tor1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	tor1
Entrez Gene ID	2540473
Full Name	phosphatidylinositol kinase Tor1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022196.2	NP_596275.1	phosphatidylinositol kinase Tor1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Since Gaf1 was also critical for transcription of per1+ and put4+, Tor-Gaf1 signaling may coordinate transcription of multiple amino acid permeases according to nutrient availability
Title: Tor Signaling Regulates Transcription of Amino Acid Permeases through a GATA Transcription Factor Gaf1 in Fission Yeast.

The Gad8 kinase (AKT homologue) phosphorylates fission yeast Tor1 at threonine (T1972) to reduce activity.
Title: Phosphorylation of the TOR ATP binding domain by AGC kinase constitutes a novel mode of TOR inhibition.

While mutated TORC1 and TORC2 displayed diminished kinase activity and FKBP12/Fkh1-dependent rapamycin sensitivity, their phenotypes were nearly opposite in mitosis.
Title: The reverse, but coordinated, roles of Tor2 (TORC1) and Tor1 (TORC2) kinases for growth, cell cycle and separase-mediated mitosis in Schizosaccharomyces pombe.

Data highlight critical roles for TORC2 and its component Tor1 in chromatin metabolism and in promoting mitotic entry, most notably after recovery from DNA-damaging conditions.
Title: TOR complex 2 controls gene silencing, telomere length maintenance, and survival under DNA-damaging conditions.

Results suggest that S. pombe Tor1 and Tor2 can both act in TORC1, but it is the inhibition of Tor1 as part of TORC1 that promotes mitosis following nutrient stress.
Title: Fission yeast Tor1 functions as part of TORC1 to control mitotic entry through the stress MAPK pathway following nutrient stress.

The following tor1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tor1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100283	NM_001022196.2 Latest version!	Schizosaccharomyces pombe phosphatidylinositol kinase Tor1 (tor1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tor1 ( NM_001022196.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596275.1 Schizosaccharomyces pombe phosphatidylinositol kinase Tor1 (tor1), mRNA.

Price & Availability↑

CloneID	OSc100283
Clone ID Related Accession (Same CDS sequence)	NM_001022196.2
Accession Version	NM_001022196.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	phosphatidylinositol kinase Tor1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022196.1.

ATGGAGTATT TTAGTGATCT AAAAAACAAA AATGAAAGCA TTCAACTAGC AGCCGCTGAT 
CAATTAAAAG AATTTGTACA TAGTTCTACT AAGGAATTAT CAGGTGAATC CCTTGCGCGT 
