Gene Symbol | NFS1 |
Entrez Gene ID | 850343 |
Full Name | Nfs1p |
Synonyms | SPL1 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
ORF » Species Summary » Saccharomyces cerevisiae S288C » NFS1 cDNA ORF clone
Gene Symbol | NFS1 |
Entrez Gene ID | 850343 |
Full Name | Nfs1p |
Synonyms | SPL1 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
mRNA | Protein | Name |
---|---|---|
NM_001178664.1 | NP_009912.2 | cysteine desulfurase |
Homo sapiens (human) | NFS1 | NP_066923.3 |
Drosophila melanogaster (fruit fly) | CG12264 | NP_609533.1 |
Kluyveromyces lactis | KLLA0D04928g | XP_453279.1 |
Oryza sativa (rice) | Os09g0338400 | NP_001062914.1 |
Xenopus tropicalis (tropical clawed frog) | nfs1 | XP_002932937.2 |
Pan troglodytes (chimpanzee) | NFS1 | XP_001165456.1 |
NFS1 | XP_001097983.1 | |
Mus musculus (house mouse) | Nfs1 | NP_035041.2 |
Rattus norvegicus (Norway rat) | Nfs1 | NP_445914.2 |
Rattus norvegicus (Norway rat) | LOC100911034 | XP_003749657.1 |
Gallus gallus (chicken) | NFS1 | NP_001026018.1 |
Saccharomyces cerevisiae (baker's yeast) | NFS1 | NP_009912.2 |
Eremothecium gossypii | AGOS_AAL013W | NP_982529.1 |
Arabidopsis thaliana (thale cress) | NFS1 | NP_201373.1 |
Bos taurus (cattle) | NFS1 | NP_001092471.1 |
Gallus gallus (chicken) | LOC100858210 | XP_003642486.1 |
Caenorhabditis elegans (roundworm) | B0205.6 | NP_492812.2 |
Schizosaccharomyces pombe (fission yeast) | nfs1 | NP_596002.2 |
Magnaporthe oryzae (rice blast fungus) | MGG_17371 | XP_003718229.1 |
Neurospora crassa | NCU04636 | XP_959179.1 |
Canis lupus familiaris (dog) | NFS1 | XP_534405.2 |
Anopheles gambiae (African malaria mosquito) | AgaP_AGAP009094 | XP_319845.3 |
Danio rerio (zebrafish) | nfs1 | NP_001038458.1 |
Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
The complete DNA sequence of yeast chromosome III.
Oliver SG, van der Aart QJ, Agostoni-Carbone ML, Aigle M, Alberghina L, Alexandraki D, Antoine G, Anwar R, Ballesta JP, Benit P
Nature357(6373)38-46(1992 May)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Isu is a substrate of the Lon-type protease and that it is protected from degradation by Nfs1. Nfs1 variants known to be defective in interaction with Isu were also defective in protecting Isu from degradation.
Title: Protection of scaffold protein Isu from degradation by the Lon protease Pim1 as a component of Fe-S cluster biogenesis regulation.
Mutual exclusivity of cluster assembly factor (Nfs1/Yfh1) and cluster transfer factor (Jac1/Ssq1) binding to Isu has functional consequences for the transition from the assembly process to the transfer process during biogenesis of FeS cluster proteins.
Title: Overlapping binding sites of the frataxin homologue assembly factor and the heat shock protein 70 transfer factor on the Isu iron-sulfur cluster scaffold protein.
The data presented here show that the Isu1 suppressor mimics the frataxin effects on Nfs1, explaining the bypassing activity.
Title: Frataxin directly stimulates mitochondrial cysteine desulfurase by exposing substrate-binding sites, and a mutant Fe-S cluster scaffold protein with frataxin-bypassing ability acts similarly.
Competition between Jac1 and Nfs1 for Isu binding plays an important role in transitioning the iron/Sulfur cluster biogenesis machinery.
Title: Binding of the chaperone Jac1 protein and cysteine desulfurase Nfs1 to the iron-sulfur cluster scaffold Isu protein is mutually exclusive.
results show that Nfs1 adopts a higher oligomeric state in the Isd11-bound form compared to that in the free form
Title: The effect of the adaptor protein Isd11 on the quaternary structure of the eukaryotic cysteine desulphurase Nfs1.
The following NFS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSi00066 | |
Clone ID Related Accession (Same CDS sequence) | NM_001178664.1 | |
Accession Version | NM_001178664.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-31 | |
Organism | Saccharomyces cerevisiae S288C | |
Product | cysteine desulfurase | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001135). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. |
1 | ATGTTGAAAT CAACTGCTAC AAGATCGATA ACAAGATTAT CTCAAGTTTA CAACGTTCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_009912.2 |
CDS | 1..1494 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001178664.1 |
1 | ATGTTGAAAT CAACTGCTAC AAGATCGATA ACAAGATTAT CTCAAGTTTA CAACGTTCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Life with 6000 genes. |
The complete DNA sequence of yeast chromosome III. |