ubr11 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	ubr11
Entrez Gene ID	2542809
Full Name	N-end-recognizing protein (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019721.2	NP_594298.1	putative N-end-recognizing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors show that Schizosaccharomyces pombe has the N-end rule pathway in which only Ubr11, but not Ubr1, is responsible.
Title: Essential role of Ubr11, but not Ubr1, as an N-end rule ubiquitin ligase in Schizosaccharomyces pombe.

Mutations in the UBR box and the autoinhibitory domain blocked degradation of both type-1 and type-2 substrates, expression of peptide transporter genes, and the uptake of oligopeptides.
Title: The type-2 N-end rule peptide recognition activity of Ubr11 ubiquitin ligase is required for the expression of peptide transporters.

Consistent with its role in transcriptional regulation of peptide transporter genes, the Ubr11 protein is accumulated in the nucleus.
Title: The Ubiquitin ligase Ubr11 is essential for oligopeptide utilization in the fission yeast Schizosaccharomyces pombe.

The following ubr11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ubr11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102218	NM_001019721.2 Latest version!	Schizosaccharomyces pombe putative N-end-recognizing protein (ubr11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ubr11 ( NM_001019721.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594298.1 Schizosaccharomyces pombe putative N-end-recognizing protein (ubr11), mRNA.

Price & Availability↑

CloneID	OSc102218
Clone ID Related Accession (Same CDS sequence)	NM_001019721.2
Accession Version	NM_001019721.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative N-end-recognizing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019721.1.

ATGCAATTGC ACGACCCGCC GGCTCCTCTT TCGCAACCAT CGGCTTCTAG GTTACAAAAA 
TACCTTCTAG AAAGCGCGGA AAAGCATGCA TACGATTCAA ATGAAGAATC AAAAACGCAC 
TTGCTGCAAG AGGTTTTCCT GTCGTTGCTG AATTACAACG AAGACAATTG GAAATATTTT 
CTCAAAGAAA AGCCAGGTGC CATTACTTCT GACTTTCGTC TCTCGAGGCT TCAGCACTCT 
GAACCCGAAT GCGCCCAAGA ATTACAAGAT AAACGTTCTG GGTCCAAGGT TTGTGGCCAC 
GTATTTCGTG CCGGCGAGGT GATATATCGT TGCAAGAACT GTGGGCTAGA TAACACATGT 
GTTCTTTGTG CTCCTTGCTT TCATGCCACA AACCACGAGG GTCACGAGAC TCATGTATCT 
