fas1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	fas1
Entrez Gene ID	2543497
Full Name	fatty acid synthase beta subunit Fas1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019791.2	NP_594370.1	fatty acid synthase beta subunit Fas1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B02717g	XP_451653.1
Magnaporthe oryzae (rice blast fungus)	MGG_04118	XP_003719712.1
Saccharomyces cerevisiae (baker's yeast)	FAS1	NP_012739.1
Eremothecium gossypii	AGOS_AER085C	NP_984945.2
Neurospora crassa	NCU07307	XP_962465.1
Schizosaccharomyces pombe (fission yeast)	fas1	NP_594370.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following fas1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fas1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103074	NM_001019791.2 Latest version!	Schizosaccharomyces pombe fatty acid synthase beta subunit Fas1 (fas1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fas1 ( NM_001019791.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594370.1 Schizosaccharomyces pombe fatty acid synthase beta subunit Fas1 (fas1), mRNA.

Price & Availability↑

CloneID	OSc103074
Clone ID Related Accession (Same CDS sequence)	NM_001019791.2
Accession Version	NM_001019791.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	fatty acid synthase beta subunit Fas1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019791.1.

ATGGTTGAGG CTGAACAAGT TCATCAGTCG CTGAGGTCGT TGGTTCTTTC TTATGCTCAT 
TTTTCGCCTT CCATTCTAAT TCCTGCTTCA CAATACTTGT TGGCTGCTCA GCTTCGCGAT 
GAATTCCTGT CCCTTCATCC CGCCCCTTCA GCCGAGTCGG TGGAGAAGGA AGGCGCTGAA 
CTTGAGTTTG AACATGAACT CCACTTACTA GCTGGTTTCC TCGGTTTAAT TGCCGCCAAA 
GAGGAAGAGA CTCCTGGGCA ATATACCCAG CTCTTAAGAA TTATTACTCT TGAATTCGAA 
CGCACCTTCT TAGCCGGTAA TGAGGTTCAT GCCGTCGTTC ATAGTTTGGG ATTGAACATT 
CCTGCGCAAA AAGACGTCGT TCGTTTCTAC TACCACTCTT GTGCCTTGAT TGGTCAGACC 
ACAAAATTCC ATGGATCCGC TTTGTTAGAC GAGTCCTCGG TAAAATTGGC GGCCATTTTT 
GGTGGACAAG GCTATGAAGA TTATTTTGAC GAATTGATTG AACTTTATGA AGTGTATGCT 
CCTTTTGCAG CTGAACTGAT CCAGGTGCTC TCTAAACATC TTTTTACCCT TTCTCAAAAT 
GAACAGGCAT CAAAAGTTTA TTCCAAAGGA CTTAATGTAC TTGACTGGCT CGCTGGCGAG 
CGACCGGAAC GTGACTATTT GGTCAGTGCT CCTGTCTCCC TTCCCCTAGT TGGTCTCACA 
CAGCTCGTGC ACTTCTCCGT AACCGCCCAA ATCTTGGGTT TGAACCCTGG AGAGCTGGCT 
