Gene Symbol | LOT6 |
Entrez Gene ID | 850698 |
Full Name | flavin-dependent quinone reductase |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
ORF » Species Summary » Saccharomyces cerevisiae S288C » LOT6 cDNA ORF clone
Gene Symbol | LOT6 |
Entrez Gene ID | 850698 |
Full Name | flavin-dependent quinone reductase |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
mRNA | Protein | Name |
---|---|---|
NM_001181898.1 | NP_013111.1 | flavin-dependent quinone reductase |
Schizosaccharomyces pombe (fission yeast) | SPCC4B3.06c | NP_588084.1 |
Saccharomyces cerevisiae (baker's yeast) | LOT6 | NP_013111.1 |
Magnaporthe oryzae (rice blast fungus) | MGG_05084 | XP_003712605.1 |
The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)
Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Using hexahistidine tagged, recombinant Lot6p, the authors determined the steady-state enzyme kinetic parameters with both NADH and NADPH as electron donors; no cooperativity was observed with these substrates.
Title: The Saccharomyces cerevisiae quinone oxidoreductase Lot6p: stability, inhibition and cooperativity.
The authors propose that Lot6p is directly involved in the control of the apoptosis-like cell death in yeast.
Title: The redox-sensing quinone reductase Lot6p acts as an inducer of yeast apoptosis.
Hydride transfer, and not an enzyme conformational change, is rate-determining in the Lot6 reductive half-reaction. In the oxidative half-reaction, reduced enzyme reacted in a single phase with six quinone substrates tested.
Title: Mechanism of flavin reduction and oxidation in the redox-sensing quinone reductase Lot6p from Saccharomyces cerevisiae.
Lot6-proteasome complex can be regarded as a redox switch in which the quinone reductase acts as a sensor for oxidative stress
Title: Quinone reductase acts as a redox switch of the 20S yeast proteasome.
LOT6 is a FMN-dependent reductase with a potential role in quinone detoxification.
Title: Lot6p from Saccharomyces cerevisiae is a FMN-dependent reductase with a potential role in quinone detoxification.
The following LOT6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOT6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSi00363 | |
Clone ID Related Accession (Same CDS sequence) | NM_001181898.1 | |
Accession Version | NM_001181898.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-31 | |
Organism | Saccharomyces cerevisiae S288C | |
Product | flavin-dependent quinone reductase | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. |
1 | ATGAAAGTGG GTATTATAAT GGGTTCTGTG AGGGCAAAGA GGGTATGCCC AGAAATTGCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_013111.1 |
CDS | 1..576 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001181898.1 |
1 | ATGAAAGTGG GTATTATAAT GGGTTCTGTG AGGGCAAAGA GGGTATGCCC AGAAATTGCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XII. |
Life with 6000 genes. |