SPBC2F12.10 cDNA ORF clone, Schizosaccharomyces pombe 972h-

The following SPBC2F12.10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPBC2F12.10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSj01660 NM_001021603.2
Latest version!
Schizosaccharomyces pombe 972h- mitochondrial ribosomal protein subunit L35 (predicted) (SPBC2F12.10), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OSc99999 NM_001356232.1
Latest version!
Schizosaccharomyces pombe putative mitochondrial ribosomal protein subunit L35 (SPBC2F12.10), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSj01660
    Clone ID Related Accession (Same CDS sequence) NM_001021603.2
    Accession Version NM_001021603.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2013-04-30
    Organism Schizosaccharomyces pombe 972h-
    Product mitochondrial ribosomal protein subunit L35 (predicted)
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021603.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAACGCG TATGGCCTAG AATTCCTACG ATTTCGAATG TCTGTAGGGC TCGCAGTATT 
    TACAGTGCTG CTCAGGAAAA TGCATATCGC TCTTCTGTAA AGTTAATACA GGAATACTCG
    GAGAAAGTTC ACAAAAAGCT TCAGGCTAAA TTATTAGAAA ACCCAAGTGA AACTTCCCCA
    GAAATAGAAC GGTTACAGGT TTTATCACAA ATTAATTTAC CGGAAGTACG CTCTAAATTT
    CATAAAAAGG AGATCGACTA CACGAATCCG GTGTTCCTTT ACATGCTTAA GCAACAGTGG
    GAAGATTATC AAAAATTGCT TCTTTTACAA AGATTGGAGC AGATGAAAGT AATAAAGGAC
    TCTGGGATTG GCTCATTTTC GCCATCTGTC GACGTACAGT TAGGATTTAA CCCCGAAAAT
    AATGATTCTA TCACTCCTGG CACTATCTTG CCAAGTACGG TTACTGTAAA GACCCCGTGG
    CTATCAGTTT TACCCTTTAA TTGTAAAAAG AATCATTATT CAGTTATAAC ACTAGATTTG
    GATGTCCCGA ATTACGAAAC AAACCGGTTT GAGACTCATT GCAACTGGCT TTTAACGAAT
    ATCCCCATTG AAGCATCCAA ACGTGTGCCC ATTGATACTT CTAAAGCTTT CTTTCAATAT
    CGGCCTCCTA TTGTTCACCG TGGAGAAGAT AAACACCGTA TCTTAACCCT TGTTTTGCGG
    CAAAAGTCAT CTAGTATTAG TATTCCTTCT AATGCACTTG TCCGCGAACG ATTTGACCTC
    TCTGAGTTTT GCTCAATCTA TGATCTTGAA CCTGTTGGCG CTCATCTTTG GAGAAGTGGC
    TGGGATTCGG ACGCAGTTGC ATTATTATCC AAGCACCCGT CTGTACACGA ATATCGAGAT
    ATCCGTGTAG AACGTATCCC TGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595706.2
    CDS512..1438
    Translation

    Target ORF information:

