Gene Symbol | DCN1 |
Entrez Gene ID | 850819 |
Full Name | NEDD8 ligase DCN1 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
ORF » Species Summary » Saccharomyces cerevisiae S288C » DCN1 cDNA ORF clone
Gene Symbol | DCN1 |
Entrez Gene ID | 850819 |
Full Name | NEDD8 ligase DCN1 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
mRNA | Protein | Name |
---|---|---|
NM_001182015.1 | NP_013229.1 | NEDD8 ligase DCN1 |
Pan troglodytes (chimpanzee) | DCUN1D2 | XP_509747.3 |
Canis lupus familiaris (dog) | DCUN1D2 | XP_005634202.1 |
Bos taurus (cattle) | DCUN1D2 | XP_002692045.2 |
Danio rerio (zebrafish) | dcun1d2a | NP_991101.1 |
Xenopus tropicalis (tropical clawed frog) | dcun1d2 | NP_001120037.1 |
Mus musculus (house mouse) | Dcun1d2 | NP_001019675.2 |
Eremothecium gossypii | AGOS_AGL194C | NP_986473.2 |
Oryza sativa (rice) | Os06g0233400 | NP_001057232.1 |
Homo sapiens (human) | DCUN1D2 | NP_001014305.1 |
Caenorhabditis elegans (roundworm) | dcn-1 | NP_497866.2 |
Saccharomyces cerevisiae (baker's yeast) | DCN1 | NP_013229.1 |
Rattus norvegicus (Norway rat) | Dcun1d2 | NP_001128270.1 |
Gallus gallus (chicken) | DCUN1D2 | XP_416939.4 |
Kluyveromyces lactis | KLLA0D16346g | XP_453781.1 |
Arabidopsis thaliana (thale cress) | AT3G12760 | NP_566436.1 |
The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)
Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The 1.9 A X-ray crystal structure of Dcn1 has an N-terminal ubiquitin-binding domain & a unique C-terminal domain. It does not require Cys for catalysis. It interacts with the Nedd8 E2 Ubc12 on a surface that overlaps the E1-binding site.
Title: Dcn1 functions as a scaffold-type E3 ligase for cullin neddylation.
DCN-1 is a scaffold protein in a multisubunit neddylation E3 ligase complex
Title: Structural basis for the function of DCN-1 in protein Neddylation.
Both in vivo and in vitro experiments indicate that Dcn1p does not inhibit deneddylation of Cdc53p by the COP9 signalosome, but greatly increases the kinetics of the neddylation reaction
Title: The conserved protein DCN-1/Dcn1p is required for cullin neddylation in C. elegans and S. cerevisiae.
The following DCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSi00473 | |
Clone ID Related Accession (Same CDS sequence) | NM_001182015.1 | |
Accession Version | NM_001182015.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP |
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Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-31 | |
Organism | Saccharomyces cerevisiae S288C | |
Product | NEDD8 ligase DCN1 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. |
1 | ATGAGTAATA ATAAAATAAA AAGAAAGGAT GCAAGCCCGG AACAAGAGGC AATTGAATCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_013229.1 |
CDS | 1..810 |
Translation |
Target ORF information:
Target ORF information:
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1 | ATGAGTAATA ATAAAATAAA AAGAAAGGAT GCAAGCCCGG AACAAGAGGC AATTGAATCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XII. |
Life with 6000 genes. |