ORF » Species Summary » Schizosaccharomyces pombe » leu3 cDNA ORF clone

leu3 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following leu3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the leu3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100071 NM_001022019.3
Latest version!		 Schizosaccharomyces pombe 2-isopropylmalate synthase Leu3 (leu3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    leu3 ( NM_001022019.3 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596103.2 Schizosaccharomyces pombe 2-isopropylmalate synthase Leu3 (leu3), mRNA.
    Price & Availability↑
    CloneID OSc100071
    Clone ID Related Accession (Same CDS sequence) NM_001022019.3
    Accession Version NM_001022019.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product 2-isopropylmalate synthase Leu3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022019.2. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAAATCTA CTTTTGAGGC TGCTGGCCGC GTTGCCAAAG GGATGCTCAA GGATCCCTCC 
    AAAAAGTATA AGCCATTTAA AGGAATTCAA CTACCCAACC GTCAATGGCC AAACAAGGTT 
    TTGACGAAAG CTCCACGCTG GCTTTCTACG GACTTGCGTG ATGGTAATCA GGCTTTACCC 
    GATCCTATGA ATGGGCAGGA GAAATTGAGA TATTTTAAAT TGCTTTGCAG TATTGGCTTC 
    AAAGAAATTG AGGTTGGTTT CCCAAGTGCT TCTCAAACTG ATTTTGCATT TGTTCGTCAT 
    CTGATTGAAA CGCCAGGTTT GATTCCTGAC GATGTTACTA TTTCTGCCCT TACTCCTTCT 
    CGTGAGCCTT TGATCCTACG TACGATTGAG GCTCTTCGAG GCGCTAAGAA TGCCACTGTT 
    CACTTGTATA ATGCCTGTTC TCCTCTTTTC CGTGAAGTTG TCTTCCGCAA CAGTAAGCAA 
    GAAACATTGG ATTTAGCCAT CAAAGGCTCA AAAATCGTAA CAGCTGCTAC GAAAAATGCT 
    CTTGAATCGA AGGAAACCAA CTGGGGATTT GAATATTCTC CTGAAACTTT TTCAGACACC 
    GAACCAGACT TTGCTTTGGA AGTTTGTGAA GCTGTCAAGG GTATGTGGAA ACCTTCTGCT 
    GCCCAACCTA TTATCTTCAA TCTTCCTGCC ACTGTCGAAA TGTCTACGCC CAACACATAT 
    GCTGACTTAA TTGAGTACTT TTCCACTAAC ATTAGTGAAC GTGAAAAAGT CTGTGTTTCT 
    CTCCATCCCC ATAACGACCG TGGTACTGCT GTCGCAGCAG CTGAACTTGG TCAACTTGCC 
    GGAGGTGACC GTATTGAGGG CTGTTTGTTT GGCAATGGTG AACGTACTGG TAATGTAGAC 
    TTGGTTACTT TGGCTTTCAA CTTGTATACC CAAGGTGTTT CTCCTAACCT CGATTTCTCC 
    AAGTTGGATG AAATCATTCG TATTACTGAA GACTGTAACA AGATAAACGT TCATCCCCGT 
    CATCCTTATG CTGGCAATCT TGTCTTTACC GCCTTTTCTG GTTCTCATCA AGATGCCATT 
    TCTAAGGGTT TGAAGGCTTA CGATGAGCGT AAAGCTGTCG ATCCTGTTTG GAAAGTCCCT 
    TACTTGCCTT TGGATCCCCA TGATGTCAAT TCCGAGTATG CTGCTATTAT CCGCGTTAAC 
    TCTCAATCTG GCAAGGGTGG TGTCGCATAT CTGTTGAAGA CCAACTGTGG TCTCGATTTA 
    CCTCGTGCTT TGCAAGTTGA ATTTGGTAGT ATTGTTAAGG ATTATAGCGA CACAAAAGGA 
    AAGGAGCTTA GCATTGGTGA GATCAGCGAC CTGTTTTATA CCACATATTA CCTCGAATTT 
    CCCGGCCGTT TCTCTGTAAA CGACTACACT CTTTCTAGCA ACGGACCTCA AAGCAAATGT 
    ATTAAATGCG TTGTTGACAT CAAGGGTGAA AAGAAAGATA CTCCTTCGCG GGTTGTGATC 
    GAGGGTGTTG GAAATGGACC TTTGTCGGCA TTGGTTGATG CTTTACGCCG TCAGTTCAAT 
    ATTTCATTTG ACATTGGTCA ATACTCTGAA CATGCTATTG GTTCTGGTAA CGGCGTCAAA 
    GCTGCTTCTT ATGTTGAGAT CATTTTCAAT AACACTTCTT TCTGGGGTGT TGGTATTGAT 
    GCTGACGTTA CCTCTGCCGG ATTAAAGGCT GTCATGTCAG GCGTTAGTCG TGCCTCCCGC 
    GCATTTGCTA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596103.2
    CDS342..2096
    Translation

