nfi-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	nfi-1
Entrez Gene ID	174225
Full Name	NFI (Nuclear Factor I) family
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001027334.2	NP_001022505.1	NFI (Nuclear Factor I) family
NM_001027334.3	NP_001022505.1	CTF/NF-I domain-containing protein
NM_001027335.4	NP_001022506.1	CTF/NF-I domain-containing protein
NM_001027336.3	NP_001022507.1	NFI (Nuclear Factor I) family
NM_001027335.3	NP_001022506.1	NFI (Nuclear Factor I) family
NM_001027336.4	NP_001022507.1	CTF/NF-I domain-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These experiments provide a foundation for understanding how NFI-1 is recruited to unexpectedly few in vivo sites to perform its developmental functions, despite a vast over-representation of its binding motif.
Title: DNA-binding specificity and in vivo targets of Caenorhabditis elegans nuclear factor I.

We propose some relationships between the pharyngeal pumping and lifespan phenotypes and potential mechanisms of nfi-1 function
Title: Lifespan extension and increased pumping rate accompany pharyngeal muscle-specific expression of nfi-1 in C. elegans.

The phenotype of nfi-1 null worms indicates that NFI functions in multiple developmental and behavioral systems in C. elegans, likely regulating genes that function in motility, egg-laying, pharyngeal pumping and lifespan maintenance.
Title: nfi-I affects behavior and life-span in C. elegans but is not essential for DNA replication or survival.

The following nfi-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nfi-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa338794	NM_001027334.3 Latest version!	Caenorhabditis elegans CTF/NF-I domain-containing protein (nfi-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa338795	NM_001027335.4 Latest version!	Caenorhabditis elegans CTF/NF-I domain-containing protein (nfi-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa338796	NM_001027336.4 Latest version!	Caenorhabditis elegans CTF/NF-I domain-containing protein (nfi-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OCa338794	NM_001027334.2	Caenorhabditis elegans NFI (Nuclear Factor I) family (nfi-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa338795	NM_001027335.3	Caenorhabditis elegans NFI (Nuclear Factor I) family (nfi-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa338796	NM_001027336.3	Caenorhabditis elegans NFI (Nuclear Factor I) family (nfi-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nfi-1 ( NM_001027334.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022505.1 Caenorhabditis elegans NFI (Nuclear Factor I) family (nfi-1), partial mRNA.

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CloneID	OCa338794
Clone ID Related Accession (Same CDS sequence)	NM_001027334.2 , NM_001027334.3
Accession Version	NM_001027334.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	NFI (Nuclear Factor I) family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_001027334.1. COMPLETENESS: incomplete on both ends.

ATGGAGCCGC ATCTCAAGAT CGATGTCAGC TCCGCCAGCG GTTCGACGAC CACTGGTGCA 
ACAGCTTCAA CATCAGAAGC ACCTCAAGAC TCACAGGCAC AGCAGACAAT GCCTCCACCA 
TCTTCTGATT GGAGTAATCA ATTCAATTCT CCAGAAGCCG AAGATATGGG ACCATTTGTC 
GAACAACTTC TACCTTTTGT CAGAGCAAGT GCCTATAATT GGTTTCATCT GCAGGCAGCA 
AAACGACGGC ACTTCAAAGA ATTTGATAAG AAGATGTGTG CGAGTGAAGA AAACGCGAAA 
TTAGCAGAAT TACAAAATGA TAGAGATGAA TTAAAAGTGA AATGGGCTTC CAGACTTTTG 
GGAAAAATAA AAAAAGATAT TCAAAATGAC GATAAAGAAG CGTTTATTTC GGCTATCAAT 
GGAAGTGAGC CGAACAAGTG TATTATATCA GTTGCTGATC AGAAAGGGAA AATGCGGCGA 
ATTGATTGCT TACGCCAAGC TGACAAAGTT TGGAGGCTTG ATCTGGTTAC TATTATTTTA 
TTTAAAGGAA TTCCTCTCGA GAGCACCGAC GGCGAGCGTC TCGAACGATC CGAGGCATGT 
GTCCACCCTC TGTGCATAAA TCCTTTTCAT ATGGCGATTA GTGTTCGCGG TCTTGACGTT 
TTTATGGCAA ACTATCTGAA AGACGTTGAC ACTAAAATTA CATTGACCTA TCCGAGAAAT 
GATGAACTTT CTGATACAGT GATGGTAAAG CAAGAGCCCG GTGAACAGAC AATTGTAGCT 
CCTCATGCAG TTCTTGGCAC AAGTTCTACT CATACTCGTG TCTGGGAAAC GAATTCCGAA 
CGAAATGCGG AAACAATCAT TATCACTTAT GATCCACAGA AAGCTTGCCA TACTTATTTT 
GGTGGTCGTG CTACTCTTGC CCAACAGTCT CTTTCTGCGG GAAACACGTA TATGGTTAAC 
AAAACCGCAG TCGACAACAA CTTTTTCAAT GCAAAAAGAT CTGTTCTATG TCTACCACCT 
CCTCCGATTC AAAATTGTTT TCCTTATCCC ATCGCGGGAA CATCTGATTC TCAACAAATG 
GATATGTCGG AGGATTCCAA CGATGGACCT TCAGAGAAAC GAAGTCGGGA TATATCATCG 
CATGATTCTC CTAATTCCAG CACTAACGAC GAAGTTCGGC GGATAGTGGA AAGTGGAACG 
GAAAAATTGG TACTCGGAAG CTCCATATGG GCAGCACCAG GCCAATTCTC TCGTACACAA 
CAAAATCAAG GAGCTCCCGG AACGAGTCGG CAAGTGAGAC CGCTTCCCGA TTTTCAAAGT 
CAAGATTCTG CGAGGAGTCC GGGTGCATTC CGTTCAACAG CAAAACCCGT TTGTCGAATG 
ACAGTGAATA CTGGAAATCA TGGCGATGTT GGAGTCGTTG TTGTTGATGA AAGAAATCGG 
GAGCATGTGA TTCATGCTCA ACATATAGTG AATGCATTGA GCTCACTTCG AACGACACCA 
TCGATGCGAG AAAGTCCAGT TGGCAGAAAA AGAATGCATC CGCATACGAG CAATTCATTT 
GAATTTTTGA ATTGCAATCA GGAAATGAAT AAAAATGAAG GAGCTTTAGG AAGCGATATT 
TCACCGACTC ACACTGCTGT TTCTAATCTA ATCAGCAGAG AAAGTAGTGG ATACATGGCA 
AGCCCAACAA AATTCACAAC AGCACGCGGA GATACAACAA GCTTCAGCAA AATTTTCCAA 
AAGATCGAAG AGAAACATCT GCAACATAAT CAACCGAGCA CGAGCTACTG TAATTCACAA 
ATCCAACCAC CAATCTTATC ATCGAAACCA GTTGATTCAT CAGTGAAATT AATTGCTCCA 
GTTGCAGTGA AACCAATTAT GTCAGGATGT AATTCAATCA TTCCATCACC CATCACAACT 
CCTCGTATAA CTCCTTCATT TCGAATGCTT GAAGATGATT CATTGATTAA TGTACTTGGA 
CAACTTGCAC ACAGTAATGA CGGAACAACT TTAAACGATA GCTTCATTCA ACATCTTATT 
GATACCAATT CTCGATCTCC ATTGCTTAGT AGTGGAAATG CTTTTTCGGC ATTATCAATG 
GGAGCTGTGA GTGGATTAGT TCCTGGTAAT TCGATTCATC GTCCGGATAG TTCTGCAAGC 
AACGGATCGA ATTCGTTGGG TGTAGCAATG GGATTGGCCG TTCCTCAAAA TATTGCACTG 
GCCGTCCAGC AAACTCAAAA TGCAATGTCA CCTCTTCATC AAATTCGTGT TAGCGTTGGA 
GCTCCACCAG CATGCTCCCC GTCATCATCA AATTCATCTC TAGGAGCTGC AAATCAAGCA 
CCGGTCTCAA ATACTCCACA AGATCCGAAT GCTCCAAAGC TGCCAACTGA CTTTTCACAT 
GCTCTTCGTA ATGAGAAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022505.1
CDS	1..2424
Translation	MEPHLKIDVSSASGSTTTGATASTSEAPQDSQAQQTMPPPSSDWSNQFNSPEAEDMGPFVEQLLPFVRASAYNWFHLQAAKRRHFKEFDKKMCASEENAKLAELQNDRDELKVKWASRLLGKIKKDIQNDDKEAFISAINGSEPNKCIISVADQKGKMRRIDCLRQADKVWRLDLVTIILFKGIPLESTDGERLERSEACVHPLCINPFHMAISVRGLDVFMANYLKDVDTKITLTYPRNDELSDTVMVKQEPGEQTIVAPHAVLGTSSTHTRVWETNSERNAETIIITYDPQKACHTYFGGRATLAQQSLSAGNTYMVNKTAVDNNFFNAKRSVLCLPPPPIQNCFPYPIAGTSDSQQMDMSEDSNDGPSEKRSRDISSHDSPNSSTNDEVRRIVESGTEKLVLGSSIWAAPGQFSRTQQNQGAPGTSRQVRPLPDFQSQDSARSPGAFRSTAKPVCRMTVNTGNHGDVGVVVVDERNREHVIHAQHIVNALSSLRTTPSMRESPVGRKRMHPHTSNSFEFLNCNQEMNKNEGALGSDISPTHTAVSNLISRESSGYMASPTKFTTARGDTTSFSKIFQKIEEKHLQHNQPSTSYCNSQIQPPILSSKPVDSSVKLIAPVAVKPIMSGCNSIIPSPITTPRITPSFRMLEDDSLINVLGQLAHSNDGTTLNDSFIQHLIDTNSRSPLLSSGNAFSALSMGAVSGLVPGNSIHRPDSSASNGSNSLGVAMGLAVPQNIALAVQQTQNAMSPLHQIRVSVGAPPACSPSSSNSSLGAANQAPVSNTPQDPNAPKLPTDFSHALRNEKK

Target ORF information:

RefSeq Version	NM_001027334.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans NFI (Nuclear Factor I) family (nfi-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027334.2

ORF Insert Sequence:

ATGGAGCCGC ATCTCAAGAT CGATGTCAGC TCCGCCAGCG GTTCGACGAC CACTGGTGCA 
ACAGCTTCAA CATCAGAAGC ACCTCAAGAC TCACAGGCAC AGCAGACAAT GCCTCCACCA 
TCTTCTGATT GGAGTAATCA ATTCAATTCT CCAGAAGCCG AAGATATGGG ACCATTTGTC 
GAACAACTTC TACCTTTTGT CAGAGCAAGT GCCTATAATT GGTTTCATCT GCAGGCAGCA 
AAACGACGGC ACTTCAAAGA ATTTGATAAG AAGATGTGTG CGAGTGAAGA AAACGCGAAA 
TTAGCAGAAT TACAAAATGA TAGAGATGAA TTAAAAGTGA AATGGGCTTC CAGACTTTTG 
GGAAAAATAA AAAAAGATAT TCAAAATGAC GATAAAGAAG CGTTTATTTC GGCTATCAAT 
GGAAGTGAGC CGAACAAGTG TATTATATCA GTTGCTGATC AGAAAGGGAA AATGCGGCGA 
ATTGATTGCT TACGCCAAGC TGACAAAGTT TGGAGGCTTG ATCTGGTTAC TATTATTTTA 
TTTAAAGGAA TTCCTCTCGA GAGCACCGAC GGCGAGCGTC TCGAACGATC CGAGGCATGT 
GTCCACCCTC TGTGCATAAA TCCTTTTCAT ATGGCGATTA GTGTTCGCGG TCTTGACGTT 
TTTATGGCAA ACTATCTGAA AGACGTTGAC ACTAAAATTA CATTGACCTA TCCGAGAAAT 
GATGAACTTT CTGATACAGT GATGGTAAAG CAAGAGCCCG GTGAACAGAC AATTGTAGCT 
CCTCATGCAG TTCTTGGCAC AAGTTCTACT CATACTCGTG TCTGGGAAAC GAATTCCGAA 
CGAAATGCGG AAACAATCAT TATCACTTAT GATCCACAGA AAGCTTGCCA TACTTATTTT 
GGTGGTCGTG CTACTCTTGC CCAACAGTCT CTTTCTGCGG GAAACACGTA TATGGTTAAC 
AAAACCGCAG TCGACAACAA CTTTTTCAAT GCAAAAAGAT CTGTTCTATG TCTACCACCT 
CCTCCGATTC AAAATTGTTT TCCTTATCCC ATCGCGGGAA CATCTGATTC TCAACAAATG 
GATATGTCGG AGGATTCCAA CGATGGACCT TCAGAGAAAC GAAGTCGGGA TATATCATCG 
CATGATTCTC CTAATTCCAG CACTAACGAC GAAGTTCGGC GGATAGTGGA AAGTGGAACG 
GAAAAATTGG TACTCGGAAG CTCCATATGG GCAGCACCAG GCCAATTCTC TCGTACACAA 
CAAAATCAAG GAGCTCCCGG AACGAGTCGG CAAGTGAGAC CGCTTCCCGA TTTTCAAAGT 
CAAGATTCTG CGAGGAGTCC GGGTGCATTC CGTTCAACAG CAAAACCCGT TTGTCGAATG 
ACAGTGAATA CTGGAAATCA TGGCGATGTT GGAGTCGTTG TTGTTGATGA AAGAAATCGG 
GAGCATGTGA TTCATGCTCA ACATATAGTG AATGCATTGA GCTCACTTCG AACGACACCA 
TCGATGCGAG AAAGTCCAGT TGGCAGAAAA AGAATGCATC CGCATACGAG CAATTCATTT 
GAATTTTTGA ATTGCAATCA GGAAATGAAT AAAAATGAAG GAGCTTTAGG AAGCGATATT 
TCACCGACTC ACACTGCTGT TTCTAATCTA ATCAGCAGAG AAAGTAGTGG ATACATGGCA 
AGCCCAACAA AATTCACAAC AGCACGCGGA GATACAACAA GCTTCAGCAA AATTTTCCAA 
AAGATCGAAG AGAAACATCT GCAACATAAT CAACCGAGCA CGAGCTACTG TAATTCACAA 
ATCCAACCAC CAATCTTATC ATCGAAACCA GTTGATTCAT CAGTGAAATT AATTGCTCCA 
GTTGCAGTGA AACCAATTAT GTCAGGATGT AATTCAATCA TTCCATCACC CATCACAACT 
CCTCGTATAA CTCCTTCATT TCGAATGCTT GAAGATGATT CATTGATTAA TGTACTTGGA 
CAACTTGCAC ACAGTAATGA CGGAACAACT TTAAACGATA GCTTCATTCA ACATCTTATT 
GATACCAATT CTCGATCTCC ATTGCTTAGT AGTGGAAATG CTTTTTCGGC ATTATCAATG 
GGAGCTGTGA GTGGATTAGT TCCTGGTAAT TCGATTCATC GTCCGGATAG TTCTGCAAGC 
AACGGATCGA ATTCGTTGGG TGTAGCAATG GGATTGGCCG TTCCTCAAAA TATTGCACTG 
GCCGTCCAGC AAACTCAAAA TGCAATGTCA CCTCTTCATC AAATTCGTGT TAGCGTTGGA 
GCTCCACCAG CATGCTCCCC GTCATCATCA AATTCATCTC TAGGAGCTGC AAATCAAGCA 
CCGGTCTCAA ATACTCCACA AGATCCGAAT GCTCCAAAGC TGCCAACTGA CTTTTCACAT 
GCTCTTCGTA ATGAGAAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfi-1 ( NM_001027334.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022505.1 Caenorhabditis elegans CTF/NF-I domain-containing protein (nfi-1), mRNA.

Price & Availability↑

CloneID	OCa338794
Clone ID Related Accession (Same CDS sequence)	NM_001027334.2 , NM_001027334.3
Accession Version	NM_001027334.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	CTF/NF-I domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001027334.2.

ATGGAGCCGC ATCTCAAGAT CGATGTCAGC TCCGCCAGCG GTTCGACGAC CACTGGTGCA 
ACAGCTTCAA CATCAGAAGC ACCTCAAGAC TCACAGGCAC AGCAGACAAT GCCTCCACCA 
TCTTCTGATT GGAGTAATCA ATTCAATTCT CCAGAAGCCG AAGATATGGG ACCATTTGTC 
GAACAACTTC TACCTTTTGT CAGAGCAAGT GCCTATAATT GGTTTCATCT GCAGGCAGCA 
AAACGACGGC ACTTCAAAGA ATTTGATAAG AAGATGTGTG CGAGTGAAGA AAACGCGAAA 
TTAGCAGAAT TACAAAATGA TAGAGATGAA TTAAAAGTGA AATGGGCTTC CAGACTTTTG 
GGAAAAATAA AAAAAGATAT TCAAAATGAC GATAAAGAAG CGTTTATTTC GGCTATCAAT 
GGAAGTGAGC CGAACAAGTG TATTATATCA GTTGCTGATC AGAAAGGGAA AATGCGGCGA 
ATTGATTGCT TACGCCAAGC TGACAAAGTT TGGAGGCTTG ATCTGGTTAC TATTATTTTA 
TTTAAAGGAA TTCCTCTCGA GAGCACCGAC GGCGAGCGTC TCGAACGATC CGAGGCATGT 
GTCCACCCTC TGTGCATAAA TCCTTTTCAT ATGGCGATTA GTGTTCGCGG TCTTGACGTT 
TTTATGGCAA ACTATCTGAA AGACGTTGAC ACTAAAATTA CATTGACCTA TCCGAGAAAT 
GATGAACTTT CTGATACAGT GATGGTAAAG CAAGAGCCCG GTGAACAGAC AATTGTAGCT 
CCTCATGCAG TTCTTGGCAC AAGTTCTACT CATACTCGTG TCTGGGAAAC GAATTCCGAA 
CGAAATGCGG AAACAATCAT TATCACTTAT GATCCACAGA AAGCTTGCCA TACTTATTTT 
GGTGGTCGTG CTACTCTTGC CCAACAGTCT CTTTCTGCGG GAAACACGTA TATGGTTAAC 
AAAACCGCAG TCGACAACAA CTTTTTCAAT GCAAAAAGAT CTGTTCTATG TCTACCACCT 
CCTCCGATTC AAAATTGTTT TCCTTATCCC ATCGCGGGAA CATCTGATTC TCAACAAATG 
GATATGTCGG AGGATTCCAA CGATGGACCT TCAGAGAAAC GAAGTCGGGA TATATCATCG 
CATGATTCTC CTAATTCCAG CACTAACGAC GAAGTTCGGC GGATAGTGGA AAGTGGAACG 
GAAAAATTGG TACTCGGAAG CTCCATATGG GCAGCACCAG GCCAATTCTC TCGTACACAA 
CAAAATCAAG GAGCTCCCGG AACGAGTCGG CAAGTGAGAC CGCTTCCCGA TTTTCAAAGT 
CAAGATTCTG CGAGGAGTCC GGGTGCATTC CGTTCAACAG CAAAACCCGT TTGTCGAATG 
ACAGTGAATA CTGGAAATCA TGGCGATGTT GGAGTCGTTG TTGTTGATGA AAGAAATCGG 
GAGCATGTGA TTCATGCTCA ACATATAGTG AATGCATTGA GCTCACTTCG AACGACACCA 
TCGATGCGAG AAAGTCCAGT TGGCAGAAAA AGAATGCATC CGCATACGAG CAATTCATTT 
GAATTTTTGA ATTGCAATCA GGAAATGAAT AAAAATGAAG GAGCTTTAGG AAGCGATATT 
TCACCGACTC ACACTGCTGT TTCTAATCTA ATCAGCAGAG AAAGTAGTGG ATACATGGCA 
AGCCCAACAA AATTCACAAC AGCACGCGGA GATACAACAA GCTTCAGCAA AATTTTCCAA 
AAGATCGAAG AGAAACATCT GCAACATAAT CAACCGAGCA CGAGCTACTG TAATTCACAA 
ATCCAACCAC CAATCTTATC ATCGAAACCA GTTGATTCAT CAGTGAAATT AATTGCTCCA 
GTTGCAGTGA AACCAATTAT GTCAGGATGT AATTCAATCA TTCCATCACC CATCACAACT 
CCTCGTATAA CTCCTTCATT TCGAATGCTT GAAGATGATT CATTGATTAA TGTACTTGGA 
CAACTTGCAC ACAGTAATGA CGGAACAACT TTAAACGATA GCTTCATTCA ACATCTTATT 
GATACCAATT CTCGATCTCC ATTGCTTAGT AGTGGAAATG CTTTTTCGGC ATTATCAATG 
GGAGCTGTGA GTGGATTAGT TCCTGGTAAT TCGATTCATC GTCCGGATAG TTCTGCAAGC 
AACGGATCGA ATTCGTTGGG TGTAGCAATG GGATTGGCCG TTCCTCAAAA TATTGCACTG 
GCCGTCCAGC AAACTCAAAA TGCAATGTCA CCTCTTCATC AAATTCGTGT TAGCGTTGGA 
GCTCCACCAG CATGCTCCCC GTCATCATCA AATTCATCTC TAGGAGCTGC AAATCAAGCA 
CCGGTCTCAA ATACTCCACA AGATCCGAAT GCTCCAAAGC TGCCAACTGA CTTTTCACAT 
GCTCTTCGTA ATGAGAAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022505.1
CDS	101..2524
Translation	MEPHLKIDVSSASGSTTTGATASTSEAPQDSQAQQTMPPPSSDWSNQFNSPEAEDMGPFVEQLLPFVRASAYNWFHLQAAKRRHFKEFDKKMCASEENAKLAELQNDRDELKVKWASRLLGKIKKDIQNDDKEAFISAINGSEPNKCIISVADQKGKMRRIDCLRQADKVWRLDLVTIILFKGIPLESTDGERLERSEACVHPLCINPFHMAISVRGLDVFMANYLKDVDTKITLTYPRNDELSDTVMVKQEPGEQTIVAPHAVLGTSSTHTRVWETNSERNAETIIITYDPQKACHTYFGGRATLAQQSLSAGNTYMVNKTAVDNNFFNAKRSVLCLPPPPIQNCFPYPIAGTSDSQQMDMSEDSNDGPSEKRSRDISSHDSPNSSTNDEVRRIVESGTEKLVLGSSIWAAPGQFSRTQQNQGAPGTSRQVRPLPDFQSQDSARSPGAFRSTAKPVCRMTVNTGNHGDVGVVVVDERNREHVIHAQHIVNALSSLRTTPSMRESPVGRKRMHPHTSNSFEFLNCNQEMNKNEGALGSDISPTHTAVSNLISRESSGYMASPTKFTTARGDTTSFSKIFQKIEEKHLQHNQPSTSYCNSQIQPPILSSKPVDSSVKLIAPVAVKPIMSGCNSIIPSPITTPRITPSFRMLEDDSLINVLGQLAHSNDGTTLNDSFIQHLIDTNSRSPLLSSGNAFSALSMGAVSGLVPGNSIHRPDSSASNGSNSLGVAMGLAVPQNIALAVQQTQNAMSPLHQIRVSVGAPPACSPSSSNSSLGAANQAPVSNTPQDPNAPKLPTDFSHALRNEKK

