SAF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following SAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi01975 NM_001178628.3
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Saccharomyces cerevisiae S288C SCF ubiquitin ligase complex subunit SAF1 (SAF1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi01975
    Clone ID Related Accession (Same CDS sequence) NM_001178628.3
    Accession Version NM_001178628.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product SCF ubiquitin ligase complex subunit SAF1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Jul 30, 2012 this sequence version replaced NM_001178628.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGTGAAG TTGAGAGCAG AGAAAAGGAG CCTGACGCAG GATTGTCCCC AGATATCGTT 
    CAAGCTACGC TACCATTTTT AAGCAGTGAT GATATTAAGA ACCTCTCGCA GACAAATAAG
    TATTATAATA CATTACTAGA TTTTGACCAC TCTAAAATTT TGTGGCATGA ACTATTTCAT
    AAGGCATTTG GGACATTAAA GACAAATGAC GAGCCTTTCC AGGGTCGAAA TTCGGCGGAA
    TTTAAGACCT GCACAGAGAC GATTTTAAGA GAAGCATTCC CCACCTTGTC ATGGCAGGAA
    GTATACCAAT TACGGGCATA TGATGCAAAG TTTTATAGTT GGGGTTATTT AAAGCATGGA
    AGGTTGGGCT ATACCGCGAG TTCCAATAAT GAACTTGTCG CCACTTCACT AAACGGGCCA
    AGTCCGCGGT TCAAATATGG TGTCAATACC CCCACTGAAG TTCCTTGGTT TAACAGTAGG
    ACAACATCTA GAACTAGTAA CTTCACTCCT TCAGAGGACC CGTTAAGCGC CATCAAGAAG
    GATGGAGACG AAATCATAGC TCAAGTATCT AGTGGTGGTT TTTCTTTCCA AATATTGACT
    GAGTCAGGTA ACCTTTACAG CAGCGGATCC ACTTTTTCTG GAGGGTTAAA AGGACCAGGT
    CCCAGTGGTT CACAGCATGA TTATAATCCC TTCAGAGAAA TGATTCACAA TATGGAAAGA
    TCGTATCCGC GGATCACTAG CCGCAGTAAT GGGAGTACTG TCAATACCAC GGGTACATTT
    TCTGGAAGGA GAATGAGCGG TAGCCATCCA TCTACTGCTT ATGAACCAGG AAATGCAACT
    ACAGCTCAGC ATATTACCAT AGATAGCGGA GGTGCGCCTG CAGCCTCTCC CGGAGGGAGC
    CATAGTGGTG TCCCTAGAAC TACAATGCCT TCAATGGGGC CGCATGAGAA TATTTACAGC
    CAGATAGAAA TGCTGGAACG AAGTGCTAAC AAGGCTGTAC CAGGAAATAA TCATATCAGG
    AGAATGTTCG CAAGAAATTC TTTCCCATTA TATAGTGGTA GGGATGAAAA TTTAGGATCA
    TTTAATGATA TTCAATTTGT CGCGGTAAGC TCTGGGAGAA GTCATTTCCT AGCAATGGAT
    ACAGATAACA ATATTTATTC TTGGGACTCA ACGGAATCTG ACCAAGGTGT CAAAATTGAG
    TTTGCTAACC TACCTAGTCG TGCTACGAAT CCTATTTTGA AAATTGCTAG TGGTTGGAAT
    TTCAATTGTT GCTATATATA TAAGGTCGGG TTGGTTGCAT GGAAAGAAAG AGATGCTATT
    CAAAAGGGCG AGAGCTTTGC TTTTGCAAAA TACGAGATTG TCCCGAATAC GAATGATGTT
    AATGGAGACT CCAGGATTGT CGACTTTGCA TGTTTACAAG ATAACTGCGT TTTCTTCATT
    AATAACAACG GCGATAAACT ATGGAAGTAC CACAATGGAC TGAACCAGAT AGTAGATTTA
    AATATTGTCG GAAAACTGTG CAAAATTAAC GCCTGTTTTG CTTCATTAGT CCTCTTCACC
    GATACTCACT GTTATACTTT GAAAGTAACA AATGGAGATG TCGATAAAGA TAGTTTAACG
    GAGCTAGACA TCAATGAAAA TGTTATCTCC GTTGCAAGTG GTGACTATCA CACGGTGGCA
    CTAACCGAAC GCGGTCATTT GTATTCATGG GGTATTGAAA GTCAGGATTG TGGGTGCTTA
    GGATTGGGCC CTTCTGAAAA AATAGTTAAC GAGCTACATA TTGGCAATTG GGAAGGGCAA
    AGAAACATTA GGGTCGTTAA GCCCACGAAA ATAGAACTAC CTGAAGATTA TATTTGTGTC
