daf-18 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	daf-18
Entrez Gene ID	176869
Full Name	Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_067525.5	NP_499926.1	Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18
NM_067525.6	NP_499926.1	Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our results imply that DAF-18/PTEN, through PAR-4/LKB1, can activate AAK-1/AMPK in the absence of apparent energy stress. As all components are conserved, similar signalling cascades may regulate stem cell activities in other organisms and be widely implicated in cancer.
Title: DAF-18/PTEN signals through AAK-1/AMPK to inhibit MPK-1/MAPK in feedback control of germline stem cell proliferation.

differential regulation of germline stem cell proliferation rates in Caenorhabditis elegans adults is accomplished through localized neutralization of insulin/IGF-1 signalling, requiring DAF-18/PTEN.
Title: DAF-18/PTEN locally antagonizes insulin signalling to couple germline stem cell proliferation to oocyte needs in C. elegans.

Doxycyclin ameliorates a starvation-induced germline tumor in C. elegans with a daf-18/PTEN mutant background.
Title: Doxycyclin ameliorates a starvation-induced germline tumor in C. elegans daf-18/PTEN mutant background.

Mutations in the PTEN (DAF-18) tumor suppressor gene may result in the simultaneous hyperactivation of two oncogenic signaling pathways.
Title: PTEN negatively regulates MAPK signaling during Caenorhabditis elegans vulval development.

DAF-18 modulates the PI3K signaling pathway to activate DAF-16/FOXO and promote developmental neurite outgrowth.
Title: A conserved PTEN/FOXO pathway regulates neuronal morphology during C. elegans development.

The following daf-18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the daf-18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa344146	NM_067525.6 Latest version!	Caenorhabditis elegans Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18 (daf-18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa344146	NM_067525.5	Caenorhabditis elegans Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18 (daf-18), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

daf-18 ( NM_067525.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499926.1 Caenorhabditis elegans Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18 (daf-18), partial mRNA.

Price & Availability↑

CloneID	OCa344146
Clone ID Related Accession (Same CDS sequence)	NM_067525.5 , NM_067525.6
Accession Version	NM_067525.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_067525.4. COMPLETENESS: incomplete on both ends.

ATGGTTACTC CTCCTCCAGA TGTGCCAAGC ACATCGACCA GGTCGATGGC TCGTGACCTT 
CAAGAGAATC CAAACCGACA ACCTGGTGAA CCACGTGTGT CTGAACCGTA TCACAATTCA 
ATCGTCGAGC GGATTCGCCA TATTTTTCGG ACGGCTGTAT CTTCCAATCG TTGTCGCACC 
GAGTACCAAA ATATCGACCT AGATTGTGCA TATATCACAG ACCGAATCAT AGCTATCGGT 
TATCCAGCAA CAGGAATCGA AGCGAATTTC CGTAACTCAA AAGTTCAAAC TCAACAATTT 
CTGACCAGGC GGCACGGAAA GGGCAACGTG AAGGTGTTTA ACCTGCGCGG TGGATACTAC 
TACGATGCGG ATAACTTCGA TGGAAATGTT ATTTGCTTCG ATATGACTGA TCATCATCCG 
CCGAGTCTCG AATTAATGGC TCCGTTTTGC AGAGAGGCTA AGGAATGGCT TGAAGCAGAC 
GATAAACATG TAATAGCTGT ACACTGTAAA GCTGGAAAAG GCCGTACCGG AGTGATGATA 
TGTGCTCTTC TCATCTACAT CAACTTCTAT CCGAGCCCAC GACAAATTCT CGACTACTAC 
TCAATAATTC GTACAAAAAA CAACAAAGGT GTCACAATTC CATCACAACG ACGCTACATT 
TACTACTACC ATAAGCTTCG TGAACGTGAG CTCAACTATT TACCATTGAG AATGCAGTTG 
ATTGGTGTCT ACGTGGAACG GCCTCCAAAG ACATGGGGTG GTGGTTCAAA GATAAAAGTG 
GAGGTTGGAA ATGGCTCGAC AATTTTATTT AAGCCGGATC CTCTCATAAT CTCCAAATCA 
AATCATCAGC GAGAGCGTGC GACGTGGCTG AACAACTGTG ATACGCCTAA CGAATTCGAC 
ACCGGAGAGC AAAAATATCA TGGATTTGTT TCCAAGAGAG CATACTGTTT TATGGTGCCA 
GAAGATGCTC CAGTATTTGT CGAAGGAGAT GTTCGTATAG ACATTCGCGA AATCGGATTT 
CTCAAAAAGT TTTCGGACGG GAAGATTGGT CATGTTTGGT TCAATACAAT GTTCGCATGT 
GATGGAGGAC TCAACGGTGG ACATTTCGAG TACGTAGACA AAACTCAGCC GTACATCGGA 
GACGATACAT CAATCGGACG GAAAAATGGA ATGCGAAGAA ATGAAACGCC GATGCGAAAA 
ATTGATCCAG AAACTGGAAA TGAATTTGAG TCTCCGTGGC AAATAGTGAA TCCTCCTGGA 
CTGGAAAAAC ATATTACGGA GGAACAAGCA ATGGAAAATT ATACCAATTA TGGCATGATT 
CCTCCTCGAT ACACGATCAG CAAGATTCTT CACGAAAAGC ATGAAAAAGG TATCGTCAAG 
GATGACTATA ATGATCGTAA GCTGCCAATG GGAGACAAAT CATACACGGA ATCAGGAAAA 
AGTGGAGATA TTCGAGGAGT CGGTGGTCCA TTTGAGATAC CATATAAAGC TGAGGAACAT 
GTTCTCACAT TTCCAGTTTA TGAAATGGAT CGAGCATTGA AGAGTAAAGA TCTTAACAAC 
GGAATGAAAC TTCACGTTGT TCTTCGTTGT GTAGATACTC GTGATTCAAA AATGATGGAA 
AAGAGCGAAG TGTTCGGCAA TCTGGCATTC CATAATGAAT CGACACGGAG GCTTCAAGCG 
TTGACTCAAA TGAATCCAAA ATGGCGACCT GAACCGTGTG CGTTCGGATC CAAAGGTGCT 
GAAATGCATT ACCCTCCGTC GGTTCGATAT TCAAGCAATG ATGGAAAGTA TAATGGAGCC 
TGCAGTGAGA ACCTTGTTAG CGATTTTTTC GAGCACAGAA ATATTGCCGT TCTTAATCGA 
TATTGCCGAT ATTTCTACAA GCAACGCAGT ACATCTCGAA GCCGTTATCC AAGAAAATTC 
