SEN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SEN1
Entrez Gene ID	851150
Full Name	putative DNA/RNA helicase SEN1
Synonyms	CIK3,NRD2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182318.3	NP_013534.3	putative DNA/RNA helicase SEN1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B05555g	XP_451783.1
Eremothecium gossypii	AGOS_AER115W	NP_984975.2
Saccharomyces cerevisiae (baker's yeast)	SEN1	NP_013534.3
Oryza sativa (rice)	Os06g0310200	NP_001057481.2
Arabidopsis thaliana (thale cress)	MAA3	NP_193292.3
Oryza sativa (rice)	Os03g0440200	NP_001050457.2
Oryza sativa (rice)	Os04g0424200	NP_001173937.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors also show that subdomain 1C (the "prong") is an essential element for 5'-3' unwinding of RNA and for Sen1-mediated transcription termination in vitro.
Title: Sen1 has unique structural features grafted on the architecture of the Upf1-like helicase family.

we identify new targets and functions of Sen1-dependent termination, including a role in repression of meiotic genes in vegetative cells. In combination with other recent whole-genome studies on termination of non-coding RNAs, our results provide promising directions for further investigation.
Title: Transcriptomes of six mutants in the Sen1 pathway reveal combinatorial control of transcription termination across the Saccharomyces cerevisiae genome.

The helicase domain (HD) is sufficient to dissociate the elongation complex (EC) in vitro. Both full-length Sen1 and its HD can translocate along single-stranded RNA and DNA in the 5 to 3 direction.
Title: Biochemical characterization of the helicase Sen1 provides new insights into the mechanisms of non-coding transcription termination.

DNA/RNA helicase Sen1 mutant causes modulation of protein-serine-threonine kinase TOR pathway and autophagy.
Title: Sen1, the homolog of human Senataxin, is critical for cell survival through regulation of redox homeostasis, mitochondrial function, and the TOR pathway in Saccharomyces cerevisiae.

Data indicate that mutant of the RNA processing machinery (RNT1), and RNA/DNA helicase SEN1 mutants that are unable to interact with Rnt1p, exhibit a flocculation phenotype.
Title: Flocculation in Saccharomyces cerevisiae is regulated by RNA/DNA helicase Sen1p.

The following SEN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00743	NM_001182318.3 Latest version!	Saccharomyces cerevisiae S288C putative DNA/RNA helicase SEN1 (SEN1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEN1 ( NM_001182318.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013534.3 Saccharomyces cerevisiae S288C putative DNA/RNA helicase SEN1 (SEN1), partial mRNA.