TCTCGCTTCA GCGCAGCTTC TGGTCATTCA CAGGGCATTG TTGTTGCTGC TGCTGTTTCC 
GCTTCTACTG ATTCAGCTTC TTTTATGGAA AATGCCAAAG TTGCTTTGAC TACTTTGTTT 
TGGATTGGTG TTCGCTCTCA ACAGACTTTC CCAACAACTA CGCTTCCCCC TTCAGTCGTT 
GCTGATTCGC TAGCTTCATC TGAAGGAAAC CCCACTCCCA TGTTAGCTGT TCGTGATTTA 
CCCATTGAGA CTCTGAACAA GCATATTGAG ACCACCAATA CTCACCTACC TGAAGACAGG 
AAGGTTTCTC TTAGTTTGGT TAATGGTCCT CGCAGTTTTG TTGTCAGTGG ACCTGCTCGC 
TCTTTGTACG GTTTGAACCT TTCCTTGCGC AAAGAGAAAG CTGATGGACA AAATCAATCT 
CGTATCCCTC ATTCAAAGCG TAAGCTTCGC TTTATCAACC GATTCCTTAG CATTTCTGTT 
CCTTTCCATA GCCCCTACTT GGCTCCTGTT CGTTCTTTAT TAGAAAAGGA TTTGCAAGGT 
CTCCAATTCT CCGCTTTGAA GGTTCCAGTC TATTCTACTG ACGATGCTGG TGATTTAAGA 
TTTGAACAAC CTTCCAAGCT TTTATTAGCT CTTGCGGTAA TGATTACTGA AAAGGTTGTT 
CATTGGGAAG AAGCTTGTGG TTTCCCCGAT GTCACTCATA TCATTGACTT CGGACCTGGT 
GGCATTTCAG GCGTTGGTTC GTTAACACGT GCCAACAAGG ATGGACAGGG CGTACGTGTG 
ATTGTTGCTG ATTCCTTTGA AAGCTTGGAT ATGGGTGCTA AATTTGAAAT TTTCGATCGT 
GATGCTAAGT CAATCGAGTT TGCTCCTAAC TGGGTCAAGT TGTATAGTCC CAAGCTTGTC 
AAGAATAAGT TGGGTCGTGT ATACGTTGAT ACTAGATTGT CTCGTATGCT AGGTCTCCCT 
CCCTTGTGGG TTGCAGGCAT GACTCCTACT AGCGTTCCTT GGCAATTCTG TTCTGCCATT 
GCGAAAGCTG GCTTTACCTA CGAGCTAGCC GGTGGTGGTT ACTTTGATCC AAAAATGATG 
CGCGAAGCCA TCCACAAACT TTCTCTCAAT ATTCCACCCG GTGCTGGTAT TTGTGTTAAC 
GTTATCTACA TTAATCCCCG TACTTATGCC TGGCAGATTC CTTTGATTCG TGATATGGTT 
GCTGAGGGTT ATCCCATTCG TGGCGTTACT ATTGCCGCTG GTATTCCTTC ATTGGAAGTT 
GCTAATGAGT TGATTTCTAC ATTAGGTGTT CAATATCTTT GCCTCAAGCC TGGATCAGTT 
GAAGCTGTCA ACGCTGTCAT TAGCATTGCC AAGGCTAACC CCACTTTCCC CATTGTTTTG 
CAATGGACTG GTGGTCGTGC AGGTGGCCAT CACTCGTTTG AAGACTTTCA TTCTCCCATT 
TTGTTGACAT ATTCTGCAAT CAGACGCTGC GATAACATTG TCCTCATCGC CGGTAGTGGT 
TTCGGTGGAG CTGATGATAC TGAGCCTTAT TTGACTGGTG AGTGGAGTGC TGCATTTAAA 
CTTCCTCCTA TGCCTTTTGA CGGTATATTG TTCGGCTCAC GTTTGATGGT AGCTAAGGAA 
GCACATACGT CTTTGGCTGC GAAGGAGGCT ATTGTAGCTG CTAAAGGTGT TGATGACTCG 
GAGTGGGAGA AGACTTATGA CGGCCCTACT GGTGGAATTG TCACAGTATT ATCCGAACTT 
GGTGAGCCTA TCCACAAGCT TGCAACACGT GGTATCATGT TTTGGAAAGA GCTCGATGAC 
ACTATATTTT CGCTTCCTCG TCCTAAACGT CTTCCAGCCT TGCTTGCTAA GAAACAGTAC 
ATTATTAAAC GACTTAATGA CGATTTCCAA AAAGTGTATT TCCCTGCACA TATCGTAGAA 
CAAGTATCTC CTGAAAAATT CAAGTTTGAG GCTGTTGATA GCGTTGAGGA TATGACTTAT 
