fas2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	fas2
Entrez Gene ID	2543618
Full Name	fatty acid synthase alpha subunit Lsd1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019252.2	NP_593823.1	fatty acid synthase alpha subunit Lsd1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	FAS2	NP_015093.1
Kluyveromyces lactis	KLLA0C15983g	XP_452914.1
Neurospora crassa	NCU07308	XP_962466.1
Eremothecium gossypii	AGOS_AFL138W	NP_985412.2
Schizosaccharomyces pombe (fission yeast)	fas2	NP_593823.1
Magnaporthe oryzae (rice blast fungus)	MGG_12154	XP_003719713.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The importance of both enzymatic and nonenzymatic roles of spLsd1 in regulating heterochromatin propagation and euchromatic transcription and also suggest that misregulation of spLsd1/2 is likely to impact the epigenetic state of the cell [spLsd1].
Title: S. pombe LSD1 homologs regulate heterochromatin propagation and euchromatic gene transcription.

The following fas2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fas2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102418	NM_001019252.2 Latest version!	Schizosaccharomyces pombe fatty acid synthase alpha subunit Lsd1 (fas2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fas2 ( NM_001019252.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593823.1 Schizosaccharomyces pombe fatty acid synthase alpha subunit Lsd1 (fas2), mRNA.

Price & Availability↑

CloneID	OSc102418
Clone ID Related Accession (Same CDS sequence)	NM_001019252.2
Accession Version	NM_001019252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	fatty acid synthase alpha subunit Lsd1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019252.1.

ATGAGACCAG AAGTTGAGCA GGAGCTTGCT CATACTTTAT TATTGGAGTT GCTTGCATAC 
CAGTTTGCAT CTCCTGTCCG TTGGATTGAG ACGCAAGATG TAATTCTTTC TCCTCCAGTA 
TCGGCTGAAC GTATCGTCGA AATTGGACCT AGTCCTACCT TAGCTGGTAT GGCTAAGCGT 
ACCTTGAAAT TGAAATATGA GAACATGGAT GCCGCTTTAA GTATTAATCG TGAAGTTCTT 
TGCTACTCTA AAGATGCTCG TGAAATCTAT TACAACTTTG AGGACGAGGT TGCTGATGAA 
