cel-2 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	cel-2
Entrez Gene ID	3878613
Full Name	chain elongation-2
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395933.1	XP_011394235.1	fatty acid synthase beta subunit dehydratase
XM_011395934.1	XP_011394236.1	fatty acid synthase beta subunit dehydratase, variant

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B02717g	XP_451653.1
Magnaporthe oryzae (rice blast fungus)	MGG_04118	XP_003719712.1
Saccharomyces cerevisiae (baker's yeast)	FAS1	NP_012739.1
Eremothecium gossypii	AGOS_AER085C	NP_984945.2
Neurospora crassa	NCU07307	XP_962465.1
Schizosaccharomyces pombe (fission yeast)	fas1	NP_594370.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cel-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cel-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf01152	XM_011395933.1 Latest version!	Neurospora crassa OR74A fatty acid synthase beta subunit dehydratase (cel-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ONf01152	XM_011395934.1 Latest version!	Neurospora crassa OR74A fatty acid synthase beta subunit dehydratase, variant (cel-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cel-2 ( XM_011395933.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394235.1 Neurospora crassa OR74A fatty acid synthase beta subunit dehydratase (cel-2), mRNA.

Price & Availability↑

CloneID	ONf01152
Clone ID Related Accession (Same CDS sequence)	XM_011395933.1 , XM_011395934.1
Accession Version	XM_011395933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	fatty acid synthase beta subunit dehydratase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504).

ATGTACGGTA CCGGTACCGG CGCTCAGACG GGCGTCACCA CCCCCAGGTC GTCTGCCTCT 
CTGCGGCCGC TCACCGTCTC TCACGGGTCC CTCGAAACCT CTTTCTTGAT ACCCACCGGC 
CTTCATTTCC ACGCTACTCA GCTAAAGGAG CGTTTTGCTG CTATCCTTCC CGCCCCTACT 
GACGAGCTCG CGCAAGATGA CGAGCCCTCG TCCATTTTCG AGCTTGTTGC CAGATACATG 
GGCTTTATCG CGACCGAGGT GACACAAGGC GAGGACGACG CTCAGGGTTC ATACGAGGAG 
GTACTCAAAA TCATCCTGAA CGAGTTCGAG AGGGCGTTCC TGCGGGGCAA CGATGTCCAT 
GCCCTGGTCT CTACAATGGA GGGAATCGAC GACAAGAAGC TCGAAGTCAT TCGCTGCTAC 
TACCTCGCGC GATCAGCTAG CAACAGAGCC ATCAAGCCTT ACGAGTCGGC TCTGTTCCGC 
GCCGCTGGTG AAAACGCGGC CAAGATCTTC ACCATCTTTG GTGGCCAGGG AAACATCGAG 
GAGTACTTCG AAGAGCTCCG GGAGCTGCAC CACACCTATC CGGCATTCGT CGGCGAGCTG 
ATCACCACAT CCGCCGAGCT GCTCCAGACC CTGTCCAGCC ACCCTGATGC CGAGAAGATG 
TACCCCAAGG GCCTTGACGT GCTTGGTTGG CTTCAGAGCC CAGAGGCCAC CCCCGACGTC 
GACTACCTTA TCTCTGCCCC CGTCAGCTTC CCCTTGATTG GTTTGGTACA GCTGGCACAC 
TATCAGGTCA CCTGCAAGGT GCTGGGTGTG CATCCTGGCG TCCTTCGTGA GAGAATCAGC 
GGAACGACAG GTCACTCTCA AGGTGTTGTT CTTTCCGCTA TCACTGCGGC CGCGGATTCG 
TGGGAAAGTT TCGAGGAGCA TAGCAAGGCT GCCTTGACCA TCCTCTTCTG GATCGGCGCT 
CGCAGCCAGC AAACCTTCCC TAGAACTTCC CTAGCCCCCT CGATGCTTCA AGACTCGGCT 
GATAATGGCG AGGGTGTTCC TACCCCCATG CTCAGCATTC GAGACCTTCC CCAAGCCGAG 
GTTCAGAAGC ATATCGATCA GACTAATGCC TACCTTCCTG AGGACCAGCA TATTTCCATT 
GCTTTGATCA ACAGCCCCCG GAACATGGTC GTTGCCGGGC CTCCCAGCTC TCTGCACGGT 
CTTAACTTGC AGCTGAGAAA GCTCAAGGCT GCTACTGGTC TTGATCAGAC CAGAATCCCC 
CATACCGAGC GTAAGGTTCG GTTCGTGAAC CGTTTCCTGC CCATCACTGC TCCTTTCCAC 
AGCAAGTACC TCGCCAAGGC TTCCGACCTG ATTGCCGAAG ACCTCAAGGA CGTTCAAATT 
GACTCTCGCA CCCTGGGTAT GCCCGTTTAT AACACTTTCA CGGGCAAGGA TCTTCGGGAG 
GAAGTTGAGG GCAACATTGT TCCTTCCCTT ATCCGCATGA TTACGGGCGA GACCGTCCAC 
TGGGAGAAGG CCACCGTGTT CCAGGATGCC ACCCATATCC TCGATTTTGG CCCTGGCGGT 
ATCTCTGGTC TCGGCATTCT GACTAGCCGT AACAAGGAGG GTACCGGCGT TCGCGTCATT 
CTTGCTGGCA CGGTGAACGG AACTGTCACT GATGTTGGCT ACAAGCCCGA GTTGTTCGAC 
CGTGACGAGG AGCAGGCAGT CAAGTTTGCT GTGAACTGGG TCAAGGAATA CGGTCCTAGA 
CTGGTCAAGA ACTCTGTTGG TCGTACATAC GTTGATACCA AGATGAGCAG ACTTCTTGGC 
CTCCCGCCCT TGTTGGTCGC TGGTATGACT CCTACCACTG TGCCTTGGGA CTTCATTGCC 
GCCACCATGA ATGCTGGCTA CCAGATCGAG CTTGCTGGTG GTGGCTACTA CAATGCCAAG 
ACCATGACCG AGGCCATCTC CAAGATTGAG AAGGCTGTTC CTGCTGGTCG CGGTATCTGC 
GTCAACTTGA TCTATGTCAA CCCTCGCGCC ATGGGCTGGC AGATTCCCCT GCTTGGCAGG 
CTCAGAGCTG AGGGCTATCC CATCGAAGGT CTCACCATCG GTGCTGGCGT TCCTTCCATC 
GAGGTCGCGC AGGAGTATAT CGAAACTCTG GGACTCAAGC ACATCTCTTT CAAGCCTGGT 
TCGGGTGAGG CTATTCAGGC CGTCATCAAC ATCGCCAAGG CCAACCCTAC CTTCCCCGTG 
ATTCTCCAAT GGACCGGTGG CAGAGGTGGT GGTCATCACT CGTTTGAGGA TTTCCATCAC 
CCCATCCTCA ACCTCTATGG CCGCATCCGC CGCCAAGAAA ACATCATTCT CGTTGCCGGT 
AGCGGTTTCG GAGGTGCTGA TGACACTTAC CCCTACCTCA CTGGTGACTG GTCCATCAAG 
TATGGCTATC CCCCCATGCC CTTCGACGGC TGCATGTTCG GTAGTCGTAT GATGGTCGCC 
AAGGAAGCCC ACACCAGCCT GGCTGCCAAG CAGGCTATCG TTGACGCTCC TGGTCTTGAC 
GACAGTGACT GGGAGAAGAC CTACAAGGGC CCAGCTGGCG GTGTCATCAC TGTCCGCTCT 
GAGATGGGTG AGCCCATTCA TAAGCTCGCC ACTCGTGGTG TCCGCTTCTG GGCTGAGATG 
