ORF » Species Summary » Schizosaccharomyces pombe » eng1 cDNA ORF clone

eng1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following eng1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eng1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc103146 NM_001018560.2
Latest version!		 Schizosaccharomyces pombe endo-1,3-beta-glucanase Eng1 (eng1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    eng1 ( NM_001018560.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593162.1 Schizosaccharomyces pombe endo-1,3-beta-glucanase Eng1 (eng1), mRNA.
    Price & Availability↑
    CloneID OSc103146
    Clone ID Related Accession (Same CDS sequence) NM_001018560.2
    Accession Version NM_001018560.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3051bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product endo-1,3-beta-glucanase Eng1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018560.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGTTCCT ATTTACGTTC TTTTATATTT GGTCTTTTGA CCATAAGTTT GGCACAATGC 
    TCGCCAATCC TTAAGGATAC CAAGGATACC AAATTTTCCA CAGGCTCTAA TATTAGCTTA 
    AAAAAACGTG ATACCAATGT TTTCGACTCT GTTGTTGATA CTATCAACCC AGCCAGTTAT 
    TTCGGCACCG TTTCGCACCC AGTTACTCCT GCCGGCGTCA GTACTGACAG CTTGAGCTCC 
    CCTATTGAAA CTAACAAATT TTTTGATAAT AATTTACTTG GTTCTCGTAC TAATTTCATG 
    TATGCTGATC CCTTCAGATA TTGGTGGCAG TCTTCAGACA CGATGGGTGG CATTTGTATT 
    GCTCATACCG ATGACAATCA ACGTGTAATG GATACGGACG ATACAATCCC TTCCTATTAT 
    TATGAACCCA TCGGTATTTG TTCGCTCGGA TTTGGTGCTT CTGGTATTAC CTCTAATACT 
    GACCCAATTG TCGACGAGAT TGATCAGATG TCTGCTCGCT TTACTTTCTC TTGGGACTCT 
    TCGTCTATGC AACTTACTTT GACTGAGGGT ATGGCCGTTA CTACTGCTGT CTACACTAAT 
    GCTATTCCTC AAATTTTTTC ATCCACTCTT TATATCAATG ACTTTGTCGA AGTGCCGGGT 
    ACTTCTGCTG TTCAAAAGTA TCGTGTCACG ATGTCTGATA ACCACGTTTG GCTTATTTAC 
    ATTTATGGGG ATAGCCTTAC GTTGACTGAA TCCACATCTC AAATGCTTGT TGGAAGCAAC 
    ACTTTCAATG GTTACATTCA AATCGCGAAA ATTCCTCTTG GAGATGGCAC CGCTGAAGCT 
    CTTTATGACA CTTATGCGGG TGTCTATATT ACCGGTATTA GTATTTCCGG ATATGTTGAG 
    GATGCTGTTG GTTATTACTC GTTTGATTTT ACGACTGCTG GGGATACTTC AGTAGAGCCT 
    CTATTTTTCT TATTGCCTCA TCAAGTGGAT ACAGCTGTCA GTGGTACCAA AGTTACATCC 
    ATTGTTTTGG CTTCTCTAGT AAGTGGTGAT ATGAATGCCG CAGCTGGAAA CTCTATAACA 
    TTCGCCGAAG CTATTCCTCA GGACATTGGT TTTCTTCCAT GGTCGCCGAC AGGTGGTCAG 
    ATTGGTTATT CGGAAGAAGC ATTAGAGATC ATCGCTGAGG TTGCTGGTAC TGAATTGGGC 
    GAAGACTTCT CTGCCAACTC TAATTTGAAT TCAATGTACT ATTCTGGCAA AGTTTTGGCT 
    AAGTATGCAA TGCTGTGTGT TACAATTAAT GATATTCTTG GTGATGAGAC CAGCAGCGAA 
    CAATGTATTC AAAAACTAGA GGCTGCTTTT GCCCGTTTTG TTGACAATCA GCAAATTTAC 
    CCTCTGACTT ATGATAACAC TTGGAAAGGA GTTGTCAGTG TAGCTGGTCT ATCTGGTGAT 
    TCCTTGGCTG ATTTTGGTAA CAGCTACTAT AATGATCACC ATTTCCATTA TGGTTATTTT 
    GTTTTCACTG CTGCTGTAAT TGGTCATATT GATCCTGACT GGATTAACAC TGGGAACAAT 
    AAGGAATGGG TTAACTTTCT AGTTCGTGAT GTTGCCAATC CATCATCAAA TGATCCTTAC 