CCTGCCGAAG CCCCAGCTTC AACCAGCTCC ACTCCAAAGG TTGAAACTGC TGCTGCTGCC 
GCTCCCGCTG CCACGCCAGC CCCTGCCCCA GCACAAACAT CAGCCCCAGC TGCTGCTTTA 
CCTGACGAGC CTCCCAAAGC TCTTGAGGTA CTTCATACTC TTGTTGCCCA AAAGTTGAAG 
AAAAGCATCG AGGAAGTCTC CCCTCAAAAA TCTATCAAAG ATTTGGTTGG CGGTAAGTCC 
ACTTTGCAAA ACGAAATTCT TGGTGATTTA CAGAAGGAGT TCGGTGCCAC TCCCGAGAAG 
CCAGAGGAGG TTCCATTGGA TGAGCTTGGA GCTATCATGC AGTCAAGCTT TAACGGATCT 
CTTGGTAAAC AATCGTCTTC TCTTATCTCA CGAATGATTT CCTCAAAAAT GCCTGGTGGT 
TTCAATAATT CTGCTGTTCG TGGTTATTTA GGAAACCGTT ATGGTTTGGG TCCTGGTCGT 
TTGGAGTCTG TGCTTTTGTT AGCGCTTACC ATGGAACCTG CATCACGTTT GGGCTCGGAA 
GCTGATGCTA AAGCTTGGCT TGATAGTGTA GCTCAAAAAT ATGCTGCTCG TAATGGTGTT 
ACATTATCTT CTCCTACTGC TGAAGGCGGT TCTTCGTCCG GTTCTGCAGC TGTTATCGAT 
GAAGAAACCT TTAAGAAACT CACCAAGAAT AATACCATGC TTGTTACTCA GCAATTAGAA 
CTATTTGCTC GATACCTCAA TAAAGACCTT CGTGCTGGCC AAAAGGCTCA AGTTGCTGAA 
AAGGTTATTT CCGATACCTT ACGCGCTCAA TTAGATTTAT GGAACGAAGA ACATGGTGAA 
TTTTATGCAT CAGGAATTGC TCCTATTTTT TCGCCTTTAA AAGCTCGCGT TTACGACTCC 
GACTGGAATT GGGCTCGTCA AGATGCTCTT AAGATGTTTT TTGACATTAT CTTTGGTCGT 
CTTAAGCATG TTGATACTGA AATAGTCGCT CGTTGTATTT CTGTTATGAA TAGATCCAAC 
CCTACTTTAC TTGAATTTAT GCAATATCAT ATTGATCATT GTCCCGCCGA AAAGGGTGAA 
ACATATCAAC TTGCTAAAAC CTTGGGCCAA CAGCTAATTG ATAATTGCAA ATCCGTGATA 
GATGCTCCTC CAGTTTTCAA AAATGTGAAT CATCCAACTG CTCCTTCTAC GACGATTGAC 
GAACGTGGTA ATTTGAATTA TGAAGAAATC CCTAGACCAG GTGTTCGCAA ATTAACTCAT 
TACGTTACTG AGATGGCCAA AGGTGGTAAA TTACCAACGG AGTCCAAAAA CAAAGCTAAG 
GTACAAAACG ATTTGGCTCG AATTTATCGC ATTATTAAGT CTCAAAACAA AATGTCTCGT 
TCGTCTAAGT TGCAGATTAA ACAGTTGTAC GGTCAGGTTT TACATGCCCT TTCCCTTCCA 
TTGCCTTCTT CCAACGATGA ACAAACGCCT GTTAAAGAAA CCATTCCTTT CCTTCATATT 
AGGAAGAAGT CCGTTGATGG TAATTGGGAA TTCAACAAGT CATTGACTGG CACTTATTTA 
GATGTTTTAG AATCGGGTGC TAAGAATGGT ATAACATACC AAGACAAATA TGCTCTAGTG 
ACTGGTGCAG GTGCAGGCTC CATTGGTGCT CAGATTGTTG AAGGTCTCCT TGCTGGTGGT 
GCTAAAGTTG TAGTTACTAC ATCCCGGTTT TCGCGCAAGG TTACTGAATT TTATCAATCC 
CTTTACACCC GCCATGGAAG CCGTGGTTCA TGTCTGATCG TGGTTCCATT TAACCAAGGA 
TCTAAGACAG ACGTAGAAGC TCTTATTGAT TATATTTATG ACGAAAAGAA GGGTCTTGGA 