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CloneID	OSi00743
Clone ID Related Accession (Same CDS sequence)	NM_001182318.3
Accession Version	NM_001182318.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	putative DNA/RNA helicase SEN1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). On Jul 30, 2012 this sequence version replaced NM_001182318.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAATTCCA ACAATCCTGA TAATAATAAT AGCAACAACA TTAATAATAA TAATAAGGAT 
AAGGATATTG CCCCCAATAG CGATGTTCAG CTAGCTACCG TCTATACCAA GGCAAAGTCT 
TACATTCCTC AGATTGAACA AGTTTATCAA GGCACAAATC CAAATATTCA AGAGGCAAAA 
TTATTAGGGG AGTTATTACA AGTTCTAGCA GAAGTTCCCA AGGGTACGCA TTTATTTTGC 
GACCCCATAC TTGAACCAAT ATCAATTTTT TCCCTAACCA TTTTCTCATT CAATGAAGAA 
GCTACGGCCA CATGGCTGAA GAATCATTTT AATCCAATTC TCTCTGTTTG TGATAAATGC 
ATTCTAAATT TTGCGAGAGG GAAGTGCAAA ATGTTACAAC ATTTTGCCAT TCAAAGACAT 
GTGCCGCATG AACATGTAGC CAAGTTTAAT GACATCGTAT GCCAATGGAG GGTGGAGGCT 
GTTTTTCCTA TTCTTAGAAA TATTTCCGTT AACGATAATA CGGGAATTAA CATTACAAAT 
GAAATTGAAA CTGCAATGTA CGAGTGTCTC TGTAATCCGC ATATGTTGAG GCTTAACAAG 
CAACTTAAGG CTACTTTCGA AGCAATCTTC AAATTTTTCT ACGACACAAA GCATCGTTTG 
TTGGACGTTA CGAATCCATT AAGCATAAAA ACCTTTATAT CAGGTGTCAT ATTTTGCTGG 
TGTGAAGGTT CAAAAGAGGA AAATGAGTGG TCACGAGCTT TTCTAAAGGA TTTATACTCA 
AGAAATTTCC ATATAAATCT TTCTAACCTG ACACCGGATA TTATTGAGGA AGTTTATATC 
CATATTTTAT TCTTACAGAA TCCGGCAAAC TGGACAGAGA TTGTGGTATC TCAATTCTGG 
TCGAGATTAC TACCGGTTTT CAATTTATTT GATAAAGACG TCTTCATAGA GTACTTTCAG 
GTCCCAAAAA ATGTGGAATC TTTAAAAAAA ACATTCAAAT TTCCGTTAGA GCCAATTTTT 
AAAATGTGGT ACAATCACTT AAGCAAATCA TATCATGATA AGCCACTAGA CTTTTTGTTA 
AGAGGATTAA CAATGTTCTT GAATAAATTT GGCTCCGAGT TTTGGTCCAA AATTGAACCC 
TTCACATTCC ATAGCATTTT GGATATAATT TTCAATAGAG ATTCATTCCC AATCAAACTA 
ATCAAAATTC AAGACAATCC AATTGTTGAG CATCAAACAG AAGTTTACTT TCAACTTACC 
GGTTCTGTAA CCGATTTACT TTCTTGGACC TTACCATTTT ATCATGCACT GTCACCATCC 
AAAAGAATTC AAATGGTTAG AAAGGTCTCA ATGGCGTTCC TGCGTATAAT AGCCAATTAT 
CCATCCCTAA AATCCATTCC CAAAGCTTGT TTAATGAATT CAGCAACTGC CTTGTTGAGG 
GCTGTGTTGA CTATTAAGGA AAATGAAAGG GCAATGCTTT ATAAGAATGA TGAATTTGAA 
ACAGTACTGT TGACTAAAAC AGATTCTAGA GCTTTGCTCA ACAATCCTTT GATCCAAGAC 
ATAATAATTA GATCAGCTTC GAATCCTAAC GACTTTTATC CTGGGCTAGG TGCTGCTTCT 
GCATCGGTGG CCACATCCAC GATGATGGTA TTGGCAGAAT GTATCGATTT TGATATACTG 
TTATTATGTC ATCGAACGTT TAAGCTTTAT TCAGGTAAAC CAATTAGCGA AATTCCAATT 
TCAACCAACG TCTTGGAAAA TGTTACAAAT AAAATTGACC TGAGATCTTT TCATGACGGT 
CCATTGCTGG CCAAACAGCT GCTAGTATCT TTAAAAAATA TTAATGGTCT TTTGATAGTA 