TTCAATAACG ATATCAATCG TCGAATTTTT GAGCTTATTC ATAGCCATGA TTCCCACGAA 
AGATTTGGAG GAATCCTTGC GATAGGCAAG CTAATTGAAT TCGAAAGTGA GGGAGATGTT 
ACTAATCTTT CGCGGTATGC AAATTACTTA CGAATGACCC TTCCTAGTAC GGATTGGCAT 
TCGATGGAAC TATCTGCGAA GGTTCTTGGT CATTTGGCTG CGTCTGGTGG TACCCTTGCA 
GCCGAATTCG TTGAATTTGA AGTTCAGCGT GCTTTCGAGT GGCTTCAAGG AGACCGTCAA 
GAGCAAAAAC GGATGGCGGC TATTTTAATT ATTAAGGCTT TAGCACAAAA TTCTCCTACC 
TTAGTTTACT TGTATATTAG TGAAATTTTC CAAAATTTGT GGACTGGATT GCGTGATCCA 
AAGCCTCTTA TTCGGGAAAC TGCTGCTGAT GCTTTGGGTG CTTCTTTAGA CGTGGTTTGT 
CAAAGAGAAG CTAAAGTTCA ATTGCAGTGT TTTAATGAAG TTCTGCTTCA AGCTGAACAT 
GGTTTACGAC AGTCGAGTGT GGAATATCTG CATGGAAGTC TTCTCGCATA CAAAGAGCTT 
TTCGAAAAAT CTGGCTCTTT TATTCGTGAA CATTACACAG AATTTTGTGA TTTGGCGCTT 
CGTTTACGAG AGCATAGAGA TAACTCTATC AGACGCTGCA TAGTATTTTT ACTGCCTACA 
TTGTCTGAAT ATAACCCCAA AAAATTTCAA CAGCGTTATT TAGATTCTTT TATGGTTTAC 
TTACTTTCCC ATATCAGGAA AGACAAGGAA AAGTCTTTAG CTTTTGAAGC TATTGGAAGA 
ATAGCCATGG CAGTCAATGA AGCAATGATT CCTTATTTAC AGAATATTTT AAAAGTAATA 
AGGGATACAT TGACAGCAAA AGTACGTGAA AAAACGCAAT ATGAAAAGCC TGTTTTTGAA 
TGCATTGGCA TGTTGGCTGC TGCTGTAAAG CTTGAACTCC TTGAAGATTC TCGAAGTCTA 
CTTGGTCTGA TTTTTTCTTG CGAACTGAGC GTTCACTTGC GTCAAGCTCT TGTGAAAATG 
GCAGAAAACA TCCCTCCTCT TTTGGCTCCT ATTCAAGAAC GATTATTGAA TATGGTTAGT 
CAAATTTTGA CTGGAAAAAA CTTTGAAATT CGTACTAATG ACACATATAC TCCCAGTTTC 
ACTAATATAT ACTCTGCTAG GGAACCTGAC CAAAGAAGTA AAAGCACGGA ATCAATTATT 
TTGGCTTTAG AAACTTTGGG TACTTTTAAT TTTACTGGTT ATTCATTAAT TAGTTTCATC 
CAAGAATCTG TCTTATCTTA CTTGGAAAAT GATAATTCAG AGATCCGTAT TGCTGCTGCA 
CGAACTTGCT GTCAGGTTTT TGCCAGAGAT CCGATATGTA GAAAAACAAA TCCTTTGGCT 
GTTGAATCAG TCGCTGAGGT GCTTGAAAAG CTATTAACCC TTGGAATTGC AGATAGTGAT 
CCAAAAATTA GGGAAACCGT ACTCTCATTA CTAGATGAAA GATTTGATCG GCATTTGGCA 
CATCCTGATA ATATTCGTTG CTTATTTATC GCTCTGAATG ATGAAGTTTT TTCAATCAGA 
GAAATTGCTA TCATTATTAT AGGAAGGCTT GCATTGTATA ATCCGGCCCA TGTGATGCCA 
TCGCTACGAA AAACTATTAT TCAGTTATTA TCTGACATGG AATATTCGGG TAATAGTCGT 
CAAAAAGAAG AAAGCGCACA GCTTCTCAAA TTACTGGTAT CCAAAGCTAG AACACTCATT 
AAGCCATATA TCCAATCGAT CATTCATGTA ATATTACCGA AAGCAGCAGA TACGAGTCCT 
GGAGTTTCAT CTGCCATTAT ATCAGCTTTA GGTGAACTCG CAAGTGTTGA AGGAGAAGAT 
ATGCCTGTGG ATGTTCGTGG TTCCTTCATG AAGTTAATAC TTGTGAATTT ACAGGATCAA 
AGCTCTACAT TGAAGCGACT TGCTTCTCTT AAATGTTTAC GAAAACTTTG TGGTCGTTCA 
GGTTATGTAA TTCAGCCATA TTTAGATTAC CCTCCTTTGT TGGGCGCGCT AATTGGTATT 
TTGCAATCAG AACAACCAAC ACCGATAAGA AGAGAAGTAC TGAGAACATT AGGTGTGTTA 
GGTGCTTTAG ATCCATATAC GTATTTAACT ACTGAGGAAG TTTCAGATGA TTTACAAAGT 
AGTCATAATA ATGCCCATGG TGTACCTCAA ATTAGTGCCG CGCAGTACCC GTCATTGGAA 
AACTACGCTA TGGTTGCTGT TGTCACTTTG ATAGGTATTT TAAAAGACAG TTCGCTCTCT 