GCTGAACTCT TGTATCGTGC AATTGATTTG ATGTACGTTA CGAAAGAAAA GAGATGGATT 
GACGTTACTT TACGTACTTT TACTGGTAAA TTGATGCGCC GTATTGAAGA AAGATTTACC 
CAGGATGTTG GAAAGACAAC TCTTATTGAA AATTTTGAAG ACCTTAATGA TCCATATCCT 
GTGGCTGCTC GTTTCCTCGA TGCTTACCCT GAAGCCAGTA CTCAAGATCT CAACACTCAA 
GATGCTCAAT TTTTTTACTC TCTTTGCTCC AATCCTTTCC AGAAACCAGT TCCATTCATT 
CCCGCAATCG ATGATACTTT TGAATTTTAC TTCAAGAAAG ATTCCCTTTG GCAGAGCGAA 
GATTTGGCTG CCGTTGTTGG TGAAGATGTT GGCCGTGTTG CCATCCTTCA AGGACCCATG 
GCTGCCAAGC ACTCGACAAA GGTTAATGAA CCCGCTAAAG AACTTCTTGA TGGTATTAAT 
GAAACCCATA TTCAACATTT TATTAAAAAG TTTTATGCTG GCGATGAAAA GAAAATTCCT 
ATTGTTGAAT ATTTTGGTGG TGTTCCACCT GTCAATGTTT CTCATAAAAG TTTGGAATCT 
GTTTCTGTTA CTGAAGAGGC TGGTTCTAAA GTTTACAAAT TACCAGAAAT TGGCTCTAAC 
AGCGCTTTAC CTTCTAAGAA GTTATGGTTT GAGTTATTGG CTGGTCCTGA ATATACATGG 
TTCAGAGCTA TCTTTACCAC CCAACGTGTA GCTAAAGGAT GGAAGCTGGA ACATAATCCG 
GTACGCCGAA TTTTTGCTCC ACGTTATGGG CAGCGTGCTG TTGTTAAGGG CAAAGATAAT 
GATACTGTTG TTGAATTATA TGAGACTCAA AGTGGAAACT ATGTACTTGC TGCTCGTTTA 
TCTTATGATG GTGAAACTAT CGTTGTCTCT ATGTTTGAAA ACCGTAATGC TTTGAAGAAG 
GAGGTTCATT TGGACTTCTT GTTCAAGTAC GAGCCCTCTG CTGGTTATTC ACCTGTGTCA 
GAGATCCTTG ATGGCAGAAA TGATCGTATC AAGCATTTCT ACTGGGCTTT ATGGTTCGGA 
GAGGAGCCTT ATCCTGAGAA TGCTTCAATT ACCGATACTT TCACTGGCCC TGAAGTGACC 
GTCACTGGAA ACATGATTGA AGATTTTTGC CGTACAGTTG GAAATCATAA TGAGGCCTAC 
ACTAAGAGAG CAATTAGAAA GCGTATGGCC CCTATGGATT TTGCAATTGT TGTTGGATGG 
CAAGCAATTA CCAAGGCTAT TTTCCCGAAG GCTATTGACG GCGATTTGCT TAGATTGGTT 
CATCTGTCTA ACTCTTTCCG TATGGTTGGT AGCCATTCAT TAATGGAGGG AGACAAGGTA 
ACCACGTCTG CTTCTATCAT TGCTATTTTG AACAACGATT CCGGTAAAAC CGTCACTGTC 
AAGGGTACTG TTTACCGTGA TGGAAAGGAA GTTATTGAAG TTATTAGTCG GTTCTTGTAT 
CGTGGTACTT TTACTGACTT TGAAAATACT TTTGAACATA CCCAAGAAAC CCCAATGCAA 
CTCACCCTTG CTACTCCCAA AGACGTCGCT GTCTTACAGT CTAAGAGTTG GTTCCAGTTG 
CTTGATCCTA GTCAGGATCT TTCTGGATCA ATCCTTACCT TCAGACTCAA CTCTTATGTT 
CGTTTCAAGG ATCAAAAAGT AAAGAGTTCC GTTGAAACTA AAGGTATAGT CCTTTCCGAA 
TTGCCCTCAA AAGCTATCAT CCAAGTCGCT TCTGTCGATT TTCAATCCGT CGATTGCCAT 
GGCAATCCTG TCATCGAATT CTTGAAGCGT AACGGTAAGC CTATTGAGCA GCCCGTCGAG 
TTTGAAAATG GTGGTTACAG TGTAATTCAA GTCATGGATG AGGGATACTC ACCTGTCTTT 
GTTACTCCTC CTACTAACTC GCCTTATGCA GAGGTGTCTG GTGATTACAA TCCTATTCAT 
GTTTCTCCTA CATTCGCTGC ATTTGTTGAA CTTCCTGGTA CTCATGGTAT TACCCATGGT 
