HSP104 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	HSP104
Entrez Gene ID	850633
Full Name	chaperone ATPase HSP104
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181846.1	NP_013074.1	chaperone ATPase HSP104

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AGL036C	NP_986630.1
Arabidopsis thaliana (thale cress)	HSP101	NP_565083.1
Saccharomyces cerevisiae (baker's yeast)	HSP104	NP_013074.1
Neurospora crassa	NCU00104	XP_957228.1
Oryza sativa (rice)	Os05g0519700	NP_001056066.1
Schizosaccharomyces pombe (fission yeast)	SPBC16D10.08c	NP_596503.1
Magnaporthe oryzae (rice blast fungus)	MGG_06459	XP_003717107.1
Kluyveromyces lactis	KLLA0F18194g	XP_455896.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study reports cryo-electron microscopy structures at near-atomic resolution of Hsp104 in different translocation states.
Title: Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104.

curing by Hsp104 overexpression depends on both the trimming ability of the fungal Hsp104 homolog and the strength of the [PSI(+)] variant: the greater the trimming activity of the Hsp104 homolog and the weaker the variant, the greater the curing.
Title: Heat shock protein 104 (Hsp104)-mediated curing of [

The structures of Saccharomyces cerevisiae Hsp104 N-terminal domain (NTD) and Candida albicans Hsp104 NTD reveal that the yeast Hsp104 NTD may utilize a conserved putative peptide-binding groove to interact with misfolded polypeptides.
Title: Crystal structures of Hsp104 N-terminal domains from Saccharomyces cerevisiae and Candida albicans suggest the mechanism for the function of Hsp104 in dissolving prions.

Hsp31 can modulate prion status in cooperation with Hsp104 because it inhibits Sup35 aggregate formation and potentiates [PSI(+)] prion curing upon overexpression of Hsp104
Title: The small heat shock protein Hsp31 cooperates with Hsp104 to modulate Sup35 prion aggregation.

The authors show here that both Escherichia coli ClpB and Saccharomyces cerevisiae Hsp104 cooperation with their cognate Hsp70 is crucial for efficient protein disaggregation and, in contrast to earlier claims, cannot be circumvented by activating M-domain mutations.
Title: Bacterial and Yeast AAA+ Disaggregases ClpB and Hsp104 Operate through Conserved Mechanism Involving Cooperation with Hsp70.

The following HSP104 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSP104 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00306	NM_001181846.1 Latest version!	Saccharomyces cerevisiae S288C chaperone ATPase HSP104 (HSP104), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSP104 ( NM_001181846.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013074.1 Saccharomyces cerevisiae S288C chaperone ATPase HSP104 (HSP104), partial mRNA.