ATGCATCACT CTTCTGTTGT TCAAGCAGTG ATGCATATTT GCTCTCAAAT GGGATCTAAA 
TCTACTGTAT TTTTACCTCA AGTGGTACCA ACGTTTCTAC AAGTAATGCA ATCCTTATCG 
GCTTCCTCGG CTGAGTTTTA TTTTCAGCAG TTAACGACTT TAACTTCTAT TATTGGACCA 
AACATTAGGG ATTATGTATC TGATATTTTT AATCTTTCAA AAGTCTTTTG GGAATCTACC 
ACATCTCTAT TGCTAGTTAT TCTTGAACTC ATCGATGCCA TAGCAATTGC ACTGCAGGAT 
GAATTTAAAT TTTACTTGCC TCAAATATTA TCTTGTATGT TGAAAGCATT TTCCCTTGAT 
AATACGTCTT CCCGTTCCGT TTCATACAAA GTTTTACAAT CATTTGTTAT ATTTGGTTCT 
AATATTGAAG AATACATGCA TCTTGTTCTA CCAGTTATAA TTAGAAGTTT TGAGCGTGAT 
ACTATACCCC TTGGATTCAG GAAGAGTGCC CTCAAATGCA TTGCCCAATT ATTTCAATCT 
GTTAATTTCT CGGATCATGC CTCTCGAATC ATTCATCCAT TGGTTCGCAT GCTAGGGAAA 
TCTAATGGCG ATTTGAGAGC AGTTATTATG GATACTCTTT GTGCTATAGT ATCGCAACTT 
GGGTATGATT ATTCGATTTT TATACCGATG GTGAATAAAG TTTTGGTTAG TCACAAGATA 
AGTCATCCGG CTTACGAACT TTTGGTTTCA CGACTTTTGA AAGGTGAGCC TCTACCCAAA 
GATGTCGTAG TAAAGGAGTT TAAGCCGAGG CCCTCAACAA AGCCTTTTTC TACACAAGAT 
GAAGTCTTGA CAAAGCTTCC TGTTGATCAA GCATCCTTGA AAGCAGCATG GGAATCATCA 
CAAAAATTGA CAAGGGATGA TTGGCAGGAC TGGATAAGAA GAATAAGTAT TGAACTTCTG 
AAGGAATCCC CTTCTTCGGC ACTTAGGTCT TGTTCGACCT TAGCTGGTAT ATACCATCCA 
TTGGCTCGCG ATTTATTTAA TGTCAGTTTT TTGTCTTGTT GGGATGAGTT AACTGAAAGC 
AATAAGAAAA ATCTCGTTAA ATCAATAGAG CTCGCTATGA ATGCCCCTAA CATTTCAGTA 
GAAATATTAC AGACTCTGTT AAATCTTGCT GAGTATATGG AGCGTGAAGA TCATACATTA 
CCAATTCCTA TCAAAGTAAT TAGTGCTCAT GCATCTAAGT GTAATGTTTA TGCCAAGGCT 
CTGCATTATA CAGAATTACA ATTCGTTCAA GAGACGAAAG AGGAAGTTAG CATTAGCACT 
ATCGAGTCTC TCATTACCAT AAACAATCAT CTGCAACAAT CCGATGCCGC TGTAGGTATG 
CTTCAATATA CCAAAGAACA CAAGCAGTTT AGTTTGAAAG AAACTTGGTA TGAAAAATTA 
CATAGATGGG ATGATGCATT AGCTGCATAT GAGCATCGAG AACGTGAAGG TGACTCGTCT 
TTCGAGATAA ATATCGGAAA ATTACGCTGT TATTATGCTT TGGGTGACTG GGATCACCTT 
TCCGAATTGG CACAAAAAGC TTGGGTAACG TCTGAACAAG AACATCGTGA AGCTATTGCT 
CCACTTGCAG CTGCCGCTGC TTGGGGTTTG GGTCAATGGA ATTTAATTAG TGAATATGTT 
AGTGCCATGG ACCGTGACCC ACAAGATAAA GAGTTTTTTT CCGCTATTAG TGCTGTACAT 
CTTGGTCAAT ACAATAAGGC GTATGGCCAT ATCGAGAGAC ATCGTGATAT ATTAGTAAAC 
GACCTTTCTT CCATAATCGG TGAAAGCTAT AATCGTGCAT ATGGTATTAT GGTCAAATCC 
CAAATGCTTT CGGAACTTGA AGAAATTATT GACTATAAAA AGAATATGCA ATATGAAAAC 
AATTTGGACT CTCTTAAAAA AACGTGGCGG AAAAGGCTTG AAGGATGTCA GAAAAATGTG 
GATGTTTGGC ATAATACGCT TCGGTTTAGG GCTCTGGTAT TGTCTCCACA AGATTCTCCT 
GAAATGTGGA TAAAATTAGC TGATCTTTGC CGTCGTTCTG ACCGGTTGAA GCTTTCAAAT 
CAGTGTCTCA CATATTTGAT GGGTCGCGAT CCTTCAAATG CTTACCCGCT TGACTCACTT 
AAGCTTCTCA ATCCTCATGT TGTATATACG TATTTAAAAT ATTTGTGGGC TACAGATCAA 
AAAAATATTG CGGTATCGGA GCTCGAAGAG TTCACCTCAT ATCTATCGTC AAAACATGGG 
TATAAAATGG GTGATTCTTC AAAGTTGGTT GATATTTTAG CCTCATCATC TGTAAGTTCG 
GAAGAGCGTT CGTTTTTAGC AAGGTGTTTT CATAAACTTG GCAAATGGAA AAAGTCTCTT 
