wtf25 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following wtf25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wtf25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSj00135 XM_004001707.1
Latest version!
Schizosaccharomyces pombe 972h- wtf element (wtf25), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OSc99887 NM_001355948.1
Latest version!
Schizosaccharomyces pombe wtf element (wtf25), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSj00135
    Clone ID Related Accession (Same CDS sequence) XM_004001707.1
    Accession Version XM_004001707.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2012-12-10
    Organism Schizosaccharomyces pombe 972h-
    Product wtf element
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAGAATA ATTACACTTC TTTGAAAAGT CCTCTAGATG AAGAAGATGA ATTGAAGACA 
    GATCATGAAA TCGATTTGGA AAAAGGACCA CTCCCAGAAT ATGATTCGGA GGAAGAGGGC
    GCGTTACCAC CATATTCAGA TCATGCACTT GTAAATAATC CTCTTAACAC ACATAGAGAA
    AACCATTCTT ATGGGACGAC AGATAATTCT TCCCCTTTGT TGATAATACT GCTAATATCA
    TTCACTTCAA TCATTCTATT CAATGCTCCA GCATTTTGTT ATCTAAAGTA TAAGGACGCT
    TTTTTTAAAA ATTATGGTGC AGCGGAATGG ACATTATTTG GATTTTGGTG TCTTGTTTGC
    ACTTTGGCTT TAATATTCTT GACTTACTTC TATGAAACTT GGTCCAAGGC TTGCGGAAAA
    GGAATCAAGC ACTTTTTAAA AAATTGGAGA AACATGATTT TTGCTTTTTG TAAAAGTTCT
    TTATTTTGCT TGGTTTTATT AAAAGCTGAA AACAAATTGA GTTCACATTT GGGTGATCAA
    CGATGGGGGT GGAAATGCTC GGCATCCGCT TTCACTTTCA TGGCTGTATC AAGCATTCTC
    ATATTCATTG CTGAAACAGT GGAACCGGGA TCTTGCTCTA CAGATTTGGT GAAAAGAACA
    CTAGCATTTT ATGGTTATGA GATCAGACAG CACGTGAATG AAGACGCCAC TATTCTGTTG
    CGTGAAATGA ACCCTGAAAG TGAAGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004001756.1
    CDS211..960
    Translation

    Target ORF information:

    RefSeq Version XM_004001707.1
    Organism Schizosaccharomyces pombe 972h-
    Definition Schizosaccharomyces pombe 972h- wtf element (wtf25), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004001707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAGAATA ATTACACTTC TTTGAAAAGT CCTCTAGATG AAGAAGATGA ATTGAAGACA 
    GATCATGAAA TCGATTTGGA AAAAGGACCA CTCCCAGAAT ATGATTCGGA GGAAGAGGGC
    GCGTTACCAC CATATTCAGA TCATGCACTT GTAAATAATC CTCTTAACAC ACATAGAGAA
    AACCATTCTT ATGGGACGAC AGATAATTCT TCCCCTTTGT TGATAATACT GCTAATATCA
    TTCACTTCAA TCATTCTATT CAATGCTCCA GCATTTTGTT ATCTAAAGTA TAAGGACGCT
    TTTTTTAAAA ATTATGGTGC AGCGGAATGG ACATTATTTG GATTTTGGTG TCTTGTTTGC
    ACTTTGGCTT TAATATTCTT GACTTACTTC TATGAAACTT GGTCCAAGGC TTGCGGAAAA
    GGAATCAAGC ACTTTTTAAA AAATTGGAGA AACATGATTT TTGCTTTTTG TAAAAGTTCT
    TTATTTTGCT TGGTTTTATT AAAAGCTGAA AACAAATTGA GTTCACATTT GGGTGATCAA
    CGATGGGGGT GGAAATGCTC GGCATCCGCT TTCACTTTCA TGGCTGTATC AAGCATTCTC
    ATATTCATTG CTGAAACAGT GGAACCGGGA TCTTGCTCTA CAGATTTGGT GAAAAGAACA
    CTAGCATTTT ATGGTTATGA GATCAGACAG CACGTGAATG AAGACGCCAC TATTCTGTTG
    CGTGAAATGA ACCCTGAAAG TGAAGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSc99887
    Clone ID Related Accession (Same CDS sequence) NM_001355948.1
    Accession Version NM_001355948.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product wtf element
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAGAATA ATTACACTTC TTTGAAAAGT CCTCTAGATG AAGAAGATGA ATTGAAGACA 
    GATCATGAAA TCGATTTGGA AAAAGGACCA CTCCCAGAAT ATGATTCGGA GGAAGAGGGC
    GCGTTACCAC CATATTCAGA TCATGCACTT GTAAATAATC CTCTTAACAC ACATAGAGAA
    AACCATTCTT ATGGGACGAC AGATAATTCT TCCCCTTTGT TGATAATACT GCTAATATCA
    TTCACTTCAA TCATTCTATT CAATGCTCCA GCATTTTGTT ATCTAAAGTA TAAGGACGCT
    TTTTTTAAAA ATTATGGTGC AGCGGAATGG ACATTATTTG GATTTTGGTG TCTTGTTTGC
    ACTTTGGCTT TAATATTCTT GACTTACTTC TATGAAACTT GGTCCAAGGC TTGCGGAAAA
    GGAATCAAGC ACTTTTTAAA AAATTGGAGA AACATGATTT TTGCTTTTTG TAAAAGTTCT
    TTATTTTGCT TGGTTTTATT AAAAGCTGAA AACAAATTGA GTTCACATTT GGGTGATCAA
    CGATGGGGGT GGAAATGCTC GGCATCCGCT TTCACTTTCA TGGCTGTATC AAGCATTCTC
    ATATTCATTG CTGAAACAGT GGAACCGGGA TCTTGCTCTA CAGATTTGGT GAAAAGAACA
    CTAGCATTTT ATGGTTATGA GATCAGACAG CACGTGAATG AAGACGCCAC TATTCTGTTG
    CGTGAAATGA ACCCTGAAAG TGAAGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001343062.1
    CDS211..960
    Translation

    Target ORF information:

    RefSeq Version NM_001355948.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe wtf element (wtf25), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355948.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAGAATA ATTACACTTC TTTGAAAAGT CCTCTAGATG AAGAAGATGA ATTGAAGACA 
    GATCATGAAA TCGATTTGGA AAAAGGACCA CTCCCAGAAT ATGATTCGGA GGAAGAGGGC
    GCGTTACCAC CATATTCAGA TCATGCACTT GTAAATAATC CTCTTAACAC ACATAGAGAA
    AACCATTCTT ATGGGACGAC AGATAATTCT TCCCCTTTGT TGATAATACT GCTAATATCA
    TTCACTTCAA TCATTCTATT CAATGCTCCA GCATTTTGTT ATCTAAAGTA TAAGGACGCT
    TTTTTTAAAA ATTATGGTGC AGCGGAATGG ACATTATTTG GATTTTGGTG TCTTGTTTGC
    ACTTTGGCTT TAATATTCTT GACTTACTTC TATGAAACTT GGTCCAAGGC TTGCGGAAAA
    GGAATCAAGC ACTTTTTAAA AAATTGGAGA AACATGATTT TTGCTTTTTG TAAAAGTTCT
    TTATTTTGCT TGGTTTTATT AAAAGCTGAA AACAAATTGA GTTCACATTT GGGTGATCAA
    CGATGGGGGT GGAAATGCTC GGCATCCGCT TTCACTTTCA TGGCTGTATC AAGCATTCTC
    ATATTCATTG CTGAAACAGT GGAACCGGGA TCTTGCTCTA CAGATTTGGT GAAAAGAACA
    CTAGCATTTT ATGGTTATGA GATCAGACAG CACGTGAATG AAGACGCCAC TATTCTGTTG
    CGTGAAATGA ACCCTGAAAG TGAAGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L