGTGAAG GAGGTTCTGG AAATGTTTTA
    GATGATGTGA AGGCTTCTCG TATTCCGGTA ACACTTGTTG TTATGCTCGT TGGAATGACT
    CTGGCAATTA TCATTGATCG AGCACTCTAC CTCAGAAAAT CAGTTGTCGG AAAGCTAATC
    TATCAAGTCT TAATGATTGC TTTCCTTCAC ATTTGGGTAT TCCTTGTTCT TCCAAATATG
    ACTCGTCGTT CTGCGATATC GAATCACGTT GCTCAAGCAT TGTACGTCAT CAAATCGTGC
    TATTTCCTTG TGTCTGCTTG GCAGATCCGA AATGGTTATC CTGAACTTTG TATTGGAAAT
    CTATTAACAC ATTCATACGG AATGACCAAT ATGATTGCAT TCAAAGTATT CATGAATATT
    CCATTCCTCT TCGAGCTTCG TACAGCTATT GATTGGACAT GGACAGATAC TTCAATGCCT
    CTTTTCGATT TCTTCAATAT GGAAAATTTC TATGCTCATA TATTCAATAT CAAGTGTGCC
    CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA AGAGGGATTC CCAAAGGAAA GTTGGTCAAG
    TATATGATGG GATTCCCAAT TATCATTGGA GTCGTTATCT TCATCTTTTC TCCTCTATTG
    CTCTGGTCGC TACTCAATCA AATCGGAACT ATCAGTATGC CAGAAAAAGT TACGTTGAGG
    ATTTCGATTG AAGGATATCC ACCACTTTAC GAGATGGAGG CACAAGGAAG TAATCACGAT
    AACGCTGAAC TTGGAATGAT AAAACCTGAT CAATTGGCAT CTTTGAATCA AGCATTGACG
    GATAGTTACA CGACACGAGA CACTAATTCT ATTTTAAGAT CAAGAATGTC TGTATCATAT
    CTGAAAGGAT ACACATATGA GGATATTCTG ATTGTGCGAT TCCGTCCAGA ATCTGAAATT
    TATTGGCCGA TTTCACAGGA TTCGAGAAAT GCGATGATCG ATAAATTGAG CCGAAACACG
    TCAGTCAATT TTGAAGTATC TCTGGAATTC ACTCGGCCAT ATGATCCAAA TGAAAACGCT
    GCTTTAAAAC ATTCGAAATC GTGGCTTGTT CCAATCTCCC TGGATATGAC GATTCGAGCA
    AAAATTCAAA GTGCTCTCCG AGGAGATCCA GGTCATCCGA TTCTCATTCC ACAGTCAATT
    CCTGCGTTTA TTCAAGTTCC AAATCAAGGA GAATTGACCC TTCCAACATC AATCGGAAAT
    ACTATTATCA ATGACGGAAA TCCACGGATT AATACGACCG GGATGGAAAA ATCAGACGAA
    GCTCGGGCTT GGTTCGACTC GTTAACCTTG AATCTCGAAC AAGGAAAATC GCAAAACGAG
    AAAATGTGGA TCGCCACATC CGAACATCCA GGAGACCAAA ATGCGAAACT CTGGATAAAG
    ACTGCAAATA CGACGTATTC GGGAAGGCCG TACCTTCAAG TTGTCGGATT TATTGATAGA
    GCATTCCCAT CATTTTTGGC GAAAGTTTTC AAAGGAGGAG TCATTGCAGT GTATCTTTCA
    GTTATCCTCG TCGTTGGAAG AGGTCTGGTT CGTGGAATCT TCACCACATC TCCAAGCACT
    GTCATGTTCA CAGAATTACC GAATGCGGAT CATCTTCTCA AAATTTGTCT CGACATCTAT
    TTGGTTCGAG AAGCGAAGGA CTTCATGTTG GAGCAGGATC TCTTCGCCAA ATTAATATTC
    CTGTTCCGAT CACCAGCGAC ACTCATCGAA TGGACACGAA TGAGCAAAAA GAAGCAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342182
    Clone ID Related Accession (Same CDS sequence) NM_001268458.3 , NM_001268458.2
    Accession Version NM_001268458.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268458.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACTACAGCAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGGATCCAAA AAGTCGGAAG
    TTCGCGGCAT TTGTGGAATA TCTGTCGAAT AAAGTGTCTG TCTACTTCAT ATTTGTAGTA
    TCAGTGGTAC TTTTGGTGGT CTCTACGTGT TTTGCTCCGA ATTTCTACAA TATTCTATTC
    TTCGCACTAT GGGCATTGAA CTTGATTTAT CTCAAGTTCT CATTCCGTCT GTATCGTGGA
    TTGGCATATG CATTCTGGCT AACTCTCACG TTCTACACTT CAATTGTTAT TATCGCCCTC
    TACATTTATC AGTTCCCTGG AGTATCTCAA TGGATTATTC GAAATACTAG TTTGTCGCAG
    GAGTGGCTGA ATGCAATTGG ACTTGTCGAT TTTAGAGCTA TCGGTGAATC GGGAGCCCTA
    TTTCTACAAT TACTCGCCCC AATTGCCTTG TTCGTCGTCA CAATGCTCCA ACTCAAGTTT
    TTCCATGGAC CATGGAGTCG AGCAACGTCA CCAAGAAGAG CAGAAAATGA TCCACCTACA
    AGTACGACGG AGGCTGCGGC GGTAGCAAGC ACATCTGGAA CACAAGGAAG AGCACATGCT
    GCAGGCGATA CCCTTGTCAA GAAGCTGCAC AAATTGGCAA ATCAGACAAT CGAACTGTTA
    TGGAGGTTTT TCGAAGTTCA CATTTCGAAA ATTGTCTTTG TCATCATTGC GATTTTTATT
    GCCAACAATA TCAATGCTCT ATACATTCCA CTTGTTATTC TCCTCTCATT GGCTATATGT
    CTACCATCAG CTGCAGATGG GATATTCTCT TTATTTATGT GTGCCTACTT ATTCCTCGTG
    GCACTGAGCA AGATGATCTA CCAACTGGAT ATTGTTCCCG AGTTATCACA AATCGACCGA
    GGAGTTGGAG CAGATAACTG TTCACATGGA AATATTTCAA TGCCTGAATG GTTTGGATTG
    AAGAAGGAAG TTGAAGGAAC TGAACCAATT TATATGCTCT TTGGAGTCAT TGTTTCGATT
    ATTGCATTGG CTTTTCAATC AATTGTTATA TATCGACAAA GACATTATCG AGCTTCACTG
    GGACTTCCTG AATCGATGCG AGCTAAAGTG TTCCCGGACT TCCATCATTC TCATTTTGAT
    AGATCTCTCA AAAATGCCAT CCAATTCTTG ATAGACTATG GTTTCTATAA GTTTGGACTT
    GAAATCACGA TGATCGCCAT TGGAATTGAC ATTTTCAATC GAATGGATGC ATTGGCAGCG
    ATTCAATGTT TCTGGCTTGT CCTGTTTGCG CTCAATAAAA GAGTCTTTGT TCGAAGAATA
    TGGGTATTCT ATGTGATCTA TATGGCGATT CTGTATCCAC TTCAGTTCTT TTCATATGTT
    GGATTACCAC CTGACAGTTG TATTGAGTAT CCATGGTCGT ATTGGATTCC ATCGTATAGT
    GATGATGCGA GATTCAATCT GTCGTATCTT CTGAACTTGT CAATATACGG TGTAAACTGG
    CCATCAGCAT ATCTCATTGG CGATTTCTTC GTACTCCTCT TGGCATCATG CCAATTAGCA
    GTTTTCCGGC GAGAAGGAGA AGATAATGAT TCAATTTACA ATGATGGAAA CTTTGTGATT
    AAGCCAGAAA ATCCACAATA TGACTTTATT GACACCAAAA AGAGTTACGT TGATTATTTC
    AAATCATTCG TTTTCCATTA TGGACACTGG ATTACATTGA TGTCTACACT AGCAGCAGGA
    ATTGCTGGAA CGTCACTTTT TGCTTTGGGA TACATTATAT TCACTCTGAC AATGCTGTGG
    TCTGGTAACA ATTTGTATGT GATGAACTCA ACTCTACGAT CATTTGAACA TACTTTGAAA
    CGATGGAATG CACTTTTGGG ATACACATTG TTCACGATTA CAATGAAAGT TTGCCTTCAA
    ATATTTGGAT GTGTATTCCT GTCGTGGTTC GATCAATCTG GTGGATGGGG AAAGACTCTG
    TGCATCGTTC GTCAACTCTT CAGTATCACA TGTGTTAATA ACGAATGTCA TGTGCTCAAA
    GAGCTGGAGG ATTTCTCGAA GGCGTGTGCT GTTGAGACGA AAGAAGGAAA TATTGGATTT
    GATGTGATTG CGCTATCCTT CCTCGTCTTC CAAATCCGAA TTTTCCATTC TTGGTATTTC
    CAACACTGCA TGGTTGAATA TCGAAGTGAG GTTATTCTGG CCAATAGAGG TGCCGTGCTG
    AAGAATCAAC TGATTGAAAA GGAAATGAAG GAACAAAACG AGCAGCAAAA AGCCAAGTTC
    AATGATATCA GAAGAAGAAC TGAAGCTATT CGTGAAAGAT ATCAAAAACA AATCGAACGA
    GGAGCTGCTG AACGTGACTT CGAGCCGGTT ACTTATGGGC ACGCCAAACG AGCGGGAGAC
    TACTACATGT TCAAATACGA TCCTGAAAAT GATGATCTTG TGGAACCCGT CGACAGTTTT
    GTACCAGAAG TTGATCCCAA GGCAACTGCA TATGATCGAC TTGATCCTGG ACAGATAATG
    TACGCTGCCA CCGCTCACGA CCTCGACCTC GCCAAAACTG TACAGCAAGT CAAGAAAGGC
    GATACTATCA AAGATCCTGA TTCACGTGCT CTTATCGCTG TTTCTGAACC AGAAGCTCGT
    AAGCCAGGAG GCACTGAAGA AACGGATGGT GATGAAGATG AGGATAATAA GGATTCGAAA
    GTGGAAAGTA CTGCTAAATT CATTCAAAAA ATGATCGCTT CTGCCTTGGA CTTGTGTAGT
    GTAACACTAA ACAAACTGTG CAGAGAACAC CGATATGTTG GATTTGTTTT GAGCAAGGAA
    AAACAAAAGT TGAAGTCAGG ACACTCGGAA TCTCTTTCAA ATACATCTAG AAAGCTGACG
    GATATTCGAT CTGCAGTTGA TCTACCAAGT CTTCAACTTG TGCAATCGGC GAATGATGTT
    GAAAAGATGG AGACAGCCGT CAGCGTGGAT TGGCAACAAA AATCATCAGC AACGAGACTA
    TTGAATGCAG TTGTGAATTG TATTGGAGCA CACACTGATA TTCTCTGCTA CTTCTTCGCA
    ATTATGACAC AAGTGATGAC TGGTGGACTT ATTACTCTTC CACTTCCTCT GATGTCACTA
    TTCTGGGGAA ATCTATCGAA TCCTCGTCCA TCCAAGTTCT TCTGGGTCAC AATGATCACC
    TACACTGAGT GTGTCATTGT TATCAAGTTT GTTTGCCAAT TTGCATTCAT GCCTTACAAT
    TCTATCACGT GGCGAACAGA GCATCAAATG GACCCGATGT CATTGGATAA GTTATTCGGA
    GTATCACAGA GAGATAGTTT TGCACTTTGG GACATTGTTT TGCTCTTCTC ACTCTTCTTC
    CATCGGTATA TGCTCAGAAA GTTAGGGTTA TGGAAGGATG CCAATTTGAC GGACACGTTC
    ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA GGATCGGACA CAGGATCACC CAAGAAAATT
    GCTCAAGAGC CAAAGGTTGT TGTAACTCAG TCAGATACCC TTGAAGGTAC AAGTGGAGGA
    GAGATAGTCA TACCATCTGA TCCAAATGCA GTTTCCAATA TGGAAGAATT AGACTGCGAA
    CCTCCGATTC CAGAGAAGCA AAGTGGACCA ATTGGAAGAT TCATTCATCA ATTATTCCAT
    CCAAAATTCC GTTACATTCG TGATTTGTAT CCAATAATGT TTGGAATCGA TGTGATATGC
    TTCCTCATCA TGACATTTGG ATACTCGGCA TTTGGTGAAG GAGGTTCTGG AAATGTTTTA
    GATGATGTGA AGGCTTCTCG TATTCCGGTA ACACTTGTTG TTATGCTCGT TGGAATGACT
    CTGGCAATTA TCATTGATCG AGCACTCTAC CTCAGAAAAT CAGTTGTCGG AAAGCTAATC
    TATCAAGTCT TAATGATTGC TTTCCTTCAC ATTTGGGTAT TCCTTGTTCT TCCAAATATG
    ACTCGTCGTT CTGCGATATC GAATCACGTT GCTCAAGCAT TGTACGTCAT CAAATCGTGC
    TATTTCCTTG TGTCTGCTTG GCAGATCCGA AATGGTTATC CTGAACTTTG TATTGGAAAT
    CTATTAACAC ATTCATACGG AATGACCAAT ATGATTGCAT TCAAAGTATT CATGAATATT
    CCATTCCTCT TCGAGCTTCG TACAGCTATT GATTGGACAT GGACAGATAC TTCAATGCCT
    CTTTTCGATT TCTTCAATAT GGAAAATTTC TATGCTCATA TATTCAATAT CAAGTGTGCC
    CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA AGAGGGATTC CCAAAGGAAA GTTGGTCAAG
    TATATGATGG GATTCCCAAT TATCATTGGA GTCGTTATCT TCATCTTTTC TCCTCTATTG
    CTCTGGTCGC TACTCAATCA AATCGGAACT ATCAGTATGC CAGAAAAAGT TACGTTGAGG
    ATTTCGATTG AAGGATATCC ACCACTTTAC GAGATGGAGG CACAAGGAAG TAATCACGAT
    AACGCTGAAC TTGGAATGAT AAAACCTGAT CAATTGGCAT CTTTGAATCA AGCATTGACG
    GATAGTTACA CGACACGAGA CACTAATTCT ATTTTAAGAT CAAGAATGTC TGTATCATAT
    CTGAAAGGAT ACACATATGA GGATATTCTG ATTGTGCGAT TCCGTCCAGA ATCTGAAATT
    TATTGGCCGA TTTCACAGGA TTCGAGAAAT GCGATGATCG ATAAATTGAG CCGAAACACG
    TCAGTCAATT TTGAAGTATC TCTGGAATTC ACTCGGCCAT ATGATCCAAA TGAAAACGCT
    GCTTTAAAAC ATTCGAAATC GTGGCTTGTT CCAATCTCCC TGGATATGAC GATTCGAGCA
    AAAATTCAAA GTGCTCTCCG AGGAGATCCA GGTCATCCGA TTCTCATTCC ACAGTCAATT
    CCTGCGTTTA TTCAAGTTCC AAATCAAGGA GAATTGACCC TTCCAACATC AATCGGAAAT
    ACTATTATCA ATGACGGAAA TCCACGGATT AATACGACCG GGATGGAAAA ATCAGACGAA
    GCTCGGGCTT GGTTCGACTC GTTAACCTTG AATCTCGAAC AAGGAAAATC GCAAAACGAG
    AAAATGTGGA TCGCCACATC CGAACATCCA GGAGACCAAA ATGCGAAACT CTGGATAAAG
    ACTGCAAATA CGACGTATTC GGGAAGGCCG TACCTTCAAG TTGTCGGATT TATTGATAGA
    GCATTCCCAT CATTTTTGGC GAAAGTTTTC AAAGGAGGAG TCATTGCAGT GTATCTTTCA
    GTTATCCTCG TCGTTGGAAG AGGTCTGGTT CGTGGAATCT TCACCACATC TCCAAGCACT
    GTCATGTTCA CAGAATTACC GAATGCGGAT CATCTTCTCA AAATTTGTCT CGACATCTAT
    TTGGTTCGAG AAGCGAAGGA CTTCATGTTG GAGCAGGATC TCTTCGCCAA ATTAATATTC
    CTGTTCCGAT CACCAGCGAC ACTCATCGAA TGGACACGAA TGAGCAAAAA GAAGCAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255387.2
    CDS57..7379
    Translation

    Target ORF information:

    RefSeq Version NM_001268458.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268458.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACTACAGCAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGGATCCAAA AAGTCGGAAG
    TTCGCGGCAT TTGTGGAATA TCTGTCGAAT AAAGTGTCTG TCTACTTCAT ATTTGTAGTA
    TCAGTGGTAC TTTTGGTGGT CTCTACGTGT TTTGCTCCGA ATTTCTACAA TATTCTATTC
    TTCGCACTAT GGGCATTGAA CTTGATTTAT CTCAAGTTCT CATTCCGTCT GTATCGTGGA
    TTGGCATATG CATTCTGGCT AACTCTCACG TTCTACACTT CAATTGTTAT TATCGCCCTC
    TACATTTATC AGTTCCCTGG AGTATCTCAA TGGATTATTC GAAATACTAG TTTGTCGCAG
    GAGTGGCTGA ATGCAATTGG ACTTGTCGAT TTTAGAGCTA TCGGTGAATC GGGAGCCCTA
    TTTCTACAAT TACTCGCCCC AATTGCCTTG TTCGTCGTCA CAATGCTCCA ACTCAAGTTT
    TTCCATGGAC CATGGAGTCG AGCAACGTCA CCAAGAAGAG CAGAAAATGA TCCACCTACA
    AGTACGACGG AGGCTGCGGC GGTAGCAAGC ACATCTGGAA CACAAGGAAG AGCACATGCT
    GCAGGCGATA CCCTTGTCAA GAAGCTGCAC AAATTGGCAA ATCAGACAAT CGAACTGTTA
    TGGAGGTTTT TCGAAGTTCA CATTTCGAAA ATTGTCTTTG TCATCATTGC GATTTTTATT
    GCCAACAATA TCAATGCTCT ATACATTCCA CTTGTTATTC TCCTCTCATT GGCTATATGT
    CTACCATCAG CTGCAGATGG GATATTCTCT TTATTTATGT GTGCCTACTT ATTCCTCGTG
    GCACTGAGCA AGATGATCTA CCAACTGGAT ATTGTTCCCG AGTTATCACA AATCGACCGA
    GGAGTTGGAG CAGATAACTG TTCACATGGA AATATTTCAA TGCCTGAATG GTTTGGATTG
    AAGAAGGAAG TTGAAGGAAC TGAACCAATT TATATGCTCT TTGGAGTCAT TGTTTCGATT
    ATTGCATTGG CTTTTCAATC AATTGTTATA TATCGACAAA GACATTATCG AGCTTCACTG
    GGACTTCCTG AATCGATGCG AGCTAAAGTG TTCCCGGACT TCCATCATTC TCATTTTGAT
    AGATCTCTCA AAAATGCCAT CCAATTCTTG ATAGACTATG GTTTCTATAA GTTTGGACTT
    GAAATCACGA TGATCGCCAT TGGAATTGAC ATTTTCAATC GAATGGATGC ATTGGCAGCG
    ATTCAATGTT TCTGGCTTGT CCTGTTTGCG CTCAATAAAA GAGTCTTTGT TCGAAGAATA
    TGGGTATTCT ATGTGATCTA TATGGCGATT CTGTATCCAC TTCAGTTCTT TTCATATGTT
    GGATTACCAC CTGACAGTTG TATTGAGTAT CCATGGTCGT ATTGGATTCC ATCGTATAGT
    GATGATGCGA GATTCAATCT GTCGTATCTT CTGAACTTGT CAATATACGG TGTAAACTGG
    CCATCAGCAT ATCTCATTGG CGATTTCTTC GTACTCCTCT TGGCATCATG CCAATTAGCA
    GTTTTCCGGC GAGAAGGAGA AGATAATGAT TCAATTTACA ATGATGGAAA CTTTGTGATT
    AAGCCAGAAA ATCCACAATA TGACTTTATT GACACCAAAA AGAGTTACGT TGATTATTTC
    AAATCATTCG TTTTCCATTA TGGACACTGG ATTACATTGA TGTCTACACT AGCAGCAGGA
    ATTGCTGGAA CGTCACTTTT TGCTTTGGGA TACATTATAT TCACTCTGAC AATGCTGTGG
    TCTGGTAACA ATTTGTATGT GATGAACTCA ACTCTACGAT CATTTGAACA TACTTTGAAA
    CGATGGAATG CACTTTTGGG ATACACATTG TTCACGATTA CAATGAAAGT TTGCCTTCAA
    ATATTTGGAT GTGTATTCCT GTCGTGGTTC GATCAATCTG GTGGATGGGG AAAGACTCTG
    TGCATCGTTC GTCAACTCTT CAGTATCACA TGTGTTAATA ACGAATGTCA TGTGCTCAAA
    GAGCTGGAGG ATTTCTCGAA GGCGTGTGCT GTTGAGACGA AAGAAGGAAA TATTGGATTT
    GATGTGATTG CGCTATCCTT CCTCGTCTTC CAAATCCGAA TTTTCCATTC TTGGTATTTC
    CAACACTGCA TGGTTGAATA TCGAAGTGAG GTTATTCTGG CCAATAGAGG TGCCGTGCTG
    AAGAATCAAC TGATTGAAAA GGAAATGAAG GAACAAAACG AGCAGCAAAA AGCCAAGTTC
    AATGATATCA GAAGAAGAAC TGAAGCTATT CGTGAAAGAT ATCAAAAACA AATCGAACGA
    GGAGCTGCTG AACGTGACTT CGAGCCGGTT ACTTATGGGC ACGCCAAACG AGCGGGAGAC
    TACTACATGT TCAAATACGA TCCTGAAAAT GATGATCTTG TGGAACCCGT CGACAGTTTT
    GTACCAGAAG TTGATCCCAA GGCAACTGCA TATGATCGAC TTGATCCTGG ACAGATAATG
    TACGCTGCCA CCGCTCACGA CCTCGACCTC GCCAAAACTG TACAGCAAGT CAAGAAAGGC
    GATACTATCA AAGATCCTGA TTCACGTGCT CTTATCGCTG TTTCTGAACC AGAAGCTCGT
    AAGCCAGGAG GCACTGAAGA AACGGATGGT GATGAAGATG AGGATAATAA GGATTCGAAA
    GTGGAAAGTA CTGCTAAATT CATTCAAAAA ATGATCGCTT CTGCCTTGGA CTTGTGTAGT
    GTAACACTAA ACAAACTGTG CAGAGAACAC CGATATGTTG GATTTGTTTT GAGCAAGGAA
    AAACAAAAGT TGAAGTCAGG ACACTCGGAA TCTCTTTCAA ATACATCTAG AAAGCTGACG
    GATATTCGAT CTGCAGTTGA TCTACCAAGT CTTCAACTTG TGCAATCGGC GAATGATGTT
    GAAAAGATGG AGACAGCCGT CAGCGTGGAT TGGCAACAAA AATCATCAGC AACGAGACTA
    TTGAATGCAG TTGTGAATTG TATTGGAGCA CACACTGATA TTCTCTGCTA CTTCTTCGCA
    ATTATGACAC AAGTGATGAC TGGTGGACTT ATTACTCTTC CACTTCCTCT GATGTCACTA
    TTCTGGGGAA ATCTATCGAA TCCTCGTCCA TCCAAGTTCT TCTGGGTCAC AATGATCACC
    TACACTGAGT GTGTCATTGT TATCAAGTTT GTTTGCCAAT TTGCATTCAT GCCTTACAAT
    TCTATCACGT GGCGAACAGA GCATCAAATG GACCCGATGT CATTGGATAA GTTATTCGGA
    GTATCACAGA GAGATAGTTT TGCACTTTGG GACATTGTTT TGCTCTTCTC ACTCTTCTTC
    CATCGGTATA TGCTCAGAAA GTTAGGGTTA TGGAAGGATG CCAATTTGAC GGACACGTTC
    ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA GGATCGGACA CAGGATCACC CAAGAAAATT
    GCTCAAGAGC CAAAGGTTGT TGTAACTCAG TCAGATACCC TTGAAGGTAC AAGTGGAGGA
    GAGATAGTCA TACCATCTGA TCCAAATGCA GTTTCCAATA TGGAAGAATT AGACTGCGAA
    CCTCCGATTC CAGAGAAGCA AAGTGGACCA ATTGGAAGAT TCATTCATCA ATTATTCCAT
    CCAAAATTCC GTTACATTCG TGATTTGTAT CCAATAATGT TTGGAATCGA TGTGATATGC
    TTCCTCATCA TGACATTTGG ATACTCGGCA TTTGGTGAAG GAGGTTCTGG AAATGTTTTA
    GATGATGTGA AGGCTTCTCG TATTCCGGTA ACACTTGTTG TTATGCTCGT TGGAATGACT
    CTGGCAATTA TCATTGATCG AGCACTCTAC CTCAGAAAAT CAGTTGTCGG AAAGCTAATC
    TATCAAGTCT TAATGATTGC TTTCCTTCAC ATTTGGGTAT TCCTTGTTCT TCCAAATATG
    ACTCGTCGTT CTGCGATATC GAATCACGTT GCTCAAGCAT TGTACGTCAT CAAATCGTGC
    TATTTCCTTG TGTCTGCTTG GCAGATCCGA AATGGTTATC CTGAACTTTG TATTGGAAAT
    CTATTAACAC ATTCATACGG AATGACCAAT ATGATTGCAT TCAAAGTATT CATGAATATT
    CCATTCCTCT TCGAGCTTCG TACAGCTATT GATTGGACAT GGACAGATAC TTCAATGCCT
    CTTTTCGATT TCTTCAATAT GGAAAATTTC TATGCTCATA TATTCAATAT CAAGTGTGCC
    CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA AGAGGGATTC CCAAAGGAAA GTTGGTCAAG
    TATATGATGG GATTCCCAAT TATCATTGGA GTCGTTATCT TCATCTTTTC TCCTCTATTG
    CTCTGGTCGC TACTCAATCA AATCGGAACT ATCAGTATGC CAGAAAAAGT TACGTTGAGG
    ATTTCGATTG AAGGATATCC ACCACTTTAC GAGATGGAGG CACAAGGAAG TAATCACGAT
    AACGCTGAAC TTGGAATGAT AAAACCTGAT CAATTGGCAT CTTTGAATCA AGCATTGACG
    GATAGTTACA CGACACGAGA CACTAATTCT ATTTTAAGAT CAAGAATGTC TGTATCATAT
    CTGAAAGGAT ACACATATGA GGATATTCTG ATTGTGCGAT TCCGTCCAGA ATCTGAAATT
    TATTGGCCGA TTTCACAGGA TTCGAGAAAT GCGATGATCG ATAAATTGAG CCGAAACACG
    TCAGTCAATT TTGAAGTATC TCTGGAATTC ACTCGGCCAT ATGATCCAAA TGAAAACGCT
    GCTTTAAAAC ATTCGAAATC GTGGCTTGTT CCAATCTCCC TGGATATGAC GATTCGAGCA
    AAAATTCAAA GTGCTCTCCG AGGAGATCCA GGTCATCCGA TTCTCATTCC ACAGTCAATT
    CCTGCGTTTA TTCAAGTTCC AAATCAAGGA GAATTGACCC TTCCAACATC AATCGGAAAT
    ACTATTATCA ATGACGGAAA TCCACGGATT AATACGACCG GGATGGAAAA ATCAGACGAA
    GCTCGGGCTT GGTTCGACTC GTTAACCTTG AATCTCGAAC AAGGAAAATC GCAAAACGAG
    AAAATGTGGA TCGCCACATC CGAACATCCA GGAGACCAAA ATGCGAAACT CTGGATAAAG
    ACTGCAAATA CGACGTATTC GGGAAGGCCG TACCTTCAAG TTGTCGGATT TATTGATAGA
    GCATTCCCAT CATTTTTGGC GAAAGTTTTC AAAGGAGGAG TCATTGCAGT GTATCTTTCA
    GTTATCCTCG TCGTTGGAAG AGGTCTGGTT CGTGGAATCT TCACCACATC TCCAAGCACT
    GTCATGTTCA CAGAATTACC GAATGCGGAT CATCTTCTCA AAATTTGTCT CGACATCTAT
    TTGGTTCGAG AAGCGAAGGA CTTCATGTTG GAGCAGGATC TCTTCGCCAA ATTAATATTC
    CTGTTCCGAT CACCAGCGAC ACTCATCGAA TGGACACGAA TGAGCAAAAA GAAGCAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342182
    Clone ID Related Accession (Same CDS sequence) NM_001268458.3 , NM_001268458.2
    Accession Version NM_001268458.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Apr 20, 2015 this sequence version replaced NM_001268458.1. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACTACAGCAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGGATCCAAA AAGTCGGAAG
    TTCGCGGCAT TTGTGGAATA TCTGTCGAAT AAAGTGTCTG TCTACTTCAT ATTTGTAGTA
    TCAGTGGTAC TTTTGGTGGT CTCTACGTGT TTTGCTCCGA ATTTCTACAA TATTCTATTC
    TTCGCACTAT GGGCATTGAA CTTGATTTAT CTCAAGTTCT CATTCCGTCT GTATCGTGGA
    TTGGCATATG CATTCTGGCT AACTCTCACG TTCTACACTT CAATTGTTAT TATCGCCCTC
    TACATTTATC AGTTCCCTGG AGTATCTCAA TGGATTATTC GAAATACTAG TTTGTCGCAG
    GAGTGGCTGA ATGCAATTGG ACTTGTCGAT TTTAGAGCTA TCGGTGAATC GGGAGCCCTA
    TTTCTACAAT TACTCGCCCC AATTGCCTTG TTCGTCGTCA CAATGCTCCA ACTCAAGTTT
    TTCCATGGAC CATGGAGTCG AGCAACGTCA CCAAGAAGAG CAGAAAATGA TCCACCTACA
    AGTACGACGG AGGCTGCGGC GGTAGCAAGC ACATCTGGAA CACAAGGAAG AGCACATGCT
    GCAGGCGATA CCCTTGTCAA GAAGCTGCAC AAATTGGCAA ATCAGACAAT CGAACTGTTA
    TGGAGGTTTT TCGAAGTTCA CATTTCGAAA ATTGTCTTTG TCATCATTGC GATTTTTATT
    GCCAACAATA TCAATGCTCT ATACATTCCA CTTGTTATTC TCCTCTCATT GGCTATATGT
    CTACCATCAG CTGCAGATGG GATATTCTCT TTATTTATGT GTGCCTACTT ATTCCTCGTG
    GCACTGAGCA AGATGATCTA CCAACTGGAT ATTGTTCCCG AGTTATCACA AATCGACCGA
    GGAGTTGGAG CAGATAACTG TTCACATGGA AATATTTCAA TGCCTGAATG GTTTGGATTG
    AAGAAGGAAG TTGAAGGAAC TGAACCAATT TATATGCTCT TTGGAGTCAT TGTTTCGATT
    ATTGCATTGG CTTTTCAATC AATTGTTATA TATCGACAAA GACATTATCG AGCTTCACTG
    GGACTTCCTG AATCGATGCG AGCTAAAGTG TTCCCGGACT TCCATCATTC TCATTTTGAT
    AGATCTCTCA AAAATGCCAT CCAATTCTTG ATAGACTATG GTTTCTATAA GTTTGGACTT
    GAAATCACGA TGATCGCCAT TGGAATTGAC ATTTTCAATC GAATGGATGC ATTGGCAGCG
    ATTCAATGTT TCTGGCTTGT CCTGTTTGCG CTCAATAAAA GAGTCTTTGT TCGAAGAATA
    TGGGTATTCT ATGTGATCTA TATGGCGATT CTGTATCCAC TTCAGTTCTT TTCATATGTT
    GGATTACCAC CTGACAGTTG TATTGAGTAT CCATGGTCGT ATTGGATTCC ATCGTATAGT
    GATGATGCGA GATTCAATCT GTCGTATCTT CTGAACTTGT CAATATACGG TGTAAACTGG
    CCATCAGCAT ATCTCATTGG CGATTTCTTC GTACTCCTCT TGGCATCATG CCAATTAGCA
    GTTTTCCGGC GAGAAGGAGA AGATAATGAT TCAATTTACA ATGATGGAAA CTTTGTGATT
    AAGCCAGAAA ATCCACAATA TGACTTTATT GACACCAAAA AGAGTTACGT TGATTATTTC
    AAATCATTCG TTTTCCATTA TGGACACTGG ATTACATTGA TGTCTACACT AGCAGCAGGA
    ATTGCTGGAA CGTCACTTTT TGCTTTGGGA TACATTATAT TCACTCTGAC AATGCTGTGG
    TCTGGTAACA ATTTGTATGT GATGAACTCA ACTCTACGAT CATTTGAACA TACTTTGAAA
    CGATGGAATG CACTTTTGGG ATACACATTG TTCACGATTA CAATGAAAGT TTGCCTTCAA
    ATATTTGGAT GTGTATTCCT GTCGTGGTTC GATCAATCTG GTGGATGGGG AAAGACTCTG
    TGCATCGTTC GTCAACTCTT CAGTATCACA TGTGTTAATA ACGAATGTCA TGTGCTCAAA
    GAGCTGGAGG ATTTCTCGAA GGCGTGTGCT GTTGAGACGA AAGAAGGAAA TATTGGATTT
    GATGTGATTG CGCTATCCTT CCTCGTCTTC CAAATCCGAA TTTTCCATTC TTGGTATTTC
    CAACACTGCA TGGTTGAATA TCGAAGTGAG GTTATTCTGG CCAATAGAGG TGCCGTGCTG
    AAGAATCAAC TGATTGAAAA GGAAATGAAG GAACAAAACG AGCAGCAAAA AGCCAAGTTC
    AATGATATCA GAAGAAGAAC TGAAGCTATT CGTGAAAGAT ATCAAAAACA AATCGAACGA
    GGAGCTGCTG AACGTGACTT CGAGCCGGTT ACTTATGGGC ACGCCAAACG AGCGGGAGAC
    TACTACATGT TCAAATACGA TCCTGAAAAT GATGATCTTG TGGAACCCGT CGACAGTTTT
    GTACCAGAAG TTGATCCCAA GGCAACTGCA TATGATCGAC TTGATCCTGG ACAGATAATG
    TACGCTGCCA CCGCTCACGA CCTCGACCTC GCCAAAACTG TACAGCAAGT CAAGAAAGGC
    GATACTATCA AAGATCCTGA TTCACGTGCT CTTATCGCTG TTTCTGAACC AGAAGCTCGT
    AAGCCAGGAG GCACTGAAGA AACGGATGGT GATGAAGATG AGGATAATAA GGATTCGAAA
    GTGGAAAGTA CTGCTAAATT CATTCAAAAA ATGATCGCTT CTGCCTTGGA CTTGTGTAGT
    GTAACACTAA ACAAACTGTG CAGAGAACAC CGATATGTTG GATTTGTTTT GAGCAAGGAA
    AAACAAAAGT TGAAGTCAGG ACACTCGGAA TCTCTTTCAA ATACATCTAG AAAGCTGACG
    GATATTCGAT CTGCAGTTGA TCTACCAAGT CTTCAACTTG TGCAATCGGC GAATGATGTT
    GAAAAGATGG AGACAGCCGT CAGCGTGGAT TGGCAACAAA AATCATCAGC AACGAGACTA
    TTGAATGCAG TTGTGAATTG TATTGGAGCA CACACTGATA TTCTCTGCTA CTTCTTCGCA
    ATTATGACAC AAGTGATGAC TGGTGGACTT ATTACTCTTC CACTTCCTCT GATGTCACTA
    TTCTGGGGAA ATCTATCGAA TCCTCGTCCA TCCAAGTTCT TCTGGGTCAC AATGATCACC
    TACACTGAGT GTGTCATTGT TATCAAGTTT GTTTGCCAAT TTGCATTCAT GCCTTACAAT
    TCTATCACGT GGCGAACAGA GCATCAAATG GACCCGATGT CATTGGATAA GTTATTCGGA
    GTATCACAGA GAGATAGTTT TGCACTTTGG GACATTGTTT TGCTCTTCTC ACTCTTCTTC
    CATCGGTATA TGCTCAGAAA GTTAGGGTTA TGGAAGGATG CCAATTTGAC GGACACGTTC
    ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA GGATCGGACA CAGGATCACC CAAGAAAATT
    GCTCAAGAGC CAAAGGTTGT TGTAACTCAG TCAGATACCC TTGAAGGTAC AAGTGGAGGA
    GAGATAGTCA TACCATCTGA TCCAAATGCA GTTTCCAATA TGGAAGAATT AGACTGCGAA
    CCTCCGATTC CAGAGAAGCA AAGTGGACCA ATTGGAAGAT TCATTCATCA ATTATTCCAT
    CCAAAATTCC GTTACATTCG TGATTTGTAT CCAATAATGT TTGGAATCGA TGTGATATGC
    TTCCTCATCA TGACATTTGG ATACTCGGCA TTTGGTGAAG GAGGTTCTGG AAATGTTTTA
    GATGATGTGA AGGCTTCTCG TATTCCGGTA ACACTTGTTG TTATGCTCGT TGGAATGACT
    CTGGCAATTA TCATTGATCG AGCACTCTAC CTCAGAAAAT CAGTTGTCGG AAAGCTAATC
    TATCAAGTCT TAATGATTGC TTTCCTTCAC ATTTGGGTAT TCCTTGTTCT TCCAAATATG
    ACTCGTCGTT CTGCGATATC GAATCACGTT GCTCAAGCAT TGTACGTCAT CAAATCGTGC
    TATTTCCTTG TGTCTGCTTG GCAGATCCGA AATGGTTATC CTGAACTTTG TATTGGAAAT
    CTATTAACAC ATTCATACGG AATGACCAAT ATGATTGCAT TCAAAGTATT CATGAATATT
    CCATTCCTCT TCGAGCTTCG TACAGCTATT GATTGGACAT GGACAGATAC TTCAATGCCT
    CTTTTCGATT TCTTCAATAT GGAAAATTTC TATGCTCATA TATTCAATAT CAAGTGTGCC
    CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA AGAGGGATTC CCAAAGGAAA GTTGGTCAAG
    TATATGATGG GATTCCCAAT TATCATTGGA GTCGTTATCT TCATCTTTTC TCCTCTATTG
    CTCTGGTCGC TACTCAATCA AATCGGAACT ATCAGTATGC CAGAAAAAGT TACGTTGAGG
    ATTTCGATTG AAGGATATCC ACCACTTTAC GAGATGGAGG CACAAGGAAG TAATCACGAT
    AACGCTGAAC TTGGAATGAT AAAACCTGAT CAATTGGCAT CTTTGAATCA AGCATTGACG
    GATAGTTACA CGACACGAGA CACTAATTCT ATTTTAAGAT CAAGAATGTC TGTATCATAT
    CTGAAAGGAT ACACATATGA GGATATTCTG ATTGTGCGAT TCCGTCCAGA ATCTGAAATT
    TATTGGCCGA TTTCACAGGA TTCGAGAAAT GCGATGATCG ATAAATTGAG CCGAAACACG
    TCAGTCAATT TTGAAGTATC TCTGGAATTC ACTCGGCCAT ATGATCCAAA TGAAAACGCT
    GCTTTAAAAC ATTCGAAATC GTGGCTTGTT CCAATCTCCC TGGATATGAC GATTCGAGCA
    AAAATTCAAA GTGCTCTCCG AGGAGATCCA GGTCATCCGA TTCTCATTCC ACAGTCAATT
    CCTGCGTTTA TTCAAGTTCC AAATCAAGGA GAATTGACCC TTCCAACATC AATCGGAAAT
    ACTATTATCA ATGACGGAAA TCCACGGATT AATACGACCG GGATGGAAAA ATCAGACGAA
    GCTCGGGCTT GGTTCGACTC GTTAACCTTG AATCTCGAAC AAGGAAAATC GCAAAACGAG
    AAAATGTGGA TCGCCACATC CGAACATCCA GGAGACCAAA ATGCGAAACT CTGGATAAAG
    ACTGCAAATA CGACGTATTC GGGAAGGCCG TACCTTCAAG TTGTCGGATT TATTGATAGA
    GCATTCCCAT CATTTTTGGC GAAAGTTTTC AAAGGAGGAG TCATTGCAGT GTATCTTTCA
    GTTATCCTCG TCGTTGGAAG AGGTCTGGTT CGTGGAATCT TCACCACATC TCCAAGCACT
    GTCATGTTCA CAGAATTACC GAATGCGGAT CATCTTCTCA AAATTTGTCT CGACATCTAT
    TTGGTTCGAG AAGCGAAGGA CTTCATGTTG GAGCAGGATC TCTTCGCCAA ATTAATATTC
    CTGTTCCGAT CACCAGCGAC ACTCATCGAA TGGACACGAA TGAGCAAAAA GAAGCAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255387.2
    CDS1..7323
    Translation