CCGTCAAATA CTGCCGTAGC TGAGGCACAC AACGCATTGA ATCAGAAGTT TTTGCTGCTA 
TCAACGAGAT TAATGGAAAA ATTTGCGGAT ATTTTGCCCG GTCAACTGTC TAAAATCCTT 
GCGGATGAAG ATGCATCGCA GGGTTTTTGG TCCTGCATAT TCTCCTCTGA TAAACACTTG 
TATCAAGCAG CTACTAATAT TTTATACAAT ACATTTGACG TTGAGGGTAG ACTGGAAGGT 
ATCTTAGCGA TTCTGAATAG TAATTTGACC GTTAACTTGA AGAATATAAA CGTGATGCTA 
CAAAGATTAA TCAATTGTGA ATTCTATGAG CCTTGCCCCC GTGCTGTTCG TGTTTTGATG 
GACGTAGTGA GTGCATTTGT AGACCCGATT TCTGGTGTGT TTGCTAATTT CCAAACTTTG 
AAGAGCCAAA ATACCGAAAA GGAATTTTTA AAATTCTGGG AATCATGTTG GTTATTCTTG 
GATACGATTT ATAAGTTTAC TTTGAAATGG GCTTCTAAAT ATGACTATTC TGAACTGGAA 
AATTTCACCA AAGATACTTT AGATCTAAGT CGCTCTTTGG TGGACTCCTT TAGAGAATTT 
TCAGATATTC TTCATGACCA AACGAAAAAT TTACTTCTCA ACGTTTTAGA AACCTTTAAG 
AATATGTTGT ATTGGCTGAG ATTGAGCGAC GAAGTACTTT TGGAATCTTG TGTTAGATTA 
ATTATAAGCA CGTCTGATTT AGCACATGAA AAGCATGTGA AAGTGGATGA TTCCTTGGTA 
GAAATGATGG CCAAGTACGC CTCGAAGGCA AAGAGATTTT CAAATAAGTT GACGGAGCAA 
CAGGCGAGTG AGATTCTACA AAAGGCTAAA ATATTCAATA AAGCACTCAC GGAAGAGGTC 
GCTACAGAAG CTGAAAACTA CAGAAAAGAA AAGGAATTAT CAAGACTTGG GAAGGTGATC 
GACTTAACGG ATTCTGTGCC GGCGTCACCA TCCCTCTCGC CCTCTCTGTC TTCAACCATT 
GCTAGTTCTT CTGCTGAATC CAGGGCAGAC TATTTACAAA GAAAAGCTTT ATCTTCTTCA 
ATAACTGGGA GACCAAGAGT AGCTCAGCCT AAAATAACAT CTTTTGGTAC GTTCCAATCA 
TCAGCTAATG CTAAACTGCA TCGTACAAAA CCTGTTAAGC CTTTATCCAA AATGGAATTA 
GCTAGGATGC AATTATTAAA CAACAGAGTT GTTCATCCAC CTAGTGCGCC AGCTTTCCAT 
ACAAAGAGTA GGGGTTTGTC TAACAAAAAC GACGATAGCA GTAGTGAGGA GAGTGACAAC 
GATATAGAAA GCGCTCGGGA GCTTTTTGCT ATTGCTAAGG CAAAAGGCAA AGGAATACAA 
ACTGTCGATA TTAATGGTAA GGTTGTCAAG AGACAAACTG CGGCTGAATT GGCTAAACAG 
GAGTTAGAAC ATATGAGAAA GAGATTAAAT GTTGATATGA ACCCTCTATA TGAAATCATA 
TTGCAATGGG ATTACACCAG AAATAGTGAA TATCCTGATG ATGAGCCTAT TGGTAATTAT 
TCTGACGTAA AGGATTTCTT CAACTCTCCT GCTGACTACC AAAAAGTCAT GAAGCCTTTA 
CTACTTCTAG AATCTTGGCA AGGTCTATGT TCTTCACGTG ATAGAGAGGA CTATAAACCT 
TTCAGTATTA TTGTAGGAAA TCGAACTGCT GTGTCAGATT TCTATGATGT GTATGCCTCT 
GTCGCTAAAC AAGTCATACA GGATTGTGGT ATTTCTGAAT CTGACTTAAT TGTAATGGCT 
TATTTACCCG ATTTTCGACC AGACAAAAGG TTGTCCAGTG ATGATTTCAA GAAAGCACAA 
CATACATGTT TAGCAAAGGT GAGAACATTG AAAAATACCA AAGGTGGTAA TGTTGATGTC 
ACATTACGAA TTCATAGAAA TCATTCTTTC AGTAAATTTT TGACACTAAG ATCAGAGATA 
TATTGTGTGA AGGTTATGCA AATGACGACT ATTGAAAGAG AGTATTCAAC ACTGGAAGGG 
CTGGAATACT ATGACTTAGT TGGCCAAATT CTACAGGCTA AACCATCGCC TCCCGTAAAT 
GTGGATGCGG CAGAAATTGA AACAGTTAAG AAAAGCTATA AGCTCAATAC GTCGCAGGCT 
GAGGCCATCG TTAATTCGGT ATCTAAAGAA GGTTTTTCTT TAATTCAGGG TCCACCTGGT 
ACGGGTAAAA CAAAGACTAT ACTGGGTATT ATTGGTTACT TTTTGTCTAC AAAGAATGCC 
TCATCTTCAA ATGTCATCAA AGTGCCACTA GAAAAGAACT CATCGAATAC TGAACAACTG 
