spb70 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following spb70 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spb70 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99241 NM_001022758.2
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Schizosaccharomyces pombe DNA polymerase alpha B-subunit (spb70), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc99241
    Clone ID Related Accession (Same CDS sequence) NM_001022758.2
    Accession Version NM_001022758.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product DNA polymerase alpha B-subunit
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001022758.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAATTTC CTATCGATGA TGAGGAATTG CTAGATGAGC GAAATAAACT TCTCCAAATT 
    TGCAATATGG ACGAGCAAAC AATGTTCTAC AAGTGGGAGT CCTGGTGTTT GCAACGAGGA
    AATACACCAA AACTCGACCT TGACACATTC AAGGCCTTTG CTAAAGACAT GAAATTTCAA
    ATGGAGAGGC AGGTAAAAGC CACACTTAAA CAGAATCCTG AAAGAAAATC GATAAAGCAA
    ATCCCGGGAA TGAATATTGA TAGCATACTA GGTCTACCAG TGAAGACCAC CGCATCAGGC
    GACTCGTTGA TGCAAGAGTC TAAACCATCT ACCGAAACTT TCGAATTGAA TTCCAGTGAC
    GCTGGAAGGG TTTTGGAAGT TTTAAATAAA GAATTAAAAA TTGTAACTTC AAAACCTTCA
    GCTCCATCCA AGCTGGTTAT CGTAGCGAAT TTTGATCTCA AGGCATTTAA TTACAGGATC
    ATGTATCAAA AGCTTTATGA TTCTTCTGAA GTTCTGGATG ATCGCATTGA GCTTTTTAGT
    GCATTAACTT GCAGAAAATA CAACATAAGT GATGAAGATT TGGCAAATCC TAGCGAACTT
    ACTCAAGAAC CCGTTGTAGT TGTAGGACGA ATTGTTGTCG AAAGTACCAA TTTAGGTGGA
    AGACTCAACC AAAATAGCAT TTTACTTGAA AGTTCAAGAC GTCTAGGCGC TGGTGTTCGA
    GTTCGTTTGA AGGTGGATGA TCTACCAAGT TACTCAATAT TTCCTGGTCA AATTGTTTCA
    GTTAAAGGAT CCAATCCTAG CGGTAACATG TTTATCGCCA AGGAAATCCT TCCTATACCT
    CCTTTGCCGT TCCCAAGTTC ATCTAAACAG GAGCACGCTA CCTTTGTTGC TAATACAAAT
    AATCAGCCAA TTTCTATATA TATTGCATCT GGACCTTGGT CTCTCCGTGA TGATCTCTCT
    TTTTCCCCTT TAAAATCAAT GATTTCATAT GTAAATAAAA ATCCTGTTGA CTTGGTTATT
    CTATGTGGTC CATTCTTGGA TATTAATCAT ATACTAATAC GTACCGGAAA CATTACAGGA
    ACGTCTGCGA CTTCTTTGGA AGAGTTGTTT AAAGAGCGTG TCACACCCAT ATTAAGCCAG
    CTAACTTGTC CTTGTATTTT AATTCCTCAC ATCAATGATG CTGCTTCAGA TCATCCTGCA
    TGGCCACAGG ATGCATTTAA TCGGGTGGCC TTAGGTCTTC CATCTAACTT CAAATGCTTC
    CCGAATCCTT GCATGTTTTC CATTAATGAT GTCGTCTTTG GTGTGTCAAC CAACGATATA
    CTCCTTCACA CATCTAGAGA AGAGTTATTT CGTCTACCTT CTCATGGTAA CTTATTTGCA
    CGTTTGGTTT CTCACGTACT TCACCAAAGG CATTTTTATC CCTTATTCCC TGGAGGTTCT
    CTTGAAAAGT GCAACCCTTC AAACTTAGAT ATCGCACATC TTAAACTTGG TGAATTCCTA
    AATACTATGC CGGACATACT AATTTTACCA AGTGATTTAC GTTATTTCGT AAAGAACGTT
    GAAAACGTTG TATCATTGAA TCCTGGTAAA GCGACCAAAG GGATTAATTT GGGCACTTTT
