AFI1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following AFI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi03431 NM_001183548.1
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Saccharomyces cerevisiae S288C Afi1p (AFI1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi03431
    Clone ID Related Accession (Same CDS sequence) NM_001183548.1
    Accession Version NM_001183548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product Afi1p
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGCGAA GAGAACTAAA TAACTCTATC AGCAACAGGT CGATAGAAAA TGAATCCTTT 
    CCTTTTGAAA GGCCGAATGT CAGCTATATA ATATCCGCAG AATTTGATAA TAAATTAGGT
    CCTATTTTGA AGCACCAATA CCCGAAAGAT ATTCCAGGTT TCAATCAGTT CTCACATGAG
    CAACGCAACG GCAATACGTC GGTGTCTATG AACTTGGCAA GTTTAATGAT ACCGAGTAGC
    ATAGAGAGAA ATCCCGGCAA ACAAGATATT ACAGTGTTTA CCCTTTATTA CAATAAGTTT
    ACTCAAAACT ATCAACTCTT TCCTGTGCCA AAAGACCCTC GTTTCAGTTT CAATCTGCAT
    CATCGTGAAC AGTCAGATGG TAGTGTTACC AATAGCATAT ATTACGATGC CGAAAATCAT
    CAAGATGCAA AAAATAATAG ATACACTATT GTCTTGGAAG ATGATGAGCT CGAATGCCAG
    GAAGTTCAGA ATAATCAGAA AGCCATCGAC AACGAACCTT TATTTTTCAT AAATGTCGCC
    AACACAGTAT TAGACACCAC GAACGATAGA GGGGCAGTAA TCAAGTCGAT TGCCATCGGA
    ACACCATTAA AAACCTTCTT TGCATTCAAA AATATTATTG TATTAGTGTT AGACTTGTAT
    ATGAAAGCTC CCACTCAAGC TGCTGCTACA GACATACTGC TTGATTGCTT TAATATGCTA
    AACAGTATTG ACCTTACATT GATTAATGAC ATACATTCCA AAAGTAGTAT ACAAGAAGTG
    CTACACTCTA TCCATGACGA ATCTATCATT ACCAAAGTAT TTTTAGACCC GGATTCCACT
    TTAAAGAAAC TCTTTTGCAT CAATGGATTT GACACTAAGG ACAAGTATGG TAACATTGTG
    ACTTTTCATG ACCAACTCAT TCAATATCAT TTTACAAGAT TTCAGCCAAA GACGCTCCCG
    CCATTTTTGT TAAAAATCCC ATTGCAATTT AATATGATTA GAAGAGAGCC TATTTATATT
    GAAAATGATT ATAATGAACT AGTTTTAAAA TTTTTGGATA AATTTGTCCC TTACCTTCTT
    AAAGCCGGAC AAAAAGTTAA TGCATGGAAG TTAGTCATCA ATTCAACTAA ACTGAGTAAA
    GAAGACCTAT GCGCTTTTAT TTTGTCATTA GCCAACATAA CGGCAACTTA TGCTAGCGAT
    CCACAATCAT ACTTCAAAGG AAATGCTGCG TTGATTTTTC CCTATATGGA CATTTCTCTT
    GTTGACGGTT TGCGGGCGTA TGTAGCATCG AATAGTGATT TTGTCGGATG CTTTGCAATT
    ATCGGAACTG CCAATCCAAT TTTCCGATAC CAGTTAGATA TCTGGGATTA TTACTACGAT
    GTGGATGAGG GAGTGTTTTA TGAAAATAAT AGTCCAGAAA AAGAAAAGCC AGATACAGTG
    GCAGAAGTGA AGATTGGGCC TAACCCTTTG AGAAAGATAT TCAATAGGCC TCATTTCTCA
    ACCAACGCAG TCAACGAAAG TCAGGTAAAC TTGGGCCAGA AGCTCTTTTC TCTTCTTATC
    GACGAATATC ATGATAGTGA TACTATCATG AGCGTTTTGA GAAGGCTGAA TGTTCTTCAG