TTAAAAAAGC AAAAAATTCT AATCTGTGCC CCCAGTAATG CCGCTGTGGA CGAAATCTGC 
CTCAGACTGA AGAGTGGAGT GTATGACAAG CAGGGGCATC AATTTAAACC TCAATTGGTC 
CGTGTAGGTA GGTCAGACGT TGTAAACGTT GCAATTAAGG ACCTTACTTT AGAAGAACTT 
GTGGATAAAA GGATAGGTGA AAGGAATTAC GAAATCAGAA CAGATCCGGA ACTAGAACGT 
AAATTTAATA ATGCTGTGAC TAAAAGAAGA GAATTGAGAG GAAAATTAGA CTCTGAGAGT 
GGTAATCCAG AAAGTCCAAT GTCAACCGAA GATATCAGTA AATTACAACT TAAGATTAGG 
GAGCTAAGTA AAATAATCAA TGAACTTGGC CGTGATAGGG ACGAAATGCG TGAGAAGAAT 
TCCGTAAATT ATAGGAATAG GGATTTGGAT AGAAGGAATG CACAGGCCCA TATCTTGGCG 
GTTAGTGACA TCATATGTTC GACTTTATCT GGTTCAGCCC ATGATGTACT CGCAACAATG 
GGTATTAAAT TTGATACGGT CATTATAGAT GAAGCATGTC AATGTACCGA ACTTTCTTCA 
ATTATTCCTC TCAGGTATGG AGGGAAGCGT TGTATTATGG TTGGTGATCC TAACCAACTA 
CCACCAACTG TTCTTTCAGG TGCAGCAAGC AATTTTAAAT ACAATCAGTC ACTGTTCGTT 
AGAATGGAGA AAAATAGCTC CCCATACTTG CTGGATGTTC AATACCGTAT GCATCCTTCC 
ATCAGTAAAT TCCCCTCTTC TGAATTTTAT CAGGGACGAC TAAAAGACGG CCCTGGTATG 
GATATCTTGA ACAAAAGACC CTGGCACCAG CTTGAGCCAT TAGCTCCCTA CAAATTTTTT 
GACATCATTA GTGGTAGGCA AGAACAAAAT GCGAAGACTA TGTCTTATAC AAATATGGAG 
GAAATACGTG TTGCCATTGA GTTAGTTGAC TACCTTTTCA GAAAATTCGA TAATAAAATT 
GATTTCACGG GAAAAATCGG TATAATTTCA CCTTATAGAG AGCAAATGCA AAAAATGCGT 
AAAGAGTTTG CCCGCTATTT TGGAGGCATG ATCAACAAAT CAATCGATTT CAATACAATC 
GACGGTTTCC AAGGTCAAGA AAAAGAAATC ATCTTGATAT CGTGTGTCCG TGCCGATGAT 
ACGAAATCTA GTGTCGGTTT TTTAAAGGAC TTTCGTCGTA TGAATGTGGC TCTGACTAGA 
GCCAAGACAA GTATTTGGGT TTTGGGACAT CAAAGGTCTC TGGCTAAAAG CAAGTTATGG 
AGAGATTTGA TTGAAGATGC AAAAGATAGA AGTTGTCTTG CATATGCTTG CTCGGGTTTC 
CTGGATCCCA GAAATAATAG AGCTCAAAGT ATTTTAAGAA AGTTCAATGT ACCCGTACCA 
TCAGAGCAAG AAGATGATTA TAAGCTACCC ATGGAATATA TTACGCAAGG CCCCGATGAG 
GTAAAATCCA ATAAGGACAC AAAGAAGAGA AGAGTTGTCG ATGAAGGTGA AGAGGCAGAT 
AAAGCTGTAA AGAAAAAGAA GAAAGAGAAG AAGAAGGAAA AGAAAAAGTC CAAGGCAGAT 
GATAAGAAAA AGAATAACAA GAAGGCAGAA TCGCCCAGCA CCTCATCTGG TACAAAGAAA 
AAGTCATCCA TTTTTGGTGG AATGAGTGTA CCGAGTGCCG TAGTTCCTAA AACTTTTCCT 
GATGTGGACA GCAACAAAAA GGCAGCAGCA GTTGTTGGTA AAAAGAAAAA TAACAAACAC 
GTTTGTTTTT CGGATGATGT TAGTTTCATA CCAAGAAATG ACGAACCTGA AATTAAAGTG 
ACCAGAAGTT TATCCTCTGT ATTGAAGGAA AAGCAGTTAG GTTTGAAGGA GACGAGAACA 
ATTTCTCCGC CTGAAATCAG TAATAATGAG GATGATGATG ACGAAGATGA TTATACGCCG 
TCAATCTCCG ATTCTTCATT AATGAAAAGT GAAGCAAATG GTCGTAACAA CAGGGTGGCT 
TCTCATAACC AAAATTTCAG CGCTAGTATA TACGACGATC CACAAGTTTC GCAAGCAAAG 
CAGACGCAGG TACCTGCTGC TATTACAAAA CACAGAAGTT CAAATAGTGT TTTATCCGGT 
GGTTCTTCGA GGATCCTGAC TGCTTCCGAT TACGGTGAAC CCAATCAAAA TGGACAGAAT 
GGAGCAAATA GGACCCTCTC TCAACATGTG GGTAATGCGA ATCAATACTC AACGGCTCCT 
GTGGGTACAG GCGAACTGCA TGAAACTCTG CCCGCCCATC CGCAAGACAG CTATCCTGCT 
