GEA2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	GEA2
Entrez Gene ID	856691
Full Name	Arf family guanine nucleotide exchange factor GEA2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178837.1	NP_010892.1	Arf family guanine nucleotide exchange factor GEA2

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E22727g	XP_454979.1
Saccharomyces cerevisiae (baker's yeast)	GEA2	NP_010892.1
Neurospora crassa	NCU08023	XP_962693.2
Eremothecium gossypii	AGOS_AEL061W	NP_984800.2
Schizosaccharomyces pombe (fission yeast)	SPBC211.03c	NP_596613.1
Magnaporthe oryzae (rice blast fungus)	MGG_12336	XP_003715273.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results demonstrate that activated Arl1p can promote the spatial modulation of membrane organization at the trans-Golgi network through interacting with the effectors Gea2p and Drs2p
Title: Arl1p regulates spatial membrane organization at the trans-Golgi network through interaction with Arf-GEF Gea2p and flippase Drs2p.

basic residues required for phosphoinositide binding overlap a previously mapped binding site for the ArfGEF Gea2p.
Title: Regulation of a Golgi flippase by phosphoinositides and an ArfGEF.

COPI in these mutants is released from Golgi membranes by brefeldin A, a drug that binds directly to Gea2p and blocks ADP-ribosylation factor activation
Title: Mutations in a highly conserved region of the Arf1p activator GEA2 block anterograde Golgi transport but not COPI recruitment to membranes.

The following GEA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GEA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05466	NM_001178837.1 Latest version!	Saccharomyces cerevisiae S288C Arf family guanine nucleotide exchange factor GEA2 (GEA2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GEA2 ( NM_001178837.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010892.1 Saccharomyces cerevisiae S288C Arf family guanine nucleotide exchange factor GEA2 (GEA2), partial mRNA.

Price & Availability↑

CloneID	OSi05466
Clone ID Related Accession (Same CDS sequence)	NM_001178837.1
Accession Version	NM_001178837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Arf family guanine nucleotide exchange factor GEA2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGTGATA GGGAATTCGT CACGGTAGAT CCCGTCACTA TCATAATCAA AGAATGCATT 