AGATACTGTC CTCTGATCAA GAAACATTTC TACATTCCAG CTGATACCGA TGATGTTGAT 
GAAAATGGGC AACCGTTCTT CCACTCACCA GAGCATTACA TTAAAGAACA GGAAAAAATA 
GACGCAGAGA AAGCAGCTAA AGGAATTGAA AATACTGGAC CCAGTACTTC AGGATCAAGT 
GCTCCCGGAA CTATCAAGAA AACGGAAGCT TCACAATCCG ACAAGGTGAA GCCGGCAACT 
GAAGACGAAC TTCCTCCTGC GAGGCTACCG GATAATGTGC GAAGATTTCC AGTCGTCGGC 
GTTGATTTCG AAAATCCGGA AGAAGAATCG TGTGAACACA AAACCGTAGA GTCAATAGCT 
GGTTTTGAAC CACTCGAACA TCTATTCCAT GAATCATACC ATCCAAATAC GGCCGGTAAC 
ATGCTGCGTC AGGATTATCA CACTGATTCG GAAGTGAAAA TAGCTGAACA AGAGGCAAAA 
GCCTTCGTTG ACCAGTTGCT TAATGGACAA GGTGTATTAC AAGAGTTTAT GAAGCAATTC 
AAAGTACCAT CGGACAATTC CTTTGCTGAT TATGTAACCG GACAGGCCGA AGTTTTTAAA 
GCACAGATTG CGTTACTGGA GCAGTCGGAG GATTTTCAAC GAGTTCAAGC GAATGCAGAG 
GAAGTCGATC TTGAACACAC TCTTGGTGAA GCGTTTGAGC GATTCGGGCA CGTTGTAGAA 
GAATCGAATG GTTCTTCTAA AAATCCAAAA GCCCTGAAAA CTCGAGAACA AATGGTGAAA 
GAAACTGGCA AAGACACTCA GAAGACCCGC AATCATGTGC TTCTACATTT GGAAGCTAAT 
CATCGTGTGC AAATCGAGCG TCGTGAAACG TGCCCGGAGC TACATCCAGA GGATAAAATC 
CCAAGAATTG CTCATTTTTC CGAAAACAGC TTCTCGGATT CGAATTTTGA TCAAGCTATT 
TATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499926.1
CDS	1..2889
Translation	MVTPPPDVPSTSTRSMARDLQENPNRQPGEPRVSEPYHNSIVERIRHIFRTAVSSNRCRTEYQNIDLDCAYITDRIIAIGYPATGIEANFRNSKVQTQQFLTRRHGKGNVKVFNLRGGYYYDADNFDGNVICFDMTDHHPPSLELMAPFCREAKEWLEADDKHVIAVHCKAGKGRTGVMICALLIYINFYPSPRQILDYYSIIRTKNNKGVTIPSQRRYIYYYHKLRERELNYLPLRMQLIGVYVERPPKTWGGGSKIKVEVGNGSTILFKPDPLIISKSNHQRERATWLNNCDTPNEFDTGEQKYHGFVSKRAYCFMVPEDAPVFVEGDVRIDIREIGFLKKFSDGKIGHVWFNTMFACDGGLNGGHFEYVDKTQPYIGDDTSIGRKNGMRRNETPMRKIDPETGNEFESPWQIVNPPGLEKHITEEQAMENYTNYGMIPPRYTISKILHEKHEKGIVKDDYNDRKLPMGDKSYTESGKSGDIRGVGGPFEIPYKAEEHVLTFPVYEMDRALKSKDLNNGMKLHVVLRCVDTRDSKMMEKSEVFGNLAFHNESTRRLQALTQMNPKWRPEPCAFGSKGAEMHYPPSVRYSSNDGKYNGACSENLVSDFFEHRNIAVLNRYCRYFYKQRSTSRSRYPRKFRYCPLIKKHFYIPADTDDVDENGQPFFHSPEHYIKEQEKIDAEKAAKGIENTGPSTSGSSAPGTIKKTEASQSDKVKPATEDELPPARLPDNVRRFPVVGVDFENPEEESCEHKTVESIAGFEPLEHLFHESYHPNTAGNMLRQDYHTDSEVKIAEQEAKAFVDQLLNGQGVLQEFMKQFKVPSDNSFADYVTGQAEVFKAQIALLEQSEDFQRVQANAEEVDLEHTLGEAFERFGHVVEESNGSSKNPKALKTREQMVKETGKDTQKTRNHVLLHLEANHRVQIERRETCPELHPEDKIPRIAHFSENSFSDSNFDQAIYL