GAGGCAGAAG ATCCATATGA TTTAAACCCC CATCCGCAAC CACAATCTTC AGCATTCAAA 
GGACCTGGTA GTGGGCCCAC AGGCACACGC AACAGTTCGA GACGGAATGC TTCATCTAGC 
CCATTTATCC CAAAAAAAAG AAAGCCTAGA TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013534.3
CDS	1..6696
Translation	MNSNNPDNNNSNNINNNNKDKDIAPNSDVQLATVYTKAKSYIPQIEQVYQGTNPNIQEAKLLGELLQVLAEVPKGTHLFCDPILEPISIFSLTIFSFNEEATATWLKNHFNPILSVCDKCILNFARGKCKMLQHFAIQRHVPHEHVAKFNDIVCQWRVEAVFPILRNISVNDNTGINITNEIETAMYECLCNPHMLRLNKQLKATFEAIFKFFYDTKHRLLDVTNPLSIKTFISGVIFCWCEGSKEENEWSRAFLKDLYSRNFHINLSNLTPDIIEEVYIHILFLQNPANWTEIVVSQFWSRLLPVFNLFDKDVFIEYFQVPKNVESLKKTFKFPLEPIFKMWYNHLSKSYHDKPLDFLLRGLTMFLNKFGSEFWSKIEPFTFHSILDIIFNRDSFPIKLIKIQDNPIVEHQTEVYFQLTGSVTDLLSWTLPFYHALSPSKRIQMVRKVSMAFLRIIANYPSLKSIPKACLMNSATALLRAVLTIKENERAMLYKNDEFETVLLTKTDSRALLNNPLIQDIIIRSASNPNDFYPGLGAASASVATSTMMVLAECIDFDILLLCHRTFKLYSGKPISEIPISTNVLENVTNKIDLRSFHDGPLLAKQLLVSLKNINGLLIVPSNTAVAEAHNALNQKFLLLSTRLMEKFADILPGQLSKILADEDASQGFWSCIFSSDKHLYQAATNILYNTFDVEGRLEGILAILNSNLTVNLKNINVMLQRLINCEFYEPCPRAVRVLMDVVSAFVDPISGVFANFQTLKSQNTEKEFLKFWESCWLFLDTIYKFTLKWASKYDYSELENFTKDTLDLSRSLVDSFREFSDILHDQTKNLLLNVLETFKNMLYWLRLSDEVLLESCVRLIISTSDLAHEKHVKVDDSLVEMMAKYASKAKRFSNKLTEQQASEILQKAKIFNKALTEEVATEAENYRKEKELSRLGKVIDLTDSVPASPSLSPSLSSTIASSSAESRADYLQRKALSSSITGRPRVAQPKITSFGTFQSSANAKLHRTKPVKPLSKMELARMQLLNNRVVHPPSAPAFHTKSRGLSNKNDDSSSEESDNDIESARELFAIAKAKGKGIQTVDINGKVVKRQTAAELAKQELEHMRKRLNVDMNPLYEIILQWDYTRNSEYPDDEPIGNYSDVKDFFNSPADYQKVMKPLLLLESWQGLCSSRDREDYKPFSIIVGNRTAVSDFYDVYASVAKQVIQDCGISESDLIVMAYLPDFRPDKRLSSDDFKKAQHTCLAKVRTLKNTKGGNVDVTLRIHRNHSFSKFLTLRSEIYCVKVMQMTTIEREYSTLEGLEYYDLVGQILQAKPSPPVNVDAAEIETVKKSYKLNTSQAEAIVNSVSKEGFSLIQGPPGTGKTKTILGIIGYFLSTKNASSSNVIKVPLEKNSSNTEQLLKKQKILICAPSNAAVDEICLRLKSGVYDKQGHQFKPQLVRVGRSDVVNVAIKDLTLEELVDKRIGERNYEIRTDPELERKFNNAVTKRRELRGKLDSESGNPESPMSTEDISKLQLKIRELSKIINELGRDRDEMREKNSVNYRNRDLDRRNAQAHILAVSDIICSTLSGSAHDVLATMGIKFDTVIIDEACQCTELSSIIPLRYGGKRCIMVGDPNQLPPTVLSGAASNFKYNQSLFVRMEKNSSPYLLDVQYRMHPSISKFPSSEFYQGRLKDGPGMDILNKRPWHQLEPLAPYKFFDIISGRQEQNAKTMSYTNMEEIRVAIELVDYLFRKFDNKIDFTGKIGIISPYREQMQKMRKEFARYFGGMINKSIDFNTIDGFQGQEKEIILISCVRADDTKSSVGFLKDFRRMNVALTRAKTSIWVLGHQRSLAKSKLWRDLIEDAKDRSCLAYACSGFLDPRNNRAQSILRKFNVPVPSEQEDDYKLPMEYITQGPDEVKSNKDTKKRRVVDEGEEADKAVKKKKKEKKKEKKKSKADDKKKNNKKAESPSTSSGTKKKSSIFGGMSVPSAVVPKTFPDVDSNKKAAAVVGKKKNNKHVCFSDDVSFIPRNDEPEIKVTRSLSSVLKEKQLGLKETRTISPPEISNNEDDDDEDDYTPSISDSSLMKSEANGRNNRVASHNQNFSASIYDDPQVSQAKQTQVPAAITKHRSSNSVLSGGSSRILTASDYGEPNQNGQNGANRTLSQHVGNANQYSTAPVGTGELHETLPAHPQDSYPAEAEDPYDLNPHPQPQSSAFKGPGSGPTGTRNSSRRNASSSPFIPKKRKPRS

Target ORF information:

RefSeq Version	NM_001182318.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C putative DNA/RNA helicase SEN1 (SEN1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182318.3

ORF Insert Sequence:

ATGAATTCCA ACAATCCTGA TAATAATAAT AGCAACAACA TTAATAATAA TAATAAGGAT 
AAGGATATTG CCCCCAATAG CGATGTTCAG CTAGCTACCG TCTATACCAA GGCAAAGTCT 
TACATTCCTC AGATTGAACA AGTTTATCAA GGCACAAATC CAAATATTCA AGAGGCAAAA 
TTATTAGGGG AGTTATTACA AGTTCTAGCA GAAGTTCCCA AGGGTACGCA TTTATTTTGC 
GACCCCATAC TTGAACCAAT ATCAATTTTT TCCCTAACCA TTTTCTCATT CAATGAAGAA 
GCTACGGCCA CATGGCTGAA GAATCATTTT AATCCAATTC TCTCTGTTTG TGATAAATGC 
ATTCTAAATT TTGCGAGAGG GAAGTGCAAA ATGTTACAAC ATTTTGCCAT TCAAAGACAT 
GTGCCGCATG AACATGTAGC CAAGTTTAAT GACATCGTAT GCCAATGGAG GGTGGAGGCT 
GTTTTTCCTA TTCTTAGAAA TATTTCCGTT AACGATAATA CGGGAATTAA CATTACAAAT 
GAAATTGAAA CTGCAATGTA CGAGTGTCTC TGTAATCCGC ATATGTTGAG GCTTAACAAG 
CAACTTAAGG CTACTTTCGA AGCAATCTTC AAATTTTTCT ACGACACAAA GCATCGTTTG 
TTGGACGTTA CGAATCCATT AAGCATAAAA ACCTTTATAT CAGGTGTCAT ATTTTGCTGG 
TGTGAAGGTT CAAAAGAGGA AAATGAGTGG TCACGAGCTT TTCTAAAGGA TTTATACTCA 
AGAAATTTCC ATATAAATCT TTCTAACCTG ACACCGGATA TTATTGAGGA AGTTTATATC 
CATATTTTAT TCTTACAGAA TCCGGCAAAC TGGACAGAGA TTGTGGTATC TCAATTCTGG 
TCGAGATTAC TACCGGTTTT CAATTTATTT GATAAAGACG TCTTCATAGA GTACTTTCAG 
GTCCCAAAAA ATGTGGAATC TTTAAAAAAA ACATTCAAAT TTCCGTTAGA GCCAATTTTT 
AAAATGTGGT ACAATCACTT AAGCAAATCA TATCATGATA AGCCACTAGA CTTTTTGTTA 
AGAGGATTAA CAATGTTCTT GAATAAATTT GGCTCCGAGT TTTGGTCCAA AATTGAACCC 
TTCACATTCC ATAGCATTTT GGATATAATT TTCAATAGAG ATTCATTCCC AATCAAACTA 
ATCAAAATTC AAGACAATCC AATTGTTGAG CATCAAACAG AAGTTTACTT TCAACTTACC 
GGTTCTGTAA CCGATTTACT TTCTTGGACC TTACCATTTT ATCATGCACT GTCACCATCC 
AAAAGAATTC AAATGGTTAG AAAGGTCTCA ATGGCGTTCC TGCGTATAAT AGCCAATTAT 
CCATCCCTAA AATCCATTCC CAAAGCTTGT TTAATGAATT CAGCAACTGC CTTGTTGAGG 
GCTGTGTTGA CTATTAAGGA AAATGAAAGG GCAATGCTTT ATAAGAATGA TGAATTTGAA 
ACAGTACTGT TGACTAAAAC AGATTCTAGA GCTTTGCTCA ACAATCCTTT GATCCAAGAC 
ATAATAATTA GATCAGCTTC GAATCCTAAC GACTTTTATC CTGGGCTAGG TGCTGCTTCT 
GCATCGGTGG CCACATCCAC GATGATGGTA TTGGCAGAAT GTATCGATTT TGATATACTG 
TTATTATGTC ATCGAACGTT TAAGCTTTAT TCAGGTAAAC CAATTAGCGA AATTCCAATT 
TCAACCAACG TCTTGGAAAA TGTTACAAAT AAAATTGACC TGAGATCTTT TCATGACGGT 
CCATTGCTGG CCAAACAGCT GCTAGTATCT TTAAAAAATA TTAATGGTCT TTTGATAGTA 
CCGTCAAATA CTGCCGTAGC TGAGGCACAC AACGCATTGA ATCAGAAGTT TTTGCTGCTA 
TCAACGAGAT TAATGGAAAA ATTTGCGGAT ATTTTGCCCG GTCAACTGTC TAAAATCCTT 
GCGGATGAAG ATGCATCGCA GGGTTTTTGG TCCTGCATAT TCTCCTCTGA TAAACACTTG 
TATCAAGCAG CTACTAATAT TTTATACAAT ACATTTGACG TTGAGGGTAG ACTGGAAGGT 
ATCTTAGCGA TTCTGAATAG TAATTTGACC GTTAACTTGA AGAATATAAA CGTGATGCTA 
CAAAGATTAA TCAATTGTGA ATTCTATGAG CCTTGCCCCC GTGCTGTTCG TGTTTTGATG 