ATTTCAACCT CTTATAGTGG AATCTGCGAC TGTGGCGATC CTGAGGCATG GAATGTCGAC 
CTTAACTGCA AGATACATAA TGTCCCCGAT GACGAGGAGC AGAAAAAGCC AGAAGAAGTG 
ATCCCACTAG AACTTCAACA CTCTATCCGT ACAACTATTC ATATTCTTCT TGACTTCATT 
CTCGATGTCT TTTCTTGTTC CCCCGTGAAT CTTAAAGCTC AATCTACTGT CGGTAGCATC 
TTAGCAGATG AGGAGGCCTC TCGTCTTAGC AGCGCAAAGT ATGGTGTAGC TGATAGGCCT 
TGCAACGTTT TCCGTGTAAT GCTTTGGAAC GACGAAGTGC ATACCTTTGA CGCTGTTGTA 
GGTAGTGTGC TTGAGGCGTT AGATTCGTCA AATACGGCTT TTGGACTTGA AGTCGCTCAA 
CGTGTTGATT CTATTGGCCG CTTTGCTGTT GCTACATCGG CTTCAGTTCA CGAAGCAATA 
AGAATCGCGA ACGCTATTTC AAAAGAAAAC TTAGCTGTTA ATGTTCGTAC TGCCCGCGAT 
TTTTTTCGCG AAGATATTTG TGGTATTCTA TTAGAATGGT TCGATGATCT TCTAGAATCA 
CATGTTTGCT ATTTTGCTGA TTATTTACAA ATTATCGTTT GCGATGAAAT TTTGAAGAAC 
TGGTCTCCTG GCCTTGAAAA ACCTGCTAAG CCTGAGGTGA ATTTTAATAA CCTTCCTCTG 
GAGATTGTTA ATGACGATGA TAGCGAGGAT GATATTTATG CCGCAGAAGA ACTTCTCGAT 
GTTATCGCAA ACCTTCAAGA TGAAACAGGT GTAACTAGAA TTGCTAATTT GGGTGGTGAT 
GAAGATTTTG AAGCGGATAT GACCGATCCT ACTATTGCAG GATTTGATCA TCCACTTGAT 
GATGATAACG ACGTAAATGA CCTTCTGGAT TTTGAAACAG AACGAGAGGA TATTGATGAT 
TTAACTGATG AAGTTATGGA GACAGAAGAA AATGAAGCTG CTGAGGCTGA TTATCCTGGT 
GTAAACCGAA ACACTCGTCA AGACGATGTT CAAGATATTT CAATGGAAAC CGAAAGTCAA 
AACGAAACTG ATGAATCACA AAACACTGAA AATGTAGATT ATAATCCTCA GACGCATACT 
CCTGTACCTA TTCCCACTAC TGCAACTCAG GATGTCGTTA CTATTCGCCC GGAGTTTAAC 
TCTCAACTCC TTAATAACCT TCGACAAATC ATAAACGCTC GTCGTAGACC CCGCCCTGCT 
GCGGTTTGTC AAGTATCTCT TCGTGAGGAT TATTGGAAAA GTCCTCATCC AATTCCTCCT 
TCGTCATATA GCTTCGTTGA ATCTCCATCT TCCATTCTTC GTTTAGATTA CTTTTTGCTG 
TTTGATCTCA AATTTTGGAA ACGGTTAAGA GGGCTGCTCT CAAAATTGTA TGTTGTTCCT 
TTTAATAGAA ATCTTCTATT TAAGCGTCTT ATGGGTATAC GTTTCGTAAT CCATTATAGG 
TCTCTCGCTA CAGCGTTTCT TTTTGCAGAT AGAGAGCCAG ACCACTCTGT CATGTTTCTT 
TCCGTTCAAT TTTTCACTAC TCCTAGTCTT GCAGAAGCCG TTGTGAAAGA CTATGATTTT 
TTAACTAATT TAAACGCAAC AACTTTAAGC TTACTAACGC AGTCTAACCG GCCGTCAACC 
CTTTTTTCTT CAGACATTGA ATACACTCCC ACAATTCAGC TAAACCGACA AGTCTTAAAA 
ACCCGAAGGA CTTACAATTT GTTTAGTGAT CTCGGATACT TACTTCAACA TCCGCAAGTC 
AAAAAGCTTG TAGTGGATGA TACGCGTTAT GTTCATCAGT ATATTGATTT GTTACGGGTG 
TTCCAAGGTG TGATTCCACA ACAACGGGCA ATACTCAGTC ACGTACAGTG GGATTTTCCT 
CATGGGAAAA ATATATTGTT TGTAATGCAA AGGGTAGCTA TGCTCAGTAA CACTGTTTCA 
TCTTGTTTTA CACAAGCACC ATATGAACGG TTGTTTTATG CTATAAAATG TATCATAACT 