    TTGGAAAACC TTCTGGACGC TTTGAAAAGA CGAGAAATTC CACCTAATAT AGCACTAAAA
    GATGAATATA TCATGTTTTA TAAAGATTTT TTCATTTTCC CGGAGTTCTT TGATTATTTT
    ACACTGCATA GTATTGAACT ATTGTCTAAC TTGGATAATT GTTTATTTAG CTTAGGAAAC
    ACATGCCAGT TATTTTCCAC AGAGCAAATA TATTCCCAAT TAAGCCAAAT ATTGGACATT
    GTTAAAGAGC TGTTTCGCAT GGTATCGGTA AGCAGGACAA ACATTGAGAA GTTTTTAAAC
    GCTTGTTTGA ACTATTCTCC GTTCAAAATA TTACCCACAG CTCAGTTACA CGGTGATAAT
    ATTTCTAGGT GGAGTTTTGA AAGCGAAGTG CGTCAGGGTT TCGATAATTT TAACAGCTAT
    ATGGGCATCG AAAAAGATCC CCACGGTGTT ATAGTATCAG CAATAGATCT TTTTACTCAA
    ATTTACAGTT TTGATATCTT GGCGTTTTTC CTCACATTTA TTACAAAAGA AAGTGGGCAG
    GATTTGCCCT TCACAAAATC TCTGTCCAGG AGAAGAACGT ATTTGACCAG AATTGCTCAA
    TCTTCTTCAT TGAGACAGTT TTTACAACTG AGTACACGCC CTAATATAAG GATTCTGGGA
    GGTAATGGGC AGGGTACTGG AAATTCCAAT TATCCCGAAT TTACAAACGC ATCCTCAGTC
    ATCTCACCCA AGCTAAGGGC TTCTCCTCTA CTAGAAAGAA GGGCGTCAAA AATCTGCTAT
    GCTATTACGA AATTACTCTA TAGGTTAGAA TGTCATCCCA TAGGTATGGC CCTTTTAAAG
    AAGTATCTCC ACAATCAATT GCGAGAAGCC TATCTAGAAT CAAAACGACA CTTTATTTCC
    AAAAAGGGAG ATTCGACCAA CACTTCCTCT ACCATTGCAT CATCATCTTT CGCTGGCGCA
    TCTGTTCCTC TATCTTCAAA CGAATCAGGA ATGCTAAACG GCTTGAAGCA AATTAACGAA
    CAACAAGAAT CTACATTAGA AACCACTCAA AAGGAAGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_014772.1
    CDS1..2682
    Translation

    Target ORF information:

    RefSeq Version NM_001183548.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C Afi1p (AFI1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001183548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTTGCGAA GAGAACTAAA TAACTCTATC AGCAACAGGT CGATAGAAAA TGAATCCTTT 
    CCTTTTGAAA GGCCGAATGT CAGCTATATA ATATCCGCAG AATTTGATAA TAAATTAGGT
    CCTATTTTGA AGCACCAATA CCCGAAAGAT ATTCCAGGTT TCAATCAGTT CTCACATGAG
    CAACGCAACG GCAATACGTC GGTGTCTATG AACTTGGCAA GTTTAATGAT ACCGAGTAGC
    ATAGAGAGAA ATCCCGGCAA ACAAGATATT ACAGTGTTTA CCCTTTATTA CAATAAGTTT
    ACTCAAAACT ATCAACTCTT TCCTGTGCCA AAAGACCCTC GTTTCAGTTT CAATCTGCAT
    CATCGTGAAC AGTCAGATGG TAGTGTTACC AATAGCATAT ATTACGATGC CGAAAATCAT
    CAAGATGCAA AAAATAATAG ATACACTATT GTCTTGGAAG ATGATGAGCT CGAATGCCAG
    GAAGTTCAGA ATAATCAGAA AGCCATCGAC AACGAACCTT TATTTTTCAT AAATGTCGCC
    AACACAGTAT TAGACACCAC GAACGATAGA GGGGCAGTAA TCAAGTCGAT TGCCATCGGA
    ACACCATTAA AAACCTTCTT TGCATTCAAA AATATTATTG TATTAGTGTT AGACTTGTAT
    ATGAAAGCTC CCACTCAAGC TGCTGCTACA GACATACTGC TTGATTGCTT TAATATGCTA