    Target ORF information:

    RefSeq Version NM_001268458.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268458.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACTACAGCAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGGATCCAAA AAGTCGGAAG
    TTCGCGGCAT TTGTGGAATA TCTGTCGAAT AAAGTGTCTG TCTACTTCAT ATTTGTAGTA
    TCAGTGGTAC TTTTGGTGGT CTCTACGTGT TTTGCTCCGA ATTTCTACAA TATTCTATTC
    TTCGCACTAT GGGCATTGAA CTTGATTTAT CTCAAGTTCT CATTCCGTCT GTATCGTGGA
    TTGGCATATG CATTCTGGCT AACTCTCACG TTCTACACTT CAATTGTTAT TATCGCCCTC
    TACATTTATC AGTTCCCTGG AGTATCTCAA TGGATTATTC GAAATACTAG TTTGTCGCAG
    GAGTGGCTGA ATGCAATTGG ACTTGTCGAT TTTAGAGCTA TCGGTGAATC GGGAGCCCTA
    TTTCTACAAT TACTCGCCCC AATTGCCTTG TTCGTCGTCA CAATGCTCCA ACTCAAGTTT
    TTCCATGGAC CATGGAGTCG AGCAACGTCA CCAAGAAGAG CAGAAAATGA TCCACCTACA
    AGTACGACGG AGGCTGCGGC GGTAGCAAGC ACATCTGGAA CACAAGGAAG AGCACATGCT
    GCAGGCGATA CCCTTGTCAA GAAGCTGCAC AAATTGGCAA ATCAGACAAT CGAACTGTTA
    TGGAGGTTTT TCGAAGTTCA CATTTCGAAA ATTGTCTTTG TCATCATTGC GATTTTTATT
    GCCAACAATA TCAATGCTCT ATACATTCCA CTTGTTATTC TCCTCTCATT GGCTATATGT
    CTACCATCAG CTGCAGATGG GATATTCTCT TTATTTATGT GTGCCTACTT ATTCCTCGTG
    GCACTGAGCA AGATGATCTA CCAACTGGAT ATTGTTCCCG AGTTATCACA AATCGACCGA
    GGAGTTGGAG CAGATAACTG TTCACATGGA AATATTTCAA TGCCTGAATG GTTTGGATTG
    AAGAAGGAAG TTGAAGGAAC TGAACCAATT TATATGCTCT TTGGAGTCAT TGTTTCGATT
    ATTGCATTGG CTTTTCAATC AATTGTTATA TATCGACAAA GACATTATCG AGCTTCACTG
    GGACTTCCTG AATCGATGCG AGCTAAAGTG TTCCCGGACT TCCATCATTC TCATTTTGAT
    AGATCTCTCA AAAATGCCAT CCAATTCTTG ATAGACTATG GTTTCTATAA GTTTGGACTT
    GAAATCACGA TGATCGCCAT TGGAATTGAC ATTTTCAATC GAATGGATGC ATTGGCAGCG
    ATTCAATGTT TCTGGCTTGT CCTGTTTGCG CTCAATAAAA GAGTCTTTGT TCGAAGAATA
    TGGGTATTCT ATGTGATCTA TATGGCGATT CTGTATCCAC TTCAGTTCTT TTCATATGTT
    GGATTACCAC CTGACAGTTG TATTGAGTAT CCATGGTCGT ATTGGATTCC ATCGTATAGT
    GATGATGCGA GATTCAATCT GTCGTATCTT CTGAACTTGT CAATATACGG TGTAAACTGG
    CCATCAGCAT ATCTCATTGG CGATTTCTTC GTACTCCTCT TGGCATCATG CCAATTAGCA
    GTTTTCCGGC GAGAAGGAGA AGATAATGAT TCAATTTACA ATGATGGAAA CTTTGTGATT
    AAGCCAGAAA ATCCACAATA TGACTTTATT GACACCAAAA AGAGTTACGT TGATTATTTC
    AAATCATTCG TTTTCCATTA TGGACACTGG ATTACATTGA TGTCTACACT AGCAGCAGGA
    ATTGCTGGAA CGTCACTTTT TGCTTTGGGA TACATTATAT TCACTCTGAC AATGCTGTGG
    TCTGGTAACA ATTTGTATGT GATGAACTCA ACTCTACGAT CATTTGAACA TACTTTGAAA
    CGATGGAATG CACTTTTGGG ATACACATTG TTCACGATTA CAATGAAAGT TTGCCTTCAA
    ATATTTGGAT GTGTATTCCT GTCGTGGTTC GATCAATCTG GTGGATGGGG AAAGACTCTG
    TGCATCGTTC GTCAACTCTT CAGTATCACA TGTGTTAATA ACGAATGTCA TGTGCTCAAA
    GAGCTGGAGG ATTTCTCGAA GGCGTGTGCT GTTGAGACGA AAGAAGGAAA TATTGGATTT
    GATGTGATTG CGCTATCCTT CCTCGTCTTC CAAATCCGAA TTTTCCATTC TTGGTATTTC
    CAACACTGCA TGGTTGAATA TCGAAGTGAG GTTATTCTGG CCAATAGAGG TGCCGTGCTG
    AAGAATCAAC TGATTGAAAA GGAAATGAAG GAACAAAACG AGCAGCAAAA AGCCAAGTTC
    AATGATATCA GAAGAAGAAC TGAAGCTATT CGTGAAAGAT ATCAAAAACA AATCGAACGA
    GGAGCTGCTG AACGTGACTT CGAGCCGGTT ACTTATGGGC ACGCCAAACG AGCGGGAGAC
    TACTACATGT TCAAATACGA TCCTGAAAAT GATGATCTTG TGGAACCCGT CGACAGTTTT
    GTACCAGAAG TTGATCCCAA GGCAACTGCA TATGATCGAC TTGATCCTGG ACAGATAATG
    TACGCTGCCA CCGCTCACGA CCTCGACCTC GCCAAAACTG TACAGCAAGT CAAGAAAGGC
    GATACTATCA AAGATCCTGA TTCACGTGCT CTTATCGCTG TTTCTGAACC AGAAGCTCGT
    AAGCCAGGAG GCACTGAAGA AACGGATGGT GATGAAGATG AGGATAATAA GGATTCGAAA
    GTGGAAAGTA CTGCTAAATT CATTCAAAAA ATGATCGCTT CTGCCTTGGA CTTGTGTAGT
    GTAACACTAA ACAAACTGTG CAGAGAACAC CGATATGTTG GATTTGTTTT GAGCAAGGAA
    AAACAAAAGT TGAAGTCAGG ACACTCGGAA TCTCTTTCAA ATACATCTAG AAAGCTGACG
    GATATTCGAT CTGCAGTTGA TCTACCAAGT CTTCAACTTG TGCAATCGGC GAATGATGTT
    GAAAAGATGG AGACAGCCGT CAGCGTGGAT TGGCAACAAA AATCATCAGC AACGAGACTA
    TTGAATGCAG TTGTGAATTG TATTGGAGCA CACACTGATA TTCTCTGCTA CTTCTTCGCA
    ATTATGACAC AAGTGATGAC TGGTGGACTT ATTACTCTTC CACTTCCTCT GATGTCACTA
    TTCTGGGGAA ATCTATCGAA TCCTCGTCCA TCCAAGTTCT TCTGGGTCAC AATGATCACC
    TACACTGAGT GTGTCATTGT TATCAAGTTT GTTTGCCAAT TTGCATTCAT GCCTTACAAT
    TCTATCACGT GGCGAACAGA GCATCAAATG GACCCGATGT CATTGGATAA GTTATTCGGA
    GTATCACAGA GAGATAGTTT TGCACTTTGG GACATTGTTT TGCTCTTCTC ACTCTTCTTC
    CATCGGTATA TGCTCAGAAA GTTAGGGTTA TGGAAGGATG CCAATTTGAC GGACACGTTC
    ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA GGATCGGACA CAGGATCACC CAAGAAAATT
    GCTCAAGAGC CAAAGGTTGT TGTAACTCAG TCAGATACCC TTGAAGGTAC AAGTGGAGGA
    GAGATAGTCA TACCATCTGA TCCAAATGCA GTTTCCAATA TGGAAGAATT AGACTGCGAA
    CCTCCGATTC CAGAGAAGCA AAGTGGACCA ATTGGAAGAT TCATTCATCA ATTATTCCAT
    CCAAAATTCC GTTACATTCG TGATTTGTAT CCAATAATGT TTGGAATCGA TGTGATATGC
    TTCCTCATCA TGACATTTGG ATACTCGGCA TTTGGTGAAG GAGGTTCTGG AAATGTTTTA
    GATGATGTGA AGGCTTCTCG TATTCCGGTA ACACTTGTTG TTATGCTCGT TGGAATGACT
    CTGGCAATTA TCATTGATCG AGCACTCTAC CTCAGAAAAT CAGTTGTCGG AAAGCTAATC
    TATCAAGTCT TAATGATTGC TTTCCTTCAC ATTTGGGTAT TCCTTGTTCT TCCAAATATG
    ACTCGTCGTT CTGCGATATC GAATCACGTT GCTCAAGCAT TGTACGTCAT CAAATCGTGC
    TATTTCCTTG TGTCTGCTTG GCAGATCCGA AATGGTTATC CTGAACTTTG TATTGGAAAT
    CTATTAACAC ATTCATACGG AATGACCAAT ATGATTGCAT TCAAAGTATT CATGAATATT
    CCATTCCTCT TCGAGCTTCG TACAGCTATT GATTGGACAT GGACAGATAC TTCAATGCCT
    CTTTTCGATT TCTTCAATAT GGAAAATTTC TATGCTCATA TATTCAATAT CAAGTGTGCC
    CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA AGAGGGATTC CCAAAGGAAA GTTGGTCAAG
    TATATGATGG GATTCCCAAT TATCATTGGA GTCGTTATCT TCATCTTTTC TCCTCTATTG
    CTCTGGTCGC TACTCAATCA AATCGGAACT ATCAGTATGC CAGAAAAAGT TACGTTGAGG
    ATTTCGATTG AAGGATATCC ACCACTTTAC GAGATGGAGG CACAAGGAAG TAATCACGAT
    AACGCTGAAC TTGGAATGAT AAAACCTGAT CAATTGGCAT CTTTGAATCA AGCATTGACG
    GATAGTTACA CGACACGAGA CACTAATTCT ATTTTAAGAT CAAGAATGTC TGTATCATAT
    CTGAAAGGAT ACACATATGA GGATATTCTG ATTGTGCGAT TCCGTCCAGA ATCTGAAATT
    TATTGGCCGA TTTCACAGGA TTCGAGAAAT GCGATGATCG ATAAATTGAG CCGAAACACG
    TCAGTCAATT TTGAAGTATC TCTGGAATTC ACTCGGCCAT ATGATCCAAA TGAAAACGCT
    GCTTTAAAAC ATTCGAAATC GTGGCTTGTT CCAATCTCCC TGGATATGAC GATTCGAGCA
    AAAATTCAAA GTGCTCTCCG AGGAGATCCA GGTCATCCGA TTCTCATTCC ACAGTCAATT
    CCTGCGTTTA TTCAAGTTCC AAATCAAGGA GAATTGACCC TTCCAACATC AATCGGAAAT
    ACTATTATCA ATGACGGAAA TCCACGGATT AATACGACCG GGATGGAAAA ATCAGACGAA
    GCTCGGGCTT GGTTCGACTC GTTAACCTTG AATCTCGAAC AAGGAAAATC GCAAAACGAG
    AAAATGTGGA TCGCCACATC CGAACATCCA GGAGACCAAA ATGCGAAACT CTGGATAAAG
    ACTGCAAATA CGACGTATTC GGGAAGGCCG TACCTTCAAG TTGTCGGATT TATTGATAGA
    GCATTCCCAT CATTTTTGGC GAAAGTTTTC AAAGGAGGAG TCATTGCAGT GTATCTTTCA
    GTTATCCTCG TCGTTGGAAG AGGTCTGGTT CGTGGAATCT TCACCACATC TCCAAGCACT
    GTCATGTTCA CAGAATTACC GAATGCGGAT CATCTTCTCA AAATTTGTCT CGACATCTAT
    TTGGTTCGAG AAGCGAAGGA CTTCATGTTG GAGCAGGATC TCTTCGCCAA ATTAATATTC
    CTGTTCCGAT CACCAGCGAC ACTCATCGAA TGGACACGAA TGAGCAAAAA GAAGCAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342183
    Clone ID Related Accession (Same CDS sequence) NM_001268459.3 , NM_001268459.2
    Accession Version NM_001268459.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268459.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACTA CAGCACCCGG AGATAAGGAA TCTGCTGCTT CGAAAGGAAT GATTGCAGTC
    ATGACATTTG TCATTTTCCA TTCGTATTCA ATCGCGCTGA CTGCTATGAT GACATGGGCT
    CTCCTCTATC ATTCGATATT CGGTCTCATT CTCCTCATCC TCACATGTAT CTTGTGGATC
    TTCCGTGATA CGAGAAAGTC ATCGTTTGCA ATGGCCCCTA TTATTTTGAT GTATATCGAG
    TTTCTATTGA TTCTTCAATA TTTCCTCAGT ATGGATATTC ATGCAGAAAT TGGCGATCCA
    GCGTGGATGA ACTTTGTTGG AATCGAATGG ACTACTCTTC CAGTACATGC TGTCATCATT
    CTCTGTGTCC AGACTCTTCT CACTCTCCCA GTGTTCTTGC TTCTACGTTT GGCTCGCAGG
    GAGAAGTTTT ACGAATCGCT GAGTGATTAT GAACGACAAC GACGAATCAA CTCATATGGA
    ACATTTGGAG CATCAAAGAC TGGAGCTGGG GGTGTTGCAG TGGCTAAGGA TCCAAAAAGT
    CGGAAGTTCG CGGCATTTGT GGAATATCTG TCGAATAAAG TGTCTGTCTA CTTCATATTT
    GTAGTATCAG TGGTACTTTT GGTGGTCTCT ACGTGTTTTG CTCCGAATTT CTACAATATT
    CTATTCTTCG CACTATGGGC ATTGAACTTG ATTTATCTCA AGTTCTCATT CCGTCTGTAT
    CGTGGATTGG CATATGCATT CTGGCTAACT CTCACGTTCT ACACTTCAAT TGTTATTATC
    GCCCTCTACA TTTATCAGTT CCCTGGAGTA TCTCAATGGA TTATTCGAAA TACTAGTTTG
    TCGCAGGAGT GGCTGAATGC AATTGGACTT GTCGATTTTA GAGCTATCGG TGAATCGGGA
    GCCCTATTTC TACAATTACT CGCCCCAATT GCCTTGTTCG TCGTCACAAT GCTCCAACTC
    AAGTTTTTCC ATGGACCATG GAGTCGAGCA ACGTCACCAA GAAGAGCAGA AAATGATCCA
    CCTACAAGTA CGACGGAGGC TGCGGCGGTA GCAAGCACAT CTGGAACACA AGGAAGAGCA
    CATGCTGCAG GCGATACCCT TGTCAAGAAG CTGCACAAAT TGGCAAATCA GACAATCGAA
    CTGTTATGGA GGTTTTTCGA AGTTCACATT TCGAAAATTG TCTTTGTCAT CATTGCGATT
    TTTATTGCCA ACAATATCAA TGCTCTATAC ATTCCACTTG TTATTCTCCT CTCATTGGCT
    ATATGTCTAC CATCAGCTGC AGATGGGATA TTCTCTTTAT TTATGTGTGC CTACTTATTC
    CTCGTGGCAC TGAGCAAGAT GATCTACCAA CTGGATATTG TTCCCGAGTT ATCACAAATC
    GACCGAGGAG TTGGAGCAGA TAACTGTTCA CATGGAAATA TTTCAATGCC TGAATGGTTT
    GGATTGAAGA AGGAAGTTGA AGGAACTGAA CCAATTTATA TGCTCTTTGG AGTCATTGTT
    TCGATTATTG CATTGGCTTT TCAATCAATT GTTATATATC GACAAAGACA TTATCGAGCT
    TCACTGGGAC TTCCTGAATC GATGCGAGCT AAAGTGTTCC CGGACTTCCA TCATTCTCAT
    TTTGATAGAT CTCTCAAAAA TGCCATCCAA TTCTTGATAG ACTATGGTTT CTATAAGTTT
    GGACTTGAAA TCACGATGAT CGCCATTGGA ATTGACATTT TCAATCGAAT GGATGCATTG
    GCAGCGATTC AATGTTTCTG GCTTGTCCTG TTTGCGCTCA ATAAAAGAGT CTTTGTTCGA
    AGAATATGGG TATTCTATGT GATCTATATG GCGATTCTGT ATCCACTTCA GTTCTTTTCA
    TATGTTGGAT TACCACCTGA CAGTTGTATT GAGTATCCAT GGTCGTATTG GATTCCATCG
    TATAGTGATG ATGCGAGATT CAATCTGTCG TATCTTCTGA ACTTGTCAAT ATACGGTGTA
    AACTGGCCAT CAGCATATCT CATTGGCGAT TTCTTCGTAC TCCTCTTGGC ATCATGCCAA
    TTAGCAGTTT TCCGGCGAGA AGGAGAAGAT AATGATTCAA TTTACAATGA TGGAAACTTT
    GTGATTAAGC CAGAAAATCC ACAATATGAC TTTATTGACA CCAAAAAGAG TTACGTTGAT
    TATTTCAAAT CATTCGTTTT CCATTATGGA CACTGGATTA CATTGATGTC TACACTAGCA
    GCAGGAATTG CTGGAACGTC ACTTTTTGCT TTGGGATACA TTATATTCAC TCTGACAATG
    CTGTGGTCTG GTAACAATTT GTATGTGATG AACTCAACTC TACGATCATT TGAACATACT
    TTGAAACGAT GGAATGCACT TTTGGGATAC ACATTGTTCA CGATTACAAT GAAAGTTTGC
    CTTCAAATAT TTGGATGTGT ATTCCTGTCG TGGTTCGATC AATCTGGTGG ATGGGGAAAG
    ACTCTGTGCA TCGTTCGTCA ACTCTTCAGT ATCACATGTG TTAATAACGA ATGTCATGTG
    CTCAAAGAGC TGGAGGATTT CTCGAAGGCG TGTGCTGTTG AGACGAAAGA AGGAAATATT
    GGATTTGATG TGATTGCGCT ATCCTTCCTC GTCTTCCAAA TCCGAATTTT CCATTCTTGG
    TATTTCCAAC ACTGCATGGT TGAATATCGA AGTGAGGTTA TTCTGGCCAA TAGAGGTGCC
    GTGCTGAAGA ATCAACTGAT TGAAAAGGAA ATGAAGGAAC AAAACGAGCA GCAAAAAGCC
    AAGTTCAATG ATATCAGAAG AAGAACTGAA GCTATTCGTG AAAGATATCA AAAACAAATC
    GAACGAGGAG CTGCTGAACG TGACTTCGAG CCGGTTACTT ATGGGCACGC CAAACGAGCG
    GGAGACTACT ACATGTTCAA ATACGATCCT GAAAATGATG ATCTTGTGGA ACCCGTCGAC
    AGTTTTGTAC CAGAAGTTGA TCCCAAGGCA ACTGCATATG ATCGACTTGA TCCTGGACAG
    ATAATGTACG CTGCCACCGC TCACGACCTC GACCTCGCCA AAACTGTACA GCAAGTCAAG
    AAAGGCGATA CTATCAAAGA TCCTGATTCA CGTGCTCTTA TCGCTGTTTC TGAACCAGAA
    GCTCGTAAGC CAGGAGGCAC TGAAGAAACG GATGGTGATG AAGATGAGGA TAATAAGGAT
    TCGAAAGTGG AAAGTACTGC TAAATTCATT CAAAAAATGA TCGCTTCTGC CTTGGACTTG
    TGTAGTGTAA CACTAAACAA ACTGTGCAGA GAACACCGAT ATGTTGGATT TGTTTTGAGC
    AAGGAAAAAC AAAAGTTGAA GTCAGGACAC TCGGAATCTC TTTCAAATAC ATCTAGAAAG
    CTGACGGATA TTCGATCTGC AGTTGATCTA CCAAGTCTTC AACTTGTGCA ATCGGCGAAT
    GATGTTGAAA AGATGGAGAC AGCCGTCAGC GTGGATTGGC AACAAAAATC ATCAGCAACG
    AGACTATTGA ATGCAGTTGT GAATTGTATT GGAGCACACA CTGATATTCT CTGCTACTTC
    TTCGCAATTA TGACACAAGT GATGACTGGT GGACTTATTA CTCTTCCACT TCCTCTGATG
    TCACTATTCT GGGGAAATCT ATCGAATCCT CGTCCATCCA AGTTCTTCTG GGTCACAATG
    ATCACCTACA CTGAGTGTGT CATTGTTATC AAGTTTGTTT GCCAATTTGC ATTCATGCCT
    TACAATTCTA TCACGTGGCG AACAGAGCAT CAAATGGACC CGATGTCATT GGATAAGTTA
    TTCGGAGTAT CACAGAGAGA TAGTTTTGCA CTTTGGGACA TTGTTTTGCT CTTCTCACTC
    TTCTTCCATC GGTATATGCT CAGAAAGTTA GGGTTATGGA AGGATGCCAA TTTGACGGAC
    ACGTTCACTT TGAAGGAAGA GCCTCGATCG GCGTCAGGAT CGGACACAGG ATCACCCAAG
    AAAATTGCTC AAGAGCCAAA GGTTGTTGTA ACTCAGTCAG ATACCCTTGA AGGTACAAGT
    GGAGGAGAGA TAGTCATACC ATCTGATCCA AATGCAGTTT CCAATATGGA AGAATTAGAC
    TGCGAACCTC CGATTCCAGA GAAGCAAAGT GGACCAATTG GAAGATTCAT TCATCAATTA
    TTCCATCCAA AATTCCGTTA CATTCGTGAT TTGTATCCAA TAATGTTTGG AATCGATGTG
    ATATGCTTCC TCATCATGAC ATTTGGATAC TCGGCATTTG GTGAAGGAGG TTCTGGAAAT
    GTTTTAGATG ATGTGAAGGC TTCTCGTATT CCGGTAACAC TTGTTGTTAT GCTCGTTGGA
    ATGACTCTGG CAATTATCAT TGATCGAGCA CTCTACCTCA GAAAATCAGT TGTCGGAAAG
    CTAATCTATC AAGTCTTAAT GATTGCTTTC CTTCACATTT GGGTATTCCT TGTTCTTCCA
    AATATGACTC GTCGTTCTGC GATATCGAAT CACGTTGCTC AAGCATTGTA CGTCATCAAA
    TCGTGCTATT TCCTTGTGTC TGCTTGGCAG ATCCGAAATG GTTATCCTGA ACTTTGTATT
    GGAAATCTAT TAACACATTC ATACGGAATG ACCAATATGA TTGCATTCAA AGTATTCATG
    AATATTCCAT TCCTCTTCGA GCTTCGTACA GCTATTGATT GGACATGGAC AGATACTTCA
    ATGCCTCTTT TCGATTTCTT CAATATGGAA AATTTCTATG CTCATATATT CAATATCAAG
    TGTGCCCGCC AGTTCGAAGC TGCCTATCCA GCTCCAAGAG GGATTCCCAA AGGAAAGTTG
    GTCAAGTATA TGATGGGATT CCCAATTATC ATTGGAGTCG TTATCTTCAT CTTTTCTCCT
    CTATTGCTCT GGTCGCTACT CAATCAAATC GGAACTATCA GTATGCCAGA AAAAGTTACG
    TTGAGGATTT CGATTGAAGG ATATCCACCA CTTTACGAGA TGGAGGCACA AGGAAGTAAT
    CACGATAACG CTGAACTTGG AATGATAAAA CCTGATCAAT TGGCATCTTT GAATCAAGCA
    TTGACGGATA GTTACACGAC ACGAGACACT AATTCTATTT TAAGATCAAG AATGTCTGTA
    TCATATCTGA AAGGATACAC ATATGAGGAT ATTCTGATTG TGCGATTCCG TCCAGAATCT
    GAAATTTATT GGCCGATTTC ACAGGATTCG AGAAATGCGA TGATCGATAA ATTGAGCCGA
    AACACGTCAG TCAATTTTGA AGTATCTCTG GAATTCACTC GGCCATATGA TCCAAATGAA
    AACGCTGCTT TAAAACATTC GAAATCGTGG CTTGTTCCAA TCTCCCTGGA TATGACGATT
    CGAGCAAAAA TTCAAAGTGC TCTCCGAGGA GATCCAGGTC ATCCGATTCT CATTCCACAG
    TCAATTCCTG CGTTTATTCA AGTTCCAAAT CAAGGAGAAT TGACCCTTCC AACATCAATC
    GGAAATACTA TTATCAATGA CGGAAATCCA CGGATTAATA CGACCGGGAT GGAAAAATCA
    GACGAAGCTC GGGCTTGGTT CGACTCGTTA ACCTTGAATC TCGAACAAGG AAAATCGCAA
    AACGAGAAAA TGTGGATCGC CACATCCGAA CATCCAGGAG ACCAAAATGC GAAACTCTGG
    ATAAAGACTG CAAATACGAC GTATTCGGGA AGGCCGTACC TTCAAGTTGT CGGATTTATT
    GATAGAGCAT TCCCATCATT TTTGGCGAAA GTTTTCAAAG GAGGAGTCAT TGCAGTGTAT
    CTTTCAGTTA TCCTCGTCGT TGGAAGAGGT CTGGTTCGTG GAATCTTCAC CACATCTCCA
    AGCACTGTCA TGTTCACAGA ATTACCGAAT GCGGATCATC TTCTCAAAAT TTGTCTCGAC
    ATCTATTTGG TTCGAGAAGC GAAGGACTTC ATGTTGGAGC AGGATCTCTT CGCCAAATTA
    ATATTCCTGT TCCGATCACC AGCGACACTC ATCGAATGGA CACGAATGAG CAAAAAGAAG
    CAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255388.2
    CDS57..7265
    Translation

    Target ORF information:

    RefSeq Version NM_001268459.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268459.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACTA CAGCACCCGG AGATAAGGAA TCTGCTGCTT CGAAAGGAAT GATTGCAGTC
    ATGACATTTG TCATTTTCCA TTCGTATTCA ATCGCGCTGA CTGCTATGAT GACATGGGCT
    CTCCTCTATC ATTCGATATT CGGTCTCATT CTCCTCATCC TCACATGTAT CTTGTGGATC
    TTCCGTGATA CGAGAAAGTC ATCGTTTGCA ATGGCCCCTA TTATTTTGAT GTATATCGAG
    TTTCTATTGA TTCTTCAATA TTTCCTCAGT ATGGATATTC ATGCAGAAAT TGGCGATCCA
    GCGTGGATGA ACTTTGTTGG AATCGAATGG ACTACTCTTC CAGTACATGC TGTCATCATT
    CTCTGTGTCC AGACTCTTCT CACTCTCCCA GTGTTCTTGC TTCTACGTTT GGCTCGCAGG
    GAGAAGTTTT ACGAATCGCT GAGTGATTAT GAACGACAAC GACGAATCAA CTCATATGGA
    ACATTTGGAG CATCAAAGAC TGGAGCTGGG GGTGTTGCAG TGGCTAAGGA TCCAAAAAGT
    CGGAAGTTCG CGGCATTTGT GGAATATCTG TCGAATAAAG TGTCTGTCTA CTTCATATTT
    GTAGTATCAG TGGTACTTTT GGTGGTCTCT ACGTGTTTTG CTCCGAATTT CTACAATATT
    CTATTCTTCG CACTATGGGC ATTGAACTTG ATTTATCTCA AGTTCTCATT CCGTCTGTAT
    CGTGGATTGG CATATGCATT CTGGCTAACT CTCACGTTCT ACACTTCAAT TGTTATTATC
    GCCCTCTACA TTTATCAGTT CCCTGGAGTA TCTCAATGGA TTATTCGAAA TACTAGTTTG
    TCGCAGGAGT GGCTGAATGC AATTGGACTT GTCGATTTTA GAGCTATCGG TGAATCGGGA
    GCCCTATTTC TACAATTACT CGCCCCAATT GCCTTGTTCG TCGTCACAAT GCTCCAACTC
    AAGTTTTTCC ATGGACCATG GAGTCGAGCA ACGTCACCAA GAAGAGCAGA AAATGATCCA
    CCTACAAGTA CGACGGAGGC TGCGGCGGTA GCAAGCACAT CTGGAACACA AGGAAGAGCA
    CATGCTGCAG GCGATACCCT TGTCAAGAAG CTGCACAAAT TGGCAAATCA GACAATCGAA
    CTGTTATGGA GGTTTTTCGA AGTTCACATT TCGAAAATTG TCTTTGTCAT CATTGCGATT
    TTTATTGCCA ACAATATCAA TGCTCTATAC ATTCCACTTG TTATTCTCCT CTCATTGGCT
    ATATGTCTAC CATCAGCTGC AGATGGGATA TTCTCTTTAT TTATGTGTGC CTACTTATTC
    CTCGTGGCAC TGAGCAAGAT GATCTACCAA CTGGATATTG TTCCCGAGTT ATCACAAATC
    GACCGAGGAG TTGGAGCAGA TAACTGTTCA CATGGAAATA TTTCAATGCC TGAATGGTTT
    GGATTGAAGA AGGAAGTTGA AGGAACTGAA CCAATTTATA TGCTCTTTGG AGTCATTGTT
    TCGATTATTG CATTGGCTTT TCAATCAATT GTTATATATC GACAAAGACA TTATCGAGCT
    TCACTGGGAC TTCCTGAATC GATGCGAGCT AAAGTGTTCC CGGACTTCCA TCATTCTCAT
    TTTGATAGAT CTCTCAAAAA TGCCATCCAA TTCTTGATAG ACTATGGTTT CTATAAGTTT
    GGACTTGAAA TCACGATGAT CGCCATTGGA ATTGACATTT TCAATCGAAT GGATGCATTG
    GCAGCGATTC AATGTTTCTG GCTTGTCCTG TTTGCGCTCA ATAAAAGAGT CTTTGTTCGA
    AGAATATGGG TATTCTATGT GATCTATATG GCGATTCTGT ATCCACTTCA GTTCTTTTCA
    TATGTTGGAT TACCACCTGA CAGTTGTATT GAGTATCCAT GGTCGTATTG GATTCCATCG
    TATAGTGATG ATGCGAGATT CAATCTGTCG TATCTTCTGA ACTTGTCAAT ATACGGTGTA
    AACTGGCCAT CAGCATATCT CATTGGCGAT TTCTTCGTAC TCCTCTTGGC ATCATGCCAA
    TTAGCAGTTT TCCGGCGAGA AGGAGAAGAT AATGATTCAA TTTACAATGA TGGAAACTTT
    GTGATTAAGC CAGAAAATCC ACAATATGAC TTTATTGACA CCAAAAAGAG TTACGTTGAT
    TATTTCAAAT CATTCGTTTT CCATTATGGA CACTGGATTA CATTGATGTC TACACTAGCA
    GCAGGAATTG CTGGAACGTC ACTTTTTGCT TTGGGATACA TTATATTCAC TCTGACAATG
    CTGTGGTCTG GTAACAATTT GTATGTGATG AACTCAACTC TACGATCATT TGAACATACT
    TTGAAACGAT GGAATGCACT TTTGGGATAC ACATTGTTCA CGATTACAAT GAAAGTTTGC
    CTTCAAATAT TTGGATGTGT ATTCCTGTCG TGGTTCGATC AATCTGGTGG ATGGGGAAAG
    ACTCTGTGCA TCGTTCGTCA ACTCTTCAGT ATCACATGTG TTAATAACGA ATGTCATGTG
    CTCAAAGAGC TGGAGGATTT CTCGAAGGCG TGTGCTGTTG AGACGAAAGA AGGAAATATT
    GGATTTGATG TGATTGCGCT ATCCTTCCTC GTCTTCCAAA TCCGAATTTT CCATTCTTGG
    TATTTCCAAC ACTGCATGGT TGAATATCGA AGTGAGGTTA TTCTGGCCAA TAGAGGTGCC
    GTGCTGAAGA ATCAACTGAT TGAAAAGGAA ATGAAGGAAC AAAACGAGCA GCAAAAAGCC
    AAGTTCAATG ATATCAGAAG AAGAACTGAA GCTATTCGTG AAAGATATCA AAAACAAATC
    GAACGAGGAG CTGCTGAACG TGACTTCGAG CCGGTTACTT ATGGGCACGC CAAACGAGCG
    GGAGACTACT ACATGTTCAA ATACGATCCT GAAAATGATG ATCTTGTGGA ACCCGTCGAC
    AGTTTTGTAC CAGAAGTTGA TCCCAAGGCA ACTGCATATG ATCGACTTGA TCCTGGACAG
    ATAATGTACG CTGCCACCGC TCACGACCTC GACCTCGCCA AAACTGTACA GCAAGTCAAG
    AAAGGCGATA CTATCAAAGA TCCTGATTCA CGTGCTCTTA TCGCTGTTTC TGAACCAGAA
    GCTCGTAAGC CAGGAGGCAC TGAAGAAACG GATGGTGATG AAGATGAGGA TAATAAGGAT
    TCGAAAGTGG AAAGTACTGC TAAATTCATT CAAAAAATGA TCGCTTCTGC CTTGGACTTG
    TGTAGTGTAA CACTAAACAA ACTGTGCAGA GAACACCGAT ATGTTGGATT TGTTTTGAGC
    AAGGAAAAAC AAAAGTTGAA GTCAGGACAC TCGGAATCTC TTTCAAATAC ATCTAGAAAG
    CTGACGGATA TTCGATCTGC AGTTGATCTA CCAAGTCTTC AACTTGTGCA ATCGGCGAAT
    GATGTTGAAA AGATGGAGAC AGCCGTCAGC GTGGATTGGC AACAAAAATC ATCAGCAACG
    AGACTATTGA ATGCAGTTGT GAATTGTATT GGAGCACACA CTGATATTCT CTGCTACTTC
    TTCGCAATTA TGACACAAGT GATGACTGGT GGACTTATTA CTCTTCCACT TCCTCTGATG
    TCACTATTCT GGGGAAATCT ATCGAATCCT CGTCCATCCA AGTTCTTCTG GGTCACAATG
    ATCACCTACA CTGAGTGTGT CATTGTTATC AAGTTTGTTT GCCAATTTGC ATTCATGCCT
    TACAATTCTA TCACGTGGCG AACAGAGCAT CAAATGGACC CGATGTCATT GGATAAGTTA
    TTCGGAGTAT CACAGAGAGA TAGTTTTGCA CTTTGGGACA TTGTTTTGCT CTTCTCACTC
    TTCTTCCATC GGTATATGCT CAGAAAGTTA GGGTTATGGA AGGATGCCAA TTTGACGGAC
    ACGTTCACTT TGAAGGAAGA GCCTCGATCG GCGTCAGGAT CGGACACAGG ATCACCCAAG
    AAAATTGCTC AAGAGCCAAA GGTTGTTGTA ACTCAGTCAG ATACCCTTGA AGGTACAAGT
    GGAGGAGAGA TAGTCATACC ATCTGATCCA AATGCAGTTT CCAATATGGA AGAATTAGAC
    TGCGAACCTC CGATTCCAGA GAAGCAAAGT GGACCAATTG GAAGATTCAT TCATCAATTA
    TTCCATCCAA AATTCCGTTA CATTCGTGAT TTGTATCCAA TAATGTTTGG AATCGATGTG
    ATATGCTTCC TCATCATGAC ATTTGGATAC TCGGCATTTG GTGAAGGAGG TTCTGGAAAT
    GTTTTAGATG ATGTGAAGGC TTCTCGTATT CCGGTAACAC TTGTTGTTAT GCTCGTTGGA
    ATGACTCTGG CAATTATCAT TGATCGAGCA CTCTACCTCA GAAAATCAGT TGTCGGAAAG
    CTAATCTATC AAGTCTTAAT GATTGCTTTC CTTCACATTT GGGTATTCCT TGTTCTTCCA
    AATATGACTC GTCGTTCTGC GATATCGAAT CACGTTGCTC AAGCATTGTA CGTCATCAAA
    TCGTGCTATT TCCTTGTGTC TGCTTGGCAG ATCCGAAATG GTTATCCTGA ACTTTGTATT
    GGAAATCTAT TAACACATTC ATACGGAATG ACCAATATGA TTGCATTCAA AGTATTCATG
    AATATTCCAT TCCTCTTCGA GCTTCGTACA GCTATTGATT GGACATGGAC AGATACTTCA
    ATGCCTCTTT TCGATTTCTT CAATATGGAA AATTTCTATG CTCATATATT CAATATCAAG
    TGTGCCCGCC AGTTCGAAGC TGCCTATCCA GCTCCAAGAG GGATTCCCAA AGGAAAGTTG
    GTCAAGTATA TGATGGGATT CCCAATTATC ATTGGAGTCG TTATCTTCAT CTTTTCTCCT
    CTATTGCTCT GGTCGCTACT CAATCAAATC GGAACTATCA GTATGCCAGA AAAAGTTACG
    TTGAGGATTT CGATTGAAGG ATATCCACCA CTTTACGAGA TGGAGGCACA AGGAAGTAAT
    CACGATAACG CTGAACTTGG AATGATAAAA CCTGATCAAT TGGCATCTTT GAATCAAGCA
    TTGACGGATA GTTACACGAC ACGAGACACT AATTCTATTT TAAGATCAAG AATGTCTGTA
    TCATATCTGA AAGGATACAC ATATGAGGAT ATTCTGATTG TGCGATTCCG TCCAGAATCT
    GAAATTTATT GGCCGATTTC ACAGGATTCG AGAAATGCGA TGATCGATAA ATTGAGCCGA
    AACACGTCAG TCAATTTTGA AGTATCTCTG GAATTCACTC GGCCATATGA TCCAAATGAA
    AACGCTGCTT TAAAACATTC GAAATCGTGG CTTGTTCCAA TCTCCCTGGA TATGACGATT
    CGAGCAAAAA TTCAAAGTGC TCTCCGAGGA GATCCAGGTC ATCCGATTCT CATTCCACAG
    TCAATTCCTG CGTTTATTCA AGTTCCAAAT CAAGGAGAAT TGACCCTTCC AACATCAATC
    GGAAATACTA TTATCAATGA CGGAAATCCA CGGATTAATA CGACCGGGAT GGAAAAATCA
    GACGAAGCTC GGGCTTGGTT CGACTCGTTA ACCTTGAATC TCGAACAAGG AAAATCGCAA
    AACGAGAAAA TGTGGATCGC CACATCCGAA CATCCAGGAG ACCAAAATGC GAAACTCTGG
    ATAAAGACTG CAAATACGAC GTATTCGGGA AGGCCGTACC TTCAAGTTGT CGGATTTATT
    GATAGAGCAT TCCCATCATT TTTGGCGAAA GTTTTCAAAG GAGGAGTCAT TGCAGTGTAT
    CTTTCAGTTA TCCTCGTCGT TGGAAGAGGT CTGGTTCGTG GAATCTTCAC CACATCTCCA
    AGCACTGTCA TGTTCACAGA ATTACCGAAT GCGGATCATC TTCTCAAAAT TTGTCTCGAC
    ATCTATTTGG TTCGAGAAGC GAAGGACTTC ATGTTGGAGC AGGATCTCTT CGCCAAATTA
    ATATTCCTGT TCCGATCACC AGCGACACTC ATCGAATGGA CACGAATGAG CAAAAAGAAG
    CAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342183
    Clone ID Related Accession (Same CDS sequence) NM_001268459.3 , NM_001268459.2
    Accession Version NM_001268459.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Apr 20, 2015 this sequence version replaced NM_001268459.1. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACTA CAGCACCCGG AGATAAGGAA TCTGCTGCTT CGAAAGGAAT GATTGCAGTC
    ATGACATTTG TCATTTTCCA TTCGTATTCA ATCGCGCTGA CTGCTATGAT GACATGGGCT
    CTCCTCTATC ATTCGATATT CGGTCTCATT CTCCTCATCC TCACATGTAT CTTGTGGATC
    TTCCGTGATA CGAGAAAGTC ATCGTTTGCA ATGGCCCCTA TTATTTTGAT GTATATCGAG
    TTTCTATTGA TTCTTCAATA TTTCCTCAGT ATGGATATTC ATGCAGAAAT TGGCGATCCA
    GCGTGGATGA ACTTTGTTGG AATCGAATGG ACTACTCTTC CAGTACATGC TGTCATCATT
    CTCTGTGTCC AGACTCTTCT CACTCTCCCA GTGTTCTTGC TTCTACGTTT GGCTCGCAGG
    GAGAAGTTTT ACGAATCGCT GAGTGATTAT GAACGACAAC GACGAATCAA CTCATATGGA
    ACATTTGGAG CATCAAAGAC TGGAGCTGGG GGTGTTGCAG TGGCTAAGGA TCCAAAAAGT
    CGGAAGTTCG CGGCATTTGT GGAATATCTG TCGAATAAAG TGTCTGTCTA CTTCATATTT
    GTAGTATCAG TGGTACTTTT GGTGGTCTCT ACGTGTTTTG CTCCGAATTT CTACAATATT
    CTATTCTTCG CACTATGGGC ATTGAACTTG ATTTATCTCA AGTTCTCATT CCGTCTGTAT
    CGTGGATTGG CATATGCATT CTGGCTAACT CTCACGTTCT ACACTTCAAT TGTTATTATC
    GCCCTCTACA TTTATCAGTT CCCTGGAGTA TCTCAATGGA TTATTCGAAA TACTAGTTTG
    TCGCAGGAGT GGCTGAATGC AATTGGACTT GTCGATTTTA GAGCTATCGG TGAATCGGGA
    GCCCTATTTC TACAATTACT CGCCCCAATT GCCTTGTTCG TCGTCACAAT GCTCCAACTC
    AAGTTTTTCC ATGGACCATG GAGTCGAGCA ACGTCACCAA GAAGAGCAGA AAATGATCCA
    CCTACAAGTA CGACGGAGGC TGCGGCGGTA GCAAGCACAT CTGGAACACA AGGAAGAGCA
    CATGCTGCAG GCGATACCCT TGTCAAGAAG CTGCACAAAT TGGCAAATCA GACAATCGAA
    CTGTTATGGA GGTTTTTCGA AGTTCACATT TCGAAAATTG TCTTTGTCAT CATTGCGATT
    TTTATTGCCA ACAATATCAA TGCTCTATAC ATTCCACTTG TTATTCTCCT CTCATTGGCT
    ATATGTCTAC CATCAGCTGC AGATGGGATA TTCTCTTTAT TTATGTGTGC CTACTTATTC
    CTCGTGGCAC TGAGCAAGAT GATCTACCAA CTGGATATTG TTCCCGAGTT ATCACAAATC
    GACCGAGGAG TTGGAGCAGA TAACTGTTCA CATGGAAATA TTTCAATGCC TGAATGGTTT
    GGATTGAAGA AGGAAGTTGA AGGAACTGAA CCAATTTATA TGCTCTTTGG AGTCATTGTT
    TCGATTATTG CATTGGCTTT TCAATCAATT GTTATATATC GACAAAGACA TTATCGAGCT
    TCACTGGGAC TTCCTGAATC GATGCGAGCT AAAGTGTTCC CGGACTTCCA TCATTCTCAT
    TTTGATAGAT CTCTCAAAAA TGCCATCCAA TTCTTGATAG ACTATGGTTT CTATAAGTTT
    GGACTTGAAA TCACGATGAT CGCCATTGGA ATTGACATTT TCAATCGAAT GGATGCATTG
    GCAGCGATTC AATGTTTCTG GCTTGTCCTG TTTGCGCTCA ATAAAAGAGT CTTTGTTCGA
    AGAATATGGG TATTCTATGT GATCTATATG GCGATTCTGT ATCCACTTCA GTTCTTTTCA
    TATGTTGGAT TACCACCTGA CAGTTGTATT GAGTATCCAT GGTCGTATTG GATTCCATCG
    TATAGTGATG ATGCGAGATT CAATCTGTCG TATCTTCTGA ACTTGTCAAT ATACGGTGTA
    AACTGGCCAT CAGCATATCT CATTGGCGAT TTCTTCGTAC TCCTCTTGGC ATCATGCCAA
    TTAGCAGTTT TCCGGCGAGA AGGAGAAGAT AATGATTCAA TTTACAATGA TGGAAACTTT
    GTGATTAAGC CAGAAAATCC ACAATATGAC TTTATTGACA CCAAAAAGAG TTACGTTGAT
    TATTTCAAAT CATTCGTTTT CCATTATGGA CACTGGATTA CATTGATGTC TACACTAGCA
    GCAGGAATTG CTGGAACGTC ACTTTTTGCT TTGGGATACA TTATATTCAC TCTGACAATG
    CTGTGGTCTG GTAACAATTT GTATGTGATG AACTCAACTC TACGATCATT TGAACATACT
    TTGAAACGAT GGAATGCACT TTTGGGATAC ACATTGTTCA CGATTACAAT GAAAGTTTGC
    CTTCAAATAT TTGGATGTGT ATTCCTGTCG TGGTTCGATC AATCTGGTGG ATGGGGAAAG
    ACTCTGTGCA TCGTTCGTCA ACTCTTCAGT ATCACATGTG TTAATAACGA ATGTCATGTG
    CTCAAAGAGC TGGAGGATTT CTCGAAGGCG TGTGCTGTTG AGACGAAAGA AGGAAATATT
    GGATTTGATG TGATTGCGCT ATCCTTCCTC GTCTTCCAAA TCCGAATTTT CCATTCTTGG
    TATTTCCAAC ACTGCATGGT TGAATATCGA AGTGAGGTTA TTCTGGCCAA TAGAGGTGCC
    GTGCTGAAGA ATCAACTGAT TGAAAAGGAA ATGAAGGAAC AAAACGAGCA GCAAAAAGCC
    AAGTTCAATG ATATCAGAAG AAGAACTGAA GCTATTCGTG AAAGATATCA AAAACAAATC
    GAACGAGGAG CTGCTGAACG TGACTTCGAG CCGGTTACTT ATGGGCACGC CAAACGAGCG
    GGAGACTACT ACATGTTCAA ATACGATCCT GAAAATGATG ATCTTGTGGA ACCCGTCGAC
    AGTTTTGTAC CAGAAGTTGA TCCCAAGGCA ACTGCATATG ATCGACTTGA TCCTGGACAG
    ATAATGTACG CTGCCACCGC TCACGACCTC GACCTCGCCA AAACTGTACA GCAAGTCAAG
    AAAGGCGATA CTATCAAAGA TCCTGATTCA CGTGCTCTTA TCGCTGTTTC TGAACCAGAA
    GCTCGTAAGC CAGGAGGCAC TGAAGAAACG GATGGTGATG AAGATGAGGA TAATAAGGAT
    TCGAAAGTGG AAAGTACTGC TAAATTCATT CAAAAAATGA TCGCTTCTGC CTTGGACTTG
    TGTAGTGTAA CACTAAACAA ACTGTGCAGA GAACACCGAT ATGTTGGATT TGTTTTGAGC
    AAGGAAAAAC AAAAGTTGAA GTCAGGACAC TCGGAATCTC TTTCAAATAC ATCTAGAAAG
    CTGACGGATA TTCGATCTGC AGTTGATCTA CCAAGTCTTC AACTTGTGCA ATCGGCGAAT
    GATGTTGAAA AGATGGAGAC AGCCGTCAGC GTGGATTGGC AACAAAAATC ATCAGCAACG
    AGACTATTGA ATGCAGTTGT GAATTGTATT GGAGCACACA CTGATATTCT CTGCTACTTC
    TTCGCAATTA TGACACAAGT GATGACTGGT GGACTTATTA CTCTTCCACT TCCTCTGATG
    TCACTATTCT GGGGAAATCT ATCGAATCCT CGTCCATCCA AGTTCTTCTG GGTCACAATG
    ATCACCTACA CTGAGTGTGT CATTGTTATC AAGTTTGTTT GCCAATTTGC ATTCATGCCT
    TACAATTCTA TCACGTGGCG AACAGAGCAT CAAATGGACC CGATGTCATT GGATAAGTTA
    TTCGGAGTAT CACAGAGAGA TAGTTTTGCA CTTTGGGACA TTGTTTTGCT CTTCTCACTC
    TTCTTCCATC GGTATATGCT CAGAAAGTTA GGGTTATGGA AGGATGCCAA TTTGACGGAC
    ACGTTCACTT TGAAGGAAGA GCCTCGATCG GCGTCAGGAT CGGACACAGG ATCACCCAAG
    AAAATTGCTC AAGAGCCAAA GGTTGTTGTA ACTCAGTCAG ATACCCTTGA AGGTACAAGT
    GGAGGAGAGA TAGTCATACC ATCTGATCCA AATGCAGTTT CCAATATGGA AGAATTAGAC
    TGCGAACCTC CGATTCCAGA GAAGCAAAGT GGACCAATTG GAAGATTCAT TCATCAATTA
    TTCCATCCAA AATTCCGTTA CATTCGTGAT TTGTATCCAA TAATGTTTGG AATCGATGTG
    ATATGCTTCC TCATCATGAC ATTTGGATAC TCGGCATTTG GTGAAGGAGG TTCTGGAAAT
    GTTTTAGATG ATGTGAAGGC TTCTCGTATT CCGGTAACAC TTGTTGTTAT GCTCGTTGGA
    ATGACTCTGG CAATTATCAT TGATCGAGCA CTCTACCTCA GAAAATCAGT TGTCGGAAAG
    CTAATCTATC AAGTCTTAAT GATTGCTTTC CTTCACATTT GGGTATTCCT TGTTCTTCCA
    AATATGACTC GTCGTTCTGC GATATCGAAT CACGTTGCTC AAGCATTGTA CGTCATCAAA
    TCGTGCTATT TCCTTGTGTC TGCTTGGCAG ATCCGAAATG GTTATCCTGA ACTTTGTATT
    GGAAATCTAT TAACACATTC ATACGGAATG ACCAATATGA TTGCATTCAA AGTATTCATG
    AATATTCCAT TCCTCTTCGA GCTTCGTACA GCTATTGATT GGACATGGAC AGATACTTCA
    ATGCCTCTTT TCGATTTCTT CAATATGGAA AATTTCTATG CTCATATATT CAATATCAAG
    TGTGCCCGCC AGTTCGAAGC TGCCTATCCA GCTCCAAGAG GGATTCCCAA AGGAAAGTTG
    GTCAAGTATA TGATGGGATT CCCAATTATC ATTGGAGTCG TTATCTTCAT CTTTTCTCCT
    CTATTGCTCT GGTCGCTACT CAATCAAATC GGAACTATCA GTATGCCAGA AAAAGTTACG
    TTGAGGATTT CGATTGAAGG ATATCCACCA CTTTACGAGA TGGAGGCACA AGGAAGTAAT
    CACGATAACG CTGAACTTGG AATGATAAAA CCTGATCAAT TGGCATCTTT GAATCAAGCA
    TTGACGGATA GTTACACGAC ACGAGACACT AATTCTATTT TAAGATCAAG AATGTCTGTA
    TCATATCTGA AAGGATACAC ATATGAGGAT ATTCTGATTG TGCGATTCCG TCCAGAATCT
    GAAATTTATT GGCCGATTTC ACAGGATTCG AGAAATGCGA TGATCGATAA ATTGAGCCGA
    AACACGTCAG TCAATTTTGA AGTATCTCTG GAATTCACTC GGCCATATGA TCCAAATGAA
    AACGCTGCTT TAAAACATTC GAAATCGTGG CTTGTTCCAA TCTCCCTGGA TATGACGATT
    CGAGCAAAAA TTCAAAGTGC TCTCCGAGGA GATCCAGGTC ATCCGATTCT CATTCCACAG
    TCAATTCCTG CGTTTATTCA AGTTCCAAAT CAAGGAGAAT TGACCCTTCC AACATCAATC
    GGAAATACTA TTATCAATGA CGGAAATCCA CGGATTAATA CGACCGGGAT GGAAAAATCA
    GACGAAGCTC GGGCTTGGTT CGACTCGTTA ACCTTGAATC TCGAACAAGG AAAATCGCAA
    AACGAGAAAA TGTGGATCGC CACATCCGAA CATCCAGGAG ACCAAAATGC GAAACTCTGG
    ATAAAGACTG CAAATACGAC GTATTCGGGA AGGCCGTACC TTCAAGTTGT CGGATTTATT
    GATAGAGCAT TCCCATCATT TTTGGCGAAA GTTTTCAAAG GAGGAGTCAT TGCAGTGTAT
    CTTTCAGTTA TCCTCGTCGT TGGAAGAGGT CTGGTTCGTG GAATCTTCAC CACATCTCCA
    AGCACTGTCA TGTTCACAGA ATTACCGAAT GCGGATCATC TTCTCAAAAT TTGTCTCGAC
    ATCTATTTGG TTCGAGAAGC GAAGGACTTC ATGTTGGAGC AGGATCTCTT CGCCAAATTA
    ATATTCCTGT TCCGATCACC AGCGACACTC ATCGAATGGA CACGAATGAG CAAAAAGAAG
    CAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255388.2
    CDS1..7209
    Translation

    Target ORF information:

    RefSeq Version NM_001268459.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268459.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACTA CAGCACCCGG AGATAAGGAA TCTGCTGCTT CGAAAGGAAT GATTGCAGTC
    ATGACATTTG TCATTTTCCA TTCGTATTCA ATCGCGCTGA CTGCTATGAT GACATGGGCT
    CTCCTCTATC ATTCGATATT CGGTCTCATT CTCCTCATCC TCACATGTAT CTTGTGGATC
    TTCCGTGATA CGAGAAAGTC ATCGTTTGCA ATGGCCCCTA TTATTTTGAT GTATATCGAG
    TTTCTATTGA TTCTTCAATA TTTCCTCAGT ATGGATATTC ATGCAGAAAT TGGCGATCCA
    GCGTGGATGA ACTTTGTTGG AATCGAATGG ACTACTCTTC CAGTACATGC TGTCATCATT
    CTCTGTGTCC AGACTCTTCT CACTCTCCCA GTGTTCTTGC TTCTACGTTT GGCTCGCAGG
    GAGAAGTTTT ACGAATCGCT GAGTGATTAT GAACGACAAC GACGAATCAA CTCATATGGA
    ACATTTGGAG CATCAAAGAC TGGAGCTGGG GGTGTTGCAG TGGCTAAGGA TCCAAAAAGT
    CGGAAGTTCG CGGCATTTGT GGAATATCTG TCGAATAAAG TGTCTGTCTA CTTCATATTT
    GTAGTATCAG TGGTACTTTT GGTGGTCTCT ACGTGTTTTG CTCCGAATTT CTACAATATT
    CTATTCTTCG CACTATGGGC ATTGAACTTG ATTTATCTCA AGTTCTCATT CCGTCTGTAT
    CGTGGATTGG CATATGCATT CTGGCTAACT CTCACGTTCT ACACTTCAAT TGTTATTATC
    GCCCTCTACA TTTATCAGTT CCCTGGAGTA TCTCAATGGA TTATTCGAAA TACTAGTTTG
    TCGCAGGAGT GGCTGAATGC AATTGGACTT GTCGATTTTA GAGCTATCGG TGAATCGGGA
    GCCCTATTTC TACAATTACT CGCCCCAATT GCCTTGTTCG TCGTCACAAT GCTCCAACTC
    AAGTTTTTCC ATGGACCATG GAGTCGAGCA ACGTCACCAA GAAGAGCAGA AAATGATCCA
    CCTACAAGTA CGACGGAGGC TGCGGCGGTA GCAAGCACAT CTGGAACACA AGGAAGAGCA
    CATGCTGCAG GCGATACCCT TGTCAAGAAG CTGCACAAAT TGGCAAATCA GACAATCGAA
    CTGTTATGGA GGTTTTTCGA AGTTCACATT TCGAAAATTG TCTTTGTCAT CATTGCGATT
    TTTATTGCCA ACAATATCAA TGCTCTATAC ATTCCACTTG TTATTCTCCT CTCATTGGCT
    ATATGTCTAC CATCAGCTGC AGATGGGATA TTCTCTTTAT TTATGTGTGC CTACTTATTC
    CTCGTGGCAC TGAGCAAGAT GATCTACCAA CTGGATATTG TTCCCGAGTT ATCACAAATC
    GACCGAGGAG TTGGAGCAGA TAACTGTTCA CATGGAAATA TTTCAATGCC TGAATGGTTT
    GGATTGAAGA AGGAAGTTGA AGGAACTGAA CCAATTTATA TGCTCTTTGG AGTCATTGTT
    TCGATTATTG CATTGGCTTT TCAATCAATT GTTATATATC GACAAAGACA TTATCGAGCT
    TCACTGGGAC TTCCTGAATC GATGCGAGCT AAAGTGTTCC CGGACTTCCA TCATTCTCAT
    TTTGATAGAT CTCTCAAAAA TGCCATCCAA TTCTTGATAG ACTATGGTTT CTATAAGTTT
    GGACTTGAAA TCACGATGAT CGCCATTGGA ATTGACATTT TCAATCGAAT GGATGCATTG
    GCAGCGATTC AATGTTTCTG GCTTGTCCTG TTTGCGCTCA ATAAAAGAGT CTTTGTTCGA
    AGAATATGGG TATTCTATGT GATCTATATG GCGATTCTGT ATCCACTTCA GTTCTTTTCA
    TATGTTGGAT TACCACCTGA CAGTTGTATT GAGTATCCAT GGTCGTATTG GATTCCATCG
    TATAGTGATG ATGCGAGATT CAATCTGTCG TATCTTCTGA ACTTGTCAAT ATACGGTGTA
    AACTGGCCAT CAGCATATCT CATTGGCGAT TTCTTCGTAC TCCTCTTGGC ATCATGCCAA
    TTAGCAGTTT TCCGGCGAGA AGGAGAAGAT AATGATTCAA TTTACAATGA TGGAAACTTT
    GTGATTAAGC CAGAAAATCC ACAATATGAC TTTATTGACA CCAAAAAGAG TTACGTTGAT
    TATTTCAAAT CATTCGTTTT CCATTATGGA CACTGGATTA CATTGATGTC TACACTAGCA
    GCAGGAATTG CTGGAACGTC ACTTTTTGCT TTGGGATACA TTATATTCAC TCTGACAATG
    CTGTGGTCTG GTAACAATTT GTATGTGATG AACTCAACTC TACGATCATT TGAACATACT
    TTGAAACGAT GGAATGCACT TTTGGGATAC ACATTGTTCA CGATTACAAT GAAAGTTTGC
    CTTCAAATAT TTGGATGTGT ATTCCTGTCG TGGTTCGATC AATCTGGTGG ATGGGGAAAG
    ACTCTGTGCA TCGTTCGTCA ACTCTTCAGT ATCACATGTG TTAATAACGA ATGTCATGTG
    CTCAAAGAGC TGGAGGATTT CTCGAAGGCG TGTGCTGTTG AGACGAAAGA AGGAAATATT
    GGATTTGATG TGATTGCGCT ATCCTTCCTC GTCTTCCAAA TCCGAATTTT CCATTCTTGG
    TATTTCCAAC ACTGCATGGT TGAATATCGA AGTGAGGTTA TTCTGGCCAA TAGAGGTGCC
    GTGCTGAAGA ATCAACTGAT TGAAAAGGAA ATGAAGGAAC AAAACGAGCA GCAAAAAGCC
    AAGTTCAATG ATATCAGAAG AAGAACTGAA GCTATTCGTG AAAGATATCA AAAACAAATC
    GAACGAGGAG CTGCTGAACG TGACTTCGAG CCGGTTACTT ATGGGCACGC CAAACGAGCG
    GGAGACTACT ACATGTTCAA ATACGATCCT GAAAATGATG ATCTTGTGGA ACCCGTCGAC
    AGTTTTGTAC CAGAAGTTGA TCCCAAGGCA ACTGCATATG ATCGACTTGA TCCTGGACAG
    ATAATGTACG CTGCCACCGC TCACGACCTC GACCTCGCCA AAACTGTACA GCAAGTCAAG
    AAAGGCGATA CTATCAAAGA TCCTGATTCA CGTGCTCTTA TCGCTGTTTC TGAACCAGAA
    GCTCGTAAGC CAGGAGGCAC TGAAGAAACG GATGGTGATG AAGATGAGGA TAATAAGGAT
    TCGAAAGTGG AAAGTACTGC TAAATTCATT CAAAAAATGA TCGCTTCTGC CTTGGACTTG
    TGTAGTGTAA CACTAAACAA ACTGTGCAGA GAACACCGAT ATGTTGGATT TGTTTTGAGC
    AAGGAAAAAC AAAAGTTGAA GTCAGGACAC TCGGAATCTC TTTCAAATAC ATCTAGAAAG
    CTGACGGATA TTCGATCTGC AGTTGATCTA CCAAGTCTTC AACTTGTGCA ATCGGCGAAT
    GATGTTGAAA AGATGGAGAC AGCCGTCAGC GTGGATTGGC AACAAAAATC ATCAGCAACG
    AGACTATTGA ATGCAGTTGT GAATTGTATT GGAGCACACA CTGATATTCT CTGCTACTTC
    TTCGCAATTA TGACACAAGT GATGACTGGT GGACTTATTA CTCTTCCACT TCCTCTGATG
    TCACTATTCT GGGGAAATCT ATCGAATCCT CGTCCATCCA AGTTCTTCTG GGTCACAATG
    ATCACCTACA CTGAGTGTGT CATTGTTATC AAGTTTGTTT GCCAATTTGC ATTCATGCCT
    TACAATTCTA TCACGTGGCG AACAGAGCAT CAAATGGACC CGATGTCATT GGATAAGTTA
    TTCGGAGTAT CACAGAGAGA TAGTTTTGCA CTTTGGGACA TTGTTTTGCT CTTCTCACTC
    TTCTTCCATC GGTATATGCT CAGAAAGTTA GGGTTATGGA AGGATGCCAA TTTGACGGAC
    ACGTTCACTT TGAAGGAAGA GCCTCGATCG GCGTCAGGAT CGGACACAGG ATCACCCAAG
    AAAATTGCTC AAGAGCCAAA GGTTGTTGTA ACTCAGTCAG ATACCCTTGA AGGTACAAGT
    GGAGGAGAGA TAGTCATACC ATCTGATCCA AATGCAGTTT CCAATATGGA AGAATTAGAC
    TGCGAACCTC CGATTCCAGA GAAGCAAAGT GGACCAATTG GAAGATTCAT TCATCAATTA
    TTCCATCCAA AATTCCGTTA CATTCGTGAT TTGTATCCAA TAATGTTTGG AATCGATGTG
    ATATGCTTCC TCATCATGAC ATTTGGATAC TCGGCATTTG GTGAAGGAGG TTCTGGAAAT
    GTTTTAGATG ATGTGAAGGC TTCTCGTATT CCGGTAACAC TTGTTGTTAT GCTCGTTGGA
    ATGACTCTGG CAATTATCAT TGATCGAGCA CTCTACCTCA GAAAATCAGT TGTCGGAAAG
    CTAATCTATC AAGTCTTAAT GATTGCTTTC CTTCACATTT GGGTATTCCT TGTTCTTCCA
    AATATGACTC GTCGTTCTGC GATATCGAAT CACGTTGCTC AAGCATTGTA CGTCATCAAA
    TCGTGCTATT TCCTTGTGTC TGCTTGGCAG ATCCGAAATG GTTATCCTGA ACTTTGTATT
    GGAAATCTAT TAACACATTC ATACGGAATG ACCAATATGA TTGCATTCAA AGTATTCATG
    AATATTCCAT TCCTCTTCGA GCTTCGTACA GCTATTGATT GGACATGGAC AGATACTTCA
    ATGCCTCTTT TCGATTTCTT CAATATGGAA AATTTCTATG CTCATATATT CAATATCAAG
    TGTGCCCGCC AGTTCGAAGC TGCCTATCCA GCTCCAAGAG GGATTCCCAA AGGAAAGTTG
    GTCAAGTATA TGATGGGATT CCCAATTATC ATTGGAGTCG TTATCTTCAT CTTTTCTCCT
    CTATTGCTCT GGTCGCTACT CAATCAAATC GGAACTATCA GTATGCCAGA AAAAGTTACG
    TTGAGGATTT CGATTGAAGG ATATCCACCA CTTTACGAGA TGGAGGCACA AGGAAGTAAT
    CACGATAACG CTGAACTTGG AATGATAAAA CCTGATCAAT TGGCATCTTT GAATCAAGCA
    TTGACGGATA GTTACACGAC ACGAGACACT AATTCTATTT TAAGATCAAG AATGTCTGTA
    TCATATCTGA AAGGATACAC ATATGAGGAT ATTCTGATTG TGCGATTCCG TCCAGAATCT
    GAAATTTATT GGCCGATTTC ACAGGATTCG AGAAATGCGA TGATCGATAA ATTGAGCCGA
    AACACGTCAG TCAATTTTGA AGTATCTCTG GAATTCACTC GGCCATATGA TCCAAATGAA
    AACGCTGCTT TAAAACATTC GAAATCGTGG CTTGTTCCAA TCTCCCTGGA TATGACGATT
    CGAGCAAAAA TTCAAAGTGC TCTCCGAGGA GATCCAGGTC ATCCGATTCT CATTCCACAG
    TCAATTCCTG CGTTTATTCA AGTTCCAAAT CAAGGAGAAT TGACCCTTCC AACATCAATC
    GGAAATACTA TTATCAATGA CGGAAATCCA CGGATTAATA CGACCGGGAT GGAAAAATCA
    GACGAAGCTC GGGCTTGGTT CGACTCGTTA ACCTTGAATC TCGAACAAGG AAAATCGCAA
    AACGAGAAAA TGTGGATCGC CACATCCGAA CATCCAGGAG ACCAAAATGC GAAACTCTGG
    ATAAAGACTG CAAATACGAC GTATTCGGGA AGGCCGTACC TTCAAGTTGT CGGATTTATT
    GATAGAGCAT TCCCATCATT TTTGGCGAAA GTTTTCAAAG GAGGAGTCAT TGCAGTGTAT
    CTTTCAGTTA TCCTCGTCGT TGGAAGAGGT CTGGTTCGTG GAATCTTCAC CACATCTCCA
    AGCACTGTCA TGTTCACAGA ATTACCGAAT GCGGATCATC TTCTCAAAAT TTGTCTCGAC
    ATCTATTTGG TTCGAGAAGC GAAGGACTTC ATGTTGGAGC AGGATCTCTT CGCCAAATTA
    ATATTCCTGT TCCGATCACC AGCGACACTC ATCGAATGGA CACGAATGAG CAAAAAGAAG
    CAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342184
    Clone ID Related Accession (Same CDS sequence) NM_001268462.3 , NM_001268462.2
    Accession Version NM_001268462.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268462.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACACCCGGAG ATAAGGAATC TGCTGCTTCG AAAGGAATGA TTGCAGTCAT GACATTTGTC
    ATTTTCCATT CGTATTCAAT CGCGCTGACT GCTATGATGA CATGGGCTCT CCTCTATCAT
    TCGATATTCG GTCTCATTCT CCTCATCCTC ACATGTATCT TGTGGATCTT CCGTGATACG
    AGAAAGTCAT CGTTTGCAAT GGCCCCTATT ATTTTGATGT ATATCGAGTT TCTATTGATT
    CTTCAATATT TCCTCAGTAT GGATATTCAT GCAGAAATTG GCGATCCAGC GTGGATGAAC
    TTTGTTGGAA TCGAATGGAC TACTCTTCCA GTACATGCTG TCATCATTCT CTGTGTCCAG
    ACTCTTCTCA CTCTCCCAGT GTTCTTGCTT CTACGTTTGG CTCGCAGGGA GAAGTTTTAC
    GAATCGCTGA GTGATTATGA ACGACAACGA CGAATCAACT CATATGGAAC ATTTGGAGCA
    TCAAAGACTG GAGCTGGGGG TGTTGCAGTG GCTAAGTTTC AGGATCCAAA AAGTCGGAAG
    TTCGCGGCAT TTGTGGAATA TCTGTCGAAT AAAGTGTCTG TCTACTTCAT ATTTGTAGTA
    TCAGTGGTAC TTTTGGTGGT CTCTACGTGT TTTGCTCCGA ATTTCTACAA TATTCTATTC
    TTCGCACTAT GGGCATTGAA CTTGATTTAT CTCAAGTTCT CATTCCGTCT GTATCGTGGA
    TTGGCATATG CATTCTGGCT AACTCTCACG TTCTACACTT CAATTGTTAT TATCGCCCTC
    TACATTTATC AGTTCCCTGG AGTATCTCAA TGGATTATTC GAAATACTAG TTTGTCGCAG
    GAGTGGCTGA ATGCAATTGG ACTTGTCGAT TTTAGAGCTA TCGGTGAATC GGGAGCCCTA
    TTTCTACAAT TACTCGCCCC AATTGCCTTG TTCGTCGTCA CAATGCTCCA ACTCAAGTTT
    TTCCATGGAC CATGGAGTCG AGCAACGTCA CCAAGAAGAG CAGAAAATGA TCCACCTACA
    AGTACGACGG AGGCTGCGGC GGTAGCAAGC ACATCTGGAA CACAAGGAAG AGCACATGCT
    GCAGGCGATA CCCTTGTCAA GAAGCTGCAC AAATTGGCAA ATCAGACAAT CGAACTGTTA
    TGGAGGTTTT TCGAAGTTCA CATTTCGAAA ATTGTCTTTG TCATCATTGC GATTTTTATT
    GCCAACAATA TCAATGCTCT ATACATTCCA CTTGTTATTC TCCTCTCATT GGCTATATGT
    CTACCATCAG CTGCAGATGG GATATTCTCT TTATTTATGT GTGCCTACTT ATTCCTCGTG
    GCACTGAGCA AGATGATCTA CCAACTGGAT ATTGTTCCCG AGTTATCACA AATCGACCGA
    GGAGTTGGAG CAGATAACTG TTCACATGGA AATATTTCAA TGCCTGAATG GTTTGGATTG
    AAGAAGGAAG TTGAAGGAAC TGAACCAATT TATATGCTCT TTGGAGTCAT TGTTTCGATT
    ATTGCATTGG CTTTTCAATC AATTGTTATA TATCGACAAA GACATTATCG AGCTTCACTG
    GGACTTCCTG AATCGATGCG AGCTAAAGTG TTCCCGGACT TCCATCATTC TCATTTTGAT
    AGATCTCTCA AAAATGCCAT CCAATTCTTG ATAGACTATG GTTTCTATAA GTTTGGACTT
    GAAATCACGA TGATCGCCAT TGGAATTGAC ATTTTCAATC GAATGGATGC ATTGGCAGCG
    ATTCAATGTT TCTGGCTTGT CCTGTTTGCG CTCAATAAAA GAGTCTTTGT TCGAAGAATA
    TGGGTATTCT ATGTGATCTA TATGGCGATT CTGTATCCAC TTCAGTTCTT TTCATATGTT
    GGATTACCAC CTGACAGTTG TATTGAGTAT CCATGGTCGT ATTGGATTCC ATCGTATAGT
    GATGATGCGA GATTCAATCT GTCGTATCTT CTGAACTTGT CAATATACGG TGTAAACTGG
    CCATCAGCAT ATCTCATTGG CGATTTCTTC GTACTCCTCT TGGCATCATG CCAATTAGCA
    GTTTTCCGGC GAGAAGGAGA AGATAATGAT TCAATTTACA ATGATGGAAA CTTTGTGATT
    AAGCCAGAAA ATCCACAATA TGACTTTATT GACACCAAAA AGAGTTACGT TGATTATTTC
    AAATCATTCG TTTTCCATTA TGGACACTGG ATTACATTGA TGTCTACACT AGCAGCAGGA
    ATTGCTGGAA CGTCACTTTT TGCTTTGGGA TACATTATAT TCACTCTGAC AATGCTGTGG
    TCTGGTAACA ATTTGTATGT GATGAACTCA ACTCTACGAT CATTTGAACA TACTTTGAAA
    CGATGGAATG CACTTTTGGG ATACACATTG TTCACGATTA CAATGAAAGT TTGCCTTCAA
    ATATTTGGAT GTGTATTCCT GTCGTGGTTC GATCAATCTG GTGGATGGGG AAAGACTCTG
    TGCATCGTTC GTCAACTCTT CAGTATCACA TGTGTTAATA ACGAATGTCA TGTGCTCAAA
    GAGCTGGAGG ATTTCTCGAA GGCGTGTGCT GTTGAGACGA AAGAAGGAAA TATTGGATTT
    GATGTGATTG CGCTATCCTT CCTCGTCTTC CAAATCCGAA TTTTCCATTC TTGGTATTTC
    CAACACTGCA TGGTTGAATA TCGAAGTGAG GTTATTCTGG CCAATAGAGG TGCCGTGCTG
    AAGAATCAAC TGATTGAAAA GGAAATGAAG GAACAAAACG AGCAGCAAAA AGCCAAGTTC
    AATGATATCA GAAGAAGAAC TGAAGCTATT CGTGAAAGAT ATCAAAAACA AATCGAACGA
    GGAGCTGCTG AACGTGACTT CGAGCCGGTT ACTTATGGGC ACGCCAAACG AGCGGGAGAC
    TACTACATGT TCAAATACGA TCCTGAAAAT GATGATCTTG TGGAACCCGT CGACAGTTTT
    GTACCAGAAG TTGATCCCAA GGCAACTGCA TATGATCGAC TTGATCCTGG ACAGATAATG
    TACGCTGCCA CCGCTCACGA CCTCGACCTC GCCAAAACTG TACAGCAAGT CAAGAAAGGC
    GATACTATCA AAGATCCTGA TTCACGTGCT CTTATCGCTG TTTCTGAACC AGAAGCTCGT
    AAGCCAGGAG GCACTGAAGA AACGGATGGT GATGAAGATG AGGATAATAA GGATTCGAAA
    GTGGAAAGTA CTGCTAAATT CATTCAAAAA ATGATCGCTT CTGCCTTGGA CTTGTGTAGT
    GTAACACTAA ACAAACTGTG CAGAGAACAC CGATATGTTG GATTTGTTTT GAGCAAGGAA
    AAACAAAAGT TGAAGTCAGG ACACTCGGAA TCTCTTTCAA ATACATCTAG AAAGCTGACG
    GATATTCGAT CTGCAGTTGA TCTACCAAGT CTTCAACTTG TGCAATCGGC GAATGATGTT
    GAAAAGATGG AGACAGCCGT CAGCGTGGAT TGGCAACAAA AATCATCAGC AACGAGACTA
    TTGAATGCAG TTGTGAATTG TATTGGAGCA CACACTGATA TTCTCTGCTA CTTCTTCGCA
    ATTATGACAC AAGTGATGAC TGGTGGACTT ATTACTCTTC CACTTCCTCT GATGTCACTA
    TTCTGGGGAA ATCTATCGAA TCCTCGTCCA TCCAAGTTCT TCTGGGTCAC AATGATCACC
    TACACTGAGT GTGTCATTGT TATCAAGTTT GTTTGCCAAT TTGCATTCAT GCCTTACAAT
    TCTATCACGT GGCGAACAGA GCATCAAATG GACCCGATGT CATTGGATAA GTTATTCGGA
    GTATCACAGA GAGATAGTTT TGCACTTTGG GACATTGTTT TGCTCTTCTC ACTCTTCTTC
    CATCGGTATA TGCTCAGAAA GTTAGGGTTA TGGAAGGATG CCAATTTGAC GGACACGTTC
    ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA GGATCGGACA CAGGATCACC CAAGAAAATT
    GCTCAAGAGC CAAAGGTTGT TGTAACTCAG TCAGATACCC TTGAAGGTAC AAGTGGAGGA
    GAGATAGTCA TACCATCTGA TCCAAATGCA GTTTCCAATA TGGAAGAATT AGACTGCGAA
    CCTCCGATTC CAGAGAAGCA AAGTGGACCA ATTGGAAGAT TCATTCATCA ATTATTCCAT
    CCAAAATTCC GTTACATTCG TGATTTGTAT CCAATAATGT TTGGAATCGA TGTGATATGC
    TTCCTCATCA TGACATTTGG ATACTCGGCA TTTGGTGAAG GAGGTTCTGG AAATGTTTTA
    GATGATGTGA AGGCTTCTCG TATTCCGGTA ACACTTGTTG TTATGCTCGT TGGAATGACT
    CTGGCAATTA TCATTGATCG AGCACTCTAC CTCAGAAAAT CAGTTGTCGG AAAGCTAATC
    TATCAAGTCT TAATGATTGC TTTCCTTCAC ATTTGGGTAT TCCTTGTTCT TCCAAATATG
    ACTCGTCGTT CTGCGATATC GAATCACGTT GCTCAAGCAT TGTACGTCAT CAAATCGTGC
    TATTTCCTTG TGTCTGCTTG GCAGATCCGA AATGGTTATC CTGAACTTTG TATTGGAAAT
    CTATTAACAC ATTCATACGG AATGACCAAT ATGATTGCAT TCAAAGTATT CATGAATATT
    CCATTCCTCT TCGAGCTTCG TACAGCTATT GATTGGACAT GGACAGATAC TTCAATGCCT
    CTTTTCGATT TCTTCAATAT GGAAAATTTC TATGCTCATA TATTCAATAT CAAGTGTGCC
    CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA AGAGGGATTC CCAAAGGAAA GTTGGTCAAG
    TATATGATGG GATTCCCAAT TATCATTGGA GTCGTTATCT TCATCTTTTC TCCTCTATTG
    CTCTGGTCGC TACTCAATCA AATCGGAACT ATCAGTATGC CAGAAAAAGT TACGTTGAGG
    ATTTCGATTG AAGGATATCC ACCACTTTAC GAGATGGAGG CACAAGGAAG TAATCACGAT
    AACGCTGAAC TTGGAATGAT AAAACCTGAT CAATTGGCAT CTTTGAATCA AGCATTGACG
    GATAGTTACA CGACACGAGA CACTAATTCT ATTTTAAGAT CAAGAATGTC TGTATCATAT
    CTGAAAGGAT ACACATATGA GGATATTCTG ATTGTGCGAT TCCGTCCAGA ATCTGAAATT
    TATTGGCCGA TTTCACAGGA TTCGAGAAAT GCGATGATCG ATAAATTGAG CCGAAACACG
    TCAGTCAATT TTGAAGTATC TCTGGAATTC ACTCGGCCAT ATGATCCAAA TGAAAACGCT
    GCTTTAAAAC ATTCGAAATC GTGGCTTGTT CCAATCTCCC TGGATATGAC GATTCGAGCA
    AAAATTCAAA GTGCTCTCCG AGGAGATCCA GGTCATCCGA TTCTCATTCC ACAGTCAATT
    CCTGCGTTTA TTCAAGTTCC AAATCAAGGA GAATTGACCC TTCCAACATC AATCGGAAAT
    ACTATTATCA ATGACGGAAA TCCACGGATT AATACGACCG GGATGGAAAA ATCAGACGAA
    GCTCGGGCTT GGTTCGACTC GTTAACCTTG AATCTCGAAC AAGGAAAATC GCAAAACGAG
    AAAATGTGGA TCGCCACATC CGAACATCCA GGAGACCAAA ATGCGAAACT CTGGATAAAG
    ACTGCAAATA CGACGTATTC GGGAAGGCCG TACCTTCAAG TTGTCGGATT TATTGATAGA
    GCATTCCCAT CATTTTTGGC GAAAGTTTTC AAAGGAGGAG TCATTGCAGT GTATCTTTCA
    GTTATCCTCG TCGTTGGAAG AGGTCTGGTT CGTGGAATCT TCACCACATC TCCAAGCACT
    GTCATGTTCA CAGAATTACC GAATGCGGAT CATCTTCTCA AAATTTGTCT CGACATCTAT
    TTGGTTCGAG AAGCGAAGGA CTTCATGTTG GAGCAGGATC TCTTCGCCAA ATTAATATTC
    CTGTTCCGAT CACCAGCGAC ACTCATCGAA TGGACACGAA TGAGCAAAAA GAAGCAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255391.2
    CDS57..7379
    Translation