AATTTATCGA CAGCGATGCG GAAATACTCT AAATTTACCT CTCAATCTGG AGTGGCCGCT 
TTGCTGGGGG GAGGAAGTGA AATATTTAGC AATCAAGATG ACTACTTGGC TCACACATTC 
AACAATTTGA ATACCAACAA GCACAATGAT CCATTTTTAT CTGGATTCAT TCAGTTAAGA 
CTTATGTTGA ATAAACTGAA AAATCTAGAT AATATAGATT CACTAACCAT ATTGCAGCCA 
TTTTTATTAA TTGTGAGTAC AAGTTCCATT TCTGGTTACA TCACTTCCCT GGCCCTGGAC 
TCTTTGCAGA AATTCTTTAC CTTGAATATC ATCAATGAAT CATCGCAAAA CTATATTGGT 
GCACACAGGG CGACGGTAAA TGCTCTAACA CATTGTAGGT TTGAAGGATC TCAACAACTT 
TCTGATGATT CAGTTCTTTT GAAAGTCGTG TTTTTACTGC GTTCAATCGT CGACTCACCT 
TACGGAGATT TATTATCAAA CTCTATCATA TATGACGTAT TGCAAACGAT TCTTTCATTG 
GCTTGTAATA ACAGAAGGAG CGAAGTCCTT AGGAATGCTG CACAATCAAC AATGATAGCC 
GTTACCGTAA AGATTTTCTC AAAACTAAAG ACTATTGAGC CTGTTAATGT GAATCAAATA 
TACATCAATG ATGAAAGTTA CACAAATGAT GTATTGAAGG CCGATACAAT TGGCACAAAT 
GTAGAATCCA AAGAAGAAGG AAGTCAAGAA GATCCCATCG GCATGAAAGT GAATAATGAG 
GAAGCTATTA GCGAGGACGA TGGCATTGAA GAAGAGCATA TTCATTCAGA GAAGAGCACA 
AATGGCGCCG AACAACTAGA TATTGTGCAA AAAACAACAA GATCAAATTC CAGGATCCAA 
GCGTATGCTG ATGATAACTA TGGATTGCCC GTGGTTAGGC AATATTTAAA CTTATTACTA 
TCATTGATTG CGCCAGAAAA TGAATTAAAA CATTCATACT CCACTAGAAT ATTTGGCCTA 
GAGTTAATTC AAACGGCATT AGAAATTTCA GGTGATCGAT TGCAGCTATA CCCACGGCTT 
TTTACACTGA TATCAGATCC TATTTTCAAA AGCATTTTGT TTATCATACA GAACACTACA 
AAATTATCAC TACTTCAAGC TACATTGCAG CTATTTACTA CTCTAGTTGT TATATTGGGC 
AACAACTTAC AATTACAGAT CGAGCTCACT CTAACAAGAA TATTTTCTAT TCTTTTAGAT 
GATGGTACCG CAAATAACTC GAGTTCTGAA AATAAGAACA AGCCATCAAT AATAAAGGAA 
CTTCTAATTG AGCAAATATC CATCTTATGG ACTAGGTCGC CATCTTTTTT TACTTCTACT 
TTTATCAATT TCGATTGTAA TCTCGATAGG GCAGACGTTT CCATAAACTT TTTGAAGGCT 
TTGACTAAAT TGGCCTTACC AGAATCCGCC TTAACTACCA CAGAAAGTGT ACCACCCATT 
TGCCTTGAGG GATTGGTCTC CCTAGTCGAT GATATGTTCG ATCACATGAA GGACATTGAC 
AGAGAAGAAT TTGGCAGGCA AAAGAATGAA ATGGAAATCT TAAAAAAGAG GGACCGTAAA 
ACAGAGTTTA TTGAATGTAC CAATGCATTC AATGAAAAGC CCAAAAAGGG TATTCCGATG 
TTAATAGAAA AAGGTTTCAT TGCTTCCGAC TCCGATAAAG ATATTGCGGA GTTTCTTTTC 
AATAATAACA ACCGTATGAA TAAAAAAACA ATCGGTTTGC TACTTTGCCA TCCGGACAAA 
GTAAGCTTGT TGAATGAATA TATTCGTTTG TTTGATTTTT CAGGGTTAAG GGTCGATGAA 
GCTATTAGAA TTTTGTTGAC GAAATTTAGG TTGCCTGGTG AATCGCAACA AATTGAAAGA 
ATCATCGAAG CCTTCTCGTC TGCGTATTGT GAAAATCAAG ATTACGATCC ATCCAAAATC 
AGTGACAACG CGGAGGATGA CATTTCTACT GTTCAACCAG ACGCTGATTC TGTTTTCATT 
TTAAGTTATT CAATTATTAT GTTGAACACT GACCTACATA ACCCTCAAGT GAAGGAACAC 