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CloneID	OSi00306
Clone ID Related Accession (Same CDS sequence)	NM_001181846.1
Accession Version	NM_001181846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	chaperone ATPase HSP104
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAACGACC AAACGCAATT TACAGAAAGG GCTCTAACGA TTTTGACGTT GGCTCAAAAA 
TTGGCTTCGG ATCATCAACA TCCACAATTA CAACCTATAC ATATTCTAGC TGCCTTCATT 
GAAACGCCAG AAGATGGATC AGTCCCTTAC CTACAGAATC TAATTGAGAA GGGCCGTTAC 
GACTATGATC TTTTCAAGAA AGTGGTTAAT AGAAATCTAG TAAGAATTCC TCAACAGCAA 
CCTGCACCTG CGGAGATAAC TCCAAGTTAT GCTTTGGGGA AAGTCCTTCA AGACGCTGCT 
AAGATTCAAA AACAACAGAA GGACTCATTT ATAGCGCAAG ACCATATATT GTTTGCTCTA 
TTCAATGATT CGTCTATTCA GCAAATATTT AAGGAAGCTC AAGTAGATAT TGAGGCCATC 
AAGCAACAAG CTCTTGAACT TCGTGGTAAC ACTAGAATTG ACTCTCGTGG CGCTGATACG 
AACACACCTT TGGAATATTT ATCAAAGTAC GCCATTGATA TGACTGAGCA GGCTCGTCAA 
GGTAAACTTG ACCCTGTCAT CGGCCGTGAA GAAGAAATAA GAAGCACTAT TAGAGTTTTA 
GCAAGAAGAA TTAAGTCCAA CCCATGTTTA ATTGGTGAGC CAGGTATCGG TAAGACCGCT 
ATTATTGAAG GTGTTGCTCA AAGAATCATT GACGATGACG TTCCCACTAT CTTACAAGGC 
GCTAAATTGT TCAGTCTAGA TTTGGCCGCA TTAACCGCAG GTGCTAAATA CAAAGGTGAT 
TTCGAAGAAA GATTCAAAGG TGTTTTGAAG GAAATCGAAG AATCAAAGAC TCTAATTGTG 
TTATTCATTG ATGAAATTCA CATGTTAATG GGTAATGGTA AGGACGACGC TGCTAACATC 
TTGAAGCCAG CTTTGTCCAG AGGCCAATTG AAGGTCATCG GTGCCACCAC CAATAACGAA 
TATAGATCTA TTGTGGAAAA GGATGGTGCC TTTGAAAGAA GATTCCAGAA AATTGAAGTC 
GCTGAACCAA GTGTGAGACA AACAGTGGCC ATATTGAGAG GTCTGCAACC AAAGTATGAA 
ATACATCATG GTGTAAGGAT TCTGGATAGC GCCTTAGTCA CTGCTGCTCA ATTAGCCAAG 
CGTTACTTGC CATATAGAAG ATTGCCAGAT TCTGCTTTGG ATTTAGTTGA TATTTCTTGT 
GCTGGTGTCG CCGTCGCAAG AGATTCTAAG CCAGAAGAAT TGGATTCCAA GGAACGTCAA 
TTGCAATTGA TTCAAGTAGA GATAAAAGCT CTAGAGAGAG ATGAAGATGC CGACTCCACC 
ACTAAAGATA GATTAAAGTT AGCTAGGCAG AAGGAAGCTT CATTGCAAGA AGAATTGGAA 
CCTCTAAGAC AACGTTACAA TGAAGAAAAG CATGGCCATG AAGAATTGAC ACAAGCTAAA 
AAGAAATTGG ATGAACTGGA AAACAAGGCC CTTGATGCTG AACGTAGATA TGATACTGCT 
ACCGCCGCTG ATTTAAGGTA CTTCGCCATC CCAGATATCA AAAAGCAAAT CGAAAAGCTT 
GAAGATCAGG TTGCTGAGGA AGAGAGACGT GCTGGTGCCA ACTCCATGAT CCAAAATGTG 
GTCGATTCAG ACACCATTTC TGAAACAGCT GCAAGATTGA CTGGTATCCC TGTTAAGAAG 