GACCAGAAGA TCTTCAGCCT TCCCAAGGAG AAGAGGGTCG CTGCTCTTAA GGCTAGCCGT 
GATTACATCA TCGAGAAGCT CAACAAGGAC TTCCAAAAGG TCTGGTTTGG TCAGAACAAG 
GAGGGCAAGG CCGTTGATCT TGAGGACATG ACATATGCTG AGATTGTTCG CCGCCTGGTC 
GAGCTTCTCT ACGTCAAGGA TGAGGCTAGG TGGATTGATC AGTCGTACAC CAAGCTTACT 
GGTGACTTTA TCCACCGTAT TGAGGAGCGA TTTACTACCA CGCCTGGCCA GCCTTCCAGA 
CTCCAGAGTT ATGCTGACCT CAAGGAGCCC TACTCTGCTG TTGAGCAGAT TCTCTCGCAC 
TATCCCGAGG CCGAGAAGCA ACTCATCAAC GCTCAGGATG TCCAACATTT CCTCCTGCTC 
TGTCAACGTC GTGGACAGAA GCCCGTCACC TTTGTGCCTG TTCTCGATGA GAACTTCGAG 
TTTTTCTTCA AGAAGGATTC GCTCTGGCAG TCGGAGGATC TTGCGGCTGT CGTTGGCAAG 
GATGTCGGCC GTACCTGTAT TCTTCAGGGT CCCATGGCTG CCAAGCACGC CACTAAGGTG 
GACGAGCCCA TCAAGGACAT CCTCGACGGA ATTCACAACG GTCACATCGC CTTGCTCACC 
CGTGATATGT ATGGCGGCGA TGAGAGCAAG ATCCCTACCA TTGAGTACTT TGGTGGTAAG 
CTGATCGAGT CAGAAATTCC TCTCGATATC GAGGGTCTTA CTGTCTCCTA TGATGAGCAC 
AAGAACACCT ATCGTCTGTC CTCATCGCCT TCCGTTCAAC TTCCTTCGCT GGACGCCTGG 
CTTTCGCTTC TCGCCGGACA CCAAAGAAAC TGGCGTTTCG CCCTTCTCCA GTCCGACGTC 
CTCGTCCAAG GACAAAAATA CCAGACCAAC CCCATGCGCC GCATCTTTGC GCCGGCTCGT 
GGTCTGTTTG TCGAGATTCT ACACCCGAAC AACCCCGAGA AGACGGTCAT TCTCGTCAAG 
GAGCAGCCAC GCCCCAACCG CTACGCTGAG GTCATTGAGG TCAAGCTGGA CGGCAAGAAC 
GAGATTGTAG TCAACATGAT CAAGGATACC ACGGCCCTCG GAAAGCCAGT GCCCCTCACT 
CTCAAGTTTA TCTACAACCC CGAGGCTGGC TATGCTCCTA TTCATGAGGT TATGGATGGT 
CGCAATGATC GTATCAAGGA GTTCTACTGG CGCGCGTGGT TCGGCGATGA GAAGCTCGAC 
CTCGATTCCT CCGTTCATGA AGTCTTCGAC GGCGGCAAGA CCACCATCAC CAGCGAGGCC 
ATCAATGAGT TTGTTCACGC CGTTGGCAAC ACCGGAGAGG CCTTCGTTGA CAGACCCGAC 
AAGGTCATGT ATGCTCCCAT GGACTTTGCT ATCGTTGTTG GCTGGAAGGC TATCACCAAG 
CCTATTTTCC CTCGCACCAT TGACGGTGAC CTGCTCAAGC TCGTCCATTT GTCCAACCAG 
TTCCGCATGA TGCCTGGTGC TGAGCCCCTC AAGAAGGGTG ATGTGGTTTC GACTACTGCT 
CAGATCAACG CTGTCATCAA CCAGGAGTCT GGAAAGATGG TCGAGGTCTG TGGTACCATC 
ACTCGTGACG GTGAGAAGGT CATGGAGGTT ACTTCGCAGT TCTTGTATCG TGGTACCTAC 
ACCGACTTCG AGAACACATT CCAGCGCAAG GTGGAGACCC CTATGCAGCT TCATCTTGCC 
ACCTCCAAGG ACGTGGCCGT CCTTCGCTCC AAGGATTGGT TCATGATGGA TGATGTTGAA 
AACCCTGATA TTGAGCTTCT CGGCCAGACC CTCACCTTCC GTCTCCAGAC CTTGGTTCGC 
TTCAAGAACA AGACTGTCTT CAGCCACGTG GAGACCCAGG GCCAGGTTCT TCTTGAGCTC 
CCGACCAAGG AGATTATCCA GGTTGCCACT GTCAACTACT ACGCTGGAGA ATCTCACGGA 
AATCCCGTCA TCGACTACCT CCAGCGTCAT GGTTCCTCCA TTGAACAGCC GATCAACTTT 
GAGAACCCTA TCCCTCTGAG CGGAAAGACC CCCCTCCAAC TCAAGGCGCC CTCGTCCAAC 
GAGACTTATG CCCGCGTCTC CGGTGATTAT AACCCCATTC ACGTTTCTCG TGTCTTTTCC 
AGCTATGCCA ACCTTCCTGG CACCATCACT CACGGCATGT ACTCGAGTGC CGCGGTGCGC 
AGCTTGGTTG AGACCTGGGC TGCCGAGAAC AAGGTTGGCC GCGTTCGCAG CTTCCACGCC 
TCTCTTACCG GCATGGTTCT GCCCAATGAT GATATCAACG TGAAGTTGCA GCACGTCGGC 
ATGGTTGCTG GCCGGAAGAT TATCAAGGTT GAGGCTAGCA ACAAGGAGAC CGAGGAAAAG 
GTGCTCCTGG GCGAGGCCGA AATTGAGCAG CCTATCACCG CTTACATCTT CACCGGCCAG 
GGCTCGCAGG AGCAGGGCAT GGGTATGGAT CTTTATAACA GCAGCCCTGT GGCCAAGGCT 
GTCTGGGATC GGGCCGACAA GTATCTCATG GACACTTATG GCTTTGCCAT CACCAACATT 
GTCCGGAACA ACCCCAAGGA GCTCACTATT CACTTTGGTG GCCCTCGTGG CAAGGCCATT 
CGCCAGAACT ACATGTCCAT GACTTTCGAG ACTGTGGCTG CCGATGGCTC GATCAAGTCG 
GAGCGCATGT TCAAGGAGAT CGACGAGAAG ACTACCTCTT ACACTTACCG CTCACCCAAC 
GGTCTTCTCT CGGCTACCCA GTTCACGCAG CCCGCTCTTA CTCTAATGGA AAAGGCCAGC 
TTCGAGGACA TGAAGGCCAA GGGACTTGTG CCCCGTGATA GCACCTTCGC TGGTCACTCT 
CTTGGCGAAT ACTCTGCGCT GGCTGCCCTT GCCGATGTCA TGCCCATCGA GTCCCTCGTG 
TCCGTCGTCT TCTACCGTGG TCTCACCATG CAAGTCGCTG TCGAGCGCGA TGAGACAGGC 
CGCTCCAACT ATGGCATGTG CGCTGTCAAC CCCAGCCGTA TCAACAAGAC TTTCACCGAA 
GAGGCCTTGC GGTTCGTGGT CGGTAGCATC GCAGAGACCA CCGGATGGCT TCTTGAAATC 
GTCAACTTCA ACGTCGAGAA CATGCAGTAC GTCTGTGCTG GCGACTTGCG TGCGCTCGAC 
ACCCTTGGCG GTGTGTGCAA CGCTATCAAG ATGATGAAGA TCGACATTGA GAAAATGCGC 
AAGGAGTACT CTGCCGAGAC TGTCAAGGAG CAGCTGGTCG AGATCATCAA GTCGTGCGCC 
AACGACACTC AGGCTAAGCC CACTCCCCTC GTTCTCCAGC GTGGCTTTGC AACTATCCCG 
CTCCCCGGTA TCGATGTGCC CTTCCACTCG ACATTCCTGC GCTCTGGTGT CAAGCCTTTC 
AGAAGCTTCT TGCTCAAGAA GATCAACAAG ACTACCATCG ACCCATCGAA GCTTATTGGC 
AAGTACATTC CCAACGTGAC TGCTAAGCCA TTCGCAATCA CCAAGGAGTA TTTCGAGGAT 
GTCTACCGGT TGACGAACTC CCCTAAGATC CAACAGATCT TGGCAAACTG GGATAAGTAC 
CAGGAGGAGG GTGTCAGTGG AATCCCTGAT GCGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394235.1
CDS	879..7157
