rad22 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	rad22
Entrez Gene ID	2543086
Full Name	DNA recombination protein Rad22
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018603.2	NP_593207.1	DNA recombination protein Rad22

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rad22 plays dual roles in regulating meiotic recombination: activating Rad51 and inhibiting Dmc1.
Title: Dual regulation of Dmc1-driven DNA strand exchange by Swi5-Sfr1 activation and Rad22 inhibition.

Rad52 protein function study by fluorescne tagging.Rad22 protein tagging,a S. pombe is a Rad52 homologue.Presence of tagged Rad22 protein was confirmed by Western-blot and fluorescence microscopy.
Title: Rad52 protein function study by fluorescence tagging.

mediates the formation and branch migration of Holliday recombination intermediates. (review)
Title: [Molecular basis of the formation and branch migration of Holliday recombination intermediates mediated by eukaryotic recombinases].

The most important function of Rad22 and Rti1 in S. pombe meiosis is repair of double-strand breaks with involvement of the sister chromatids.
Title: The Rad52 homologs Rad22 and Rti1 of Schizosaccharomyces pombe are not essential for meiotic interhomolog recombination, but are required for meiotic intrachromosomal recombination and mating-type-related DNA repair.

Gel-filtration experiments and electron microscopic analyses indicate self-association of Rad22A and Rad22B and the formation of multimeric structure.
Title: Schizosaccharomyces pombe Rad22A and Rad22B have similar biochemical properties and form multimeric structures.

The following rad22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rad22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103677	NM_001018603.2 Latest version!	Schizosaccharomyces pombe DNA recombination protein Rad22 (rad22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rad22 ( NM_001018603.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593207.1 Schizosaccharomyces pombe DNA recombination protein Rad22 (rad22), mRNA.

Price & Availability↑

CloneID	OSc103677
Clone ID Related Accession (Same CDS sequence)	NM_001018603.2
Accession Version	NM_001018603.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	DNA recombination protein Rad22
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018603.1.

ATGTCTTTTG AGCAAAAACA GCATGTAGCA TCAGAAGACC AGGGCCATTT TAACACAGCG 
TATTCTCATG AAGAGTTTAA CTTTCTTCAA AGCTCCCTAA CTCGAAAGCT TGGACCTGAG 
TACGTTTCAA GAAGGTCAGG TCCTGGGGGT TTTTCAGTCA GTTATATTGA GTCTTGGAAA 
GCAATTGAGC TTGCGAACGA AATTTTTGGA TTTAATGGGT GGTCTTCGTC GATACGCAGC 
ATTAATGTTG ACTTTATGGA CGAAAATAAG GAAAATGGAC GTATCAGTCT TGGCTTGAGC 
GTGATTGTTC GCGTGACAAT CAAAGATGGT GCCTATCATG AAGATATTGG ATACGGTTCT 
ATTGATAATT GCCGTGGAAA GGCTTCAGCG TTTGAGAAAT GCAAGAAAGA GGGCACTACA 
GATGCCTTAA AACGCGCTCT ACGAAATTTT GGAAACAGTT TAGGAAATTG CATGTATGAC 
AAATACTATC TTAGAGAAGT TGGAAAAATG AAACCACCCA CTTATCACTT TGATAGCGGT 
GACTTGTTCC GTAAAACAGA TCCTGCAGCG AGAGAGTCTT TCATAAAAAA ACAGAAAACA 
TTAAATAGCA CGAGGACTGT TAACAATCAA CCTCTGGTTA ACAAGGGAGA ACAATTAGCT 
CCTCGTAGAG CTGCAGAATT AAATGACGAA CAGACAAGAG AAATTGAAAT GTATGCAGAC 
GAAGAGCTAG ACAATATTTT TGTAGAGGAT GATATAATTG CTCATTTAGC AGTAGCAGAG 
GACACAGCCC ATCCTGCTGC TAATAACCAC CATTCTGAAA AAGCTGGCAC TCAAATAAAT 
AACAAAGATA AAGGTTCACA CAATTCAGCT AAACCAGTGC AGAGAAGCCA CACCTATCCT 
GTCGCTGTTC CACAAAATAC CAGTGATTCC GTTGGCAATG CTGTGACTGA CACTTCCCCA 
AAAACGCTTT TCGACCCACT TAAGCCAAAT ACTGGTACAC CTTCTCCAAA GTTTATTAGT 
GCACGGGCCG CAGCCGCAGC CGAAGGAGTT GTTTCAGCTC CCTTCACGAA CAACTTTAAT 
CCTCGTCTCG ACAGCCCTTC TATTAGGAAA ACCTCAATAA TCGATCATTC TAAATCATTA 
CCTGTACAGA GGGCATCGGT TTTACCTATA ATTAAGCAAT CTAGCCAAAC AAGCCCGGTT 
TCTAACAACT CTATGATACG TGACTCTGAA TCGATTATTA ACGAAAGAAA AGAAAACATT 
GGACTTATTG GAGTTAAGCG TTCTTTACAT GACTCAACGA CTTCTCATAA TAAAAGTGAT 
CTTATGAGAA CAAATTCTGA TCCTCAGTCG GCAATGAGGT CGCGAGAAAA CTACGATGCT 
ACGGTGGATA AGAAAGCCAA AAAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593207.1
CDS	47..1456
Translation	MSFEQKQHVASEDQGHFNTAYSHEEFNFLQSSLTRKLGPEYVSRRSGPGGFSVSYIESWKAIELANEIFGFNGWSSSIRSINVDFMDENKENGRISLGLSVIVRVTIKDGAYHEDIGYGSIDNCRGKASAFEKCKKEGTTDALKRALRNFGNSLGNCMYDKYYLREVGKMKPPTYHFDSGDLFRKTDPAARESFIKKQKTLNSTRTVNNQPLVNKGEQLAPRRAAELNDEQTREIEMYADEELDNIFVEDDIIAHLAVAEDTAHPAANNHHSEKAGTQINNKDKGSHNSAKPVQRSHTYPVAVPQNTSDSVGNAVTDTSPKTLFDPLKPNTGTPSPKFISARAAAAAEGVVSAPFTNNFNPRLDSPSIRKTSIIDHSKSLPVQRASVLPIIKQSSQTSPVSNNSMIRDSESIINERKENIGLIGVKRSLHDSTTSHNKSDLMRTNSDPQSAMRSRENYDATVDKKAKKG