TCCATCACCC ACCCTAAATT AGACATTGCA GAGTCTCTCG AACCTCTCTC TTGTATTCCC 
TCTTCATCTT TGACAAATTT TACACAGCCC TTAGTTCCGT TTTCCGTGTC GAGGGATCCT 
ATAAGTTTTT ATCATCCTCT TCACTGGATG CTTTCCAATT TATTTTCATA TTGTCGTGTT 
GACGCTTCTT CTCATTGGGA TAAGGATACA CTACTTGCTC TTCTTGACCA TCCTTTGAGG 
GTTTGTGTGC TTTTAGCTCA AATTGATTGT AACCTTTGGA TAAGAAATGG GCGTAGTATT 
TTATTAACTG ACGCTTTCTA TCGTCAACTT AATAACATCG AAGTTTCTTA TGATAAGGAC 
ATCCTCGCTA TTCAAACAAT ATTAATGTTT GTTGATCCAA ACTTGGTTTT AAATGCAGTT 
GTTCAAAGAT TTGAATTTAC TGATTGGCTT TATAATTTGA CTTATAATGA ACATCCCAAT 
TACGACACGG AAAGAATACC AGCTATGTTG TGTAAAATGT TAGAGTTATT AATTGCCCTT 
ATAACAGAGC GCGAACAAAT TTTACATGTT GATATCCAAG ATATTATTCG CACTCGATTA 
GCTCAACAGC TATGCTTTGG TCCTCTTGCT TATTCTGCAT TGCTGTCTAC TATTTCAAGT 
AATTTAGTAG AGAGTTTGTC ATTTGATAAA ATTCGAGAAG AAGTAACTTC CTACAAAGCA 
CCTGACGGTC TCCACGATTT TGGCGTTTAC AGTTTGAAGG ACGAATATTA TGATTTGGTG 
GATCCATATT ATTTTCATTA TAATAAAAAT GAACGAGAGG AGTCAGATAC TATCCTTAAA 
AAACGTTTGG CGAAGAAAAA TAATGTAAGT GCTGAGTCGA TTATAATAGA ACCAAAAATT 
AGATTTCTTG AGAAGGACGG CCATGATATA TTTTTTGCAG CCGTCAATGC GAGCACTTTT 
AGTCTTATCA TTTTTCGTGC GATTGAGTAT GCACTTGTTC AAGCGGAGTC CTTTGGATCA 
TCCGATATTG GAAATACTAT TCTGGGCGAT GCACTTCAGT TGTGTCTAAT CTCTATGAAA 
ATTCATGAAT TCTCGAAATC AAATGACTTC TGCTCCCGTT CATGCGCTGA GCGTTATCCG 
ACTGATAGTA GTATTATGAG AGAATTTGGT GGCAGTGCGT ACTGCTTAGC GGAACTTTGT 
TTTGCAATTC TCAAGTCTCC TAAATACAAA GATGTTCATG TAAAAGTTAA TGCTGTATTG 
GCTGGCTTAC AAAAGAATGA TCCATCTGCA TATAGCAACA TGCTGGAAGC TACACACTTT 
GAACTCTCAA CTACCTCTTC TACTTCGGAT TCTAACGAGA TTGAGAAAAC GCAGGAAAAG 
AAAAGATTAG CTTTAGAGAA ACAGAAGAAA ATTATGCAGC AGTTTCGCGA TCAGCAAGCA 
TCTTTTCTTG CCCAAAATAC CGATTTCGAT ATCGGAGAAG ATCAAACGGA AGATGAAGTT 
ACAACAGAAG AGCCAGAGGA GGAAGTTAAG TACCATGAAC ATATCCGTGG TAATTGTTTG 
CTTTGCCAAG AGGAATGCAA CGATCAAGCA CCATATGGAG TGATCGGCAT TATTCAAGGA 
AGCTCATTAC TTCGAAAAAC AGATGTTCAC AGCGAAATCA TTTTGGATGA AATTTATAGT 
GTTCCCCCTA ATTTAGATAG GGAATCTCAT TCGAGGCCAT TTGGAAAAAA ATATGATACA 
GTTGTTTTCA ACCGTTCAAA AGATCGTTTA TTAAGTGCTT ATCCCCCAGG AAATAACATA 
AGAGGTGTTT TTGTCTCCGG TTGTGGTCAT CTCATGCATT TAGGTTGTTT TAAAAACTAT 
TACGTTGCTA GGTCCATGTA TAGAAATGAT GTTACCGCTG GGTTAAGTGA ATACTATTAC 
AAGTATTCTA CTGCTAAATT TTTTATGTGT CCTCTCTGTA GGTCATTGTC GAATGTGTTA 