Translation	MYGTGTGAQTGVTTPRSSASLRPLTVSHGSLETSFLIPTGLHFHATQLKERFAAILPAPTDELAQDDEPSSIFELVARYMGFIATEVTQGEDDAQGSYEEVLKIILNEFERAFLRGNDVHALVSTMEGIDDKKLEVIRCYYLARSASNRAIKPYESALFRAAGENAAKIFTIFGGQGNIEEYFEELRELHHTYPAFVGELITTSAELLQTLSSHPDAEKMYPKGLDVLGWLQSPEATPDVDYLISAPVSFPLIGLVQLAHYQVTCKVLGVHPGVLRERISGTTGHSQGVVLSAITAAADSWESFEEHSKAALTILFWIGARSQQTFPRTSLAPSMLQDSADNGEGVPTPMLSIRDLPQAEVQKHIDQTNAYLPEDQHISIALINSPRNMVVAGPPSSLHGLNLQLRKLKAATGLDQTRIPHTERKVRFVNRFLPITAPFHSKYLAKASDLIAEDLKDVQIDSRTLGMPVYNTFTGKDLREEVEGNIVPSLIRMITGETVHWEKATVFQDATHILDFGPGGISGLGILTSRNKEGTGVRVILAGTVNGTVTDVGYKPELFDRDEEQAVKFAVNWVKEYGPRLVKNSVGRTYVDTKMSRLLGLPPLLVAGMTPTTVPWDFIAATMNAGYQIELAGGGYYNAKTMTEAISKIEKAVPAGRGICVNLIYVNPRAMGWQIPLLGRLRAEGYPIEGLTIGAGVPSIEVAQEYIETLGLKHISFKPGSGEAIQAVINIAKANPTFPVILQWTGGRGGGHHSFEDFHHPILNLYGRIRRQENIILVAGSGFGGADDTYPYLTGDWSIKYGYPPMPFDGCMFGSRMMVAKEAHTSLAAKQAIVDAPGLDDSDWEKTYKGPAGGVITVRSEMGEPIHKLATRGVRFWAEMDQKIFSLPKEKRVAALKASRDYIIEKLNKDFQKVWFGQNKEGKAVDLEDMTYAEIVRRLVELLYVKDEARWIDQSYTKLTGDFIHRIEERFTTTPGQPSRLQSYADLKEPYSAVEQILSHYPEAEKQLINAQDVQHFLLLCQRRGQKPVTFVPVLDENFEFFFKKDSLWQSEDLAAVVGKDVGRTCILQGPMAAKHATKVDEPIKDILDGIHNGHIALLTRDMYGGDESKIPTIEYFGGKLIESEIPLDIEGLTVSYDEHKNTYRLSSSPSVQLPSLDAWLSLLAGHQRNWRFALLQSDVLVQGQKYQTNPMRRIFAPARGLFVEILHPNNPEKTVILVKEQPRPNRYAEVIEVKLDGKNEIVVNMIKDTTALGKPVPLTLKFIYNPEAGYAPIHEVMDGRNDRIKEFYWRAWFGDEKLDLDSSVHEVFDGGKTTITSEAINEFVHAVGNTGEAFVDRPDKVMYAPMDFAIVVGWKAITKPIFPRTIDGDLLKLVHLSNQFRMMPGAEPLKKGDVVSTTAQINAVINQESGKMVEVCGTITRDGEKVMEVTSQFLYRGTYTDFENTFQRKVETPMQLHLATSKDVAVLRSKDWFMMDDVENPDIELLGQTLTFRLQTLVRFKNKTVFSHVETQGQVLLELPTKEIIQVATVNYYAGESHGNPVIDYLQRHGSSIEQPINFENPIPLSGKTPLQLKAPSSNETYARVSGDYNPIHVSRVFSSYANLPGTITHGMYSSAAVRSLVETWAAENKVGRVRSFHASLTGMVLPNDDINVKLQHVGMVAGRKIIKVEASNKETEEKVLLGEAEIEQPITAYIFTGQGSQEQGMGMDLYNSSPVAKAVWDRADKYLMDTYGFAITNIVRNNPKELTIHFGGPRGKAIRQNYMSMTFETVAADGSIKSERMFKEIDEKTTSYTYRSPNGLLSATQFTQPALTLMEKASFEDMKAKGLVPRDSTFAGHSLGEYSALAALADVMPIESLVSVVFYRGLTMQVAVERDETGRSNYGMCAVNPSRINKTFTEEALRFVVGSIAETTGWLLEIVNFNVENMQYVCAGDLRALDTLGGVCNAIKMMKIDIEKMRKEYSAETVKEQLVEIIKSCANDTQAKPTPLVLQRGFATIPLPGIDVPFHSTFLRSGVKPFRSFLLKKINKTTIDPSKLIGKYIPNVTAKPFAITKEYFEDVYRLTNSPKIQQILANWDKYQEEGVSGIPDAE

Target ORF information:

RefSeq Version	XM_011395933.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A fatty acid synthase beta subunit dehydratase (cel-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395933.1

ORF Insert Sequence:

ATGTACGGTA CCGGTACCGG CGCTCAGACG GGCGTCACCA CCCCCAGGTC GTCTGCCTCT 
CTGCGGCCGC TCACCGTCTC TCACGGGTCC CTCGAAACCT CTTTCTTGAT ACCCACCGGC 
CTTCATTTCC ACGCTACTCA GCTAAAGGAG CGTTTTGCTG CTATCCTTCC CGCCCCTACT 
GACGAGCTCG CGCAAGATGA CGAGCCCTCG TCCATTTTCG AGCTTGTTGC CAGATACATG 
GGCTTTATCG CGACCGAGGT GACACAAGGC GAGGACGACG CTCAGGGTTC ATACGAGGAG 
GTACTCAAAA TCATCCTGAA CGAGTTCGAG AGGGCGTTCC TGCGGGGCAA CGATGTCCAT 
GCCCTGGTCT CTACAATGGA GGGAATCGAC GACAAGAAGC TCGAAGTCAT TCGCTGCTAC 
TACCTCGCGC GATCAGCTAG CAACAGAGCC ATCAAGCCTT ACGAGTCGGC TCTGTTCCGC 
GCCGCTGGTG AAAACGCGGC CAAGATCTTC ACCATCTTTG GTGGCCAGGG AAACATCGAG 
GAGTACTTCG AAGAGCTCCG GGAGCTGCAC CACACCTATC CGGCATTCGT CGGCGAGCTG 
ATCACCACAT CCGCCGAGCT GCTCCAGACC CTGTCCAGCC ACCCTGATGC CGAGAAGATG 
TACCCCAAGG GCCTTGACGT GCTTGGTTGG CTTCAGAGCC CAGAGGCCAC CCCCGACGTC 
GACTACCTTA TCTCTGCCCC CGTCAGCTTC CCCTTGATTG GTTTGGTACA GCTGGCACAC 
TATCAGGTCA CCTGCAAGGT GCTGGGTGTG CATCCTGGCG TCCTTCGTGA GAGAATCAGC 
GGAACGACAG GTCACTCTCA AGGTGTTGTT CTTTCCGCTA TCACTGCGGC CGCGGATTCG 
TGGGAAAGTT TCGAGGAGCA TAGCAAGGCT GCCTTGACCA TCCTCTTCTG GATCGGCGCT 
CGCAGCCAGC AAACCTTCCC TAGAACTTCC CTAGCCCCCT CGATGCTTCA AGACTCGGCT 
GATAATGGCG AGGGTGTTCC TACCCCCATG CTCAGCATTC GAGACCTTCC CCAAGCCGAG 
GTTCAGAAGC ATATCGATCA GACTAATGCC TACCTTCCTG AGGACCAGCA TATTTCCATT 
GCTTTGATCA ACAGCCCCCG GAACATGGTC GTTGCCGGGC CTCCCAGCTC TCTGCACGGT 
CTTAACTTGC AGCTGAGAAA GCTCAAGGCT GCTACTGGTC TTGATCAGAC CAGAATCCCC 
CATACCGAGC GTAAGGTTCG GTTCGTGAAC CGTTTCCTGC CCATCACTGC TCCTTTCCAC 
AGCAAGTACC TCGCCAAGGC TTCCGACCTG ATTGCCGAAG ACCTCAAGGA CGTTCAAATT 
GACTCTCGCA CCCTGGGTAT GCCCGTTTAT AACACTTTCA CGGGCAAGGA TCTTCGGGAG 
GAAGTTGAGG GCAACATTGT TCCTTCCCTT ATCCGCATGA TTACGGGCGA GACCGTCCAC 
TGGGAGAAGG CCACCGTGTT CCAGGATGCC ACCCATATCC TCGATTTTGG CCCTGGCGGT 
ATCTCTGGTC TCGGCATTCT GACTAGCCGT AACAAGGAGG GTACCGGCGT TCGCGTCATT 
CTTGCTGGCA CGGTGAACGG AACTGTCACT GATGTTGGCT ACAAGCCCGA GTTGTTCGAC 
CGTGACGAGG AGCAGGCAGT CAAGTTTGCT GTGAACTGGG TCAAGGAATA CGGTCCTAGA 
CTGGTCAAGA ACTCTGTTGG TCGTACATAC GTTGATACCA AGATGAGCAG ACTTCTTGGC 
CTCCCGCCCT TGTTGGTCGC TGGTATGACT CCTACCACTG TGCCTTGGGA CTTCATTGCC 
GCCACCATGA ATGCTGGCTA CCAGATCGAG CTTGCTGGTG GTGGCTACTA CAATGCCAAG 
ACCATGACCG AGGCCATCTC CAAGATTGAG AAGGCTGTTC CTGCTGGTCG CGGTATCTGC 
GTCAACTTGA TCTATGTCAA CCCTCGCGCC ATGGGCTGGC AGATTCCCCT GCTTGGCAGG 
CTCAGAGCTG AGGGCTATCC CATCGAAGGT CTCACCATCG GTGCTGGCGT TCCTTCCATC 
GAGGTCGCGC AGGAGTATAT CGAAACTCTG GGACTCAAGC ACATCTCTTT CAAGCCTGGT 
TCGGGTGAGG CTATTCAGGC CGTCATCAAC ATCGCCAAGG CCAACCCTAC CTTCCCCGTG 
ATTCTCCAAT GGACCGGTGG CAGAGGTGGT GGTCATCACT CGTTTGAGGA TTTCCATCAC 
CCCATCCTCA ACCTCTATGG CCGCATCCGC CGCCAAGAAA ACATCATTCT CGTTGCCGGT 
AGCGGTTTCG GAGGTGCTGA TGACACTTAC CCCTACCTCA CTGGTGACTG GTCCATCAAG 
