LOT6 cDNA ORF clone, Saccharomyces cerevisiae S288C

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
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Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Using hexahistidine tagged, recombinant Lot6p, the authors determined the steady-state enzyme kinetic parameters with both NADH and NADPH as electron donors; no cooperativity was observed with these substrates.
Title: The Saccharomyces cerevisiae quinone oxidoreductase Lot6p: stability, inhibition and cooperativity.

The authors propose that Lot6p is directly involved in the control of the apoptosis-like cell death in yeast.
Title: The redox-sensing quinone reductase Lot6p acts as an inducer of yeast apoptosis.

Hydride transfer, and not an enzyme conformational change, is rate-determining in the Lot6 reductive half-reaction. In the oxidative half-reaction, reduced enzyme reacted in a single phase with six quinone substrates tested.
Title: Mechanism of flavin reduction and oxidation in the redox-sensing quinone reductase Lot6p from Saccharomyces cerevisiae.

Lot6-proteasome complex can be regarded as a redox switch in which the quinone reductase acts as a sensor for oxidative stress
Title: Quinone reductase acts as a redox switch of the 20S yeast proteasome.

LOT6 is a FMN-dependent reductase with a potential role in quinone detoxification.
Title: Lot6p from Saccharomyces cerevisiae is a FMN-dependent reductase with a potential role in quinone detoxification.