ATGTCATTTG AAGATTACTC TGGTAACTTA AAGGGATGCT GTAATCACAA AGACTTCCCA 
TTCTGGTATT TGGATAGAAT TTACTGTTCA ATCAGAGATA AAGAAATTGT TATGCCTGAA 
GAGCACCACG GCAACGAAAA GTGGTTTGAA GATGCTTGGA ATAACTTGAT ATCTTCAACT 
ACTGTTATAA CTGAAATAAA AAAAGACACA CAATCTGTCA TGGATAAATT AACACCCTTG 
GAGCTTTTGA ACTTTGATAG AGCAATTTTT AAACAAGTTG GCCCAAGTAT TGTCAGTACT 
TTATTCAACA TTTACGTAGT TGCATCTGAT GACCATATAT CTACCAGAAT GATAACAAGT 
TTGGACAAAT GTTCCTATAT TTCCGCATTT TTTGACTTCA AAGATCTCTT TAATGATATA 
CTAAACTCCA TTGCTAAGGG CACTACTTTG ATTAATTCAA GCCATGACGA TGAACTTTCA 
ACTTTAGCTT TTGAATATGG CCCAATGCCA CTGGTGCAAA TTAAATTCGA AGACACTAAC 
ACTGAGATCC CGGTTAGTAC AGATGCTGTT AGATTTGGTA GATCATTTAA GGGTCAACTA 
AATACAGTTG TTTTTTTCCG GATTATTCGC AGGAACAAAG ATCCTAAAAT TTTCTCCAAG 
GAATTATGGT TAAACATTGT TAATATTATA CTAACATTGT ACGAAGACTT GATTTTGTCT 
CCTGATATTT TCCCTGATTT ACAAAAAAGA CTGAAATTAA GCAACTTGCC TAAGCCATCT 
CCTGAAATTT CTATTAACAA GAGCAAAGAA AGCAAAGGTC TCTTATCAAC ATTTGCTTCT 
TATTTAAAAG GTGATGAAGA ACCCACAGAA GAGGAAATCA AATCCTCAAA AAAAGCGATG 
GAGTGCATAA AGTCGAGTAA TATTGCCGCC TCTGTCTTTG GAAATGAATC AAATATAACA 
GCGGATTTAA TAAAAACTTT ACTAGACTCC GCCAAAACTG AGAAAAACGC AGATAATTCC 
AGGTATTTTG AAGCAGAACT TTTATTTATC ATCGAATTGA CTATTGCATT ATTTCTATTT 
TGCAAAGAGG AGAAAGAATT AGGAAAGTTC ATACTTCAAA AAGTTTTCCA ACTTTCTCAC 
ACGAAAGGCC TCACGAAAAG GACTGTTCGT AGAATGCTAA CATACAAAAT TTTGTTAATT 
TCGTTATGTG CGGATCAGAC GGAGTACTTG TCCAAATTAA TAAACGATGA GCTGTTAAAA 
AAGGGGGATA TTTTTACCCA AAAATTTTTT GCAACTAATC AAGGTAAGGA ATTTTTGAAG 
AGACTATTTT CATTGACCGA ATCAGAGTTT TATAGAGGAT TTTTACTAGG AAATGAGAAT 
TTTTGGAAAT TTTTAAGAAA AGTTACAGCA ATGAAAGAGC AGAGCGAGAG CATTTTTGAA 
TATTTAAATG AATCGATCAA GACAGACAGC AATATTTTGA CAAATGAGAA CTTCATGTGG 
GTCCTAGGAC TATTAGATGA AATTTCATCA ATGGGTGCCG TTGGAAATCA CTGGGAAATA 
GAATACAAGA AATTGACAGA AAGTGGTCAT AAAATTGATA AGGAGAATCC ATACAAGAAA 
TCGATCGAAT TATCATTGAA ATCCATTCAA CTAACATCAC ACTTGCTGGA AGATAATAAC 
GATCTGCGTA AAAACGAGAT ATTCGCTATT ATTCAAGCTT TGGCACATCA ATGCATCAAT 
CCGTGTAAGC AGATAAGTGA ATTTGCAGTG GTAACGCTAG AGCAGACGCT CATCAATAAA 
ATCGAAATTC CAACTAATGA GATGGAATCG GTAGAAGAAT TAATTGAGGG CGGATTACTA 
CCGTTGCTAA ATTCGAGTGA AACACAGGAA GACCAGAAAA TCCTCATTTC ATCCATATTA 
ACAATAATTT CAAATGTTTA TTTGCATTAT TTGAAACTAG GGAAGACAAG CAACGAAACG 
TTTTTGAAAA TTTTGAGTAT TTTCAATAAA TTTGTAGAGG ACTCAGATAT TGAAAAAAAG 
CTACAGCAAT TAATACTTGA TAAGAAGAGT ATTGAGAAGG GCAACGGTTC ATCATCTCAT 