TGGAACTTGG ACTACATTGT TCCTTTCGCT GCCATTCCAG AAAATGGTCG TGAAATTGAT 
GGCATTGATT CTCGTTCCGA GTTTGCTCAC CGTATTATGT TGACAAACAT TTTGAGACTG 
CTTGGCGCCG TCAAAAGTCA AAAGGCCTCT CGTGGTATGG ATACCCGACC CGCTCAAGTT 
ATTTTGCCTC TTTCTCCCAA TCACGGTACC TTTGGAAACG ATGGTTTATA CTCGGAATCT 
AAGTTAGGTT TAGAAACTTT GTTTAACCGT TGGTACTCCG AGTCATGGGC TAATTACCTA 
ACCATTTGTG GGGCTGTCAT TGGTTGGACT CGTGGTACAG GCTTAATGGC ACCTAATAAT 
ATTGTTTCTC AGGGAATCGA AAAATATGGT GTTCGTACTT TTTCGCAGAG TGAGATGGCT 
TTTAACATTT TGGGTTTGAT GTCCCAGAAA GTCGTCGACT TGTGTCAATC TGAACCAATT 
TATGCCAACC TTAACGGTGG TCTTGAGCTT TTACCTGATC TCAAGGACCT TTCCACTCGT 
TTGCGTACCG AATTGTTAGA AACTGCCGAA ATCCGCCGCG CTGTTGCCGC AGAGACTGCC 
TTTGATCATA GCATTACCAA CGGACCTGAC TCTGAAGCAG TTTTCCAGAA AACTGCCATT 
CAGCCTAGGG CCAATCTTAA ATTTAATTTC CCCAAATTGA AACCTTATGA AGCCCTTTCT 
CATTTATCTG ATCTTCGTGG AATGGTTGAT TTAGAAAAAG TTCCTGTTGT TACTGGTTTT 
TCCGAAGTAG GTCCATGGGG TAACTCTCGT ACTAGATGGG ATATGGAGTG TTATGGTGAG 
TTTTCACTAG AAGGATGTGT CGAAATTGCT TGGATTATGG GATTAATTAA AAACTTCAAT 
GGCAAGGGCA AAGACGGCAA GCCCTATTCA GGTTGGGTTG ATACAAAGAC CGGTGAACCT 
GTGGACGACA AAGACGTTAA AGCTAAGTAT GAGAAGTATA TACTGGAGCA TTGCGGTATC 
CGTATTATTG AAGCTGAACT CTTCCATGGA TATAATCCTG AAAAGAAAGA GCTTTTGCAA 
GAAGTTGTTA TTGATCATGA CTTAGAGCCT TTTGAAGCAT CCAAAGAGGC TGCTCATGAG 
TTCAAGCTTC GTCATGGTGA TCAAGTTGAA ATTTTTGAAA TTCCTGATTC TACCGAATGG 
TCCGTACGCT TCAAGCGCGG TACAAGTATG CTAATTCCTA AGGCTTTGCG CTTTGATCGA 
TTTGTTGCTG GCCAGATTCC ACTTGGTTGG GATCCCAAAC GTTATGGCAT TCCTGACGAT 
ATTATTTCTC AAGTTGACCC TACAACTTTG TACGTTTTAG TGTCTACTGT AGAAGCTCTG 
GTTGCATCAG GTATTACAGA TCCTTATGAA TGCTATAAGT ATATTCACGT ATCTGAACTT 
GGTAATACAG TTGGTTCTGG TATTGGTGGT ATGTCTGCTC TTCGTGGAAT GTACAAGGAC 
CGCTGGACTG ATAAACCTGT TCAAAAAGAT ATTTTACAAG AATCATTCAT TAACACTGCC 
AATGCTTGGA TTAACATGCT TTTGCTCTCT GCCTCTGGTC CTATTAAGAC TCCTGTTGGT 
GCTTGCGCTA CCGCTGTCGA ATCTGTTGAT GCAGCTGTCG ACTTGATCAC TTCTGGTAAG 
GCCAGGATAT GTATTAGCGG TGGTTATGAC GACTTTTCAG AAGAAGGTTC ATACGAGTTT 
GCGAACATGG GTGCTACATC AAATGCTGCT AAGGAAACAG AAAGGGGACG TACTCCTCAA 