    TTTCCAAAGC ACCGAATGAT TGATATTTAT CATGGTCATG GTTGGGCATC TGGTTTGTTT 
    GAGTCTAATG ATGGCAAGGA TGAAGAATCA ACGTCCGAAG ATTATAACTT CTTTTTCGGT 
    ATGAAACTTT GGGGTCAAGT AATTGGCGAT TCTGATATGG AGGATCGCGC AAACATTATT 
    TTGGGCATTG AAAGAAATGC TTTGAATAAG TACATGCTGT ATGCTGATGG AAACGTCCAG 
    CCCACTTCTA TGCAACCTAA TTATGTTGCC GGTATTACAT TCATGAACAA AATTACTCAC 
    ACTACCTATT TTGGTACTAA TATTGAGTAC ATCCAAGGAA TTCATATGTT GCCTATCACT 
    CCAATTTCAG CTTTTATCCG TGGTCCTAGC TTTGTACTAG CTGAATGGAA TGCCTTACTT 
    GCTAGTGTCA TTGACTATGT AGATAGCGGT TGGAGAAGTC TTTTATATGC TAATCTAGCT 
    ATTGCTGAAC CTGAAGAAAG TTATGAATAT TTCTCCAGTT CTGATTTTAA TACCGATTAC 
    TTAGATGACG GGGCCTCTCG TGCCTGGTAT TTGGCTTATG CCGCTGGCTT ATGGGCTAAT 
    GATGCCGTTT ATTATCCTGT TAGCTCTTCT TCTACCACCA CGACCTCTAC GAGTACCGGA 
    AGTGTTACGA CAACCTCTAC TACTGCAACT GCCAGTTGTA CACTACCTAT ATCCTATACT 
    TCGACTCCTA CTACTACAAG CATCTCTGGT ACTTGTAATG GTGCTACGTT TGATGCAAGT 
    TTGTATGTAT GCGACGGAAC TGTACTCTGC CCTATTGTTA ATGGTGTATC ATATCAAAAC 
    TGCAACGGAG CTTGCTATAA CCCTTCGCAA TATGGCTGCG ACAATGGAGC TTTGGGCCCT 
    GTTCAATCTT CATCGACCAC TTCCTCCATT ACGCCTACTC CCACAACTAC TTCCTCAATA 
    ACACCCACAC CTACTACAAC TTCAACAACT ACTACCGCAC AGTCAACTGG CATGCAACTA 
    TGTGGCTCTA ACTACTACGA CGCATCCTCT TATTATTGTG ACAATGATCA GTTATGTCCT 
    ATAATAGACG GAGTGGATTA CTTGAGTTGT AATGGTGCAT GCTATAATCC TTCCCAATAT 
    GTTTGTTCTG ATGGTTCTTT GTCTCCAAAC ACTGTTACAA CAACAAAAGC TACTACAACT 
    TTCACACCTA CACCTACCAC AACTACTACA CCTACACCTA CTACTACATC TGCTACATCC 
    ACTAATGTGA TAGCCCAATG TGGATCAGCT TGGTATGATT CACAAAGTTA TATTTGTTAT 
    GGCAATATAC TGTGTCCCAT TATAAATGGG TCTCCATTGT TAGCTTGTGG TAATGCGTGC 
    TATGACTCCT CTATATACGG TTGCTCCAAT GGTGCACTTG TTGCTGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_593162.1
    CDS268..3318
    Translation

    Target ORF information:

    RefSeq Version NM_001018560.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe endo-1,3-beta-glucanase Eng1 (eng1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001018560.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGTTCCT ATTTACGTTC TTTTATATTT GGTCTTTTGA CCATAAGTTT GGCACAATGC 
    TCGCCAATCC TTAAGGATAC CAAGGATACC AAATTTTCCA CAGGCTCTAA TATTAGCTTA 
    AAAAAACGTG ATACCAATGT TTTCGACTCT GTTGTTGATA CTATCAACCC AGCCAGTTAT 
    TTCGGCACCG TTTCGCACCC AGTTACTCCT GCCGGCGTCA GTACTGACAG CTTGAGCTCC 
    CCTATTGAAA CTAACAAATT TTTTGATAAT AATTTACTTG GTTCTCGTAC TAATTTCATG 
    TATGCTGATC CCTTCAGATA TTGGTGGCAG TCTTCAGACA CGATGGGTGG CATTTGTATT 
    GCTCATACCG ATGACAATCA ACGTGTAATG GATACGGACG ATACAATCCC TTCCTATTAT 
    TATGAACCCA TCGGTATTTG TTCGCTCGGA TTTGGTGCTT CTGGTATTAC CTCTAATACT 
    GACCCAATTG TCGACGAGAT TGATCAGATG TCTGCTCGCT TTACTTTCTC TTGGGACTCT 
    TCGTCTATGC AACTTACTTT GACTGAGGGT ATGGCCGTTA CTACTGCTGT CTACACTAAT 
    GCTATTCCTC AAATTTTTTC ATCCACTCTT TATATCAATG ACTTTGTCGA AGTGCCGGGT 
    ACTTCTGCTG TTCAAAAGTA TCGTGTCACG ATGTCTGATA ACCACGTTTG GCTTATTTAC 