Target ORF information:

RefSeq Version	NM_001027334.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans CTF/NF-I domain-containing protein (nfi-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027334.3

ORF Insert Sequence:

ATGGAGCCGC ATCTCAAGAT CGATGTCAGC TCCGCCAGCG GTTCGACGAC CACTGGTGCA 
ACAGCTTCAA CATCAGAAGC ACCTCAAGAC TCACAGGCAC AGCAGACAAT GCCTCCACCA 
TCTTCTGATT GGAGTAATCA ATTCAATTCT CCAGAAGCCG AAGATATGGG ACCATTTGTC 
GAACAACTTC TACCTTTTGT CAGAGCAAGT GCCTATAATT GGTTTCATCT GCAGGCAGCA 
AAACGACGGC ACTTCAAAGA ATTTGATAAG AAGATGTGTG CGAGTGAAGA AAACGCGAAA 
TTAGCAGAAT TACAAAATGA TAGAGATGAA TTAAAAGTGA AATGGGCTTC CAGACTTTTG 
GGAAAAATAA AAAAAGATAT TCAAAATGAC GATAAAGAAG CGTTTATTTC GGCTATCAAT 
GGAAGTGAGC CGAACAAGTG TATTATATCA GTTGCTGATC AGAAAGGGAA AATGCGGCGA 
ATTGATTGCT TACGCCAAGC TGACAAAGTT TGGAGGCTTG ATCTGGTTAC TATTATTTTA 
TTTAAAGGAA TTCCTCTCGA GAGCACCGAC GGCGAGCGTC TCGAACGATC CGAGGCATGT 
GTCCACCCTC TGTGCATAAA TCCTTTTCAT ATGGCGATTA GTGTTCGCGG TCTTGACGTT 
TTTATGGCAA ACTATCTGAA AGACGTTGAC ACTAAAATTA CATTGACCTA TCCGAGAAAT 
GATGAACTTT CTGATACAGT GATGGTAAAG CAAGAGCCCG GTGAACAGAC AATTGTAGCT 
CCTCATGCAG TTCTTGGCAC AAGTTCTACT CATACTCGTG TCTGGGAAAC GAATTCCGAA 
CGAAATGCGG AAACAATCAT TATCACTTAT GATCCACAGA AAGCTTGCCA TACTTATTTT 
GGTGGTCGTG CTACTCTTGC CCAACAGTCT CTTTCTGCGG GAAACACGTA TATGGTTAAC 
AAAACCGCAG TCGACAACAA CTTTTTCAAT GCAAAAAGAT CTGTTCTATG TCTACCACCT 
CCTCCGATTC AAAATTGTTT TCCTTATCCC ATCGCGGGAA CATCTGATTC TCAACAAATG 
GATATGTCGG AGGATTCCAA CGATGGACCT TCAGAGAAAC GAAGTCGGGA TATATCATCG 
CATGATTCTC CTAATTCCAG CACTAACGAC GAAGTTCGGC GGATAGTGGA AAGTGGAACG 
GAAAAATTGG TACTCGGAAG CTCCATATGG GCAGCACCAG GCCAATTCTC TCGTACACAA 
CAAAATCAAG GAGCTCCCGG AACGAGTCGG CAAGTGAGAC CGCTTCCCGA TTTTCAAAGT 
CAAGATTCTG CGAGGAGTCC GGGTGCATTC CGTTCAACAG CAAAACCCGT TTGTCGAATG 
ACAGTGAATA CTGGAAATCA TGGCGATGTT GGAGTCGTTG TTGTTGATGA AAGAAATCGG 
GAGCATGTGA TTCATGCTCA ACATATAGTG AATGCATTGA GCTCACTTCG AACGACACCA 
TCGATGCGAG AAAGTCCAGT TGGCAGAAAA AGAATGCATC CGCATACGAG CAATTCATTT 
GAATTTTTGA ATTGCAATCA GGAAATGAAT AAAAATGAAG GAGCTTTAGG AAGCGATATT 
TCACCGACTC ACACTGCTGT TTCTAATCTA ATCAGCAGAG AAAGTAGTGG ATACATGGCA 
AGCCCAACAA AATTCACAAC AGCACGCGGA GATACAACAA GCTTCAGCAA AATTTTCCAA 
AAGATCGAAG AGAAACATCT GCAACATAAT CAACCGAGCA CGAGCTACTG TAATTCACAA 
ATCCAACCAC CAATCTTATC ATCGAAACCA GTTGATTCAT CAGTGAAATT AATTGCTCCA 
GTTGCAGTGA AACCAATTAT GTCAGGATGT AATTCAATCA TTCCATCACC CATCACAACT 
CCTCGTATAA CTCCTTCATT TCGAATGCTT GAAGATGATT CATTGATTAA TGTACTTGGA 
CAACTTGCAC ACAGTAATGA CGGAACAACT TTAAACGATA GCTTCATTCA ACATCTTATT 
GATACCAATT CTCGATCTCC ATTGCTTAGT AGTGGAAATG CTTTTTCGGC ATTATCAATG 
GGAGCTGTGA GTGGATTAGT TCCTGGTAAT TCGATTCATC GTCCGGATAG TTCTGCAAGC 
AACGGATCGA ATTCGTTGGG TGTAGCAATG GGATTGGCCG TTCCTCAAAA TATTGCACTG 
GCCGTCCAGC AAACTCAAAA TGCAATGTCA CCTCTTCATC AAATTCGTGT TAGCGTTGGA 
GCTCCACCAG CATGCTCCCC GTCATCATCA AATTCATCTC TAGGAGCTGC AAATCAAGCA 
CCGGTCTCAA ATACTCCACA AGATCCGAAT GCTCCAAAGC TGCCAACTGA CTTTTCACAT 
GCTCTTCGTA ATGAGAAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfi-1 ( NM_001027335.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022506.1 Caenorhabditis elegans CTF/NF-I domain-containing protein (nfi-1), mRNA.

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CloneID	OCa338795
Clone ID Related Accession (Same CDS sequence)	NM_001027335.4 , NM_001027335.3
Accession Version	NM_001027335.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	CTF/NF-I domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001027335.3.