TATGGCTATC CCCCCATGCC CTTCGACGGC TGCATGTTCG GTAGTCGTAT GATGGTCGCC 
AAGGAAGCCC ACACCAGCCT GGCTGCCAAG CAGGCTATCG TTGACGCTCC TGGTCTTGAC 
GACAGTGACT GGGAGAAGAC CTACAAGGGC CCAGCTGGCG GTGTCATCAC TGTCCGCTCT 
GAGATGGGTG AGCCCATTCA TAAGCTCGCC ACTCGTGGTG TCCGCTTCTG GGCTGAGATG 
GACCAGAAGA TCTTCAGCCT TCCCAAGGAG AAGAGGGTCG CTGCTCTTAA GGCTAGCCGT 
GATTACATCA TCGAGAAGCT CAACAAGGAC TTCCAAAAGG TCTGGTTTGG TCAGAACAAG 
GAGGGCAAGG CCGTTGATCT TGAGGACATG ACATATGCTG AGATTGTTCG CCGCCTGGTC 
GAGCTTCTCT ACGTCAAGGA TGAGGCTAGG TGGATTGATC AGTCGTACAC CAAGCTTACT 
GGTGACTTTA TCCACCGTAT TGAGGAGCGA TTTACTACCA CGCCTGGCCA GCCTTCCAGA 
CTCCAGAGTT ATGCTGACCT CAAGGAGCCC TACTCTGCTG TTGAGCAGAT TCTCTCGCAC 
TATCCCGAGG CCGAGAAGCA ACTCATCAAC GCTCAGGATG TCCAACATTT CCTCCTGCTC 
TGTCAACGTC GTGGACAGAA GCCCGTCACC TTTGTGCCTG TTCTCGATGA GAACTTCGAG 
TTTTTCTTCA AGAAGGATTC GCTCTGGCAG TCGGAGGATC TTGCGGCTGT CGTTGGCAAG 
GATGTCGGCC GTACCTGTAT TCTTCAGGGT CCCATGGCTG CCAAGCACGC CACTAAGGTG 
GACGAGCCCA TCAAGGACAT CCTCGACGGA ATTCACAACG GTCACATCGC CTTGCTCACC 
CGTGATATGT ATGGCGGCGA TGAGAGCAAG ATCCCTACCA TTGAGTACTT TGGTGGTAAG 
CTGATCGAGT CAGAAATTCC TCTCGATATC GAGGGTCTTA CTGTCTCCTA TGATGAGCAC 
AAGAACACCT ATCGTCTGTC CTCATCGCCT TCCGTTCAAC TTCCTTCGCT GGACGCCTGG 
CTTTCGCTTC TCGCCGGACA CCAAAGAAAC TGGCGTTTCG CCCTTCTCCA GTCCGACGTC 
CTCGTCCAAG GACAAAAATA CCAGACCAAC CCCATGCGCC GCATCTTTGC GCCGGCTCGT 
GGTCTGTTTG TCGAGATTCT ACACCCGAAC AACCCCGAGA AGACGGTCAT TCTCGTCAAG 
GAGCAGCCAC GCCCCAACCG CTACGCTGAG GTCATTGAGG TCAAGCTGGA CGGCAAGAAC 
GAGATTGTAG TCAACATGAT CAAGGATACC ACGGCCCTCG GAAAGCCAGT GCCCCTCACT 
CTCAAGTTTA TCTACAACCC CGAGGCTGGC TATGCTCCTA TTCATGAGGT TATGGATGGT 
CGCAATGATC GTATCAAGGA GTTCTACTGG CGCGCGTGGT TCGGCGATGA GAAGCTCGAC 
CTCGATTCCT CCGTTCATGA AGTCTTCGAC GGCGGCAAGA CCACCATCAC CAGCGAGGCC 
ATCAATGAGT TTGTTCACGC CGTTGGCAAC ACCGGAGAGG CCTTCGTTGA CAGACCCGAC 
AAGGTCATGT ATGCTCCCAT GGACTTTGCT ATCGTTGTTG GCTGGAAGGC TATCACCAAG 
CCTATTTTCC CTCGCACCAT TGACGGTGAC CTGCTCAAGC TCGTCCATTT GTCCAACCAG 
TTCCGCATGA TGCCTGGTGC TGAGCCCCTC AAGAAGGGTG ATGTGGTTTC GACTACTGCT 
CAGATCAACG CTGTCATCAA CCAGGAGTCT GGAAAGATGG TCGAGGTCTG TGGTACCATC 
ACTCGTGACG GTGAGAAGGT CATGGAGGTT ACTTCGCAGT TCTTGTATCG TGGTACCTAC 
ACCGACTTCG AGAACACATT CCAGCGCAAG GTGGAGACCC CTATGCAGCT TCATCTTGCC 
ACCTCCAAGG ACGTGGCCGT CCTTCGCTCC AAGGATTGGT TCATGATGGA TGATGTTGAA 
AACCCTGATA TTGAGCTTCT CGGCCAGACC CTCACCTTCC GTCTCCAGAC CTTGGTTCGC 
TTCAAGAACA AGACTGTCTT CAGCCACGTG GAGACCCAGG GCCAGGTTCT TCTTGAGCTC 
CCGACCAAGG AGATTATCCA GGTTGCCACT GTCAACTACT ACGCTGGAGA ATCTCACGGA 
AATCCCGTCA TCGACTACCT CCAGCGTCAT GGTTCCTCCA TTGAACAGCC GATCAACTTT 
GAGAACCCTA TCCCTCTGAG CGGAAAGACC CCCCTCCAAC TCAAGGCGCC CTCGTCCAAC 
GAGACTTATG CCCGCGTCTC CGGTGATTAT AACCCCATTC ACGTTTCTCG TGTCTTTTCC 
AGCTATGCCA ACCTTCCTGG CACCATCACT CACGGCATGT ACTCGAGTGC CGCGGTGCGC 
AGCTTGGTTG AGACCTGGGC TGCCGAGAAC AAGGTTGGCC GCGTTCGCAG CTTCCACGCC 
TCTCTTACCG GCATGGTTCT GCCCAATGAT GATATCAACG TGAAGTTGCA GCACGTCGGC 
ATGGTTGCTG GCCGGAAGAT TATCAAGGTT GAGGCTAGCA ACAAGGAGAC CGAGGAAAAG 
GTGCTCCTGG GCGAGGCCGA AATTGAGCAG CCTATCACCG CTTACATCTT CACCGGCCAG 
GGCTCGCAGG AGCAGGGCAT GGGTATGGAT CTTTATAACA GCAGCCCTGT GGCCAAGGCT 
GTCTGGGATC GGGCCGACAA GTATCTCATG GACACTTATG GCTTTGCCAT CACCAACATT 
GTCCGGAACA ACCCCAAGGA GCTCACTATT CACTTTGGTG GCCCTCGTGG CAAGGCCATT 
CGCCAGAACT ACATGTCCAT GACTTTCGAG ACTGTGGCTG CCGATGGCTC GATCAAGTCG 
GAGCGCATGT TCAAGGAGAT CGACGAGAAG ACTACCTCTT ACACTTACCG CTCACCCAAC 
GGTCTTCTCT CGGCTACCCA GTTCACGCAG CCCGCTCTTA CTCTAATGGA AAAGGCCAGC 
TTCGAGGACA TGAAGGCCAA GGGACTTGTG CCCCGTGATA GCACCTTCGC TGGTCACTCT 
CTTGGCGAAT ACTCTGCGCT GGCTGCCCTT GCCGATGTCA TGCCCATCGA GTCCCTCGTG 
TCCGTCGTCT TCTACCGTGG TCTCACCATG CAAGTCGCTG TCGAGCGCGA TGAGACAGGC 
CGCTCCAACT ATGGCATGTG CGCTGTCAAC CCCAGCCGTA TCAACAAGAC TTTCACCGAA 
GAGGCCTTGC GGTTCGTGGT CGGTAGCATC GCAGAGACCA CCGGATGGCT TCTTGAAATC 
GTCAACTTCA ACGTCGAGAA CATGCAGTAC GTCTGTGCTG GCGACTTGCG TGCGCTCGAC 
ACCCTTGGCG GTGTGTGCAA CGCTATCAAG ATGATGAAGA TCGACATTGA GAAAATGCGC 
AAGGAGTACT CTGCCGAGAC TGTCAAGGAG CAGCTGGTCG AGATCATCAA GTCGTGCGCC 
AACGACACTC AGGCTAAGCC CACTCCCCTC GTTCTCCAGC GTGGCTTTGC AACTATCCCG 
CTCCCCGGTA TCGATGTGCC CTTCCACTCG ACATTCCTGC GCTCTGGTGT CAAGCCTTTC 
AGAAGCTTCT TGCTCAAGAA GATCAACAAG ACTACCATCG ACCCATCGAA GCTTATTGGC 
AAGTACATTC CCAACGTGAC TGCTAAGCCA TTCGCAATCA CCAAGGAGTA TTTCGAGGAT 
GTCTACCGGT TGACGAACTC CCCTAAGATC CAACAGATCT TGGCAAACTG GGATAAGTAC 
CAGGAGGAGG GTGTCAGTGG AATCCCTGAT GCGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cel-2 ( XM_011395934.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394236.1 Neurospora crassa OR74A fatty acid synthase beta subunit dehydratase, variant (cel-2), mRNA.