GACGTAGTGA GTGCATTTGT AGACCCGATT TCTGGTGTGT TTGCTAATTT CCAAACTTTG 
AAGAGCCAAA ATACCGAAAA GGAATTTTTA AAATTCTGGG AATCATGTTG GTTATTCTTG 
GATACGATTT ATAAGTTTAC TTTGAAATGG GCTTCTAAAT ATGACTATTC TGAACTGGAA 
AATTTCACCA AAGATACTTT AGATCTAAGT CGCTCTTTGG TGGACTCCTT TAGAGAATTT 
TCAGATATTC TTCATGACCA AACGAAAAAT TTACTTCTCA ACGTTTTAGA AACCTTTAAG 
AATATGTTGT ATTGGCTGAG ATTGAGCGAC GAAGTACTTT TGGAATCTTG TGTTAGATTA 
ATTATAAGCA CGTCTGATTT AGCACATGAA AAGCATGTGA AAGTGGATGA TTCCTTGGTA 
GAAATGATGG CCAAGTACGC CTCGAAGGCA AAGAGATTTT CAAATAAGTT GACGGAGCAA 
CAGGCGAGTG AGATTCTACA AAAGGCTAAA ATATTCAATA AAGCACTCAC GGAAGAGGTC 
GCTACAGAAG CTGAAAACTA CAGAAAAGAA AAGGAATTAT CAAGACTTGG GAAGGTGATC 
GACTTAACGG ATTCTGTGCC GGCGTCACCA TCCCTCTCGC CCTCTCTGTC TTCAACCATT 
GCTAGTTCTT CTGCTGAATC CAGGGCAGAC TATTTACAAA GAAAAGCTTT ATCTTCTTCA 
ATAACTGGGA GACCAAGAGT AGCTCAGCCT AAAATAACAT CTTTTGGTAC GTTCCAATCA 
TCAGCTAATG CTAAACTGCA TCGTACAAAA CCTGTTAAGC CTTTATCCAA AATGGAATTA 
GCTAGGATGC AATTATTAAA CAACAGAGTT GTTCATCCAC CTAGTGCGCC AGCTTTCCAT 
ACAAAGAGTA GGGGTTTGTC TAACAAAAAC GACGATAGCA GTAGTGAGGA GAGTGACAAC 
GATATAGAAA GCGCTCGGGA GCTTTTTGCT ATTGCTAAGG CAAAAGGCAA AGGAATACAA 
ACTGTCGATA TTAATGGTAA GGTTGTCAAG AGACAAACTG CGGCTGAATT GGCTAAACAG 
GAGTTAGAAC ATATGAGAAA GAGATTAAAT GTTGATATGA ACCCTCTATA TGAAATCATA 
TTGCAATGGG ATTACACCAG AAATAGTGAA TATCCTGATG ATGAGCCTAT TGGTAATTAT 
TCTGACGTAA AGGATTTCTT CAACTCTCCT GCTGACTACC AAAAAGTCAT GAAGCCTTTA 
CTACTTCTAG AATCTTGGCA AGGTCTATGT TCTTCACGTG ATAGAGAGGA CTATAAACCT 
TTCAGTATTA TTGTAGGAAA TCGAACTGCT GTGTCAGATT TCTATGATGT GTATGCCTCT 
GTCGCTAAAC AAGTCATACA GGATTGTGGT ATTTCTGAAT CTGACTTAAT TGTAATGGCT 
TATTTACCCG ATTTTCGACC AGACAAAAGG TTGTCCAGTG ATGATTTCAA GAAAGCACAA 
CATACATGTT TAGCAAAGGT GAGAACATTG AAAAATACCA AAGGTGGTAA TGTTGATGTC 
ACATTACGAA TTCATAGAAA TCATTCTTTC AGTAAATTTT TGACACTAAG ATCAGAGATA 
TATTGTGTGA AGGTTATGCA AATGACGACT ATTGAAAGAG AGTATTCAAC ACTGGAAGGG 
CTGGAATACT ATGACTTAGT TGGCCAAATT CTACAGGCTA AACCATCGCC TCCCGTAAAT 
GTGGATGCGG CAGAAATTGA AACAGTTAAG AAAAGCTATA AGCTCAATAC GTCGCAGGCT 
GAGGCCATCG TTAATTCGGT ATCTAAAGAA GGTTTTTCTT TAATTCAGGG TCCACCTGGT 
ACGGGTAAAA CAAAGACTAT ACTGGGTATT ATTGGTTACT TTTTGTCTAC AAAGAATGCC 
TCATCTTCAA ATGTCATCAA AGTGCCACTA GAAAAGAACT CATCGAATAC TGAACAACTG 
TTAAAAAAGC AAAAAATTCT AATCTGTGCC CCCAGTAATG CCGCTGTGGA CGAAATCTGC 
CTCAGACTGA AGAGTGGAGT GTATGACAAG CAGGGGCATC AATTTAAACC TCAATTGGTC 
CGTGTAGGTA GGTCAGACGT TGTAAACGTT GCAATTAAGG ACCTTACTTT AGAAGAACTT 
GTGGATAAAA GGATAGGTGA AAGGAATTAC GAAATCAGAA CAGATCCGGA ACTAGAACGT 
AAATTTAATA ATGCTGTGAC TAAAAGAAGA GAATTGAGAG GAAAATTAGA CTCTGAGAGT 
