mad1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following mad1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mad1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100251 NM_001021425.2
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Schizosaccharomyces pombe mitotic spindle checkpoint protein Mad1 (mad1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100251
    Clone ID Related Accession (Same CDS sequence) NM_001021425.2
    Accession Version NM_001021425.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product mitotic spindle checkpoint protein Mad1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021425.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAGCTCCA AGCTGACAGT GTATCAAGCG ACTACATCCA TGGCGGATTC TCCTAGGGAT 
    CCGTTCCAAT CGCGTTCACA GTTGCCTAGA TTTCTAGCAA CAAGCGTTAA GAAACCAAAT
    TTGAAGAAGC CTTCTGTTAA TTCTGCTAAT GAAACAAAAA ATCCCAAACT AGCTTCTTTA
    GAATTTCAAC TAGAAAACCT AAAAAATGAT TTAAAGCGGA AGGAACTGGA ATTTGAGCGT
    GAACAAATTG AGTTGCAAAG AAAACTTGCA GAAGAACATG AACAGAAGAA TTCGTTACAG
    CTGCGTCTAA CTCTAGTTGA AAAGCAACTG GAGGAGCAGT CTACTTCTTA TCAAAAAGAA
    ATAGAAGAAG TAAGAAATGA AAAGGAAGCC ACTCAGGTGA AGATCCATGA ACTACTTGAT
    GCAAAATGGA AAGAGATTGC AGAGTTGAAA ACCCAGATAG AAAAGAATGA TCAAGCTTTA
    AGTGAAAAGA ATCATGAAGT AATGGTCTCA AATCAAGCTT TGCAAATGAA GGATACCAAT
    CTTACAAACC TTGAAAAACT CTTTGCGGAT TCCCGCGAAC AGCTTGAAAC GAAGTGTAAG
    GAACTTGCGG CTGCCGAGCA GCAATTACAA GAGCTTTCAG TTCATAATCA GCAATTGGAA
    GAGTCCATCA AGCAGGTTTC TAGTTCTATT GAACTGGAAA AAATAAATGC TGAGCAACGT
    CTTCAAATTA GCGAGTTGGA AAAATTAAAA GCTGCGCAGG AAGAACGAAT TGAAAAGCTA
    TCATCCAATA ATAGGAATGT TGAAATTCTC AAGGAAGAGA AAAACGATTT GGAGTCCAAA
    CTTTATCGTT TTGAAGAATA TAGGGATAAG GTTGCTACCC TTGAACTCGA AAATGAAAAA
    ATTCAAACTG AATTAAATTC TTGGAAGAGT TTAATTACTA ACGAGTTACC AACCCCTGAA
    GCTGTTTCAA ACAAACTCGT GTTTCTACAG AATACCAATG CTAATCTCGG AGAGCGCGTT
    TCTAGTTTAG AGAGTCAATT GTCTAATAAG CCTGCAAACC AGCCGCTTGG TGCAAATGAA
    AAAGACGCCG CTCATATTAC CGAACTTGAA ACAAAGCTTA AAGAACTTCA TGAGCAGAAC
    CGACGTCTAC AAAGACAAAA ATCTTTGGCA ACCCAAGAAA TTGACCTTTT AAGGGAAAAC
    CTTAAAAGCT ATGATGATGA GGAGGCGATT TTGAGTGAAA AAAATACTGA TATGAAGAAG
    TTGGAAAGAA TTGAAGGTCT GGTCAAGCTG GTTGATGAAT ATAAGCTAAA GCTTGAAAGT
    ATGCCTGTAT CTCTTGATGT AGATGAGACT TCAGATGAGG TCTCATTACA AAAGAGACGC
    AGGAAAAACG AACATAAAGA TGCTGGTTAC GTAACGGAGC TATATCGTAA GAATCAGCAT
    TTGCTTTTTC AAGTTAAAGA GAAAACCAAC ATTGAAGCCT TTCTAAGGGA GCAGATTATT
    ACATTGGAAT CCTCCATAGC AACTTTACGT CAAGAACTGG CACAAGTTAC TGAAATAAAC
    TCATGTCGAG TATTACAGCA TCGATCCAAT CCTACTTTGA AATATGAGAG GATTAAAGCT
    GCGCAATTGG AGATGCTGAA TGCTGAGAAC TCAGCTTTGA AAGCTCTTTT GGAAGATAAA
    AAGGTTGATT GTCTACCTAT ACAATCATTT AAAATAGCTG AAAGAAAGGC CTTGGATTTA
    AAGAAAGAAG TAGCCGAGAG AGAAAAGAGA ATTCAACGGT TAAAGGAGAT TTTTTCAGTA
    AAGTCTTTGG AATTTCGCGA AGCTGTTTTT TCGCTTTTTG GATATAAGCT CGATTTTATG
    CCTAATGGGA GTGTTCGTGT TACATCCACA TATTCTCGTG AAGATAATAC CGCTTTTATA