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CloneID	ONf01152
Clone ID Related Accession (Same CDS sequence)	XM_011395933.1 , XM_011395934.1
Accession Version	XM_011395934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	fatty acid synthase beta subunit dehydratase, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504).

ATGTACGGTA CCGGTACCGG CGCTCAGACG GGCGTCACCA CCCCCAGGTC GTCTGCCTCT 
CTGCGGCCGC TCACCGTCTC TCACGGGTCC CTCGAAACCT CTTTCTTGAT ACCCACCGGC 
CTTCATTTCC ACGCTACTCA GCTAAAGGAG CGTTTTGCTG CTATCCTTCC CGCCCCTACT 
GACGAGCTCG CGCAAGATGA CGAGCCCTCG TCCATTTTCG AGCTTGTTGC CAGATACATG 
GGCTTTATCG CGACCGAGGT GACACAAGGC GAGGACGACG CTCAGGGTTC ATACGAGGAG 
GTACTCAAAA TCATCCTGAA CGAGTTCGAG AGGGCGTTCC TGCGGGGCAA CGATGTCCAT 
GCCCTGGTCT CTACAATGGA GGGAATCGAC GACAAGAAGC TCGAAGTCAT TCGCTGCTAC 
TACCTCGCGC GATCAGCTAG CAACAGAGCC ATCAAGCCTT ACGAGTCGGC TCTGTTCCGC 
GCCGCTGGTG AAAACGCGGC CAAGATCTTC ACCATCTTTG GTGGCCAGGG AAACATCGAG 
GAGTACTTCG AAGAGCTCCG GGAGCTGCAC CACACCTATC CGGCATTCGT CGGCGAGCTG 
ATCACCACAT CCGCCGAGCT GCTCCAGACC CTGTCCAGCC ACCCTGATGC CGAGAAGATG 
TACCCCAAGG GCCTTGACGT GCTTGGTTGG CTTCAGAGCC CAGAGGCCAC CCCCGACGTC 
GACTACCTTA TCTCTGCCCC CGTCAGCTTC CCCTTGATTG GTTTGGTACA GCTGGCACAC 
TATCAGGTCA CCTGCAAGGT GCTGGGTGTG CATCCTGGCG TCCTTCGTGA GAGAATCAGC 
GGAACGACAG GTCACTCTCA AGGTGTTGTT CTTTCCGCTA TCACTGCGGC CGCGGATTCG 
TGGGAAAGTT TCGAGGAGCA TAGCAAGGCT GCCTTGACCA TCCTCTTCTG GATCGGCGCT 
CGCAGCCAGC AAACCTTCCC TAGAACTTCC CTAGCCCCCT CGATGCTTCA AGACTCGGCT 
GATAATGGCG AGGGTGTTCC TACCCCCATG CTCAGCATTC GAGACCTTCC CCAAGCCGAG 
GTTCAGAAGC ATATCGATCA GACTAATGCC TACCTTCCTG AGGACCAGCA TATTTCCATT 
GCTTTGATCA ACAGCCCCCG GAACATGGTC GTTGCCGGGC CTCCCAGCTC TCTGCACGGT 
CTTAACTTGC AGCTGAGAAA GCTCAAGGCT GCTACTGGTC TTGATCAGAC CAGAATCCCC 
CATACCGAGC GTAAGGTTCG GTTCGTGAAC CGTTTCCTGC CCATCACTGC TCCTTTCCAC 
AGCAAGTACC TCGCCAAGGC TTCCGACCTG ATTGCCGAAG ACCTCAAGGA CGTTCAAATT 
GACTCTCGCA CCCTGGGTAT GCCCGTTTAT AACACTTTCA CGGGCAAGGA TCTTCGGGAG 
GAAGTTGAGG GCAACATTGT TCCTTCCCTT ATCCGCATGA TTACGGGCGA GACCGTCCAC 
TGGGAGAAGG CCACCGTGTT CCAGGATGCC ACCCATATCC TCGATTTTGG CCCTGGCGGT 
ATCTCTGGTC TCGGCATTCT GACTAGCCGT AACAAGGAGG GTACCGGCGT TCGCGTCATT 
CTTGCTGGCA CGGTGAACGG AACTGTCACT GATGTTGGCT ACAAGCCCGA GTTGTTCGAC 
CGTGACGAGG AGCAGGCAGT CAAGTTTGCT GTGAACTGGG TCAAGGAATA CGGTCCTAGA 
CTGGTCAAGA ACTCTGTTGG TCGTACATAC GTTGATACCA AGATGAGCAG ACTTCTTGGC 
CTCCCGCCCT TGTTGGTCGC TGGTATGACT CCTACCACTG TGCCTTGGGA CTTCATTGCC 
GCCACCATGA ATGCTGGCTA CCAGATCGAG CTTGCTGGTG GTGGCTACTA CAATGCCAAG 
ACCATGACCG AGGCCATCTC CAAGATTGAG AAGGCTGTTC CTGCTGGTCG CGGTATCTGC 
GTCAACTTGA TCTATGTCAA CCCTCGCGCC ATGGGCTGGC AGATTCCCCT GCTTGGCAGG 
CTCAGAGCTG AGGGCTATCC CATCGAAGGT CTCACCATCG GTGCTGGCGT TCCTTCCATC 
GAGGTCGCGC AGGAGTATAT CGAAACTCTG GGACTCAAGC ACATCTCTTT CAAGCCTGGT 
TCGGGTGAGG CTATTCAGGC CGTCATCAAC ATCGCCAAGG CCAACCCTAC CTTCCCCGTG 
ATTCTCCAAT GGACCGGTGG CAGAGGTGGT GGTCATCACT CGTTTGAGGA TTTCCATCAC 
CCCATCCTCA ACCTCTATGG CCGCATCCGC CGCCAAGAAA ACATCATTCT CGTTGCCGGT 
AGCGGTTTCG GAGGTGCTGA TGACACTTAC CCCTACCTCA CTGGTGACTG GTCCATCAAG 
TATGGCTATC CCCCCATGCC CTTCGACGGC TGCATGTTCG GTAGTCGTAT GATGGTCGCC 
AAGGAAGCCC ACACCAGCCT GGCTGCCAAG CAGGCTATCG TTGACGCTCC TGGTCTTGAC 
GACAGTGACT GGGAGAAGAC CTACAAGGGC CCAGCTGGCG GTGTCATCAC TGTCCGCTCT 
GAGATGGGTG AGCCCATTCA TAAGCTCGCC ACTCGTGGTG TCCGCTTCTG GGCTGAGATG 
GACCAGAAGA TCTTCAGCCT TCCCAAGGAG AAGAGGGTCG CTGCTCTTAA GGCTAGCCGT 
GATTACATCA TCGAGAAGCT CAACAAGGAC TTCCAAAAGG TCTGGTTTGG TCAGAACAAG 
GAGGGCAAGG CCGTTGATCT TGAGGACATG ACATATGCTG AGATTGTTCG CCGCCTGGTC 
GAGCTTCTCT ACGTCAAGGA TGAGGCTAGG TGGATTGATC AGTCGTACAC CAAGCTTACT 
GGTGACTTTA TCCACCGTAT TGAGGAGCGA TTTACTACCA CGCCTGGCCA GCCTTCCAGA 
CTCCAGAGTT ATGCTGACCT CAAGGAGCCC TACTCTGCTG TTGAGCAGAT TCTCTCGCAC 
TATCCCGAGG CCGAGAAGCA ACTCATCAAC GCTCAGGATG TCCAACATTT CCTCCTGCTC 
TGTCAACGTC GTGGACAGAA GCCCGTCACC TTTGTGCCTG TTCTCGATGA GAACTTCGAG 
TTTTTCTTCA AGAAGGATTC GCTCTGGCAG TCGGAGGATC TTGCGGCTGT CGTTGGCAAG 
GATGTCGGCC GTACCTGTAT TCTTCAGGGT CCCATGGCTG CCAAGCACGC CACTAAGGTG 
GACGAGCCCA TCAAGGACAT CCTCGACGGA ATTCACAACG GTCACATCGC CTTGCTCACC 
CGTGATATGT ATGGCGGCGA TGAGAGCAAG ATCCCTACCA TTGAGTACTT TGGTGGTAAG 
CTGATCGAGT CAGAAATTCC TCTCGATATC GAGGGTCTTA CTGTCTCCTA TGATGAGCAC 
AAGAACACCT ATCGTCTGTC CTCATCGCCT TCCGTTCAAC TTCCTTCGCT GGACGCCTGG 