TTGCCTATGC CTCAGATCCC CAAAATGTGT CTCAACATTG ACACCCTTAA TTTCCCTCGT 
AGCATGAATG GTTGGTTAGA GGAAATCGGT ACTATGTCTT CCTCGAGCTT TGAATATCAA 
TTAGTTCGAA GTTCTCTGTC CGACACGAAA GATACTTTTA GAAGCTGTTT CCTGAGACCT 
TGGATAAACA GCAAAATTAT CTCGGCAATG CTTGCTCGCT TAAAGATAGC CGATGGTGCA 
TTAATTGATC AAAGTAATAA TCGGGACGTA TCCGACCTTT ATGATCGATA TTGTGAAACA 
ACAAAATTGG CTATGAAATT GGTTAAAGGA AGTACATTTA CGAATGTTTC ACCTCATGAT 
TTACTGAACA GTTTGGCCTA TACCGTTTCT TCTCTAGAAG TTAGTCAGAG GTGCTCTCCG 
AAACAAAGTG GAGCAACTCG ATCGGTGTGG TTCAATGAAC TGGGACCCCT AACATTATCG 
TTTTTGCCGA CTTTATCTGA TACCGTTTTG AAGTGTGTTT GTGATCAGAT CATAAAATCT 
GACCAACAAG CCCTCTTGTT AATGGAGAGC CAAAAACTGT TAGTTTGTAA AATTTTCTAT 
CGTCACTCGC AATTAAAGTC CATGTTGAGA AATGGCCGGA TGTCTGACCA TGATCAAATT 
CAACCGTTTT TGCTTTCAAA TACTTTCGAT GACTTTGTGA AAATTTCATC TTTAATGCTA 
ATCTTTGGTA AACAAGATAA TATTTTGTAT TACGTTAAGC TTTTCTATCT TTCAGAAATT 
TGCAAGACAA TAATTTCTAT GATAAAAGTG GTTGCAGATT CATCGGTCGT TCCTGATTTA 
ACCATTAATT ATAGTCAGCA ATCCAAGTCC CAATTTTATA TTCTATGCAA GAATGTACTG 
CTTTGGTGTG GTTCTTCAAA TAACATTGAA ATTTTGGATG ATGAGTCTAA TTTACTTCGT 
TTAATGTCTT TGGTGGAGAA GTATTCTTTA CCGTTTCTCC GACGAGTTGC TTTGGTATTA 
TATTGCATGT TTGACATTTC ACTCGAGTTC AATGAATTCT CCAACAATGA AGATGACAGT 
GAATTAGAAA GGCTTTCCAA ATTAATAAAA GTTCCTCCTT TACAAGAACT GTATTCTCAA 
ATGTCTTCTG ATGAAAATAA TCAGATTTTG GAATTAATCG CTGGCTGGTG TGAACACTTA 
GCTCAAAATA CATGGGGTGA TAGTACTATA TCTTTGGAAT ATCCTGGCAT ATATGAGCTT 
GTCAAATTAC CTCACAGGCT AGAAAATTTA ATTGATTCGA TGCAAGAATC AGTTTGCTGT 
ATGTGCCATA AAACTCCAAT CCTTCCTGCA ATTTGTATGT TATGTGGCAG TGTCATCTGT 
TTTAATGCTC GACAGAATAC GGTTTCCAGT AGGAGACTAA CTGGGGAATG CAACAAGCAT 
GCCGCTACGT GCACCGGTAG TGTCGGCATC TTTTTCATCA CCAAAGCATG TGGGATTTTG 
TTGTTAGACT CGATCAGTAA TACAGGCACT ATCATGCCCA CTCCGTACTT AGATATTCAT 
GGTGAAACCG ATTTGCAGCT TAGACGTGGT TGTCCTCAAT TTTTGAATCA AAAACGTTAT 
GATTTTGTTG TCCGTGAACA ATGGCTGCGC CAAACGGTCT TACAAAAAAT GGCTCGTCAT 
ATGGATATGA CGGAAATGCA AAACTGGCGA ATGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594298.1
CDS	302..6460