ATGTATACTT CTGCTGCTGC TCGTAGATTT GTTGAAACTT ATGCTGCACA AAACGTTCCT 
GAGCGTGTTA AGCATTATGA GGTTACATTT GTGAATATGG TTCTTCCTAA TACTGAGCTT 
ATCACTAAAT TGTCACATAC CGGTATGATT AATGGACGTA AGATTATCAA GGTTGAAGTC 
CTCAACCAAG AAACTTCAGA ACCTGTTCTT GTAGGTACTG CTGAAGTCGA GCAACCTGTT 
TCTGCATATG TTTTCACCGG TCAGGGTTCT CAAGAACAGG GTATGGGTAT GGATCTATAT 
GCTTCAAGTC CCGTAGCTCG TAAGATTTGG GACAGTGCCG ACAAGCACTT CTTAACGAAC 
TATGGATTCT CCATTATTGA CATTGTCAAA CATAATCCCC ATAGCATCAC CATTCACTTT 
GGTGGTTCTA AGGGTAAAAA GATTCGTGAT AACTATATGG CTATGGCATA CGAAAAGCTC 
ATGGAAGACG GAACTAGCAA AGTTGTCCCA GTATTTGAAA CCATTACTAA GGACTCTACC 
TCGTTTAGTT TCACTCATCC TTCCGGCTTA TTGTCAGCTA CTCAATTTAC TCAACCTGCA 
CTGACATTGA TGGAGAAGTC GGCATTTGAA GACATGCGTT CTAAAGGTTT AGTTCAAAAC 
GACTGTGCCT TTGCTGGTCA TTCTTTGGGT GAATATTCTG CTTTGTCCGC TATGGGTGAT 
GTTTTGTCTA TTGAGGCATT GGTAGACTTA GTATTTTTGC GTGGCTTAAC TATGCAAAAC 
GCTGTTCATC GTGATGAATT GGGACGTTCT GATTATGGTA TGGTTGCAGC AAACCCTTCA 
CGAGTGTCAG CTTCCTTCAC CGATGCTGCT TTGCGTTTTA TCGTTGACCA TATTGGCCAA 
CAGACCAACT TGTTGCTTGA GATTGTCAAC TATAACGTTG AGAATCAACA ATACGTTGTT 
AGTGGTAACT TGTTGAGTTT GTCCACTTTA GGTCATGTAC TTAACTTTTT GAAGGTCCAA 
AAGATCGATT TTGAAAAACT TAAGGAAACT TTGACTATTG AACAATTGAA AGAACAACTC 
ACCGATATCG TTGAAGCATG TCATGCTAAG ACTTTGGAGC AACAAAAGAA GACTGGCCGC 
ATTGAGCTGG AACGTGGATA TGCCACAATT CCCCTCAAAA TTGATGTCCC CTTCCACTCC 
TCATTCTTGC GTGGAGGTGT AAGGATGTTC AGAGAGTATT TGGTGAAGAA GATCTTCCCT 
CACCAAATTA ACGTTGCCAA GCTTCGCGGC AAATATATTC CTAACTTGAC TGCCAAGCCT 
TTTGAGATTA GCAAAGAGTA TTTCCAGAAT GTATATGATC TAACTGGTTC CCAACGAATT 
AAAAAGATTT TGCAAAACTG GGATGAATAT GAGTCATCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594370.1
CDS	469..6690
Translation	MVEAEQVHQSLRSLVLSYAHFSPSILIPASQYLLAAQLRDEFLSLHPAPSAESVEKEGAELEFEHELHLLAGFLGLIAAKEEETPGQYTQLLRIITLEFERTFLAGNEVHAVVHSLGLNIPAQKDVVRFYYHSCALIGQTTKFHGSALLDESSVKLAAIFGGQGYEDYFDELIELYEVYAPFAAELIQVLSKHLFTLSQNEQASKVYSKGLNVLDWLAGERPERDYLVSAPVSLPLVGLTQLVHFSVTAQILGLNPGELASRFSAASGHSQGIVVAAAVSASTDSASFMENAKVALTTLFWIGVRSQQTFPTTTLPPSVVADSLASSEGNPTPMLAVRDLPIETLNKHIETTNTHLPEDRKVSLSLVNGPRSFVVSGPARSLYGLNLSLRKEKADGQNQSRIPHSKRKLRFINRFLSISVPFHSPYLAPVRSLLEKDLQGLQFSALKVPVYSTDDAGDLRFEQPSKLLLALAVMITEKVVHWEEACGFPDVTHIIDFGPGGISGVGSLTRANKDGQGVRVIVADSFESLDMGAKFEIFDRDAKSIEFAPNWVKLYSPKLVKNKLGRVYVDTRLSRMLGLPPLWVAGMTPTSVPWQFCSAIAKAGFTYELAGGGYFDPKMMREAIHKLSLNIPPGAGICVNVIYINPRTYAWQIPLIRDMVAEGYPIRGVTIAAGIPSLEVANELISTLGVQYLCLKPGSVEAVNAVISIAKANPTFPIVLQWTGGRAGGHHSFEDFHSPILLTYSAIRRCDNIVLIAGSGFGGADDTEPYLTGEWSAAFKLPPMPFDGILFGSRLMVAKEAHTSLAAKEAIVAAKGVDDSEWEKTYDGPTGGIVTVLSELGEPIHKLATRGIMFWKELDDTIFSLPRPKRLPALLAKKQYIIKRLNDDFQKVYFPAHIVEQVSPEKFKFEAVDSVEDMTYAELLYRAIDLMYVTKEKRWIDVTLRTFTGKLMRRIEERFTQDVGKTTLIENFEDLNDPYPVAARFLDAYPEASTQDLNTQDAQFFYSLCSNPFQKPVPFIPAIDDTFEFYFKKDSLWQSEDLAAVVGEDVGRVAILQGPMAAKHSTKVNEPAKELLDGINETHIQHFIKKFYAGDEKKIPIVEYFGGVPPVNVSHKSLESVSVTEEAGSKVYKLPEIGSNSALPSKKLWFELLAGPEYTWFRAIFTTQRVAKGWKLEHNPVRRIFAPRYGQRAVVKGKDNDTVVELYETQSGNYVLAARLSYDGETIVVSMFENRNALKKEVHLDFLFKYEPSAGYSPVSEILDGRNDRIKHFYWALWFGEEPYPENASITDTFTGPEVTVTGNMIEDFCRTVGNHNEAYTKRAIRKRMAPMDFAIVVGWQAITKAIFPKAIDGDLLRLVHLSNSFRMVGSHSLMEGDKVTTSASIIAILNNDSGKTVTVKGTVYRDGKEVIEVISRFLYRGTFTDFENTFEHTQETPMQLTLATPKDVAVLQSKSWFQLLDPSQDLSGSILTFRLNSYVRFKDQKVKSSVETKGIVLSELPSKAIIQVASVDFQSVDCHGNPVIEFLKRNGKPIEQPVEFENGGYSVIQVMDEGYSPVFVTPPTNSPYAEVSGDYNPIHVSPTFAAFVELPGTHGITHGMYTSAAARRFVETYAAQNVPERVKHYEVTFVNMVLPNTELITKLSHTGMINGRKIIKVEVLNQETSEPVLVGTAEVEQPVSAYVFTGQGSQEQGMGMDLYASSPVARKIWDSADKHFLTNYGFSIIDIVKHNPHSITIHFGGSKGKKIRDNYMAMAYEKLMEDGTSKVVPVFETITKDSTSFSFTHPSGLLSATQFTQPALTLMEKSAFEDMRSKGLVQNDCAFAGHSLGEYSALSAMGDVLSIEALVDLVFLRGLTMQNAVHRDELGRSDYGMVAANPSRVSASFTDAALRFIVDHIGQQTNLLLEIVNYNVENQQYVVSGNLLSLSTLGHVLNFLKVQKIDFEKLKETLTIEQLKEQLTDIVEACHAKTLEQQKKTGRIELERGYATIPLKIDVPFHSSFLRGGVRMFREYLVKKIFPHQINVAKLRGKYIPNLTAKPFEISKEYFQNVYDLTGSQRIKKILQNWDEYESS

Target ORF information:

RefSeq Version	NM_001019791.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe fatty acid synthase beta subunit Fas1 (fas1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019791.2

ORF Insert Sequence:

ATGGTTGAGG CTGAACAAGT TCATCAGTCG CTGAGGTCGT TGGTTCTTTC TTATGCTCAT 
TTTTCGCCTT CCATTCTAAT TCCTGCTTCA CAATACTTGT TGGCTGCTCA GCTTCGCGAT 
GAATTCCTGT CCCTTCATCC CGCCCCTTCA GCCGAGTCGG TGGAGAAGGA AGGCGCTGAA 
CTTGAGTTTG AACATGAACT CCACTTACTA GCTGGTTTCC TCGGTTTAAT TGCCGCCAAA 
GAGGAAGAGA CTCCTGGGCA ATATACCCAG CTCTTAAGAA TTATTACTCT TGAATTCGAA 
CGCACCTTCT TAGCCGGTAA TGAGGTTCAT GCCGTCGTTC ATAGTTTGGG ATTGAACATT 
CCTGCGCAAA AAGACGTCGT TCGTTTCTAC TACCACTCTT GTGCCTTGAT TGGTCAGACC 