CTTTCGCTTC TCGCCGGACA CCAAAGAAAC TGGCGTTTCG CCCTTCTCCA GTCCGACGTC 
CTCGTCCAAG GACAAAAATA CCAGACCAAC CCCATGCGCC GCATCTTTGC GCCGGCTCGT 
GGTCTGTTTG TCGAGATTCT ACACCCGAAC AACCCCGAGA AGACGGTCAT TCTCGTCAAG 
GAGCAGCCAC GCCCCAACCG CTACGCTGAG GTCATTGAGG TCAAGCTGGA CGGCAAGAAC 
GAGATTGTAG TCAACATGAT CAAGGATACC ACGGCCCTCG GAAAGCCAGT GCCCCTCACT 
CTCAAGTTTA TCTACAACCC CGAGGCTGGC TATGCTCCTA TTCATGAGGT TATGGATGGT 
CGCAATGATC GTATCAAGGA GTTCTACTGG CGCGCGTGGT TCGGCGATGA GAAGCTCGAC 
CTCGATTCCT CCGTTCATGA AGTCTTCGAC GGCGGCAAGA CCACCATCAC CAGCGAGGCC 
ATCAATGAGT TTGTTCACGC CGTTGGCAAC ACCGGAGAGG CCTTCGTTGA CAGACCCGAC 
AAGGTCATGT ATGCTCCCAT GGACTTTGCT ATCGTTGTTG GCTGGAAGGC TATCACCAAG 
CCTATTTTCC CTCGCACCAT TGACGGTGAC CTGCTCAAGC TCGTCCATTT GTCCAACCAG 
TTCCGCATGA TGCCTGGTGC TGAGCCCCTC AAGAAGGGTG ATGTGGTTTC GACTACTGCT 
CAGATCAACG CTGTCATCAA CCAGGAGTCT GGAAAGATGG TCGAGGTCTG TGGTACCATC 
ACTCGTGACG GTGAGAAGGT CATGGAGGTT ACTTCGCAGT TCTTGTATCG TGGTACCTAC 
ACCGACTTCG AGAACACATT CCAGCGCAAG GTGGAGACCC CTATGCAGCT TCATCTTGCC 
ACCTCCAAGG ACGTGGCCGT CCTTCGCTCC AAGGATTGGT TCATGATGGA TGATGTTGAA 
AACCCTGATA TTGAGCTTCT CGGCCAGACC CTCACCTTCC GTCTCCAGAC CTTGGTTCGC 
TTCAAGAACA AGACTGTCTT CAGCCACGTG GAGACCCAGG GCCAGGTTCT TCTTGAGCTC 
CCGACCAAGG AGATTATCCA GGTTGCCACT GTCAACTACT ACGCTGGAGA ATCTCACGGA 
AATCCCGTCA TCGACTACCT CCAGCGTCAT GGTTCCTCCA TTGAACAGCC GATCAACTTT 
GAGAACCCTA TCCCTCTGAG CGGAAAGACC CCCCTCCAAC TCAAGGCGCC CTCGTCCAAC 
GAGACTTATG CCCGCGTCTC CGGTGATTAT AACCCCATTC ACGTTTCTCG TGTCTTTTCC 
AGCTATGCCA ACCTTCCTGG CACCATCACT CACGGCATGT ACTCGAGTGC CGCGGTGCGC 
AGCTTGGTTG AGACCTGGGC TGCCGAGAAC AAGGTTGGCC GCGTTCGCAG CTTCCACGCC 
TCTCTTACCG GCATGGTTCT GCCCAATGAT GATATCAACG TGAAGTTGCA GCACGTCGGC 
ATGGTTGCTG GCCGGAAGAT TATCAAGGTT GAGGCTAGCA ACAAGGAGAC CGAGGAAAAG 
GTGCTCCTGG GCGAGGCCGA AATTGAGCAG CCTATCACCG CTTACATCTT CACCGGCCAG 
GGCTCGCAGG AGCAGGGCAT GGGTATGGAT CTTTATAACA GCAGCCCTGT GGCCAAGGCT 
GTCTGGGATC GGGCCGACAA GTATCTCATG GACACTTATG GCTTTGCCAT CACCAACATT 
GTCCGGAACA ACCCCAAGGA GCTCACTATT CACTTTGGTG GCCCTCGTGG CAAGGCCATT 
CGCCAGAACT ACATGTCCAT GACTTTCGAG ACTGTGGCTG CCGATGGCTC GATCAAGTCG 
GAGCGCATGT TCAAGGAGAT CGACGAGAAG ACTACCTCTT ACACTTACCG CTCACCCAAC 
GGTCTTCTCT CGGCTACCCA GTTCACGCAG CCCGCTCTTA CTCTAATGGA AAAGGCCAGC 
TTCGAGGACA TGAAGGCCAA GGGACTTGTG CCCCGTGATA GCACCTTCGC TGGTCACTCT 
CTTGGCGAAT ACTCTGCGCT GGCTGCCCTT GCCGATGTCA TGCCCATCGA GTCCCTCGTG 
TCCGTCGTCT TCTACCGTGG TCTCACCATG CAAGTCGCTG TCGAGCGCGA TGAGACAGGC 
CGCTCCAACT ATGGCATGTG CGCTGTCAAC CCCAGCCGTA TCAACAAGAC TTTCACCGAA 
GAGGCCTTGC GGTTCGTGGT CGGTAGCATC GCAGAGACCA CCGGATGGCT TCTTGAAATC 
GTCAACTTCA ACGTCGAGAA CATGCAGTAC GTCTGTGCTG GCGACTTGCG TGCGCTCGAC 
ACCCTTGGCG GTGTGTGCAA CGCTATCAAG ATGATGAAGA TCGACATTGA GAAAATGCGC 
AAGGAGTACT CTGCCGAGAC TGTCAAGGAG CAGCTGGTCG AGATCATCAA GTCGTGCGCC 
AACGACACTC AGGCTAAGCC CACTCCCCTC GTTCTCCAGC GTGGCTTTGC AACTATCCCG 
CTCCCCGGTA TCGATGTGCC CTTCCACTCG ACATTCCTGC GCTCTGGTGT CAAGCCTTTC 
AGAAGCTTCT TGCTCAAGAA GATCAACAAG ACTACCATCG ACCCATCGAA GCTTATTGGC 
AAGTACATTC CCAACGTGAC TGCTAAGCCA TTCGCAATCA CCAAGGAGTA TTTCGAGGAT 
GTCTACCGGT TGACGAACTC CCCTAAGATC CAACAGATCT TGGCAAACTG GGATAAGTAC 
CAGGAGGAGG GTGTCAGTGG AATCCCTGAT GCGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394236.1
CDS	550..6828
Translation	MYGTGTGAQTGVTTPRSSASLRPLTVSHGSLETSFLIPTGLHFHATQLKERFAAILPAPTDELAQDDEPSSIFELVARYMGFIATEVTQGEDDAQGSYEEVLKIILNEFERAFLRGNDVHALVSTMEGIDDKKLEVIRCYYLARSASNRAIKPYESALFRAAGENAAKIFTIFGGQGNIEEYFEELRELHHTYPAFVGELITTSAELLQTLSSHPDAEKMYPKGLDVLGWLQSPEATPDVDYLISAPVSFPLIGLVQLAHYQVTCKVLGVHPGVLRERISGTTGHSQGVVLSAITAAADSWESFEEHSKAALTILFWIGARSQQTFPRTSLAPSMLQDSADNGEGVPTPMLSIRDLPQAEVQKHIDQTNAYLPEDQHISIALINSPRNMVVAGPPSSLHGLNLQLRKLKAATGLDQTRIPHTERKVRFVNRFLPITAPFHSKYLAKASDLIAEDLKDVQIDSRTLGMPVYNTFTGKDLREEVEGNIVPSLIRMITGETVHWEKATVFQDATHILDFGPGGISGLGILTSRNKEGTGVRVILAGTVNGTVTDVGYKPELFDRDEEQAVKFAVNWVKEYGPRLVKNSVGRTYVDTKMSRLLGLPPLLVAGMTPTTVPWDFIAATMNAGYQIELAGGGYYNAKTMTEAISKIEKAVPAGRGICVNLIYVNPRAMGWQIPLLGRLRAEGYPIEGLTIGAGVPSIEVAQEYIETLGLKHISFKPGSGEAIQAVINIAKANPTFPVILQWTGGRGGGHHSFEDFHHPILNLYGRIRRQENIILVAGSGFGGADDTYPYLTGDWSIKYGYPPMPFDGCMFGSRMMVAKEAHTSLAAKQAIVDAPGLDDSDWEKTYKGPAGGVITVRSEMGEPIHKLATRGVRFWAEMDQKIFSLPKEKRVAALKASRDYIIEKLNKDFQKVWFGQNKEGKAVDLEDMTYAEIVRRLVELLYVKDEARWIDQSYTKLTGDFIHRIEERFTTTPGQPSRLQSYADLKEPYSAVEQILSHYPEAEKQLINAQDVQHFLLLCQRRGQKPVTFVPVLDENFEFFFKKDSLWQSEDLAAVVGKDVGRTCILQGPMAAKHATKVDEPIKDILDGIHNGHIALLTRDMYGGDESKIPTIEYFGGKLIESEIPLDIEGLTVSYDEHKNTYRLSSSPSVQLPSLDAWLSLLAGHQRNWRFALLQSDVLVQGQKYQTNPMRRIFAPARGLFVEILHPNNPEKTVILVKEQPRPNRYAEVIEVKLDGKNEIVVNMIKDTTALGKPVPLTLKFIYNPEAGYAPIHEVMDGRNDRIKEFYWRAWFGDEKLDLDSSVHEVFDGGKTTITSEAINEFVHAVGNTGEAFVDRPDKVMYAPMDFAIVVGWKAITKPIFPRTIDGDLLKLVHLSNQFRMMPGAEPLKKGDVVSTTAQINAVINQESGKMVEVCGTITRDGEKVMEVTSQFLYRGTYTDFENTFQRKVETPMQLHLATSKDVAVLRSKDWFMMDDVENPDIELLGQTLTFRLQTLVRFKNKTVFSHVETQGQVLLELPTKEIIQVATVNYYAGESHGNPVIDYLQRHGSSIEQPINFENPIPLSGKTPLQLKAPSSNETYARVSGDYNPIHVSRVFSSYANLPGTITHGMYSSAAVRSLVETWAAENKVGRVRSFHASLTGMVLPNDDINVKLQHVGMVAGRKIIKVEASNKETEEKVLLGEAEIEQPITAYIFTGQGSQEQGMGMDLYNSSPVAKAVWDRADKYLMDTYGFAITNIVRNNPKELTIHFGGPRGKAIRQNYMSMTFETVAADGSIKSERMFKEIDEKTTSYTYRSPNGLLSATQFTQPALTLMEKASFEDMKAKGLVPRDSTFAGHSLGEYSALAALADVMPIESLVSVVFYRGLTMQVAVERDETGRSNYGMCAVNPSRINKTFTEEALRFVVGSIAETTGWLLEIVNFNVENMQYVCAGDLRALDTLGGVCNAIKMMKIDIEKMRKEYSAETVKEQLVEIIKSCANDTQAKPTPLVLQRGFATIPLPGIDVPFHSTFLRSGVKPFRSFLLKKINKTTIDPSKLIGKYIPNVTAKPFAITKEYFEDVYRLTNSPKIQQILANWDKYQEEGVSGIPDAE

Target ORF information:

RefSeq Version	XM_011395934.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A fatty acid synthase beta subunit dehydratase, variant (cel-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395934.1

ORF Insert Sequence:

ATGTACGGTA CCGGTACCGG CGCTCAGACG GGCGTCACCA CCCCCAGGTC GTCTGCCTCT 
CTGCGGCCGC TCACCGTCTC TCACGGGTCC CTCGAAACCT CTTTCTTGAT ACCCACCGGC 
CTTCATTTCC ACGCTACTCA GCTAAAGGAG CGTTTTGCTG CTATCCTTCC CGCCCCTACT 
GACGAGCTCG CGCAAGATGA CGAGCCCTCG TCCATTTTCG AGCTTGTTGC CAGATACATG 
GGCTTTATCG CGACCGAGGT GACACAAGGC GAGGACGACG CTCAGGGTTC ATACGAGGAG 
GTACTCAAAA TCATCCTGAA CGAGTTCGAG AGGGCGTTCC TGCGGGGCAA CGATGTCCAT 
GCCCTGGTCT CTACAATGGA GGGAATCGAC GACAAGAAGC TCGAAGTCAT TCGCTGCTAC 
TACCTCGCGC GATCAGCTAG CAACAGAGCC ATCAAGCCTT ACGAGTCGGC TCTGTTCCGC 
GCCGCTGGTG AAAACGCGGC CAAGATCTTC ACCATCTTTG GTGGCCAGGG AAACATCGAG 
GAGTACTTCG AAGAGCTCCG GGAGCTGCAC CACACCTATC CGGCATTCGT CGGCGAGCTG 
ATCACCACAT CCGCCGAGCT GCTCCAGACC CTGTCCAGCC ACCCTGATGC CGAGAAGATG 
TACCCCAAGG GCCTTGACGT GCTTGGTTGG CTTCAGAGCC CAGAGGCCAC CCCCGACGTC 
GACTACCTTA TCTCTGCCCC CGTCAGCTTC CCCTTGATTG GTTTGGTACA GCTGGCACAC 
TATCAGGTCA CCTGCAAGGT GCTGGGTGTG CATCCTGGCG TCCTTCGTGA GAGAATCAGC 
GGAACGACAG GTCACTCTCA AGGTGTTGTT CTTTCCGCTA TCACTGCGGC CGCGGATTCG 
TGGGAAAGTT TCGAGGAGCA TAGCAAGGCT GCCTTGACCA TCCTCTTCTG GATCGGCGCT 
CGCAGCCAGC AAACCTTCCC TAGAACTTCC CTAGCCCCCT CGATGCTTCA AGACTCGGCT 
GATAATGGCG AGGGTGTTCC TACCCCCATG CTCAGCATTC GAGACCTTCC CCAAGCCGAG 
GTTCAGAAGC ATATCGATCA GACTAATGCC TACCTTCCTG AGGACCAGCA TATTTCCATT 
GCTTTGATCA ACAGCCCCCG GAACATGGTC GTTGCCGGGC CTCCCAGCTC TCTGCACGGT 
CTTAACTTGC AGCTGAGAAA GCTCAAGGCT GCTACTGGTC TTGATCAGAC CAGAATCCCC 
CATACCGAGC GTAAGGTTCG GTTCGTGAAC CGTTTCCTGC CCATCACTGC TCCTTTCCAC 
AGCAAGTACC TCGCCAAGGC TTCCGACCTG ATTGCCGAAG ACCTCAAGGA CGTTCAAATT 
GACTCTCGCA CCCTGGGTAT GCCCGTTTAT AACACTTTCA CGGGCAAGGA TCTTCGGGAG 
GAAGTTGAGG GCAACATTGT TCCTTCCCTT ATCCGCATGA TTACGGGCGA GACCGTCCAC 
TGGGAGAAGG CCACCGTGTT CCAGGATGCC ACCCATATCC TCGATTTTGG CCCTGGCGGT 
ATCTCTGGTC TCGGCATTCT GACTAGCCGT AACAAGGAGG GTACCGGCGT TCGCGTCATT 
CTTGCTGGCA CGGTGAACGG AACTGTCACT GATGTTGGCT ACAAGCCCGA GTTGTTCGAC 
CGTGACGAGG AGCAGGCAGT CAAGTTTGCT GTGAACTGGG TCAAGGAATA CGGTCCTAGA 
CTGGTCAAGA ACTCTGTTGG TCGTACATAC GTTGATACCA AGATGAGCAG ACTTCTTGGC 
CTCCCGCCCT TGTTGGTCGC TGGTATGACT CCTACCACTG TGCCTTGGGA CTTCATTGCC 
GCCACCATGA ATGCTGGCTA CCAGATCGAG CTTGCTGGTG GTGGCTACTA CAATGCCAAG 
ACCATGACCG AGGCCATCTC CAAGATTGAG AAGGCTGTTC CTGCTGGTCG CGGTATCTGC 
GTCAACTTGA TCTATGTCAA CCCTCGCGCC ATGGGCTGGC AGATTCCCCT GCTTGGCAGG 
CTCAGAGCTG AGGGCTATCC CATCGAAGGT CTCACCATCG GTGCTGGCGT TCCTTCCATC 
GAGGTCGCGC AGGAGTATAT CGAAACTCTG GGACTCAAGC ACATCTCTTT CAAGCCTGGT 
TCGGGTGAGG CTATTCAGGC CGTCATCAAC ATCGCCAAGG CCAACCCTAC CTTCCCCGTG 
ATTCTCCAAT GGACCGGTGG CAGAGGTGGT GGTCATCACT CGTTTGAGGA TTTCCATCAC 
CCCATCCTCA ACCTCTATGG CCGCATCCGC CGCCAAGAAA ACATCATTCT CGTTGCCGGT 
AGCGGTTTCG GAGGTGCTGA TGACACTTAC CCCTACCTCA CTGGTGACTG GTCCATCAAG 
TATGGCTATC CCCCCATGCC CTTCGACGGC TGCATGTTCG GTAGTCGTAT GATGGTCGCC 
AAGGAAGCCC ACACCAGCCT GGCTGCCAAG CAGGCTATCG TTGACGCTCC TGGTCTTGAC 
GACAGTGACT GGGAGAAGAC CTACAAGGGC CCAGCTGGCG GTGTCATCAC TGTCCGCTCT 
GAGATGGGTG AGCCCATTCA TAAGCTCGCC ACTCGTGGTG TCCGCTTCTG GGCTGAGATG 