CAAGATAGTG TTAATCAAGA AAGCGTAAGG GATATTTTGA ATTGTTATTT TTATGCTACT 
TTGTTCGATA AATCGTGGTA TAAAGCTTGG CACTCTTGGG CTCTCGCAAA TTTCGAAGTC 
GTTGGTTATT ATGAACAGAC TGAACATGGG GTTACCCAAG ATATGTATGA ACAATATATT 
GTACCTGCTA TTAAAGGATT TTTTCACTCT TCTGTTTTAA ATCAAAAAAA CTCACTTCAG 
GATATATTGC GGTTACTTAA TTTATGGTTT AAATTCGGTG AGCACAGCGA CGTAGCAGCC 
GCAATTGTTG AAGGATTTTC CAATGTTCCA ATGGATACAT GGTTAGAAGT TATACCACAA 
CTAATTGCAC GAATACACAC TTCAAGTTCT TCAGTTAGAG CATCTGTTCA TCAGTTACTT 
TCCGATATTG GTAGGGTGCA TCCTCAGGCA TTGGTGTATT CACTTACTGT GTCCTCCAAA 
TCTACAAATC CGCAGCAGAA GCATTCAGCA AAATCAATTA TGGATAGCAT GCTTTCGCAT 
AGTGATACGT TGGTTCGTCA AGCTCTACTT GTTAGTCAAG AGCTAATTAG AGTTGCTATA 
CTTTGGCATG AATTATGGTA TGAAGGATTA GAGGAAGCTT CTCAAGCTTA TTTTTCAGAT 
CACGATATAT CTTTAATGAT TGATATTGTG AAACCCCTGC ATGAAACATT GGAGAAGGGT 
CCTTCCACCT TGTCTGAGAT ATCATTTGCT CAGACATTTG GGTATGATTT ACGTAAAGCT 
AGAAGTTACT GGCAGAAATT TTTGCAAGAT GGTGACCCTA CGGAATTAAA TCAGTCTTGG 
GATTTGTATT ATCAAGTCTT TAGACGTATT CAAAAGCAAT TACCACGGAT TAAGCATTTG 
GAGCTACAAT ATGTTTCCCC TAAGTTATTG GATGCTTGTG ATTTGGAACT AGCTGTGCCT 
GGAACCTATG GTCATAACAA ACCGGTCATT CGGATCTCGC ATTTCCATCA CACTTTCGAA 
GTTATTTCTT CTAAGCAAAG GCCAAGAAGA TTGACAATTC ATGGTAGTGA TGGCAAAGAT 
TATCAGTATG TCCTTAAGGG TCATGAAGAT TTGAGGCAAG ATGAACGAGT TATGCAGCTT 
TTTGGTTTAT GTAATACGTT ACTGACTACG GATTCTGAGA CATTCAAACG ACGTCTGAAT 
ATAGAGCGTT ATACAGTTAT TCCGCTATCT CCCAATTCTG GTTTGCTGGG GTGGGTTCCG 
CATAGTGATA CATTACATTT CCTGATCAAA GAGTTTCGCT CAAAGCGTAA CATATTGTTA 
AATTTAGAGC ATCGCATGAT GCTTCAAATG GCACCCGACT GCGACAGTTT AACATTATTG 
CAAAAACTAG AAGTATTCGA ATATGTTATG GCTAATACTG ATGGTTATGA TTTGTATCAT 
GTGTTGTGGC TTAAAAGTCG AAGTTCAGAA GCATGGCTTG ATAGGAGAAC CAGCTATACT 
CAAAGCTTGG CAGTCATGTC AATGGTTGGA TATATACTTG GACTTGGTGA CCGACATCCT 
TCCAATCTAA TGATGGATCG GTATTCTGGT AAGATCATTC ATATTGACTT TGGTGATTGT 
TTTGAGGTTG CCATGCATAG AGAAAAATTT CCTGAGAAAA TACCGTTTCG ACTTACTCGA 
ATGCTTATTA ATGCTATGGA GGTTAGCGGT ATCCAAGGTA CTTACAAAAT TACTTGCGAG 
CTTGTTATGC GTGTTTTAAG ATCAAATACG GAGTCTTTAA TGGCAGTTCT TGAAGCATTT 
GTCTATGACC CTTTGATTAA TTGGAGACTT ATGACTAAAA GCTCTTTCGG GGCTTCTACG 
ACACTCCGGC CTACCTCAAG TAGTGTAGAA GAGAAAGGAA GGTCCTACAC TCATCGTGCG 
CGTCACGCTG ATTATGCAGC TCTTAGTGAA ACAAATGGTG TAAACGCCGA AGGACTCAAC 
GAGAGATCTA TTCAAGTCTT GAAGCGTGTC TCAAATAAGC TAACTGGTAA AGATTTCGAT 
TTGAAAGAGC AATTGCCAGT CAAAGCGCAA GTTGAAAAGC TTATCCAACA AGCTACAGCT 
CCCGAAAATC TTTGCCGTTG TTATGTAGGA TGGTGTAGTT TTTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596275.1
CDS	200..7207