GAAATGTCTC GTCCTGCTAC TTCTACTCGT GATGGATTTA TGGAGTCTCA AGGTGCTGGT 
GTACAGATTA TCATGCAAGC AAAGCTTGCT ATTGAGATGG GTGTCCCTAT ACATGGTATT 
GTTGGTTATG TTTCCACAGC TATGGATAAA CAAGGTCGTT CGGTTCCTGC CCCTGGGCAA 
GGTATTTTGA CTGGTGCTCG TGAAATCGCG ACTAAGACAC CCCTTCCCAT AGTTGACCTT 
AAATTCCGTT CTCGTCAACT CCAACGCCGC CGTTCTCAAA TTGGTGAATG GGCCGAACGC 
GAGTATCTTT ATTTAGAAGA AGAACTTGAT GCGATGAAGG TTCAAAATCC TGACTTGGAT 
TTAGAGGCTT ACCGTATAGA GCGTATCAAC GTTATTAAGG AGGAGGTTGT TCGACAAGAA 
AAGGAGGCGC TCAATACTTT TGGAAATGAA TTTTGGAAAC GTGATCCTAC TATTGCTCCT 
ATCCGTGGTG CATTAGCTGT TTGGGGTCTT ACTATTGACG ATTTGGGCGT TGCATCATTC 
CATGGTACCT CTACCAAAGC CAATGAGAAG AATGAATGCG ATGTCATTGA CAGTCAGTTA 
ACACATCTCG GACGCTCTAA GGGTAACGCT GTGTACGGTG TTTTCCAGAA ATATCTCACT 
GGACATAGCA AGGGTGGTGC TGGAGCTTGG ATGCTCAACG GAGCTCTCCA AATTCTTCGC 
TCTGGGTTTG TTCCGGGTAA TCGTAACGCC GATAACATTG ATGAGTATCT AGCACGATTC 
GACCGGGTTA TGTTCCCTAG TGAAGGTATA CAAACTGATG GCATAAAGGC AGCATCTGTT 
ACTGCATTTG GTTTTGGACA AGTTGGTGGA CAAGTTATAG TTATCCATCC TGATTACATT 
TACGGTGTGA TTGATGAGGC TACTTATAAT GCTTACAAAG CTAAAACTGC TGCTCGTTAT 
AAGGCATCTT ATCGTTACAC CCACGATGCG CTGGTTTACA ACAATTTGGT CCGCGCCAAG 
GATTCTCCTC CTTACACCAA AGAACAAGAG AAAGCCGTTT ATCTCAATCC TTTGGCACGC 
GCTTCGAAGA GCAAAGCTGG CACTTGGACT TTCCCTGCCA CACTGCCTGC TGAATCCGAC 
ATTTCTAAAA CCAACGAAAC TACACGTACT CTACAAAGCC TAACAACCTC ATTGACCAAC 
TCCAATGAAA ATGTTGGCGT GGATGTTGAA CTTGTATCAG CGATTAGCAT TGATAATGAG 
ACCTTTATAG AAAGGAATTT TACTGATACC GAGCGAAAGT ACTGTTTTGC AGCTCCTAAT 
CCCCAAGCTA GCTTTGCCGG ACGTTGGTCA GCCAAAGAGG CTGTCTTTAA GTCTTTGGGT 
ATTTCCGGTA AAGGCGCTGC AGCTCCATTG AAGGATATCG AAATTATTTC TTCAGAGTCT 
GGTGCTCCTG AAGTAGTTTT GCACGGAGAG GCTGCGAAGG CTGCAACGAC CGCCGGTGTG 
AAGAGTGTTT CCGTCAGTAT TTCCCACGAT GATAATCAAA GTGTCAGTGT TGCTTTGGCT 
CACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593823.1
CDS	58..5586