ATGCCTCCAC CATCTTCTGA TTGGAGTAAT CAATTCAATT CTCCAGAAGC CGTGAGTCCG 
AAAGCCAATG GTATCAAATG CTTCAGTCCT TACTCACAGG AAGATATGGG ACCATTTGTC 
GAACAACTTC TACCTTTTGT CAGAGCAAGT GCCTATAATT GGTTTCATCT GCAGGCAGCA 
AAACGACGGC ACTTCAAAGA ATTTGATAAG AAGATGTGTG CGAGTGAAGA AAACGCGAAA 
TTAGCAGAAT TACAAAATGA TAGAGATGAA TTAAAAGTGA AATGGGCTTC CAGACTTTTG 
GGAAAAATAA AAAAAGATAT TCAAAATGAC GATAAAGAAG CGTTTATTTC GGCTATCAAT 
GGAAGTGAGC CGAACAAGTG TATTATATCA GTTGCTGATC AGAAAGGGAA AATGCGGCGA 
ATTGATTGCT TACGCCAAGC TGACAAAGTT TGGAGGCTTG ATCTGGTTAC TATTATTTTA 
TTTAAAGGAA TTCCTCTCGA GAGCACCGAC GGCGAGCGTC TCGAACGATC CGAGGCATGT 
GTCCACCCTC TGTGCATAAA TCCTTTTCAT ATGGCGATTA GTGTTCGCGG TCTTGACGTT 
TTTATGGCAA ACTATCTGAA AGACGTTGAC ACTAAAATTA CATTGACCTA TCCGAGAAAT 
GATGAACTTT CTGATACAGT GATGGTAAAG CAAGAGCCCG GTGAACAGAC AATTGTAGCT 
CCTCATGCAG TTCTTGGCAC AAGTTCTACT CATACTCGTG TCTGGGAAAC GAATTCCGAA 
CGAAATGCGG AAACAATCAT TATCACTTAT GATCCACAGA AAGCTTGCCA TACTTATTTT 
GGTGGTCGTG CTACTCTTGC CCAACAGTCT CTTTCTGCGG GAAACACGTA TATGGTTAAC 
AAAACCGCAG TCGACAACAA CTTTTTCAAT GCAAAAAGAT CTGTTCTATG TCTACCACCT 
CCTCCGATTC AAAATTGTTT TCCTTATCCC ATCGCGGGAA CATCTGATTC TCAACAAATG 
GATATGTCGG AGGATTCCAA CGATGGACCT TCAGAGAAAC GAAGTCGGGA TATATCATCG 
CATGATTCTC CTAATTCCAG CACTAACGAC GAAGTTCGGC GGATAGTGGA AAGTGGAACG 
GAAAAATTGG TACTCGGAAG CTCCATATGG GCAGCACCAG GCCAATTCTC TCGTACACAA 
CAAAATCAAG GAGCTCCCGG AACGAGTCGG CAAGTGAGAC CGCTTCCCGA TTTTCAAAGT 
CAAGATTCTG CGAGGAGTCC GGGTGCATTC CGTTCAACAG CAAAACCCGT TTGTCGAATG 
ACAGTGAATA CTGGAAATCA TGGCGATGTT GGAGTCGTTG TTGTTGATGA AAGAAATCGG 
GAGCATGTGA TTCATGCTCA ACATATAGTG AATGCATTGA GCTCACTTCG AACGACACCA 
TCGATGCGAG AAAGTCCAGT TGGCAGAAAA AGAATGCATC CGCATACGAG CAATTCATTT 
GAATTTTTGA ATTGCAATCA GGAAATGAAT AAAAATGAAG GAGCTTTAGG AAGCGATATT 
TCACCGACTC ACACTGCTGT TTCTAATCTA ATCAGCAGAG AAAGTAGTGG ATACATGGCA 
AGCCCAACAA AATTCACAAC AGCACGCGGA GATACAACAA GCTTCAGCAA AATTTTCCAA 
AAGATCGAAG AGAAACATCT GCAACATAAT CAACCGAGCA CGAGCTACTG TAATTCACAA 
ATCCAACCAC CAATCTTATC ATCGAAACCA GTTGATTCAT CAGTGAAATT AATTGCTCCA 
GTTGCAGTGA AACCAATTAT GTCAGGATGT AATTCAATCA TTCCATCACC CATCACAACT 
CCTCGTATAA CTCCTTCATT TCGAATGCTT GAAGATGATT CATTGATTAA TGTACTTGGA 
CAACTTGCAC ACAGTAATGA CGGAACAACT TTAAACGATA GCTTCATTCA ACATCTTATT 
GATACCAATT CTCGATCTCC ATTGCTTAGT AGTGGAAATG CTTTTTCGGC ATTATCAATG 
GGAGCTGTGA GTGGATTAGT TCCTGGTAAT TCGATTCATC GTCCGGATAG TTCTGCAAGC 
AACGGATCGA ATTCGTTGGG TGTAGCAATG GGATTGGCCG TTCCTCAAAA TATTGCACTG 
GCCGTCCAGC AAACTCAAAA TGCAATGTCA CCTCTTCATC AAATTCGTGT TAGCGTTGGA 
GCTCCACCAG CATGCTCCCC GTCATCATCA AATTCATCTC TAGGAGCTGC AAATCAAGCA 
CCGGTCTCAA ATACTCCACA AGATCCGAAT GCTCCAAAGC TGCCAACTGA CTTTTCACAT 
GCTCTTCGTA ATGAGAAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022506.1
CDS	172..2535
Translation	MPPPSSDWSNQFNSPEAVSPKANGIKCFSPYSQEDMGPFVEQLLPFVRASAYNWFHLQAAKRRHFKEFDKKMCASEENAKLAELQNDRDELKVKWASRLLGKIKKDIQNDDKEAFISAINGSEPNKCIISVADQKGKMRRIDCLRQADKVWRLDLVTIILFKGIPLESTDGERLERSEACVHPLCINPFHMAISVRGLDVFMANYLKDVDTKITLTYPRNDELSDTVMVKQEPGEQTIVAPHAVLGTSSTHTRVWETNSERNAETIIITYDPQKACHTYFGGRATLAQQSLSAGNTYMVNKTAVDNNFFNAKRSVLCLPPPPIQNCFPYPIAGTSDSQQMDMSEDSNDGPSEKRSRDISSHDSPNSSTNDEVRRIVESGTEKLVLGSSIWAAPGQFSRTQQNQGAPGTSRQVRPLPDFQSQDSARSPGAFRSTAKPVCRMTVNTGNHGDVGVVVVDERNREHVIHAQHIVNALSSLRTTPSMRESPVGRKRMHPHTSNSFEFLNCNQEMNKNEGALGSDISPTHTAVSNLISRESSGYMASPTKFTTARGDTTSFSKIFQKIEEKHLQHNQPSTSYCNSQIQPPILSSKPVDSSVKLIAPVAVKPIMSGCNSIIPSPITTPRITPSFRMLEDDSLINVLGQLAHSNDGTTLNDSFIQHLIDTNSRSPLLSSGNAFSALSMGAVSGLVPGNSIHRPDSSASNGSNSLGVAMGLAVPQNIALAVQQTQNAMSPLHQIRVSVGAPPACSPSSSNSSLGAANQAPVSNTPQDPNAPKLPTDFSHALRNEKK

Target ORF information:

RefSeq Version	NM_001027335.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans CTF/NF-I domain-containing protein (nfi-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027335.4

ORF Insert Sequence:

ATGCCTCCAC CATCTTCTGA TTGGAGTAAT CAATTCAATT CTCCAGAAGC CGTGAGTCCG 
AAAGCCAATG GTATCAAATG CTTCAGTCCT TACTCACAGG AAGATATGGG ACCATTTGTC 
GAACAACTTC TACCTTTTGT CAGAGCAAGT GCCTATAATT GGTTTCATCT GCAGGCAGCA 
AAACGACGGC ACTTCAAAGA ATTTGATAAG AAGATGTGTG CGAGTGAAGA AAACGCGAAA 
TTAGCAGAAT TACAAAATGA TAGAGATGAA TTAAAAGTGA AATGGGCTTC CAGACTTTTG 
GGAAAAATAA AAAAAGATAT TCAAAATGAC GATAAAGAAG CGTTTATTTC GGCTATCAAT 
GGAAGTGAGC CGAACAAGTG TATTATATCA GTTGCTGATC AGAAAGGGAA AATGCGGCGA 
ATTGATTGCT TACGCCAAGC TGACAAAGTT TGGAGGCTTG ATCTGGTTAC TATTATTTTA 
TTTAAAGGAA TTCCTCTCGA GAGCACCGAC GGCGAGCGTC TCGAACGATC CGAGGCATGT 
GTCCACCCTC TGTGCATAAA TCCTTTTCAT ATGGCGATTA GTGTTCGCGG TCTTGACGTT 
TTTATGGCAA ACTATCTGAA AGACGTTGAC ACTAAAATTA CATTGACCTA TCCGAGAAAT 
GATGAACTTT CTGATACAGT GATGGTAAAG CAAGAGCCCG GTGAACAGAC AATTGTAGCT 
CCTCATGCAG TTCTTGGCAC AAGTTCTACT CATACTCGTG TCTGGGAAAC GAATTCCGAA 
CGAAATGCGG AAACAATCAT TATCACTTAT GATCCACAGA AAGCTTGCCA TACTTATTTT 
GGTGGTCGTG CTACTCTTGC CCAACAGTCT CTTTCTGCGG GAAACACGTA TATGGTTAAC 
AAAACCGCAG TCGACAACAA CTTTTTCAAT GCAAAAAGAT CTGTTCTATG TCTACCACCT 
CCTCCGATTC AAAATTGTTT TCCTTATCCC ATCGCGGGAA CATCTGATTC TCAACAAATG 
GATATGTCGG AGGATTCCAA CGATGGACCT TCAGAGAAAC GAAGTCGGGA TATATCATCG 
CATGATTCTC CTAATTCCAG CACTAACGAC GAAGTTCGGC GGATAGTGGA AAGTGGAACG 
GAAAAATTGG TACTCGGAAG CTCCATATGG GCAGCACCAG GCCAATTCTC TCGTACACAA 
CAAAATCAAG GAGCTCCCGG AACGAGTCGG CAAGTGAGAC CGCTTCCCGA TTTTCAAAGT 
CAAGATTCTG CGAGGAGTCC GGGTGCATTC CGTTCAACAG CAAAACCCGT TTGTCGAATG 
ACAGTGAATA CTGGAAATCA TGGCGATGTT GGAGTCGTTG TTGTTGATGA AAGAAATCGG 
GAGCATGTGA TTCATGCTCA ACATATAGTG AATGCATTGA GCTCACTTCG AACGACACCA 
TCGATGCGAG AAAGTCCAGT TGGCAGAAAA AGAATGCATC CGCATACGAG CAATTCATTT 
GAATTTTTGA ATTGCAATCA GGAAATGAAT AAAAATGAAG GAGCTTTAGG AAGCGATATT 
TCACCGACTC ACACTGCTGT TTCTAATCTA ATCAGCAGAG AAAGTAGTGG ATACATGGCA 
AGCCCAACAA AATTCACAAC AGCACGCGGA GATACAACAA GCTTCAGCAA AATTTTCCAA 
AAGATCGAAG AGAAACATCT GCAACATAAT CAACCGAGCA CGAGCTACTG TAATTCACAA 
ATCCAACCAC CAATCTTATC ATCGAAACCA GTTGATTCAT CAGTGAAATT AATTGCTCCA 
GTTGCAGTGA AACCAATTAT GTCAGGATGT AATTCAATCA TTCCATCACC CATCACAACT 
CCTCGTATAA CTCCTTCATT TCGAATGCTT GAAGATGATT CATTGATTAA TGTACTTGGA 
CAACTTGCAC ACAGTAATGA CGGAACAACT TTAAACGATA GCTTCATTCA ACATCTTATT 
GATACCAATT CTCGATCTCC ATTGCTTAGT AGTGGAAATG CTTTTTCGGC ATTATCAATG 
GGAGCTGTGA GTGGATTAGT TCCTGGTAAT TCGATTCATC GTCCGGATAG TTCTGCAAGC 
AACGGATCGA ATTCGTTGGG TGTAGCAATG GGATTGGCCG TTCCTCAAAA TATTGCACTG 
GCCGTCCAGC AAACTCAAAA TGCAATGTCA CCTCTTCATC AAATTCGTGT TAGCGTTGGA 
GCTCCACCAG CATGCTCCCC GTCATCATCA AATTCATCTC TAGGAGCTGC AAATCAAGCA 
CCGGTCTCAA ATACTCCACA AGATCCGAAT GCTCCAAAGC TGCCAACTGA CTTTTCACAT 
GCTCTTCGTA ATGAGAAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfi-1 ( NM_001027335.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022506.1 Caenorhabditis elegans NFI (Nuclear Factor I) family (nfi-1), partial mRNA.