GACCAGAAGA TCTTCAGCCT TCCCAAGGAG AAGAGGGTCG CTGCTCTTAA GGCTAGCCGT 
GATTACATCA TCGAGAAGCT CAACAAGGAC TTCCAAAAGG TCTGGTTTGG TCAGAACAAG 
GAGGGCAAGG CCGTTGATCT TGAGGACATG ACATATGCTG AGATTGTTCG CCGCCTGGTC 
GAGCTTCTCT ACGTCAAGGA TGAGGCTAGG TGGATTGATC AGTCGTACAC CAAGCTTACT 
GGTGACTTTA TCCACCGTAT TGAGGAGCGA TTTACTACCA CGCCTGGCCA GCCTTCCAGA 
CTCCAGAGTT ATGCTGACCT CAAGGAGCCC TACTCTGCTG TTGAGCAGAT TCTCTCGCAC 
TATCCCGAGG CCGAGAAGCA ACTCATCAAC GCTCAGGATG TCCAACATTT CCTCCTGCTC 
TGTCAACGTC GTGGACAGAA GCCCGTCACC TTTGTGCCTG TTCTCGATGA GAACTTCGAG 
TTTTTCTTCA AGAAGGATTC GCTCTGGCAG TCGGAGGATC TTGCGGCTGT CGTTGGCAAG 
GATGTCGGCC GTACCTGTAT TCTTCAGGGT CCCATGGCTG CCAAGCACGC CACTAAGGTG 
GACGAGCCCA TCAAGGACAT CCTCGACGGA ATTCACAACG GTCACATCGC CTTGCTCACC 
CGTGATATGT ATGGCGGCGA TGAGAGCAAG ATCCCTACCA TTGAGTACTT TGGTGGTAAG 
CTGATCGAGT CAGAAATTCC TCTCGATATC GAGGGTCTTA CTGTCTCCTA TGATGAGCAC 
AAGAACACCT ATCGTCTGTC CTCATCGCCT TCCGTTCAAC TTCCTTCGCT GGACGCCTGG 
CTTTCGCTTC TCGCCGGACA CCAAAGAAAC TGGCGTTTCG CCCTTCTCCA GTCCGACGTC 
CTCGTCCAAG GACAAAAATA CCAGACCAAC CCCATGCGCC GCATCTTTGC GCCGGCTCGT 
GGTCTGTTTG TCGAGATTCT ACACCCGAAC AACCCCGAGA AGACGGTCAT TCTCGTCAAG 
GAGCAGCCAC GCCCCAACCG CTACGCTGAG GTCATTGAGG TCAAGCTGGA CGGCAAGAAC 
GAGATTGTAG TCAACATGAT CAAGGATACC ACGGCCCTCG GAAAGCCAGT GCCCCTCACT 
CTCAAGTTTA TCTACAACCC CGAGGCTGGC TATGCTCCTA TTCATGAGGT TATGGATGGT 
CGCAATGATC GTATCAAGGA GTTCTACTGG CGCGCGTGGT TCGGCGATGA GAAGCTCGAC 
CTCGATTCCT CCGTTCATGA AGTCTTCGAC GGCGGCAAGA CCACCATCAC CAGCGAGGCC 
ATCAATGAGT TTGTTCACGC CGTTGGCAAC ACCGGAGAGG CCTTCGTTGA CAGACCCGAC 
AAGGTCATGT ATGCTCCCAT GGACTTTGCT ATCGTTGTTG GCTGGAAGGC TATCACCAAG 
CCTATTTTCC CTCGCACCAT TGACGGTGAC CTGCTCAAGC TCGTCCATTT GTCCAACCAG 
TTCCGCATGA TGCCTGGTGC TGAGCCCCTC AAGAAGGGTG ATGTGGTTTC GACTACTGCT 
CAGATCAACG CTGTCATCAA CCAGGAGTCT GGAAAGATGG TCGAGGTCTG TGGTACCATC 
ACTCGTGACG GTGAGAAGGT CATGGAGGTT ACTTCGCAGT TCTTGTATCG TGGTACCTAC 
ACCGACTTCG AGAACACATT CCAGCGCAAG GTGGAGACCC CTATGCAGCT TCATCTTGCC 
ACCTCCAAGG ACGTGGCCGT CCTTCGCTCC AAGGATTGGT TCATGATGGA TGATGTTGAA 
AACCCTGATA TTGAGCTTCT CGGCCAGACC CTCACCTTCC GTCTCCAGAC CTTGGTTCGC 
TTCAAGAACA AGACTGTCTT CAGCCACGTG GAGACCCAGG GCCAGGTTCT TCTTGAGCTC 
CCGACCAAGG AGATTATCCA GGTTGCCACT GTCAACTACT ACGCTGGAGA ATCTCACGGA 
AATCCCGTCA TCGACTACCT CCAGCGTCAT GGTTCCTCCA TTGAACAGCC GATCAACTTT 
GAGAACCCTA TCCCTCTGAG CGGAAAGACC CCCCTCCAAC TCAAGGCGCC CTCGTCCAAC 
GAGACTTATG CCCGCGTCTC CGGTGATTAT AACCCCATTC ACGTTTCTCG TGTCTTTTCC 
AGCTATGCCA ACCTTCCTGG CACCATCACT CACGGCATGT ACTCGAGTGC CGCGGTGCGC 
AGCTTGGTTG AGACCTGGGC TGCCGAGAAC AAGGTTGGCC GCGTTCGCAG CTTCCACGCC 
TCTCTTACCG GCATGGTTCT GCCCAATGAT GATATCAACG TGAAGTTGCA GCACGTCGGC 
ATGGTTGCTG GCCGGAAGAT TATCAAGGTT GAGGCTAGCA ACAAGGAGAC CGAGGAAAAG 
GTGCTCCTGG GCGAGGCCGA AATTGAGCAG CCTATCACCG CTTACATCTT CACCGGCCAG 
GGCTCGCAGG AGCAGGGCAT GGGTATGGAT CTTTATAACA GCAGCCCTGT GGCCAAGGCT 
GTCTGGGATC GGGCCGACAA GTATCTCATG GACACTTATG GCTTTGCCAT CACCAACATT 
GTCCGGAACA ACCCCAAGGA GCTCACTATT CACTTTGGTG GCCCTCGTGG CAAGGCCATT 
CGCCAGAACT ACATGTCCAT GACTTTCGAG ACTGTGGCTG CCGATGGCTC GATCAAGTCG 
GAGCGCATGT TCAAGGAGAT CGACGAGAAG ACTACCTCTT ACACTTACCG CTCACCCAAC 
GGTCTTCTCT CGGCTACCCA GTTCACGCAG CCCGCTCTTA CTCTAATGGA AAAGGCCAGC 
TTCGAGGACA TGAAGGCCAA GGGACTTGTG CCCCGTGATA GCACCTTCGC TGGTCACTCT 
CTTGGCGAAT ACTCTGCGCT GGCTGCCCTT GCCGATGTCA TGCCCATCGA GTCCCTCGTG 
TCCGTCGTCT TCTACCGTGG TCTCACCATG CAAGTCGCTG TCGAGCGCGA TGAGACAGGC 
CGCTCCAACT ATGGCATGTG CGCTGTCAAC CCCAGCCGTA TCAACAAGAC TTTCACCGAA 
GAGGCCTTGC GGTTCGTGGT CGGTAGCATC GCAGAGACCA CCGGATGGCT TCTTGAAATC 
GTCAACTTCA ACGTCGAGAA CATGCAGTAC GTCTGTGCTG GCGACTTGCG TGCGCTCGAC 
ACCCTTGGCG GTGTGTGCAA CGCTATCAAG ATGATGAAGA TCGACATTGA GAAAATGCGC 
AAGGAGTACT CTGCCGAGAC TGTCAAGGAG CAGCTGGTCG AGATCATCAA GTCGTGCGCC 
AACGACACTC AGGCTAAGCC CACTCCCCTC GTTCTCCAGC GTGGCTTTGC AACTATCCCG 
CTCCCCGGTA TCGATGTGCC CTTCCACTCG ACATTCCTGC GCTCTGGTGT CAAGCCTTTC 
AGAAGCTTCT TGCTCAAGAA GATCAACAAG ACTACCATCG ACCCATCGAA GCTTATTGGC 
AAGTACATTC CCAACGTGAC TGCTAAGCCA TTCGCAATCA CCAAGGAGTA TTTCGAGGAT 
GTCTACCGGT TGACGAACTC CCCTAAGATC CAACAGATCT TGGCAAACTG GGATAAGTAC 
CAGGAGGAGG GTGTCAGTGG AATCCCTGAT GCGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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cel-2 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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