Translation	MRPEVEQELAHTLLLELLAYQFASPVRWIETQDVILSPPVSAERIVEIGPSPTLAGMAKRTLKLKYENMDAALSINREVLCYSKDAREIYYNFEDEVADEPAEAPASTSSTPKVETAAAAAPAATPAPAPAQTSAPAAALPDEPPKALEVLHTLVAQKLKKSIEEVSPQKSIKDLVGGKSTLQNEILGDLQKEFGATPEKPEEVPLDELGAIMQSSFNGSLGKQSSSLISRMISSKMPGGFNNSAVRGYLGNRYGLGPGRLESVLLLALTMEPASRLGSEADAKAWLDSVAQKYAARNGVTLSSPTAEGGSSSGSAAVIDEETFKKLTKNNTMLVTQQLELFARYLNKDLRAGQKAQVAEKVISDTLRAQLDLWNEEHGEFYASGIAPIFSPLKARVYDSDWNWARQDALKMFFDIIFGRLKHVDTEIVARCISVMNRSNPTLLEFMQYHIDHCPAEKGETYQLAKTLGQQLIDNCKSVIDAPPVFKNVNHPTAPSTTIDERGNLNYEEIPRPGVRKLTHYVTEMAKGGKLPTESKNKAKVQNDLARIYRIIKSQNKMSRSSKLQIKQLYGQVLHALSLPLPSSNDEQTPVKETIPFLHIRKKSVDGNWEFNKSLTGTYLDVLESGAKNGITYQDKYALVTGAGAGSIGAQIVEGLLAGGAKVVVTTSRFSRKVTEFYQSLYTRHGSRGSCLIVVPFNQGSKTDVEALIDYIYDEKKGLGWNLDYIVPFAAIPENGREIDGIDSRSEFAHRIMLTNILRLLGAVKSQKASRGMDTRPAQVILPLSPNHGTFGNDGLYSESKLGLETLFNRWYSESWANYLTICGAVIGWTRGTGLMAPNNIVSQGIEKYGVRTFSQSEMAFNILGLMSQKVVDLCQSEPIYANLNGGLELLPDLKDLSTRLRTELLETAEIRRAVAAETAFDHSITNGPDSEAVFQKTAIQPRANLKFNFPKLKPYEALSHLSDLRGMVDLEKVPVVTGFSEVGPWGNSRTRWDMECYGEFSLEGCVEIAWIMGLIKNFNGKGKDGKPYSGWVDTKTGEPVDDKDVKAKYEKYILEHCGIRIIEAELFHGYNPEKKELLQEVVIDHDLEPFEASKEAAHEFKLRHGDQVEIFEIPDSTEWSVRFKRGTSMLIPKALRFDRFVAGQIPLGWDPKRYGIPDDIISQVDPTTLYVLVSTVEALVASGITDPYECYKYIHVSELGNTVGSGIGGMSALRGMYKDRWTDKPVQKDILQESFINTANAWINMLLLSASGPIKTPVGACATAVESVDAAVDLITSGKARICISGGYDDFSEEGSYEFANMGATSNAAKETERGRTPQEMSRPATSTRDGFMESQGAGVQIIMQAKLAIEMGVPIHGIVGYVSTAMDKQGRSVPAPGQGILTGAREIATKTPLPIVDLKFRSRQLQRRRSQIGEWAEREYLYLEEELDAMKVQNPDLDLEAYRIERINVIKEEVVRQEKEALNTFGNEFWKRDPTIAPIRGALAVWGLTIDDLGVASFHGTSTKANEKNECDVIDSQLTHLGRSKGNAVYGVFQKYLTGHSKGGAGAWMLNGALQILRSGFVPGNRNADNIDEYLARFDRVMFPSEGIQTDGIKAASVTAFGFGQVGGQVIVIHPDYIYGVIDEATYNAYKAKTAARYKASYRYTHDALVYNNLVRAKDSPPYTKEQEKAVYLNPLARASKSKAGTWTFPATLPAESDISKTNETTRTLQSLTTSLTNSNENVGVDVELVSAISIDNETFIERNFTDTERKYCFAAPNPQASFAGRWSAKEAVFKSLGISGKGAAAPLKDIEIISSESGAPEVVLHGEAAKAATTAGVKSVSVSISHDDNQSVSVALAHK

Target ORF information:

RefSeq Version	NM_001019252.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe fatty acid synthase alpha subunit Lsd1 (fas2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019252.2

ORF Insert Sequence:

ATGAGACCAG AAGTTGAGCA GGAGCTTGCT CATACTTTAT TATTGGAGTT GCTTGCATAC 
CAGTTTGCAT CTCCTGTCCG TTGGATTGAG ACGCAAGATG TAATTCTTTC TCCTCCAGTA 
TCGGCTGAAC GTATCGTCGA AATTGGACCT AGTCCTACCT TAGCTGGTAT GGCTAAGCGT 