    GCAAAATTAA CTATCGCGCC ATTAGAGCTC GGGGATAATG GTTCTTCTAA TCATTATTCA
    CATCGTGTAT GGCTTCGAAC CAAGGCAGAA ATTTTAAAAC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_587765.1
    CDS424..2148
    Translation

    Target ORF information:

    RefSeq Version NM_001022758.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe DNA polymerase alpha B-subunit (spb70), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001022758.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAATTTC CTATCGATGA TGAGGAATTG CTAGATGAGC GAAATAAACT TCTCCAAATT 
    TGCAATATGG ACGAGCAAAC AATGTTCTAC AAGTGGGAGT CCTGGTGTTT GCAACGAGGA
    AATACACCAA AACTCGACCT TGACACATTC AAGGCCTTTG CTAAAGACAT GAAATTTCAA
    ATGGAGAGGC AGGTAAAAGC CACACTTAAA CAGAATCCTG AAAGAAAATC GATAAAGCAA
    ATCCCGGGAA TGAATATTGA TAGCATACTA GGTCTACCAG TGAAGACCAC CGCATCAGGC
    GACTCGTTGA TGCAAGAGTC TAAACCATCT ACCGAAACTT TCGAATTGAA TTCCAGTGAC
    GCTGGAAGGG TTTTGGAAGT TTTAAATAAA GAATTAAAAA TTGTAACTTC AAAACCTTCA
    GCTCCATCCA AGCTGGTTAT CGTAGCGAAT TTTGATCTCA AGGCATTTAA TTACAGGATC
    ATGTATCAAA AGCTTTATGA TTCTTCTGAA GTTCTGGATG ATCGCATTGA GCTTTTTAGT
    GCATTAACTT GCAGAAAATA CAACATAAGT GATGAAGATT TGGCAAATCC TAGCGAACTT
    ACTCAAGAAC CCGTTGTAGT TGTAGGACGA ATTGTTGTCG AAAGTACCAA TTTAGGTGGA
    AGACTCAACC AAAATAGCAT TTTACTTGAA AGTTCAAGAC GTCTAGGCGC TGGTGTTCGA
    GTTCGTTTGA AGGTGGATGA TCTACCAAGT TACTCAATAT TTCCTGGTCA AATTGTTTCA
    GTTAAAGGAT CCAATCCTAG CGGTAACATG TTTATCGCCA AGGAAATCCT TCCTATACCT
    CCTTTGCCGT TCCCAAGTTC ATCTAAACAG GAGCACGCTA CCTTTGTTGC TAATACAAAT
    AATCAGCCAA TTTCTATATA TATTGCATCT GGACCTTGGT CTCTCCGTGA TGATCTCTCT
    TTTTCCCCTT TAAAATCAAT GATTTCATAT GTAAATAAAA ATCCTGTTGA CTTGGTTATT
    CTATGTGGTC CATTCTTGGA TATTAATCAT ATACTAATAC GTACCGGAAA CATTACAGGA
    ACGTCTGCGA CTTCTTTGGA AGAGTTGTTT AAAGAGCGTG TCACACCCAT ATTAAGCCAG
    CTAACTTGTC CTTGTATTTT AATTCCTCAC ATCAATGATG CTGCTTCAGA TCATCCTGCA
    TGGCCACAGG ATGCATTTAA TCGGGTGGCC TTAGGTCTTC CATCTAACTT CAAATGCTTC
    CCGAATCCTT GCATGTTTTC CATTAATGAT GTCGTCTTTG GTGTGTCAAC CAACGATATA
    CTCCTTCACA CATCTAGAGA AGAGTTATTT CGTCTACCTT CTCATGGTAA CTTATTTGCA
    CGTTTGGTTT CTCACGTACT TCACCAAAGG CATTTTTATC CCTTATTCCC TGGAGGTTCT
    CTTGAAAAGT GCAACCCTTC AAACTTAGAT ATCGCACATC TTAAACTTGG TGAATTCCTA
    AATACTATGC CGGACATACT AATTTTACCA AGTGATTTAC GTTATTTCGT AAAGAACGTT
    GAAAACGTTG TATCATTGAA TCCTGGTAAA GCGACCAAAG GGATTAATTT GGGCACTTTT
    GCAAAATTAA CTATCGCGCC ATTAGAGCTC GGGGATAATG GTTCTTCTAA TCATTATTCA
    CATCGTGTAT GGCTTCGAAC CAAGGCAGAA ATTTTAAAAC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L