    Target ORF information:

    RefSeq Version NM_001022019.3
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe 2-isopropylmalate synthase Leu3 (leu3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001022019.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAAATCTA CTTTTGAGGC TGCTGGCCGC GTTGCCAAAG GGATGCTCAA GGATCCCTCC 
    AAAAAGTATA AGCCATTTAA AGGAATTCAA CTACCCAACC GTCAATGGCC AAACAAGGTT 
    TTGACGAAAG CTCCACGCTG GCTTTCTACG GACTTGCGTG ATGGTAATCA GGCTTTACCC 
    GATCCTATGA ATGGGCAGGA GAAATTGAGA TATTTTAAAT TGCTTTGCAG TATTGGCTTC 
    AAAGAAATTG AGGTTGGTTT CCCAAGTGCT TCTCAAACTG ATTTTGCATT TGTTCGTCAT 
    CTGATTGAAA CGCCAGGTTT GATTCCTGAC GATGTTACTA TTTCTGCCCT TACTCCTTCT 
    CGTGAGCCTT TGATCCTACG TACGATTGAG GCTCTTCGAG GCGCTAAGAA TGCCACTGTT 
    CACTTGTATA ATGCCTGTTC TCCTCTTTTC CGTGAAGTTG TCTTCCGCAA CAGTAAGCAA 
    GAAACATTGG ATTTAGCCAT CAAAGGCTCA AAAATCGTAA CAGCTGCTAC GAAAAATGCT 
    CTTGAATCGA AGGAAACCAA CTGGGGATTT GAATATTCTC CTGAAACTTT TTCAGACACC 
    GAACCAGACT TTGCTTTGGA AGTTTGTGAA GCTGTCAAGG GTATGTGGAA ACCTTCTGCT 
    GCCCAACCTA TTATCTTCAA TCTTCCTGCC ACTGTCGAAA TGTCTACGCC CAACACATAT 
    GCTGACTTAA TTGAGTACTT TTCCACTAAC ATTAGTGAAC GTGAAAAAGT CTGTGTTTCT 
    CTCCATCCCC ATAACGACCG TGGTACTGCT GTCGCAGCAG CTGAACTTGG TCAACTTGCC 
    GGAGGTGACC GTATTGAGGG CTGTTTGTTT GGCAATGGTG AACGTACTGG TAATGTAGAC 
    TTGGTTACTT TGGCTTTCAA CTTGTATACC CAAGGTGTTT CTCCTAACCT CGATTTCTCC 
    AAGTTGGATG AAATCATTCG TATTACTGAA GACTGTAACA AGATAAACGT TCATCCCCGT 
    CATCCTTATG CTGGCAATCT TGTCTTTACC GCCTTTTCTG GTTCTCATCA AGATGCCATT 
    TCTAAGGGTT TGAAGGCTTA CGATGAGCGT AAAGCTGTCG ATCCTGTTTG GAAAGTCCCT 
    TACTTGCCTT TGGATCCCCA TGATGTCAAT TCCGAGTATG CTGCTATTAT CCGCGTTAAC 
    TCTCAATCTG GCAAGGGTGG TGTCGCATAT CTGTTGAAGA CCAACTGTGG TCTCGATTTA 
    CCTCGTGCTT TGCAAGTTGA ATTTGGTAGT ATTGTTAAGG ATTATAGCGA CACAAAAGGA 
    AAGGAGCTTA GCATTGGTGA GATCAGCGAC CTGTTTTATA CCACATATTA CCTCGAATTT 
    CCCGGCCGTT TCTCTGTAAA CGACTACACT CTTTCTAGCA ACGGACCTCA AAGCAAATGT 
    ATTAAATGCG TTGTTGACAT CAAGGGTGAA AAGAAAGATA CTCCTTCGCG GGTTGTGATC 
    GAGGGTGTTG GAAATGGACC TTTGTCGGCA TTGGTTGATG CTTTACGCCG TCAGTTCAAT 
    ATTTCATTTG ACATTGGTCA ATACTCTGAA CATGCTATTG GTTCTGGTAA CGGCGTCAAA 
    GCTGCTTCTT ATGTTGAGAT CATTTTCAAT AACACTTCTT TCTGGGGTGT TGGTATTGAT 
    GCTGACGTTA CCTCTGCCGG ATTAAAGGCT GTCATGTCAG GCGTTAGTCG TGCCTCCCGC 
    GCATTTGCTA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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