ACCTTGAAAT TGAAATATGA GAACATGGAT GCCGCTTTAA GTATTAATCG TGAAGTTCTT 
TGCTACTCTA AAGATGCTCG TGAAATCTAT TACAACTTTG AGGACGAGGT TGCTGATGAA 
CCTGCCGAAG CCCCAGCTTC AACCAGCTCC ACTCCAAAGG TTGAAACTGC TGCTGCTGCC 
GCTCCCGCTG CCACGCCAGC CCCTGCCCCA GCACAAACAT CAGCCCCAGC TGCTGCTTTA 
CCTGACGAGC CTCCCAAAGC TCTTGAGGTA CTTCATACTC TTGTTGCCCA AAAGTTGAAG 
AAAAGCATCG AGGAAGTCTC CCCTCAAAAA TCTATCAAAG ATTTGGTTGG CGGTAAGTCC 
ACTTTGCAAA ACGAAATTCT TGGTGATTTA CAGAAGGAGT TCGGTGCCAC TCCCGAGAAG 
CCAGAGGAGG TTCCATTGGA TGAGCTTGGA GCTATCATGC AGTCAAGCTT TAACGGATCT 
CTTGGTAAAC AATCGTCTTC TCTTATCTCA CGAATGATTT CCTCAAAAAT GCCTGGTGGT 
TTCAATAATT CTGCTGTTCG TGGTTATTTA GGAAACCGTT ATGGTTTGGG TCCTGGTCGT 
TTGGAGTCTG TGCTTTTGTT AGCGCTTACC ATGGAACCTG CATCACGTTT GGGCTCGGAA 
GCTGATGCTA AAGCTTGGCT TGATAGTGTA GCTCAAAAAT ATGCTGCTCG TAATGGTGTT 
ACATTATCTT CTCCTACTGC TGAAGGCGGT TCTTCGTCCG GTTCTGCAGC TGTTATCGAT 
GAAGAAACCT TTAAGAAACT CACCAAGAAT AATACCATGC TTGTTACTCA GCAATTAGAA 
CTATTTGCTC GATACCTCAA TAAAGACCTT CGTGCTGGCC AAAAGGCTCA AGTTGCTGAA 
AAGGTTATTT CCGATACCTT ACGCGCTCAA TTAGATTTAT GGAACGAAGA ACATGGTGAA 
TTTTATGCAT CAGGAATTGC TCCTATTTTT TCGCCTTTAA AAGCTCGCGT TTACGACTCC 
GACTGGAATT GGGCTCGTCA AGATGCTCTT AAGATGTTTT TTGACATTAT CTTTGGTCGT 
CTTAAGCATG TTGATACTGA AATAGTCGCT CGTTGTATTT CTGTTATGAA TAGATCCAAC 
CCTACTTTAC TTGAATTTAT GCAATATCAT ATTGATCATT GTCCCGCCGA AAAGGGTGAA 
ACATATCAAC TTGCTAAAAC CTTGGGCCAA CAGCTAATTG ATAATTGCAA ATCCGTGATA 
GATGCTCCTC CAGTTTTCAA AAATGTGAAT CATCCAACTG CTCCTTCTAC GACGATTGAC 
GAACGTGGTA ATTTGAATTA TGAAGAAATC CCTAGACCAG GTGTTCGCAA ATTAACTCAT 
TACGTTACTG AGATGGCCAA AGGTGGTAAA TTACCAACGG AGTCCAAAAA CAAAGCTAAG 
GTACAAAACG ATTTGGCTCG AATTTATCGC ATTATTAAGT CTCAAAACAA AATGTCTCGT 
TCGTCTAAGT TGCAGATTAA ACAGTTGTAC GGTCAGGTTT TACATGCCCT TTCCCTTCCA 
TTGCCTTCTT CCAACGATGA ACAAACGCCT GTTAAAGAAA CCATTCCTTT CCTTCATATT 
AGGAAGAAGT CCGTTGATGG TAATTGGGAA TTCAACAAGT CATTGACTGG CACTTATTTA 
GATGTTTTAG AATCGGGTGC TAAGAATGGT ATAACATACC AAGACAAATA TGCTCTAGTG 
ACTGGTGCAG GTGCAGGCTC CATTGGTGCT CAGATTGTTG AAGGTCTCCT TGCTGGTGGT 
GCTAAAGTTG TAGTTACTAC ATCCCGGTTT TCGCGCAAGG TTACTGAATT TTATCAATCC 
