SEC4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SEC4
Entrez Gene ID	850543
Full Name	Rab family GTPase SEC4
Synonyms	SRO6
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179961.1	NP_116650.1	Rab family GTPase SEC4

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	rab8a	NP_001083031.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001211	XP_321946.4
Eremothecium gossypii	AGOS_AGL021W	NP_986645.1
Schizosaccharomyces pombe (fission yeast)	ypt2	NP_594580.1
Neurospora crassa	NCU06404	XP_962242.1
Arabidopsis thaliana (thale cress)	RAB8	NP_190929.1
Magnaporthe oryzae (rice blast fungus)	MGG_06135	XP_003712007.1
Oryza sativa (rice)	Os07g0239400	NP_001059259.1
Canis lupus familiaris (dog)	RAB8A	NP_001003152.1
Bos taurus (cattle)	RAB8A	NP_001098951.1
Gallus gallus (chicken)	RAB8A	NP_001026675.1
Drosophila melanogaster (fruit fly)	Rab8	NP_001246837.1
Saccharomyces cerevisiae (baker's yeast)	SEC4	NP_116650.1
Kluyveromyces lactis	KLLA0E12079g	XP_454494.1
Oryza sativa (rice)	Os05g0461300	NP_001055761.1
Xenopus tropicalis (tropical clawed frog)	rab8a	XP_002934841.1
Homo sapiens (human)	RAB8A	NP_005361.2
Pan troglodytes (chimpanzee)	RAB8A	NP_001229300.1
Rattus norvegicus (Norway rat)	Rab8a	NP_446450.2
Caenorhabditis elegans (roundworm)	rab-8	NP_491199.2
Arabidopsis thaliana (thale cress)	AT5G59840	NP_200792.1
Mus musculus (house mouse)	Rab8a	NP_075615.2
Arabidopsis thaliana (thale cress)	RAB8A	NP_001118780.1
Oryza sativa (rice)	Os03g0819900	NP_001051716.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sec4p phosphorylation facilitates the coordination of membrane-trafficking events during cytokinesis.
Title: Cell cycle-dependent phosphorylation of Sec4p controls membrane deposition during cytokinesis.

Data show that mutations in Rab GTPase Sec4p (SEC4) disrupt actin patch assembly and proper endocytic internalization.
Title: Polarized Exocytosis Induces Compensatory Endocytosis by Sec4p-Regulated Cortical Actin Polymerization.

Authors describe the identification of a Sec4 binding site on the surface of Sro7 that is contained within a cleft created by the junction of two adjacent beta-propellers that form the core structure of Sro7.
Title: Structural basis for recognition of the Sec4 Rab GTPase by its effector, the Lgl/tomosyn homologue, Sro7.

Results show that Sec4p serine 29 forms a hydrogen bond with the nucleotide and its can form stable associations with Sec2p allowing to elucidate the structure of the Sec4p-Sec2p complex representing an early stage of the exchange reaction.
Title: New insights into the molecular mechanism of the Rab GTPase Sec4p activation.

Intracellular traffic in yeast between the Golgi and the cell surface is mediated by vesicular carriers and highly dependent on both Sro7 and Rab GTPase, Sec4.
Title: In vitro reconstitution of Rab GTPase-dependent vesicle clustering by the yeast lethal giant larvae/tomosyn homolog, Sro7.

The following SEC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00230	NM_001179961.1 Latest version!	Saccharomyces cerevisiae S288C Rab family GTPase SEC4 (SEC4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEC4 ( NM_001179961.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116650.1 Saccharomyces cerevisiae S288C Rab family GTPase SEC4 (SEC4), partial mRNA.

Price & Availability↑

CloneID	OSi00230
Clone ID Related Accession (Same CDS sequence)	NM_001179961.1
Accession Version	NM_001179961.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Rab family GTPase SEC4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAGGCT TGAGAACTGT TTCTGCTTCA TCCGGTAATG GAAAGAGCTA TGACTCTATT 
ATGAAAATTT TATTGATTGG TGATTCTGGT GTTGGGAAAT CATGTTTATT GGTTCGTTTT 
GTTGAAGACA AATTTAACCC GTCATTTATC ACCACCATTG GTATTGATTT CAAAATAAAG 
ACTGTCGATA TCAACGGTAA GAAGGTAAAG CTGCAACTTT GGGATACCGC TGGTCAAGAA 
CGTTTCCGGA CAATCACCAC AGCGTATTAT CGTGGTGCTA TGGGTATCAT TCTTGTATAT 
GATGTGACAG ACGAGAGAAC ATTTACTAAT ATCAAGCAAT GGTTTAAAAC CGTTAATGAG 
CATGCGAACG ATGAAGCACA GCTACTGTTG GTTGGTAACA AGAGCGATAT GGAGACGAGA 
GTGGTAACAG CTGATCAAGG TGAAGCCTTG GCTAAGGAGC TGGGTATACC ATTCATCGAG 
TCCAGTGCTA AGAACGATGA CAATGTCAAC GAGATTTTTT TCACCTTGGC GAAGTTAATC 
CAAGAAAAAA TCGACAGTAA CAAGCTTGTT GGCGTCGGTA ACGGTAAAGA GGGCAATATT 
AGCATCAATA GTGGGAGCGG AAACAGTTCT AAATCAAATT GCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116650.1
CDS	1..648
Translation	MSGLRTVSASSGNGKSYDSIMKILLIGDSGVGKSCLLVRFVEDKFNPSFITTIGIDFKIKTVDINGKKVKLQLWDTAGQERFRTITTAYYRGAMGIILVYDVTDERTFTNIKQWFKTVNEHANDEAQLLLVGNKSDMETRVVTADQGEALAKELGIPFIESSAKNDDNVNEIFFTLAKLIQEKIDSNKLVGVGNGKEGNISINSGSGNSSKSNCC

Target ORF information:

RefSeq Version	NM_001179961.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Rab family GTPase SEC4 (SEC4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179961.1

ORF Insert Sequence:

ATGTCAGGCT TGAGAACTGT TTCTGCTTCA TCCGGTAATG GAAAGAGCTA TGACTCTATT 
ATGAAAATTT TATTGATTGG TGATTCTGGT GTTGGGAAAT CATGTTTATT GGTTCGTTTT 
GTTGAAGACA AATTTAACCC GTCATTTATC ACCACCATTG GTATTGATTT CAAAATAAAG 
ACTGTCGATA TCAACGGTAA GAAGGTAAAG CTGCAACTTT GGGATACCGC TGGTCAAGAA 
CGTTTCCGGA CAATCACCAC AGCGTATTAT CGTGGTGCTA TGGGTATCAT TCTTGTATAT 
GATGTGACAG ACGAGAGAAC ATTTACTAAT ATCAAGCAAT GGTTTAAAAC CGTTAATGAG 
CATGCGAACG ATGAAGCACA GCTACTGTTG GTTGGTAACA AGAGCGATAT GGAGACGAGA 
GTGGTAACAG CTGATCAAGG TGAAGCCTTG GCTAAGGAGC TGGGTATACC ATTCATCGAG 
TCCAGTGCTA AGAACGATGA CAATGTCAAC GAGATTTTTT TCACCTTGGC GAAGTTAATC 
CAAGAAAAAA TCGACAGTAA CAAGCTTGTT GGCGTCGGTA ACGGTAAAGA GGGCAATATT 
AGCATCAATA GTGGGAGCGG AAACAGTTCT AAATCAAATT GCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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SEC4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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