ACAAAATTCC ATGGATCCGC TTTGTTAGAC GAGTCCTCGG TAAAATTGGC GGCCATTTTT 
GGTGGACAAG GCTATGAAGA TTATTTTGAC GAATTGATTG AACTTTATGA AGTGTATGCT 
CCTTTTGCAG CTGAACTGAT CCAGGTGCTC TCTAAACATC TTTTTACCCT TTCTCAAAAT 
GAACAGGCAT CAAAAGTTTA TTCCAAAGGA CTTAATGTAC TTGACTGGCT CGCTGGCGAG 
CGACCGGAAC GTGACTATTT GGTCAGTGCT CCTGTCTCCC TTCCCCTAGT TGGTCTCACA 
CAGCTCGTGC ACTTCTCCGT AACCGCCCAA ATCTTGGGTT TGAACCCTGG AGAGCTGGCT 
TCTCGCTTCA GCGCAGCTTC TGGTCATTCA CAGGGCATTG TTGTTGCTGC TGCTGTTTCC 
GCTTCTACTG ATTCAGCTTC TTTTATGGAA AATGCCAAAG TTGCTTTGAC TACTTTGTTT 
TGGATTGGTG TTCGCTCTCA ACAGACTTTC CCAACAACTA CGCTTCCCCC TTCAGTCGTT 
GCTGATTCGC TAGCTTCATC TGAAGGAAAC CCCACTCCCA TGTTAGCTGT TCGTGATTTA 
CCCATTGAGA CTCTGAACAA GCATATTGAG ACCACCAATA CTCACCTACC TGAAGACAGG 
AAGGTTTCTC TTAGTTTGGT TAATGGTCCT CGCAGTTTTG TTGTCAGTGG ACCTGCTCGC 
TCTTTGTACG GTTTGAACCT TTCCTTGCGC AAAGAGAAAG CTGATGGACA AAATCAATCT 
CGTATCCCTC ATTCAAAGCG TAAGCTTCGC TTTATCAACC GATTCCTTAG CATTTCTGTT 
CCTTTCCATA GCCCCTACTT GGCTCCTGTT CGTTCTTTAT TAGAAAAGGA TTTGCAAGGT 
CTCCAATTCT CCGCTTTGAA GGTTCCAGTC TATTCTACTG ACGATGCTGG TGATTTAAGA 
TTTGAACAAC CTTCCAAGCT TTTATTAGCT CTTGCGGTAA TGATTACTGA AAAGGTTGTT 
CATTGGGAAG AAGCTTGTGG TTTCCCCGAT GTCACTCATA TCATTGACTT CGGACCTGGT 
GGCATTTCAG GCGTTGGTTC GTTAACACGT GCCAACAAGG ATGGACAGGG CGTACGTGTG 
ATTGTTGCTG ATTCCTTTGA AAGCTTGGAT ATGGGTGCTA AATTTGAAAT TTTCGATCGT 
GATGCTAAGT CAATCGAGTT TGCTCCTAAC TGGGTCAAGT TGTATAGTCC CAAGCTTGTC 
AAGAATAAGT TGGGTCGTGT ATACGTTGAT ACTAGATTGT CTCGTATGCT AGGTCTCCCT 
CCCTTGTGGG TTGCAGGCAT GACTCCTACT AGCGTTCCTT GGCAATTCTG TTCTGCCATT 
GCGAAAGCTG GCTTTACCTA CGAGCTAGCC GGTGGTGGTT ACTTTGATCC AAAAATGATG 
CGCGAAGCCA TCCACAAACT TTCTCTCAAT ATTCCACCCG GTGCTGGTAT TTGTGTTAAC 
GTTATCTACA TTAATCCCCG TACTTATGCC TGGCAGATTC CTTTGATTCG TGATATGGTT 
GCTGAGGGTT ATCCCATTCG TGGCGTTACT ATTGCCGCTG GTATTCCTTC ATTGGAAGTT 
GCTAATGAGT TGATTTCTAC ATTAGGTGTT CAATATCTTT GCCTCAAGCC TGGATCAGTT 
GAAGCTGTCA ACGCTGTCAT TAGCATTGCC AAGGCTAACC CCACTTTCCC CATTGTTTTG 
CAATGGACTG GTGGTCGTGC AGGTGGCCAT CACTCGTTTG AAGACTTTCA TTCTCCCATT 
TTGTTGACAT ATTCTGCAAT CAGACGCTGC GATAACATTG TCCTCATCGC CGGTAGTGGT 
TTCGGTGGAG CTGATGATAC TGAGCCTTAT TTGACTGGTG AGTGGAGTGC TGCATTTAAA 
CTTCCTCCTA TGCCTTTTGA CGGTATATTG TTCGGCTCAC GTTTGATGGT AGCTAAGGAA 