GGTAATCCAG AAAGTCCAAT GTCAACCGAA GATATCAGTA AATTACAACT TAAGATTAGG 
GAGCTAAGTA AAATAATCAA TGAACTTGGC CGTGATAGGG ACGAAATGCG TGAGAAGAAT 
TCCGTAAATT ATAGGAATAG GGATTTGGAT AGAAGGAATG CACAGGCCCA TATCTTGGCG 
GTTAGTGACA TCATATGTTC GACTTTATCT GGTTCAGCCC ATGATGTACT CGCAACAATG 
GGTATTAAAT TTGATACGGT CATTATAGAT GAAGCATGTC AATGTACCGA ACTTTCTTCA 
ATTATTCCTC TCAGGTATGG AGGGAAGCGT TGTATTATGG TTGGTGATCC TAACCAACTA 
CCACCAACTG TTCTTTCAGG TGCAGCAAGC AATTTTAAAT ACAATCAGTC ACTGTTCGTT 
AGAATGGAGA AAAATAGCTC CCCATACTTG CTGGATGTTC AATACCGTAT GCATCCTTCC 
ATCAGTAAAT TCCCCTCTTC TGAATTTTAT CAGGGACGAC TAAAAGACGG CCCTGGTATG 
GATATCTTGA ACAAAAGACC CTGGCACCAG CTTGAGCCAT TAGCTCCCTA CAAATTTTTT 
GACATCATTA GTGGTAGGCA AGAACAAAAT GCGAAGACTA TGTCTTATAC AAATATGGAG 
GAAATACGTG TTGCCATTGA GTTAGTTGAC TACCTTTTCA GAAAATTCGA TAATAAAATT 
GATTTCACGG GAAAAATCGG TATAATTTCA CCTTATAGAG AGCAAATGCA AAAAATGCGT 
AAAGAGTTTG CCCGCTATTT TGGAGGCATG ATCAACAAAT CAATCGATTT CAATACAATC 
GACGGTTTCC AAGGTCAAGA AAAAGAAATC ATCTTGATAT CGTGTGTCCG TGCCGATGAT 
ACGAAATCTA GTGTCGGTTT TTTAAAGGAC TTTCGTCGTA TGAATGTGGC TCTGACTAGA 
GCCAAGACAA GTATTTGGGT TTTGGGACAT CAAAGGTCTC TGGCTAAAAG CAAGTTATGG 
AGAGATTTGA TTGAAGATGC AAAAGATAGA AGTTGTCTTG CATATGCTTG CTCGGGTTTC 
CTGGATCCCA GAAATAATAG AGCTCAAAGT ATTTTAAGAA AGTTCAATGT ACCCGTACCA 
TCAGAGCAAG AAGATGATTA TAAGCTACCC ATGGAATATA TTACGCAAGG CCCCGATGAG 
GTAAAATCCA ATAAGGACAC AAAGAAGAGA AGAGTTGTCG ATGAAGGTGA AGAGGCAGAT 
AAAGCTGTAA AGAAAAAGAA GAAAGAGAAG AAGAAGGAAA AGAAAAAGTC CAAGGCAGAT 
GATAAGAAAA AGAATAACAA GAAGGCAGAA TCGCCCAGCA CCTCATCTGG TACAAAGAAA 
AAGTCATCCA TTTTTGGTGG AATGAGTGTA CCGAGTGCCG TAGTTCCTAA AACTTTTCCT 
GATGTGGACA GCAACAAAAA GGCAGCAGCA GTTGTTGGTA AAAAGAAAAA TAACAAACAC 
GTTTGTTTTT CGGATGATGT TAGTTTCATA CCAAGAAATG ACGAACCTGA AATTAAAGTG 
ACCAGAAGTT TATCCTCTGT ATTGAAGGAA AAGCAGTTAG GTTTGAAGGA GACGAGAACA 
ATTTCTCCGC CTGAAATCAG TAATAATGAG GATGATGATG ACGAAGATGA TTATACGCCG 
TCAATCTCCG ATTCTTCATT AATGAAAAGT GAAGCAAATG GTCGTAACAA CAGGGTGGCT 
TCTCATAACC AAAATTTCAG CGCTAGTATA TACGACGATC CACAAGTTTC GCAAGCAAAG 
CAGACGCAGG TACCTGCTGC TATTACAAAA CACAGAAGTT CAAATAGTGT TTTATCCGGT 
GGTTCTTCGA GGATCCTGAC TGCTTCCGAT TACGGTGAAC CCAATCAAAA TGGACAGAAT 
GGAGCAAATA GGACCCTCTC TCAACATGTG GGTAATGCGA ATCAATACTC AACGGCTCCT 
GTGGGTACAG GCGAACTGCA TGAAACTCTG CCCGCCCATC CGCAAGACAG CTATCCTGCT 
GAGGCAGAAG ATCCATATGA TTTAAACCCC CATCCGCAAC CACAATCTTC AGCATTCAAA 
GGACCTGGTA GTGGGCCCAC AGGCACACGC AACAGTTCGA GACGGAATGC TTCATCTAGC 
CCATTTATCC CAAAAAAAAG AAAGCCTAGA TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SEN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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