Translation	MEYFSDLKNKNESIQLAAADQLKEFVHSSTKELSGESLARFNNDINRRIFELIHSHDSHERFGGILAIGKLIEFESEGDVTNLSRYANYLRMTLPSTDWHSMELSAKVLGHLAASGGTLAAEFVEFEVQRAFEWLQGDRQEQKRMAAILIIKALAQNSPTLVYLYISEIFQNLWTGLRDPKPLIRETAADALGASLDVVCQREAKVQLQCFNEVLLQAEHGLRQSSVEYLHGSLLAYKELFEKSGSFIREHYTEFCDLALRLREHRDNSIRRCIVFLLPTLSEYNPKKFQQRYLDSFMVYLLSHIRKDKEKSLAFEAIGRIAMAVNEAMIPYLQNILKVIRDTLTAKVREKTQYEKPVFECIGMLAAAVKLELLEDSRSLLGLIFSCELSVHLRQALVKMAENIPPLLAPIQERLLNMVSQILTGKNFEIRTNDTYTPSFTNIYSAREPDQRSKSTESIILALETLGTFNFTGYSLISFIQESVLSYLENDNSEIRIAAARTCCQVFARDPICRKTNPLAVESVAEVLEKLLTLGIADSDPKIRETVLSLLDERFDRHLAHPDNIRCLFIALNDEVFSIREIAIIIIGRLALYNPAHVMPSLRKTIIQLLSDMEYSGNSRQKEESAQLLKLLVSKARTLIKPYIQSIIHVILPKAADTSPGVSSAIISALGELASVEGEDMPVDVRGSFMKLILVNLQDQSSTLKRLASLKCLRKLCGRSGYVIQPYLDYPPLLGALIGILQSEQPTPIRREVLRTLGVLGALDPYTYLTTEEVSDDLQSSHNNAHGVPQISAAQYPSLENYAMVAVVTLIGILKDSSLSMHHSSVVQAVMHICSQMGSKSTVFLPQVVPTFLQVMQSLSASSAEFYFQQLTTLTSIIGPNIRDYVSDIFNLSKVFWESTTSLLLVILELIDAIAIALQDEFKFYLPQILSCMLKAFSLDNTSSRSVSYKVLQSFVIFGSNIEEYMHLVLPVIIRSFERDTIPLGFRKSALKCIAQLFQSVNFSDHASRIIHPLVRMLGKSNGDLRAVIMDTLCAIVSQLGYDYSIFIPMVNKVLVSHKISHPAYELLVSRLLKGEPLPKDVVVKEFKPRPSTKPFSTQDEVLTKLPVDQASLKAAWESSQKLTRDDWQDWIRRISIELLKESPSSALRSCSTLAGIYHPLARDLFNVSFLSCWDELTESNKKNLVKSIELAMNAPNISVEILQTLLNLAEYMEREDHTLPIPIKVISAHASKCNVYAKALHYTELQFVQETKEEVSISTIESLITINNHLQQSDAAVGMLQYTKEHKQFSLKETWYEKLHRWDDALAAYEHREREGDSSFEINIGKLRCYYALGDWDHLSELAQKAWVTSEQEHREAIAPLAAAAAWGLGQWNLISEYVSAMDRDPQDKEFFSAISAVHLGQYNKAYGHIERHRDILVNDLSSIIGESYNRAYGIMVKSQMLSELEEIIDYKKNMQYENNLDSLKKTWRKRLEGCQKNVDVWHNTLRFRALVLSPQDSPEMWIKLADLCRRSDRLKLSNQCLTYLMGRDPSNAYPLDSLKLLNPHVVYTYLKYLWATDQKNIAVSELEEFTSYLSSKHGYKMGDSSKLVDILASSSVSSEERSFLARCFHKLGKWKKSLQDSVNQESVRDILNCYFYATLFDKSWYKAWHSWALANFEVVGYYEQTEHGVTQDMYEQYIVPAIKGFFHSSVLNQKNSLQDILRLLNLWFKFGEHSDVAAAIVEGFSNVPMDTWLEVIPQLIARIHTSSSSVRASVHQLLSDIGRVHPQALVYSLTVSSKSTNPQQKHSAKSIMDSMLSHSDTLVRQALLVSQELIRVAILWHELWYEGLEEASQAYFSDHDISLMIDIVKPLHETLEKGPSTLSEISFAQTFGYDLRKARSYWQKFLQDGDPTELNQSWDLYYQVFRRIQKQLPRIKHLELQYVSPKLLDACDLELAVPGTYGHNKPVIRISHFHHTFEVISSKQRPRRLTIHGSDGKDYQYVLKGHEDLRQDERVMQLFGLCNTLLTTDSETFKRRLNIERYTVIPLSPNSGLLGWVPHSDTLHFLIKEFRSKRNILLNLEHRMMLQMAPDCDSLTLLQKLEVFEYVMANTDGYDLYHVLWLKSRSSEAWLDRRTSYTQSLAVMSMVGYILGLGDRHPSNLMMDRYSGKIIHIDFGDCFEVAMHREKFPEKIPFRLTRMLINAMEVSGIQGTYKITCELVMRVLRSNTESLMAVLEAFVYDPLINWRLMTKSSFGASTTLRPTSSSVEEKGRSYTHRARHADYAALSETNGVNAEGLNERSIQVLKRVSNKLTGKDFDLKEQLPVKAQVEKLIQQATAPENLCRCYVGWCSFW

Target ORF information:

RefSeq Version	NM_001022196.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe phosphatidylinositol kinase Tor1 (tor1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022196.2

ORF Insert Sequence:

ATGGAGTATT TTAGTGATCT AAAAAACAAA AATGAAAGCA TTCAACTAGC AGCCGCTGAT 
CAATTAAAAG AATTTGTACA TAGTTCTACT AAGGAATTAT CAGGTGAATC CCTTGCGCGT 