Target ORF information:

RefSeq Version	NM_067525.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18 (daf-18), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_067525.5

ORF Insert Sequence:

ATGGTTACTC CTCCTCCAGA TGTGCCAAGC ACATCGACCA GGTCGATGGC TCGTGACCTT 
CAAGAGAATC CAAACCGACA ACCTGGTGAA CCACGTGTGT CTGAACCGTA TCACAATTCA 
ATCGTCGAGC GGATTCGCCA TATTTTTCGG ACGGCTGTAT CTTCCAATCG TTGTCGCACC 
GAGTACCAAA ATATCGACCT AGATTGTGCA TATATCACAG ACCGAATCAT AGCTATCGGT 
TATCCAGCAA CAGGAATCGA AGCGAATTTC CGTAACTCAA AAGTTCAAAC TCAACAATTT 
CTGACCAGGC GGCACGGAAA GGGCAACGTG AAGGTGTTTA ACCTGCGCGG TGGATACTAC 
TACGATGCGG ATAACTTCGA TGGAAATGTT ATTTGCTTCG ATATGACTGA TCATCATCCG 
CCGAGTCTCG AATTAATGGC TCCGTTTTGC AGAGAGGCTA AGGAATGGCT TGAAGCAGAC 
GATAAACATG TAATAGCTGT ACACTGTAAA GCTGGAAAAG GCCGTACCGG AGTGATGATA 
TGTGCTCTTC TCATCTACAT CAACTTCTAT CCGAGCCCAC GACAAATTCT CGACTACTAC 
TCAATAATTC GTACAAAAAA CAACAAAGGT GTCACAATTC CATCACAACG ACGCTACATT 
TACTACTACC ATAAGCTTCG TGAACGTGAG CTCAACTATT TACCATTGAG AATGCAGTTG 
ATTGGTGTCT ACGTGGAACG GCCTCCAAAG ACATGGGGTG GTGGTTCAAA GATAAAAGTG 
GAGGTTGGAA ATGGCTCGAC AATTTTATTT AAGCCGGATC CTCTCATAAT CTCCAAATCA 
AATCATCAGC GAGAGCGTGC GACGTGGCTG AACAACTGTG ATACGCCTAA CGAATTCGAC 
ACCGGAGAGC AAAAATATCA TGGATTTGTT TCCAAGAGAG CATACTGTTT TATGGTGCCA 
GAAGATGCTC CAGTATTTGT CGAAGGAGAT GTTCGTATAG ACATTCGCGA AATCGGATTT 
CTCAAAAAGT TTTCGGACGG GAAGATTGGT CATGTTTGGT TCAATACAAT GTTCGCATGT 
GATGGAGGAC TCAACGGTGG ACATTTCGAG TACGTAGACA AAACTCAGCC GTACATCGGA 
GACGATACAT CAATCGGACG GAAAAATGGA ATGCGAAGAA ATGAAACGCC GATGCGAAAA 
ATTGATCCAG AAACTGGAAA TGAATTTGAG TCTCCGTGGC AAATAGTGAA TCCTCCTGGA 
CTGGAAAAAC ATATTACGGA GGAACAAGCA ATGGAAAATT ATACCAATTA TGGCATGATT 
CCTCCTCGAT ACACGATCAG CAAGATTCTT CACGAAAAGC ATGAAAAAGG TATCGTCAAG 
GATGACTATA ATGATCGTAA GCTGCCAATG GGAGACAAAT CATACACGGA ATCAGGAAAA 
AGTGGAGATA TTCGAGGAGT CGGTGGTCCA TTTGAGATAC CATATAAAGC TGAGGAACAT 
GTTCTCACAT TTCCAGTTTA TGAAATGGAT CGAGCATTGA AGAGTAAAGA TCTTAACAAC 
GGAATGAAAC TTCACGTTGT TCTTCGTTGT GTAGATACTC GTGATTCAAA AATGATGGAA 
AAGAGCGAAG TGTTCGGCAA TCTGGCATTC CATAATGAAT CGACACGGAG GCTTCAAGCG 
TTGACTCAAA TGAATCCAAA ATGGCGACCT GAACCGTGTG CGTTCGGATC CAAAGGTGCT 
GAAATGCATT ACCCTCCGTC GGTTCGATAT TCAAGCAATG ATGGAAAGTA TAATGGAGCC 
TGCAGTGAGA ACCTTGTTAG CGATTTTTTC GAGCACAGAA ATATTGCCGT TCTTAATCGA 
TATTGCCGAT ATTTCTACAA GCAACGCAGT ACATCTCGAA GCCGTTATCC AAGAAAATTC 
AGATACTGTC CTCTGATCAA GAAACATTTC TACATTCCAG CTGATACCGA TGATGTTGAT 
GAAAATGGGC AACCGTTCTT CCACTCACCA GAGCATTACA TTAAAGAACA GGAAAAAATA 
GACGCAGAGA AAGCAGCTAA AGGAATTGAA AATACTGGAC CCAGTACTTC AGGATCAAGT 
GCTCCCGGAA CTATCAAGAA AACGGAAGCT TCACAATCCG ACAAGGTGAA GCCGGCAACT 
GAAGACGAAC TTCCTCCTGC GAGGCTACCG GATAATGTGC GAAGATTTCC AGTCGTCGGC 
GTTGATTTCG AAAATCCGGA AGAAGAATCG TGTGAACACA AAACCGTAGA GTCAATAGCT 
GGTTTTGAAC CACTCGAACA TCTATTCCAT GAATCATACC ATCCAAATAC GGCCGGTAAC 
ATGCTGCGTC AGGATTATCA CACTGATTCG GAAGTGAAAA TAGCTGAACA AGAGGCAAAA 
GCCTTCGTTG ACCAGTTGCT TAATGGACAA GGTGTATTAC AAGAGTTTAT GAAGCAATTC 
AAAGTACCAT CGGACAATTC CTTTGCTGAT TATGTAACCG GACAGGCCGA AGTTTTTAAA 
GCACAGATTG CGTTACTGGA GCAGTCGGAG GATTTTCAAC GAGTTCAAGC GAATGCAGAG 
GAAGTCGATC TTGAACACAC TCTTGGTGAA GCGTTTGAGC GATTCGGGCA CGTTGTAGAA 
GAATCGAATG GTTCTTCTAA AAATCCAAAA GCCCTGAAAA CTCGAGAACA AATGGTGAAA 
GAAACTGGCA AAGACACTCA GAAGACCCGC AATCATGTGC TTCTACATTT GGAAGCTAAT 
CATCGTGTGC AAATCGAGCG TCGTGAAACG TGCCCGGAGC TACATCCAGA GGATAAAATC 
CCAAGAATTG CTCATTTTTC CGAAAACAGC TTCTCGGATT CGAATTTTGA TCAAGCTATT 
TATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