CTTTACACCC GCCATGGAAG CCGTGGTTCA TGTCTGATCG TGGTTCCATT TAACCAAGGA 
TCTAAGACAG ACGTAGAAGC TCTTATTGAT TATATTTATG ACGAAAAGAA GGGTCTTGGA 
TGGAACTTGG ACTACATTGT TCCTTTCGCT GCCATTCCAG AAAATGGTCG TGAAATTGAT 
GGCATTGATT CTCGTTCCGA GTTTGCTCAC CGTATTATGT TGACAAACAT TTTGAGACTG 
CTTGGCGCCG TCAAAAGTCA AAAGGCCTCT CGTGGTATGG ATACCCGACC CGCTCAAGTT 
ATTTTGCCTC TTTCTCCCAA TCACGGTACC TTTGGAAACG ATGGTTTATA CTCGGAATCT 
AAGTTAGGTT TAGAAACTTT GTTTAACCGT TGGTACTCCG AGTCATGGGC TAATTACCTA 
ACCATTTGTG GGGCTGTCAT TGGTTGGACT CGTGGTACAG GCTTAATGGC ACCTAATAAT 
ATTGTTTCTC AGGGAATCGA AAAATATGGT GTTCGTACTT TTTCGCAGAG TGAGATGGCT 
TTTAACATTT TGGGTTTGAT GTCCCAGAAA GTCGTCGACT TGTGTCAATC TGAACCAATT 
TATGCCAACC TTAACGGTGG TCTTGAGCTT TTACCTGATC TCAAGGACCT TTCCACTCGT 
TTGCGTACCG AATTGTTAGA AACTGCCGAA ATCCGCCGCG CTGTTGCCGC AGAGACTGCC 
TTTGATCATA GCATTACCAA CGGACCTGAC TCTGAAGCAG TTTTCCAGAA AACTGCCATT 
CAGCCTAGGG CCAATCTTAA ATTTAATTTC CCCAAATTGA AACCTTATGA AGCCCTTTCT 
CATTTATCTG ATCTTCGTGG AATGGTTGAT TTAGAAAAAG TTCCTGTTGT TACTGGTTTT 
TCCGAAGTAG GTCCATGGGG TAACTCTCGT ACTAGATGGG ATATGGAGTG TTATGGTGAG 
TTTTCACTAG AAGGATGTGT CGAAATTGCT TGGATTATGG GATTAATTAA AAACTTCAAT 
GGCAAGGGCA AAGACGGCAA GCCCTATTCA GGTTGGGTTG ATACAAAGAC CGGTGAACCT 
GTGGACGACA AAGACGTTAA AGCTAAGTAT GAGAAGTATA TACTGGAGCA TTGCGGTATC 
CGTATTATTG AAGCTGAACT CTTCCATGGA TATAATCCTG AAAAGAAAGA GCTTTTGCAA 
GAAGTTGTTA TTGATCATGA CTTAGAGCCT TTTGAAGCAT CCAAAGAGGC TGCTCATGAG 
TTCAAGCTTC GTCATGGTGA TCAAGTTGAA ATTTTTGAAA TTCCTGATTC TACCGAATGG 
TCCGTACGCT TCAAGCGCGG TACAAGTATG CTAATTCCTA AGGCTTTGCG CTTTGATCGA 
TTTGTTGCTG GCCAGATTCC ACTTGGTTGG GATCCCAAAC GTTATGGCAT TCCTGACGAT 
ATTATTTCTC AAGTTGACCC TACAACTTTG TACGTTTTAG TGTCTACTGT AGAAGCTCTG 
GTTGCATCAG GTATTACAGA TCCTTATGAA TGCTATAAGT ATATTCACGT ATCTGAACTT 
GGTAATACAG TTGGTTCTGG TATTGGTGGT ATGTCTGCTC TTCGTGGAAT GTACAAGGAC 
CGCTGGACTG ATAAACCTGT TCAAAAAGAT ATTTTACAAG AATCATTCAT TAACACTGCC 
AATGCTTGGA TTAACATGCT TTTGCTCTCT GCCTCTGGTC CTATTAAGAC TCCTGTTGGT 
GCTTGCGCTA CCGCTGTCGA ATCTGTTGAT GCAGCTGTCG ACTTGATCAC TTCTGGTAAG 
GCCAGGATAT GTATTAGCGG TGGTTATGAC GACTTTTCAG AAGAAGGTTC ATACGAGTTT 