GGATCTGCAC ATGAACAAAC ACCAGAGTCA AACGACGTTG AAATTGAGGC TACTGCGCCA 
ATTGATGACA ATACAGACGA TGATAACAAA CCGAAGTTAT CTGATGTAGA AAAGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010892.1
CDS	1..4380
Translation	MSDREFVTVDPVTIIIKECINLSTAMRKYSKFTSQSGVAALLGGGSEIFSNQDDYLAHTFNNLNTNKHNDPFLSGFIQLRLMLNKLKNLDNIDSLTILQPFLLIVSTSSISGYITSLALDSLQKFFTLNIINESSQNYIGAHRATVNALTHCRFEGSQQLSDDSVLLKVVFLLRSIVDSPYGDLLSNSIIYDVLQTILSLACNNRRSEVLRNAAQSTMIAVTVKIFSKLKTIEPVNVNQIYINDESYTNDVLKADTIGTNVESKEEGSQEDPIGMKVNNEEAISEDDGIEEEHIHSEKSTNGAEQLDIVQKTTRSNSRIQAYADDNYGLPVVRQYLNLLLSLIAPENELKHSYSTRIFGLELIQTALEISGDRLQLYPRLFTLISDPIFKSILFIIQNTTKLSLLQATLQLFTTLVVILGNNLQLQIELTLTRIFSILLDDGTANNSSSENKNKPSIIKELLIEQISILWTRSPSFFTSTFINFDCNLDRADVSINFLKALTKLALPESALTTTESVPPICLEGLVSLVDDMFDHMKDIDREEFGRQKNEMEILKKRDRKTEFIECTNAFNEKPKKGIPMLIEKGFIASDSDKDIAEFLFNNNNRMNKKTIGLLLCHPDKVSLLNEYIRLFDFSGLRVDEAIRILLTKFRLPGESQQIERIIEAFSSAYCENQDYDPSKISDNAEDDISTVQPDADSVFILSYSIIMLNTDLHNPQVKEHMSFEDYSGNLKGCCNHKDFPFWYLDRIYCSIRDKEIVMPEEHHGNEKWFEDAWNNLISSTTVITEIKKDTQSVMDKLTPLELLNFDRAIFKQVGPSIVSTLFNIYVVASDDHISTRMITSLDKCSYISAFFDFKDLFNDILNSIAKGTTLINSSHDDELSTLAFEYGPMPLVQIKFEDTNTEIPVSTDAVRFGRSFKGQLNTVVFFRIIRRNKDPKIFSKELWLNIVNIILTLYEDLILSPDIFPDLQKRLKLSNLPKPSPEISINKSKESKGLLSTFASYLKGDEEPTEEEIKSSKKAMECIKSSNIAASVFGNESNITADLIKTLLDSAKTEKNADNSRYFEAELLFIIELTIALFLFCKEEKELGKFILQKVFQLSHTKGLTKRTVRRMLTYKILLISLCADQTEYLSKLINDELLKKGDIFTQKFFATNQGKEFLKRLFSLTESEFYRGFLLGNENFWKFLRKVTAMKEQSESIFEYLNESIKTDSNILTNENFMWVLGLLDEISSMGAVGNHWEIEYKKLTESGHKIDKENPYKKSIELSLKSIQLTSHLLEDNNDLRKNEIFAIIQALAHQCINPCKQISEFAVVTLEQTLINKIEIPTNEMESVEELIEGGLLPLLNSSETQEDQKILISSILTIISNVYLHYLKLGKTSNETFLKILSIFNKFVEDSDIEKKLQQLILDKKSIEKGNGSSSHGSAHEQTPESNDVEIEATAPIDDNTDDDNKPKLSDVEKD

Target ORF information:

RefSeq Version	NM_001178837.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Arf family guanine nucleotide exchange factor GEA2 (GEA2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178837.1

ORF Insert Sequence:

ATGAGTGATA GGGAATTCGT CACGGTAGAT CCCGTCACTA TCATAATCAA AGAATGCATT 
AATTTATCGA CAGCGATGCG GAAATACTCT AAATTTACCT CTCAATCTGG AGTGGCCGCT 
TTGCTGGGGG GAGGAAGTGA AATATTTAGC AATCAAGATG ACTACTTGGC TCACACATTC 
AACAATTTGA ATACCAACAA GCACAATGAT CCATTTTTAT CTGGATTCAT TCAGTTAAGA 
CTTATGTTGA ATAAACTGAA AAATCTAGAT AATATAGATT CACTAACCAT ATTGCAGCCA 
TTTTTATTAA TTGTGAGTAC AAGTTCCATT TCTGGTTACA TCACTTCCCT GGCCCTGGAC 