    TTTGATGGCG AATCCAGCAC CATGAAATTG GTTGGTAATC CATCAGGCCC CGAATTTGAG
    CGCCTTATAC GATTTTGGTG TGATGAACGC AAAACAATAC CAGGCATGTT AGCGGCTCTA
    ACGTTAGAGC TTCTTGACAA AAATGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595516.1
    CDS1..2070
    Translation

    Target ORF information:

    RefSeq Version NM_001021425.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe mitotic spindle checkpoint protein Mad1 (mad1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001021425.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAGCTCCA AGCTGACAGT GTATCAAGCG ACTACATCCA TGGCGGATTC TCCTAGGGAT 
    CCGTTCCAAT CGCGTTCACA GTTGCCTAGA TTTCTAGCAA CAAGCGTTAA GAAACCAAAT
    TTGAAGAAGC CTTCTGTTAA TTCTGCTAAT GAAACAAAAA ATCCCAAACT AGCTTCTTTA
    GAATTTCAAC TAGAAAACCT AAAAAATGAT TTAAAGCGGA AGGAACTGGA ATTTGAGCGT
    GAACAAATTG AGTTGCAAAG AAAACTTGCA GAAGAACATG AACAGAAGAA TTCGTTACAG
    CTGCGTCTAA CTCTAGTTGA AAAGCAACTG GAGGAGCAGT CTACTTCTTA TCAAAAAGAA
    ATAGAAGAAG TAAGAAATGA AAAGGAAGCC ACTCAGGTGA AGATCCATGA ACTACTTGAT
    GCAAAATGGA AAGAGATTGC AGAGTTGAAA ACCCAGATAG AAAAGAATGA TCAAGCTTTA
    AGTGAAAAGA ATCATGAAGT AATGGTCTCA AATCAAGCTT TGCAAATGAA GGATACCAAT
    CTTACAAACC TTGAAAAACT CTTTGCGGAT TCCCGCGAAC AGCTTGAAAC GAAGTGTAAG
    GAACTTGCGG CTGCCGAGCA GCAATTACAA GAGCTTTCAG TTCATAATCA GCAATTGGAA
    GAGTCCATCA AGCAGGTTTC TAGTTCTATT GAACTGGAAA AAATAAATGC TGAGCAACGT
    CTTCAAATTA GCGAGTTGGA AAAATTAAAA GCTGCGCAGG AAGAACGAAT TGAAAAGCTA
    TCATCCAATA ATAGGAATGT TGAAATTCTC AAGGAAGAGA AAAACGATTT GGAGTCCAAA
    CTTTATCGTT TTGAAGAATA TAGGGATAAG GTTGCTACCC TTGAACTCGA AAATGAAAAA
    ATTCAAACTG AATTAAATTC TTGGAAGAGT TTAATTACTA ACGAGTTACC AACCCCTGAA
    GCTGTTTCAA ACAAACTCGT GTTTCTACAG AATACCAATG CTAATCTCGG AGAGCGCGTT
    TCTAGTTTAG AGAGTCAATT GTCTAATAAG CCTGCAAACC AGCCGCTTGG TGCAAATGAA
    AAAGACGCCG CTCATATTAC CGAACTTGAA ACAAAGCTTA AAGAACTTCA TGAGCAGAAC
    CGACGTCTAC AAAGACAAAA ATCTTTGGCA ACCCAAGAAA TTGACCTTTT AAGGGAAAAC
    CTTAAAAGCT ATGATGATGA GGAGGCGATT TTGAGTGAAA AAAATACTGA TATGAAGAAG
    TTGGAAAGAA TTGAAGGTCT GGTCAAGCTG GTTGATGAAT ATAAGCTAAA GCTTGAAAGT
    ATGCCTGTAT CTCTTGATGT AGATGAGACT TCAGATGAGG TCTCATTACA AAAGAGACGC
    AGGAAAAACG AACATAAAGA TGCTGGTTAC GTAACGGAGC TATATCGTAA GAATCAGCAT
    TTGCTTTTTC AAGTTAAAGA GAAAACCAAC ATTGAAGCCT TTCTAAGGGA GCAGATTATT
    ACATTGGAAT CCTCCATAGC AACTTTACGT CAAGAACTGG CACAAGTTAC TGAAATAAAC
    TCATGTCGAG TATTACAGCA TCGATCCAAT CCTACTTTGA AATATGAGAG GATTAAAGCT
    GCGCAATTGG AGATGCTGAA TGCTGAGAAC TCAGCTTTGA AAGCTCTTTT GGAAGATAAA
    AAGGTTGATT GTCTACCTAT ACAATCATTT AAAATAGCTG AAAGAAAGGC CTTGGATTTA
    AAGAAAGAAG TAGCCGAGAG AGAAAAGAGA ATTCAACGGT TAAAGGAGAT TTTTTCAGTA
    AAGTCTTTGG AATTTCGCGA AGCTGTTTTT TCGCTTTTTG GATATAAGCT CGATTTTATG
    CCTAATGGGA GTGTTCGTGT TACATCCACA TATTCTCGTG AAGATAATAC CGCTTTTATA
    TTTGATGGCG AATCCAGCAC CATGAAATTG GTTGGTAATC CATCAGGCCC CGAATTTGAG
    CGCCTTATAC GATTTTGGTG TGATGAACGC AAAACAATAC CAGGCATGTT AGCGGCTCTA
    ACGTTAGAGC TTCTTGACAA AAATGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L