    AACAGTATTG ACCTTACATT GATTAATGAC ATACATTCCA AAAGTAGTAT ACAAGAAGTG
    CTACACTCTA TCCATGACGA ATCTATCATT ACCAAAGTAT TTTTAGACCC GGATTCCACT
    TTAAAGAAAC TCTTTTGCAT CAATGGATTT GACACTAAGG ACAAGTATGG TAACATTGTG
    ACTTTTCATG ACCAACTCAT TCAATATCAT TTTACAAGAT TTCAGCCAAA GACGCTCCCG
    CCATTTTTGT TAAAAATCCC ATTGCAATTT AATATGATTA GAAGAGAGCC TATTTATATT
    GAAAATGATT ATAATGAACT AGTTTTAAAA TTTTTGGATA AATTTGTCCC TTACCTTCTT
    AAAGCCGGAC AAAAAGTTAA TGCATGGAAG TTAGTCATCA ATTCAACTAA ACTGAGTAAA
    GAAGACCTAT GCGCTTTTAT TTTGTCATTA GCCAACATAA CGGCAACTTA TGCTAGCGAT
    CCACAATCAT ACTTCAAAGG AAATGCTGCG TTGATTTTTC CCTATATGGA CATTTCTCTT
    GTTGACGGTT TGCGGGCGTA TGTAGCATCG AATAGTGATT TTGTCGGATG CTTTGCAATT
    ATCGGAACTG CCAATCCAAT TTTCCGATAC CAGTTAGATA TCTGGGATTA TTACTACGAT
    GTGGATGAGG GAGTGTTTTA TGAAAATAAT AGTCCAGAAA AAGAAAAGCC AGATACAGTG
    GCAGAAGTGA AGATTGGGCC TAACCCTTTG AGAAAGATAT TCAATAGGCC TCATTTCTCA
    ACCAACGCAG TCAACGAAAG TCAGGTAAAC TTGGGCCAGA AGCTCTTTTC TCTTCTTATC
    GACGAATATC ATGATAGTGA TACTATCATG AGCGTTTTGA GAAGGCTGAA TGTTCTTCAG
    TTGGAAAACC TTCTGGACGC TTTGAAAAGA CGAGAAATTC CACCTAATAT AGCACTAAAA
    GATGAATATA TCATGTTTTA TAAAGATTTT TTCATTTTCC CGGAGTTCTT TGATTATTTT
    ACACTGCATA GTATTGAACT ATTGTCTAAC TTGGATAATT GTTTATTTAG CTTAGGAAAC
    ACATGCCAGT TATTTTCCAC AGAGCAAATA TATTCCCAAT TAAGCCAAAT ATTGGACATT
    GTTAAAGAGC TGTTTCGCAT GGTATCGGTA AGCAGGACAA ACATTGAGAA GTTTTTAAAC
    GCTTGTTTGA ACTATTCTCC GTTCAAAATA TTACCCACAG CTCAGTTACA CGGTGATAAT
    ATTTCTAGGT GGAGTTTTGA AAGCGAAGTG CGTCAGGGTT TCGATAATTT TAACAGCTAT
    ATGGGCATCG AAAAAGATCC CCACGGTGTT ATAGTATCAG CAATAGATCT TTTTACTCAA
    ATTTACAGTT TTGATATCTT GGCGTTTTTC CTCACATTTA TTACAAAAGA AAGTGGGCAG
    GATTTGCCCT TCACAAAATC TCTGTCCAGG AGAAGAACGT ATTTGACCAG AATTGCTCAA
    TCTTCTTCAT TGAGACAGTT TTTACAACTG AGTACACGCC CTAATATAAG GATTCTGGGA
    GGTAATGGGC AGGGTACTGG AAATTCCAAT TATCCCGAAT TTACAAACGC ATCCTCAGTC
    ATCTCACCCA AGCTAAGGGC TTCTCCTCTA CTAGAAAGAA GGGCGTCAAA AATCTGCTAT
    GCTATTACGA AATTACTCTA TAGGTTAGAA TGTCATCCCA TAGGTATGGC CCTTTTAAAG
    AAGTATCTCC ACAATCAATT GCGAGAAGCC TATCTAGAAT CAAAACGACA CTTTATTTCC
    AAAAAGGGAG ATTCGACCAA CACTTCCTCT ACCATTGCAT CATCATCTTT CGCTGGCGCA
    TCTGTTCCTC TATCTTCAAA CGAATCAGGA ATGCTAAACG GCTTGAAGCA AATTAACGAA
    CAACAAGAAT CTACATTAGA AACCACTCAA AAGGAAGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
    Nature387(6632 Suppl)98-102(1997 May)
    Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG