CDC4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CDC4
Entrez Gene ID	850539
Full Name	SCF ubiquitin ligase complex subunit CDC4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179957.1	NP_116585.1	SCF ubiquitin ligase complex subunit CDC4

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AGL024W	NP_986642.1
Kluyveromyces lactis	KLLA0D07546g	XP_453399.1
Saccharomyces cerevisiae (baker's yeast)	CDC4	NP_116585.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our observations suggest that both APC(Cdh1) and SCF(Cdc4)-dependent proteolysis of Clb6 at the G1/S border are crucial for multiple cell cycle regulated events including proper expression of Clb2, the G1/S and G2/M cell cycle transitions and for proper completion of cell division at mitotic exit.
Title: The anaphase-promoting complex works together with the SCF complex for proteolysis of the S-phase cyclin Clb6 during the transition from G1 to S phase.

an intricate coordination between Hst3 synthesis, genome-wide H3K56 deacetylation by Hst3, and cell cycle-regulated degradation of Hst3 by cyclin-dependent kinases and SCF(Cdc4).
Title: Regulation of the histone deacetylase Hst3 by cyclin-dependent kinases and the ubiquitin ligase SCFCdc4.

Individually weak dispersed Sic1 phospho sites engage Cdc4 in a dynamic equilibrium. The multisite nature of this interaction confers cooperative dependence on kinase activity for Sic1 recognition
Title: Composite low affinity interactions dictate recognition of the cyclin-dependent kinase inhibitor Sic1 by the SCFCdc4 ubiquitin ligase.

we show that Cln3 is redundantly targeted by two F-box proteins with key cell cycle-regulatory roles, Cdc4 and Grr1
Title: F-box protein specificity for g1 cyclins is dictated by subcellular localization.

analysis of structure/function of a dynamic complex of the intrinsically disordered Sic1 with the Cdc4 subunit of an SCF ubiquitin ligase
Title: Structure/function implications in a dynamic complex of the intrinsically disordered Sic1 with the Cdc4 subunit of an SCF ubiquitin ligase.

The following CDC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00227	NM_001179957.1 Latest version!	Saccharomyces cerevisiae S288C SCF ubiquitin ligase complex subunit CDC4 (CDC4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDC4 ( NM_001179957.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116585.1 Saccharomyces cerevisiae S288C SCF ubiquitin ligase complex subunit CDC4 (CDC4), partial mRNA.

Price & Availability↑

CloneID	OSi00227
Clone ID Related Accession (Same CDS sequence)	NM_001179957.1
Accession Version	NM_001179957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	SCF ubiquitin ligase complex subunit CDC4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGGTCGT TTCCCTTAGC TGAGTTTCCA TTACGTGATA TCCCTGTTCC TTATAGCTAC 
CGTGTGTCTG GCGGTATAGC TTCCTCAGGT AGTGTTACTG CGCTTGTTAC TGCCGCTGGC 
ACTCATCGAA ACTCGTCCAC GGCTAAGACA GTTGAGACAG AAGACGGCGA AGAAGATATC 
GATGAGTATC AGAGGAAAAG AGCAGCTGGT TCTGGCGAAT CCACTCCTGA ACGCAGTGAT 
TTCAAAAGGG TAAAACATGA TAATCACAAA ACCCTCCATC CAGTTAACTT ACAGAACACC 
GGTGCAGCGT CTGTGGATAA TGACGGTCTG CACAATTTAA CAGATATATC CAACGATGCA 
GAAAAACTTT TGATGTCTGT GGATGATGGT TCTGCCGCAC CTTCTACATT GAGTGTAAAC 
ATGGGAGTGG CATCTCATAA TGTTGCTGCT CCCACTACCG TCAATGCGGC AACAATAACT 
GGCAGTGATG TTAGTAACAA TGTTAATAGT GCTACTATTA ACAATCCTAT GGAGGAAGGA 
GCGCTGCCGT TATCACCCAC TGCTTCCTCT CCAGGTACCA CAACTCCTTT AGCTAAAACT 
ACGAAAACTA TCAACAACAA TAATAATATC GCCGATTTGA TAGAATCCAA AGATTCTATA 
ATCTCCCCTG AATACCTTTC TGATGAGATT TTCAGCGCAA TAAACAATAA TCTCCCTCAC 
GCATACTTCA AAAATTTATT ATTTAGATTA GTTGCCAACA TGGATAGGAG TGAACTATCC 
GACTTGGGGA CTTTAATCAA GGATAATTTA AAGAGGGACC TAATAACGTC TTTGCCTTTT 
GAAATAAGTT TGAAAATTTT CAATTATTTG CAATTCGAGG ATATTATAAA TTCCCTTGGG 
GTCTCCCAAA ATTGGAACAA AATAATTAGA AAATCTACAT CGTTGTGGAA AAAACTTCTG 
ATATCGGAAA ATTTTGTGAG CCCAAAGGGT TTTAATTCTC TCAATCTCAA ACTCTCCCAA 
AAATACCCAA AACTCTCACA ACAAGATCGC CTTAGATTAT CTTTTCTGGA GAATATATTC 
ATTTTAAAAA ATTGGTACAA TCCCAAGTTT GTACCACAAA GGACCACGTT AAGAGGCCAT 
ATGACGAGTG TTATTACGTG CTTGCAATTT GAAGATAATT ATGTCATTAC GGGGGCTGAT 
GACAAAATGA TCAGAGTTTA TGATTCGATA AACAAGAAAT TTCTTCTACA ACTATCAGGT 
CATGATGGTG GGGTTTGGGC ATTGAAGTAT GCCCATGGCG GTATTTTAGT CAGCGGTTCT 
ACAGACAGAA CGGTGCGAGT TTGGGATATT AAGAAAGGTT GTTGTACCCA TGTGTTTAAA 
GGTCATAACT CTACGGTGAG GTGCCTAGAT ATAGTAGAAT ATAAAAATAT CAAGTACATT 
GTTACTGGTT CGAGAGATAA CACTTTGCAC GTTTGGAAAT TGCCCAAGGA GTCCTCCGTT 
CCTGATCATG GGGAAGAACA TGATTATCCA TTAGTCTTTC ATACCCCTGA GGAGAACCCA 
TATTTTGTTG GTGTTTTAAG AGGACATATG GCATCTGTAA GAACTGTCTC AGGCCACGGT 
AATATTGTCG TTAGTGGCTC CTATGATAAT ACACTGATTG TGTGGGATGT TGCGCAAATG 
AAATGTTTGT ATATTTTAAG TGGACATACG GATCGTATTT ATTCGACAAT CTACGATCAT 
GAAAGAAAAA GGTGCATCTC TGCCAGTATG GATACCACTA TTAGAATTTG GGATTTGGAA 
AATATATGGA ATAATGGAGA ATGTTCCTAC GCAACAAATT CAGCATCGCC ATGCGCCAAA 
ATACTTGGTG CTATGTACAC TTTGCAGGGT CATACAGCTT TGGTCGGTTT ATTAAGATTA 
TCCGACAAAT TTTTGGTCAG TGCCGCTGCA GACGGTTCAA TAAGGGGTTG GGACGCAAAC 
GACTACTCTA GAAAATTTTC CTACCATCAT ACCAATTTGA GTGCAATTAC CACATTTTAT 
GTATCGGATA ATATTTTGGT GAGTGGATCG GAAAATCAGT TCAACATCTA TAATCTACGG 
AGTGGGAAAT TGGTCCACGC AAATATTCTA AAAGATGCTG ATCAGATTTG GTCGGTTAAT 
TTTAAGGGCA AAACACTTGT TGCAGCAGTT GAAAAAGATG GACAGAGCTT TTTAGAAATT 
CTGGATTTCA GCAAAGCTTC AAAAATTAAC TACGTTAGCA ATCCCGTAAA CTCCTCGTCG 
TCGTCTTTGG AATCCATTTC TACTTCTTTG GGTCTAACGA GGACAACTAT AATACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116585.1
CDS	1..2340
Translation	MGSFPLAEFPLRDIPVPYSYRVSGGIASSGSVTALVTAAGTHRNSSTAKTVETEDGEEDIDEYQRKRAAGSGESTPERSDFKRVKHDNHKTLHPVNLQNTGAASVDNDGLHNLTDISNDAEKLLMSVDDGSAAPSTLSVNMGVASHNVAAPTTVNAATITGSDVSNNVNSATINNPMEEGALPLSPTASSPGTTTPLAKTTKTINNNNNIADLIESKDSIISPEYLSDEIFSAINNNLPHAYFKNLLFRLVANMDRSELSDLGTLIKDNLKRDLITSLPFEISLKIFNYLQFEDIINSLGVSQNWNKIIRKSTSLWKKLLISENFVSPKGFNSLNLKLSQKYPKLSQQDRLRLSFLENIFILKNWYNPKFVPQRTTLRGHMTSVITCLQFEDNYVITGADDKMIRVYDSINKKFLLQLSGHDGGVWALKYAHGGILVSGSTDRTVRVWDIKKGCCTHVFKGHNSTVRCLDIVEYKNIKYIVTGSRDNTLHVWKLPKESSVPDHGEEHDYPLVFHTPEENPYFVGVLRGHMASVRTVSGHGNIVVSGSYDNTLIVWDVAQMKCLYILSGHTDRIYSTIYDHERKRCISASMDTTIRIWDLENIWNNGECSYATNSASPCAKILGAMYTLQGHTALVGLLRLSDKFLVSAAADGSIRGWDANDYSRKFSYHHTNLSAITTFYVSDNILVSGSENQFNIYNLRSGKLVHANILKDADQIWSVNFKGKTLVAAVEKDGQSFLEILDFSKASKINYVSNPVNSSSSSLESISTSLGLTRTTIIP