GCACATACGT CTTTGGCTGC GAAGGAGGCT ATTGTAGCTG CTAAAGGTGT TGATGACTCG 
GAGTGGGAGA AGACTTATGA CGGCCCTACT GGTGGAATTG TCACAGTATT ATCCGAACTT 
GGTGAGCCTA TCCACAAGCT TGCAACACGT GGTATCATGT TTTGGAAAGA GCTCGATGAC 
ACTATATTTT CGCTTCCTCG TCCTAAACGT CTTCCAGCCT TGCTTGCTAA GAAACAGTAC 
ATTATTAAAC GACTTAATGA CGATTTCCAA AAAGTGTATT TCCCTGCACA TATCGTAGAA 
CAAGTATCTC CTGAAAAATT CAAGTTTGAG GCTGTTGATA GCGTTGAGGA TATGACTTAT 
GCTGAACTCT TGTATCGTGC AATTGATTTG ATGTACGTTA CGAAAGAAAA GAGATGGATT 
GACGTTACTT TACGTACTTT TACTGGTAAA TTGATGCGCC GTATTGAAGA AAGATTTACC 
CAGGATGTTG GAAAGACAAC TCTTATTGAA AATTTTGAAG ACCTTAATGA TCCATATCCT 
GTGGCTGCTC GTTTCCTCGA TGCTTACCCT GAAGCCAGTA CTCAAGATCT CAACACTCAA 
GATGCTCAAT TTTTTTACTC TCTTTGCTCC AATCCTTTCC AGAAACCAGT TCCATTCATT 
CCCGCAATCG ATGATACTTT TGAATTTTAC TTCAAGAAAG ATTCCCTTTG GCAGAGCGAA 
GATTTGGCTG CCGTTGTTGG TGAAGATGTT GGCCGTGTTG CCATCCTTCA AGGACCCATG 
GCTGCCAAGC ACTCGACAAA GGTTAATGAA CCCGCTAAAG AACTTCTTGA TGGTATTAAT 
GAAACCCATA TTCAACATTT TATTAAAAAG TTTTATGCTG GCGATGAAAA GAAAATTCCT 
ATTGTTGAAT ATTTTGGTGG TGTTCCACCT GTCAATGTTT CTCATAAAAG TTTGGAATCT 
GTTTCTGTTA CTGAAGAGGC TGGTTCTAAA GTTTACAAAT TACCAGAAAT TGGCTCTAAC 
AGCGCTTTAC CTTCTAAGAA GTTATGGTTT GAGTTATTGG CTGGTCCTGA ATATACATGG 
TTCAGAGCTA TCTTTACCAC CCAACGTGTA GCTAAAGGAT GGAAGCTGGA ACATAATCCG 
GTACGCCGAA TTTTTGCTCC ACGTTATGGG CAGCGTGCTG TTGTTAAGGG CAAAGATAAT 
GATACTGTTG TTGAATTATA TGAGACTCAA AGTGGAAACT ATGTACTTGC TGCTCGTTTA 
TCTTATGATG GTGAAACTAT CGTTGTCTCT ATGTTTGAAA ACCGTAATGC TTTGAAGAAG 
GAGGTTCATT TGGACTTCTT GTTCAAGTAC GAGCCCTCTG CTGGTTATTC ACCTGTGTCA 
GAGATCCTTG ATGGCAGAAA TGATCGTATC AAGCATTTCT ACTGGGCTTT ATGGTTCGGA 
GAGGAGCCTT ATCCTGAGAA TGCTTCAATT ACCGATACTT TCACTGGCCC TGAAGTGACC 
GTCACTGGAA ACATGATTGA AGATTTTTGC CGTACAGTTG GAAATCATAA TGAGGCCTAC 
ACTAAGAGAG CAATTAGAAA GCGTATGGCC CCTATGGATT TTGCAATTGT TGTTGGATGG 
CAAGCAATTA CCAAGGCTAT TTTCCCGAAG GCTATTGACG GCGATTTGCT TAGATTGGTT 
CATCTGTCTA ACTCTTTCCG TATGGTTGGT AGCCATTCAT TAATGGAGGG AGACAAGGTA 
ACCACGTCTG CTTCTATCAT TGCTATTTTG AACAACGATT CCGGTAAAAC CGTCACTGTC 
AAGGGTACTG TTTACCGTGA TGGAAAGGAA GTTATTGAAG TTATTAGTCG GTTCTTGTAT 
CGTGGTACTT TTACTGACTT TGAAAATACT TTTGAACATA CCCAAGAAAC CCCAATGCAA 
CTCACCCTTG CTACTCCCAA AGACGTCGCT GTCTTACAGT CTAAGAGTTG GTTCCAGTTG 