Translation	MQLHDPPAPLSQPSASRLQKYLLESAEKHAYDSNEESKTHLLQEVFLSLLNYNEDNWKYFLKEKPGAITSDFRLSRLQHSEPECAQELQDKRSGSKVCGHVFRAGEVIYRCKNCGLDNTCVLCAPCFHATNHEGHETHVSISTSYSGICDCGDPEAWNVDLNCKIHNVPDDEEQKKPEEVIPLELQHSIRTTIHILLDFILDVFSCSPVNLKAQSTVGSILADEEASRLSSAKYGVADRPCNVFRVMLWNDEVHTFDAVVGSVLEALDSSNTAFGLEVAQRVDSIGRFAVATSASVHEAIRIANAISKENLAVNVRTARDFFREDICGILLEWFDDLLESHVCYFADYLQIIVCDEILKNWSPGLEKPAKPEVNFNNLPLEIVNDDDSEDDIYAAEELLDVIANLQDETGVTRIANLGGDEDFEADMTDPTIAGFDHPLDDDNDVNDLLDFETEREDIDDLTDEVMETEENEAAEADYPGVNRNTRQDDVQDISMETESQNETDESQNTENVDYNPQTHTPVPIPTTATQDVVTIRPEFNSQLLNNLRQIINARRRPRPAAVCQVSLREDYWKSPHPIPPSSYSFVESPSSILRLDYFLLFDLKFWKRLRGLLSKLYVVPFNRNLLFKRLMGIRFVIHYRSLATAFLFADREPDHSVMFLSVQFFTTPSLAEAVVKDYDFLTNLNATTLSLLTQSNRPSTLFSSDIEYTPTIQLNRQVLKTRRTYNLFSDLGYLLQHPQVKKLVVDDTRYVHQYIDLLRVFQGVIPQQRAILSHVQWDFPHGKNILFVMQRVAMLSNTVSSCFTQAPYERLFYAIKCIITSITHPKLDIAESLEPLSCIPSSSLTNFTQPLVPFSVSRDPISFYHPLHWMLSNLFSYCRVDASSHWDKDTLLALLDHPLRVCVLLAQIDCNLWIRNGRSILLTDAFYRQLNNIEVSYDKDILAIQTILMFVDPNLVLNAVVQRFEFTDWLYNLTYNEHPNYDTERIPAMLCKMLELLIALITEREQILHVDIQDIIRTRLAQQLCFGPLAYSALLSTISSNLVESLSFDKIREEVTSYKAPDGLHDFGVYSLKDEYYDLVDPYYFHYNKNEREESDTILKKRLAKKNNVSAESIIIEPKIRFLEKDGHDIFFAAVNASTFSLIIFRAIEYALVQAESFGSSDIGNTILGDALQLCLISMKIHEFSKSNDFCSRSCAERYPTDSSIMREFGGSAYCLAELCFAILKSPKYKDVHVKVNAVLAGLQKNDPSAYSNMLEATHFELSTTSSTSDSNEIEKTQEKKRLALEKQKKIMQQFRDQQASFLAQNTDFDIGEDQTEDEVTTEEPEEEVKYHEHIRGNCLLCQEECNDQAPYGVIGIIQGSSLLRKTDVHSEIILDEIYSVPPNLDRESHSRPFGKKYDTVVFNRSKDRLLSAYPPGNNIRGVFVSGCGHLMHLGCFKNYYVARSMYRNDVTAGLSEYYYKYSTAKFFMCPLCRSLSNVLLPMPQIPKMCLNIDTLNFPRSMNGWLEEIGTMSSSSFEYQLVRSSLSDTKDTFRSCFLRPWINSKIISAMLARLKIADGALIDQSNNRDVSDLYDRYCETTKLAMKLVKGSTFTNVSPHDLLNSLAYTVSSLEVSQRCSPKQSGATRSVWFNELGPLTLSFLPTLSDTVLKCVCDQIIKSDQQALLLMESQKLLVCKIFYRHSQLKSMLRNGRMSDHDQIQPFLLSNTFDDFVKISSLMLIFGKQDNILYYVKLFYLSEICKTIISMIKVVADSSVVPDLTINYSQQSKSQFYILCKNVLLWCGSSNNIEILDDESNLLRLMSLVEKYSLPFLRRVALVLYCMFDISLEFNEFSNNEDDSELERLSKLIKVPPLQELYSQMSSDENNQILELIAGWCEHLAQNTWGDSTISLEYPGIYELVKLPHRLENLIDSMQESVCCMCHKTPILPAICMLCGSVICFNARQNTVSSRRLTGECNKHAATCTGSVGIFFITKACGILLLDSISNTGTIMPTPYLDIHGETDLQLRRGCPQFLNQKRYDFVVREQWLRQTVLQKMARHMDMTEMQNWRMA

Target ORF information:

RefSeq Version	NM_001019721.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative N-end-recognizing protein (ubr11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019721.2

ORF Insert Sequence:

ATGCAATTGC ACGACCCGCC GGCTCCTCTT TCGCAACCAT CGGCTTCTAG GTTACAAAAA 
TACCTTCTAG AAAGCGCGGA AAAGCATGCA TACGATTCAA ATGAAGAATC AAAAACGCAC 
TTGCTGCAAG AGGTTTTCCT GTCGTTGCTG AATTACAACG AAGACAATTG GAAATATTTT 
CTCAAAGAAA AGCCAGGTGC CATTACTTCT GACTTTCGTC TCTCGAGGCT TCAGCACTCT 