    ATTTATGGGG ATAGCCTTAC GTTGACTGAA TCCACATCTC AAATGCTTGT TGGAAGCAAC 
    ACTTTCAATG GTTACATTCA AATCGCGAAA ATTCCTCTTG GAGATGGCAC CGCTGAAGCT 
    CTTTATGACA CTTATGCGGG TGTCTATATT ACCGGTATTA GTATTTCCGG ATATGTTGAG 
    GATGCTGTTG GTTATTACTC GTTTGATTTT ACGACTGCTG GGGATACTTC AGTAGAGCCT 
    CTATTTTTCT TATTGCCTCA TCAAGTGGAT ACAGCTGTCA GTGGTACCAA AGTTACATCC 
    ATTGTTTTGG CTTCTCTAGT AAGTGGTGAT ATGAATGCCG CAGCTGGAAA CTCTATAACA 
    TTCGCCGAAG CTATTCCTCA GGACATTGGT TTTCTTCCAT GGTCGCCGAC AGGTGGTCAG 
    ATTGGTTATT CGGAAGAAGC ATTAGAGATC ATCGCTGAGG TTGCTGGTAC TGAATTGGGC 
    GAAGACTTCT CTGCCAACTC TAATTTGAAT TCAATGTACT ATTCTGGCAA AGTTTTGGCT 
    AAGTATGCAA TGCTGTGTGT TACAATTAAT GATATTCTTG GTGATGAGAC CAGCAGCGAA 
    CAATGTATTC AAAAACTAGA GGCTGCTTTT GCCCGTTTTG TTGACAATCA GCAAATTTAC 
    CCTCTGACTT ATGATAACAC TTGGAAAGGA GTTGTCAGTG TAGCTGGTCT ATCTGGTGAT 
    TCCTTGGCTG ATTTTGGTAA CAGCTACTAT AATGATCACC ATTTCCATTA TGGTTATTTT 
    GTTTTCACTG CTGCTGTAAT TGGTCATATT GATCCTGACT GGATTAACAC TGGGAACAAT 
    AAGGAATGGG TTAACTTTCT AGTTCGTGAT GTTGCCAATC CATCATCAAA TGATCCTTAC 
    TTTCCAAAGC ACCGAATGAT TGATATTTAT CATGGTCATG GTTGGGCATC TGGTTTGTTT 
    GAGTCTAATG ATGGCAAGGA TGAAGAATCA ACGTCCGAAG ATTATAACTT CTTTTTCGGT 
    ATGAAACTTT GGGGTCAAGT AATTGGCGAT TCTGATATGG AGGATCGCGC AAACATTATT 
    TTGGGCATTG AAAGAAATGC TTTGAATAAG TACATGCTGT ATGCTGATGG AAACGTCCAG 
    CCCACTTCTA TGCAACCTAA TTATGTTGCC GGTATTACAT TCATGAACAA AATTACTCAC 
    ACTACCTATT TTGGTACTAA TATTGAGTAC ATCCAAGGAA TTCATATGTT GCCTATCACT 
    CCAATTTCAG CTTTTATCCG TGGTCCTAGC TTTGTACTAG CTGAATGGAA TGCCTTACTT 
    GCTAGTGTCA TTGACTATGT AGATAGCGGT TGGAGAAGTC TTTTATATGC TAATCTAGCT 
    ATTGCTGAAC CTGAAGAAAG TTATGAATAT TTCTCCAGTT CTGATTTTAA TACCGATTAC 
    TTAGATGACG GGGCCTCTCG TGCCTGGTAT TTGGCTTATG CCGCTGGCTT ATGGGCTAAT 
    GATGCCGTTT ATTATCCTGT TAGCTCTTCT TCTACCACCA CGACCTCTAC GAGTACCGGA 
    AGTGTTACGA CAACCTCTAC TACTGCAACT GCCAGTTGTA CACTACCTAT ATCCTATACT 
    TCGACTCCTA CTACTACAAG CATCTCTGGT ACTTGTAATG GTGCTACGTT TGATGCAAGT 
    TTGTATGTAT GCGACGGAAC TGTACTCTGC CCTATTGTTA ATGGTGTATC ATATCAAAAC 
    TGCAACGGAG CTTGCTATAA CCCTTCGCAA TATGGCTGCG ACAATGGAGC TTTGGGCCCT 
    GTTCAATCTT CATCGACCAC TTCCTCCATT ACGCCTACTC CCACAACTAC TTCCTCAATA 
    ACACCCACAC CTACTACAAC TTCAACAACT ACTACCGCAC AGTCAACTGG CATGCAACTA 
    TGTGGCTCTA ACTACTACGA CGCATCCTCT TATTATTGTG ACAATGATCA GTTATGTCCT 
    ATAATAGACG GAGTGGATTA CTTGAGTTGT AATGGTGCAT GCTATAATCC TTCCCAATAT 
    GTTTGTTCTG ATGGTTCTTT GTCTCCAAAC ACTGTTACAA CAACAAAAGC TACTACAACT 
    TTCACACCTA CACCTACCAC AACTACTACA CCTACACCTA CTACTACATC TGCTACATCC 
    ACTAATGTGA TAGCCCAATG TGGATCAGCT TGGTATGATT CACAAAGTTA TATTTGTTAT 
    GGCAATATAC TGTGTCCCAT TATAAATGGG TCTCCATTGT TAGCTTGTGG TAATGCGTGC 
    TATGACTCCT CTATATACGG TTGCTCCAAT GGTGCACTTG TTGCTGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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