Target ORF information:

RefSeq Version	NM_001018603.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe DNA recombination protein Rad22 (rad22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018603.2

ORF Insert Sequence:

ATGTCTTTTG AGCAAAAACA GCATGTAGCA TCAGAAGACC AGGGCCATTT TAACACAGCG 
TATTCTCATG AAGAGTTTAA CTTTCTTCAA AGCTCCCTAA CTCGAAAGCT TGGACCTGAG 
TACGTTTCAA GAAGGTCAGG TCCTGGGGGT TTTTCAGTCA GTTATATTGA GTCTTGGAAA 
GCAATTGAGC TTGCGAACGA AATTTTTGGA TTTAATGGGT GGTCTTCGTC GATACGCAGC 
ATTAATGTTG ACTTTATGGA CGAAAATAAG GAAAATGGAC GTATCAGTCT TGGCTTGAGC 
GTGATTGTTC GCGTGACAAT CAAAGATGGT GCCTATCATG AAGATATTGG ATACGGTTCT 
ATTGATAATT GCCGTGGAAA GGCTTCAGCG TTTGAGAAAT GCAAGAAAGA GGGCACTACA 
GATGCCTTAA AACGCGCTCT ACGAAATTTT GGAAACAGTT TAGGAAATTG CATGTATGAC 
AAATACTATC TTAGAGAAGT TGGAAAAATG AAACCACCCA CTTATCACTT TGATAGCGGT 
GACTTGTTCC GTAAAACAGA TCCTGCAGCG AGAGAGTCTT TCATAAAAAA ACAGAAAACA 
TTAAATAGCA CGAGGACTGT TAACAATCAA CCTCTGGTTA ACAAGGGAGA ACAATTAGCT 
CCTCGTAGAG CTGCAGAATT AAATGACGAA CAGACAAGAG AAATTGAAAT GTATGCAGAC 
GAAGAGCTAG ACAATATTTT TGTAGAGGAT GATATAATTG CTCATTTAGC AGTAGCAGAG 
GACACAGCCC ATCCTGCTGC TAATAACCAC CATTCTGAAA AAGCTGGCAC TCAAATAAAT 
AACAAAGATA AAGGTTCACA CAATTCAGCT AAACCAGTGC AGAGAAGCCA CACCTATCCT 
GTCGCTGTTC CACAAAATAC CAGTGATTCC GTTGGCAATG CTGTGACTGA CACTTCCCCA 
AAAACGCTTT TCGACCCACT TAAGCCAAAT ACTGGTACAC CTTCTCCAAA GTTTATTAGT 
GCACGGGCCG CAGCCGCAGC CGAAGGAGTT GTTTCAGCTC CCTTCACGAA CAACTTTAAT 
CCTCGTCTCG ACAGCCCTTC TATTAGGAAA ACCTCAATAA TCGATCATTC TAAATCATTA 
CCTGTACAGA GGGCATCGGT TTTACCTATA ATTAAGCAAT CTAGCCAAAC AAGCCCGGTT 
TCTAACAACT CTATGATACG TGACTCTGAA TCGATTATTA ACGAAAGAAA AGAAAACATT 
GGACTTATTG GAGTTAAGCG TTCTTTACAT GACTCAACGA CTTCTCATAA TAAAAGTGAT 
CTTATGAGAA CAAATTCTGA TCCTCAGTCG GCAATGAGGT CGCGAGAAAA CTACGATGCT 
ACGGTGGATA AGAAAGCCAA AAAAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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rad22 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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