daf-18 ( NM_067525.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499926.1 Caenorhabditis elegans Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18 (daf-18), mRNA.

Price & Availability↑

CloneID	OCa344146
Clone ID Related Accession (Same CDS sequence)	NM_067525.5 , NM_067525.6
Accession Version	NM_067525.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_067525.5.

ATGGTTACTC CTCCTCCAGA TGTGCCAAGC ACATCGACCA GGTCGATGGC TCGTGACCTT 
CAAGAGAATC CAAACCGACA ACCTGGTGAA CCACGTGTGT CTGAACCGTA TCACAATTCA 
ATCGTCGAGC GGATTCGCCA TATTTTTCGG ACGGCTGTAT CTTCCAATCG TTGTCGCACC 
GAGTACCAAA ATATCGACCT AGATTGTGCA TATATCACAG ACCGAATCAT AGCTATCGGT 
TATCCAGCAA CAGGAATCGA AGCGAATTTC CGTAACTCAA AAGTTCAAAC TCAACAATTT 
CTGACCAGGC GGCACGGAAA GGGCAACGTG AAGGTGTTTA ACCTGCGCGG TGGATACTAC 
TACGATGCGG ATAACTTCGA TGGAAATGTT ATTTGCTTCG ATATGACTGA TCATCATCCG 
CCGAGTCTCG AATTAATGGC TCCGTTTTGC AGAGAGGCTA AGGAATGGCT TGAAGCAGAC 
GATAAACATG TAATAGCTGT ACACTGTAAA GCTGGAAAAG GCCGTACCGG AGTGATGATA 
TGTGCTCTTC TCATCTACAT CAACTTCTAT CCGAGCCCAC GACAAATTCT CGACTACTAC 
TCAATAATTC GTACAAAAAA CAACAAAGGT GTCACAATTC CATCACAACG ACGCTACATT 
TACTACTACC ATAAGCTTCG TGAACGTGAG CTCAACTATT TACCATTGAG AATGCAGTTG 
ATTGGTGTCT ACGTGGAACG GCCTCCAAAG ACATGGGGTG GTGGTTCAAA GATAAAAGTG 
GAGGTTGGAA ATGGCTCGAC AATTTTATTT AAGCCGGATC CTCTCATAAT CTCCAAATCA 
AATCATCAGC GAGAGCGTGC GACGTGGCTG AACAACTGTG ATACGCCTAA CGAATTCGAC 
ACCGGAGAGC AAAAATATCA TGGATTTGTT TCCAAGAGAG CATACTGTTT TATGGTGCCA 
GAAGATGCTC CAGTATTTGT CGAAGGAGAT GTTCGTATAG ACATTCGCGA AATCGGATTT 
CTCAAAAAGT TTTCGGACGG GAAGATTGGT CATGTTTGGT TCAATACAAT GTTCGCATGT 
GATGGAGGAC TCAACGGTGG ACATTTCGAG TACGTAGACA AAACTCAGCC GTACATCGGA 
GACGATACAT CAATCGGACG GAAAAATGGA ATGCGAAGAA ATGAAACGCC GATGCGAAAA 
ATTGATCCAG AAACTGGAAA TGAATTTGAG TCTCCGTGGC AAATAGTGAA TCCTCCTGGA 
CTGGAAAAAC ATATTACGGA GGAACAAGCA ATGGAAAATT ATACCAATTA TGGCATGATT 
CCTCCTCGAT ACACGATCAG CAAGATTCTT CACGAAAAGC ATGAAAAAGG TATCGTCAAG 
GATGACTATA ATGATCGTAA GCTGCCAATG GGAGACAAAT CATACACGGA ATCAGGAAAA 
AGTGGAGATA TTCGAGGAGT CGGTGGTCCA TTTGAGATAC CATATAAAGC TGAGGAACAT 
GTTCTCACAT TTCCAGTTTA TGAAATGGAT CGAGCATTGA AGAGTAAAGA TCTTAACAAC 
GGAATGAAAC TTCACGTTGT TCTTCGTTGT GTAGATACTC GTGATTCAAA AATGATGGAA 
AAGAGCGAAG TGTTCGGCAA TCTGGCATTC CATAATGAAT CGACACGGAG GCTTCAAGCG 
TTGACTCAAA TGAATCCAAA ATGGCGACCT GAACCGTGTG CGTTCGGATC CAAAGGTGCT 