TTGTCAGAAT CTGAAAATGA AAAATTGATT CATATGGAAC GTGACTTATC ATCTGAAGTC 
GTGGGCCAAA TGGATGCCAT TAAAGCTGTT TCCAATGCCG TTAGATTGTC TAGATCAGGT 
TTAGCTAATC CAAGGCAACC AGCATCCTTC TTATTTTTAG GTTTGTCCGG TTCCGGTAAA 
ACTGAATTGG CTAAAAAAGT TGCTGGATTT TTGTTTAATG ATGAGGACAT GATGATCAGG 
GTCGATTGTT CTGAATTAAG CGAGAAGTAT GCGGTCTCTA AGTTGTTGGG TACCACGGCA 
GGTTATGTCG GGTACGATGA AGGTGGCTTT TTAACTAACC AACTGCAATA CAAACCATAC 
TCCGTTTTGT TATTCGATGA AGTAGAAAAG GCACATCCTG ATGTTTTGAC TGTCATGCTA 
CAAATGTTGG ATGACGGTAG AATTACTTCT GGTCAAGGTA AGACGATCGA CTGTTCCAAT 
TGTATTGTCA TCATGACTTC CAATCTAGGT GCTGAATTTA TCAATTCTCA ACAAGGATCA 
AAGATCCAAG AATCTACCAA GAATTTGGTC ATGGGTGCTG TTAGGCAACA TTTCAGACCA 
GAATTTTTGA ACAGAATTTC TAGTATAGTC ATTTTCAACA AGCTATCTAG AAAAGCTATT 
CATAAGATCG TGGATATTCG TTTGAAGGAA ATTGAAGAGA GATTCGAGCA AAATGATAAA 
CATTACAAGT TGAATTTAAC TCAAGAGGCC AAGGACTTCT TGGCCAAATA TGGTTATTCC 
GATGATATGG GTGCACGTCC ACTGAACAGG TTAATTCAAA ACGAAATTTT GAACAAACTG 
GCACTAAGGA TCTTAAAGAA TGAAATCAAG GATAAGGAAA CTGTCAATGT CGTCTTGAAG 
AAGGGTAAAT CTCGTGATGA AAATGTTCCT GAGGAAGCTG AAGAATGTCT GGAAGTTCTA 
CCAAATCACG AAGCTACTAT AGGGGCTGAC ACGTTAGGTG ATGACGATAA TGAGGACAGT 
ATGGAAATTG ATGATGACCT AGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013074.1
CDS	1..2727
Translation	MNDQTQFTERALTILTLAQKLASDHQHPQLQPIHILAAFIETPEDGSVPYLQNLIEKGRYDYDLFKKVVNRNLVRIPQQQPAPAEITPSYALGKVLQDAAKIQKQQKDSFIAQDHILFALFNDSSIQQIFKEAQVDIEAIKQQALELRGNTRIDSRGADTNTPLEYLSKYAIDMTEQARQGKLDPVIGREEEIRSTIRVLARRIKSNPCLIGEPGIGKTAIIEGVAQRIIDDDVPTILQGAKLFSLDLAALTAGAKYKGDFEERFKGVLKEIEESKTLIVLFIDEIHMLMGNGKDDAANILKPALSRGQLKVIGATTNNEYRSIVEKDGAFERRFQKIEVAEPSVRQTVAILRGLQPKYEIHHGVRILDSALVTAAQLAKRYLPYRRLPDSALDLVDISCAGVAVARDSKPEELDSKERQLQLIQVEIKALERDEDADSTTKDRLKLARQKEASLQEELEPLRQRYNEEKHGHEELTQAKKKLDELENKALDAERRYDTATAADLRYFAIPDIKKQIEKLEDQVAEEERRAGANSMIQNVVDSDTISETAARLTGIPVKKLSESENEKLIHMERDLSSEVVGQMDAIKAVSNAVRLSRSGLANPRQPASFLFLGLSGSGKTELAKKVAGFLFNDEDMMIRVDCSELSEKYAVSKLLGTTAGYVGYDEGGFLTNQLQYKPYSVLLFDEVEKAHPDVLTVMLQMLDDGRITSGQGKTIDCSNCIVIMTSNLGAEFINSQQGSKIQESTKNLVMGAVRQHFRPEFLNRISSIVIFNKLSRKAIHKIVDIRLKEIEERFEQNDKHYKLNLTQEAKDFLAKYGYSDDMGARPLNRLIQNEILNKLALRILKNEIKDKETVNVVLKKGKSRDENVPEEAEECLEVLPNHEATIGADTLGDDDNEDSMEIDDDLD