Price & Availability↑

CloneID	OCa338795
Clone ID Related Accession (Same CDS sequence)	NM_001027335.4 , NM_001027335.3
Accession Version	NM_001027335.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	NFI (Nuclear Factor I) family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_001027335.2. COMPLETENESS: incomplete on both ends.

ATGCCTCCAC CATCTTCTGA TTGGAGTAAT CAATTCAATT CTCCAGAAGC CGTGAGTCCG 
AAAGCCAATG GTATCAAATG CTTCAGTCCT TACTCACAGG AAGATATGGG ACCATTTGTC 
GAACAACTTC TACCTTTTGT CAGAGCAAGT GCCTATAATT GGTTTCATCT GCAGGCAGCA 
AAACGACGGC ACTTCAAAGA ATTTGATAAG AAGATGTGTG CGAGTGAAGA AAACGCGAAA 
TTAGCAGAAT TACAAAATGA TAGAGATGAA TTAAAAGTGA AATGGGCTTC CAGACTTTTG 
GGAAAAATAA AAAAAGATAT TCAAAATGAC GATAAAGAAG CGTTTATTTC GGCTATCAAT 
GGAAGTGAGC CGAACAAGTG TATTATATCA GTTGCTGATC AGAAAGGGAA AATGCGGCGA 
ATTGATTGCT TACGCCAAGC TGACAAAGTT TGGAGGCTTG ATCTGGTTAC TATTATTTTA 
TTTAAAGGAA TTCCTCTCGA GAGCACCGAC GGCGAGCGTC TCGAACGATC CGAGGCATGT 
GTCCACCCTC TGTGCATAAA TCCTTTTCAT ATGGCGATTA GTGTTCGCGG TCTTGACGTT 
TTTATGGCAA ACTATCTGAA AGACGTTGAC ACTAAAATTA CATTGACCTA TCCGAGAAAT 
GATGAACTTT CTGATACAGT GATGGTAAAG CAAGAGCCCG GTGAACAGAC AATTGTAGCT 
CCTCATGCAG TTCTTGGCAC AAGTTCTACT CATACTCGTG TCTGGGAAAC GAATTCCGAA 
CGAAATGCGG AAACAATCAT TATCACTTAT GATCCACAGA AAGCTTGCCA TACTTATTTT 
GGTGGTCGTG CTACTCTTGC CCAACAGTCT CTTTCTGCGG GAAACACGTA TATGGTTAAC 
AAAACCGCAG TCGACAACAA CTTTTTCAAT GCAAAAAGAT CTGTTCTATG TCTACCACCT 
CCTCCGATTC AAAATTGTTT TCCTTATCCC ATCGCGGGAA CATCTGATTC TCAACAAATG 
GATATGTCGG AGGATTCCAA CGATGGACCT TCAGAGAAAC GAAGTCGGGA TATATCATCG 
CATGATTCTC CTAATTCCAG CACTAACGAC GAAGTTCGGC GGATAGTGGA AAGTGGAACG 
GAAAAATTGG TACTCGGAAG CTCCATATGG GCAGCACCAG GCCAATTCTC TCGTACACAA 
CAAAATCAAG GAGCTCCCGG AACGAGTCGG CAAGTGAGAC CGCTTCCCGA TTTTCAAAGT 
CAAGATTCTG CGAGGAGTCC GGGTGCATTC CGTTCAACAG CAAAACCCGT TTGTCGAATG 
ACAGTGAATA CTGGAAATCA TGGCGATGTT GGAGTCGTTG TTGTTGATGA AAGAAATCGG 
GAGCATGTGA TTCATGCTCA ACATATAGTG AATGCATTGA GCTCACTTCG AACGACACCA 
TCGATGCGAG AAAGTCCAGT TGGCAGAAAA AGAATGCATC CGCATACGAG CAATTCATTT 
GAATTTTTGA ATTGCAATCA GGAAATGAAT AAAAATGAAG GAGCTTTAGG AAGCGATATT 
TCACCGACTC ACACTGCTGT TTCTAATCTA ATCAGCAGAG AAAGTAGTGG ATACATGGCA 
AGCCCAACAA AATTCACAAC AGCACGCGGA GATACAACAA GCTTCAGCAA AATTTTCCAA 
AAGATCGAAG AGAAACATCT GCAACATAAT CAACCGAGCA CGAGCTACTG TAATTCACAA 
ATCCAACCAC CAATCTTATC ATCGAAACCA GTTGATTCAT CAGTGAAATT AATTGCTCCA 
GTTGCAGTGA AACCAATTAT GTCAGGATGT AATTCAATCA TTCCATCACC CATCACAACT 
CCTCGTATAA CTCCTTCATT TCGAATGCTT GAAGATGATT CATTGATTAA TGTACTTGGA 
CAACTTGCAC ACAGTAATGA CGGAACAACT TTAAACGATA GCTTCATTCA ACATCTTATT 
GATACCAATT CTCGATCTCC ATTGCTTAGT AGTGGAAATG CTTTTTCGGC ATTATCAATG 
GGAGCTGTGA GTGGATTAGT TCCTGGTAAT TCGATTCATC GTCCGGATAG TTCTGCAAGC 
AACGGATCGA ATTCGTTGGG TGTAGCAATG GGATTGGCCG TTCCTCAAAA TATTGCACTG 
GCCGTCCAGC AAACTCAAAA TGCAATGTCA CCTCTTCATC AAATTCGTGT TAGCGTTGGA 
GCTCCACCAG CATGCTCCCC GTCATCATCA AATTCATCTC TAGGAGCTGC AAATCAAGCA 
CCGGTCTCAA ATACTCCACA AGATCCGAAT GCTCCAAAGC TGCCAACTGA CTTTTCACAT 
GCTCTTCGTA ATGAGAAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022506.1
CDS	1..2364
Translation	MPPPSSDWSNQFNSPEAVSPKANGIKCFSPYSQEDMGPFVEQLLPFVRASAYNWFHLQAAKRRHFKEFDKKMCASEENAKLAELQNDRDELKVKWASRLLGKIKKDIQNDDKEAFISAINGSEPNKCIISVADQKGKMRRIDCLRQADKVWRLDLVTIILFKGIPLESTDGERLERSEACVHPLCINPFHMAISVRGLDVFMANYLKDVDTKITLTYPRNDELSDTVMVKQEPGEQTIVAPHAVLGTSSTHTRVWETNSERNAETIIITYDPQKACHTYFGGRATLAQQSLSAGNTYMVNKTAVDNNFFNAKRSVLCLPPPPIQNCFPYPIAGTSDSQQMDMSEDSNDGPSEKRSRDISSHDSPNSSTNDEVRRIVESGTEKLVLGSSIWAAPGQFSRTQQNQGAPGTSRQVRPLPDFQSQDSARSPGAFRSTAKPVCRMTVNTGNHGDVGVVVVDERNREHVIHAQHIVNALSSLRTTPSMRESPVGRKRMHPHTSNSFEFLNCNQEMNKNEGALGSDISPTHTAVSNLISRESSGYMASPTKFTTARGDTTSFSKIFQKIEEKHLQHNQPSTSYCNSQIQPPILSSKPVDSSVKLIAPVAVKPIMSGCNSIIPSPITTPRITPSFRMLEDDSLINVLGQLAHSNDGTTLNDSFIQHLIDTNSRSPLLSSGNAFSALSMGAVSGLVPGNSIHRPDSSASNGSNSLGVAMGLAVPQNIALAVQQTQNAMSPLHQIRVSVGAPPACSPSSSNSSLGAANQAPVSNTPQDPNAPKLPTDFSHALRNEKK

Target ORF information:

RefSeq Version	NM_001027335.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans NFI (Nuclear Factor I) family (nfi-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027335.3

ORF Insert Sequence:

ATGCCTCCAC CATCTTCTGA TTGGAGTAAT CAATTCAATT CTCCAGAAGC CGTGAGTCCG 
AAAGCCAATG GTATCAAATG CTTCAGTCCT TACTCACAGG AAGATATGGG ACCATTTGTC 
GAACAACTTC TACCTTTTGT CAGAGCAAGT GCCTATAATT GGTTTCATCT GCAGGCAGCA 
AAACGACGGC ACTTCAAAGA ATTTGATAAG AAGATGTGTG CGAGTGAAGA AAACGCGAAA 
TTAGCAGAAT TACAAAATGA TAGAGATGAA TTAAAAGTGA AATGGGCTTC CAGACTTTTG 
GGAAAAATAA AAAAAGATAT TCAAAATGAC GATAAAGAAG CGTTTATTTC GGCTATCAAT 
GGAAGTGAGC CGAACAAGTG TATTATATCA GTTGCTGATC AGAAAGGGAA AATGCGGCGA 
ATTGATTGCT TACGCCAAGC TGACAAAGTT TGGAGGCTTG ATCTGGTTAC TATTATTTTA 
TTTAAAGGAA TTCCTCTCGA GAGCACCGAC GGCGAGCGTC TCGAACGATC CGAGGCATGT 
GTCCACCCTC TGTGCATAAA TCCTTTTCAT ATGGCGATTA GTGTTCGCGG TCTTGACGTT 
TTTATGGCAA ACTATCTGAA AGACGTTGAC ACTAAAATTA CATTGACCTA TCCGAGAAAT 
GATGAACTTT CTGATACAGT GATGGTAAAG CAAGAGCCCG GTGAACAGAC AATTGTAGCT 
CCTCATGCAG TTCTTGGCAC AAGTTCTACT CATACTCGTG TCTGGGAAAC GAATTCCGAA 
CGAAATGCGG AAACAATCAT TATCACTTAT GATCCACAGA AAGCTTGCCA TACTTATTTT 
GGTGGTCGTG CTACTCTTGC CCAACAGTCT CTTTCTGCGG GAAACACGTA TATGGTTAAC 
AAAACCGCAG TCGACAACAA CTTTTTCAAT GCAAAAAGAT CTGTTCTATG TCTACCACCT 
CCTCCGATTC AAAATTGTTT TCCTTATCCC ATCGCGGGAA CATCTGATTC TCAACAAATG 
GATATGTCGG AGGATTCCAA CGATGGACCT TCAGAGAAAC GAAGTCGGGA TATATCATCG 
CATGATTCTC CTAATTCCAG CACTAACGAC GAAGTTCGGC GGATAGTGGA AAGTGGAACG 
GAAAAATTGG TACTCGGAAG CTCCATATGG GCAGCACCAG GCCAATTCTC TCGTACACAA 
CAAAATCAAG GAGCTCCCGG AACGAGTCGG CAAGTGAGAC CGCTTCCCGA TTTTCAAAGT 
CAAGATTCTG CGAGGAGTCC GGGTGCATTC CGTTCAACAG CAAAACCCGT TTGTCGAATG 
ACAGTGAATA CTGGAAATCA TGGCGATGTT GGAGTCGTTG TTGTTGATGA AAGAAATCGG 
GAGCATGTGA TTCATGCTCA ACATATAGTG AATGCATTGA GCTCACTTCG AACGACACCA 
TCGATGCGAG AAAGTCCAGT TGGCAGAAAA AGAATGCATC CGCATACGAG CAATTCATTT 
GAATTTTTGA ATTGCAATCA GGAAATGAAT AAAAATGAAG GAGCTTTAGG AAGCGATATT 
TCACCGACTC ACACTGCTGT TTCTAATCTA ATCAGCAGAG AAAGTAGTGG ATACATGGCA 
AGCCCAACAA AATTCACAAC AGCACGCGGA GATACAACAA GCTTCAGCAA AATTTTCCAA 
AAGATCGAAG AGAAACATCT GCAACATAAT CAACCGAGCA CGAGCTACTG TAATTCACAA 
ATCCAACCAC CAATCTTATC ATCGAAACCA GTTGATTCAT CAGTGAAATT AATTGCTCCA 
GTTGCAGTGA AACCAATTAT GTCAGGATGT AATTCAATCA TTCCATCACC CATCACAACT 
CCTCGTATAA CTCCTTCATT TCGAATGCTT GAAGATGATT CATTGATTAA TGTACTTGGA 
CAACTTGCAC ACAGTAATGA CGGAACAACT TTAAACGATA GCTTCATTCA ACATCTTATT 
GATACCAATT CTCGATCTCC ATTGCTTAGT AGTGGAAATG CTTTTTCGGC ATTATCAATG 
GGAGCTGTGA GTGGATTAGT TCCTGGTAAT TCGATTCATC GTCCGGATAG TTCTGCAAGC 
AACGGATCGA ATTCGTTGGG TGTAGCAATG GGATTGGCCG TTCCTCAAAA TATTGCACTG 
GCCGTCCAGC AAACTCAAAA TGCAATGTCA CCTCTTCATC AAATTCGTGT TAGCGTTGGA 
GCTCCACCAG CATGCTCCCC GTCATCATCA AATTCATCTC TAGGAGCTGC AAATCAAGCA 
CCGGTCTCAA ATACTCCACA AGATCCGAAT GCTCCAAAGC TGCCAACTGA CTTTTCACAT 
GCTCTTCGTA ATGAGAAAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfi-1 ( NM_001027336.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022507.1 Caenorhabditis elegans NFI (Nuclear Factor I) family (nfi-1), partial mRNA.

Price & Availability↑

CloneID	OCa338796
Clone ID Related Accession (Same CDS sequence)	NM_001027336.3 , NM_001027336.4
Accession Version	NM_001027336.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	NFI (Nuclear Factor I) family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_001027336.2. COMPLETENESS: incomplete on both ends.

ATGGAGCCGC ATCTCAAGAT CGATGTCAGC TCCGCCAGCG GTTCGACGAC CACTGGTGCA 
ACAGCTTCAA CATCAGAAGC ACCTCAAGAC TCACAGGCAC AGCAGACAAT GCCTCCACCA 
TCTTCTGATT GGAGTAATCA ATTCAATTCT CCAGAAGCCG TGAGTCCGAA AGCCAATGGT 
ATCAAATGCT TCAGTCCTTA CTCACAGGAA GATATGGGAC CATTTGTCGA ACAACTTCTA 
CCTTTTGTCA GAGCAAGTGC CTATAATTGG TTTCATCTGC AGGCAGCAAA ACGACGGCAC 
TTCAAAGAAT TTGATAAGAA GATGTGTGCG AGTGAAGAAA ACGCGAAATT AGCAGAATTA 
CAAAATGATA GAGATGAATT AAAAGTGAAA TGGGCTTCCA GACTTTTGGG AAAAATAAAA 
AAAGATATTC AAAATGACGA TAAAGAAGCG TTTATTTCGG CTATCAATGG AAGTGAGCCG 
AACAAGTGTA TTATATCAGT TGCTGATCAG AAAGGGAAAA TGCGGCGAAT TGATTGCTTA 
CGCCAAGCTG ACAAAGTTTG GAGGCTTGAT CTGGTTACTA TTATTTTATT TAAAGGAATT 
CCTCTCGAGA GCACCGACGG CGAGCGTCTC GAACGATCCG AGGCATGTGT CCACCCTCTG 
TGCATAAATC CTTTTCATAT GGCGATTAGT GTTCGCGGTC TTGACGTTTT TATGGCAAAC 
TATCTGAAAG ACGTTGACAC TAAAATTACA TTGACCTATC CGAGAAATGA TGAACTTTCT 
GATACAGTGA TGGTAAAGCA AGAGCCCGGT GAACAGACAA TTGTAGCTCC TCATGCAGTT 
CTTGGCACAA GTTCTACTCA TACTCGTGTC TGGGAAACGA ATTCCGAACG AAATGCGGAA 
ACAATCATTA TCACTTATGA TCCACAGAAA GCTTGCCATA CTTATTTTGG TGGTCGTGCT 
ACTCTTGCCC AACAGTCTCT TTCTGCGGGA AACACGTATA TGGTTAACAA AACCGCAGTC 
GACAACAACT TTTTCAATGC AAAAAGATCT GTTCTATGTC TACCACCTCC TCCGATTCAA 
AATTGTTTTC CTTATCCCAT CGCGGGAACA TCTGATTCTC AACAAATGGA TATGTCGGAG 
GATTCCAACG ATGGACCTTC AGAGAAACGA AGTCGGGATA TATCATCGCA TGATTCTCCT 
AATTCCAGCA CTAACGACGA AGTTCGGCGG ATAGTGGAAA GTGGAACGGA AAAATTGGTA 
CTCGGAAGCT CCATATGGGC AGCACCAGGC CAATTCTCTC GTACACAACA AAATCAAGGA 
GCTCCCGGAA CGAGTCGGCA AGTGAGACCG CTTCCCGATT TTCAAAGTCA AGATTCTGCG 
AGGAGTCCGG GTGCATTCCG TTCAACAGCA AAACCCGTTT GTCGAATGAC AGTGAATACT 
GGAAATCATG GCGATGTTGG AGTCGTTGTT GTTGATGAAA GAAATCGGGA GCATGTGATT 
CATGCTCAAC ATATAGTGAA TGCATTGAGC TCACTTCGAA CGACACCATC GATGCGAGAA 
AGTCCAGTTG GCAGAAAAAG AATGCATCCG CATACGAGCA ATTCATTTGA ATTTTTGAAT 
TGCAATCAGG AAATGAATAA AAATGAAGGA GCTTTAGGAA GCGATATTTC ACCGACTCAC 
ACTGCTGTTT CTAATCTAAT CAGCAGAGAA AGTAGTGGAT ACATGGCAAG CCCAACAAAA 
TTCACAACAG CACGCGGAGA TACAACAAGC TTCAGCAAAA TTTTCCAAAA GATCGAAGAG 
AAACATCTGC AACATAATCA ACCGAGCACG AGCTACTGTA ATTCACAAAT CCAACCACCA 
ATCTTATCAT CGAAACCAGT TGATTCATCA GTGAAATTAA TTGCTCCAGT TGCAGTGAAA 
CCAATTATGT CAGGATGTAA TTCAATCATT CCATCACCCA TCACAACTCC TCGTATAACT 
CCTTCATTTC GAATGCTTGA AGATGATTCA TTGATTAATG TACTTGGACA ACTTGCACAC 
AGTAATGACG GAACAACTTT AAACGATAGC TTCATTCAAC ATCTTATTGA TACCAATTCT 
CGATCTCCAT TGCTTAGTAG TGGAAATGCT TTTTCGGCAT TATCAATGGG AGCTGTGAGT 
GGATTAGTTC CTGGTAATTC GATTCATCGT CCGGATAGTT CTGCAAGCAA CGGATCGAAT 
TCGTTGGGTG TAGCAATGGG ATTGGCCGTT CCTCAAAATA TTGCACTGGC CGTCCAGCAA 
ACTCAAAATG CAATGTCACC TCTTCATCAA ATTCGTGTTA GCGTTGGAGC TCCACCAGCA 
TGCTCCCCGT CATCATCAAA TTCATCTCTA GGAGCTGCAA ATCAAGCACC GGTCTCAAAT 
ACTCCACAAG ATCCGAATGC TCCAAAGCTG CCAACTGACT TTTCACATGC TCTTCGTAAT 
GAGAAAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022507.1
CDS	1..2472
Translation	MEPHLKIDVSSASGSTTTGATASTSEAPQDSQAQQTMPPPSSDWSNQFNSPEAVSPKANGIKCFSPYSQEDMGPFVEQLLPFVRASAYNWFHLQAAKRRHFKEFDKKMCASEENAKLAELQNDRDELKVKWASRLLGKIKKDIQNDDKEAFISAINGSEPNKCIISVADQKGKMRRIDCLRQADKVWRLDLVTIILFKGIPLESTDGERLERSEACVHPLCINPFHMAISVRGLDVFMANYLKDVDTKITLTYPRNDELSDTVMVKQEPGEQTIVAPHAVLGTSSTHTRVWETNSERNAETIIITYDPQKACHTYFGGRATLAQQSLSAGNTYMVNKTAVDNNFFNAKRSVLCLPPPPIQNCFPYPIAGTSDSQQMDMSEDSNDGPSEKRSRDISSHDSPNSSTNDEVRRIVESGTEKLVLGSSIWAAPGQFSRTQQNQGAPGTSRQVRPLPDFQSQDSARSPGAFRSTAKPVCRMTVNTGNHGDVGVVVVDERNREHVIHAQHIVNALSSLRTTPSMRESPVGRKRMHPHTSNSFEFLNCNQEMNKNEGALGSDISPTHTAVSNLISRESSGYMASPTKFTTARGDTTSFSKIFQKIEEKHLQHNQPSTSYCNSQIQPPILSSKPVDSSVKLIAPVAVKPIMSGCNSIIPSPITTPRITPSFRMLEDDSLINVLGQLAHSNDGTTLNDSFIQHLIDTNSRSPLLSSGNAFSALSMGAVSGLVPGNSIHRPDSSASNGSNSLGVAMGLAVPQNIALAVQQTQNAMSPLHQIRVSVGAPPACSPSSSNSSLGAANQAPVSNTPQDPNAPKLPTDFSHALRNEKK