    RefSeq Version NM_001021603.2
    Organism Schizosaccharomyces pombe 972h-
    Definition Schizosaccharomyces pombe 972h- mitochondrial ribosomal protein subunit L35 (predicted) (SPBC2F12.10), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001021603.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAACGCG TATGGCCTAG AATTCCTACG ATTTCGAATG TCTGTAGGGC TCGCAGTATT 
    TACAGTGCTG CTCAGGAAAA TGCATATCGC TCTTCTGTAA AGTTAATACA GGAATACTCG
    GAGAAAGTTC ACAAAAAGCT TCAGGCTAAA TTATTAGAAA ACCCAAGTGA AACTTCCCCA
    GAAATAGAAC GGTTACAGGT TTTATCACAA ATTAATTTAC CGGAAGTACG CTCTAAATTT
    CATAAAAAGG AGATCGACTA CACGAATCCG GTGTTCCTTT ACATGCTTAA GCAACAGTGG
    GAAGATTATC AAAAATTGCT TCTTTTACAA AGATTGGAGC AGATGAAAGT AATAAAGGAC
    TCTGGGATTG GCTCATTTTC GCCATCTGTC GACGTACAGT TAGGATTTAA CCCCGAAAAT
    AATGATTCTA TCACTCCTGG CACTATCTTG CCAAGTACGG TTACTGTAAA GACCCCGTGG
    CTATCAGTTT TACCCTTTAA TTGTAAAAAG AATCATTATT CAGTTATAAC ACTAGATTTG
    GATGTCCCGA ATTACGAAAC AAACCGGTTT GAGACTCATT GCAACTGGCT TTTAACGAAT
    ATCCCCATTG AAGCATCCAA ACGTGTGCCC ATTGATACTT CTAAAGCTTT CTTTCAATAT
    CGGCCTCCTA TTGTTCACCG TGGAGAAGAT AAACACCGTA TCTTAACCCT TGTTTTGCGG
    CAAAAGTCAT CTAGTATTAG TATTCCTTCT AATGCACTTG TCCGCGAACG ATTTGACCTC
    TCTGAGTTTT GCTCAATCTA TGATCTTGAA CCTGTTGGCG CTCATCTTTG GAGAAGTGGC
    TGGGATTCGG ACGCAGTTGC ATTATTATCC AAGCACCCGT CTGTACACGA ATATCGAGAT
    ATCCGTGTAG AACGTATCCC TGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSc99999
    Clone ID Related Accession (Same CDS sequence) NM_001356232.1
    Accession Version NM_001356232.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative mitochondrial ribosomal protein subunit L35
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAACGCG TATGGCCTAG AATTCCTACG ATTTCGAATG TCTGTAGGGC TCGCAGTATT 
    TACAGTGCTG CTCAGGAAAA TGCATATCGC TCTTCTGTAA AGTTAATACA GGAATACTCG
    GAGAAAGTTC ACAAAAAGCT TCAGGCTAAA TTATTAGAAA ACCCAAGTGA AACTTCCCCA
    GAAATAGAAC GGTTACAGGT TTTATCACAA ATTAATTTAC CGGAAGTACG CTCTAAATTT
    CATAAAAAGG AGATCGACTA CACGAATCCG GTGTTCCTTT ACATGCTTAA GCAACAGTGG
    GAAGATTATC AAAAATTGCT TCTTTTACAA AGATTGGAGC AGATGAAAGT AATAAAGGAC
    TCTGGGATTG GCTCATTTTC GCCATCTGTC GACGTACAGT TAGGATTTAA CCCCGAAAAT
    AATGATTCTA TCACTCCTGG CACTATCTTG CCAAGTACGG TTACTGTAAA GACCCCGTGG
    CTATCAGTTT TACCCTTTAA TTGTAAAAAG AATCATTATT CAGTTATAAC ACTAGATTTG
    GATGTCCCGA ATTACGAAAC AAACCGGTTT GAGACTCATT GCAACTGGCT TTTAACGAAT
    ATCCCCATTG AAGCATCCAA ACGTGTGCCC ATTGATACTT CTAAAGCTTT CTTTCAATAT
    CGGCCTCCTA TTGTTCACCG TGGAGAAGAT AAACACCGTA TCTTAACCCT TGTTTTGCGG
    CAAAAGTCAT CTAGTATTAG TATTCCTTCT AATGCACTTG TCCGCGAACG ATTTGACCTC
    TCTGAGTTTT GCTCAATCTA TGATCTTGAA CCTGTTGGCG CTCATCTTTG GAGAAGTGGC
    TGGGATTCGG ACGCAGTTGC ATTATTATCC AAGCACCCGT CTGTACACGA ATATCGAGAT
    ATCCGTGTAG AACGTATCCC TGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342831.1
    CDS470..1396
    Translation

    Target ORF information:

    RefSeq Version NM_001356232.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative mitochondrial ribosomal protein subunit L35 (SPBC2F12.10), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001356232.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAACGCG TATGGCCTAG AATTCCTACG ATTTCGAATG TCTGTAGGGC TCGCAGTATT 
    TACAGTGCTG CTCAGGAAAA TGCATATCGC TCTTCTGTAA AGTTAATACA GGAATACTCG
    GAGAAAGTTC ACAAAAAGCT TCAGGCTAAA TTATTAGAAA ACCCAAGTGA AACTTCCCCA
    GAAATAGAAC GGTTACAGGT TTTATCACAA ATTAATTTAC CGGAAGTACG CTCTAAATTT
    CATAAAAAGG AGATCGACTA CACGAATCCG GTGTTCCTTT ACATGCTTAA GCAACAGTGG
    GAAGATTATC AAAAATTGCT TCTTTTACAA AGATTGGAGC AGATGAAAGT AATAAAGGAC
    TCTGGGATTG GCTCATTTTC GCCATCTGTC GACGTACAGT TAGGATTTAA CCCCGAAAAT
    AATGATTCTA TCACTCCTGG CACTATCTTG CCAAGTACGG TTACTGTAAA GACCCCGTGG
    CTATCAGTTT TACCCTTTAA TTGTAAAAAG AATCATTATT CAGTTATAAC ACTAGATTTG
    GATGTCCCGA ATTACGAAAC AAACCGGTTT GAGACTCATT GCAACTGGCT TTTAACGAAT
    ATCCCCATTG AAGCATCCAA ACGTGTGCCC ATTGATACTT CTAAAGCTTT CTTTCAATAT
    CGGCCTCCTA TTGTTCACCG TGGAGAAGAT AAACACCGTA TCTTAACCCT TGTTTTGCGG
    CAAAAGTCAT CTAGTATTAG TATTCCTTCT AATGCACTTG TCCGCGAACG ATTTGACCTC
    TCTGAGTTTT GCTCAATCTA TGATCTTGAA CCTGTTGGCG CTCATCTTTG GAGAAGTGGC
    TGGGATTCGG ACGCAGTTGC ATTATTATCC AAGCACCCGT CTGTACACGA ATATCGAGAT
    ATCCGTGTAG AACGTATCCC TGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L