GAACCCGAAT GCGCCCAAGA ATTACAAGAT AAACGTTCTG GGTCCAAGGT TTGTGGCCAC 
GTATTTCGTG CCGGCGAGGT GATATATCGT TGCAAGAACT GTGGGCTAGA TAACACATGT 
GTTCTTTGTG CTCCTTGCTT TCATGCCACA AACCACGAGG GTCACGAGAC TCATGTATCT 
ATTTCAACCT CTTATAGTGG AATCTGCGAC TGTGGCGATC CTGAGGCATG GAATGTCGAC 
CTTAACTGCA AGATACATAA TGTCCCCGAT GACGAGGAGC AGAAAAAGCC AGAAGAAGTG 
ATCCCACTAG AACTTCAACA CTCTATCCGT ACAACTATTC ATATTCTTCT TGACTTCATT 
CTCGATGTCT TTTCTTGTTC CCCCGTGAAT CTTAAAGCTC AATCTACTGT CGGTAGCATC 
TTAGCAGATG AGGAGGCCTC TCGTCTTAGC AGCGCAAAGT ATGGTGTAGC TGATAGGCCT 
TGCAACGTTT TCCGTGTAAT GCTTTGGAAC GACGAAGTGC ATACCTTTGA CGCTGTTGTA 
GGTAGTGTGC TTGAGGCGTT AGATTCGTCA AATACGGCTT TTGGACTTGA AGTCGCTCAA 
CGTGTTGATT CTATTGGCCG CTTTGCTGTT GCTACATCGG CTTCAGTTCA CGAAGCAATA 
AGAATCGCGA ACGCTATTTC AAAAGAAAAC TTAGCTGTTA ATGTTCGTAC TGCCCGCGAT 
TTTTTTCGCG AAGATATTTG TGGTATTCTA TTAGAATGGT TCGATGATCT TCTAGAATCA 
CATGTTTGCT ATTTTGCTGA TTATTTACAA ATTATCGTTT GCGATGAAAT TTTGAAGAAC 
TGGTCTCCTG GCCTTGAAAA ACCTGCTAAG CCTGAGGTGA ATTTTAATAA CCTTCCTCTG 
GAGATTGTTA ATGACGATGA TAGCGAGGAT GATATTTATG CCGCAGAAGA ACTTCTCGAT 
GTTATCGCAA ACCTTCAAGA TGAAACAGGT GTAACTAGAA TTGCTAATTT GGGTGGTGAT 
GAAGATTTTG AAGCGGATAT GACCGATCCT ACTATTGCAG GATTTGATCA TCCACTTGAT 
GATGATAACG ACGTAAATGA CCTTCTGGAT TTTGAAACAG AACGAGAGGA TATTGATGAT 
TTAACTGATG AAGTTATGGA GACAGAAGAA AATGAAGCTG CTGAGGCTGA TTATCCTGGT 
GTAAACCGAA ACACTCGTCA AGACGATGTT CAAGATATTT CAATGGAAAC CGAAAGTCAA 
AACGAAACTG ATGAATCACA AAACACTGAA AATGTAGATT ATAATCCTCA GACGCATACT 
CCTGTACCTA TTCCCACTAC TGCAACTCAG GATGTCGTTA CTATTCGCCC GGAGTTTAAC 
TCTCAACTCC TTAATAACCT TCGACAAATC ATAAACGCTC GTCGTAGACC CCGCCCTGCT 
GCGGTTTGTC AAGTATCTCT TCGTGAGGAT TATTGGAAAA GTCCTCATCC AATTCCTCCT 
TCGTCATATA GCTTCGTTGA ATCTCCATCT TCCATTCTTC GTTTAGATTA CTTTTTGCTG 
TTTGATCTCA AATTTTGGAA ACGGTTAAGA GGGCTGCTCT CAAAATTGTA TGTTGTTCCT 
TTTAATAGAA ATCTTCTATT TAAGCGTCTT ATGGGTATAC GTTTCGTAAT CCATTATAGG 
TCTCTCGCTA CAGCGTTTCT TTTTGCAGAT AGAGAGCCAG ACCACTCTGT CATGTTTCTT 
TCCGTTCAAT TTTTCACTAC TCCTAGTCTT GCAGAAGCCG TTGTGAAAGA CTATGATTTT 
TTAACTAATT TAAACGCAAC AACTTTAAGC TTACTAACGC AGTCTAACCG GCCGTCAACC 
CTTTTTTCTT CAGACATTGA ATACACTCCC ACAATTCAGC TAAACCGACA AGTCTTAAAA 
ACCCGAAGGA CTTACAATTT GTTTAGTGAT CTCGGATACT TACTTCAACA TCCGCAAGTC 
AAAAAGCTTG TAGTGGATGA TACGCGTTAT GTTCATCAGT ATATTGATTT GTTACGGGTG 