TTCAATAACG ATATCAATCG TCGAATTTTT GAGCTTATTC ATAGCCATGA TTCCCACGAA 
AGATTTGGAG GAATCCTTGC GATAGGCAAG CTAATTGAAT TCGAAAGTGA GGGAGATGTT 
ACTAATCTTT CGCGGTATGC AAATTACTTA CGAATGACCC TTCCTAGTAC GGATTGGCAT 
TCGATGGAAC TATCTGCGAA GGTTCTTGGT CATTTGGCTG CGTCTGGTGG TACCCTTGCA 
GCCGAATTCG TTGAATTTGA AGTTCAGCGT GCTTTCGAGT GGCTTCAAGG AGACCGTCAA 
GAGCAAAAAC GGATGGCGGC TATTTTAATT ATTAAGGCTT TAGCACAAAA TTCTCCTACC 
TTAGTTTACT TGTATATTAG TGAAATTTTC CAAAATTTGT GGACTGGATT GCGTGATCCA 
AAGCCTCTTA TTCGGGAAAC TGCTGCTGAT GCTTTGGGTG CTTCTTTAGA CGTGGTTTGT 
CAAAGAGAAG CTAAAGTTCA ATTGCAGTGT TTTAATGAAG TTCTGCTTCA AGCTGAACAT 
GGTTTACGAC AGTCGAGTGT GGAATATCTG CATGGAAGTC TTCTCGCATA CAAAGAGCTT 
TTCGAAAAAT CTGGCTCTTT TATTCGTGAA CATTACACAG AATTTTGTGA TTTGGCGCTT 
CGTTTACGAG AGCATAGAGA TAACTCTATC AGACGCTGCA TAGTATTTTT ACTGCCTACA 
TTGTCTGAAT ATAACCCCAA AAAATTTCAA CAGCGTTATT TAGATTCTTT TATGGTTTAC 
TTACTTTCCC ATATCAGGAA AGACAAGGAA AAGTCTTTAG CTTTTGAAGC TATTGGAAGA 
ATAGCCATGG CAGTCAATGA AGCAATGATT CCTTATTTAC AGAATATTTT AAAAGTAATA 
AGGGATACAT TGACAGCAAA AGTACGTGAA AAAACGCAAT ATGAAAAGCC TGTTTTTGAA 
TGCATTGGCA TGTTGGCTGC TGCTGTAAAG CTTGAACTCC TTGAAGATTC TCGAAGTCTA 
CTTGGTCTGA TTTTTTCTTG CGAACTGAGC GTTCACTTGC GTCAAGCTCT TGTGAAAATG 
GCAGAAAACA TCCCTCCTCT TTTGGCTCCT ATTCAAGAAC GATTATTGAA TATGGTTAGT 
CAAATTTTGA CTGGAAAAAA CTTTGAAATT CGTACTAATG ACACATATAC TCCCAGTTTC 
ACTAATATAT ACTCTGCTAG GGAACCTGAC CAAAGAAGTA AAAGCACGGA ATCAATTATT 
TTGGCTTTAG AAACTTTGGG TACTTTTAAT TTTACTGGTT ATTCATTAAT TAGTTTCATC 
CAAGAATCTG TCTTATCTTA CTTGGAAAAT GATAATTCAG AGATCCGTAT TGCTGCTGCA 
CGAACTTGCT GTCAGGTTTT TGCCAGAGAT CCGATATGTA GAAAAACAAA TCCTTTGGCT 
GTTGAATCAG TCGCTGAGGT GCTTGAAAAG CTATTAACCC TTGGAATTGC AGATAGTGAT 
CCAAAAATTA GGGAAACCGT ACTCTCATTA CTAGATGAAA GATTTGATCG GCATTTGGCA 
CATCCTGATA ATATTCGTTG CTTATTTATC GCTCTGAATG ATGAAGTTTT TTCAATCAGA 
GAAATTGCTA TCATTATTAT AGGAAGGCTT GCATTGTATA ATCCGGCCCA TGTGATGCCA 
TCGCTACGAA AAACTATTAT TCAGTTATTA TCTGACATGG AATATTCGGG TAATAGTCGT 
CAAAAAGAAG AAAGCGCACA GCTTCTCAAA TTACTGGTAT CCAAAGCTAG AACACTCATT 
AAGCCATATA TCCAATCGAT CATTCATGTA ATATTACCGA AAGCAGCAGA TACGAGTCCT 
GGAGTTTCAT CTGCCATTAT ATCAGCTTTA GGTGAACTCG CAAGTGTTGA AGGAGAAGAT 
ATGCCTGTGG ATGTTCGTGG TTCCTTCATG AAGTTAATAC TTGTGAATTT ACAGGATCAA 
AGCTCTACAT TGAAGCGACT TGCTTCTCTT AAATGTTTAC GAAAACTTTG TGGTCGTTCA 
GGTTATGTAA TTCAGCCATA TTTAGATTAC CCTCCTTTGT TGGGCGCGCT AATTGGTATT 
TTGCAATCAG AACAACCAAC ACCGATAAGA AGAGAAGTAC TGAGAACATT AGGTGTGTTA 
GGTGCTTTAG ATCCATATAC GTATTTAACT ACTGAGGAAG TTTCAGATGA TTTACAAAGT 
AGTCATAATA ATGCCCATGG TGTACCTCAA ATTAGTGCCG CGCAGTACCC GTCATTGGAA 
AACTACGCTA TGGTTGCTGT TGTCACTTTG ATAGGTATTT TAAAAGACAG TTCGCTCTCT 
ATGCATCACT CTTCTGTTGT TCAAGCAGTG ATGCATATTT GCTCTCAAAT GGGATCTAAA 