Target ORF information:

RefSeq Version	NM_001027336.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans NFI (Nuclear Factor I) family (nfi-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027336.3

ORF Insert Sequence:

ATGGAGCCGC ATCTCAAGAT CGATGTCAGC TCCGCCAGCG GTTCGACGAC CACTGGTGCA 
ACAGCTTCAA CATCAGAAGC ACCTCAAGAC TCACAGGCAC AGCAGACAAT GCCTCCACCA 
TCTTCTGATT GGAGTAATCA ATTCAATTCT CCAGAAGCCG TGAGTCCGAA AGCCAATGGT 
ATCAAATGCT TCAGTCCTTA CTCACAGGAA GATATGGGAC CATTTGTCGA ACAACTTCTA 
CCTTTTGTCA GAGCAAGTGC CTATAATTGG TTTCATCTGC AGGCAGCAAA ACGACGGCAC 
TTCAAAGAAT TTGATAAGAA GATGTGTGCG AGTGAAGAAA ACGCGAAATT AGCAGAATTA 
CAAAATGATA GAGATGAATT AAAAGTGAAA TGGGCTTCCA GACTTTTGGG AAAAATAAAA 
AAAGATATTC AAAATGACGA TAAAGAAGCG TTTATTTCGG CTATCAATGG AAGTGAGCCG 
AACAAGTGTA TTATATCAGT TGCTGATCAG AAAGGGAAAA TGCGGCGAAT TGATTGCTTA 
CGCCAAGCTG ACAAAGTTTG GAGGCTTGAT CTGGTTACTA TTATTTTATT TAAAGGAATT 
CCTCTCGAGA GCACCGACGG CGAGCGTCTC GAACGATCCG AGGCATGTGT CCACCCTCTG 
TGCATAAATC CTTTTCATAT GGCGATTAGT GTTCGCGGTC TTGACGTTTT TATGGCAAAC 
TATCTGAAAG ACGTTGACAC TAAAATTACA TTGACCTATC CGAGAAATGA TGAACTTTCT 
GATACAGTGA TGGTAAAGCA AGAGCCCGGT GAACAGACAA TTGTAGCTCC TCATGCAGTT 
CTTGGCACAA GTTCTACTCA TACTCGTGTC TGGGAAACGA ATTCCGAACG AAATGCGGAA 
ACAATCATTA TCACTTATGA TCCACAGAAA GCTTGCCATA CTTATTTTGG TGGTCGTGCT 
ACTCTTGCCC AACAGTCTCT TTCTGCGGGA AACACGTATA TGGTTAACAA AACCGCAGTC 
GACAACAACT TTTTCAATGC AAAAAGATCT GTTCTATGTC TACCACCTCC TCCGATTCAA 
AATTGTTTTC CTTATCCCAT CGCGGGAACA TCTGATTCTC AACAAATGGA TATGTCGGAG 
GATTCCAACG ATGGACCTTC AGAGAAACGA AGTCGGGATA TATCATCGCA TGATTCTCCT 
AATTCCAGCA CTAACGACGA AGTTCGGCGG ATAGTGGAAA GTGGAACGGA AAAATTGGTA 
CTCGGAAGCT CCATATGGGC AGCACCAGGC CAATTCTCTC GTACACAACA AAATCAAGGA 
GCTCCCGGAA CGAGTCGGCA AGTGAGACCG CTTCCCGATT TTCAAAGTCA AGATTCTGCG 
AGGAGTCCGG GTGCATTCCG TTCAACAGCA AAACCCGTTT GTCGAATGAC AGTGAATACT 
GGAAATCATG GCGATGTTGG AGTCGTTGTT GTTGATGAAA GAAATCGGGA GCATGTGATT 
CATGCTCAAC ATATAGTGAA TGCATTGAGC TCACTTCGAA CGACACCATC GATGCGAGAA 
AGTCCAGTTG GCAGAAAAAG AATGCATCCG CATACGAGCA ATTCATTTGA ATTTTTGAAT 
TGCAATCAGG AAATGAATAA AAATGAAGGA GCTTTAGGAA GCGATATTTC ACCGACTCAC 
ACTGCTGTTT CTAATCTAAT CAGCAGAGAA AGTAGTGGAT ACATGGCAAG CCCAACAAAA 
TTCACAACAG CACGCGGAGA TACAACAAGC TTCAGCAAAA TTTTCCAAAA GATCGAAGAG 
AAACATCTGC AACATAATCA ACCGAGCACG AGCTACTGTA ATTCACAAAT CCAACCACCA 
ATCTTATCAT CGAAACCAGT TGATTCATCA GTGAAATTAA TTGCTCCAGT TGCAGTGAAA 
CCAATTATGT CAGGATGTAA TTCAATCATT CCATCACCCA TCACAACTCC TCGTATAACT 
CCTTCATTTC GAATGCTTGA AGATGATTCA TTGATTAATG TACTTGGACA ACTTGCACAC 
AGTAATGACG GAACAACTTT AAACGATAGC TTCATTCAAC ATCTTATTGA TACCAATTCT 
CGATCTCCAT TGCTTAGTAG TGGAAATGCT TTTTCGGCAT TATCAATGGG AGCTGTGAGT 
GGATTAGTTC CTGGTAATTC GATTCATCGT CCGGATAGTT CTGCAAGCAA CGGATCGAAT 
TCGTTGGGTG TAGCAATGGG ATTGGCCGTT CCTCAAAATA TTGCACTGGC CGTCCAGCAA 
ACTCAAAATG CAATGTCACC TCTTCATCAA ATTCGTGTTA GCGTTGGAGC TCCACCAGCA 
TGCTCCCCGT CATCATCAAA TTCATCTCTA GGAGCTGCAA ATCAAGCACC GGTCTCAAAT 
ACTCCACAAG ATCCGAATGC TCCAAAGCTG CCAACTGACT TTTCACATGC TCTTCGTAAT 
GAGAAAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfi-1 ( NM_001027336.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022507.1 Caenorhabditis elegans CTF/NF-I domain-containing protein (nfi-1), mRNA.

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CloneID	OCa338796
Clone ID Related Accession (Same CDS sequence)	NM_001027336.3 , NM_001027336.4
Accession Version	NM_001027336.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	CTF/NF-I domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001027336.3.