GAAATGCATT ACCCTCCGTC GGTTCGATAT TCAAGCAATG ATGGAAAGTA TAATGGAGCC 
TGCAGTGAGA ACCTTGTTAG CGATTTTTTC GAGCACAGAA ATATTGCCGT TCTTAATCGA 
TATTGCCGAT ATTTCTACAA GCAACGCAGT ACATCTCGAA GCCGTTATCC AAGAAAATTC 
AGATACTGTC CTCTGATCAA GAAACATTTC TACATTCCAG CTGATACCGA TGATGTTGAT 
GAAAATGGGC AACCGTTCTT CCACTCACCA GAGCATTACA TTAAAGAACA GGAAAAAATA 
GACGCAGAGA AAGCAGCTAA AGGAATTGAA AATACTGGAC CCAGTACTTC AGGATCAAGT 
GCTCCCGGAA CTATCAAGAA AACGGAAGCT TCACAATCCG ACAAGGTGAA GCCGGCAACT 
GAAGACGAAC TTCCTCCTGC GAGGCTACCG GATAATGTGC GAAGATTTCC AGTCGTCGGC 
GTTGATTTCG AAAATCCGGA AGAAGAATCG TGTGAACACA AAACCGTAGA GTCAATAGCT 
GGTTTTGAAC CACTCGAACA TCTATTCCAT GAATCATACC ATCCAAATAC GGCCGGTAAC 
ATGCTGCGTC AGGATTATCA CACTGATTCG GAAGTGAAAA TAGCTGAACA AGAGGCAAAA 
GCCTTCGTTG ACCAGTTGCT TAATGGACAA GGTGTATTAC AAGAGTTTAT GAAGCAATTC 
AAAGTACCAT CGGACAATTC CTTTGCTGAT TATGTAACCG GACAGGCCGA AGTTTTTAAA 
GCACAGATTG CGTTACTGGA GCAGTCGGAG GATTTTCAAC GAGTTCAAGC GAATGCAGAG 
GAAGTCGATC TTGAACACAC TCTTGGTGAA GCGTTTGAGC GATTCGGGCA CGTTGTAGAA 
GAATCGAATG GTTCTTCTAA AAATCCAAAA GCCCTGAAAA CTCGAGAACA AATGGTGAAA 
GAAACTGGCA AAGACACTCA GAAGACCCGC AATCATGTGC TTCTACATTT GGAAGCTAAT 
CATCGTGTGC AAATCGAGCG TCGTGAAACG TGCCCGGAGC TACATCCAGA GGATAAAATC 
CCAAGAATTG CTCATTTTTC CGAAAACAGC TTCTCGGATT CGAATTTTGA TCAAGCTATT 
TATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499926.1
CDS	34..2922
Translation	MVTPPPDVPSTSTRSMARDLQENPNRQPGEPRVSEPYHNSIVERIRHIFRTAVSSNRCRTEYQNIDLDCAYITDRIIAIGYPATGIEANFRNSKVQTQQFLTRRHGKGNVKVFNLRGGYYYDADNFDGNVICFDMTDHHPPSLELMAPFCREAKEWLEADDKHVIAVHCKAGKGRTGVMICALLIYINFYPSPRQILDYYSIIRTKNNKGVTIPSQRRYIYYYHKLRERELNYLPLRMQLIGVYVERPPKTWGGGSKIKVEVGNGSTILFKPDPLIISKSNHQRERATWLNNCDTPNEFDTGEQKYHGFVSKRAYCFMVPEDAPVFVEGDVRIDIREIGFLKKFSDGKIGHVWFNTMFACDGGLNGGHFEYVDKTQPYIGDDTSIGRKNGMRRNETPMRKIDPETGNEFESPWQIVNPPGLEKHITEEQAMENYTNYGMIPPRYTISKILHEKHEKGIVKDDYNDRKLPMGDKSYTESGKSGDIRGVGGPFEIPYKAEEHVLTFPVYEMDRALKSKDLNNGMKLHVVLRCVDTRDSKMMEKSEVFGNLAFHNESTRRLQALTQMNPKWRPEPCAFGSKGAEMHYPPSVRYSSNDGKYNGACSENLVSDFFEHRNIAVLNRYCRYFYKQRSTSRSRYPRKFRYCPLIKKHFYIPADTDDVDENGQPFFHSPEHYIKEQEKIDAEKAAKGIENTGPSTSGSSAPGTIKKTEASQSDKVKPATEDELPPARLPDNVRRFPVVGVDFENPEEESCEHKTVESIAGFEPLEHLFHESYHPNTAGNMLRQDYHTDSEVKIAEQEAKAFVDQLLNGQGVLQEFMKQFKVPSDNSFADYVTGQAEVFKAQIALLEQSEDFQRVQANAEEVDLEHTLGEAFERFGHVVEESNGSSKNPKALKTREQMVKETGKDTQKTRNHVLLHLEANHRVQIERRETCPELHPEDKIPRIAHFSENSFSDSNFDQAIYL

Target ORF information:

RefSeq Version	NM_067525.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18 (daf-18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_067525.6

ORF Insert Sequence:

ATGGTTACTC CTCCTCCAGA TGTGCCAAGC ACATCGACCA GGTCGATGGC TCGTGACCTT 
CAAGAGAATC CAAACCGACA ACCTGGTGAA CCACGTGTGT CTGAACCGTA TCACAATTCA 
ATCGTCGAGC GGATTCGCCA TATTTTTCGG ACGGCTGTAT CTTCCAATCG TTGTCGCACC 
GAGTACCAAA ATATCGACCT AGATTGTGCA TATATCACAG ACCGAATCAT AGCTATCGGT 
TATCCAGCAA CAGGAATCGA AGCGAATTTC CGTAACTCAA AAGTTCAAAC TCAACAATTT 
CTGACCAGGC GGCACGGAAA GGGCAACGTG AAGGTGTTTA ACCTGCGCGG TGGATACTAC 
TACGATGCGG ATAACTTCGA TGGAAATGTT ATTTGCTTCG ATATGACTGA TCATCATCCG 
CCGAGTCTCG AATTAATGGC TCCGTTTTGC AGAGAGGCTA AGGAATGGCT TGAAGCAGAC 
GATAAACATG TAATAGCTGT ACACTGTAAA GCTGGAAAAG GCCGTACCGG AGTGATGATA 
TGTGCTCTTC TCATCTACAT CAACTTCTAT CCGAGCCCAC GACAAATTCT CGACTACTAC 
TCAATAATTC GTACAAAAAA CAACAAAGGT GTCACAATTC CATCACAACG ACGCTACATT 
TACTACTACC ATAAGCTTCG TGAACGTGAG CTCAACTATT TACCATTGAG AATGCAGTTG 
ATTGGTGTCT ACGTGGAACG GCCTCCAAAG ACATGGGGTG GTGGTTCAAA GATAAAAGTG 
GAGGTTGGAA ATGGCTCGAC AATTTTATTT AAGCCGGATC CTCTCATAAT CTCCAAATCA 
AATCATCAGC GAGAGCGTGC GACGTGGCTG AACAACTGTG ATACGCCTAA CGAATTCGAC 
ACCGGAGAGC AAAAATATCA TGGATTTGTT TCCAAGAGAG CATACTGTTT TATGGTGCCA 
GAAGATGCTC CAGTATTTGT CGAAGGAGAT GTTCGTATAG ACATTCGCGA AATCGGATTT 
CTCAAAAAGT TTTCGGACGG GAAGATTGGT CATGTTTGGT TCAATACAAT GTTCGCATGT 
GATGGAGGAC TCAACGGTGG ACATTTCGAG TACGTAGACA AAACTCAGCC GTACATCGGA 
GACGATACAT CAATCGGACG GAAAAATGGA ATGCGAAGAA ATGAAACGCC GATGCGAAAA 
ATTGATCCAG AAACTGGAAA TGAATTTGAG TCTCCGTGGC AAATAGTGAA TCCTCCTGGA 
CTGGAAAAAC ATATTACGGA GGAACAAGCA ATGGAAAATT ATACCAATTA TGGCATGATT 
CCTCCTCGAT ACACGATCAG CAAGATTCTT CACGAAAAGC ATGAAAAAGG TATCGTCAAG 
GATGACTATA ATGATCGTAA GCTGCCAATG GGAGACAAAT CATACACGGA ATCAGGAAAA 