TCTTTGCAGA AATTCTTTAC CTTGAATATC ATCAATGAAT CATCGCAAAA CTATATTGGT 
GCACACAGGG CGACGGTAAA TGCTCTAACA CATTGTAGGT TTGAAGGATC TCAACAACTT 
TCTGATGATT CAGTTCTTTT GAAAGTCGTG TTTTTACTGC GTTCAATCGT CGACTCACCT 
TACGGAGATT TATTATCAAA CTCTATCATA TATGACGTAT TGCAAACGAT TCTTTCATTG 
GCTTGTAATA ACAGAAGGAG CGAAGTCCTT AGGAATGCTG CACAATCAAC AATGATAGCC 
GTTACCGTAA AGATTTTCTC AAAACTAAAG ACTATTGAGC CTGTTAATGT GAATCAAATA 
TACATCAATG ATGAAAGTTA CACAAATGAT GTATTGAAGG CCGATACAAT TGGCACAAAT 
GTAGAATCCA AAGAAGAAGG AAGTCAAGAA GATCCCATCG GCATGAAAGT GAATAATGAG 
GAAGCTATTA GCGAGGACGA TGGCATTGAA GAAGAGCATA TTCATTCAGA GAAGAGCACA 
AATGGCGCCG AACAACTAGA TATTGTGCAA AAAACAACAA GATCAAATTC CAGGATCCAA 
GCGTATGCTG ATGATAACTA TGGATTGCCC GTGGTTAGGC AATATTTAAA CTTATTACTA 
TCATTGATTG CGCCAGAAAA TGAATTAAAA CATTCATACT CCACTAGAAT ATTTGGCCTA 
GAGTTAATTC AAACGGCATT AGAAATTTCA GGTGATCGAT TGCAGCTATA CCCACGGCTT 
TTTACACTGA TATCAGATCC TATTTTCAAA AGCATTTTGT TTATCATACA GAACACTACA 
AAATTATCAC TACTTCAAGC TACATTGCAG CTATTTACTA CTCTAGTTGT TATATTGGGC 
AACAACTTAC AATTACAGAT CGAGCTCACT CTAACAAGAA TATTTTCTAT TCTTTTAGAT 
GATGGTACCG CAAATAACTC GAGTTCTGAA AATAAGAACA AGCCATCAAT AATAAAGGAA 
CTTCTAATTG AGCAAATATC CATCTTATGG ACTAGGTCGC CATCTTTTTT TACTTCTACT 
TTTATCAATT TCGATTGTAA TCTCGATAGG GCAGACGTTT CCATAAACTT TTTGAAGGCT 
TTGACTAAAT TGGCCTTACC AGAATCCGCC TTAACTACCA CAGAAAGTGT ACCACCCATT 
TGCCTTGAGG GATTGGTCTC CCTAGTCGAT GATATGTTCG ATCACATGAA GGACATTGAC 
AGAGAAGAAT TTGGCAGGCA AAAGAATGAA ATGGAAATCT TAAAAAAGAG GGACCGTAAA 
ACAGAGTTTA TTGAATGTAC CAATGCATTC AATGAAAAGC CCAAAAAGGG TATTCCGATG 
TTAATAGAAA AAGGTTTCAT TGCTTCCGAC TCCGATAAAG ATATTGCGGA GTTTCTTTTC 
AATAATAACA ACCGTATGAA TAAAAAAACA ATCGGTTTGC TACTTTGCCA TCCGGACAAA 
GTAAGCTTGT TGAATGAATA TATTCGTTTG TTTGATTTTT CAGGGTTAAG GGTCGATGAA 
GCTATTAGAA TTTTGTTGAC GAAATTTAGG TTGCCTGGTG AATCGCAACA AATTGAAAGA 
ATCATCGAAG CCTTCTCGTC TGCGTATTGT GAAAATCAAG ATTACGATCC ATCCAAAATC 
AGTGACAACG CGGAGGATGA CATTTCTACT GTTCAACCAG ACGCTGATTC TGTTTTCATT 
TTAAGTTATT CAATTATTAT GTTGAACACT GACCTACATA ACCCTCAAGT GAAGGAACAC 
ATGTCATTTG AAGATTACTC TGGTAACTTA AAGGGATGCT GTAATCACAA AGACTTCCCA 
TTCTGGTATT TGGATAGAAT TTACTGTTCA ATCAGAGATA AAGAAATTGT TATGCCTGAA 
GAGCACCACG GCAACGAAAA GTGGTTTGAA GATGCTTGGA ATAACTTGAT ATCTTCAACT 
ACTGTTATAA CTGAAATAAA AAAAGACACA CAATCTGTCA TGGATAAATT AACACCCTTG 
GAGCTTTTGA ACTTTGATAG AGCAATTTTT AAACAAGTTG GCCCAAGTAT TGTCAGTACT 