TTCCAAGGTG TGATTCCACA ACAACGGGCA ATACTCAGTC ACGTACAGTG GGATTTTCCT 
CATGGGAAAA ATATATTGTT TGTAATGCAA AGGGTAGCTA TGCTCAGTAA CACTGTTTCA 
TCTTGTTTTA CACAAGCACC ATATGAACGG TTGTTTTATG CTATAAAATG TATCATAACT 
TCCATCACCC ACCCTAAATT AGACATTGCA GAGTCTCTCG AACCTCTCTC TTGTATTCCC 
TCTTCATCTT TGACAAATTT TACACAGCCC TTAGTTCCGT TTTCCGTGTC GAGGGATCCT 
ATAAGTTTTT ATCATCCTCT TCACTGGATG CTTTCCAATT TATTTTCATA TTGTCGTGTT 
GACGCTTCTT CTCATTGGGA TAAGGATACA CTACTTGCTC TTCTTGACCA TCCTTTGAGG 
GTTTGTGTGC TTTTAGCTCA AATTGATTGT AACCTTTGGA TAAGAAATGG GCGTAGTATT 
TTATTAACTG ACGCTTTCTA TCGTCAACTT AATAACATCG AAGTTTCTTA TGATAAGGAC 
ATCCTCGCTA TTCAAACAAT ATTAATGTTT GTTGATCCAA ACTTGGTTTT AAATGCAGTT 
GTTCAAAGAT TTGAATTTAC TGATTGGCTT TATAATTTGA CTTATAATGA ACATCCCAAT 
TACGACACGG AAAGAATACC AGCTATGTTG TGTAAAATGT TAGAGTTATT AATTGCCCTT 
ATAACAGAGC GCGAACAAAT TTTACATGTT GATATCCAAG ATATTATTCG CACTCGATTA 
GCTCAACAGC TATGCTTTGG TCCTCTTGCT TATTCTGCAT TGCTGTCTAC TATTTCAAGT 
AATTTAGTAG AGAGTTTGTC ATTTGATAAA ATTCGAGAAG AAGTAACTTC CTACAAAGCA 
CCTGACGGTC TCCACGATTT TGGCGTTTAC AGTTTGAAGG ACGAATATTA TGATTTGGTG 
GATCCATATT ATTTTCATTA TAATAAAAAT GAACGAGAGG AGTCAGATAC TATCCTTAAA 
AAACGTTTGG CGAAGAAAAA TAATGTAAGT GCTGAGTCGA TTATAATAGA ACCAAAAATT 
AGATTTCTTG AGAAGGACGG CCATGATATA TTTTTTGCAG CCGTCAATGC GAGCACTTTT 
AGTCTTATCA TTTTTCGTGC GATTGAGTAT GCACTTGTTC AAGCGGAGTC CTTTGGATCA 
TCCGATATTG GAAATACTAT TCTGGGCGAT GCACTTCAGT TGTGTCTAAT CTCTATGAAA 
ATTCATGAAT TCTCGAAATC AAATGACTTC TGCTCCCGTT CATGCGCTGA GCGTTATCCG 
ACTGATAGTA GTATTATGAG AGAATTTGGT GGCAGTGCGT ACTGCTTAGC GGAACTTTGT 
TTTGCAATTC TCAAGTCTCC TAAATACAAA GATGTTCATG TAAAAGTTAA TGCTGTATTG 
GCTGGCTTAC AAAAGAATGA TCCATCTGCA TATAGCAACA TGCTGGAAGC TACACACTTT 
GAACTCTCAA CTACCTCTTC TACTTCGGAT TCTAACGAGA TTGAGAAAAC GCAGGAAAAG 
AAAAGATTAG CTTTAGAGAA ACAGAAGAAA ATTATGCAGC AGTTTCGCGA TCAGCAAGCA 
TCTTTTCTTG CCCAAAATAC CGATTTCGAT ATCGGAGAAG ATCAAACGGA AGATGAAGTT 
ACAACAGAAG AGCCAGAGGA GGAAGTTAAG TACCATGAAC ATATCCGTGG TAATTGTTTG 
CTTTGCCAAG AGGAATGCAA CGATCAAGCA CCATATGGAG TGATCGGCAT TATTCAAGGA 
AGCTCATTAC TTCGAAAAAC AGATGTTCAC AGCGAAATCA TTTTGGATGA AATTTATAGT 
GTTCCCCCTA ATTTAGATAG GGAATCTCAT TCGAGGCCAT TTGGAAAAAA ATATGATACA 
GTTGTTTTCA ACCGTTCAAA AGATCGTTTA TTAAGTGCTT ATCCCCCAGG AAATAACATA 
AGAGGTGTTT TTGTCTCCGG TTGTGGTCAT CTCATGCATT TAGGTTGTTT TAAAAACTAT 