Target ORF information:

RefSeq Version	NM_001181846.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C chaperone ATPase HSP104 (HSP104), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181846.1

ORF Insert Sequence:

ATGAACGACC AAACGCAATT TACAGAAAGG GCTCTAACGA TTTTGACGTT GGCTCAAAAA 
TTGGCTTCGG ATCATCAACA TCCACAATTA CAACCTATAC ATATTCTAGC TGCCTTCATT 
GAAACGCCAG AAGATGGATC AGTCCCTTAC CTACAGAATC TAATTGAGAA GGGCCGTTAC 
GACTATGATC TTTTCAAGAA AGTGGTTAAT AGAAATCTAG TAAGAATTCC TCAACAGCAA 
CCTGCACCTG CGGAGATAAC TCCAAGTTAT GCTTTGGGGA AAGTCCTTCA AGACGCTGCT 
AAGATTCAAA AACAACAGAA GGACTCATTT ATAGCGCAAG ACCATATATT GTTTGCTCTA 
TTCAATGATT CGTCTATTCA GCAAATATTT AAGGAAGCTC AAGTAGATAT TGAGGCCATC 
AAGCAACAAG CTCTTGAACT TCGTGGTAAC ACTAGAATTG ACTCTCGTGG CGCTGATACG 
AACACACCTT TGGAATATTT ATCAAAGTAC GCCATTGATA TGACTGAGCA GGCTCGTCAA 
GGTAAACTTG ACCCTGTCAT CGGCCGTGAA GAAGAAATAA GAAGCACTAT TAGAGTTTTA 
GCAAGAAGAA TTAAGTCCAA CCCATGTTTA ATTGGTGAGC CAGGTATCGG TAAGACCGCT 
ATTATTGAAG GTGTTGCTCA AAGAATCATT GACGATGACG TTCCCACTAT CTTACAAGGC 
GCTAAATTGT TCAGTCTAGA TTTGGCCGCA TTAACCGCAG GTGCTAAATA CAAAGGTGAT 
TTCGAAGAAA GATTCAAAGG TGTTTTGAAG GAAATCGAAG AATCAAAGAC TCTAATTGTG 
TTATTCATTG ATGAAATTCA CATGTTAATG GGTAATGGTA AGGACGACGC TGCTAACATC 
TTGAAGCCAG CTTTGTCCAG AGGCCAATTG AAGGTCATCG GTGCCACCAC CAATAACGAA 
TATAGATCTA TTGTGGAAAA GGATGGTGCC TTTGAAAGAA GATTCCAGAA AATTGAAGTC 
GCTGAACCAA GTGTGAGACA AACAGTGGCC ATATTGAGAG GTCTGCAACC AAAGTATGAA 
ATACATCATG GTGTAAGGAT TCTGGATAGC GCCTTAGTCA CTGCTGCTCA ATTAGCCAAG 
CGTTACTTGC CATATAGAAG ATTGCCAGAT TCTGCTTTGG ATTTAGTTGA TATTTCTTGT 
GCTGGTGTCG CCGTCGCAAG AGATTCTAAG CCAGAAGAAT TGGATTCCAA GGAACGTCAA 
TTGCAATTGA TTCAAGTAGA GATAAAAGCT CTAGAGAGAG ATGAAGATGC CGACTCCACC 
ACTAAAGATA GATTAAAGTT AGCTAGGCAG AAGGAAGCTT CATTGCAAGA AGAATTGGAA 
CCTCTAAGAC AACGTTACAA TGAAGAAAAG CATGGCCATG AAGAATTGAC ACAAGCTAAA 
AAGAAATTGG ATGAACTGGA AAACAAGGCC CTTGATGCTG AACGTAGATA TGATACTGCT 
ACCGCCGCTG ATTTAAGGTA CTTCGCCATC CCAGATATCA AAAAGCAAAT CGAAAAGCTT 
GAAGATCAGG TTGCTGAGGA AGAGAGACGT GCTGGTGCCA ACTCCATGAT CCAAAATGTG 
GTCGATTCAG ACACCATTTC TGAAACAGCT GCAAGATTGA CTGGTATCCC TGTTAAGAAG 
TTGTCAGAAT CTGAAAATGA AAAATTGATT CATATGGAAC GTGACTTATC ATCTGAAGTC 
GTGGGCCAAA TGGATGCCAT TAAAGCTGTT TCCAATGCCG TTAGATTGTC TAGATCAGGT 
TTAGCTAATC CAAGGCAACC AGCATCCTTC TTATTTTTAG GTTTGTCCGG TTCCGGTAAA 
ACTGAATTGG CTAAAAAAGT TGCTGGATTT TTGTTTAATG ATGAGGACAT GATGATCAGG 
GTCGATTGTT CTGAATTAAG CGAGAAGTAT GCGGTCTCTA AGTTGTTGGG TACCACGGCA 
GGTTATGTCG GGTACGATGA AGGTGGCTTT TTAACTAACC AACTGCAATA CAAACCATAC 
TCCGTTTTGT TATTCGATGA AGTAGAAAAG GCACATCCTG ATGTTTTGAC TGTCATGCTA 
CAAATGTTGG ATGACGGTAG AATTACTTCT GGTCAAGGTA AGACGATCGA CTGTTCCAAT 
TGTATTGTCA TCATGACTTC CAATCTAGGT GCTGAATTTA TCAATTCTCA ACAAGGATCA 
AAGATCCAAG AATCTACCAA GAATTTGGTC ATGGGTGCTG TTAGGCAACA TTTCAGACCA 
GAATTTTTGA ACAGAATTTC TAGTATAGTC ATTTTCAACA AGCTATCTAG AAAAGCTATT 
CATAAGATCG TGGATATTCG TTTGAAGGAA ATTGAAGAGA GATTCGAGCA AAATGATAAA 
CATTACAAGT TGAATTTAAC TCAAGAGGCC AAGGACTTCT TGGCCAAATA TGGTTATTCC 
GATGATATGG GTGCACGTCC ACTGAACAGG TTAATTCAAA ACGAAATTTT GAACAAACTG 
GCACTAAGGA TCTTAAAGAA TGAAATCAAG GATAAGGAAA CTGTCAATGT CGTCTTGAAG 
AAGGGTAAAT CTCGTGATGA AAATGTTCCT GAGGAAGCTG AAGAATGTCT GGAAGTTCTA 
CCAAATCACG AAGCTACTAT AGGGGCTGAC ACGTTAGGTG ATGACGATAA TGAGGACAGT 
ATGGAAATTG ATGATGACCT AGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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HSP104 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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