TTATTCAACA TTTACGTAGT TGCATCTGAT GACCATATAT CTACCAGAAT GATAACAAGT 
TTGGACAAAT GTTCCTATAT TTCCGCATTT TTTGACTTCA AAGATCTCTT TAATGATATA 
CTAAACTCCA TTGCTAAGGG CACTACTTTG ATTAATTCAA GCCATGACGA TGAACTTTCA 
ACTTTAGCTT TTGAATATGG CCCAATGCCA CTGGTGCAAA TTAAATTCGA AGACACTAAC 
ACTGAGATCC CGGTTAGTAC AGATGCTGTT AGATTTGGTA GATCATTTAA GGGTCAACTA 
AATACAGTTG TTTTTTTCCG GATTATTCGC AGGAACAAAG ATCCTAAAAT TTTCTCCAAG 
GAATTATGGT TAAACATTGT TAATATTATA CTAACATTGT ACGAAGACTT GATTTTGTCT 
CCTGATATTT TCCCTGATTT ACAAAAAAGA CTGAAATTAA GCAACTTGCC TAAGCCATCT 
CCTGAAATTT CTATTAACAA GAGCAAAGAA AGCAAAGGTC TCTTATCAAC ATTTGCTTCT 
TATTTAAAAG GTGATGAAGA ACCCACAGAA GAGGAAATCA AATCCTCAAA AAAAGCGATG 
GAGTGCATAA AGTCGAGTAA TATTGCCGCC TCTGTCTTTG GAAATGAATC AAATATAACA 
GCGGATTTAA TAAAAACTTT ACTAGACTCC GCCAAAACTG AGAAAAACGC AGATAATTCC 
AGGTATTTTG AAGCAGAACT TTTATTTATC ATCGAATTGA CTATTGCATT ATTTCTATTT 
TGCAAAGAGG AGAAAGAATT AGGAAAGTTC ATACTTCAAA AAGTTTTCCA ACTTTCTCAC 
ACGAAAGGCC TCACGAAAAG GACTGTTCGT AGAATGCTAA CATACAAAAT TTTGTTAATT 
TCGTTATGTG CGGATCAGAC GGAGTACTTG TCCAAATTAA TAAACGATGA GCTGTTAAAA 
AAGGGGGATA TTTTTACCCA AAAATTTTTT GCAACTAATC AAGGTAAGGA ATTTTTGAAG 
AGACTATTTT CATTGACCGA ATCAGAGTTT TATAGAGGAT TTTTACTAGG AAATGAGAAT 
TTTTGGAAAT TTTTAAGAAA AGTTACAGCA ATGAAAGAGC AGAGCGAGAG CATTTTTGAA 
TATTTAAATG AATCGATCAA GACAGACAGC AATATTTTGA CAAATGAGAA CTTCATGTGG 
GTCCTAGGAC TATTAGATGA AATTTCATCA ATGGGTGCCG TTGGAAATCA CTGGGAAATA 
GAATACAAGA AATTGACAGA AAGTGGTCAT AAAATTGATA AGGAGAATCC ATACAAGAAA 
TCGATCGAAT TATCATTGAA ATCCATTCAA CTAACATCAC ACTTGCTGGA AGATAATAAC 
GATCTGCGTA AAAACGAGAT ATTCGCTATT ATTCAAGCTT TGGCACATCA ATGCATCAAT 
CCGTGTAAGC AGATAAGTGA ATTTGCAGTG GTAACGCTAG AGCAGACGCT CATCAATAAA 
ATCGAAATTC CAACTAATGA GATGGAATCG GTAGAAGAAT TAATTGAGGG CGGATTACTA 
CCGTTGCTAA ATTCGAGTGA AACACAGGAA GACCAGAAAA TCCTCATTTC ATCCATATTA 
ACAATAATTT CAAATGTTTA TTTGCATTAT TTGAAACTAG GGAAGACAAG CAACGAAACG 
TTTTTGAAAA TTTTGAGTAT TTTCAATAAA TTTGTAGAGG ACTCAGATAT TGAAAAAAAG 
CTACAGCAAT TAATACTTGA TAAGAAGAGT ATTGAGAAGG GCAACGGTTC ATCATCTCAT 
GGATCTGCAC ATGAACAAAC ACCAGAGTCA AACGACGTTG AAATTGAGGC TACTGCGCCA 
ATTGATGACA ATACAGACGA TGATAACAAA CCGAAGTTAT CTGATGTAGA AAAGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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GEA2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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