Gene Symbol | MID2 |
Entrez Gene ID | 851042 |
Full Name | Mid2p |
Synonyms | KAI1 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
ORF » Species Summary » Saccharomyces cerevisiae S288C » MID2 cDNA ORF clone
Gene Symbol | MID2 |
Entrez Gene ID | 851042 |
Full Name | Mid2p |
Synonyms | KAI1 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
mRNA | Protein | Name |
---|---|---|
NM_001182221.1 | NP_013436.1 | Mid2p |
The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)
Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The sensors Wsc1 and Mid2 reside in different plasma membrane compartments in Saccharomyces cerevisiae.
Title: Yeast cell wall integrity sensors form specific plasma membrane microdomains important for signalling.
The extent of the N-linked glycan and its distance from the plasma membrane affect Mid2p function, suggesting the N-glycan to be directly involved in Mid2p sensing.
Title: Protein N-glycosylation determines functionality of the Saccharomyces cerevisiae cell wall integrity sensor Mid2p.
Chimers of MID2 fused to GFP have been employed to show that intracellular and membrane distribution is only dependent on a membrane-anchored cytoplasmic tail.
Title: Functional analyses of the extra- and intracellular domains of the yeast cell wall integrity sensors Mid2 and Wsc1.
As the Mid2p amount plays a role in rgd1Delta sensitivity to low pH, Mid2p seems to act more like a molecular rheostat, controlling the level of PKC pathway activity and thus allowing phenotypical expression of RGD1 inactivation.
Title: The Rgd1p Rho GTPase-activating protein and the Mid2p cell wall sensor are required at low pH for protein kinase C pathway activation and cell survival in Saccharomyces cerevisiae.
The following MID2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSi00655 | |
Clone ID Related Accession (Same CDS sequence) | NM_001182221.1 | |
Accession Version | NM_001182221.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP |
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Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-31 | |
Organism | Saccharomyces cerevisiae S288C | |
Product | Mid2p | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. |
1 | ATGTTGTCTT TCACAACCAA GAATAGTTTC CGCTTATTAC TTTTAATACT GTCATGCATA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_013436.1 |
CDS | 1..1131 |
Translation |
Target ORF information:
Target ORF information:
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1 | ATGTTGTCTT TCACAACCAA GAATAGTTTC CGCTTATTAC TTTTAATACT GTCATGCATA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XII. |
Life with 6000 genes. |