Target ORF information:

RefSeq Version	NM_001179957.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C SCF ubiquitin ligase complex subunit CDC4 (CDC4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179957.1

ORF Insert Sequence:

ATGGGGTCGT TTCCCTTAGC TGAGTTTCCA TTACGTGATA TCCCTGTTCC TTATAGCTAC 
CGTGTGTCTG GCGGTATAGC TTCCTCAGGT AGTGTTACTG CGCTTGTTAC TGCCGCTGGC 
ACTCATCGAA ACTCGTCCAC GGCTAAGACA GTTGAGACAG AAGACGGCGA AGAAGATATC 
GATGAGTATC AGAGGAAAAG AGCAGCTGGT TCTGGCGAAT CCACTCCTGA ACGCAGTGAT 
TTCAAAAGGG TAAAACATGA TAATCACAAA ACCCTCCATC CAGTTAACTT ACAGAACACC 
GGTGCAGCGT CTGTGGATAA TGACGGTCTG CACAATTTAA CAGATATATC CAACGATGCA 
GAAAAACTTT TGATGTCTGT GGATGATGGT TCTGCCGCAC CTTCTACATT GAGTGTAAAC 
ATGGGAGTGG CATCTCATAA TGTTGCTGCT CCCACTACCG TCAATGCGGC AACAATAACT 
GGCAGTGATG TTAGTAACAA TGTTAATAGT GCTACTATTA ACAATCCTAT GGAGGAAGGA 
GCGCTGCCGT TATCACCCAC TGCTTCCTCT CCAGGTACCA CAACTCCTTT AGCTAAAACT 
ACGAAAACTA TCAACAACAA TAATAATATC GCCGATTTGA TAGAATCCAA AGATTCTATA 
ATCTCCCCTG AATACCTTTC TGATGAGATT TTCAGCGCAA TAAACAATAA TCTCCCTCAC 
GCATACTTCA AAAATTTATT ATTTAGATTA GTTGCCAACA TGGATAGGAG TGAACTATCC 
GACTTGGGGA CTTTAATCAA GGATAATTTA AAGAGGGACC TAATAACGTC TTTGCCTTTT 
GAAATAAGTT TGAAAATTTT CAATTATTTG CAATTCGAGG ATATTATAAA TTCCCTTGGG 
GTCTCCCAAA ATTGGAACAA AATAATTAGA AAATCTACAT CGTTGTGGAA AAAACTTCTG 
ATATCGGAAA ATTTTGTGAG CCCAAAGGGT TTTAATTCTC TCAATCTCAA ACTCTCCCAA 
AAATACCCAA AACTCTCACA ACAAGATCGC CTTAGATTAT CTTTTCTGGA GAATATATTC 
ATTTTAAAAA ATTGGTACAA TCCCAAGTTT GTACCACAAA GGACCACGTT AAGAGGCCAT 
ATGACGAGTG TTATTACGTG CTTGCAATTT GAAGATAATT ATGTCATTAC GGGGGCTGAT 
GACAAAATGA TCAGAGTTTA TGATTCGATA AACAAGAAAT TTCTTCTACA ACTATCAGGT 
CATGATGGTG GGGTTTGGGC ATTGAAGTAT GCCCATGGCG GTATTTTAGT CAGCGGTTCT 
ACAGACAGAA CGGTGCGAGT TTGGGATATT AAGAAAGGTT GTTGTACCCA TGTGTTTAAA 
GGTCATAACT CTACGGTGAG GTGCCTAGAT ATAGTAGAAT ATAAAAATAT CAAGTACATT 
GTTACTGGTT CGAGAGATAA CACTTTGCAC GTTTGGAAAT TGCCCAAGGA GTCCTCCGTT 
CCTGATCATG GGGAAGAACA TGATTATCCA TTAGTCTTTC ATACCCCTGA GGAGAACCCA 
TATTTTGTTG GTGTTTTAAG AGGACATATG GCATCTGTAA GAACTGTCTC AGGCCACGGT 
AATATTGTCG TTAGTGGCTC CTATGATAAT ACACTGATTG TGTGGGATGT TGCGCAAATG 
AAATGTTTGT ATATTTTAAG TGGACATACG GATCGTATTT ATTCGACAAT CTACGATCAT 
GAAAGAAAAA GGTGCATCTC TGCCAGTATG GATACCACTA TTAGAATTTG GGATTTGGAA 
AATATATGGA ATAATGGAGA ATGTTCCTAC GCAACAAATT CAGCATCGCC ATGCGCCAAA 
ATACTTGGTG CTATGTACAC TTTGCAGGGT CATACAGCTT TGGTCGGTTT ATTAAGATTA 
TCCGACAAAT TTTTGGTCAG TGCCGCTGCA GACGGTTCAA TAAGGGGTTG GGACGCAAAC 
GACTACTCTA GAAAATTTTC CTACCATCAT ACCAATTTGA GTGCAATTAC CACATTTTAT 
GTATCGGATA ATATTTTGGT GAGTGGATCG GAAAATCAGT TCAACATCTA TAATCTACGG 
AGTGGGAAAT TGGTCCACGC AAATATTCTA AAAGATGCTG ATCAGATTTG GTCGGTTAAT 
TTTAAGGGCA AAACACTTGT TGCAGCAGTT GAAAAAGATG GACAGAGCTT TTTAGAAATT 
CTGGATTTCA GCAAAGCTTC AAAAATTAAC TACGTTAGCA ATCCCGTAAA CTCCTCGTCG 
TCGTCTTTGG AATCCATTTC TACTTCTTTG GGTCTAACGA GGACAACTAT AATACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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CDC4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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