TACGTTGCTA GGTCCATGTA TAGAAATGAT GTTACCGCTG GGTTAAGTGA ATACTATTAC 
AAGTATTCTA CTGCTAAATT TTTTATGTGT CCTCTCTGTA GGTCATTGTC GAATGTGTTA 
TTGCCTATGC CTCAGATCCC CAAAATGTGT CTCAACATTG ACACCCTTAA TTTCCCTCGT 
AGCATGAATG GTTGGTTAGA GGAAATCGGT ACTATGTCTT CCTCGAGCTT TGAATATCAA 
TTAGTTCGAA GTTCTCTGTC CGACACGAAA GATACTTTTA GAAGCTGTTT CCTGAGACCT 
TGGATAAACA GCAAAATTAT CTCGGCAATG CTTGCTCGCT TAAAGATAGC CGATGGTGCA 
TTAATTGATC AAAGTAATAA TCGGGACGTA TCCGACCTTT ATGATCGATA TTGTGAAACA 
ACAAAATTGG CTATGAAATT GGTTAAAGGA AGTACATTTA CGAATGTTTC ACCTCATGAT 
TTACTGAACA GTTTGGCCTA TACCGTTTCT TCTCTAGAAG TTAGTCAGAG GTGCTCTCCG 
AAACAAAGTG GAGCAACTCG ATCGGTGTGG TTCAATGAAC TGGGACCCCT AACATTATCG 
TTTTTGCCGA CTTTATCTGA TACCGTTTTG AAGTGTGTTT GTGATCAGAT CATAAAATCT 
GACCAACAAG CCCTCTTGTT AATGGAGAGC CAAAAACTGT TAGTTTGTAA AATTTTCTAT 
CGTCACTCGC AATTAAAGTC CATGTTGAGA AATGGCCGGA TGTCTGACCA TGATCAAATT 
CAACCGTTTT TGCTTTCAAA TACTTTCGAT GACTTTGTGA AAATTTCATC TTTAATGCTA 
ATCTTTGGTA AACAAGATAA TATTTTGTAT TACGTTAAGC TTTTCTATCT TTCAGAAATT 
TGCAAGACAA TAATTTCTAT GATAAAAGTG GTTGCAGATT CATCGGTCGT TCCTGATTTA 
ACCATTAATT ATAGTCAGCA ATCCAAGTCC CAATTTTATA TTCTATGCAA GAATGTACTG 
CTTTGGTGTG GTTCTTCAAA TAACATTGAA ATTTTGGATG ATGAGTCTAA TTTACTTCGT 
TTAATGTCTT TGGTGGAGAA GTATTCTTTA CCGTTTCTCC GACGAGTTGC TTTGGTATTA 
TATTGCATGT TTGACATTTC ACTCGAGTTC AATGAATTCT CCAACAATGA AGATGACAGT 
GAATTAGAAA GGCTTTCCAA ATTAATAAAA GTTCCTCCTT TACAAGAACT GTATTCTCAA 
ATGTCTTCTG ATGAAAATAA TCAGATTTTG GAATTAATCG CTGGCTGGTG TGAACACTTA 
GCTCAAAATA CATGGGGTGA TAGTACTATA TCTTTGGAAT ATCCTGGCAT ATATGAGCTT 
GTCAAATTAC CTCACAGGCT AGAAAATTTA ATTGATTCGA TGCAAGAATC AGTTTGCTGT 
ATGTGCCATA AAACTCCAAT CCTTCCTGCA ATTTGTATGT TATGTGGCAG TGTCATCTGT 
TTTAATGCTC GACAGAATAC GGTTTCCAGT AGGAGACTAA CTGGGGAATG CAACAAGCAT 
GCCGCTACGT GCACCGGTAG TGTCGGCATC TTTTTCATCA CCAAAGCATG TGGGATTTTG 
TTGTTAGACT CGATCAGTAA TACAGGCACT ATCATGCCCA CTCCGTACTT AGATATTCAT 
GGTGAAACCG ATTTGCAGCT TAGACGTGGT TGTCCTCAAT TTTTGAATCA AAAACGTTAT 
GATTTTGTTG TCCGTGAACA ATGGCTGCGC CAAACGGTCT TACAAAAAAT GGCTCGTCAT 
ATGGATATGA CGGAAATGCA AAACTGGCGA ATGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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ubr11 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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