ATGGAGCCGC ATCTCAAGAT CGATGTCAGC TCCGCCAGCG GTTCGACGAC CACTGGTGCA 
ACAGCTTCAA CATCAGAAGC ACCTCAAGAC TCACAGGCAC AGCAGACAAT GCCTCCACCA 
TCTTCTGATT GGAGTAATCA ATTCAATTCT CCAGAAGCCG TGAGTCCGAA AGCCAATGGT 
ATCAAATGCT TCAGTCCTTA CTCACAGGAA GATATGGGAC CATTTGTCGA ACAACTTCTA 
CCTTTTGTCA GAGCAAGTGC CTATAATTGG TTTCATCTGC AGGCAGCAAA ACGACGGCAC 
TTCAAAGAAT TTGATAAGAA GATGTGTGCG AGTGAAGAAA ACGCGAAATT AGCAGAATTA 
CAAAATGATA GAGATGAATT AAAAGTGAAA TGGGCTTCCA GACTTTTGGG AAAAATAAAA 
AAAGATATTC AAAATGACGA TAAAGAAGCG TTTATTTCGG CTATCAATGG AAGTGAGCCG 
AACAAGTGTA TTATATCAGT TGCTGATCAG AAAGGGAAAA TGCGGCGAAT TGATTGCTTA 
CGCCAAGCTG ACAAAGTTTG GAGGCTTGAT CTGGTTACTA TTATTTTATT TAAAGGAATT 
CCTCTCGAGA GCACCGACGG CGAGCGTCTC GAACGATCCG AGGCATGTGT CCACCCTCTG 
TGCATAAATC CTTTTCATAT GGCGATTAGT GTTCGCGGTC TTGACGTTTT TATGGCAAAC 
TATCTGAAAG ACGTTGACAC TAAAATTACA TTGACCTATC CGAGAAATGA TGAACTTTCT 
GATACAGTGA TGGTAAAGCA AGAGCCCGGT GAACAGACAA TTGTAGCTCC TCATGCAGTT 
CTTGGCACAA GTTCTACTCA TACTCGTGTC TGGGAAACGA ATTCCGAACG AAATGCGGAA 
ACAATCATTA TCACTTATGA TCCACAGAAA GCTTGCCATA CTTATTTTGG TGGTCGTGCT 
ACTCTTGCCC AACAGTCTCT TTCTGCGGGA AACACGTATA TGGTTAACAA AACCGCAGTC 
GACAACAACT TTTTCAATGC AAAAAGATCT GTTCTATGTC TACCACCTCC TCCGATTCAA 
AATTGTTTTC CTTATCCCAT CGCGGGAACA TCTGATTCTC AACAAATGGA TATGTCGGAG 
GATTCCAACG ATGGACCTTC AGAGAAACGA AGTCGGGATA TATCATCGCA TGATTCTCCT 
AATTCCAGCA CTAACGACGA AGTTCGGCGG ATAGTGGAAA GTGGAACGGA AAAATTGGTA 
CTCGGAAGCT CCATATGGGC AGCACCAGGC CAATTCTCTC GTACACAACA AAATCAAGGA 
GCTCCCGGAA CGAGTCGGCA AGTGAGACCG CTTCCCGATT TTCAAAGTCA AGATTCTGCG 
AGGAGTCCGG GTGCATTCCG TTCAACAGCA AAACCCGTTT GTCGAATGAC AGTGAATACT 
GGAAATCATG GCGATGTTGG AGTCGTTGTT GTTGATGAAA GAAATCGGGA GCATGTGATT 
CATGCTCAAC ATATAGTGAA TGCATTGAGC TCACTTCGAA CGACACCATC GATGCGAGAA 
AGTCCAGTTG GCAGAAAAAG AATGCATCCG CATACGAGCA ATTCATTTGA ATTTTTGAAT 
TGCAATCAGG AAATGAATAA AAATGAAGGA GCTTTAGGAA GCGATATTTC ACCGACTCAC 
ACTGCTGTTT CTAATCTAAT CAGCAGAGAA AGTAGTGGAT ACATGGCAAG CCCAACAAAA 
TTCACAACAG CACGCGGAGA TACAACAAGC TTCAGCAAAA TTTTCCAAAA GATCGAAGAG 
AAACATCTGC AACATAATCA ACCGAGCACG AGCTACTGTA ATTCACAAAT CCAACCACCA 
ATCTTATCAT CGAAACCAGT TGATTCATCA GTGAAATTAA TTGCTCCAGT TGCAGTGAAA 
CCAATTATGT CAGGATGTAA TTCAATCATT CCATCACCCA TCACAACTCC TCGTATAACT 
CCTTCATTTC GAATGCTTGA AGATGATTCA TTGATTAATG TACTTGGACA ACTTGCACAC 
AGTAATGACG GAACAACTTT AAACGATAGC TTCATTCAAC ATCTTATTGA TACCAATTCT 
CGATCTCCAT TGCTTAGTAG TGGAAATGCT TTTTCGGCAT TATCAATGGG AGCTGTGAGT 
GGATTAGTTC CTGGTAATTC GATTCATCGT CCGGATAGTT CTGCAAGCAA CGGATCGAAT 
TCGTTGGGTG TAGCAATGGG ATTGGCCGTT CCTCAAAATA TTGCACTGGC CGTCCAGCAA 
ACTCAAAATG CAATGTCACC TCTTCATCAA ATTCGTGTTA GCGTTGGAGC TCCACCAGCA 
TGCTCCCCGT CATCATCAAA TTCATCTCTA GGAGCTGCAA ATCAAGCACC GGTCTCAAAT 
ACTCCACAAG ATCCGAATGC TCCAAAGCTG CCAACTGACT TTTCACATGC TCTTCGTAAT 
GAGAAAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022507.1
CDS	87..2558
Translation	MEPHLKIDVSSASGSTTTGATASTSEAPQDSQAQQTMPPPSSDWSNQFNSPEAVSPKANGIKCFSPYSQEDMGPFVEQLLPFVRASAYNWFHLQAAKRRHFKEFDKKMCASEENAKLAELQNDRDELKVKWASRLLGKIKKDIQNDDKEAFISAINGSEPNKCIISVADQKGKMRRIDCLRQADKVWRLDLVTIILFKGIPLESTDGERLERSEACVHPLCINPFHMAISVRGLDVFMANYLKDVDTKITLTYPRNDELSDTVMVKQEPGEQTIVAPHAVLGTSSTHTRVWETNSERNAETIIITYDPQKACHTYFGGRATLAQQSLSAGNTYMVNKTAVDNNFFNAKRSVLCLPPPPIQNCFPYPIAGTSDSQQMDMSEDSNDGPSEKRSRDISSHDSPNSSTNDEVRRIVESGTEKLVLGSSIWAAPGQFSRTQQNQGAPGTSRQVRPLPDFQSQDSARSPGAFRSTAKPVCRMTVNTGNHGDVGVVVVDERNREHVIHAQHIVNALSSLRTTPSMRESPVGRKRMHPHTSNSFEFLNCNQEMNKNEGALGSDISPTHTAVSNLISRESSGYMASPTKFTTARGDTTSFSKIFQKIEEKHLQHNQPSTSYCNSQIQPPILSSKPVDSSVKLIAPVAVKPIMSGCNSIIPSPITTPRITPSFRMLEDDSLINVLGQLAHSNDGTTLNDSFIQHLIDTNSRSPLLSSGNAFSALSMGAVSGLVPGNSIHRPDSSASNGSNSLGVAMGLAVPQNIALAVQQTQNAMSPLHQIRVSVGAPPACSPSSSNSSLGAANQAPVSNTPQDPNAPKLPTDFSHALRNEKK

Target ORF information:

RefSeq Version	NM_001027336.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans CTF/NF-I domain-containing protein (nfi-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027336.4

ORF Insert Sequence:

ATGGAGCCGC ATCTCAAGAT CGATGTCAGC TCCGCCAGCG GTTCGACGAC CACTGGTGCA 
ACAGCTTCAA CATCAGAAGC ACCTCAAGAC TCACAGGCAC AGCAGACAAT GCCTCCACCA 
TCTTCTGATT GGAGTAATCA ATTCAATTCT CCAGAAGCCG TGAGTCCGAA AGCCAATGGT 
ATCAAATGCT TCAGTCCTTA CTCACAGGAA GATATGGGAC CATTTGTCGA ACAACTTCTA 
CCTTTTGTCA GAGCAAGTGC CTATAATTGG TTTCATCTGC AGGCAGCAAA ACGACGGCAC 
TTCAAAGAAT TTGATAAGAA GATGTGTGCG AGTGAAGAAA ACGCGAAATT AGCAGAATTA 
CAAAATGATA GAGATGAATT AAAAGTGAAA TGGGCTTCCA GACTTTTGGG AAAAATAAAA 
AAAGATATTC AAAATGACGA TAAAGAAGCG TTTATTTCGG CTATCAATGG AAGTGAGCCG 
AACAAGTGTA TTATATCAGT TGCTGATCAG AAAGGGAAAA TGCGGCGAAT TGATTGCTTA 
CGCCAAGCTG ACAAAGTTTG GAGGCTTGAT CTGGTTACTA TTATTTTATT TAAAGGAATT 
CCTCTCGAGA GCACCGACGG CGAGCGTCTC GAACGATCCG AGGCATGTGT CCACCCTCTG 
TGCATAAATC CTTTTCATAT GGCGATTAGT GTTCGCGGTC TTGACGTTTT TATGGCAAAC 
TATCTGAAAG ACGTTGACAC TAAAATTACA TTGACCTATC CGAGAAATGA TGAACTTTCT 
GATACAGTGA TGGTAAAGCA AGAGCCCGGT GAACAGACAA TTGTAGCTCC TCATGCAGTT 
CTTGGCACAA GTTCTACTCA TACTCGTGTC TGGGAAACGA ATTCCGAACG AAATGCGGAA 
ACAATCATTA TCACTTATGA TCCACAGAAA GCTTGCCATA CTTATTTTGG TGGTCGTGCT 
ACTCTTGCCC AACAGTCTCT TTCTGCGGGA AACACGTATA TGGTTAACAA AACCGCAGTC 
GACAACAACT TTTTCAATGC AAAAAGATCT GTTCTATGTC TACCACCTCC TCCGATTCAA 
AATTGTTTTC CTTATCCCAT CGCGGGAACA TCTGATTCTC AACAAATGGA TATGTCGGAG 
GATTCCAACG ATGGACCTTC AGAGAAACGA AGTCGGGATA TATCATCGCA TGATTCTCCT 
AATTCCAGCA CTAACGACGA AGTTCGGCGG ATAGTGGAAA GTGGAACGGA AAAATTGGTA 
CTCGGAAGCT CCATATGGGC AGCACCAGGC CAATTCTCTC GTACACAACA AAATCAAGGA 
GCTCCCGGAA CGAGTCGGCA AGTGAGACCG CTTCCCGATT TTCAAAGTCA AGATTCTGCG 
AGGAGTCCGG GTGCATTCCG TTCAACAGCA AAACCCGTTT GTCGAATGAC AGTGAATACT 
GGAAATCATG GCGATGTTGG AGTCGTTGTT GTTGATGAAA GAAATCGGGA GCATGTGATT 
CATGCTCAAC ATATAGTGAA TGCATTGAGC TCACTTCGAA CGACACCATC GATGCGAGAA 
AGTCCAGTTG GCAGAAAAAG AATGCATCCG CATACGAGCA ATTCATTTGA ATTTTTGAAT 
TGCAATCAGG AAATGAATAA AAATGAAGGA GCTTTAGGAA GCGATATTTC ACCGACTCAC 
ACTGCTGTTT CTAATCTAAT CAGCAGAGAA AGTAGTGGAT ACATGGCAAG CCCAACAAAA 
TTCACAACAG CACGCGGAGA TACAACAAGC TTCAGCAAAA TTTTCCAAAA GATCGAAGAG 
AAACATCTGC AACATAATCA ACCGAGCACG AGCTACTGTA ATTCACAAAT CCAACCACCA 
ATCTTATCAT CGAAACCAGT TGATTCATCA GTGAAATTAA TTGCTCCAGT TGCAGTGAAA 
CCAATTATGT CAGGATGTAA TTCAATCATT CCATCACCCA TCACAACTCC TCGTATAACT 
CCTTCATTTC GAATGCTTGA AGATGATTCA TTGATTAATG TACTTGGACA ACTTGCACAC 
AGTAATGACG GAACAACTTT AAACGATAGC TTCATTCAAC ATCTTATTGA TACCAATTCT 
CGATCTCCAT TGCTTAGTAG TGGAAATGCT TTTTCGGCAT TATCAATGGG AGCTGTGAGT 
GGATTAGTTC CTGGTAATTC GATTCATCGT CCGGATAGTT CTGCAAGCAA CGGATCGAAT 
TCGTTGGGTG TAGCAATGGG ATTGGCCGTT CCTCAAAATA TTGCACTGGC CGTCCAGCAA 
ACTCAAAATG CAATGTCACC TCTTCATCAA ATTCGTGTTA GCGTTGGAGC TCCACCAGCA 
TGCTCCCCGT CATCATCAAA TTCATCTCTA GGAGCTGCAA ATCAAGCACC GGTCTCAAAT 
ACTCCACAAG ATCCGAATGC TCCAAAGCTG CCAACTGACT TTTCACATGC TCTTCGTAAT 
GAGAAAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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nfi-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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