    Target ORF information:

    RefSeq Version NM_001268462.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268462.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACACCCGGAG ATAAGGAATC TGCTGCTTCG AAAGGAATGA TTGCAGTCAT GACATTTGTC
    ATTTTCCATT CGTATTCAAT CGCGCTGACT GCTATGATGA CATGGGCTCT CCTCTATCAT
    TCGATATTCG GTCTCATTCT CCTCATCCTC ACATGTATCT TGTGGATCTT CCGTGATACG
    AGAAAGTCAT CGTTTGCAAT GGCCCCTATT ATTTTGATGT ATATCGAGTT TCTATTGATT
    CTTCAATATT TCCTCAGTAT GGATATTCAT GCAGAAATTG GCGATCCAGC GTGGATGAAC
    TTTGTTGGAA TCGAATGGAC TACTCTTCCA GTACATGCTG TCATCATTCT CTGTGTCCAG
    ACTCTTCTCA CTCTCCCAGT GTTCTTGCTT CTACGTTTGG CTCGCAGGGA GAAGTTTTAC
    GAATCGCTGA GTGATTATGA ACGACAACGA CGAATCAACT CATATGGAAC ATTTGGAGCA
    TCAAAGACTG GAGCTGGGGG TGTTGCAGTG GCTAAGTTTC AGGATCCAAA AAGTCGGAAG
    TTCGCGGCAT TTGTGGAATA TCTGTCGAAT AAAGTGTCTG TCTACTTCAT ATTTGTAGTA
    TCAGTGGTAC TTTTGGTGGT CTCTACGTGT TTTGCTCCGA ATTTCTACAA TATTCTATTC
    TTCGCACTAT GGGCATTGAA CTTGATTTAT CTCAAGTTCT CATTCCGTCT GTATCGTGGA
    TTGGCATATG CATTCTGGCT AACTCTCACG TTCTACACTT CAATTGTTAT TATCGCCCTC
    TACATTTATC AGTTCCCTGG AGTATCTCAA TGGATTATTC GAAATACTAG TTTGTCGCAG
    GAGTGGCTGA ATGCAATTGG ACTTGTCGAT TTTAGAGCTA TCGGTGAATC GGGAGCCCTA
    TTTCTACAAT TACTCGCCCC AATTGCCTTG TTCGTCGTCA CAATGCTCCA ACTCAAGTTT
    TTCCATGGAC CATGGAGTCG AGCAACGTCA CCAAGAAGAG CAGAAAATGA TCCACCTACA
    AGTACGACGG AGGCTGCGGC GGTAGCAAGC ACATCTGGAA CACAAGGAAG AGCACATGCT
    GCAGGCGATA CCCTTGTCAA GAAGCTGCAC AAATTGGCAA ATCAGACAAT CGAACTGTTA
    TGGAGGTTTT TCGAAGTTCA CATTTCGAAA ATTGTCTTTG TCATCATTGC GATTTTTATT
    GCCAACAATA TCAATGCTCT ATACATTCCA CTTGTTATTC TCCTCTCATT GGCTATATGT
    CTACCATCAG CTGCAGATGG GATATTCTCT TTATTTATGT GTGCCTACTT ATTCCTCGTG
    GCACTGAGCA AGATGATCTA CCAACTGGAT ATTGTTCCCG AGTTATCACA AATCGACCGA
    GGAGTTGGAG CAGATAACTG TTCACATGGA AATATTTCAA TGCCTGAATG GTTTGGATTG
    AAGAAGGAAG TTGAAGGAAC TGAACCAATT TATATGCTCT TTGGAGTCAT TGTTTCGATT
    ATTGCATTGG CTTTTCAATC AATTGTTATA TATCGACAAA GACATTATCG AGCTTCACTG
    GGACTTCCTG AATCGATGCG AGCTAAAGTG TTCCCGGACT TCCATCATTC TCATTTTGAT
    AGATCTCTCA AAAATGCCAT CCAATTCTTG ATAGACTATG GTTTCTATAA GTTTGGACTT
    GAAATCACGA TGATCGCCAT TGGAATTGAC ATTTTCAATC GAATGGATGC ATTGGCAGCG
    ATTCAATGTT TCTGGCTTGT CCTGTTTGCG CTCAATAAAA GAGTCTTTGT TCGAAGAATA
    TGGGTATTCT ATGTGATCTA TATGGCGATT CTGTATCCAC TTCAGTTCTT TTCATATGTT
    GGATTACCAC CTGACAGTTG TATTGAGTAT CCATGGTCGT ATTGGATTCC ATCGTATAGT
    GATGATGCGA GATTCAATCT GTCGTATCTT CTGAACTTGT CAATATACGG TGTAAACTGG
    CCATCAGCAT ATCTCATTGG CGATTTCTTC GTACTCCTCT TGGCATCATG CCAATTAGCA
    GTTTTCCGGC GAGAAGGAGA AGATAATGAT TCAATTTACA ATGATGGAAA CTTTGTGATT
    AAGCCAGAAA ATCCACAATA TGACTTTATT GACACCAAAA AGAGTTACGT TGATTATTTC
    AAATCATTCG TTTTCCATTA TGGACACTGG ATTACATTGA TGTCTACACT AGCAGCAGGA
    ATTGCTGGAA CGTCACTTTT TGCTTTGGGA TACATTATAT TCACTCTGAC AATGCTGTGG
    TCTGGTAACA ATTTGTATGT GATGAACTCA ACTCTACGAT CATTTGAACA TACTTTGAAA
    CGATGGAATG CACTTTTGGG ATACACATTG TTCACGATTA CAATGAAAGT TTGCCTTCAA
    ATATTTGGAT GTGTATTCCT GTCGTGGTTC GATCAATCTG GTGGATGGGG AAAGACTCTG
    TGCATCGTTC GTCAACTCTT CAGTATCACA TGTGTTAATA ACGAATGTCA TGTGCTCAAA
    GAGCTGGAGG ATTTCTCGAA GGCGTGTGCT GTTGAGACGA AAGAAGGAAA TATTGGATTT
    GATGTGATTG CGCTATCCTT CCTCGTCTTC CAAATCCGAA TTTTCCATTC TTGGTATTTC
    CAACACTGCA TGGTTGAATA TCGAAGTGAG GTTATTCTGG CCAATAGAGG TGCCGTGCTG
    AAGAATCAAC TGATTGAAAA GGAAATGAAG GAACAAAACG AGCAGCAAAA AGCCAAGTTC
    AATGATATCA GAAGAAGAAC TGAAGCTATT CGTGAAAGAT ATCAAAAACA AATCGAACGA
    GGAGCTGCTG AACGTGACTT CGAGCCGGTT ACTTATGGGC ACGCCAAACG AGCGGGAGAC
    TACTACATGT TCAAATACGA TCCTGAAAAT GATGATCTTG TGGAACCCGT CGACAGTTTT
    GTACCAGAAG TTGATCCCAA GGCAACTGCA TATGATCGAC TTGATCCTGG ACAGATAATG
    TACGCTGCCA CCGCTCACGA CCTCGACCTC GCCAAAACTG TACAGCAAGT CAAGAAAGGC
    GATACTATCA AAGATCCTGA TTCACGTGCT CTTATCGCTG TTTCTGAACC AGAAGCTCGT
    AAGCCAGGAG GCACTGAAGA AACGGATGGT GATGAAGATG AGGATAATAA GGATTCGAAA
    GTGGAAAGTA CTGCTAAATT CATTCAAAAA ATGATCGCTT CTGCCTTGGA CTTGTGTAGT
    GTAACACTAA ACAAACTGTG CAGAGAACAC CGATATGTTG GATTTGTTTT GAGCAAGGAA
    AAACAAAAGT TGAAGTCAGG ACACTCGGAA TCTCTTTCAA ATACATCTAG AAAGCTGACG
    GATATTCGAT CTGCAGTTGA TCTACCAAGT CTTCAACTTG TGCAATCGGC GAATGATGTT
    GAAAAGATGG AGACAGCCGT CAGCGTGGAT TGGCAACAAA AATCATCAGC AACGAGACTA
    TTGAATGCAG TTGTGAATTG TATTGGAGCA CACACTGATA TTCTCTGCTA CTTCTTCGCA
    ATTATGACAC AAGTGATGAC TGGTGGACTT ATTACTCTTC CACTTCCTCT GATGTCACTA
    TTCTGGGGAA ATCTATCGAA TCCTCGTCCA TCCAAGTTCT TCTGGGTCAC AATGATCACC
    TACACTGAGT GTGTCATTGT TATCAAGTTT GTTTGCCAAT TTGCATTCAT GCCTTACAAT
    TCTATCACGT GGCGAACAGA GCATCAAATG GACCCGATGT CATTGGATAA GTTATTCGGA
    GTATCACAGA GAGATAGTTT TGCACTTTGG GACATTGTTT TGCTCTTCTC ACTCTTCTTC
    CATCGGTATA TGCTCAGAAA GTTAGGGTTA TGGAAGGATG CCAATTTGAC GGACACGTTC
    ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA GGATCGGACA CAGGATCACC CAAGAAAATT
    GCTCAAGAGC CAAAGGTTGT TGTAACTCAG TCAGATACCC TTGAAGGTAC AAGTGGAGGA
    GAGATAGTCA TACCATCTGA TCCAAATGCA GTTTCCAATA TGGAAGAATT AGACTGCGAA
    CCTCCGATTC CAGAGAAGCA AAGTGGACCA ATTGGAAGAT TCATTCATCA ATTATTCCAT
    CCAAAATTCC GTTACATTCG TGATTTGTAT CCAATAATGT TTGGAATCGA TGTGATATGC
    TTCCTCATCA TGACATTTGG ATACTCGGCA TTTGGTGAAG GAGGTTCTGG AAATGTTTTA
    GATGATGTGA AGGCTTCTCG TATTCCGGTA ACACTTGTTG TTATGCTCGT TGGAATGACT
    CTGGCAATTA TCATTGATCG AGCACTCTAC CTCAGAAAAT CAGTTGTCGG AAAGCTAATC
    TATCAAGTCT TAATGATTGC TTTCCTTCAC ATTTGGGTAT TCCTTGTTCT TCCAAATATG
    ACTCGTCGTT CTGCGATATC GAATCACGTT GCTCAAGCAT TGTACGTCAT CAAATCGTGC
    TATTTCCTTG TGTCTGCTTG GCAGATCCGA AATGGTTATC CTGAACTTTG TATTGGAAAT
    CTATTAACAC ATTCATACGG AATGACCAAT ATGATTGCAT TCAAAGTATT CATGAATATT
    CCATTCCTCT TCGAGCTTCG TACAGCTATT GATTGGACAT GGACAGATAC TTCAATGCCT
    CTTTTCGATT TCTTCAATAT GGAAAATTTC TATGCTCATA TATTCAATAT CAAGTGTGCC
    CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA AGAGGGATTC CCAAAGGAAA GTTGGTCAAG
    TATATGATGG GATTCCCAAT TATCATTGGA GTCGTTATCT TCATCTTTTC TCCTCTATTG
    CTCTGGTCGC TACTCAATCA AATCGGAACT ATCAGTATGC CAGAAAAAGT TACGTTGAGG
    ATTTCGATTG AAGGATATCC ACCACTTTAC GAGATGGAGG CACAAGGAAG TAATCACGAT
    AACGCTGAAC TTGGAATGAT AAAACCTGAT CAATTGGCAT CTTTGAATCA AGCATTGACG
    GATAGTTACA CGACACGAGA CACTAATTCT ATTTTAAGAT CAAGAATGTC TGTATCATAT
    CTGAAAGGAT ACACATATGA GGATATTCTG ATTGTGCGAT TCCGTCCAGA ATCTGAAATT
    TATTGGCCGA TTTCACAGGA TTCGAGAAAT GCGATGATCG ATAAATTGAG CCGAAACACG
    TCAGTCAATT TTGAAGTATC TCTGGAATTC ACTCGGCCAT ATGATCCAAA TGAAAACGCT
    GCTTTAAAAC ATTCGAAATC GTGGCTTGTT CCAATCTCCC TGGATATGAC GATTCGAGCA
    AAAATTCAAA GTGCTCTCCG AGGAGATCCA GGTCATCCGA TTCTCATTCC ACAGTCAATT
    CCTGCGTTTA TTCAAGTTCC AAATCAAGGA GAATTGACCC TTCCAACATC AATCGGAAAT
    ACTATTATCA ATGACGGAAA TCCACGGATT AATACGACCG GGATGGAAAA ATCAGACGAA
    GCTCGGGCTT GGTTCGACTC GTTAACCTTG AATCTCGAAC AAGGAAAATC GCAAAACGAG
    AAAATGTGGA TCGCCACATC CGAACATCCA GGAGACCAAA ATGCGAAACT CTGGATAAAG
    ACTGCAAATA CGACGTATTC GGGAAGGCCG TACCTTCAAG TTGTCGGATT TATTGATAGA
    GCATTCCCAT CATTTTTGGC GAAAGTTTTC AAAGGAGGAG TCATTGCAGT GTATCTTTCA
    GTTATCCTCG TCGTTGGAAG AGGTCTGGTT CGTGGAATCT TCACCACATC TCCAAGCACT
    GTCATGTTCA CAGAATTACC GAATGCGGAT CATCTTCTCA AAATTTGTCT CGACATCTAT
    TTGGTTCGAG AAGCGAAGGA CTTCATGTTG GAGCAGGATC TCTTCGCCAA ATTAATATTC
    CTGTTCCGAT CACCAGCGAC ACTCATCGAA TGGACACGAA TGAGCAAAAA GAAGCAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342184
    Clone ID Related Accession (Same CDS sequence) NM_001268462.3 , NM_001268462.2
    Accession Version NM_001268462.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Apr 20, 2015 this sequence version replaced NM_001268462.1. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACACCCGGAG ATAAGGAATC TGCTGCTTCG AAAGGAATGA TTGCAGTCAT GACATTTGTC
    ATTTTCCATT CGTATTCAAT CGCGCTGACT GCTATGATGA CATGGGCTCT CCTCTATCAT
    TCGATATTCG GTCTCATTCT CCTCATCCTC ACATGTATCT TGTGGATCTT CCGTGATACG
    AGAAAGTCAT CGTTTGCAAT GGCCCCTATT ATTTTGATGT ATATCGAGTT TCTATTGATT
    CTTCAATATT TCCTCAGTAT GGATATTCAT GCAGAAATTG GCGATCCAGC GTGGATGAAC
    TTTGTTGGAA TCGAATGGAC TACTCTTCCA GTACATGCTG TCATCATTCT CTGTGTCCAG
    ACTCTTCTCA CTCTCCCAGT GTTCTTGCTT CTACGTTTGG CTCGCAGGGA GAAGTTTTAC
    GAATCGCTGA GTGATTATGA ACGACAACGA CGAATCAACT CATATGGAAC ATTTGGAGCA
    TCAAAGACTG GAGCTGGGGG TGTTGCAGTG GCTAAGTTTC AGGATCCAAA AAGTCGGAAG
    TTCGCGGCAT TTGTGGAATA TCTGTCGAAT AAAGTGTCTG TCTACTTCAT ATTTGTAGTA
    TCAGTGGTAC TTTTGGTGGT CTCTACGTGT TTTGCTCCGA ATTTCTACAA TATTCTATTC
    TTCGCACTAT GGGCATTGAA CTTGATTTAT CTCAAGTTCT CATTCCGTCT GTATCGTGGA
    TTGGCATATG CATTCTGGCT AACTCTCACG TTCTACACTT CAATTGTTAT TATCGCCCTC
    TACATTTATC AGTTCCCTGG AGTATCTCAA TGGATTATTC GAAATACTAG TTTGTCGCAG
    GAGTGGCTGA ATGCAATTGG ACTTGTCGAT TTTAGAGCTA TCGGTGAATC GGGAGCCCTA
    TTTCTACAAT TACTCGCCCC AATTGCCTTG TTCGTCGTCA CAATGCTCCA ACTCAAGTTT
    TTCCATGGAC CATGGAGTCG AGCAACGTCA CCAAGAAGAG CAGAAAATGA TCCACCTACA
    AGTACGACGG AGGCTGCGGC GGTAGCAAGC ACATCTGGAA CACAAGGAAG AGCACATGCT
    GCAGGCGATA CCCTTGTCAA GAAGCTGCAC AAATTGGCAA ATCAGACAAT CGAACTGTTA
    TGGAGGTTTT TCGAAGTTCA CATTTCGAAA ATTGTCTTTG TCATCATTGC GATTTTTATT
    GCCAACAATA TCAATGCTCT ATACATTCCA CTTGTTATTC TCCTCTCATT GGCTATATGT
    CTACCATCAG CTGCAGATGG GATATTCTCT TTATTTATGT GTGCCTACTT ATTCCTCGTG
    GCACTGAGCA AGATGATCTA CCAACTGGAT ATTGTTCCCG AGTTATCACA AATCGACCGA
    GGAGTTGGAG CAGATAACTG TTCACATGGA AATATTTCAA TGCCTGAATG GTTTGGATTG
    AAGAAGGAAG TTGAAGGAAC TGAACCAATT TATATGCTCT TTGGAGTCAT TGTTTCGATT
    ATTGCATTGG CTTTTCAATC AATTGTTATA TATCGACAAA GACATTATCG AGCTTCACTG
    GGACTTCCTG AATCGATGCG AGCTAAAGTG TTCCCGGACT TCCATCATTC TCATTTTGAT
    AGATCTCTCA AAAATGCCAT CCAATTCTTG ATAGACTATG GTTTCTATAA GTTTGGACTT
    GAAATCACGA TGATCGCCAT TGGAATTGAC ATTTTCAATC GAATGGATGC ATTGGCAGCG
    ATTCAATGTT TCTGGCTTGT CCTGTTTGCG CTCAATAAAA GAGTCTTTGT TCGAAGAATA
    TGGGTATTCT ATGTGATCTA TATGGCGATT CTGTATCCAC TTCAGTTCTT TTCATATGTT
    GGATTACCAC CTGACAGTTG TATTGAGTAT CCATGGTCGT ATTGGATTCC ATCGTATAGT
    GATGATGCGA GATTCAATCT GTCGTATCTT CTGAACTTGT CAATATACGG TGTAAACTGG
    CCATCAGCAT ATCTCATTGG CGATTTCTTC GTACTCCTCT TGGCATCATG CCAATTAGCA
    GTTTTCCGGC GAGAAGGAGA AGATAATGAT TCAATTTACA ATGATGGAAA CTTTGTGATT
    AAGCCAGAAA ATCCACAATA TGACTTTATT GACACCAAAA AGAGTTACGT TGATTATTTC
    AAATCATTCG TTTTCCATTA TGGACACTGG ATTACATTGA TGTCTACACT AGCAGCAGGA
    ATTGCTGGAA CGTCACTTTT TGCTTTGGGA TACATTATAT TCACTCTGAC AATGCTGTGG
    TCTGGTAACA ATTTGTATGT GATGAACTCA ACTCTACGAT CATTTGAACA TACTTTGAAA
    CGATGGAATG CACTTTTGGG ATACACATTG TTCACGATTA CAATGAAAGT TTGCCTTCAA
    ATATTTGGAT GTGTATTCCT GTCGTGGTTC GATCAATCTG GTGGATGGGG AAAGACTCTG
    TGCATCGTTC GTCAACTCTT CAGTATCACA TGTGTTAATA ACGAATGTCA TGTGCTCAAA
    GAGCTGGAGG ATTTCTCGAA GGCGTGTGCT GTTGAGACGA AAGAAGGAAA TATTGGATTT
    GATGTGATTG CGCTATCCTT CCTCGTCTTC CAAATCCGAA TTTTCCATTC TTGGTATTTC
    CAACACTGCA TGGTTGAATA TCGAAGTGAG GTTATTCTGG CCAATAGAGG TGCCGTGCTG
    AAGAATCAAC TGATTGAAAA GGAAATGAAG GAACAAAACG AGCAGCAAAA AGCCAAGTTC
    AATGATATCA GAAGAAGAAC TGAAGCTATT CGTGAAAGAT ATCAAAAACA AATCGAACGA
    GGAGCTGCTG AACGTGACTT CGAGCCGGTT ACTTATGGGC ACGCCAAACG AGCGGGAGAC
    TACTACATGT TCAAATACGA TCCTGAAAAT GATGATCTTG TGGAACCCGT CGACAGTTTT
    GTACCAGAAG TTGATCCCAA GGCAACTGCA TATGATCGAC TTGATCCTGG ACAGATAATG
    TACGCTGCCA CCGCTCACGA CCTCGACCTC GCCAAAACTG TACAGCAAGT CAAGAAAGGC
    GATACTATCA AAGATCCTGA TTCACGTGCT CTTATCGCTG TTTCTGAACC AGAAGCTCGT
    AAGCCAGGAG GCACTGAAGA AACGGATGGT GATGAAGATG AGGATAATAA GGATTCGAAA
    GTGGAAAGTA CTGCTAAATT CATTCAAAAA ATGATCGCTT CTGCCTTGGA CTTGTGTAGT
    GTAACACTAA ACAAACTGTG CAGAGAACAC CGATATGTTG GATTTGTTTT GAGCAAGGAA
    AAACAAAAGT TGAAGTCAGG ACACTCGGAA TCTCTTTCAA ATACATCTAG AAAGCTGACG
    GATATTCGAT CTGCAGTTGA TCTACCAAGT CTTCAACTTG TGCAATCGGC GAATGATGTT
    GAAAAGATGG AGACAGCCGT CAGCGTGGAT TGGCAACAAA AATCATCAGC AACGAGACTA
    TTGAATGCAG TTGTGAATTG TATTGGAGCA CACACTGATA TTCTCTGCTA CTTCTTCGCA
    ATTATGACAC AAGTGATGAC TGGTGGACTT ATTACTCTTC CACTTCCTCT GATGTCACTA
    TTCTGGGGAA ATCTATCGAA TCCTCGTCCA TCCAAGTTCT TCTGGGTCAC AATGATCACC
    TACACTGAGT GTGTCATTGT TATCAAGTTT GTTTGCCAAT TTGCATTCAT GCCTTACAAT
    TCTATCACGT GGCGAACAGA GCATCAAATG GACCCGATGT CATTGGATAA GTTATTCGGA
    GTATCACAGA GAGATAGTTT TGCACTTTGG GACATTGTTT TGCTCTTCTC ACTCTTCTTC
    CATCGGTATA TGCTCAGAAA GTTAGGGTTA TGGAAGGATG CCAATTTGAC GGACACGTTC
    ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA GGATCGGACA CAGGATCACC CAAGAAAATT
    GCTCAAGAGC CAAAGGTTGT TGTAACTCAG TCAGATACCC TTGAAGGTAC AAGTGGAGGA
    GAGATAGTCA TACCATCTGA TCCAAATGCA GTTTCCAATA TGGAAGAATT AGACTGCGAA
    CCTCCGATTC CAGAGAAGCA AAGTGGACCA ATTGGAAGAT TCATTCATCA ATTATTCCAT
    CCAAAATTCC GTTACATTCG TGATTTGTAT CCAATAATGT TTGGAATCGA TGTGATATGC
    TTCCTCATCA TGACATTTGG ATACTCGGCA TTTGGTGAAG GAGGTTCTGG AAATGTTTTA
    GATGATGTGA AGGCTTCTCG TATTCCGGTA ACACTTGTTG TTATGCTCGT TGGAATGACT
    CTGGCAATTA TCATTGATCG AGCACTCTAC CTCAGAAAAT CAGTTGTCGG AAAGCTAATC
    TATCAAGTCT TAATGATTGC TTTCCTTCAC ATTTGGGTAT TCCTTGTTCT TCCAAATATG
    ACTCGTCGTT CTGCGATATC GAATCACGTT GCTCAAGCAT TGTACGTCAT CAAATCGTGC
    TATTTCCTTG TGTCTGCTTG GCAGATCCGA AATGGTTATC CTGAACTTTG TATTGGAAAT
    CTATTAACAC ATTCATACGG AATGACCAAT ATGATTGCAT TCAAAGTATT CATGAATATT
    CCATTCCTCT TCGAGCTTCG TACAGCTATT GATTGGACAT GGACAGATAC TTCAATGCCT
    CTTTTCGATT TCTTCAATAT GGAAAATTTC TATGCTCATA TATTCAATAT CAAGTGTGCC
    CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA AGAGGGATTC CCAAAGGAAA GTTGGTCAAG
    TATATGATGG GATTCCCAAT TATCATTGGA GTCGTTATCT TCATCTTTTC TCCTCTATTG
    CTCTGGTCGC TACTCAATCA AATCGGAACT ATCAGTATGC CAGAAAAAGT TACGTTGAGG
    ATTTCGATTG AAGGATATCC ACCACTTTAC GAGATGGAGG CACAAGGAAG TAATCACGAT
    AACGCTGAAC TTGGAATGAT AAAACCTGAT CAATTGGCAT CTTTGAATCA AGCATTGACG
    GATAGTTACA CGACACGAGA CACTAATTCT ATTTTAAGAT CAAGAATGTC TGTATCATAT
    CTGAAAGGAT ACACATATGA GGATATTCTG ATTGTGCGAT TCCGTCCAGA ATCTGAAATT
    TATTGGCCGA TTTCACAGGA TTCGAGAAAT GCGATGATCG ATAAATTGAG CCGAAACACG
    TCAGTCAATT TTGAAGTATC TCTGGAATTC ACTCGGCCAT ATGATCCAAA TGAAAACGCT
    GCTTTAAAAC ATTCGAAATC GTGGCTTGTT CCAATCTCCC TGGATATGAC GATTCGAGCA
    AAAATTCAAA GTGCTCTCCG AGGAGATCCA GGTCATCCGA TTCTCATTCC ACAGTCAATT
    CCTGCGTTTA TTCAAGTTCC AAATCAAGGA GAATTGACCC TTCCAACATC AATCGGAAAT
    ACTATTATCA ATGACGGAAA TCCACGGATT AATACGACCG GGATGGAAAA ATCAGACGAA
    GCTCGGGCTT GGTTCGACTC GTTAACCTTG AATCTCGAAC AAGGAAAATC GCAAAACGAG
    AAAATGTGGA TCGCCACATC CGAACATCCA GGAGACCAAA ATGCGAAACT CTGGATAAAG
    ACTGCAAATA CGACGTATTC GGGAAGGCCG TACCTTCAAG TTGTCGGATT TATTGATAGA
    GCATTCCCAT CATTTTTGGC GAAAGTTTTC AAAGGAGGAG TCATTGCAGT GTATCTTTCA
    GTTATCCTCG TCGTTGGAAG AGGTCTGGTT CGTGGAATCT TCACCACATC TCCAAGCACT
    GTCATGTTCA CAGAATTACC GAATGCGGAT CATCTTCTCA AAATTTGTCT CGACATCTAT
    TTGGTTCGAG AAGCGAAGGA CTTCATGTTG GAGCAGGATC TCTTCGCCAA ATTAATATTC
    CTGTTCCGAT CACCAGCGAC ACTCATCGAA TGGACACGAA TGAGCAAAAA GAAGCAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255391.2
    CDS1..7323
    Translation

    Target ORF information:

    RefSeq Version NM_001268462.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268462.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACACCCGGAG ATAAGGAATC TGCTGCTTCG AAAGGAATGA TTGCAGTCAT GACATTTGTC
    ATTTTCCATT CGTATTCAAT CGCGCTGACT GCTATGATGA CATGGGCTCT CCTCTATCAT
    TCGATATTCG GTCTCATTCT CCTCATCCTC ACATGTATCT TGTGGATCTT CCGTGATACG
    AGAAAGTCAT CGTTTGCAAT GGCCCCTATT ATTTTGATGT ATATCGAGTT TCTATTGATT
    CTTCAATATT TCCTCAGTAT GGATATTCAT GCAGAAATTG GCGATCCAGC GTGGATGAAC
    TTTGTTGGAA TCGAATGGAC TACTCTTCCA GTACATGCTG TCATCATTCT CTGTGTCCAG
    ACTCTTCTCA CTCTCCCAGT GTTCTTGCTT CTACGTTTGG CTCGCAGGGA GAAGTTTTAC
    GAATCGCTGA GTGATTATGA ACGACAACGA CGAATCAACT CATATGGAAC ATTTGGAGCA
    TCAAAGACTG GAGCTGGGGG TGTTGCAGTG GCTAAGTTTC AGGATCCAAA AAGTCGGAAG
    TTCGCGGCAT TTGTGGAATA TCTGTCGAAT AAAGTGTCTG TCTACTTCAT ATTTGTAGTA
    TCAGTGGTAC TTTTGGTGGT CTCTACGTGT TTTGCTCCGA ATTTCTACAA TATTCTATTC
    TTCGCACTAT GGGCATTGAA CTTGATTTAT CTCAAGTTCT CATTCCGTCT GTATCGTGGA
    TTGGCATATG CATTCTGGCT AACTCTCACG TTCTACACTT CAATTGTTAT TATCGCCCTC
    TACATTTATC AGTTCCCTGG AGTATCTCAA TGGATTATTC GAAATACTAG TTTGTCGCAG
    GAGTGGCTGA ATGCAATTGG ACTTGTCGAT TTTAGAGCTA TCGGTGAATC GGGAGCCCTA
    TTTCTACAAT TACTCGCCCC AATTGCCTTG TTCGTCGTCA CAATGCTCCA ACTCAAGTTT
    TTCCATGGAC CATGGAGTCG AGCAACGTCA CCAAGAAGAG CAGAAAATGA TCCACCTACA
    AGTACGACGG AGGCTGCGGC GGTAGCAAGC ACATCTGGAA CACAAGGAAG AGCACATGCT
    GCAGGCGATA CCCTTGTCAA GAAGCTGCAC AAATTGGCAA ATCAGACAAT CGAACTGTTA
    TGGAGGTTTT TCGAAGTTCA CATTTCGAAA ATTGTCTTTG TCATCATTGC GATTTTTATT
    GCCAACAATA TCAATGCTCT ATACATTCCA CTTGTTATTC TCCTCTCATT GGCTATATGT
    CTACCATCAG CTGCAGATGG GATATTCTCT TTATTTATGT GTGCCTACTT ATTCCTCGTG
    GCACTGAGCA AGATGATCTA CCAACTGGAT ATTGTTCCCG AGTTATCACA AATCGACCGA
    GGAGTTGGAG CAGATAACTG TTCACATGGA AATATTTCAA TGCCTGAATG GTTTGGATTG
    AAGAAGGAAG TTGAAGGAAC TGAACCAATT TATATGCTCT TTGGAGTCAT TGTTTCGATT
    ATTGCATTGG CTTTTCAATC AATTGTTATA TATCGACAAA GACATTATCG AGCTTCACTG
    GGACTTCCTG AATCGATGCG AGCTAAAGTG TTCCCGGACT TCCATCATTC TCATTTTGAT
    AGATCTCTCA AAAATGCCAT CCAATTCTTG ATAGACTATG GTTTCTATAA GTTTGGACTT
    GAAATCACGA TGATCGCCAT TGGAATTGAC ATTTTCAATC GAATGGATGC ATTGGCAGCG
    ATTCAATGTT TCTGGCTTGT CCTGTTTGCG CTCAATAAAA GAGTCTTTGT TCGAAGAATA
    TGGGTATTCT ATGTGATCTA TATGGCGATT CTGTATCCAC TTCAGTTCTT TTCATATGTT
    GGATTACCAC CTGACAGTTG TATTGAGTAT CCATGGTCGT ATTGGATTCC ATCGTATAGT
    GATGATGCGA GATTCAATCT GTCGTATCTT CTGAACTTGT CAATATACGG TGTAAACTGG
    CCATCAGCAT ATCTCATTGG CGATTTCTTC GTACTCCTCT TGGCATCATG CCAATTAGCA
    GTTTTCCGGC GAGAAGGAGA AGATAATGAT TCAATTTACA ATGATGGAAA CTTTGTGATT
    AAGCCAGAAA ATCCACAATA TGACTTTATT GACACCAAAA AGAGTTACGT TGATTATTTC
    AAATCATTCG TTTTCCATTA TGGACACTGG ATTACATTGA TGTCTACACT AGCAGCAGGA
    ATTGCTGGAA CGTCACTTTT TGCTTTGGGA TACATTATAT TCACTCTGAC AATGCTGTGG
    TCTGGTAACA ATTTGTATGT GATGAACTCA ACTCTACGAT CATTTGAACA TACTTTGAAA
    CGATGGAATG CACTTTTGGG ATACACATTG TTCACGATTA CAATGAAAGT TTGCCTTCAA
    ATATTTGGAT GTGTATTCCT GTCGTGGTTC GATCAATCTG GTGGATGGGG AAAGACTCTG
    TGCATCGTTC GTCAACTCTT CAGTATCACA TGTGTTAATA ACGAATGTCA TGTGCTCAAA
    GAGCTGGAGG ATTTCTCGAA GGCGTGTGCT GTTGAGACGA AAGAAGGAAA TATTGGATTT
    GATGTGATTG CGCTATCCTT CCTCGTCTTC CAAATCCGAA TTTTCCATTC TTGGTATTTC
    CAACACTGCA TGGTTGAATA TCGAAGTGAG GTTATTCTGG CCAATAGAGG TGCCGTGCTG
    AAGAATCAAC TGATTGAAAA GGAAATGAAG GAACAAAACG AGCAGCAAAA AGCCAAGTTC
    AATGATATCA GAAGAAGAAC TGAAGCTATT CGTGAAAGAT ATCAAAAACA AATCGAACGA
    GGAGCTGCTG AACGTGACTT CGAGCCGGTT ACTTATGGGC ACGCCAAACG AGCGGGAGAC
    TACTACATGT TCAAATACGA TCCTGAAAAT GATGATCTTG TGGAACCCGT CGACAGTTTT
    GTACCAGAAG TTGATCCCAA GGCAACTGCA TATGATCGAC TTGATCCTGG ACAGATAATG
    TACGCTGCCA CCGCTCACGA CCTCGACCTC GCCAAAACTG TACAGCAAGT CAAGAAAGGC
    GATACTATCA AAGATCCTGA TTCACGTGCT CTTATCGCTG TTTCTGAACC AGAAGCTCGT
    AAGCCAGGAG GCACTGAAGA AACGGATGGT GATGAAGATG AGGATAATAA GGATTCGAAA
    GTGGAAAGTA CTGCTAAATT CATTCAAAAA ATGATCGCTT CTGCCTTGGA CTTGTGTAGT
    GTAACACTAA ACAAACTGTG CAGAGAACAC CGATATGTTG GATTTGTTTT GAGCAAGGAA
    AAACAAAAGT TGAAGTCAGG ACACTCGGAA TCTCTTTCAA ATACATCTAG AAAGCTGACG
    GATATTCGAT CTGCAGTTGA TCTACCAAGT CTTCAACTTG TGCAATCGGC GAATGATGTT
    GAAAAGATGG AGACAGCCGT CAGCGTGGAT TGGCAACAAA AATCATCAGC AACGAGACTA
    TTGAATGCAG TTGTGAATTG TATTGGAGCA CACACTGATA TTCTCTGCTA CTTCTTCGCA
    ATTATGACAC AAGTGATGAC TGGTGGACTT ATTACTCTTC CACTTCCTCT GATGTCACTA
    TTCTGGGGAA ATCTATCGAA TCCTCGTCCA TCCAAGTTCT TCTGGGTCAC AATGATCACC
    TACACTGAGT GTGTCATTGT TATCAAGTTT GTTTGCCAAT TTGCATTCAT GCCTTACAAT
    TCTATCACGT GGCGAACAGA GCATCAAATG GACCCGATGT CATTGGATAA GTTATTCGGA
    GTATCACAGA GAGATAGTTT TGCACTTTGG GACATTGTTT TGCTCTTCTC ACTCTTCTTC
    CATCGGTATA TGCTCAGAAA GTTAGGGTTA TGGAAGGATG CCAATTTGAC GGACACGTTC
    ACTTTGAAGG AAGAGCCTCG ATCGGCGTCA GGATCGGACA CAGGATCACC CAAGAAAATT
    GCTCAAGAGC CAAAGGTTGT TGTAACTCAG TCAGATACCC TTGAAGGTAC AAGTGGAGGA
    GAGATAGTCA TACCATCTGA TCCAAATGCA GTTTCCAATA TGGAAGAATT AGACTGCGAA
    CCTCCGATTC CAGAGAAGCA AAGTGGACCA ATTGGAAGAT TCATTCATCA ATTATTCCAT
    CCAAAATTCC GTTACATTCG TGATTTGTAT CCAATAATGT TTGGAATCGA TGTGATATGC
    TTCCTCATCA TGACATTTGG ATACTCGGCA TTTGGTGAAG GAGGTTCTGG AAATGTTTTA
    GATGATGTGA AGGCTTCTCG TATTCCGGTA ACACTTGTTG TTATGCTCGT TGGAATGACT
    CTGGCAATTA TCATTGATCG AGCACTCTAC CTCAGAAAAT CAGTTGTCGG AAAGCTAATC
    TATCAAGTCT TAATGATTGC TTTCCTTCAC ATTTGGGTAT TCCTTGTTCT TCCAAATATG
    ACTCGTCGTT CTGCGATATC GAATCACGTT GCTCAAGCAT TGTACGTCAT CAAATCGTGC
    TATTTCCTTG TGTCTGCTTG GCAGATCCGA AATGGTTATC CTGAACTTTG TATTGGAAAT
    CTATTAACAC ATTCATACGG AATGACCAAT ATGATTGCAT TCAAAGTATT CATGAATATT
    CCATTCCTCT TCGAGCTTCG TACAGCTATT GATTGGACAT GGACAGATAC TTCAATGCCT
    CTTTTCGATT TCTTCAATAT GGAAAATTTC TATGCTCATA TATTCAATAT CAAGTGTGCC
    CGCCAGTTCG AAGCTGCCTA TCCAGCTCCA AGAGGGATTC CCAAAGGAAA GTTGGTCAAG
    TATATGATGG GATTCCCAAT TATCATTGGA GTCGTTATCT TCATCTTTTC TCCTCTATTG
    CTCTGGTCGC TACTCAATCA AATCGGAACT ATCAGTATGC CAGAAAAAGT TACGTTGAGG
    ATTTCGATTG AAGGATATCC ACCACTTTAC GAGATGGAGG CACAAGGAAG TAATCACGAT
    AACGCTGAAC TTGGAATGAT AAAACCTGAT CAATTGGCAT CTTTGAATCA AGCATTGACG
    GATAGTTACA CGACACGAGA CACTAATTCT ATTTTAAGAT CAAGAATGTC TGTATCATAT
    CTGAAAGGAT ACACATATGA GGATATTCTG ATTGTGCGAT TCCGTCCAGA ATCTGAAATT
    TATTGGCCGA TTTCACAGGA TTCGAGAAAT GCGATGATCG ATAAATTGAG CCGAAACACG
    TCAGTCAATT TTGAAGTATC TCTGGAATTC ACTCGGCCAT ATGATCCAAA TGAAAACGCT
    GCTTTAAAAC ATTCGAAATC GTGGCTTGTT CCAATCTCCC TGGATATGAC GATTCGAGCA
    AAAATTCAAA GTGCTCTCCG AGGAGATCCA GGTCATCCGA TTCTCATTCC ACAGTCAATT
    CCTGCGTTTA TTCAAGTTCC AAATCAAGGA GAATTGACCC TTCCAACATC AATCGGAAAT
    ACTATTATCA ATGACGGAAA TCCACGGATT AATACGACCG GGATGGAAAA ATCAGACGAA
    GCTCGGGCTT GGTTCGACTC GTTAACCTTG AATCTCGAAC AAGGAAAATC GCAAAACGAG
    AAAATGTGGA TCGCCACATC CGAACATCCA GGAGACCAAA ATGCGAAACT CTGGATAAAG
    ACTGCAAATA CGACGTATTC GGGAAGGCCG TACCTTCAAG TTGTCGGATT TATTGATAGA
    GCATTCCCAT CATTTTTGGC GAAAGTTTTC AAAGGAGGAG TCATTGCAGT GTATCTTTCA
    GTTATCCTCG TCGTTGGAAG AGGTCTGGTT CGTGGAATCT TCACCACATC TCCAAGCACT
    GTCATGTTCA CAGAATTACC GAATGCGGAT CATCTTCTCA AAATTTGTCT CGACATCTAT
    TTGGTTCGAG AAGCGAAGGA CTTCATGTTG GAGCAGGATC TCTTCGCCAA ATTAATATTC
    CTGTTCCGAT CACCAGCGAC ACTCATCGAA TGGACACGAA TGAGCAAAAA GAAGCAAGAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343007
    Clone ID Related Accession (Same CDS sequence) NM_001307050.1 , NM_001307050.2
    Accession Version NM_001307050.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGTTTCAGGA TCCAAAAAGT
    CGGAAGTTCG CGGCATTTGT GGAATATCTG TCGAATAAAG TGTCTGTCTA CTTCATATTT
    GTAGTATCAG TGGTACTTTT GGTGGTCTCT ACGTGTTTTG CTCCGAATTT CTACAATATT
    CTATTCTTCG CACTATGGGC ATTGAACTTG ATTTATCTCA AGTTCTCATT CCGTCTGTAT
    CGTGGATTGG CATATGCATT CTGGCTAACT CTCACGTTCT ACACTTCAAT TGTTATTATC
    GCCCTCTACA TTTATCAGTT CCCTGGAGTA TCTCAATGGA TTATTCGAAA TACTAGTTTG
    TCGCAGGAGT GGCTGAATGC AATTGGACTT GTCGATTTTA GAGCTATCGG TGAATCGGGA
    GCCCTATTTC TACAATTACT CGCCCCAATT GCCTTGTTCG TCGTCACAAT GCTCCAACTC
    AAGTTTTTCC ATGGACCATG GAGTCGAGCA ACGTCACCAA GAAGAGCAGA AAATGATCCA
    CCTACAAGTA CGACGGAGGC TGCGGCGGTA GCAAGCACAT CTGGAACACA AGGAAGAGCA
    CATGCTGCAG GCGATACCCT TGTCAAGAAG CTGCACAAAT TGGCAAATCA GACAATCGAA
    CTGTTATGGA GGTTTTTCGA AGTTCACATT TCGAAAATTG TCTTTGTCAT CATTGCGATT
    TTTATTGCCA ACAATATCAA TGCTCTATAC ATTCCACTTG TTATTCTCCT CTCATTGGCT
    ATATGTCTAC CATCAGCTGC AGATGGGATA TTCTCTTTAT TTATGTGTGC CTACTTATTC
    CTCGTGGCAC TGAGCAAGAT GATCTACCAA CTGGATATTG TTCCCGAGTT ATCACAAATC
    GACCGAGGAG TTGGAGCAGA TAACTGTTCA CATGGAAATA TTTCAATGCC TGAATGGTTT
    GGATTGAAGA AGGAAGTTGA AGGAACTGAA CCAATTTATA TGCTCTTTGG AGTCATTGTT
    TCGATTATTG CATTGGCTTT TCAATCAATT GTTATATATC GACAAAGACA TTATCGAGCT
    TCACTGGGAC TTCCTGAATC GATGCGAGCT AAAGTGTTCC CGGACTTCCA TCATTCTCAT
    TTTGATAGAT CTCTCAAAAA TGCCATCCAA TTCTTGATAG ACTATGGTTT CTATAAGTTT
    GGACTTGAAA TCACGATGAT CGCCATTGGA ATTGACATTT TCAATCGAAT GGATGCATTG
    GCAGCGATTC AATGTTTCTG GCTTGTCCTG TTTGCGCTCA ATAAAAGAGT CTTTGTTCGA
    AGAATATGGG TATTCTATGT GATCTATATG GCGATTCTGT ATCCACTTCA GTTCTTTTCA
    TATGTTGGAT TACCACCTGA CAGTTGTATT GAGTATCCAT GGTCGTATTG GATTCCATCG
    TATAGTGATG ATGCGAGATT CAATCTGTCG TATCTTCTGA ACTTGTCAAT ATACGGTGTA
    AACTGGCCAT CAGCATATCT CATTGGCGAT TTCTTCGTAC TCCTCTTGGC ATCATGCCAA
    TTAGCAGTTT TCCGGCGAGA AGGAGAAGAT AATGATTCAA TTTACAATGA TGGAAACTTT
    GTGATTAAGC CAGAAAATCC ACAATATGAC TTTATTGACA CCAAAAAGAG TTACGTTGAT
    TATTTCAAAT CATTCGTTTT CCATTATGGA CACTGGATTA CATTGATGTC TACACTAGCA
    GCAGGAATTG CTGGAACGTC ACTTTTTGCT TTGGGATACA TTATATTCAC TCTGACAATG
    CTGTGGTCTG GTAACAATTT GTATGTGATG AACTCAACTC TACGATCATT TGAACATACT
    TTGAAACGAT GGAATGCACT TTTGGGATAC ACATTGTTCA CGATTACAAT GAAAGTTTGC
    CTTCAAATAT TTGGATGTGT ATTCCTGTCG TGGTTCGATC AATCTGGTGG ATGGGGAAAG
    ACTCTGTGCA TCGTTCGTCA ACTCTTCAGT ATCACATGTG TTAATAACGA ATGTCATGTG
    CTCAAAGAGC TGGAGGATTT CTCGAAGGCG TGTGCTGTTG AGACGAAAGA AGGAAATATT
    GGATTTGATG TGATTGCGCT ATCCTTCCTC GTCTTCCAAA TCCGAATTTT CCATTCTTGG
    TATTTCCAAC ACTGCATGGT TGAATATCGA AGTGAGGTTA TTCTGGCCAA TAGAGGTGCC
    GTGCTGAAGA ATCAACTGAT TGAAAAGGAA ATGAAGGAAC AAAACGAGCA GCAAAAAGCC
    AAGTTCAATG ATATCAGAAG AAGAACTGAA GCTATTCGTG AAAGATATCA AAAACAAATC
    GAACGAGGAG CTGCTGAACG TGACTTCGAG CCGGTTACTT ATGGGCACGC CAAACGAGCG
    GGAGACTACT ACATGTTCAA ATACGATCCT GAAAATGATG ATCTTGTGGA ACCCGTCGAC
    AGTTTTGTAC CAGAAGTTGA TCCCAAGGCA ACTGCATATG ATCGACTTGA TCCTGGACAG
    ATAATGTACG CTGCCACCGC TCACGACCTC GACCTCGCCA AAACTGTACA GCAAGTCAAG
    AAAGGCGATA CTATCAAAGA TCCTGATTCA CGTGCTCTTA TCGCTGTTTC TGAACCAGAA
    GCTCGTAAGC CAGGAGGCAC TGAAGAAACG GATGGTGATG AAGATGAGGA TAATAAGGAT
    TCGAAAGTGG AAAGTACTGC TAAATTCATT CAAAAAATGA TCGCTTCTGC CTTGGACTTG
    TGTAGTGTAA CACTAAACAA ACTGTGCAGA GAACACCGAT ATGTTGGATT TGTTTTGAGC
    AAGGAAAAAC AAAAGTTGAA GTCAGGACAC TCGGAATCTC TTTCAAATAC ATCTAGAAAG
    CTGACGGATA TTCGATCTGC AGTTGATCTA CCAAGTCTTC AACTTGTGCA ATCGGCGAAT
    GATGTTGAAA AGATGGAGAC AGCCGTCAGC GTGGATTGGC AACAAAAATC ATCAGCAACG
    AGACTATTGA ATGCAGTTGT GAATTGTATT GGAGCACACA CTGATATTCT CTGCTACTTC
    TTCGCAATTA TGACACAAGT GATGACTGGT GGACTTATTA CTCTTCCACT TCCTCTGATG
    TCACTATTCT GGGGAAATCT ATCGAATCCT CGTCCATCCA AGTTCTTCTG GGTCACAATG
    ATCACCTACA CTGAGTGTGT CATTGTTATC AAGTTTGTTT GCCAATTTGC ATTCATGCCT
    TACAATTCTA TCACGTGGCG AACAGAGCAT CAAATGGACC CGATGTCATT GGATAAGTTA
    TTCGGAGTAT CACAGAGAGA TAGTTTTGCA CTTTGGGACA TTGTTTTGCT CTTCTCACTC
    TTCTTCCATC GGTATATGCT CAGAAAGTTA GGGTTATGGA AGGATGCCAA TTTGACGGAC
    ACGTTCACTT TGAAGGAAGA GCCTCGATCG GCGTCAGGAT CGGACACAGG ATCACCCAAG
    AAAATTGCTC AAGAGCCAAA GGTTGTTGTA ACTCAGTCAG ATACCCTTGA AGGTACAAGT
    GGAGGAGAGA TAGTCATACC ATCTGATCCA AATGCAGTTT CCAATATGGA AGAATTAGAC
    TGCGAACCTC CGATTCCAGA GAAGCAAAGT GGACCAATTG GAAGATTCAT TCATCAATTA
    TTCCATCCAA AATTCCGTTA CATTCGTGAT TTGTATCCAA TAATGTTTGG AATCGATGTG
    ATATGCTTCC TCATCATGAC ATTTGGATAC TCGGCATTTG GTGAAGGAGG TTCTGGAAAT
    GTTTTAGATG ATGTGAAGGC TTCTCGTATT CCGGTAACAC TTGTTGTTAT GCTCGTTGGA
    ATGACTCTGG CAATTATCAT TGATCGAGCA CTCTACCTCA GAAAATCAGT TGTCGGAAAG
    CTAATCTATC AAGTCTTAAT GATTGCTTTC CTTCACATTT GGGTATTCCT TGTTCTTCCA
    AATATGACTC GTCGTTCTGC GATATCGAAT CACGTTGCTC AAGCATTGTA CGTCATCAAA
    TCGTGCTATT TCCTTGTGTC TGCTTGGCAG ATCCGAAATG GTTATCCTGA ACTTTGTATT
    GGAAATCTAT TAACACATTC ATACGGAATG ACCAATATGA TTGCATTCAA AGTATTCATG
    AATATTCCAT TCCTCTTCGA GCTTCGTACA GCTATTGATT GGACATGGAC AGATACTTCA
    ATGCCTCTTT TCGATTTCTT CAATATGGAA AATTTCTATG CTCATATATT CAATATCAAG
    TGTGCCCGCC AGTTCGAAGC TGCCTATCCA GCTCCAAGAG GGATTCCCAA AGGAAAGTTG
    GTCAAGTATA TGATGGGATT CCCAATTATC ATTGGAGTCG TTATCTTCAT CTTTTCTCCT
    CTATTGCTCT GGTCGCTACT CAATCAAATC GGAACTATCA GTATGCCAGA AAAAGTTACG
    TTGAGGATTT CGATTGAAGG ATATCCACCA CTTTACGAGA TGGAGGCACA AGGAAGTAAT
    CACGATAACG CTGAACTTGG AATGATAAAA CCTGATCAAT TGGCATCTTT GAATCAAGCA
    TTGACGGATA GTTACACGAC ACGAGACACT AATTCTATTT TAAGATCAAG AATGTCTGTA
    TCATATCTGA AAGGATACAC ATATGAGGAT ATTCTGATTG TGCGATTCCG TCCAGAATCT
    GAAATTTATT GGCCGATTTC ACAGGATTCG AGAAATGCGA TGATCGATAA ATTGAGCCGA
    AACACGTCAG TCAATTTTGA AGTATCTCTG GAATTCACTC GGCCATATGA TCCAAATGAA
    AACGCTGCTT TAAAACATTC GAAATCGTGG CTTGTTCCAA TCTCCCTGGA TATGACGATT
    CGAGCAAAAA TTCAAAGTGC TCTCCGAGGA GATCCAGGTC ATCCGATTCT CATTCCACAG
    TCAATTCCTG CGTTTATTCA AGTTCCAAAT CAAGGAGAAT TGACCCTTCC AACATCAATC
    GGAAATACTA TTATCAATGA CGGAAATCCA CGGATTAATA CGACCGGGAT GGAAAAATCA
    GACGAAGCTC GGGCTTGGTT CGACTCGTTA ACCTTGAATC TCGAACAAGG AAAATCGCAA
    AACGAGAAAA TGTGGATCGC CACATCCGAA CATCCAGGAG ACCAAAATGC GAAACTCTGG
    ATAAAGACTG CAAATACGAC GTATTCGGGA AGGCCGTACC TTCAAGTTGT CGGATTTATT
    GATAGAGCAT TCCCATCATT TTTGGCGAAA GTTTTCAAAG GAGGAGTCAT TGCAGTGTAT
    CTTTCAGTTA TCCTCGTCGT TGGAAGAGGT CTGGTTCGTG GAATCTTCAC CACATCTCCA
    AGCACTGTCA TGTTCACAGA ATTACCGAAT GCGGATCATC TTCTCAAAAT TTGTCTCGAC
    ATCTATTTGG TTCGAGAAGC GAAGGACTTC ATGTTGGAGC AGGATCTCTT CGCCAAATTA
    ATATTCCTGT TCCGATCACC AGCGACACTC ATCGAATGGA CACGAATGAG CAAAAAGAAG
    CAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293979.1
    CDS1..7209
    Translation

    Target ORF information:

    RefSeq Version NM_001307050.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGTTTCAGGA TCCAAAAAGT
    CGGAAGTTCG CGGCATTTGT GGAATATCTG TCGAATAAAG TGTCTGTCTA CTTCATATTT
    GTAGTATCAG TGGTACTTTT GGTGGTCTCT ACGTGTTTTG CTCCGAATTT CTACAATATT
    CTATTCTTCG CACTATGGGC ATTGAACTTG ATTTATCTCA AGTTCTCATT CCGTCTGTAT
    CGTGGATTGG CATATGCATT CTGGCTAACT CTCACGTTCT ACACTTCAAT TGTTATTATC
    GCCCTCTACA TTTATCAGTT CCCTGGAGTA TCTCAATGGA TTATTCGAAA TACTAGTTTG
    TCGCAGGAGT GGCTGAATGC AATTGGACTT GTCGATTTTA GAGCTATCGG TGAATCGGGA
    GCCCTATTTC TACAATTACT CGCCCCAATT GCCTTGTTCG TCGTCACAAT GCTCCAACTC
    AAGTTTTTCC ATGGACCATG GAGTCGAGCA ACGTCACCAA GAAGAGCAGA AAATGATCCA
    CCTACAAGTA CGACGGAGGC TGCGGCGGTA GCAAGCACAT CTGGAACACA AGGAAGAGCA
    CATGCTGCAG GCGATACCCT TGTCAAGAAG CTGCACAAAT TGGCAAATCA GACAATCGAA
    CTGTTATGGA GGTTTTTCGA AGTTCACATT TCGAAAATTG TCTTTGTCAT CATTGCGATT
    TTTATTGCCA ACAATATCAA TGCTCTATAC ATTCCACTTG TTATTCTCCT CTCATTGGCT
    ATATGTCTAC CATCAGCTGC AGATGGGATA TTCTCTTTAT TTATGTGTGC CTACTTATTC
    CTCGTGGCAC TGAGCAAGAT GATCTACCAA CTGGATATTG TTCCCGAGTT ATCACAAATC
    GACCGAGGAG TTGGAGCAGA TAACTGTTCA CATGGAAATA TTTCAATGCC TGAATGGTTT
    GGATTGAAGA AGGAAGTTGA AGGAACTGAA CCAATTTATA TGCTCTTTGG AGTCATTGTT
    TCGATTATTG CATTGGCTTT TCAATCAATT GTTATATATC GACAAAGACA TTATCGAGCT
    TCACTGGGAC TTCCTGAATC GATGCGAGCT AAAGTGTTCC CGGACTTCCA TCATTCTCAT
    TTTGATAGAT CTCTCAAAAA TGCCATCCAA TTCTTGATAG ACTATGGTTT CTATAAGTTT
    GGACTTGAAA TCACGATGAT CGCCATTGGA ATTGACATTT TCAATCGAAT GGATGCATTG
    GCAGCGATTC AATGTTTCTG GCTTGTCCTG TTTGCGCTCA ATAAAAGAGT CTTTGTTCGA
    AGAATATGGG TATTCTATGT GATCTATATG GCGATTCTGT ATCCACTTCA GTTCTTTTCA
    TATGTTGGAT TACCACCTGA CAGTTGTATT GAGTATCCAT GGTCGTATTG GATTCCATCG
    TATAGTGATG ATGCGAGATT CAATCTGTCG TATCTTCTGA ACTTGTCAAT ATACGGTGTA
    AACTGGCCAT CAGCATATCT CATTGGCGAT TTCTTCGTAC TCCTCTTGGC ATCATGCCAA
    TTAGCAGTTT TCCGGCGAGA AGGAGAAGAT AATGATTCAA TTTACAATGA TGGAAACTTT
    GTGATTAAGC CAGAAAATCC ACAATATGAC TTTATTGACA CCAAAAAGAG TTACGTTGAT
    TATTTCAAAT CATTCGTTTT CCATTATGGA CACTGGATTA CATTGATGTC TACACTAGCA
    GCAGGAATTG CTGGAACGTC ACTTTTTGCT TTGGGATACA TTATATTCAC TCTGACAATG
    CTGTGGTCTG GTAACAATTT GTATGTGATG AACTCAACTC TACGATCATT TGAACATACT
    TTGAAACGAT GGAATGCACT TTTGGGATAC ACATTGTTCA CGATTACAAT GAAAGTTTGC
    CTTCAAATAT TTGGATGTGT ATTCCTGTCG TGGTTCGATC AATCTGGTGG ATGGGGAAAG
    ACTCTGTGCA TCGTTCGTCA ACTCTTCAGT ATCACATGTG TTAATAACGA ATGTCATGTG
    CTCAAAGAGC TGGAGGATTT CTCGAAGGCG TGTGCTGTTG AGACGAAAGA AGGAAATATT
    GGATTTGATG TGATTGCGCT ATCCTTCCTC GTCTTCCAAA TCCGAATTTT CCATTCTTGG
    TATTTCCAAC ACTGCATGGT TGAATATCGA AGTGAGGTTA TTCTGGCCAA TAGAGGTGCC
    GTGCTGAAGA ATCAACTGAT TGAAAAGGAA ATGAAGGAAC AAAACGAGCA GCAAAAAGCC
    AAGTTCAATG ATATCAGAAG AAGAACTGAA GCTATTCGTG AAAGATATCA AAAACAAATC
    GAACGAGGAG CTGCTGAACG TGACTTCGAG CCGGTTACTT ATGGGCACGC CAAACGAGCG
    GGAGACTACT ACATGTTCAA ATACGATCCT GAAAATGATG ATCTTGTGGA ACCCGTCGAC
    AGTTTTGTAC CAGAAGTTGA TCCCAAGGCA ACTGCATATG ATCGACTTGA TCCTGGACAG
    ATAATGTACG CTGCCACCGC TCACGACCTC GACCTCGCCA AAACTGTACA GCAAGTCAAG
    AAAGGCGATA CTATCAAAGA TCCTGATTCA CGTGCTCTTA TCGCTGTTTC TGAACCAGAA
    GCTCGTAAGC CAGGAGGCAC TGAAGAAACG GATGGTGATG AAGATGAGGA TAATAAGGAT
    TCGAAAGTGG AAAGTACTGC TAAATTCATT CAAAAAATGA TCGCTTCTGC CTTGGACTTG
    TGTAGTGTAA CACTAAACAA ACTGTGCAGA GAACACCGAT ATGTTGGATT TGTTTTGAGC
    AAGGAAAAAC AAAAGTTGAA GTCAGGACAC TCGGAATCTC TTTCAAATAC ATCTAGAAAG
    CTGACGGATA TTCGATCTGC AGTTGATCTA CCAAGTCTTC AACTTGTGCA ATCGGCGAAT
    GATGTTGAAA AGATGGAGAC AGCCGTCAGC GTGGATTGGC AACAAAAATC ATCAGCAACG
    AGACTATTGA ATGCAGTTGT GAATTGTATT GGAGCACACA CTGATATTCT CTGCTACTTC
    TTCGCAATTA TGACACAAGT GATGACTGGT GGACTTATTA CTCTTCCACT TCCTCTGATG
    TCACTATTCT GGGGAAATCT ATCGAATCCT CGTCCATCCA AGTTCTTCTG GGTCACAATG
    ATCACCTACA CTGAGTGTGT CATTGTTATC AAGTTTGTTT GCCAATTTGC ATTCATGCCT
    TACAATTCTA TCACGTGGCG AACAGAGCAT CAAATGGACC CGATGTCATT GGATAAGTTA
    TTCGGAGTAT CACAGAGAGA TAGTTTTGCA CTTTGGGACA TTGTTTTGCT CTTCTCACTC
    TTCTTCCATC GGTATATGCT CAGAAAGTTA GGGTTATGGA AGGATGCCAA TTTGACGGAC
    ACGTTCACTT TGAAGGAAGA GCCTCGATCG GCGTCAGGAT CGGACACAGG ATCACCCAAG
    AAAATTGCTC AAGAGCCAAA GGTTGTTGTA ACTCAGTCAG ATACCCTTGA AGGTACAAGT
    GGAGGAGAGA TAGTCATACC ATCTGATCCA AATGCAGTTT CCAATATGGA AGAATTAGAC
    TGCGAACCTC CGATTCCAGA GAAGCAAAGT GGACCAATTG GAAGATTCAT TCATCAATTA
    TTCCATCCAA AATTCCGTTA CATTCGTGAT TTGTATCCAA TAATGTTTGG AATCGATGTG
    ATATGCTTCC TCATCATGAC ATTTGGATAC TCGGCATTTG GTGAAGGAGG TTCTGGAAAT
    GTTTTAGATG ATGTGAAGGC TTCTCGTATT CCGGTAACAC TTGTTGTTAT GCTCGTTGGA
    ATGACTCTGG CAATTATCAT TGATCGAGCA CTCTACCTCA GAAAATCAGT TGTCGGAAAG
    CTAATCTATC AAGTCTTAAT GATTGCTTTC CTTCACATTT GGGTATTCCT TGTTCTTCCA
    AATATGACTC GTCGTTCTGC GATATCGAAT CACGTTGCTC AAGCATTGTA CGTCATCAAA
    TCGTGCTATT TCCTTGTGTC TGCTTGGCAG ATCCGAAATG GTTATCCTGA ACTTTGTATT
    GGAAATCTAT TAACACATTC ATACGGAATG ACCAATATGA TTGCATTCAA AGTATTCATG
    AATATTCCAT TCCTCTTCGA GCTTCGTACA GCTATTGATT GGACATGGAC AGATACTTCA
    ATGCCTCTTT TCGATTTCTT CAATATGGAA AATTTCTATG CTCATATATT CAATATCAAG
    TGTGCCCGCC AGTTCGAAGC TGCCTATCCA GCTCCAAGAG GGATTCCCAA AGGAAAGTTG
    GTCAAGTATA TGATGGGATT CCCAATTATC ATTGGAGTCG TTATCTTCAT CTTTTCTCCT
    CTATTGCTCT GGTCGCTACT CAATCAAATC GGAACTATCA GTATGCCAGA AAAAGTTACG
    TTGAGGATTT CGATTGAAGG ATATCCACCA CTTTACGAGA TGGAGGCACA AGGAAGTAAT
    CACGATAACG CTGAACTTGG AATGATAAAA CCTGATCAAT TGGCATCTTT GAATCAAGCA
    TTGACGGATA GTTACACGAC ACGAGACACT AATTCTATTT TAAGATCAAG AATGTCTGTA
    TCATATCTGA AAGGATACAC ATATGAGGAT ATTCTGATTG TGCGATTCCG TCCAGAATCT
    GAAATTTATT GGCCGATTTC ACAGGATTCG AGAAATGCGA TGATCGATAA ATTGAGCCGA
    AACACGTCAG TCAATTTTGA AGTATCTCTG GAATTCACTC GGCCATATGA TCCAAATGAA
    AACGCTGCTT TAAAACATTC GAAATCGTGG CTTGTTCCAA TCTCCCTGGA TATGACGATT
    CGAGCAAAAA TTCAAAGTGC TCTCCGAGGA GATCCAGGTC ATCCGATTCT CATTCCACAG
    TCAATTCCTG CGTTTATTCA AGTTCCAAAT CAAGGAGAAT TGACCCTTCC AACATCAATC
    GGAAATACTA TTATCAATGA CGGAAATCCA CGGATTAATA CGACCGGGAT GGAAAAATCA
    GACGAAGCTC GGGCTTGGTT CGACTCGTTA ACCTTGAATC TCGAACAAGG AAAATCGCAA
    AACGAGAAAA TGTGGATCGC CACATCCGAA CATCCAGGAG ACCAAAATGC GAAACTCTGG
    ATAAAGACTG CAAATACGAC GTATTCGGGA AGGCCGTACC TTCAAGTTGT CGGATTTATT
    GATAGAGCAT TCCCATCATT TTTGGCGAAA GTTTTCAAAG GAGGAGTCAT TGCAGTGTAT
    CTTTCAGTTA TCCTCGTCGT TGGAAGAGGT CTGGTTCGTG GAATCTTCAC CACATCTCCA
    AGCACTGTCA TGTTCACAGA ATTACCGAAT GCGGATCATC TTCTCAAAAT TTGTCTCGAC
    ATCTATTTGG TTCGAGAAGC GAAGGACTTC ATGTTGGAGC AGGATCTCTT CGCCAAATTA
    ATATTCCTGT TCCGATCACC AGCGACACTC ATCGAATGGA CACGAATGAG CAAAAAGAAG
    CAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343007
    Clone ID Related Accession (Same CDS sequence) NM_001307050.1 , NM_001307050.2
    Accession Version NM_001307050.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307050.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGTTTCAGGA TCCAAAAAGT
    CGGAAGTTCG CGGCATTTGT GGAATATCTG TCGAATAAAG TGTCTGTCTA CTTCATATTT
    GTAGTATCAG TGGTACTTTT GGTGGTCTCT ACGTGTTTTG CTCCGAATTT CTACAATATT
    CTATTCTTCG CACTATGGGC ATTGAACTTG ATTTATCTCA AGTTCTCATT CCGTCTGTAT
    CGTGGATTGG CATATGCATT CTGGCTAACT CTCACGTTCT ACACTTCAAT TGTTATTATC
    GCCCTCTACA TTTATCAGTT CCCTGGAGTA TCTCAATGGA TTATTCGAAA TACTAGTTTG
    TCGCAGGAGT GGCTGAATGC AATTGGACTT GTCGATTTTA GAGCTATCGG TGAATCGGGA
    GCCCTATTTC TACAATTACT CGCCCCAATT GCCTTGTTCG TCGTCACAAT GCTCCAACTC
    AAGTTTTTCC ATGGACCATG GAGTCGAGCA ACGTCACCAA GAAGAGCAGA AAATGATCCA
    CCTACAAGTA CGACGGAGGC TGCGGCGGTA GCAAGCACAT CTGGAACACA AGGAAGAGCA
    CATGCTGCAG GCGATACCCT TGTCAAGAAG CTGCACAAAT TGGCAAATCA GACAATCGAA
    CTGTTATGGA GGTTTTTCGA AGTTCACATT TCGAAAATTG TCTTTGTCAT CATTGCGATT
    TTTATTGCCA ACAATATCAA TGCTCTATAC ATTCCACTTG TTATTCTCCT CTCATTGGCT
    ATATGTCTAC CATCAGCTGC AGATGGGATA TTCTCTTTAT TTATGTGTGC CTACTTATTC
    CTCGTGGCAC TGAGCAAGAT GATCTACCAA CTGGATATTG TTCCCGAGTT ATCACAAATC
    GACCGAGGAG TTGGAGCAGA TAACTGTTCA CATGGAAATA TTTCAATGCC TGAATGGTTT
    GGATTGAAGA AGGAAGTTGA AGGAACTGAA CCAATTTATA TGCTCTTTGG AGTCATTGTT
    TCGATTATTG CATTGGCTTT TCAATCAATT GTTATATATC GACAAAGACA TTATCGAGCT
    TCACTGGGAC TTCCTGAATC GATGCGAGCT AAAGTGTTCC CGGACTTCCA TCATTCTCAT
    TTTGATAGAT CTCTCAAAAA TGCCATCCAA TTCTTGATAG ACTATGGTTT CTATAAGTTT
    GGACTTGAAA TCACGATGAT CGCCATTGGA ATTGACATTT TCAATCGAAT GGATGCATTG
    GCAGCGATTC AATGTTTCTG GCTTGTCCTG TTTGCGCTCA ATAAAAGAGT CTTTGTTCGA
    AGAATATGGG TATTCTATGT GATCTATATG GCGATTCTGT ATCCACTTCA GTTCTTTTCA
    TATGTTGGAT TACCACCTGA CAGTTGTATT GAGTATCCAT GGTCGTATTG GATTCCATCG
    TATAGTGATG ATGCGAGATT CAATCTGTCG TATCTTCTGA ACTTGTCAAT ATACGGTGTA
    AACTGGCCAT CAGCATATCT CATTGGCGAT TTCTTCGTAC TCCTCTTGGC ATCATGCCAA
    TTAGCAGTTT TCCGGCGAGA AGGAGAAGAT AATGATTCAA TTTACAATGA TGGAAACTTT
    GTGATTAAGC CAGAAAATCC ACAATATGAC TTTATTGACA CCAAAAAGAG TTACGTTGAT
    TATTTCAAAT CATTCGTTTT CCATTATGGA CACTGGATTA CATTGATGTC TACACTAGCA
    GCAGGAATTG CTGGAACGTC ACTTTTTGCT TTGGGATACA TTATATTCAC TCTGACAATG
    CTGTGGTCTG GTAACAATTT GTATGTGATG AACTCAACTC TACGATCATT TGAACATACT
    TTGAAACGAT GGAATGCACT TTTGGGATAC ACATTGTTCA CGATTACAAT GAAAGTTTGC
    CTTCAAATAT TTGGATGTGT ATTCCTGTCG TGGTTCGATC AATCTGGTGG ATGGGGAAAG
    ACTCTGTGCA TCGTTCGTCA ACTCTTCAGT ATCACATGTG TTAATAACGA ATGTCATGTG
    CTCAAAGAGC TGGAGGATTT CTCGAAGGCG TGTGCTGTTG AGACGAAAGA AGGAAATATT
    GGATTTGATG TGATTGCGCT ATCCTTCCTC GTCTTCCAAA TCCGAATTTT CCATTCTTGG
    TATTTCCAAC ACTGCATGGT TGAATATCGA AGTGAGGTTA TTCTGGCCAA TAGAGGTGCC
    GTGCTGAAGA ATCAACTGAT TGAAAAGGAA ATGAAGGAAC AAAACGAGCA GCAAAAAGCC
    AAGTTCAATG ATATCAGAAG AAGAACTGAA GCTATTCGTG AAAGATATCA AAAACAAATC
    GAACGAGGAG CTGCTGAACG TGACTTCGAG CCGGTTACTT ATGGGCACGC CAAACGAGCG
    GGAGACTACT ACATGTTCAA ATACGATCCT GAAAATGATG ATCTTGTGGA ACCCGTCGAC
    AGTTTTGTAC CAGAAGTTGA TCCCAAGGCA ACTGCATATG ATCGACTTGA TCCTGGACAG
    ATAATGTACG CTGCCACCGC TCACGACCTC GACCTCGCCA AAACTGTACA GCAAGTCAAG
    AAAGGCGATA CTATCAAAGA TCCTGATTCA CGTGCTCTTA TCGCTGTTTC TGAACCAGAA
    GCTCGTAAGC CAGGAGGCAC TGAAGAAACG GATGGTGATG AAGATGAGGA TAATAAGGAT
    TCGAAAGTGG AAAGTACTGC TAAATTCATT CAAAAAATGA TCGCTTCTGC CTTGGACTTG
    TGTAGTGTAA CACTAAACAA ACTGTGCAGA GAACACCGAT ATGTTGGATT TGTTTTGAGC
    AAGGAAAAAC AAAAGTTGAA GTCAGGACAC TCGGAATCTC TTTCAAATAC ATCTAGAAAG
    CTGACGGATA TTCGATCTGC AGTTGATCTA CCAAGTCTTC AACTTGTGCA ATCGGCGAAT
    GATGTTGAAA AGATGGAGAC AGCCGTCAGC GTGGATTGGC AACAAAAATC ATCAGCAACG
    AGACTATTGA ATGCAGTTGT GAATTGTATT GGAGCACACA CTGATATTCT CTGCTACTTC
    TTCGCAATTA TGACACAAGT GATGACTGGT GGACTTATTA CTCTTCCACT TCCTCTGATG
    TCACTATTCT GGGGAAATCT ATCGAATCCT CGTCCATCCA AGTTCTTCTG GGTCACAATG
    ATCACCTACA CTGAGTGTGT CATTGTTATC AAGTTTGTTT GCCAATTTGC ATTCATGCCT
    TACAATTCTA TCACGTGGCG AACAGAGCAT CAAATGGACC CGATGTCATT GGATAAGTTA
    TTCGGAGTAT CACAGAGAGA TAGTTTTGCA CTTTGGGACA TTGTTTTGCT CTTCTCACTC
    TTCTTCCATC GGTATATGCT CAGAAAGTTA GGGTTATGGA AGGATGCCAA TTTGACGGAC
    ACGTTCACTT TGAAGGAAGA GCCTCGATCG GCGTCAGGAT CGGACACAGG ATCACCCAAG
    AAAATTGCTC AAGAGCCAAA GGTTGTTGTA ACTCAGTCAG ATACCCTTGA AGGTACAAGT
    GGAGGAGAGA TAGTCATACC ATCTGATCCA AATGCAGTTT CCAATATGGA AGAATTAGAC
    TGCGAACCTC CGATTCCAGA GAAGCAAAGT GGACCAATTG GAAGATTCAT TCATCAATTA
    TTCCATCCAA AATTCCGTTA CATTCGTGAT TTGTATCCAA TAATGTTTGG AATCGATGTG
    ATATGCTTCC TCATCATGAC ATTTGGATAC TCGGCATTTG GTGAAGGAGG TTCTGGAAAT
    GTTTTAGATG ATGTGAAGGC TTCTCGTATT CCGGTAACAC TTGTTGTTAT GCTCGTTGGA
    ATGACTCTGG CAATTATCAT TGATCGAGCA CTCTACCTCA GAAAATCAGT TGTCGGAAAG
    CTAATCTATC AAGTCTTAAT GATTGCTTTC CTTCACATTT GGGTATTCCT TGTTCTTCCA
    AATATGACTC GTCGTTCTGC GATATCGAAT CACGTTGCTC AAGCATTGTA CGTCATCAAA
    TCGTGCTATT TCCTTGTGTC TGCTTGGCAG ATCCGAAATG GTTATCCTGA ACTTTGTATT
    GGAAATCTAT TAACACATTC ATACGGAATG ACCAATATGA TTGCATTCAA AGTATTCATG
    AATATTCCAT TCCTCTTCGA GCTTCGTACA GCTATTGATT GGACATGGAC AGATACTTCA
    ATGCCTCTTT TCGATTTCTT CAATATGGAA AATTTCTATG CTCATATATT CAATATCAAG
    TGTGCCCGCC AGTTCGAAGC TGCCTATCCA GCTCCAAGAG GGATTCCCAA AGGAAAGTTG
    GTCAAGTATA TGATGGGATT CCCAATTATC ATTGGAGTCG TTATCTTCAT CTTTTCTCCT
    CTATTGCTCT GGTCGCTACT CAATCAAATC GGAACTATCA GTATGCCAGA AAAAGTTACG
    TTGAGGATTT CGATTGAAGG ATATCCACCA CTTTACGAGA TGGAGGCACA AGGAAGTAAT
    CACGATAACG CTGAACTTGG AATGATAAAA CCTGATCAAT TGGCATCTTT GAATCAAGCA
    TTGACGGATA GTTACACGAC ACGAGACACT AATTCTATTT TAAGATCAAG AATGTCTGTA
    TCATATCTGA AAGGATACAC ATATGAGGAT ATTCTGATTG TGCGATTCCG TCCAGAATCT
    GAAATTTATT GGCCGATTTC ACAGGATTCG AGAAATGCGA TGATCGATAA ATTGAGCCGA
    AACACGTCAG TCAATTTTGA AGTATCTCTG GAATTCACTC GGCCATATGA TCCAAATGAA
    AACGCTGCTT TAAAACATTC GAAATCGTGG CTTGTTCCAA TCTCCCTGGA TATGACGATT
    CGAGCAAAAA TTCAAAGTGC TCTCCGAGGA GATCCAGGTC ATCCGATTCT CATTCCACAG
    TCAATTCCTG CGTTTATTCA AGTTCCAAAT CAAGGAGAAT TGACCCTTCC AACATCAATC
    GGAAATACTA TTATCAATGA CGGAAATCCA CGGATTAATA CGACCGGGAT GGAAAAATCA
    GACGAAGCTC GGGCTTGGTT CGACTCGTTA ACCTTGAATC TCGAACAAGG AAAATCGCAA
    AACGAGAAAA TGTGGATCGC CACATCCGAA CATCCAGGAG ACCAAAATGC GAAACTCTGG
    ATAAAGACTG CAAATACGAC GTATTCGGGA AGGCCGTACC TTCAAGTTGT CGGATTTATT
    GATAGAGCAT TCCCATCATT TTTGGCGAAA GTTTTCAAAG GAGGAGTCAT TGCAGTGTAT
    CTTTCAGTTA TCCTCGTCGT TGGAAGAGGT CTGGTTCGTG GAATCTTCAC CACATCTCCA
    AGCACTGTCA TGTTCACAGA ATTACCGAAT GCGGATCATC TTCTCAAAAT TTGTCTCGAC
    ATCTATTTGG TTCGAGAAGC GAAGGACTTC ATGTTGGAGC AGGATCTCTT CGCCAAATTA
    ATATTCCTGT TCCGATCACC AGCGACACTC ATCGAATGGA CACGAATGAG CAAAAAGAAG
    CAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293979.1
    CDS57..7265
    Translation

    Target ORF information:

    RefSeq Version NM_001307050.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307050.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGTTTCAGGA TCCAAAAAGT
    CGGAAGTTCG CGGCATTTGT GGAATATCTG TCGAATAAAG TGTCTGTCTA CTTCATATTT
    GTAGTATCAG TGGTACTTTT GGTGGTCTCT ACGTGTTTTG CTCCGAATTT CTACAATATT
    CTATTCTTCG CACTATGGGC ATTGAACTTG ATTTATCTCA AGTTCTCATT CCGTCTGTAT
    CGTGGATTGG CATATGCATT CTGGCTAACT CTCACGTTCT ACACTTCAAT TGTTATTATC
    GCCCTCTACA TTTATCAGTT CCCTGGAGTA TCTCAATGGA TTATTCGAAA TACTAGTTTG
    TCGCAGGAGT GGCTGAATGC AATTGGACTT GTCGATTTTA GAGCTATCGG TGAATCGGGA
    GCCCTATTTC TACAATTACT CGCCCCAATT GCCTTGTTCG TCGTCACAAT GCTCCAACTC
    AAGTTTTTCC ATGGACCATG GAGTCGAGCA ACGTCACCAA GAAGAGCAGA AAATGATCCA
    CCTACAAGTA CGACGGAGGC TGCGGCGGTA GCAAGCACAT CTGGAACACA AGGAAGAGCA
    CATGCTGCAG GCGATACCCT TGTCAAGAAG CTGCACAAAT TGGCAAATCA GACAATCGAA
    CTGTTATGGA GGTTTTTCGA AGTTCACATT TCGAAAATTG TCTTTGTCAT CATTGCGATT
    TTTATTGCCA ACAATATCAA TGCTCTATAC ATTCCACTTG TTATTCTCCT CTCATTGGCT
    ATATGTCTAC CATCAGCTGC AGATGGGATA TTCTCTTTAT TTATGTGTGC CTACTTATTC
    CTCGTGGCAC TGAGCAAGAT GATCTACCAA CTGGATATTG TTCCCGAGTT ATCACAAATC
    GACCGAGGAG TTGGAGCAGA TAACTGTTCA CATGGAAATA TTTCAATGCC TGAATGGTTT
    GGATTGAAGA AGGAAGTTGA AGGAACTGAA CCAATTTATA TGCTCTTTGG AGTCATTGTT
    TCGATTATTG CATTGGCTTT TCAATCAATT GTTATATATC GACAAAGACA TTATCGAGCT
    TCACTGGGAC TTCCTGAATC GATGCGAGCT AAAGTGTTCC CGGACTTCCA TCATTCTCAT
    TTTGATAGAT CTCTCAAAAA TGCCATCCAA TTCTTGATAG ACTATGGTTT CTATAAGTTT
    GGACTTGAAA TCACGATGAT CGCCATTGGA ATTGACATTT TCAATCGAAT GGATGCATTG
    GCAGCGATTC AATGTTTCTG GCTTGTCCTG TTTGCGCTCA ATAAAAGAGT CTTTGTTCGA
    AGAATATGGG TATTCTATGT GATCTATATG GCGATTCTGT ATCCACTTCA GTTCTTTTCA
    TATGTTGGAT TACCACCTGA CAGTTGTATT GAGTATCCAT GGTCGTATTG GATTCCATCG
    TATAGTGATG ATGCGAGATT CAATCTGTCG TATCTTCTGA ACTTGTCAAT ATACGGTGTA
    AACTGGCCAT CAGCATATCT CATTGGCGAT TTCTTCGTAC TCCTCTTGGC ATCATGCCAA
    TTAGCAGTTT TCCGGCGAGA AGGAGAAGAT AATGATTCAA TTTACAATGA TGGAAACTTT
    GTGATTAAGC CAGAAAATCC ACAATATGAC TTTATTGACA CCAAAAAGAG TTACGTTGAT
    TATTTCAAAT CATTCGTTTT CCATTATGGA CACTGGATTA CATTGATGTC TACACTAGCA
    GCAGGAATTG CTGGAACGTC ACTTTTTGCT TTGGGATACA TTATATTCAC TCTGACAATG
    CTGTGGTCTG GTAACAATTT GTATGTGATG AACTCAACTC TACGATCATT TGAACATACT
    TTGAAACGAT GGAATGCACT TTTGGGATAC ACATTGTTCA CGATTACAAT GAAAGTTTGC
    CTTCAAATAT TTGGATGTGT ATTCCTGTCG TGGTTCGATC AATCTGGTGG ATGGGGAAAG
    ACTCTGTGCA TCGTTCGTCA ACTCTTCAGT ATCACATGTG TTAATAACGA ATGTCATGTG
    CTCAAAGAGC TGGAGGATTT CTCGAAGGCG TGTGCTGTTG AGACGAAAGA AGGAAATATT
    GGATTTGATG TGATTGCGCT ATCCTTCCTC GTCTTCCAAA TCCGAATTTT CCATTCTTGG
    TATTTCCAAC ACTGCATGGT TGAATATCGA AGTGAGGTTA TTCTGGCCAA TAGAGGTGCC
    GTGCTGAAGA ATCAACTGAT TGAAAAGGAA ATGAAGGAAC AAAACGAGCA GCAAAAAGCC
    AAGTTCAATG ATATCAGAAG AAGAACTGAA GCTATTCGTG AAAGATATCA AAAACAAATC
    GAACGAGGAG CTGCTGAACG TGACTTCGAG CCGGTTACTT ATGGGCACGC CAAACGAGCG
    GGAGACTACT ACATGTTCAA ATACGATCCT GAAAATGATG ATCTTGTGGA ACCCGTCGAC
    AGTTTTGTAC CAGAAGTTGA TCCCAAGGCA ACTGCATATG ATCGACTTGA TCCTGGACAG
    ATAATGTACG CTGCCACCGC TCACGACCTC GACCTCGCCA AAACTGTACA GCAAGTCAAG
    AAAGGCGATA CTATCAAAGA TCCTGATTCA CGTGCTCTTA TCGCTGTTTC TGAACCAGAA
    GCTCGTAAGC CAGGAGGCAC TGAAGAAACG GATGGTGATG AAGATGAGGA TAATAAGGAT
    TCGAAAGTGG AAAGTACTGC TAAATTCATT CAAAAAATGA TCGCTTCTGC CTTGGACTTG
    TGTAGTGTAA CACTAAACAA ACTGTGCAGA GAACACCGAT ATGTTGGATT TGTTTTGAGC
    AAGGAAAAAC AAAAGTTGAA GTCAGGACAC TCGGAATCTC TTTCAAATAC ATCTAGAAAG
    CTGACGGATA TTCGATCTGC AGTTGATCTA CCAAGTCTTC AACTTGTGCA ATCGGCGAAT
    GATGTTGAAA AGATGGAGAC AGCCGTCAGC GTGGATTGGC AACAAAAATC ATCAGCAACG
    AGACTATTGA ATGCAGTTGT GAATTGTATT GGAGCACACA CTGATATTCT CTGCTACTTC
    TTCGCAATTA TGACACAAGT GATGACTGGT GGACTTATTA CTCTTCCACT TCCTCTGATG
    TCACTATTCT GGGGAAATCT ATCGAATCCT CGTCCATCCA AGTTCTTCTG GGTCACAATG
    ATCACCTACA CTGAGTGTGT CATTGTTATC AAGTTTGTTT GCCAATTTGC ATTCATGCCT
    TACAATTCTA TCACGTGGCG AACAGAGCAT CAAATGGACC CGATGTCATT GGATAAGTTA
    TTCGGAGTAT CACAGAGAGA TAGTTTTGCA CTTTGGGACA TTGTTTTGCT CTTCTCACTC
    TTCTTCCATC GGTATATGCT CAGAAAGTTA GGGTTATGGA AGGATGCCAA TTTGACGGAC
    ACGTTCACTT TGAAGGAAGA GCCTCGATCG GCGTCAGGAT CGGACACAGG ATCACCCAAG
    AAAATTGCTC AAGAGCCAAA GGTTGTTGTA ACTCAGTCAG ATACCCTTGA AGGTACAAGT
    GGAGGAGAGA TAGTCATACC ATCTGATCCA AATGCAGTTT CCAATATGGA AGAATTAGAC
    TGCGAACCTC CGATTCCAGA GAAGCAAAGT GGACCAATTG GAAGATTCAT TCATCAATTA
    TTCCATCCAA AATTCCGTTA CATTCGTGAT TTGTATCCAA TAATGTTTGG AATCGATGTG
    ATATGCTTCC TCATCATGAC ATTTGGATAC TCGGCATTTG GTGAAGGAGG TTCTGGAAAT
    GTTTTAGATG ATGTGAAGGC TTCTCGTATT CCGGTAACAC TTGTTGTTAT GCTCGTTGGA
    ATGACTCTGG CAATTATCAT TGATCGAGCA CTCTACCTCA GAAAATCAGT TGTCGGAAAG
    CTAATCTATC AAGTCTTAAT GATTGCTTTC CTTCACATTT GGGTATTCCT TGTTCTTCCA
    AATATGACTC GTCGTTCTGC GATATCGAAT CACGTTGCTC AAGCATTGTA CGTCATCAAA
    TCGTGCTATT TCCTTGTGTC TGCTTGGCAG ATCCGAAATG GTTATCCTGA ACTTTGTATT
    GGAAATCTAT TAACACATTC ATACGGAATG ACCAATATGA TTGCATTCAA AGTATTCATG
    AATATTCCAT TCCTCTTCGA GCTTCGTACA GCTATTGATT GGACATGGAC AGATACTTCA
    ATGCCTCTTT TCGATTTCTT CAATATGGAA AATTTCTATG CTCATATATT CAATATCAAG
    TGTGCCCGCC AGTTCGAAGC TGCCTATCCA GCTCCAAGAG GGATTCCCAA AGGAAAGTTG
    GTCAAGTATA TGATGGGATT CCCAATTATC ATTGGAGTCG TTATCTTCAT CTTTTCTCCT
    CTATTGCTCT GGTCGCTACT CAATCAAATC GGAACTATCA GTATGCCAGA AAAAGTTACG
    TTGAGGATTT CGATTGAAGG ATATCCACCA CTTTACGAGA TGGAGGCACA AGGAAGTAAT
    CACGATAACG CTGAACTTGG AATGATAAAA CCTGATCAAT TGGCATCTTT GAATCAAGCA
    TTGACGGATA GTTACACGAC ACGAGACACT AATTCTATTT TAAGATCAAG AATGTCTGTA
    TCATATCTGA AAGGATACAC ATATGAGGAT ATTCTGATTG TGCGATTCCG TCCAGAATCT
    GAAATTTATT GGCCGATTTC ACAGGATTCG AGAAATGCGA TGATCGATAA ATTGAGCCGA
    AACACGTCAG TCAATTTTGA AGTATCTCTG GAATTCACTC GGCCATATGA TCCAAATGAA
    AACGCTGCTT TAAAACATTC GAAATCGTGG CTTGTTCCAA TCTCCCTGGA TATGACGATT
    CGAGCAAAAA TTCAAAGTGC TCTCCGAGGA GATCCAGGTC ATCCGATTCT CATTCCACAG
    TCAATTCCTG CGTTTATTCA AGTTCCAAAT CAAGGAGAAT TGACCCTTCC AACATCAATC
    GGAAATACTA TTATCAATGA CGGAAATCCA CGGATTAATA CGACCGGGAT GGAAAAATCA
    GACGAAGCTC GGGCTTGGTT CGACTCGTTA ACCTTGAATC TCGAACAAGG AAAATCGCAA
    AACGAGAAAA TGTGGATCGC CACATCCGAA CATCCAGGAG ACCAAAATGC GAAACTCTGG
    ATAAAGACTG CAAATACGAC GTATTCGGGA AGGCCGTACC TTCAAGTTGT CGGATTTATT
    GATAGAGCAT TCCCATCATT TTTGGCGAAA GTTTTCAAAG GAGGAGTCAT TGCAGTGTAT
    CTTTCAGTTA TCCTCGTCGT TGGAAGAGGT CTGGTTCGTG GAATCTTCAC CACATCTCCA
    AGCACTGTCA TGTTCACAGA ATTACCGAAT GCGGATCATC TTCTCAAAAT TTGTCTCGAC
    ATCTATTTGG TTCGAGAAGC GAAGGACTTC ATGTTGGAGC AGGATCTCTT CGCCAAATTA
    ATATTCCTGT TCCGATCACC AGCGACACTC ATCGAATGGA CACGAATGAG CAAAAAGAAG
    CAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343008
    Clone ID Related Accession (Same CDS sequence) NM_001307051.1 , NM_001307051.2
    Accession Version NM_001307051.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7329bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACTACAGCAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGTTTCAGGA TCCAAAAAGT
    CGGAAGTTCG CGGCATTTGT GGAATATCTG TCGAATAAAG TGTCTGTCTA CTTCATATTT
    GTAGTATCAG TGGTACTTTT GGTGGTCTCT ACGTGTTTTG CTCCGAATTT CTACAATATT
    CTATTCTTCG CACTATGGGC ATTGAACTTG ATTTATCTCA AGTTCTCATT CCGTCTGTAT
    CGTGGATTGG CATATGCATT CTGGCTAACT CTCACGTTCT ACACTTCAAT TGTTATTATC
    GCCCTCTACA TTTATCAGTT CCCTGGAGTA TCTCAATGGA TTATTCGAAA TACTAGTTTG
    TCGCAGGAGT GGCTGAATGC AATTGGACTT GTCGATTTTA GAGCTATCGG TGAATCGGGA
    GCCCTATTTC TACAATTACT CGCCCCAATT GCCTTGTTCG TCGTCACAAT GCTCCAACTC
    AAGTTTTTCC ATGGACCATG GAGTCGAGCA ACGTCACCAA GAAGAGCAGA AAATGATCCA
    CCTACAAGTA CGACGGAGGC TGCGGCGGTA GCAAGCACAT CTGGAACACA AGGAAGAGCA
    CATGCTGCAG GCGATACCCT TGTCAAGAAG CTGCACAAAT TGGCAAATCA GACAATCGAA
    CTGTTATGGA GGTTTTTCGA AGTTCACATT TCGAAAATTG TCTTTGTCAT CATTGCGATT
    TTTATTGCCA ACAATATCAA TGCTCTATAC ATTCCACTTG TTATTCTCCT CTCATTGGCT
    ATATGTCTAC CATCAGCTGC AGATGGGATA TTCTCTTTAT TTATGTGTGC CTACTTATTC
    CTCGTGGCAC TGAGCAAGAT GATCTACCAA CTGGATATTG TTCCCGAGTT ATCACAAATC
    GACCGAGGAG TTGGAGCAGA TAACTGTTCA CATGGAAATA TTTCAATGCC TGAATGGTTT
    GGATTGAAGA AGGAAGTTGA AGGAACTGAA CCAATTTATA TGCTCTTTGG AGTCATTGTT
    TCGATTATTG CATTGGCTTT TCAATCAATT GTTATATATC GACAAAGACA TTATCGAGCT
    TCACTGGGAC TTCCTGAATC GATGCGAGCT AAAGTGTTCC CGGACTTCCA TCATTCTCAT
    TTTGATAGAT CTCTCAAAAA TGCCATCCAA TTCTTGATAG ACTATGGTTT CTATAAGTTT
    GGACTTGAAA TCACGATGAT CGCCATTGGA ATTGACATTT TCAATCGAAT GGATGCATTG
    GCAGCGATTC AATGTTTCTG GCTTGTCCTG TTTGCGCTCA ATAAAAGAGT CTTTGTTCGA
    AGAATATGGG TATTCTATGT GATCTATATG GCGATTCTGT ATCCACTTCA GTTCTTTTCA
    TATGTTGGAT TACCACCTGA CAGTTGTATT GAGTATCCAT GGTCGTATTG GATTCCATCG
    TATAGTGATG ATGCGAGATT CAATCTGTCG TATCTTCTGA ACTTGTCAAT ATACGGTGTA
    AACTGGCCAT CAGCATATCT CATTGGCGAT TTCTTCGTAC TCCTCTTGGC ATCATGCCAA
    TTAGCAGTTT TCCGGCGAGA AGGAGAAGAT AATGATTCAA TTTACAATGA TGGAAACTTT
    GTGATTAAGC CAGAAAATCC ACAATATGAC TTTATTGACA CCAAAAAGAG TTACGTTGAT
    TATTTCAAAT CATTCGTTTT CCATTATGGA CACTGGATTA CATTGATGTC TACACTAGCA
    GCAGGAATTG CTGGAACGTC ACTTTTTGCT TTGGGATACA TTATATTCAC TCTGACAATG
    CTGTGGTCTG GTAACAATTT GTATGTGATG AACTCAACTC TACGATCATT TGAACATACT
    TTGAAACGAT GGAATGCACT TTTGGGATAC ACATTGTTCA CGATTACAAT GAAAGTTTGC
    CTTCAAATAT TTGGATGTGT ATTCCTGTCG TGGTTCGATC AATCTGGTGG ATGGGGAAAG
    ACTCTGTGCA TCGTTCGTCA ACTCTTCAGT ATCACATGTG TTAATAACGA ATGTCATGTG
    CTCAAAGAGC TGGAGGATTT CTCGAAGGCG TGTGCTGTTG AGACGAAAGA AGGAAATATT
    GGATTTGATG TGATTGCGCT ATCCTTCCTC GTCTTCCAAA TCCGAATTTT CCATTCTTGG
    TATTTCCAAC ACTGCATGGT TGAATATCGA AGTGAGGTTA TTCTGGCCAA TAGAGGTGCC
    GTGCTGAAGA ATCAACTGAT TGAAAAGGAA ATGAAGGAAC AAAACGAGCA GCAAAAAGCC
    AAGTTCAATG ATATCAGAAG AAGAACTGAA GCTATTCGTG AAAGATATCA AAAACAAATC
    GAACGAGGAG CTGCTGAACG TGACTTCGAG CCGGTTACTT ATGGGCACGC CAAACGAGCG
    GGAGACTACT ACATGTTCAA ATACGATCCT GAAAATGATG ATCTTGTGGA ACCCGTCGAC
    AGTTTTGTAC CAGAAGTTGA TCCCAAGGCA ACTGCATATG ATCGACTTGA TCCTGGACAG
    ATAATGTACG CTGCCACCGC TCACGACCTC GACCTCGCCA AAACTGTACA GCAAGTCAAG
    AAAGGCGATA CTATCAAAGA TCCTGATTCA CGTGCTCTTA TCGCTGTTTC TGAACCAGAA
    GCTCGTAAGC CAGGAGGCAC TGAAGAAACG GATGGTGATG AAGATGAGGA TAATAAGGAT
    TCGAAAGTGG AAAGTACTGC TAAATTCATT CAAAAAATGA TCGCTTCTGC CTTGGACTTG
    TGTAGTGTAA CACTAAACAA ACTGTGCAGA GAACACCGAT ATGTTGGATT TGTTTTGAGC
    AAGGAAAAAC AAAAGTTGAA GTCAGGACAC TCGGAATCTC TTTCAAATAC ATCTAGAAAG
    CTGACGGATA TTCGATCTGC AGTTGATCTA CCAAGTCTTC AACTTGTGCA ATCGGCGAAT
    GATGTTGAAA AGATGGAGAC AGCCGTCAGC GTGGATTGGC AACAAAAATC ATCAGCAACG
    AGACTATTGA ATGCAGTTGT GAATTGTATT GGAGCACACA CTGATATTCT CTGCTACTTC
    TTCGCAATTA TGACACAAGT GATGACTGGT GGACTTATTA CTCTTCCACT TCCTCTGATG
    TCACTATTCT GGGGAAATCT ATCGAATCCT CGTCCATCCA AGTTCTTCTG GGTCACAATG
    ATCACCTACA CTGAGTGTGT CATTGTTATC AAGTTTGTTT GCCAATTTGC ATTCATGCCT
    TACAATTCTA TCACGTGGCG AACAGAGCAT CAAATGGACC CGATGTCATT GGATAAGTTA
    TTCGGAGTAT CACAGAGAGA TAGTTTTGCA CTTTGGGACA TTGTTTTGCT CTTCTCACTC
    TTCTTCCATC GGTATATGCT CAGAAAGTTA GGGTTATGGA AGGATGCCAA TTTGACGGAC
    ACGTTCACTT TGAAGGAAGA GCCTCGATCG GCGTCAGGAT CGGACACAGG ATCACCCAAG
    AAAATTGCTC AAGAGCCAAA GGTTGTTGTA ACTCAGTCAG ATACCCTTGA AGGTACAAGT
    GGAGGAGAGA TAGTCATACC ATCTGATCCA AATGCAGTTT CCAATATGGA AGAATTAGAC
    TGCGAACCTC CGATTCCAGA GAAGCAAAGT GGACCAATTG GAAGATTCAT TCATCAATTA
    TTCCATCCAA AATTCCGTTA CATTCGTGAT TTGTATCCAA TAATGTTTGG AATCGATGTG
    ATATGCTTCC TCATCATGAC ATTTGGATAC TCGGCATTTG GTGAAGGAGG TTCTGGAAAT
    GTTTTAGATG ATGTGAAGGC TTCTCGTATT CCGGTAACAC TTGTTGTTAT GCTCGTTGGA
    ATGACTCTGG CAATTATCAT TGATCGAGCA CTCTACCTCA GAAAATCAGT TGTCGGAAAG
    CTAATCTATC AAGTCTTAAT GATTGCTTTC CTTCACATTT GGGTATTCCT TGTTCTTCCA
    AATATGACTC GTCGTTCTGC GATATCGAAT CACGTTGCTC AAGCATTGTA CGTCATCAAA
    TCGTGCTATT TCCTTGTGTC TGCTTGGCAG ATCCGAAATG GTTATCCTGA ACTTTGTATT
    GGAAATCTAT TAACACATTC ATACGGAATG ACCAATATGA TTGCATTCAA AGTATTCATG
    AATATTCCAT TCCTCTTCGA GCTTCGTACA GCTATTGATT GGACATGGAC AGATACTTCA
    ATGCCTCTTT TCGATTTCTT CAATATGGAA AATTTCTATG CTCATATATT CAATATCAAG
    TGTGCCCGCC AGTTCGAAGC TGCCTATCCA GCTCCAAGAG GGATTCCCAA AGGAAAGTTG
    GTCAAGTATA TGATGGGATT CCCAATTATC ATTGGAGTCG TTATCTTCAT CTTTTCTCCT
    CTATTGCTCT GGTCGCTACT CAATCAAATC GGAACTATCA GTATGCCAGA AAAAGTTACG
    TTGAGGATTT CGATTGAAGG ATATCCACCA CTTTACGAGA TGGAGGCACA AGGAAGTAAT
    CACGATAACG CTGAACTTGG AATGATAAAA CCTGATCAAT TGGCATCTTT GAATCAAGCA
    TTGACGGATA GTTACACGAC ACGAGACACT AATTCTATTT TAAGATCAAG AATGTCTGTA
    TCATATCTGA AAGGATACAC ATATGAGGAT ATTCTGATTG TGCGATTCCG TCCAGAATCT
    GAAATTTATT GGCCGATTTC ACAGGATTCG AGAAATGCGA TGATCGATAA ATTGAGCCGA
    AACACGTCAG TCAATTTTGA AGTATCTCTG GAATTCACTC GGCCATATGA TCCAAATGAA
    AACGCTGCTT TAAAACATTC GAAATCGTGG CTTGTTCCAA TCTCCCTGGA TATGACGATT
    CGAGCAAAAA TTCAAAGTGC TCTCCGAGGA GATCCAGGTC ATCCGATTCT CATTCCACAG
    TCAATTCCTG CGTTTATTCA AGTTCCAAAT CAAGGAGAAT TGACCCTTCC AACATCAATC
    GGAAATACTA TTATCAATGA CGGAAATCCA CGGATTAATA CGACCGGGAT GGAAAAATCA
    GACGAAGCTC GGGCTTGGTT CGACTCGTTA ACCTTGAATC TCGAACAAGG AAAATCGCAA
    AACGAGAAAA TGTGGATCGC CACATCCGAA CATCCAGGAG ACCAAAATGC GAAACTCTGG
    ATAAAGACTG CAAATACGAC GTATTCGGGA AGGCCGTACC TTCAAGTTGT CGGATTTATT
    GATAGAGCAT TCCCATCATT TTTGGCGAAA GTTTTCAAAG GAGGAGTCAT TGCAGTGTAT
    CTTTCAGTTA TCCTCGTCGT TGGAAGAGGT CTGGTTCGTG GAATCTTCAC CACATCTCCA
    AGCACTGTCA TGTTCACAGA ATTACCGAAT GCGGATCATC TTCTCAAAAT TTGTCTCGAC
    ATCTATTTGG TTCGAGAAGC GAAGGACTTC ATGTTGGAGC AGGATCTCTT CGCCAAATTA
    ATATTCCTGT TCCGATCACC AGCGACACTC ATCGAATGGA CACGAATGAG CAAAAAGAAG
    CAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293980.1
    CDS1..7329
    Translation

    Target ORF information:

    RefSeq Version NM_001307051.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307051.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACTACAGCAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGTTTCAGGA TCCAAAAAGT
    CGGAAGTTCG CGGCATTTGT GGAATATCTG TCGAATAAAG TGTCTGTCTA CTTCATATTT
    GTAGTATCAG TGGTACTTTT GGTGGTCTCT ACGTGTTTTG CTCCGAATTT CTACAATATT
    CTATTCTTCG CACTATGGGC ATTGAACTTG ATTTATCTCA AGTTCTCATT CCGTCTGTAT
    CGTGGATTGG CATATGCATT CTGGCTAACT CTCACGTTCT ACACTTCAAT TGTTATTATC
    GCCCTCTACA TTTATCAGTT CCCTGGAGTA TCTCAATGGA TTATTCGAAA TACTAGTTTG
    TCGCAGGAGT GGCTGAATGC AATTGGACTT GTCGATTTTA GAGCTATCGG TGAATCGGGA
    GCCCTATTTC TACAATTACT CGCCCCAATT GCCTTGTTCG TCGTCACAAT GCTCCAACTC
    AAGTTTTTCC ATGGACCATG GAGTCGAGCA ACGTCACCAA GAAGAGCAGA AAATGATCCA
    CCTACAAGTA CGACGGAGGC TGCGGCGGTA GCAAGCACAT CTGGAACACA AGGAAGAGCA
    CATGCTGCAG GCGATACCCT TGTCAAGAAG CTGCACAAAT TGGCAAATCA GACAATCGAA
    CTGTTATGGA GGTTTTTCGA AGTTCACATT TCGAAAATTG TCTTTGTCAT CATTGCGATT
    TTTATTGCCA ACAATATCAA TGCTCTATAC ATTCCACTTG TTATTCTCCT CTCATTGGCT
    ATATGTCTAC CATCAGCTGC AGATGGGATA TTCTCTTTAT TTATGTGTGC CTACTTATTC
    CTCGTGGCAC TGAGCAAGAT GATCTACCAA CTGGATATTG TTCCCGAGTT ATCACAAATC
    GACCGAGGAG TTGGAGCAGA TAACTGTTCA CATGGAAATA TTTCAATGCC TGAATGGTTT
    GGATTGAAGA AGGAAGTTGA AGGAACTGAA CCAATTTATA TGCTCTTTGG AGTCATTGTT
    TCGATTATTG CATTGGCTTT TCAATCAATT GTTATATATC GACAAAGACA TTATCGAGCT
    TCACTGGGAC TTCCTGAATC GATGCGAGCT AAAGTGTTCC CGGACTTCCA TCATTCTCAT
    TTTGATAGAT CTCTCAAAAA TGCCATCCAA TTCTTGATAG ACTATGGTTT CTATAAGTTT
    GGACTTGAAA TCACGATGAT CGCCATTGGA ATTGACATTT TCAATCGAAT GGATGCATTG
    GCAGCGATTC AATGTTTCTG GCTTGTCCTG TTTGCGCTCA ATAAAAGAGT CTTTGTTCGA
    AGAATATGGG TATTCTATGT GATCTATATG GCGATTCTGT ATCCACTTCA GTTCTTTTCA
    TATGTTGGAT TACCACCTGA CAGTTGTATT GAGTATCCAT GGTCGTATTG GATTCCATCG
    TATAGTGATG ATGCGAGATT CAATCTGTCG TATCTTCTGA ACTTGTCAAT ATACGGTGTA
    AACTGGCCAT CAGCATATCT CATTGGCGAT TTCTTCGTAC TCCTCTTGGC ATCATGCCAA
    TTAGCAGTTT TCCGGCGAGA AGGAGAAGAT AATGATTCAA TTTACAATGA TGGAAACTTT
    GTGATTAAGC CAGAAAATCC ACAATATGAC TTTATTGACA CCAAAAAGAG TTACGTTGAT
    TATTTCAAAT CATTCGTTTT CCATTATGGA CACTGGATTA CATTGATGTC TACACTAGCA
    GCAGGAATTG CTGGAACGTC ACTTTTTGCT TTGGGATACA TTATATTCAC TCTGACAATG
    CTGTGGTCTG GTAACAATTT GTATGTGATG AACTCAACTC TACGATCATT TGAACATACT
    TTGAAACGAT GGAATGCACT TTTGGGATAC ACATTGTTCA CGATTACAAT GAAAGTTTGC
    CTTCAAATAT TTGGATGTGT ATTCCTGTCG TGGTTCGATC AATCTGGTGG ATGGGGAAAG
    ACTCTGTGCA TCGTTCGTCA ACTCTTCAGT ATCACATGTG TTAATAACGA ATGTCATGTG
    CTCAAAGAGC TGGAGGATTT CTCGAAGGCG TGTGCTGTTG AGACGAAAGA AGGAAATATT
    GGATTTGATG TGATTGCGCT ATCCTTCCTC GTCTTCCAAA TCCGAATTTT CCATTCTTGG
    TATTTCCAAC ACTGCATGGT TGAATATCGA AGTGAGGTTA TTCTGGCCAA TAGAGGTGCC
    GTGCTGAAGA ATCAACTGAT TGAAAAGGAA ATGAAGGAAC AAAACGAGCA GCAAAAAGCC
    AAGTTCAATG ATATCAGAAG AAGAACTGAA GCTATTCGTG AAAGATATCA AAAACAAATC
    GAACGAGGAG CTGCTGAACG TGACTTCGAG CCGGTTACTT ATGGGCACGC CAAACGAGCG
    GGAGACTACT ACATGTTCAA ATACGATCCT GAAAATGATG ATCTTGTGGA ACCCGTCGAC
    AGTTTTGTAC CAGAAGTTGA TCCCAAGGCA ACTGCATATG ATCGACTTGA TCCTGGACAG
    ATAATGTACG CTGCCACCGC TCACGACCTC GACCTCGCCA AAACTGTACA GCAAGTCAAG
    AAAGGCGATA CTATCAAAGA TCCTGATTCA CGTGCTCTTA TCGCTGTTTC TGAACCAGAA
    GCTCGTAAGC CAGGAGGCAC TGAAGAAACG GATGGTGATG AAGATGAGGA TAATAAGGAT
    TCGAAAGTGG AAAGTACTGC TAAATTCATT CAAAAAATGA TCGCTTCTGC CTTGGACTTG
    TGTAGTGTAA CACTAAACAA ACTGTGCAGA GAACACCGAT ATGTTGGATT TGTTTTGAGC
    AAGGAAAAAC AAAAGTTGAA GTCAGGACAC TCGGAATCTC TTTCAAATAC ATCTAGAAAG
    CTGACGGATA TTCGATCTGC AGTTGATCTA CCAAGTCTTC AACTTGTGCA ATCGGCGAAT
    GATGTTGAAA AGATGGAGAC AGCCGTCAGC GTGGATTGGC AACAAAAATC ATCAGCAACG
    AGACTATTGA ATGCAGTTGT GAATTGTATT GGAGCACACA CTGATATTCT CTGCTACTTC
    TTCGCAATTA TGACACAAGT GATGACTGGT GGACTTATTA CTCTTCCACT TCCTCTGATG
    TCACTATTCT GGGGAAATCT ATCGAATCCT CGTCCATCCA AGTTCTTCTG GGTCACAATG
    ATCACCTACA CTGAGTGTGT CATTGTTATC AAGTTTGTTT GCCAATTTGC ATTCATGCCT
    TACAATTCTA TCACGTGGCG AACAGAGCAT CAAATGGACC CGATGTCATT GGATAAGTTA
    TTCGGAGTAT CACAGAGAGA TAGTTTTGCA CTTTGGGACA TTGTTTTGCT CTTCTCACTC
    TTCTTCCATC GGTATATGCT CAGAAAGTTA GGGTTATGGA AGGATGCCAA TTTGACGGAC
    ACGTTCACTT TGAAGGAAGA GCCTCGATCG GCGTCAGGAT CGGACACAGG ATCACCCAAG
    AAAATTGCTC AAGAGCCAAA GGTTGTTGTA ACTCAGTCAG ATACCCTTGA AGGTACAAGT
    GGAGGAGAGA TAGTCATACC ATCTGATCCA AATGCAGTTT CCAATATGGA AGAATTAGAC
    TGCGAACCTC CGATTCCAGA GAAGCAAAGT GGACCAATTG GAAGATTCAT TCATCAATTA
    TTCCATCCAA AATTCCGTTA CATTCGTGAT TTGTATCCAA TAATGTTTGG AATCGATGTG
    ATATGCTTCC TCATCATGAC ATTTGGATAC TCGGCATTTG GTGAAGGAGG TTCTGGAAAT
    GTTTTAGATG ATGTGAAGGC TTCTCGTATT CCGGTAACAC TTGTTGTTAT GCTCGTTGGA
    ATGACTCTGG CAATTATCAT TGATCGAGCA CTCTACCTCA GAAAATCAGT TGTCGGAAAG
    CTAATCTATC AAGTCTTAAT GATTGCTTTC CTTCACATTT GGGTATTCCT TGTTCTTCCA
    AATATGACTC GTCGTTCTGC GATATCGAAT CACGTTGCTC AAGCATTGTA CGTCATCAAA
    TCGTGCTATT TCCTTGTGTC TGCTTGGCAG ATCCGAAATG GTTATCCTGA ACTTTGTATT
    GGAAATCTAT TAACACATTC ATACGGAATG ACCAATATGA TTGCATTCAA AGTATTCATG
    AATATTCCAT TCCTCTTCGA GCTTCGTACA GCTATTGATT GGACATGGAC AGATACTTCA
    ATGCCTCTTT TCGATTTCTT CAATATGGAA AATTTCTATG CTCATATATT CAATATCAAG
    TGTGCCCGCC AGTTCGAAGC TGCCTATCCA GCTCCAAGAG GGATTCCCAA AGGAAAGTTG
    GTCAAGTATA TGATGGGATT CCCAATTATC ATTGGAGTCG TTATCTTCAT CTTTTCTCCT
    CTATTGCTCT GGTCGCTACT CAATCAAATC GGAACTATCA GTATGCCAGA AAAAGTTACG
    TTGAGGATTT CGATTGAAGG ATATCCACCA CTTTACGAGA TGGAGGCACA AGGAAGTAAT
    CACGATAACG CTGAACTTGG AATGATAAAA CCTGATCAAT TGGCATCTTT GAATCAAGCA
    TTGACGGATA GTTACACGAC ACGAGACACT AATTCTATTT TAAGATCAAG AATGTCTGTA
    TCATATCTGA AAGGATACAC ATATGAGGAT ATTCTGATTG TGCGATTCCG TCCAGAATCT
    GAAATTTATT GGCCGATTTC ACAGGATTCG AGAAATGCGA TGATCGATAA ATTGAGCCGA
    AACACGTCAG TCAATTTTGA AGTATCTCTG GAATTCACTC GGCCATATGA TCCAAATGAA
    AACGCTGCTT TAAAACATTC GAAATCGTGG CTTGTTCCAA TCTCCCTGGA TATGACGATT
    CGAGCAAAAA TTCAAAGTGC TCTCCGAGGA GATCCAGGTC ATCCGATTCT CATTCCACAG
    TCAATTCCTG CGTTTATTCA AGTTCCAAAT CAAGGAGAAT TGACCCTTCC AACATCAATC
    GGAAATACTA TTATCAATGA CGGAAATCCA CGGATTAATA CGACCGGGAT GGAAAAATCA
    GACGAAGCTC GGGCTTGGTT CGACTCGTTA ACCTTGAATC TCGAACAAGG AAAATCGCAA
    AACGAGAAAA TGTGGATCGC CACATCCGAA CATCCAGGAG ACCAAAATGC GAAACTCTGG
    ATAAAGACTG CAAATACGAC GTATTCGGGA AGGCCGTACC TTCAAGTTGT CGGATTTATT
    GATAGAGCAT TCCCATCATT TTTGGCGAAA GTTTTCAAAG GAGGAGTCAT TGCAGTGTAT
    CTTTCAGTTA TCCTCGTCGT TGGAAGAGGT CTGGTTCGTG GAATCTTCAC CACATCTCCA
    AGCACTGTCA TGTTCACAGA ATTACCGAAT GCGGATCATC TTCTCAAAAT TTGTCTCGAC
    ATCTATTTGG TTCGAGAAGC GAAGGACTTC ATGTTGGAGC AGGATCTCTT CGCCAAATTA
    ATATTCCTGT TCCGATCACC AGCGACACTC ATCGAATGGA CACGAATGAG CAAAAAGAAG
    CAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343008
    Clone ID Related Accession (Same CDS sequence) NM_001307051.1 , NM_001307051.2
    Accession Version NM_001307051.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7329bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307051.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACTACAGCAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGTTTCAGGA TCCAAAAAGT
    CGGAAGTTCG CGGCATTTGT GGAATATCTG TCGAATAAAG TGTCTGTCTA CTTCATATTT
    GTAGTATCAG TGGTACTTTT GGTGGTCTCT ACGTGTTTTG CTCCGAATTT CTACAATATT
    CTATTCTTCG CACTATGGGC ATTGAACTTG ATTTATCTCA AGTTCTCATT CCGTCTGTAT
    CGTGGATTGG CATATGCATT CTGGCTAACT CTCACGTTCT ACACTTCAAT TGTTATTATC
    GCCCTCTACA TTTATCAGTT CCCTGGAGTA TCTCAATGGA TTATTCGAAA TACTAGTTTG
    TCGCAGGAGT GGCTGAATGC AATTGGACTT GTCGATTTTA GAGCTATCGG TGAATCGGGA
    GCCCTATTTC TACAATTACT CGCCCCAATT GCCTTGTTCG TCGTCACAAT GCTCCAACTC
    AAGTTTTTCC ATGGACCATG GAGTCGAGCA ACGTCACCAA GAAGAGCAGA AAATGATCCA
    CCTACAAGTA CGACGGAGGC TGCGGCGGTA GCAAGCACAT CTGGAACACA AGGAAGAGCA
    CATGCTGCAG GCGATACCCT TGTCAAGAAG CTGCACAAAT TGGCAAATCA GACAATCGAA
    CTGTTATGGA GGTTTTTCGA AGTTCACATT TCGAAAATTG TCTTTGTCAT CATTGCGATT
    TTTATTGCCA ACAATATCAA TGCTCTATAC ATTCCACTTG TTATTCTCCT CTCATTGGCT
    ATATGTCTAC CATCAGCTGC AGATGGGATA TTCTCTTTAT TTATGTGTGC CTACTTATTC
    CTCGTGGCAC TGAGCAAGAT GATCTACCAA CTGGATATTG TTCCCGAGTT ATCACAAATC
    GACCGAGGAG TTGGAGCAGA TAACTGTTCA CATGGAAATA TTTCAATGCC TGAATGGTTT
    GGATTGAAGA AGGAAGTTGA AGGAACTGAA CCAATTTATA TGCTCTTTGG AGTCATTGTT
    TCGATTATTG CATTGGCTTT TCAATCAATT GTTATATATC GACAAAGACA TTATCGAGCT
    TCACTGGGAC TTCCTGAATC GATGCGAGCT AAAGTGTTCC CGGACTTCCA TCATTCTCAT
    TTTGATAGAT CTCTCAAAAA TGCCATCCAA TTCTTGATAG ACTATGGTTT CTATAAGTTT
    GGACTTGAAA TCACGATGAT CGCCATTGGA ATTGACATTT TCAATCGAAT GGATGCATTG
    GCAGCGATTC AATGTTTCTG GCTTGTCCTG TTTGCGCTCA ATAAAAGAGT CTTTGTTCGA
    AGAATATGGG TATTCTATGT GATCTATATG GCGATTCTGT ATCCACTTCA GTTCTTTTCA
    TATGTTGGAT TACCACCTGA CAGTTGTATT GAGTATCCAT GGTCGTATTG GATTCCATCG
    TATAGTGATG ATGCGAGATT CAATCTGTCG TATCTTCTGA ACTTGTCAAT ATACGGTGTA
    AACTGGCCAT CAGCATATCT CATTGGCGAT TTCTTCGTAC TCCTCTTGGC ATCATGCCAA
    TTAGCAGTTT TCCGGCGAGA AGGAGAAGAT AATGATTCAA TTTACAATGA TGGAAACTTT
    GTGATTAAGC CAGAAAATCC ACAATATGAC TTTATTGACA CCAAAAAGAG TTACGTTGAT
    TATTTCAAAT CATTCGTTTT CCATTATGGA CACTGGATTA CATTGATGTC TACACTAGCA
    GCAGGAATTG CTGGAACGTC ACTTTTTGCT TTGGGATACA TTATATTCAC TCTGACAATG
    CTGTGGTCTG GTAACAATTT GTATGTGATG AACTCAACTC TACGATCATT TGAACATACT
    TTGAAACGAT GGAATGCACT TTTGGGATAC ACATTGTTCA CGATTACAAT GAAAGTTTGC
    CTTCAAATAT TTGGATGTGT ATTCCTGTCG TGGTTCGATC AATCTGGTGG ATGGGGAAAG
    ACTCTGTGCA TCGTTCGTCA ACTCTTCAGT ATCACATGTG TTAATAACGA ATGTCATGTG
    CTCAAAGAGC TGGAGGATTT CTCGAAGGCG TGTGCTGTTG AGACGAAAGA AGGAAATATT
    GGATTTGATG TGATTGCGCT ATCCTTCCTC GTCTTCCAAA TCCGAATTTT CCATTCTTGG
    TATTTCCAAC ACTGCATGGT TGAATATCGA AGTGAGGTTA TTCTGGCCAA TAGAGGTGCC
    GTGCTGAAGA ATCAACTGAT TGAAAAGGAA ATGAAGGAAC AAAACGAGCA GCAAAAAGCC
    AAGTTCAATG ATATCAGAAG AAGAACTGAA GCTATTCGTG AAAGATATCA AAAACAAATC
    GAACGAGGAG CTGCTGAACG TGACTTCGAG CCGGTTACTT ATGGGCACGC CAAACGAGCG
    GGAGACTACT ACATGTTCAA ATACGATCCT GAAAATGATG ATCTTGTGGA ACCCGTCGAC
    AGTTTTGTAC CAGAAGTTGA TCCCAAGGCA ACTGCATATG ATCGACTTGA TCCTGGACAG
    ATAATGTACG CTGCCACCGC TCACGACCTC GACCTCGCCA AAACTGTACA GCAAGTCAAG
    AAAGGCGATA CTATCAAAGA TCCTGATTCA CGTGCTCTTA TCGCTGTTTC TGAACCAGAA
    GCTCGTAAGC CAGGAGGCAC TGAAGAAACG GATGGTGATG AAGATGAGGA TAATAAGGAT
    TCGAAAGTGG AAAGTACTGC TAAATTCATT CAAAAAATGA TCGCTTCTGC CTTGGACTTG
    TGTAGTGTAA CACTAAACAA ACTGTGCAGA GAACACCGAT ATGTTGGATT TGTTTTGAGC
    AAGGAAAAAC AAAAGTTGAA GTCAGGACAC TCGGAATCTC TTTCAAATAC ATCTAGAAAG
    CTGACGGATA TTCGATCTGC AGTTGATCTA CCAAGTCTTC AACTTGTGCA ATCGGCGAAT
    GATGTTGAAA AGATGGAGAC AGCCGTCAGC GTGGATTGGC AACAAAAATC ATCAGCAACG
    AGACTATTGA ATGCAGTTGT GAATTGTATT GGAGCACACA CTGATATTCT CTGCTACTTC
    TTCGCAATTA TGACACAAGT GATGACTGGT GGACTTATTA CTCTTCCACT TCCTCTGATG
    TCACTATTCT GGGGAAATCT ATCGAATCCT CGTCCATCCA AGTTCTTCTG GGTCACAATG
    ATCACCTACA CTGAGTGTGT CATTGTTATC AAGTTTGTTT GCCAATTTGC ATTCATGCCT
    TACAATTCTA TCACGTGGCG AACAGAGCAT CAAATGGACC CGATGTCATT GGATAAGTTA
    TTCGGAGTAT CACAGAGAGA TAGTTTTGCA CTTTGGGACA TTGTTTTGCT CTTCTCACTC
    TTCTTCCATC GGTATATGCT CAGAAAGTTA GGGTTATGGA AGGATGCCAA TTTGACGGAC
    ACGTTCACTT TGAAGGAAGA GCCTCGATCG GCGTCAGGAT CGGACACAGG ATCACCCAAG
    AAAATTGCTC AAGAGCCAAA GGTTGTTGTA ACTCAGTCAG ATACCCTTGA AGGTACAAGT
    GGAGGAGAGA TAGTCATACC ATCTGATCCA AATGCAGTTT CCAATATGGA AGAATTAGAC
    TGCGAACCTC CGATTCCAGA GAAGCAAAGT GGACCAATTG GAAGATTCAT TCATCAATTA
    TTCCATCCAA AATTCCGTTA CATTCGTGAT TTGTATCCAA TAATGTTTGG AATCGATGTG
    ATATGCTTCC TCATCATGAC ATTTGGATAC TCGGCATTTG GTGAAGGAGG TTCTGGAAAT
    GTTTTAGATG ATGTGAAGGC TTCTCGTATT CCGGTAACAC TTGTTGTTAT GCTCGTTGGA
    ATGACTCTGG CAATTATCAT TGATCGAGCA CTCTACCTCA GAAAATCAGT TGTCGGAAAG
    CTAATCTATC AAGTCTTAAT GATTGCTTTC CTTCACATTT GGGTATTCCT TGTTCTTCCA
    AATATGACTC GTCGTTCTGC GATATCGAAT CACGTTGCTC AAGCATTGTA CGTCATCAAA
    TCGTGCTATT TCCTTGTGTC TGCTTGGCAG ATCCGAAATG GTTATCCTGA ACTTTGTATT
    GGAAATCTAT TAACACATTC ATACGGAATG ACCAATATGA TTGCATTCAA AGTATTCATG
    AATATTCCAT TCCTCTTCGA GCTTCGTACA GCTATTGATT GGACATGGAC AGATACTTCA
    ATGCCTCTTT TCGATTTCTT CAATATGGAA AATTTCTATG CTCATATATT CAATATCAAG
    TGTGCCCGCC AGTTCGAAGC TGCCTATCCA GCTCCAAGAG GGATTCCCAA AGGAAAGTTG
    GTCAAGTATA TGATGGGATT CCCAATTATC ATTGGAGTCG TTATCTTCAT CTTTTCTCCT
    CTATTGCTCT GGTCGCTACT CAATCAAATC GGAACTATCA GTATGCCAGA AAAAGTTACG
    TTGAGGATTT CGATTGAAGG ATATCCACCA CTTTACGAGA TGGAGGCACA AGGAAGTAAT
    CACGATAACG CTGAACTTGG AATGATAAAA CCTGATCAAT TGGCATCTTT GAATCAAGCA
    TTGACGGATA GTTACACGAC ACGAGACACT AATTCTATTT TAAGATCAAG AATGTCTGTA
    TCATATCTGA AAGGATACAC ATATGAGGAT ATTCTGATTG TGCGATTCCG TCCAGAATCT
    GAAATTTATT GGCCGATTTC ACAGGATTCG AGAAATGCGA TGATCGATAA ATTGAGCCGA
    AACACGTCAG TCAATTTTGA AGTATCTCTG GAATTCACTC GGCCATATGA TCCAAATGAA
    AACGCTGCTT TAAAACATTC GAAATCGTGG CTTGTTCCAA TCTCCCTGGA TATGACGATT
    CGAGCAAAAA TTCAAAGTGC TCTCCGAGGA GATCCAGGTC ATCCGATTCT CATTCCACAG
    TCAATTCCTG CGTTTATTCA AGTTCCAAAT CAAGGAGAAT TGACCCTTCC AACATCAATC
    GGAAATACTA TTATCAATGA CGGAAATCCA CGGATTAATA CGACCGGGAT GGAAAAATCA
    GACGAAGCTC GGGCTTGGTT CGACTCGTTA ACCTTGAATC TCGAACAAGG AAAATCGCAA
    AACGAGAAAA TGTGGATCGC CACATCCGAA CATCCAGGAG ACCAAAATGC GAAACTCTGG
    ATAAAGACTG CAAATACGAC GTATTCGGGA AGGCCGTACC TTCAAGTTGT CGGATTTATT
    GATAGAGCAT TCCCATCATT TTTGGCGAAA GTTTTCAAAG GAGGAGTCAT TGCAGTGTAT
    CTTTCAGTTA TCCTCGTCGT TGGAAGAGGT CTGGTTCGTG GAATCTTCAC CACATCTCCA
    AGCACTGTCA TGTTCACAGA ATTACCGAAT GCGGATCATC TTCTCAAAAT TTGTCTCGAC
    ATCTATTTGG TTCGAGAAGC GAAGGACTTC ATGTTGGAGC AGGATCTCTT CGCCAAATTA
    ATATTCCTGT TCCGATCACC AGCGACACTC ATCGAATGGA CACGAATGAG CAAAAAGAAG
    CAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293980.1
    CDS57..7385
    Translation