TCTACTGTAT TTTTACCTCA AGTGGTACCA ACGTTTCTAC AAGTAATGCA ATCCTTATCG 
GCTTCCTCGG CTGAGTTTTA TTTTCAGCAG TTAACGACTT TAACTTCTAT TATTGGACCA 
AACATTAGGG ATTATGTATC TGATATTTTT AATCTTTCAA AAGTCTTTTG GGAATCTACC 
ACATCTCTAT TGCTAGTTAT TCTTGAACTC ATCGATGCCA TAGCAATTGC ACTGCAGGAT 
GAATTTAAAT TTTACTTGCC TCAAATATTA TCTTGTATGT TGAAAGCATT TTCCCTTGAT 
AATACGTCTT CCCGTTCCGT TTCATACAAA GTTTTACAAT CATTTGTTAT ATTTGGTTCT 
AATATTGAAG AATACATGCA TCTTGTTCTA CCAGTTATAA TTAGAAGTTT TGAGCGTGAT 
ACTATACCCC TTGGATTCAG GAAGAGTGCC CTCAAATGCA TTGCCCAATT ATTTCAATCT 
GTTAATTTCT CGGATCATGC CTCTCGAATC ATTCATCCAT TGGTTCGCAT GCTAGGGAAA 
TCTAATGGCG ATTTGAGAGC AGTTATTATG GATACTCTTT GTGCTATAGT ATCGCAACTT 
GGGTATGATT ATTCGATTTT TATACCGATG GTGAATAAAG TTTTGGTTAG TCACAAGATA 
AGTCATCCGG CTTACGAACT TTTGGTTTCA CGACTTTTGA AAGGTGAGCC TCTACCCAAA 
GATGTCGTAG TAAAGGAGTT TAAGCCGAGG CCCTCAACAA AGCCTTTTTC TACACAAGAT 
GAAGTCTTGA CAAAGCTTCC TGTTGATCAA GCATCCTTGA AAGCAGCATG GGAATCATCA 
CAAAAATTGA CAAGGGATGA TTGGCAGGAC TGGATAAGAA GAATAAGTAT TGAACTTCTG 
AAGGAATCCC CTTCTTCGGC ACTTAGGTCT TGTTCGACCT TAGCTGGTAT ATACCATCCA 
TTGGCTCGCG ATTTATTTAA TGTCAGTTTT TTGTCTTGTT GGGATGAGTT AACTGAAAGC 
AATAAGAAAA ATCTCGTTAA ATCAATAGAG CTCGCTATGA ATGCCCCTAA CATTTCAGTA 
GAAATATTAC AGACTCTGTT AAATCTTGCT GAGTATATGG AGCGTGAAGA TCATACATTA 
CCAATTCCTA TCAAAGTAAT TAGTGCTCAT GCATCTAAGT GTAATGTTTA TGCCAAGGCT 
CTGCATTATA CAGAATTACA ATTCGTTCAA GAGACGAAAG AGGAAGTTAG CATTAGCACT 
ATCGAGTCTC TCATTACCAT AAACAATCAT CTGCAACAAT CCGATGCCGC TGTAGGTATG 
CTTCAATATA CCAAAGAACA CAAGCAGTTT AGTTTGAAAG AAACTTGGTA TGAAAAATTA 
CATAGATGGG ATGATGCATT AGCTGCATAT GAGCATCGAG AACGTGAAGG TGACTCGTCT 
TTCGAGATAA ATATCGGAAA ATTACGCTGT TATTATGCTT TGGGTGACTG GGATCACCTT 
TCCGAATTGG CACAAAAAGC TTGGGTAACG TCTGAACAAG AACATCGTGA AGCTATTGCT 
CCACTTGCAG CTGCCGCTGC TTGGGGTTTG GGTCAATGGA ATTTAATTAG TGAATATGTT 
AGTGCCATGG ACCGTGACCC ACAAGATAAA GAGTTTTTTT CCGCTATTAG TGCTGTACAT 
CTTGGTCAAT ACAATAAGGC GTATGGCCAT ATCGAGAGAC ATCGTGATAT ATTAGTAAAC 
GACCTTTCTT CCATAATCGG TGAAAGCTAT AATCGTGCAT ATGGTATTAT GGTCAAATCC 
CAAATGCTTT CGGAACTTGA AGAAATTATT GACTATAAAA AGAATATGCA ATATGAAAAC 
AATTTGGACT CTCTTAAAAA AACGTGGCGG AAAAGGCTTG AAGGATGTCA GAAAAATGTG 
GATGTTTGGC ATAATACGCT TCGGTTTAGG GCTCTGGTAT TGTCTCCACA AGATTCTCCT 
GAAATGTGGA TAAAATTAGC TGATCTTTGC CGTCGTTCTG ACCGGTTGAA GCTTTCAAAT 
CAGTGTCTCA CATATTTGAT GGGTCGCGAT CCTTCAAATG CTTACCCGCT TGACTCACTT 
AAGCTTCTCA ATCCTCATGT TGTATATACG TATTTAAAAT ATTTGTGGGC TACAGATCAA 
AAAAATATTG CGGTATCGGA GCTCGAAGAG TTCACCTCAT ATCTATCGTC AAAACATGGG 
TATAAAATGG GTGATTCTTC AAAGTTGGTT GATATTTTAG CCTCATCATC TGTAAGTTCG 
GAAGAGCGTT CGTTTTTAGC AAGGTGTTTT CATAAACTTG GCAAATGGAA AAAGTCTCTT 