    AGTGTAACTG CTGGTGGCTG GCAAACCGGT GCATTAATCA TCAAGAAGCA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_009839.4
    CDS1..1914
    Translation

    Target ORF information:

    RefSeq Version NM_001178628.3
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C SCF ubiquitin ligase complex subunit SAF1 (SAF1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001178628.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGTGAAG TTGAGAGCAG AGAAAAGGAG CCTGACGCAG GATTGTCCCC AGATATCGTT 
    CAAGCTACGC TACCATTTTT AAGCAGTGAT GATATTAAGA ACCTCTCGCA GACAAATAAG
    TATTATAATA CATTACTAGA TTTTGACCAC TCTAAAATTT TGTGGCATGA ACTATTTCAT
    AAGGCATTTG GGACATTAAA GACAAATGAC GAGCCTTTCC AGGGTCGAAA TTCGGCGGAA
    TTTAAGACCT GCACAGAGAC GATTTTAAGA GAAGCATTCC CCACCTTGTC ATGGCAGGAA
    GTATACCAAT TACGGGCATA TGATGCAAAG TTTTATAGTT GGGGTTATTT AAAGCATGGA
    AGGTTGGGCT ATACCGCGAG TTCCAATAAT GAACTTGTCG CCACTTCACT AAACGGGCCA
    AGTCCGCGGT TCAAATATGG TGTCAATACC CCCACTGAAG TTCCTTGGTT TAACAGTAGG
    ACAACATCTA GAACTAGTAA CTTCACTCCT TCAGAGGACC CGTTAAGCGC CATCAAGAAG
    GATGGAGACG AAATCATAGC TCAAGTATCT AGTGGTGGTT TTTCTTTCCA AATATTGACT
    GAGTCAGGTA ACCTTTACAG CAGCGGATCC ACTTTTTCTG GAGGGTTAAA AGGACCAGGT
    CCCAGTGGTT CACAGCATGA TTATAATCCC TTCAGAGAAA TGATTCACAA TATGGAAAGA
    TCGTATCCGC GGATCACTAG CCGCAGTAAT GGGAGTACTG TCAATACCAC GGGTACATTT
    TCTGGAAGGA GAATGAGCGG TAGCCATCCA TCTACTGCTT ATGAACCAGG AAATGCAACT
    ACAGCTCAGC ATATTACCAT AGATAGCGGA GGTGCGCCTG CAGCCTCTCC CGGAGGGAGC
    CATAGTGGTG TCCCTAGAAC TACAATGCCT TCAATGGGGC CGCATGAGAA TATTTACAGC
    CAGATAGAAA TGCTGGAACG AAGTGCTAAC AAGGCTGTAC CAGGAAATAA TCATATCAGG
    AGAATGTTCG CAAGAAATTC TTTCCCATTA TATAGTGGTA GGGATGAAAA TTTAGGATCA
    TTTAATGATA TTCAATTTGT CGCGGTAAGC TCTGGGAGAA GTCATTTCCT AGCAATGGAT
    ACAGATAACA ATATTTATTC TTGGGACTCA ACGGAATCTG ACCAAGGTGT CAAAATTGAG
    TTTGCTAACC TACCTAGTCG TGCTACGAAT CCTATTTTGA AAATTGCTAG TGGTTGGAAT
    TTCAATTGTT GCTATATATA TAAGGTCGGG TTGGTTGCAT GGAAAGAAAG AGATGCTATT
    CAAAAGGGCG AGAGCTTTGC TTTTGCAAAA TACGAGATTG TCCCGAATAC GAATGATGTT
    AATGGAGACT CCAGGATTGT CGACTTTGCA TGTTTACAAG ATAACTGCGT TTTCTTCATT
    AATAACAACG GCGATAAACT ATGGAAGTAC CACAATGGAC TGAACCAGAT AGTAGATTTA
    AATATTGTCG GAAAACTGTG CAAAATTAAC GCCTGTTTTG CTTCATTAGT CCTCTTCACC
    GATACTCACT GTTATACTTT GAAAGTAACA AATGGAGATG TCGATAAAGA TAGTTTAACG
    GAGCTAGACA TCAATGAAAA TGTTATCTCC GTTGCAAGTG GTGACTATCA CACGGTGGCA
    CTAACCGAAC GCGGTCATTT GTATTCATGG GGTATTGAAA GTCAGGATTG TGGGTGCTTA
    GGATTGGGCC CTTCTGAAAA AATAGTTAAC GAGCTACATA TTGGCAATTG GGAAGGGCAA
    AGAAACATTA GGGTCGTTAA GCCCACGAAA ATAGAACTAC CTGAAGATTA TATTTGTGTC
    AGTGTAACTG CTGGTGGCTG GCAAACCGGT GCATTAATCA TCAAGAAGCA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    Complete DNA sequence of yeast chromosome II.
    The EMBO journal13(24)5795-809(1994 Dec)
    Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K