    Target ORF information:

    RefSeq Version NM_001307051.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307051.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CGTTTTGTCT CCGCCGGCAC TGTCGAAACG
    AATGTGGACG ACGTGGGTCA GCTCATTTCA ATTTCTGAGA GCACAGCTTC TGCTCCATCT
    GGCAGAGGAC GTGGAAATAC TTTGCTTCTG TCCAATGCAT CCTCCTCTGC TAATGATGAT
    GAACAGGGTC GAGCTCGTTC GCGATCTCCG CTGCGAAATG GTGAAGAACA AGGCTCAATC
    CCACTACGGA AAGTTACTTC GCAAGTTGTT GATAGGAATA AGTTGAGCAA CATTTTTAAT
    ACTACAGCAC CCGGAGATAA GGAATCTGCT GCTTCGAAAG GAATGATTGC AGTCATGACA
    TTTGTCATTT TCCATTCGTA TTCAATCGCG CTGACTGCTA TGATGACATG GGCTCTCCTC
    TATCATTCGA TATTCGGTCT CATTCTCCTC ATCCTCACAT GTATCTTGTG GATCTTCCGT
    GATACGAGAA AGTCATCGTT TGCAATGGCC CCTATTATTT TGATGTATAT CGAGTTTCTA
    TTGATTCTTC AATATTTCCT CAGTATGGAT ATTCATGCAG AAATTGGCGA TCCAGCGTGG
    ATGAACTTTG TTGGAATCGA ATGGACTACT CTTCCAGTAC ATGCTGTCAT CATTCTCTGT
    GTCCAGACTC TTCTCACTCT CCCAGTGTTC TTGCTTCTAC GTTTGGCTCG CAGGGAGAAG
    TTTTACGAAT CGCTGAGTGA TTATGAACGA CAACGACGAA TCAACTCATA TGGAACATTT
    GGAGCATCAA AGACTGGAGC TGGGGGTGTT GCAGTGGCTA AGTTTCAGGA TCCAAAAAGT
    CGGAAGTTCG CGGCATTTGT GGAATATCTG TCGAATAAAG TGTCTGTCTA CTTCATATTT
    GTAGTATCAG TGGTACTTTT GGTGGTCTCT ACGTGTTTTG CTCCGAATTT CTACAATATT
    CTATTCTTCG CACTATGGGC ATTGAACTTG ATTTATCTCA AGTTCTCATT CCGTCTGTAT
    CGTGGATTGG CATATGCATT CTGGCTAACT CTCACGTTCT ACACTTCAAT TGTTATTATC
    GCCCTCTACA TTTATCAGTT CCCTGGAGTA TCTCAATGGA TTATTCGAAA TACTAGTTTG
    TCGCAGGAGT GGCTGAATGC AATTGGACTT GTCGATTTTA GAGCTATCGG TGAATCGGGA
    GCCCTATTTC TACAATTACT CGCCCCAATT GCCTTGTTCG TCGTCACAAT GCTCCAACTC
    AAGTTTTTCC ATGGACCATG GAGTCGAGCA ACGTCACCAA GAAGAGCAGA AAATGATCCA
    CCTACAAGTA CGACGGAGGC TGCGGCGGTA GCAAGCACAT CTGGAACACA AGGAAGAGCA
    CATGCTGCAG GCGATACCCT TGTCAAGAAG CTGCACAAAT TGGCAAATCA GACAATCGAA
    CTGTTATGGA GGTTTTTCGA AGTTCACATT TCGAAAATTG TCTTTGTCAT CATTGCGATT
    TTTATTGCCA ACAATATCAA TGCTCTATAC ATTCCACTTG TTATTCTCCT CTCATTGGCT
    ATATGTCTAC CATCAGCTGC AGATGGGATA TTCTCTTTAT TTATGTGTGC CTACTTATTC
    CTCGTGGCAC TGAGCAAGAT GATCTACCAA CTGGATATTG TTCCCGAGTT ATCACAAATC
    GACCGAGGAG TTGGAGCAGA TAACTGTTCA CATGGAAATA TTTCAATGCC TGAATGGTTT
    GGATTGAAGA AGGAAGTTGA AGGAACTGAA CCAATTTATA TGCTCTTTGG AGTCATTGTT
    TCGATTATTG CATTGGCTTT TCAATCAATT GTTATATATC GACAAAGACA TTATCGAGCT
    TCACTGGGAC TTCCTGAATC GATGCGAGCT AAAGTGTTCC CGGACTTCCA TCATTCTCAT
    TTTGATAGAT CTCTCAAAAA TGCCATCCAA TTCTTGATAG ACTATGGTTT CTATAAGTTT
    GGACTTGAAA TCACGATGAT CGCCATTGGA ATTGACATTT TCAATCGAAT GGATGCATTG
    GCAGCGATTC AATGTTTCTG GCTTGTCCTG TTTGCGCTCA ATAAAAGAGT CTTTGTTCGA
    AGAATATGGG TATTCTATGT GATCTATATG GCGATTCTGT ATCCACTTCA GTTCTTTTCA
    TATGTTGGAT TACCACCTGA CAGTTGTATT GAGTATCCAT GGTCGTATTG GATTCCATCG
    TATAGTGATG ATGCGAGATT CAATCTGTCG TATCTTCTGA ACTTGTCAAT ATACGGTGTA
    AACTGGCCAT CAGCATATCT CATTGGCGAT TTCTTCGTAC TCCTCTTGGC ATCATGCCAA
    TTAGCAGTTT TCCGGCGAGA AGGAGAAGAT AATGATTCAA TTTACAATGA TGGAAACTTT
    GTGATTAAGC CAGAAAATCC ACAATATGAC TTTATTGACA CCAAAAAGAG TTACGTTGAT
    TATTTCAAAT CATTCGTTTT CCATTATGGA CACTGGATTA CATTGATGTC TACACTAGCA
    GCAGGAATTG CTGGAACGTC ACTTTTTGCT TTGGGATACA TTATATTCAC TCTGACAATG
    CTGTGGTCTG GTAACAATTT GTATGTGATG AACTCAACTC TACGATCATT TGAACATACT
    TTGAAACGAT GGAATGCACT TTTGGGATAC ACATTGTTCA CGATTACAAT GAAAGTTTGC
    CTTCAAATAT TTGGATGTGT ATTCCTGTCG TGGTTCGATC AATCTGGTGG ATGGGGAAAG
    ACTCTGTGCA TCGTTCGTCA ACTCTTCAGT ATCACATGTG TTAATAACGA ATGTCATGTG
    CTCAAAGAGC TGGAGGATTT CTCGAAGGCG TGTGCTGTTG AGACGAAAGA AGGAAATATT
    GGATTTGATG TGATTGCGCT ATCCTTCCTC GTCTTCCAAA TCCGAATTTT CCATTCTTGG
    TATTTCCAAC ACTGCATGGT TGAATATCGA AGTGAGGTTA TTCTGGCCAA TAGAGGTGCC
    GTGCTGAAGA ATCAACTGAT TGAAAAGGAA ATGAAGGAAC AAAACGAGCA GCAAAAAGCC
    AAGTTCAATG ATATCAGAAG AAGAACTGAA GCTATTCGTG AAAGATATCA AAAACAAATC
    GAACGAGGAG CTGCTGAACG TGACTTCGAG CCGGTTACTT ATGGGCACGC CAAACGAGCG
    GGAGACTACT ACATGTTCAA ATACGATCCT GAAAATGATG ATCTTGTGGA ACCCGTCGAC
    AGTTTTGTAC CAGAAGTTGA TCCCAAGGCA ACTGCATATG ATCGACTTGA TCCTGGACAG
    ATAATGTACG CTGCCACCGC TCACGACCTC GACCTCGCCA AAACTGTACA GCAAGTCAAG
    AAAGGCGATA CTATCAAAGA TCCTGATTCA CGTGCTCTTA TCGCTGTTTC TGAACCAGAA
    GCTCGTAAGC CAGGAGGCAC TGAAGAAACG GATGGTGATG AAGATGAGGA TAATAAGGAT
    TCGAAAGTGG AAAGTACTGC TAAATTCATT CAAAAAATGA TCGCTTCTGC CTTGGACTTG
    TGTAGTGTAA CACTAAACAA ACTGTGCAGA GAACACCGAT ATGTTGGATT TGTTTTGAGC
    AAGGAAAAAC AAAAGTTGAA GTCAGGACAC TCGGAATCTC TTTCAAATAC ATCTAGAAAG
    CTGACGGATA TTCGATCTGC AGTTGATCTA CCAAGTCTTC AACTTGTGCA ATCGGCGAAT
    GATGTTGAAA AGATGGAGAC AGCCGTCAGC GTGGATTGGC AACAAAAATC ATCAGCAACG
    AGACTATTGA ATGCAGTTGT GAATTGTATT GGAGCACACA CTGATATTCT CTGCTACTTC
    TTCGCAATTA TGACACAAGT GATGACTGGT GGACTTATTA CTCTTCCACT TCCTCTGATG
    TCACTATTCT GGGGAAATCT ATCGAATCCT CGTCCATCCA AGTTCTTCTG GGTCACAATG
    ATCACCTACA CTGAGTGTGT CATTGTTATC AAGTTTGTTT GCCAATTTGC ATTCATGCCT
    TACAATTCTA TCACGTGGCG AACAGAGCAT CAAATGGACC CGATGTCATT GGATAAGTTA
    TTCGGAGTAT CACAGAGAGA TAGTTTTGCA CTTTGGGACA TTGTTTTGCT CTTCTCACTC
    TTCTTCCATC GGTATATGCT CAGAAAGTTA GGGTTATGGA AGGATGCCAA TTTGACGGAC
    ACGTTCACTT TGAAGGAAGA GCCTCGATCG GCGTCAGGAT CGGACACAGG ATCACCCAAG
    AAAATTGCTC AAGAGCCAAA GGTTGTTGTA ACTCAGTCAG ATACCCTTGA AGGTACAAGT
    GGAGGAGAGA TAGTCATACC ATCTGATCCA AATGCAGTTT CCAATATGGA AGAATTAGAC
    TGCGAACCTC CGATTCCAGA GAAGCAAAGT GGACCAATTG GAAGATTCAT TCATCAATTA
    TTCCATCCAA AATTCCGTTA CATTCGTGAT TTGTATCCAA TAATGTTTGG AATCGATGTG
    ATATGCTTCC TCATCATGAC ATTTGGATAC TCGGCATTTG GTGAAGGAGG TTCTGGAAAT
    GTTTTAGATG ATGTGAAGGC TTCTCGTATT CCGGTAACAC TTGTTGTTAT GCTCGTTGGA
    ATGACTCTGG CAATTATCAT TGATCGAGCA CTCTACCTCA GAAAATCAGT TGTCGGAAAG
    CTAATCTATC AAGTCTTAAT GATTGCTTTC CTTCACATTT GGGTATTCCT TGTTCTTCCA
    AATATGACTC GTCGTTCTGC GATATCGAAT CACGTTGCTC AAGCATTGTA CGTCATCAAA
    TCGTGCTATT TCCTTGTGTC TGCTTGGCAG ATCCGAAATG GTTATCCTGA ACTTTGTATT
    GGAAATCTAT TAACACATTC ATACGGAATG ACCAATATGA TTGCATTCAA AGTATTCATG
    AATATTCCAT TCCTCTTCGA GCTTCGTACA GCTATTGATT GGACATGGAC AGATACTTCA
    ATGCCTCTTT TCGATTTCTT CAATATGGAA AATTTCTATG CTCATATATT CAATATCAAG
    TGTGCCCGCC AGTTCGAAGC TGCCTATCCA GCTCCAAGAG GGATTCCCAA AGGAAAGTTG
    GTCAAGTATA TGATGGGATT CCCAATTATC ATTGGAGTCG TTATCTTCAT CTTTTCTCCT
    CTATTGCTCT GGTCGCTACT CAATCAAATC GGAACTATCA GTATGCCAGA AAAAGTTACG
    TTGAGGATTT CGATTGAAGG ATATCCACCA CTTTACGAGA TGGAGGCACA AGGAAGTAAT
    CACGATAACG CTGAACTTGG AATGATAAAA CCTGATCAAT TGGCATCTTT GAATCAAGCA
    TTGACGGATA GTTACACGAC ACGAGACACT AATTCTATTT TAAGATCAAG AATGTCTGTA
    TCATATCTGA AAGGATACAC ATATGAGGAT ATTCTGATTG TGCGATTCCG TCCAGAATCT
    GAAATTTATT GGCCGATTTC ACAGGATTCG AGAAATGCGA TGATCGATAA ATTGAGCCGA
    AACACGTCAG TCAATTTTGA AGTATCTCTG GAATTCACTC GGCCATATGA TCCAAATGAA
    AACGCTGCTT TAAAACATTC GAAATCGTGG CTTGTTCCAA TCTCCCTGGA TATGACGATT
    CGAGCAAAAA TTCAAAGTGC TCTCCGAGGA GATCCAGGTC ATCCGATTCT CATTCCACAG
    TCAATTCCTG CGTTTATTCA AGTTCCAAAT CAAGGAGAAT TGACCCTTCC AACATCAATC
    GGAAATACTA TTATCAATGA CGGAAATCCA CGGATTAATA CGACCGGGAT GGAAAAATCA
    GACGAAGCTC GGGCTTGGTT CGACTCGTTA ACCTTGAATC TCGAACAAGG AAAATCGCAA
    AACGAGAAAA TGTGGATCGC CACATCCGAA CATCCAGGAG ACCAAAATGC GAAACTCTGG
    ATAAAGACTG CAAATACGAC GTATTCGGGA AGGCCGTACC TTCAAGTTGT CGGATTTATT
    GATAGAGCAT TCCCATCATT TTTGGCGAAA GTTTTCAAAG GAGGAGTCAT TGCAGTGTAT
    CTTTCAGTTA TCCTCGTCGT TGGAAGAGGT CTGGTTCGTG GAATCTTCAC CACATCTCCA
    AGCACTGTCA TGTTCACAGA ATTACCGAAT GCGGATCATC TTCTCAAAAT TTGTCTCGAC
    ATCTATTTGG TTCGAGAAGC GAAGGACTTC ATGTTGGAGC AGGATCTCTT CGCCAAATTA
    ATATTCCTGT TCCGATCACC AGCGACACTC ATCGAATGGA CACGAATGAG CAAAAAGAAG
    CAAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343009
    Clone ID Related Accession (Same CDS sequence) NM_001307052.1 , NM_001307052.2
    Accession Version NM_001307052.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACTA CAGCACCCGG AGATAAGGAA TCTGCTGCTT CGAAAGGAAT GATTGCAGTC
    ATGACATTTG TCATTTTCCA TTCGTATTCA ATCGCGCTGA CTGCTATGAT GACATGGGCT
    CTCCTCTATC ATTCGATATT CGGTCTCATT CTCCTCATCC TCACATGTAT CTTGTGGATC
    TTCCGTGATA CGAGAAAGTC ATCGTTTGCA ATGGCCCCTA TTATTTTGAT GTATATCGAG
    TTTCTATTGA TTCTTCAATA TTTCCTCAGT ATGGATATTC ATGCAGAAAT TGGCGATCCA
    GCGTGGATGA ACTTTGTTGG AATCGAATGG ACTACTCTTC CAGTACATGC TGTCATCATT
    CTCTGTGTCC AGACTCTTCT CACTCTCCCA GTGTTCTTGC TTCTACGTTT GGCTCGCAGG
    GAGAAGTTTT ACGAATCGCT GAGTGATTAT GAACGACAAC GACGAATCAA CTCATATGGA
    ACATTTGGAG CATCAAAGAC TGGAGCTGGG GGTGTTGCAG TGGCTAAGTT TCAGGATCCA
    AAAAGTCGGA AGTTCGCGGC ATTTGTGGAA TATCTGTCGA ATAAAGTGTC TGTCTACTTC
    ATATTTGTAG TATCAGTGGT ACTTTTGGTG GTCTCTACGT GTTTTGCTCC GAATTTCTAC
    AATATTCTAT TCTTCGCACT ATGGGCATTG AACTTGATTT ATCTCAAGTT CTCATTCCGT
    CTGTATCGTG GATTGGCATA TGCATTCTGG CTAACTCTCA CGTTCTACAC TTCAATTGTT
    ATTATCGCCC TCTACATTTA TCAGTTCCCT GGAGTATCTC AATGGATTAT TCGAAATACT
    AGTTTGTCGC AGGAGTGGCT GAATGCAATT GGACTTGTCG ATTTTAGAGC TATCGGTGAA
    TCGGGAGCCC TATTTCTACA ATTACTCGCC CCAATTGCCT TGTTCGTCGT CACAATGCTC
    CAACTCAAGT TTTTCCATGG ACCATGGAGT CGAGCAACGT CACCAAGAAG AGCAGAAAAT
    GATCCACCTA CAAGTACGAC GGAGGCTGCG GCGGTAGCAA GCACATCTGG AACACAAGGA
    AGAGCACATG CTGCAGGCGA TACCCTTGTC AAGAAGCTGC ACAAATTGGC AAATCAGACA
    ATCGAACTGT TATGGAGGTT TTTCGAAGTT CACATTTCGA AAATTGTCTT TGTCATCATT
    GCGATTTTTA TTGCCAACAA TATCAATGCT CTATACATTC CACTTGTTAT TCTCCTCTCA
    TTGGCTATAT GTCTACCATC AGCTGCAGAT GGGATATTCT CTTTATTTAT GTGTGCCTAC
    TTATTCCTCG TGGCACTGAG CAAGATGATC TACCAACTGG ATATTGTTCC CGAGTTATCA
    CAAATCGACC GAGGAGTTGG AGCAGATAAC TGTTCACATG GAAATATTTC AATGCCTGAA
    TGGTTTGGAT TGAAGAAGGA AGTTGAAGGA ACTGAACCAA TTTATATGCT CTTTGGAGTC
    ATTGTTTCGA TTATTGCATT GGCTTTTCAA TCAATTGTTA TATATCGACA AAGACATTAT
    CGAGCTTCAC TGGGACTTCC TGAATCGATG CGAGCTAAAG TGTTCCCGGA CTTCCATCAT
    TCTCATTTTG ATAGATCTCT CAAAAATGCC ATCCAATTCT TGATAGACTA TGGTTTCTAT
    AAGTTTGGAC TTGAAATCAC GATGATCGCC ATTGGAATTG ACATTTTCAA TCGAATGGAT
    GCATTGGCAG CGATTCAATG TTTCTGGCTT GTCCTGTTTG CGCTCAATAA AAGAGTCTTT
    GTTCGAAGAA TATGGGTATT CTATGTGATC TATATGGCGA TTCTGTATCC ACTTCAGTTC
    TTTTCATATG TTGGATTACC ACCTGACAGT TGTATTGAGT ATCCATGGTC GTATTGGATT
    CCATCGTATA GTGATGATGC GAGATTCAAT CTGTCGTATC TTCTGAACTT GTCAATATAC
    GGTGTAAACT GGCCATCAGC ATATCTCATT GGCGATTTCT TCGTACTCCT CTTGGCATCA
    TGCCAATTAG CAGTTTTCCG GCGAGAAGGA GAAGATAATG ATTCAATTTA CAATGATGGA
    AACTTTGTGA TTAAGCCAGA AAATCCACAA TATGACTTTA TTGACACCAA AAAGAGTTAC
    GTTGATTATT TCAAATCATT CGTTTTCCAT TATGGACACT GGATTACATT GATGTCTACA
    CTAGCAGCAG GAATTGCTGG AACGTCACTT TTTGCTTTGG GATACATTAT ATTCACTCTG
    ACAATGCTGT GGTCTGGTAA CAATTTGTAT GTGATGAACT CAACTCTACG ATCATTTGAA
    CATACTTTGA AACGATGGAA TGCACTTTTG GGATACACAT TGTTCACGAT TACAATGAAA
    GTTTGCCTTC AAATATTTGG ATGTGTATTC CTGTCGTGGT TCGATCAATC TGGTGGATGG
    GGAAAGACTC TGTGCATCGT TCGTCAACTC TTCAGTATCA CATGTGTTAA TAACGAATGT
    CATGTGCTCA AAGAGCTGGA GGATTTCTCG AAGGCGTGTG CTGTTGAGAC GAAAGAAGGA
    AATATTGGAT TTGATGTGAT TGCGCTATCC TTCCTCGTCT TCCAAATCCG AATTTTCCAT
    TCTTGGTATT TCCAACACTG CATGGTTGAA TATCGAAGTG AGGTTATTCT GGCCAATAGA
    GGTGCCGTGC TGAAGAATCA ACTGATTGAA AAGGAAATGA AGGAACAAAA CGAGCAGCAA
    AAAGCCAAGT TCAATGATAT CAGAAGAAGA ACTGAAGCTA TTCGTGAAAG ATATCAAAAA
    CAAATCGAAC GAGGAGCTGC TGAACGTGAC TTCGAGCCGG TTACTTATGG GCACGCCAAA
    CGAGCGGGAG ACTACTACAT GTTCAAATAC GATCCTGAAA ATGATGATCT TGTGGAACCC
    GTCGACAGTT TTGTACCAGA AGTTGATCCC AAGGCAACTG CATATGATCG ACTTGATCCT
    GGACAGATAA TGTACGCTGC CACCGCTCAC GACCTCGACC TCGCCAAAAC TGTACAGCAA
    GTCAAGAAAG GCGATACTAT CAAAGATCCT GATTCACGTG CTCTTATCGC TGTTTCTGAA
    CCAGAAGCTC GTAAGCCAGG AGGCACTGAA GAAACGGATG GTGATGAAGA TGAGGATAAT
    AAGGATTCGA AAGTGGAAAG TACTGCTAAA TTCATTCAAA AAATGATCGC TTCTGCCTTG
    GACTTGTGTA GTGTAACACT AAACAAACTG TGCAGAGAAC ACCGATATGT TGGATTTGTT
    TTGAGCAAGG AAAAACAAAA GTTGAAGTCA GGACACTCGG AATCTCTTTC AAATACATCT
    AGAAAGCTGA CGGATATTCG ATCTGCAGTT GATCTACCAA GTCTTCAACT TGTGCAATCG
    GCGAATGATG TTGAAAAGAT GGAGACAGCC GTCAGCGTGG ATTGGCAACA AAAATCATCA
    GCAACGAGAC TATTGAATGC AGTTGTGAAT TGTATTGGAG CACACACTGA TATTCTCTGC
    TACTTCTTCG CAATTATGAC ACAAGTGATG ACTGGTGGAC TTATTACTCT TCCACTTCCT
    CTGATGTCAC TATTCTGGGG AAATCTATCG AATCCTCGTC CATCCAAGTT CTTCTGGGTC
    ACAATGATCA CCTACACTGA GTGTGTCATT GTTATCAAGT TTGTTTGCCA ATTTGCATTC
    ATGCCTTACA ATTCTATCAC GTGGCGAACA GAGCATCAAA TGGACCCGAT GTCATTGGAT
    AAGTTATTCG GAGTATCACA GAGAGATAGT TTTGCACTTT GGGACATTGT TTTGCTCTTC
    TCACTCTTCT TCCATCGGTA TATGCTCAGA AAGTTAGGGT TATGGAAGGA TGCCAATTTG
    ACGGACACGT TCACTTTGAA GGAAGAGCCT CGATCGGCGT CAGGATCGGA CACAGGATCA
    CCCAAGAAAA TTGCTCAAGA GCCAAAGGTT GTTGTAACTC AGTCAGATAC CCTTGAAGGT
    ACAAGTGGAG GAGAGATAGT CATACCATCT GATCCAAATG CAGTTTCCAA TATGGAAGAA
    TTAGACTGCG AACCTCCGAT TCCAGAGAAG CAAAGTGGAC CAATTGGAAG ATTCATTCAT
    CAATTATTCC ATCCAAAATT CCGTTACATT CGTGATTTGT ATCCAATAAT GTTTGGAATC
    GATGTGATAT GCTTCCTCAT CATGACATTT GGATACTCGG CATTTGGTGA AGGAGGTTCT
    GGAAATGTTT TAGATGATGT GAAGGCTTCT CGTATTCCGG TAACACTTGT TGTTATGCTC
    GTTGGAATGA CTCTGGCAAT TATCATTGAT CGAGCACTCT ACCTCAGAAA ATCAGTTGTC
    GGAAAGCTAA TCTATCAAGT CTTAATGATT GCTTTCCTTC ACATTTGGGT ATTCCTTGTT
    CTTCCAAATA TGACTCGTCG TTCTGCGATA TCGAATCACG TTGCTCAAGC ATTGTACGTC
    ATCAAATCGT GCTATTTCCT TGTGTCTGCT TGGCAGATCC GAAATGGTTA TCCTGAACTT
    TGTATTGGAA ATCTATTAAC ACATTCATAC GGAATGACCA ATATGATTGC ATTCAAAGTA
    TTCATGAATA TTCCATTCCT CTTCGAGCTT CGTACAGCTA TTGATTGGAC ATGGACAGAT
    ACTTCAATGC CTCTTTTCGA TTTCTTCAAT ATGGAAAATT TCTATGCTCA TATATTCAAT
    ATCAAGTGTG CCCGCCAGTT CGAAGCTGCC TATCCAGCTC CAAGAGGGAT TCCCAAAGGA
    AAGTTGGTCA AGTATATGAT GGGATTCCCA ATTATCATTG GAGTCGTTAT CTTCATCTTT
    TCTCCTCTAT TGCTCTGGTC GCTACTCAAT CAAATCGGAA CTATCAGTAT GCCAGAAAAA
    GTTACGTTGA GGATTTCGAT TGAAGGATAT CCACCACTTT ACGAGATGGA GGCACAAGGA
    AGTAATCACG ATAACGCTGA ACTTGGAATG ATAAAACCTG ATCAATTGGC ATCTTTGAAT
    CAAGCATTGA CGGATAGTTA CACGACACGA GACACTAATT CTATTTTAAG ATCAAGAATG
    TCTGTATCAT ATCTGAAAGG ATACACATAT GAGGATATTC TGATTGTGCG ATTCCGTCCA
    GAATCTGAAA TTTATTGGCC GATTTCACAG GATTCGAGAA ATGCGATGAT CGATAAATTG
    AGCCGAAACA CGTCAGTCAA TTTTGAAGTA TCTCTGGAAT TCACTCGGCC ATATGATCCA
    AATGAAAACG CTGCTTTAAA ACATTCGAAA TCGTGGCTTG TTCCAATCTC CCTGGATATG
    ACGATTCGAG CAAAAATTCA AAGTGCTCTC CGAGGAGATC CAGGTCATCC GATTCTCATT
    CCACAGTCAA TTCCTGCGTT TATTCAAGTT CCAAATCAAG GAGAATTGAC CCTTCCAACA
    TCAATCGGAA ATACTATTAT CAATGACGGA AATCCACGGA TTAATACGAC CGGGATGGAA
    AAATCAGACG AAGCTCGGGC TTGGTTCGAC TCGTTAACCT TGAATCTCGA ACAAGGAAAA
    TCGCAAAACG AGAAAATGTG GATCGCCACA TCCGAACATC CAGGAGACCA AAATGCGAAA
    CTCTGGATAA AGACTGCAAA TACGACGTAT TCGGGAAGGC CGTACCTTCA AGTTGTCGGA
    TTTATTGATA GAGCATTCCC ATCATTTTTG GCGAAAGTTT TCAAAGGAGG AGTCATTGCA
    GTGTATCTTT CAGTTATCCT CGTCGTTGGA AGAGGTCTGG TTCGTGGAAT CTTCACCACA
    TCTCCAAGCA CTGTCATGTT CACAGAATTA CCGAATGCGG ATCATCTTCT CAAAATTTGT
    CTCGACATCT ATTTGGTTCG AGAAGCGAAG GACTTCATGT TGGAGCAGGA TCTCTTCGCC
    AAATTAATAT TCCTGTTCCG ATCACCAGCG ACACTCATCG AATGGACACG AATGAGCAAA
    AAGAAGCAAG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293981.1
    CDS1..7215
    Translation

    Target ORF information:

    RefSeq Version NM_001307052.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307052.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACTA CAGCACCCGG AGATAAGGAA TCTGCTGCTT CGAAAGGAAT GATTGCAGTC
    ATGACATTTG TCATTTTCCA TTCGTATTCA ATCGCGCTGA CTGCTATGAT GACATGGGCT
    CTCCTCTATC ATTCGATATT CGGTCTCATT CTCCTCATCC TCACATGTAT CTTGTGGATC
    TTCCGTGATA CGAGAAAGTC ATCGTTTGCA ATGGCCCCTA TTATTTTGAT GTATATCGAG
    TTTCTATTGA TTCTTCAATA TTTCCTCAGT ATGGATATTC ATGCAGAAAT TGGCGATCCA
    GCGTGGATGA ACTTTGTTGG AATCGAATGG ACTACTCTTC CAGTACATGC TGTCATCATT
    CTCTGTGTCC AGACTCTTCT CACTCTCCCA GTGTTCTTGC TTCTACGTTT GGCTCGCAGG
    GAGAAGTTTT ACGAATCGCT GAGTGATTAT GAACGACAAC GACGAATCAA CTCATATGGA
    ACATTTGGAG CATCAAAGAC TGGAGCTGGG GGTGTTGCAG TGGCTAAGTT TCAGGATCCA
    AAAAGTCGGA AGTTCGCGGC ATTTGTGGAA TATCTGTCGA ATAAAGTGTC TGTCTACTTC
    ATATTTGTAG TATCAGTGGT ACTTTTGGTG GTCTCTACGT GTTTTGCTCC GAATTTCTAC
    AATATTCTAT TCTTCGCACT ATGGGCATTG AACTTGATTT ATCTCAAGTT CTCATTCCGT
    CTGTATCGTG GATTGGCATA TGCATTCTGG CTAACTCTCA CGTTCTACAC TTCAATTGTT
    ATTATCGCCC TCTACATTTA TCAGTTCCCT GGAGTATCTC AATGGATTAT TCGAAATACT
    AGTTTGTCGC AGGAGTGGCT GAATGCAATT GGACTTGTCG ATTTTAGAGC TATCGGTGAA
    TCGGGAGCCC TATTTCTACA ATTACTCGCC CCAATTGCCT TGTTCGTCGT CACAATGCTC
    CAACTCAAGT TTTTCCATGG ACCATGGAGT CGAGCAACGT CACCAAGAAG AGCAGAAAAT
    GATCCACCTA CAAGTACGAC GGAGGCTGCG GCGGTAGCAA GCACATCTGG AACACAAGGA
    AGAGCACATG CTGCAGGCGA TACCCTTGTC AAGAAGCTGC ACAAATTGGC AAATCAGACA
    ATCGAACTGT TATGGAGGTT TTTCGAAGTT CACATTTCGA AAATTGTCTT TGTCATCATT
    GCGATTTTTA TTGCCAACAA TATCAATGCT CTATACATTC CACTTGTTAT TCTCCTCTCA
    TTGGCTATAT GTCTACCATC AGCTGCAGAT GGGATATTCT CTTTATTTAT GTGTGCCTAC
    TTATTCCTCG TGGCACTGAG CAAGATGATC TACCAACTGG ATATTGTTCC CGAGTTATCA
    CAAATCGACC GAGGAGTTGG AGCAGATAAC TGTTCACATG GAAATATTTC AATGCCTGAA
    TGGTTTGGAT TGAAGAAGGA AGTTGAAGGA ACTGAACCAA TTTATATGCT CTTTGGAGTC
    ATTGTTTCGA TTATTGCATT GGCTTTTCAA TCAATTGTTA TATATCGACA AAGACATTAT
    CGAGCTTCAC TGGGACTTCC TGAATCGATG CGAGCTAAAG TGTTCCCGGA CTTCCATCAT
    TCTCATTTTG ATAGATCTCT CAAAAATGCC ATCCAATTCT TGATAGACTA TGGTTTCTAT
    AAGTTTGGAC TTGAAATCAC GATGATCGCC ATTGGAATTG ACATTTTCAA TCGAATGGAT
    GCATTGGCAG CGATTCAATG TTTCTGGCTT GTCCTGTTTG CGCTCAATAA AAGAGTCTTT
    GTTCGAAGAA TATGGGTATT CTATGTGATC TATATGGCGA TTCTGTATCC ACTTCAGTTC
    TTTTCATATG TTGGATTACC ACCTGACAGT TGTATTGAGT ATCCATGGTC GTATTGGATT
    CCATCGTATA GTGATGATGC GAGATTCAAT CTGTCGTATC TTCTGAACTT GTCAATATAC
    GGTGTAAACT GGCCATCAGC ATATCTCATT GGCGATTTCT TCGTACTCCT CTTGGCATCA
    TGCCAATTAG CAGTTTTCCG GCGAGAAGGA GAAGATAATG ATTCAATTTA CAATGATGGA
    AACTTTGTGA TTAAGCCAGA AAATCCACAA TATGACTTTA TTGACACCAA AAAGAGTTAC
    GTTGATTATT TCAAATCATT CGTTTTCCAT TATGGACACT GGATTACATT GATGTCTACA
    CTAGCAGCAG GAATTGCTGG AACGTCACTT TTTGCTTTGG GATACATTAT ATTCACTCTG
    ACAATGCTGT GGTCTGGTAA CAATTTGTAT GTGATGAACT CAACTCTACG ATCATTTGAA
    CATACTTTGA AACGATGGAA TGCACTTTTG GGATACACAT TGTTCACGAT TACAATGAAA
    GTTTGCCTTC AAATATTTGG ATGTGTATTC CTGTCGTGGT TCGATCAATC TGGTGGATGG
    GGAAAGACTC TGTGCATCGT TCGTCAACTC TTCAGTATCA CATGTGTTAA TAACGAATGT
    CATGTGCTCA AAGAGCTGGA GGATTTCTCG AAGGCGTGTG CTGTTGAGAC GAAAGAAGGA
    AATATTGGAT TTGATGTGAT TGCGCTATCC TTCCTCGTCT TCCAAATCCG AATTTTCCAT
    TCTTGGTATT TCCAACACTG CATGGTTGAA TATCGAAGTG AGGTTATTCT GGCCAATAGA
    GGTGCCGTGC TGAAGAATCA ACTGATTGAA AAGGAAATGA AGGAACAAAA CGAGCAGCAA
    AAAGCCAAGT TCAATGATAT CAGAAGAAGA ACTGAAGCTA TTCGTGAAAG ATATCAAAAA
    CAAATCGAAC GAGGAGCTGC TGAACGTGAC TTCGAGCCGG TTACTTATGG GCACGCCAAA
    CGAGCGGGAG ACTACTACAT GTTCAAATAC GATCCTGAAA ATGATGATCT TGTGGAACCC
    GTCGACAGTT TTGTACCAGA AGTTGATCCC AAGGCAACTG CATATGATCG ACTTGATCCT
    GGACAGATAA TGTACGCTGC CACCGCTCAC GACCTCGACC TCGCCAAAAC TGTACAGCAA
    GTCAAGAAAG GCGATACTAT CAAAGATCCT GATTCACGTG CTCTTATCGC TGTTTCTGAA
    CCAGAAGCTC GTAAGCCAGG AGGCACTGAA GAAACGGATG GTGATGAAGA TGAGGATAAT
    AAGGATTCGA AAGTGGAAAG TACTGCTAAA TTCATTCAAA AAATGATCGC TTCTGCCTTG
    GACTTGTGTA GTGTAACACT AAACAAACTG TGCAGAGAAC ACCGATATGT TGGATTTGTT
    TTGAGCAAGG AAAAACAAAA GTTGAAGTCA GGACACTCGG AATCTCTTTC AAATACATCT
    AGAAAGCTGA CGGATATTCG ATCTGCAGTT GATCTACCAA GTCTTCAACT TGTGCAATCG
    GCGAATGATG TTGAAAAGAT GGAGACAGCC GTCAGCGTGG ATTGGCAACA AAAATCATCA
    GCAACGAGAC TATTGAATGC AGTTGTGAAT TGTATTGGAG CACACACTGA TATTCTCTGC
    TACTTCTTCG CAATTATGAC ACAAGTGATG ACTGGTGGAC TTATTACTCT TCCACTTCCT
    CTGATGTCAC TATTCTGGGG AAATCTATCG AATCCTCGTC CATCCAAGTT CTTCTGGGTC
    ACAATGATCA CCTACACTGA GTGTGTCATT GTTATCAAGT TTGTTTGCCA ATTTGCATTC
    ATGCCTTACA ATTCTATCAC GTGGCGAACA GAGCATCAAA TGGACCCGAT GTCATTGGAT
    AAGTTATTCG GAGTATCACA GAGAGATAGT TTTGCACTTT GGGACATTGT TTTGCTCTTC
    TCACTCTTCT TCCATCGGTA TATGCTCAGA AAGTTAGGGT TATGGAAGGA TGCCAATTTG
    ACGGACACGT TCACTTTGAA GGAAGAGCCT CGATCGGCGT CAGGATCGGA CACAGGATCA
    CCCAAGAAAA TTGCTCAAGA GCCAAAGGTT GTTGTAACTC AGTCAGATAC CCTTGAAGGT
    ACAAGTGGAG GAGAGATAGT CATACCATCT GATCCAAATG CAGTTTCCAA TATGGAAGAA
    TTAGACTGCG AACCTCCGAT TCCAGAGAAG CAAAGTGGAC CAATTGGAAG ATTCATTCAT
    CAATTATTCC ATCCAAAATT CCGTTACATT CGTGATTTGT ATCCAATAAT GTTTGGAATC
    GATGTGATAT GCTTCCTCAT CATGACATTT GGATACTCGG CATTTGGTGA AGGAGGTTCT
    GGAAATGTTT TAGATGATGT GAAGGCTTCT CGTATTCCGG TAACACTTGT TGTTATGCTC
    GTTGGAATGA CTCTGGCAAT TATCATTGAT CGAGCACTCT ACCTCAGAAA ATCAGTTGTC
    GGAAAGCTAA TCTATCAAGT CTTAATGATT GCTTTCCTTC ACATTTGGGT ATTCCTTGTT
    CTTCCAAATA TGACTCGTCG TTCTGCGATA TCGAATCACG TTGCTCAAGC ATTGTACGTC
    ATCAAATCGT GCTATTTCCT TGTGTCTGCT TGGCAGATCC GAAATGGTTA TCCTGAACTT
    TGTATTGGAA ATCTATTAAC ACATTCATAC GGAATGACCA ATATGATTGC ATTCAAAGTA
    TTCATGAATA TTCCATTCCT CTTCGAGCTT CGTACAGCTA TTGATTGGAC ATGGACAGAT
    ACTTCAATGC CTCTTTTCGA TTTCTTCAAT ATGGAAAATT TCTATGCTCA TATATTCAAT
    ATCAAGTGTG CCCGCCAGTT CGAAGCTGCC TATCCAGCTC CAAGAGGGAT TCCCAAAGGA
    AAGTTGGTCA AGTATATGAT GGGATTCCCA ATTATCATTG GAGTCGTTAT CTTCATCTTT
    TCTCCTCTAT TGCTCTGGTC GCTACTCAAT CAAATCGGAA CTATCAGTAT GCCAGAAAAA
    GTTACGTTGA GGATTTCGAT TGAAGGATAT CCACCACTTT ACGAGATGGA GGCACAAGGA
    AGTAATCACG ATAACGCTGA ACTTGGAATG ATAAAACCTG ATCAATTGGC ATCTTTGAAT
    CAAGCATTGA CGGATAGTTA CACGACACGA GACACTAATT CTATTTTAAG ATCAAGAATG
    TCTGTATCAT ATCTGAAAGG ATACACATAT GAGGATATTC TGATTGTGCG ATTCCGTCCA
    GAATCTGAAA TTTATTGGCC GATTTCACAG GATTCGAGAA ATGCGATGAT CGATAAATTG
    AGCCGAAACA CGTCAGTCAA TTTTGAAGTA TCTCTGGAAT TCACTCGGCC ATATGATCCA
    AATGAAAACG CTGCTTTAAA ACATTCGAAA TCGTGGCTTG TTCCAATCTC CCTGGATATG
    ACGATTCGAG CAAAAATTCA AAGTGCTCTC CGAGGAGATC CAGGTCATCC GATTCTCATT
    CCACAGTCAA TTCCTGCGTT TATTCAAGTT CCAAATCAAG GAGAATTGAC CCTTCCAACA
    TCAATCGGAA ATACTATTAT CAATGACGGA AATCCACGGA TTAATACGAC CGGGATGGAA
    AAATCAGACG AAGCTCGGGC TTGGTTCGAC TCGTTAACCT TGAATCTCGA ACAAGGAAAA
    TCGCAAAACG AGAAAATGTG GATCGCCACA TCCGAACATC CAGGAGACCA AAATGCGAAA
    CTCTGGATAA AGACTGCAAA TACGACGTAT TCGGGAAGGC CGTACCTTCA AGTTGTCGGA
    TTTATTGATA GAGCATTCCC ATCATTTTTG GCGAAAGTTT TCAAAGGAGG AGTCATTGCA
    GTGTATCTTT CAGTTATCCT CGTCGTTGGA AGAGGTCTGG TTCGTGGAAT CTTCACCACA
    TCTCCAAGCA CTGTCATGTT CACAGAATTA CCGAATGCGG ATCATCTTCT CAAAATTTGT
    CTCGACATCT ATTTGGTTCG AGAAGCGAAG GACTTCATGT TGGAGCAGGA TCTCTTCGCC
    AAATTAATAT TCCTGTTCCG ATCACCAGCG ACACTCATCG AATGGACACG AATGAGCAAA
    AAGAAGCAAG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343009
    Clone ID Related Accession (Same CDS sequence) NM_001307052.1 , NM_001307052.2
    Accession Version NM_001307052.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Piezo-type mechanosensitive ion channel component
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307052.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACTA CAGCACCCGG AGATAAGGAA TCTGCTGCTT CGAAAGGAAT GATTGCAGTC
    ATGACATTTG TCATTTTCCA TTCGTATTCA ATCGCGCTGA CTGCTATGAT GACATGGGCT
    CTCCTCTATC ATTCGATATT CGGTCTCATT CTCCTCATCC TCACATGTAT CTTGTGGATC
    TTCCGTGATA CGAGAAAGTC ATCGTTTGCA ATGGCCCCTA TTATTTTGAT GTATATCGAG
    TTTCTATTGA TTCTTCAATA TTTCCTCAGT ATGGATATTC ATGCAGAAAT TGGCGATCCA
    GCGTGGATGA ACTTTGTTGG AATCGAATGG ACTACTCTTC CAGTACATGC TGTCATCATT
    CTCTGTGTCC AGACTCTTCT CACTCTCCCA GTGTTCTTGC TTCTACGTTT GGCTCGCAGG
    GAGAAGTTTT ACGAATCGCT GAGTGATTAT GAACGACAAC GACGAATCAA CTCATATGGA
    ACATTTGGAG CATCAAAGAC TGGAGCTGGG GGTGTTGCAG TGGCTAAGTT TCAGGATCCA
    AAAAGTCGGA AGTTCGCGGC ATTTGTGGAA TATCTGTCGA ATAAAGTGTC TGTCTACTTC
    ATATTTGTAG TATCAGTGGT ACTTTTGGTG GTCTCTACGT GTTTTGCTCC GAATTTCTAC
    AATATTCTAT TCTTCGCACT ATGGGCATTG AACTTGATTT ATCTCAAGTT CTCATTCCGT
    CTGTATCGTG GATTGGCATA TGCATTCTGG CTAACTCTCA CGTTCTACAC TTCAATTGTT
    ATTATCGCCC TCTACATTTA TCAGTTCCCT GGAGTATCTC AATGGATTAT TCGAAATACT
    AGTTTGTCGC AGGAGTGGCT GAATGCAATT GGACTTGTCG ATTTTAGAGC TATCGGTGAA
    TCGGGAGCCC TATTTCTACA ATTACTCGCC CCAATTGCCT TGTTCGTCGT CACAATGCTC
    CAACTCAAGT TTTTCCATGG ACCATGGAGT CGAGCAACGT CACCAAGAAG AGCAGAAAAT
    GATCCACCTA CAAGTACGAC GGAGGCTGCG GCGGTAGCAA GCACATCTGG AACACAAGGA
    AGAGCACATG CTGCAGGCGA TACCCTTGTC AAGAAGCTGC ACAAATTGGC AAATCAGACA
    ATCGAACTGT TATGGAGGTT TTTCGAAGTT CACATTTCGA AAATTGTCTT TGTCATCATT
    GCGATTTTTA TTGCCAACAA TATCAATGCT CTATACATTC CACTTGTTAT TCTCCTCTCA
    TTGGCTATAT GTCTACCATC AGCTGCAGAT GGGATATTCT CTTTATTTAT GTGTGCCTAC
    TTATTCCTCG TGGCACTGAG CAAGATGATC TACCAACTGG ATATTGTTCC CGAGTTATCA
    CAAATCGACC GAGGAGTTGG AGCAGATAAC TGTTCACATG GAAATATTTC AATGCCTGAA
    TGGTTTGGAT TGAAGAAGGA AGTTGAAGGA ACTGAACCAA TTTATATGCT CTTTGGAGTC
    ATTGTTTCGA TTATTGCATT GGCTTTTCAA TCAATTGTTA TATATCGACA AAGACATTAT
    CGAGCTTCAC TGGGACTTCC TGAATCGATG CGAGCTAAAG TGTTCCCGGA CTTCCATCAT
    TCTCATTTTG ATAGATCTCT CAAAAATGCC ATCCAATTCT TGATAGACTA TGGTTTCTAT
    AAGTTTGGAC TTGAAATCAC GATGATCGCC ATTGGAATTG ACATTTTCAA TCGAATGGAT
    GCATTGGCAG CGATTCAATG TTTCTGGCTT GTCCTGTTTG CGCTCAATAA AAGAGTCTTT
    GTTCGAAGAA TATGGGTATT CTATGTGATC TATATGGCGA TTCTGTATCC ACTTCAGTTC
    TTTTCATATG TTGGATTACC ACCTGACAGT TGTATTGAGT ATCCATGGTC GTATTGGATT
    CCATCGTATA GTGATGATGC GAGATTCAAT CTGTCGTATC TTCTGAACTT GTCAATATAC
    GGTGTAAACT GGCCATCAGC ATATCTCATT GGCGATTTCT TCGTACTCCT CTTGGCATCA
    TGCCAATTAG CAGTTTTCCG GCGAGAAGGA GAAGATAATG ATTCAATTTA CAATGATGGA
    AACTTTGTGA TTAAGCCAGA AAATCCACAA TATGACTTTA TTGACACCAA AAAGAGTTAC
    GTTGATTATT TCAAATCATT CGTTTTCCAT TATGGACACT GGATTACATT GATGTCTACA
    CTAGCAGCAG GAATTGCTGG AACGTCACTT TTTGCTTTGG GATACATTAT ATTCACTCTG
    ACAATGCTGT GGTCTGGTAA CAATTTGTAT GTGATGAACT CAACTCTACG ATCATTTGAA
    CATACTTTGA AACGATGGAA TGCACTTTTG GGATACACAT TGTTCACGAT TACAATGAAA
    GTTTGCCTTC AAATATTTGG ATGTGTATTC CTGTCGTGGT TCGATCAATC TGGTGGATGG
    GGAAAGACTC TGTGCATCGT TCGTCAACTC TTCAGTATCA CATGTGTTAA TAACGAATGT
    CATGTGCTCA AAGAGCTGGA GGATTTCTCG AAGGCGTGTG CTGTTGAGAC GAAAGAAGGA
    AATATTGGAT TTGATGTGAT TGCGCTATCC TTCCTCGTCT TCCAAATCCG AATTTTCCAT
    TCTTGGTATT TCCAACACTG CATGGTTGAA TATCGAAGTG AGGTTATTCT GGCCAATAGA
    GGTGCCGTGC TGAAGAATCA ACTGATTGAA AAGGAAATGA AGGAACAAAA CGAGCAGCAA
    AAAGCCAAGT TCAATGATAT CAGAAGAAGA ACTGAAGCTA TTCGTGAAAG ATATCAAAAA
    CAAATCGAAC GAGGAGCTGC TGAACGTGAC TTCGAGCCGG TTACTTATGG GCACGCCAAA
    CGAGCGGGAG ACTACTACAT GTTCAAATAC GATCCTGAAA ATGATGATCT TGTGGAACCC
    GTCGACAGTT TTGTACCAGA AGTTGATCCC AAGGCAACTG CATATGATCG ACTTGATCCT
    GGACAGATAA TGTACGCTGC CACCGCTCAC GACCTCGACC TCGCCAAAAC TGTACAGCAA
    GTCAAGAAAG GCGATACTAT CAAAGATCCT GATTCACGTG CTCTTATCGC TGTTTCTGAA
    CCAGAAGCTC GTAAGCCAGG AGGCACTGAA GAAACGGATG GTGATGAAGA TGAGGATAAT
    AAGGATTCGA AAGTGGAAAG TACTGCTAAA TTCATTCAAA AAATGATCGC TTCTGCCTTG
    GACTTGTGTA GTGTAACACT AAACAAACTG TGCAGAGAAC ACCGATATGT TGGATTTGTT
    TTGAGCAAGG AAAAACAAAA GTTGAAGTCA GGACACTCGG AATCTCTTTC AAATACATCT
    AGAAAGCTGA CGGATATTCG ATCTGCAGTT GATCTACCAA GTCTTCAACT TGTGCAATCG
    GCGAATGATG TTGAAAAGAT GGAGACAGCC GTCAGCGTGG ATTGGCAACA AAAATCATCA
    GCAACGAGAC TATTGAATGC AGTTGTGAAT TGTATTGGAG CACACACTGA TATTCTCTGC
    TACTTCTTCG CAATTATGAC ACAAGTGATG ACTGGTGGAC TTATTACTCT TCCACTTCCT
    CTGATGTCAC TATTCTGGGG AAATCTATCG AATCCTCGTC CATCCAAGTT CTTCTGGGTC
    ACAATGATCA CCTACACTGA GTGTGTCATT GTTATCAAGT TTGTTTGCCA ATTTGCATTC
    ATGCCTTACA ATTCTATCAC GTGGCGAACA GAGCATCAAA TGGACCCGAT GTCATTGGAT
    AAGTTATTCG GAGTATCACA GAGAGATAGT TTTGCACTTT GGGACATTGT TTTGCTCTTC
    TCACTCTTCT TCCATCGGTA TATGCTCAGA AAGTTAGGGT TATGGAAGGA TGCCAATTTG
    ACGGACACGT TCACTTTGAA GGAAGAGCCT CGATCGGCGT CAGGATCGGA CACAGGATCA
    CCCAAGAAAA TTGCTCAAGA GCCAAAGGTT GTTGTAACTC AGTCAGATAC CCTTGAAGGT
    ACAAGTGGAG GAGAGATAGT CATACCATCT GATCCAAATG CAGTTTCCAA TATGGAAGAA
    TTAGACTGCG AACCTCCGAT TCCAGAGAAG CAAAGTGGAC CAATTGGAAG ATTCATTCAT
    CAATTATTCC ATCCAAAATT CCGTTACATT CGTGATTTGT ATCCAATAAT GTTTGGAATC
    GATGTGATAT GCTTCCTCAT CATGACATTT GGATACTCGG CATTTGGTGA AGGAGGTTCT
    GGAAATGTTT TAGATGATGT GAAGGCTTCT CGTATTCCGG TAACACTTGT TGTTATGCTC
    GTTGGAATGA CTCTGGCAAT TATCATTGAT CGAGCACTCT ACCTCAGAAA ATCAGTTGTC
    GGAAAGCTAA TCTATCAAGT CTTAATGATT GCTTTCCTTC ACATTTGGGT ATTCCTTGTT
    CTTCCAAATA TGACTCGTCG TTCTGCGATA TCGAATCACG TTGCTCAAGC ATTGTACGTC
    ATCAAATCGT GCTATTTCCT TGTGTCTGCT TGGCAGATCC GAAATGGTTA TCCTGAACTT
    TGTATTGGAA ATCTATTAAC ACATTCATAC GGAATGACCA ATATGATTGC ATTCAAAGTA
    TTCATGAATA TTCCATTCCT CTTCGAGCTT CGTACAGCTA TTGATTGGAC ATGGACAGAT
    ACTTCAATGC CTCTTTTCGA TTTCTTCAAT ATGGAAAATT TCTATGCTCA TATATTCAAT
    ATCAAGTGTG CCCGCCAGTT CGAAGCTGCC TATCCAGCTC CAAGAGGGAT TCCCAAAGGA
    AAGTTGGTCA AGTATATGAT GGGATTCCCA ATTATCATTG GAGTCGTTAT CTTCATCTTT
    TCTCCTCTAT TGCTCTGGTC GCTACTCAAT CAAATCGGAA CTATCAGTAT GCCAGAAAAA
    GTTACGTTGA GGATTTCGAT TGAAGGATAT CCACCACTTT ACGAGATGGA GGCACAAGGA
    AGTAATCACG ATAACGCTGA ACTTGGAATG ATAAAACCTG ATCAATTGGC ATCTTTGAAT
    CAAGCATTGA CGGATAGTTA CACGACACGA GACACTAATT CTATTTTAAG ATCAAGAATG
    TCTGTATCAT ATCTGAAAGG ATACACATAT GAGGATATTC TGATTGTGCG ATTCCGTCCA
    GAATCTGAAA TTTATTGGCC GATTTCACAG GATTCGAGAA ATGCGATGAT CGATAAATTG
    AGCCGAAACA CGTCAGTCAA TTTTGAAGTA TCTCTGGAAT TCACTCGGCC ATATGATCCA
    AATGAAAACG CTGCTTTAAA ACATTCGAAA TCGTGGCTTG TTCCAATCTC CCTGGATATG
    ACGATTCGAG CAAAAATTCA AAGTGCTCTC CGAGGAGATC CAGGTCATCC GATTCTCATT
    CCACAGTCAA TTCCTGCGTT TATTCAAGTT CCAAATCAAG GAGAATTGAC CCTTCCAACA
    TCAATCGGAA ATACTATTAT CAATGACGGA AATCCACGGA TTAATACGAC CGGGATGGAA
    AAATCAGACG AAGCTCGGGC TTGGTTCGAC TCGTTAACCT TGAATCTCGA ACAAGGAAAA
    TCGCAAAACG AGAAAATGTG GATCGCCACA TCCGAACATC CAGGAGACCA AAATGCGAAA
    CTCTGGATAA AGACTGCAAA TACGACGTAT TCGGGAAGGC CGTACCTTCA AGTTGTCGGA
    TTTATTGATA GAGCATTCCC ATCATTTTTG GCGAAAGTTT TCAAAGGAGG AGTCATTGCA
    GTGTATCTTT CAGTTATCCT CGTCGTTGGA AGAGGTCTGG TTCGTGGAAT CTTCACCACA
    TCTCCAAGCA CTGTCATGTT CACAGAATTA CCGAATGCGG ATCATCTTCT CAAAATTTGT
    CTCGACATCT ATTTGGTTCG AGAAGCGAAG GACTTCATGT TGGAGCAGGA TCTCTTCGCC
    AAATTAATAT TCCTGTTCCG ATCACCAGCG ACACTCATCG AATGGACACG AATGAGCAAA
    AAGAAGCAAG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293981.1
    CDS57..7271
    Translation

    Target ORF information:

    RefSeq Version NM_001307052.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Piezo-type mechanosensitive ion channel component (pezo-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307052.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGACGGTCC CGCCGCTTTT AAAATCGTGC GTCGTCAAAT TGCTGCTTCC GGCAGCACTG 
    CTTGCAGCTG CCATAATCCG GCCGAGCTTT TTATCAATTG GCTACGTCCT ACTTGCACTT
    GTCAGCGCCG TTCTGCCACC AATTCGAAAG TCTTTAGCAC TTCCCAAACT AGTTGGAACA
    TTCGTTATTA TCACTTTTCT CTTCTGCCTA GCAGTCGCAC TAGGAGTTGG TTCCTACCAG
    ATCTCAGAAC AAGTTGTTCA TAAAAATGAC CGGACATACA TTTGCAATCG ATCCGACACT
    ACTCTGTTCC GGAGCATTGG CCTTGTCCGA TTCCATCCAA CAGGAACATT TGAATCAACT
    CGAGCATTTC TTCCGGAGAT TATTGCCACG TCTGCAGCTC TGCTCACTAT CATAATCGTC
    ATGTTTTTGT CGCATAGAGA CGAGCAATTG GACGTCGTTG GTGATGTTGT AACAGTCAGG
    AGTGAATCTG GCCGCGAGCA ACGACGACAA AGAAAGTTAG CCGCTATAAT GTGGAGTGCA
    ATCGGAAATT CTCTACGAAG ATTGACTAAT TTCGTGCTTT TCCTGTTCAC GGCATATGTT
    GGAATCGTGA AACCATCGCT GAGTAACTCA ATTTACTTTC TCGCTTTTCT GTTCATATCC
    ACGTGGTGGT CCACATACAC ACCACTGAGA CATGGAGTGT ACAATCAGAT AAAGAAGTTT
    CTCATATTCT ACTCGGCGCT TCATTTCTTG GTTCTCTACA CATATCAAAT TCCGATCGTT
    CACCACTCAT GGCTGCCGAC CGGTTCATTT CTGCCACGTC TGTTCGGACT TACTGTACTC
    ATGGATTCAT CATGTCCCGA ATGGTGGAAG TTTCCATTTG TAGCTCCAGA CTTTAACGAT
    GATGATTTGA TTATGAAATG GCCGTTGTAT GCAAATCCGA TTGTTGTACT GGTGTTCTTC
    TACCTCACAG TTGCTCAATA CAAATTCACG AGAAACGGAT CTCGTGAATA TATCGATGAT
    AACGAATATG GGTCATCAGT TCACGAAGAA CTTCTGTCCA ATGCATCCTC CTCTGCTAAT
    GATGATGAAC AGGGTCGAGC TCGTTCGCGA TCTCCGCTGC GAAATGGTGA AGAACAAGGC
    TCAATCCCAC TACGGAAAGT TACTTCGCAA GTTGTTGATA GGAATAAGTT GAGCAACATT
    TTTAATACTA CAGCACCCGG AGATAAGGAA TCTGCTGCTT CGAAAGGAAT GATTGCAGTC
    ATGACATTTG TCATTTTCCA TTCGTATTCA ATCGCGCTGA CTGCTATGAT GACATGGGCT
    CTCCTCTATC ATTCGATATT CGGTCTCATT CTCCTCATCC TCACATGTAT CTTGTGGATC
    TTCCGTGATA CGAGAAAGTC ATCGTTTGCA ATGGCCCCTA TTATTTTGAT GTATATCGAG
    TTTCTATTGA TTCTTCAATA TTTCCTCAGT ATGGATATTC ATGCAGAAAT TGGCGATCCA
    GCGTGGATGA ACTTTGTTGG AATCGAATGG ACTACTCTTC CAGTACATGC TGTCATCATT
    CTCTGTGTCC AGACTCTTCT CACTCTCCCA GTGTTCTTGC TTCTACGTTT GGCTCGCAGG
    GAGAAGTTTT ACGAATCGCT GAGTGATTAT GAACGACAAC GACGAATCAA CTCATATGGA
    ACATTTGGAG CATCAAAGAC TGGAGCTGGG GGTGTTGCAG TGGCTAAGTT TCAGGATCCA
    AAAAGTCGGA AGTTCGCGGC ATTTGTGGAA TATCTGTCGA ATAAAGTGTC TGTCTACTTC
    ATATTTGTAG TATCAGTGGT ACTTTTGGTG GTCTCTACGT GTTTTGCTCC GAATTTCTAC
    AATATTCTAT TCTTCGCACT ATGGGCATTG AACTTGATTT ATCTCAAGTT CTCATTCCGT
    CTGTATCGTG GATTGGCATA TGCATTCTGG CTAACTCTCA CGTTCTACAC TTCAATTGTT
    ATTATCGCCC TCTACATTTA TCAGTTCCCT GGAGTATCTC AATGGATTAT TCGAAATACT
    AGTTTGTCGC AGGAGTGGCT GAATGCAATT GGACTTGTCG ATTTTAGAGC TATCGGTGAA
    TCGGGAGCCC TATTTCTACA ATTACTCGCC CCAATTGCCT TGTTCGTCGT CACAATGCTC
    CAACTCAAGT TTTTCCATGG ACCATGGAGT CGAGCAACGT CACCAAGAAG AGCAGAAAAT
    GATCCACCTA CAAGTACGAC GGAGGCTGCG GCGGTAGCAA GCACATCTGG AACACAAGGA
    AGAGCACATG CTGCAGGCGA TACCCTTGTC AAGAAGCTGC ACAAATTGGC AAATCAGACA
    ATCGAACTGT TATGGAGGTT TTTCGAAGTT CACATTTCGA AAATTGTCTT TGTCATCATT
    GCGATTTTTA TTGCCAACAA TATCAATGCT CTATACATTC CACTTGTTAT TCTCCTCTCA
    TTGGCTATAT GTCTACCATC AGCTGCAGAT GGGATATTCT CTTTATTTAT GTGTGCCTAC
    TTATTCCTCG TGGCACTGAG CAAGATGATC TACCAACTGG ATATTGTTCC CGAGTTATCA
    CAAATCGACC GAGGAGTTGG AGCAGATAAC TGTTCACATG GAAATATTTC AATGCCTGAA
    TGGTTTGGAT TGAAGAAGGA AGTTGAAGGA ACTGAACCAA TTTATATGCT CTTTGGAGTC
    ATTGTTTCGA TTATTGCATT GGCTTTTCAA TCAATTGTTA TATATCGACA AAGACATTAT
    CGAGCTTCAC TGGGACTTCC TGAATCGATG CGAGCTAAAG TGTTCCCGGA CTTCCATCAT
    TCTCATTTTG ATAGATCTCT CAAAAATGCC ATCCAATTCT TGATAGACTA TGGTTTCTAT
    AAGTTTGGAC TTGAAATCAC GATGATCGCC ATTGGAATTG ACATTTTCAA TCGAATGGAT
    GCATTGGCAG CGATTCAATG TTTCTGGCTT GTCCTGTTTG CGCTCAATAA AAGAGTCTTT
    GTTCGAAGAA TATGGGTATT CTATGTGATC TATATGGCGA TTCTGTATCC ACTTCAGTTC
    TTTTCATATG TTGGATTACC ACCTGACAGT TGTATTGAGT ATCCATGGTC GTATTGGATT
    CCATCGTATA GTGATGATGC GAGATTCAAT CTGTCGTATC TTCTGAACTT GTCAATATAC
    GGTGTAAACT GGCCATCAGC ATATCTCATT GGCGATTTCT TCGTACTCCT CTTGGCATCA
    TGCCAATTAG CAGTTTTCCG GCGAGAAGGA GAAGATAATG ATTCAATTTA CAATGATGGA
    AACTTTGTGA TTAAGCCAGA AAATCCACAA TATGACTTTA TTGACACCAA AAAGAGTTAC
    GTTGATTATT TCAAATCATT CGTTTTCCAT TATGGACACT GGATTACATT GATGTCTACA
    CTAGCAGCAG GAATTGCTGG AACGTCACTT TTTGCTTTGG GATACATTAT ATTCACTCTG
    ACAATGCTGT GGTCTGGTAA CAATTTGTAT GTGATGAACT CAACTCTACG ATCATTTGAA
    CATACTTTGA AACGATGGAA TGCACTTTTG GGATACACAT TGTTCACGAT TACAATGAAA
    GTTTGCCTTC AAATATTTGG ATGTGTATTC CTGTCGTGGT TCGATCAATC TGGTGGATGG
    GGAAAGACTC TGTGCATCGT TCGTCAACTC TTCAGTATCA CATGTGTTAA TAACGAATGT
    CATGTGCTCA AAGAGCTGGA GGATTTCTCG AAGGCGTGTG CTGTTGAGAC GAAAGAAGGA
    AATATTGGAT TTGATGTGAT TGCGCTATCC TTCCTCGTCT TCCAAATCCG AATTTTCCAT
    TCTTGGTATT TCCAACACTG CATGGTTGAA TATCGAAGTG AGGTTATTCT GGCCAATAGA
    GGTGCCGTGC TGAAGAATCA ACTGATTGAA AAGGAAATGA AGGAACAAAA CGAGCAGCAA
    AAAGCCAAGT TCAATGATAT CAGAAGAAGA ACTGAAGCTA TTCGTGAAAG ATATCAAAAA
    CAAATCGAAC GAGGAGCTGC TGAACGTGAC TTCGAGCCGG TTACTTATGG GCACGCCAAA
    CGAGCGGGAG ACTACTACAT GTTCAAATAC GATCCTGAAA ATGATGATCT TGTGGAACCC
    GTCGACAGTT TTGTACCAGA AGTTGATCCC AAGGCAACTG CATATGATCG ACTTGATCCT
    GGACAGATAA TGTACGCTGC CACCGCTCAC GACCTCGACC TCGCCAAAAC TGTACAGCAA
    GTCAAGAAAG GCGATACTAT CAAAGATCCT GATTCACGTG CTCTTATCGC TGTTTCTGAA
    CCAGAAGCTC GTAAGCCAGG AGGCACTGAA GAAACGGATG GTGATGAAGA TGAGGATAAT
    AAGGATTCGA AAGTGGAAAG TACTGCTAAA TTCATTCAAA AAATGATCGC TTCTGCCTTG
    GACTTGTGTA GTGTAACACT AAACAAACTG TGCAGAGAAC ACCGATATGT TGGATTTGTT
    TTGAGCAAGG AAAAACAAAA GTTGAAGTCA GGACACTCGG AATCTCTTTC AAATACATCT
    AGAAAGCTGA CGGATATTCG ATCTGCAGTT GATCTACCAA GTCTTCAACT TGTGCAATCG
    GCGAATGATG TTGAAAAGAT GGAGACAGCC GTCAGCGTGG ATTGGCAACA AAAATCATCA
    GCAACGAGAC TATTGAATGC AGTTGTGAAT TGTATTGGAG CACACACTGA TATTCTCTGC
    TACTTCTTCG CAATTATGAC ACAAGTGATG ACTGGTGGAC TTATTACTCT TCCACTTCCT
    CTGATGTCAC TATTCTGGGG AAATCTATCG AATCCTCGTC CATCCAAGTT CTTCTGGGTC
    ACAATGATCA CCTACACTGA GTGTGTCATT GTTATCAAGT TTGTTTGCCA ATTTGCATTC
    ATGCCTTACA ATTCTATCAC GTGGCGAACA GAGCATCAAA TGGACCCGAT GTCATTGGAT
    AAGTTATTCG GAGTATCACA GAGAGATAGT TTTGCACTTT GGGACATTGT TTTGCTCTTC
    TCACTCTTCT TCCATCGGTA TATGCTCAGA AAGTTAGGGT TATGGAAGGA TGCCAATTTG
    ACGGACACGT TCACTTTGAA GGAAGAGCCT CGATCGGCGT CAGGATCGGA CACAGGATCA
    CCCAAGAAAA TTGCTCAAGA GCCAAAGGTT GTTGTAACTC AGTCAGATAC CCTTGAAGGT
    ACAAGTGGAG GAGAGATAGT CATACCATCT GATCCAAATG CAGTTTCCAA TATGGAAGAA
    TTAGACTGCG AACCTCCGAT TCCAGAGAAG CAAAGTGGAC CAATTGGAAG ATTCATTCAT
    CAATTATTCC ATCCAAAATT CCGTTACATT CGTGATTTGT ATCCAATAAT GTTTGGAATC
    GATGTGATAT GCTTCCTCAT CATGACATTT GGATACTCGG CATTTGGTGA AGGAGGTTCT
    GGAAATGTTT TAGATGATGT GAAGGCTTCT CGTATTCCGG TAACACTTGT TGTTATGCTC
    GTTGGAATGA CTCTGGCAAT TATCATTGAT CGAGCACTCT ACCTCAGAAA ATCAGTTGTC
    GGAAAGCTAA TCTATCAAGT CTTAATGATT GCTTTCCTTC ACATTTGGGT ATTCCTTGTT
    CTTCCAAATA TGACTCGTCG TTCTGCGATA TCGAATCACG TTGCTCAAGC ATTGTACGTC
    ATCAAATCGT GCTATTTCCT TGTGTCTGCT TGGCAGATCC GAAATGGTTA TCCTGAACTT
    TGTATTGGAA ATCTATTAAC ACATTCATAC GGAATGACCA ATATGATTGC ATTCAAAGTA
    TTCATGAATA TTCCATTCCT CTTCGAGCTT CGTACAGCTA TTGATTGGAC ATGGACAGAT
    ACTTCAATGC CTCTTTTCGA TTTCTTCAAT ATGGAAAATT TCTATGCTCA TATATTCAAT
    ATCAAGTGTG CCCGCCAGTT CGAAGCTGCC TATCCAGCTC CAAGAGGGAT TCCCAAAGGA
    AAGTTGGTCA AGTATATGAT GGGATTCCCA ATTATCATTG GAGTCGTTAT CTTCATCTTT
    TCTCCTCTAT TGCTCTGGTC GCTACTCAAT CAAATCGGAA CTATCAGTAT GCCAGAAAAA
    GTTACGTTGA GGATTTCGAT TGAAGGATAT CCACCACTTT ACGAGATGGA GGCACAAGGA
    AGTAATCACG ATAACGCTGA ACTTGGAATG ATAAAACCTG ATCAATTGGC ATCTTTGAAT
    CAAGCATTGA CGGATAGTTA CACGACACGA GACACTAATT CTATTTTAAG ATCAAGAATG
    TCTGTATCAT ATCTGAAAGG ATACACATAT GAGGATATTC TGATTGTGCG ATTCCGTCCA
    GAATCTGAAA TTTATTGGCC GATTTCACAG GATTCGAGAA ATGCGATGAT CGATAAATTG
    AGCCGAAACA CGTCAGTCAA TTTTGAAGTA TCTCTGGAAT TCACTCGGCC ATATGATCCA
    AATGAAAACG CTGCTTTAAA ACATTCGAAA TCGTGGCTTG TTCCAATCTC CCTGGATATG
    ACGATTCGAG CAAAAATTCA AAGTGCTCTC CGAGGAGATC CAGGTCATCC GATTCTCATT
    CCACAGTCAA TTCCTGCGTT TATTCAAGTT CCAAATCAAG GAGAATTGAC CCTTCCAACA
    TCAATCGGAA ATACTATTAT CAATGACGGA AATCCACGGA TTAATACGAC CGGGATGGAA
    AAATCAGACG AAGCTCGGGC TTGGTTCGAC TCGTTAACCT TGAATCTCGA ACAAGGAAAA
    TCGCAAAACG AGAAAATGTG GATCGCCACA TCCGAACATC CAGGAGACCA AAATGCGAAA
    CTCTGGATAA AGACTGCAAA TACGACGTAT TCGGGAAGGC CGTACCTTCA AGTTGTCGGA
    TTTATTGATA GAGCATTCCC ATCATTTTTG GCGAAAGTTT TCAAAGGAGG AGTCATTGCA
    GTGTATCTTT CAGTTATCCT CGTCGTTGGA AGAGGTCTGG TTCGTGGAAT CTTCACCACA
    TCTCCAAGCA CTGTCATGTT CACAGAATTA CCGAATGCGG ATCATCTTCT CAAAATTTGT
    CTCGACATCT ATTTGGTTCG AGAAGCGAAG GACTTCATGT TGGAGCAGGA TCTCTTCGCC
    AAATTAATAT TCCTGTTCCG ATCACCAGCG ACACTCATCG AATGGACACG AATGAGCAAA
    AAGAAGCAAG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.