CAAGATAGTG TTAATCAAGA AAGCGTAAGG GATATTTTGA ATTGTTATTT TTATGCTACT 
TTGTTCGATA AATCGTGGTA TAAAGCTTGG CACTCTTGGG CTCTCGCAAA TTTCGAAGTC 
GTTGGTTATT ATGAACAGAC TGAACATGGG GTTACCCAAG ATATGTATGA ACAATATATT 
GTACCTGCTA TTAAAGGATT TTTTCACTCT TCTGTTTTAA ATCAAAAAAA CTCACTTCAG 
GATATATTGC GGTTACTTAA TTTATGGTTT AAATTCGGTG AGCACAGCGA CGTAGCAGCC 
GCAATTGTTG AAGGATTTTC CAATGTTCCA ATGGATACAT GGTTAGAAGT TATACCACAA 
CTAATTGCAC GAATACACAC TTCAAGTTCT TCAGTTAGAG CATCTGTTCA TCAGTTACTT 
TCCGATATTG GTAGGGTGCA TCCTCAGGCA TTGGTGTATT CACTTACTGT GTCCTCCAAA 
TCTACAAATC CGCAGCAGAA GCATTCAGCA AAATCAATTA TGGATAGCAT GCTTTCGCAT 
AGTGATACGT TGGTTCGTCA AGCTCTACTT GTTAGTCAAG AGCTAATTAG AGTTGCTATA 
CTTTGGCATG AATTATGGTA TGAAGGATTA GAGGAAGCTT CTCAAGCTTA TTTTTCAGAT 
CACGATATAT CTTTAATGAT TGATATTGTG AAACCCCTGC ATGAAACATT GGAGAAGGGT 
CCTTCCACCT TGTCTGAGAT ATCATTTGCT CAGACATTTG GGTATGATTT ACGTAAAGCT 
AGAAGTTACT GGCAGAAATT TTTGCAAGAT GGTGACCCTA CGGAATTAAA TCAGTCTTGG 
GATTTGTATT ATCAAGTCTT TAGACGTATT CAAAAGCAAT TACCACGGAT TAAGCATTTG 
GAGCTACAAT ATGTTTCCCC TAAGTTATTG GATGCTTGTG ATTTGGAACT AGCTGTGCCT 
GGAACCTATG GTCATAACAA ACCGGTCATT CGGATCTCGC ATTTCCATCA CACTTTCGAA 
GTTATTTCTT CTAAGCAAAG GCCAAGAAGA TTGACAATTC ATGGTAGTGA TGGCAAAGAT 
TATCAGTATG TCCTTAAGGG TCATGAAGAT TTGAGGCAAG ATGAACGAGT TATGCAGCTT 
TTTGGTTTAT GTAATACGTT ACTGACTACG GATTCTGAGA CATTCAAACG ACGTCTGAAT 
ATAGAGCGTT ATACAGTTAT TCCGCTATCT CCCAATTCTG GTTTGCTGGG GTGGGTTCCG 
CATAGTGATA CATTACATTT CCTGATCAAA GAGTTTCGCT CAAAGCGTAA CATATTGTTA 
AATTTAGAGC ATCGCATGAT GCTTCAAATG GCACCCGACT GCGACAGTTT AACATTATTG 
CAAAAACTAG AAGTATTCGA ATATGTTATG GCTAATACTG ATGGTTATGA TTTGTATCAT 
GTGTTGTGGC TTAAAAGTCG AAGTTCAGAA GCATGGCTTG ATAGGAGAAC CAGCTATACT 
CAAAGCTTGG CAGTCATGTC AATGGTTGGA TATATACTTG GACTTGGTGA CCGACATCCT 
TCCAATCTAA TGATGGATCG GTATTCTGGT AAGATCATTC ATATTGACTT TGGTGATTGT 
TTTGAGGTTG CCATGCATAG AGAAAAATTT CCTGAGAAAA TACCGTTTCG ACTTACTCGA 
ATGCTTATTA ATGCTATGGA GGTTAGCGGT ATCCAAGGTA CTTACAAAAT TACTTGCGAG 
CTTGTTATGC GTGTTTTAAG ATCAAATACG GAGTCTTTAA TGGCAGTTCT TGAAGCATTT 
GTCTATGACC CTTTGATTAA TTGGAGACTT ATGACTAAAA GCTCTTTCGG GGCTTCTACG 
ACACTCCGGC CTACCTCAAG TAGTGTAGAA GAGAAAGGAA GGTCCTACAC TCATCGTGCG 
CGTCACGCTG ATTATGCAGC TCTTAGTGAA ACAAATGGTG TAAACGCCGA AGGACTCAAC 
GAGAGATCTA TTCAAGTCTT GAAGCGTGTC TCAAATAAGC TAACTGGTAA AGATTTCGAT 
TTGAAAGAGC AATTGCCAGT CAAAGCGCAA GTTGAAAAGC TTATCCAACA AGCTACAGCT 
CCCGAAAATC TTTGCCGTTG TTATGTAGGA TGGTGTAGTT TTTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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tor1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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