GCGAACATGG GTGCTACATC AAATGCTGCT AAGGAAACAG AAAGGGGACG TACTCCTCAA 
GAAATGTCTC GTCCTGCTAC TTCTACTCGT GATGGATTTA TGGAGTCTCA AGGTGCTGGT 
GTACAGATTA TCATGCAAGC AAAGCTTGCT ATTGAGATGG GTGTCCCTAT ACATGGTATT 
GTTGGTTATG TTTCCACAGC TATGGATAAA CAAGGTCGTT CGGTTCCTGC CCCTGGGCAA 
GGTATTTTGA CTGGTGCTCG TGAAATCGCG ACTAAGACAC CCCTTCCCAT AGTTGACCTT 
AAATTCCGTT CTCGTCAACT CCAACGCCGC CGTTCTCAAA TTGGTGAATG GGCCGAACGC 
GAGTATCTTT ATTTAGAAGA AGAACTTGAT GCGATGAAGG TTCAAAATCC TGACTTGGAT 
TTAGAGGCTT ACCGTATAGA GCGTATCAAC GTTATTAAGG AGGAGGTTGT TCGACAAGAA 
AAGGAGGCGC TCAATACTTT TGGAAATGAA TTTTGGAAAC GTGATCCTAC TATTGCTCCT 
ATCCGTGGTG CATTAGCTGT TTGGGGTCTT ACTATTGACG ATTTGGGCGT TGCATCATTC 
CATGGTACCT CTACCAAAGC CAATGAGAAG AATGAATGCG ATGTCATTGA CAGTCAGTTA 
ACACATCTCG GACGCTCTAA GGGTAACGCT GTGTACGGTG TTTTCCAGAA ATATCTCACT 
GGACATAGCA AGGGTGGTGC TGGAGCTTGG ATGCTCAACG GAGCTCTCCA AATTCTTCGC 
TCTGGGTTTG TTCCGGGTAA TCGTAACGCC GATAACATTG ATGAGTATCT AGCACGATTC 
GACCGGGTTA TGTTCCCTAG TGAAGGTATA CAAACTGATG GCATAAAGGC AGCATCTGTT 
ACTGCATTTG GTTTTGGACA AGTTGGTGGA CAAGTTATAG TTATCCATCC TGATTACATT 
TACGGTGTGA TTGATGAGGC TACTTATAAT GCTTACAAAG CTAAAACTGC TGCTCGTTAT 
AAGGCATCTT ATCGTTACAC CCACGATGCG CTGGTTTACA ACAATTTGGT CCGCGCCAAG 
GATTCTCCTC CTTACACCAA AGAACAAGAG AAAGCCGTTT ATCTCAATCC TTTGGCACGC 
GCTTCGAAGA GCAAAGCTGG CACTTGGACT TTCCCTGCCA CACTGCCTGC TGAATCCGAC 
ATTTCTAAAA CCAACGAAAC TACACGTACT CTACAAAGCC TAACAACCTC ATTGACCAAC 
TCCAATGAAA ATGTTGGCGT GGATGTTGAA CTTGTATCAG CGATTAGCAT TGATAATGAG 
ACCTTTATAG AAAGGAATTT TACTGATACC GAGCGAAAGT ACTGTTTTGC AGCTCCTAAT 
CCCCAAGCTA GCTTTGCCGG ACGTTGGTCA GCCAAAGAGG CTGTCTTTAA GTCTTTGGGT 
ATTTCCGGTA AAGGCGCTGC AGCTCCATTG AAGGATATCG AAATTATTTC TTCAGAGTCT 
GGTGCTCCTG AAGTAGTTTT GCACGGAGAG GCTGCGAAGG CTGCAACGAC CGCCGGTGTG 
AAGAGTGTTT CCGTCAGTAT TTCCCACGAT GATAATCAAA GTGTCAGTGT TGCTTTGGCT 
CACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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fas2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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