CTTGATCCTA GTCAGGATCT TTCTGGATCA ATCCTTACCT TCAGACTCAA CTCTTATGTT 
CGTTTCAAGG ATCAAAAAGT AAAGAGTTCC GTTGAAACTA AAGGTATAGT CCTTTCCGAA 
TTGCCCTCAA AAGCTATCAT CCAAGTCGCT TCTGTCGATT TTCAATCCGT CGATTGCCAT 
GGCAATCCTG TCATCGAATT CTTGAAGCGT AACGGTAAGC CTATTGAGCA GCCCGTCGAG 
TTTGAAAATG GTGGTTACAG TGTAATTCAA GTCATGGATG AGGGATACTC ACCTGTCTTT 
GTTACTCCTC CTACTAACTC GCCTTATGCA GAGGTGTCTG GTGATTACAA TCCTATTCAT 
GTTTCTCCTA CATTCGCTGC ATTTGTTGAA CTTCCTGGTA CTCATGGTAT TACCCATGGT 
ATGTATACTT CTGCTGCTGC TCGTAGATTT GTTGAAACTT ATGCTGCACA AAACGTTCCT 
GAGCGTGTTA AGCATTATGA GGTTACATTT GTGAATATGG TTCTTCCTAA TACTGAGCTT 
ATCACTAAAT TGTCACATAC CGGTATGATT AATGGACGTA AGATTATCAA GGTTGAAGTC 
CTCAACCAAG AAACTTCAGA ACCTGTTCTT GTAGGTACTG CTGAAGTCGA GCAACCTGTT 
TCTGCATATG TTTTCACCGG TCAGGGTTCT CAAGAACAGG GTATGGGTAT GGATCTATAT 
GCTTCAAGTC CCGTAGCTCG TAAGATTTGG GACAGTGCCG ACAAGCACTT CTTAACGAAC 
TATGGATTCT CCATTATTGA CATTGTCAAA CATAATCCCC ATAGCATCAC CATTCACTTT 
GGTGGTTCTA AGGGTAAAAA GATTCGTGAT AACTATATGG CTATGGCATA CGAAAAGCTC 
ATGGAAGACG GAACTAGCAA AGTTGTCCCA GTATTTGAAA CCATTACTAA GGACTCTACC 
TCGTTTAGTT TCACTCATCC TTCCGGCTTA TTGTCAGCTA CTCAATTTAC TCAACCTGCA 
CTGACATTGA TGGAGAAGTC GGCATTTGAA GACATGCGTT CTAAAGGTTT AGTTCAAAAC 
GACTGTGCCT TTGCTGGTCA TTCTTTGGGT GAATATTCTG CTTTGTCCGC TATGGGTGAT 
GTTTTGTCTA TTGAGGCATT GGTAGACTTA GTATTTTTGC GTGGCTTAAC TATGCAAAAC 
GCTGTTCATC GTGATGAATT GGGACGTTCT GATTATGGTA TGGTTGCAGC AAACCCTTCA 
CGAGTGTCAG CTTCCTTCAC CGATGCTGCT TTGCGTTTTA TCGTTGACCA TATTGGCCAA 
CAGACCAACT TGTTGCTTGA GATTGTCAAC TATAACGTTG AGAATCAACA ATACGTTGTT 
AGTGGTAACT TGTTGAGTTT GTCCACTTTA GGTCATGTAC TTAACTTTTT GAAGGTCCAA 
AAGATCGATT TTGAAAAACT TAAGGAAACT TTGACTATTG AACAATTGAA AGAACAACTC 
ACCGATATCG TTGAAGCATG TCATGCTAAG ACTTTGGAGC AACAAAAGAA GACTGGCCGC 
ATTGAGCTGG AACGTGGATA TGCCACAATT CCCCTCAAAA TTGATGTCCC CTTCCACTCC 
TCATTCTTGC GTGGAGGTGT AAGGATGTTC AGAGAGTATT TGGTGAAGAA GATCTTCCCT 
CACCAAATTA ACGTTGCCAA GCTTCGCGGC AAATATATTC CTAACTTGAC TGCCAAGCCT 
TTTGAGATTA GCAAAGAGTA TTTCCAGAAT GTATATGATC TAACTGGTTC CCAACGAATT 
AAAAAGATTT TGCAAAACTG GGATGAATAT GAGTCATCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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fas1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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