AGTGGAGATA TTCGAGGAGT CGGTGGTCCA TTTGAGATAC CATATAAAGC TGAGGAACAT 
GTTCTCACAT TTCCAGTTTA TGAAATGGAT CGAGCATTGA AGAGTAAAGA TCTTAACAAC 
GGAATGAAAC TTCACGTTGT TCTTCGTTGT GTAGATACTC GTGATTCAAA AATGATGGAA 
AAGAGCGAAG TGTTCGGCAA TCTGGCATTC CATAATGAAT CGACACGGAG GCTTCAAGCG 
TTGACTCAAA TGAATCCAAA ATGGCGACCT GAACCGTGTG CGTTCGGATC CAAAGGTGCT 
GAAATGCATT ACCCTCCGTC GGTTCGATAT TCAAGCAATG ATGGAAAGTA TAATGGAGCC 
TGCAGTGAGA ACCTTGTTAG CGATTTTTTC GAGCACAGAA ATATTGCCGT TCTTAATCGA 
TATTGCCGAT ATTTCTACAA GCAACGCAGT ACATCTCGAA GCCGTTATCC AAGAAAATTC 
AGATACTGTC CTCTGATCAA GAAACATTTC TACATTCCAG CTGATACCGA TGATGTTGAT 
GAAAATGGGC AACCGTTCTT CCACTCACCA GAGCATTACA TTAAAGAACA GGAAAAAATA 
GACGCAGAGA AAGCAGCTAA AGGAATTGAA AATACTGGAC CCAGTACTTC AGGATCAAGT 
GCTCCCGGAA CTATCAAGAA AACGGAAGCT TCACAATCCG ACAAGGTGAA GCCGGCAACT 
GAAGACGAAC TTCCTCCTGC GAGGCTACCG GATAATGTGC GAAGATTTCC AGTCGTCGGC 
GTTGATTTCG AAAATCCGGA AGAAGAATCG TGTGAACACA AAACCGTAGA GTCAATAGCT 
GGTTTTGAAC CACTCGAACA TCTATTCCAT GAATCATACC ATCCAAATAC GGCCGGTAAC 
ATGCTGCGTC AGGATTATCA CACTGATTCG GAAGTGAAAA TAGCTGAACA AGAGGCAAAA 
GCCTTCGTTG ACCAGTTGCT TAATGGACAA GGTGTATTAC AAGAGTTTAT GAAGCAATTC 
AAAGTACCAT CGGACAATTC CTTTGCTGAT TATGTAACCG GACAGGCCGA AGTTTTTAAA 
GCACAGATTG CGTTACTGGA GCAGTCGGAG GATTTTCAAC GAGTTCAAGC GAATGCAGAG 
GAAGTCGATC TTGAACACAC TCTTGGTGAA GCGTTTGAGC GATTCGGGCA CGTTGTAGAA 
GAATCGAATG GTTCTTCTAA AAATCCAAAA GCCCTGAAAA CTCGAGAACA AATGGTGAAA 
GAAACTGGCA AAGACACTCA GAAGACCCGC AATCATGTGC TTCTACATTT GGAAGCTAAT 
CATCGTGTGC AAATCGAGCG TCGTGAAACG TGCCCGGAGC TACATCCAGA GGATAAAATC 
CCAAGAATTG CTCATTTTTC CGAAAACAGC TTCTCGGATT CGAATTTTGA TCAAGCTATT 
TATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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daf-18 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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