VHA-A cDNA ORF clone, Arabidopsis thaliana(thale cress)

The following VHA-A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VHA-A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAb31273 NM_001036222.3
Latest version!
Arabidopsis thaliana vacuolar ATP synthase subunit A (VHA-A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OAb31273 NM_106539.6
Latest version!
Arabidopsis thaliana vacuolar ATP synthase subunit A (VHA-A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAb31273
    Clone ID Related Accession (Same CDS sequence) NM_106539.6 , NM_001036222.3
    Accession Version NM_106539.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product vacuolar ATP synthase subunit A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_106539.5.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCGGCGT TTTACGGAGG AAAGCTTACG ACCTTCGAGG ACGATGAGAA GGAGAGCGAG 
    TATGGTTACG TTCGTAAGGT TTCAGGTCCT GTTGTTGTTG CCGATGGTAT GGCCGGTGCT
    GCTATGTATG AGTTAGTGCG TGTTGGTCAT GATAATTTGA TTGGTGAAAT CATCCGTCTT
    GAAGGAGATT CTGCCACCAT CCAAGTTTAC GAGGAAACAG CTGGATTGAC AGTTAATGAT
    CCCGTTCTTC GAACACACAA GCCACTTTCT GTGGAGCTCG GGCCAGGAAT ATTGGGAAAT
    ATCTTTGATG GAATTCAGAG GCCTTTGAAG ACTATTGCAA GAATATCCGG TGATGTGTAC
    ATTCCTCGGG GTGTGTCTGT TCCAGCTCTT GACAAAGATT GTCTTTGGGA GTTCCAGCCC
    AATAAATTTG TCGAGGGAGA CACAATAACT GGTGGTGACT TGTATGCTAC TGTCTTTGAG
    AACACTTTGA TGAATCACCT CGTTGCCCTT CCTCCGGATG CCATGGGGAA GATCACTTAC
    ATTGCTCCAG CTGGTCAATA TTCGCTTAAG GATACCGTGA TAGAGCTTGA ATTCCAGGGG
    ATCAAGAAAT CTTACACCAT GCTTCAGAGC TGGCCTGTAC GTACGCCTAG GCCAGTTGCA
    TCAAAGCTTG CTGCCGATAC TCCTCTACTT ACGGGGCAGC GTGTTCTTGA TGCCCTTTTC
    CCTTCTGTTC TTGGTGGAAC CTGTGCCATT CCTGGTGCTT TTGGCTGTGG GAAAACTGTT
    ATCAGTCAGG CACTTTCCAA GTACTCCAAC TCTGATGCTG TTGTGTATGT TGGTTGTGGA
    GAGAGAGGAA ATGAAATGGC TGAGGTTCTT ATGGACTTCC CACAATTGAC AATGACGTTG
    CCTGATGGCC GTGAGGAATC TGTCATGAAA CGTACCACAC TTGTTGCTAA CACCTCTAAC
    ATGCCTGTGG CTGCTCGTGA AGCCTCAATT TACACAGGAA TCACAATCGC TGAATATTTT
    AGAGATATGG GCTACAATGT TAGTATGATG GCAGACTCAA CTTCCCGTTG GGCAGAAGCA
    TTAAGAGAAA TTTCAGGACG GCTGGCTGAA ATGCCTGCTG ACAGTGGATA TCCAGCCTAT
    CTAGCAGCAC GTTTAGCATC TTTCTATGAA CGTGCTGGTA AAGTAAAATG TCTTGGTGGA
    CCAGAACGTA ACGGAAGTGT TACAATTGTT GGTGCAGTTT CGCCTCCTGG AGGAGACTTT
    TCAGATCCTG TGACTTCAGC AACCCTTAGT ATTGTGCAGG TCTTCTGGGG TTTGGACAAA
    AAGCTTGCCC AGAGAAAACA TTTTCCCTCT GTTAATTGGT TGATTTCTTA CTCAAAGTAT
    TCAACGGCAC TGGAATCTTT CTATGAGAAG TTCGATCCAG ATTTCATCAA CATCAGGACA
    AAGGCCAGAG AGGTGTTGCA GAGGGAAGAC GATCTTAATG AAATTGTCCA GCTTGTAGGA
    AAAGATGCGC TAGCAGAAGG GGACAAAATC ACATTGGAAA CAGCTAAGCT ATTGAGGGAA
    GATTACCTTG CTCAAAACGC GTTTACACCA TATGACAAAT TCTGTCCTTT CTACAAGTCC
    GTGTGGATGA TGCGTAACAT TATCCATTTC TACAACCTAG CCAACCAGGC GGTTGAGAGA
    GCAGCTGGAA TGGACGGTCA AAAGATTACC TACACTCTTA TCAAGCATCG CTTAGGAGAT
    CTTTTCTACC GTTTAGTGTC TCAGAAGTTC GAAGACCCAG CAGAAGGGGA GGATACACTG
    GTGGAAAAAT TCAAGAAATT GTACGACGAT CTCAATGCTG GATTCCGTGC TTTGGAAGAT
    GAAACTCGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_178011.1
    CDS225..2096
    Translation

    Target ORF information:

    RefSeq Version NM_106539.6
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana vacuolar ATP synthase subunit A (VHA-A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_106539.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCGGCGT TTTACGGAGG AAAGCTTACG ACCTTCGAGG ACGATGAGAA GGAGAGCGAG 
    TATGGTTACG TTCGTAAGGT TTCAGGTCCT GTTGTTGTTG CCGATGGTAT GGCCGGTGCT
    GCTATGTATG AGTTAGTGCG TGTTGGTCAT GATAATTTGA TTGGTGAAAT CATCCGTCTT
    GAAGGAGATT CTGCCACCAT CCAAGTTTAC GAGGAAACAG CTGGATTGAC AGTTAATGAT
    CCCGTTCTTC GAACACACAA GCCACTTTCT GTGGAGCTCG GGCCAGGAAT ATTGGGAAAT
    ATCTTTGATG GAATTCAGAG GCCTTTGAAG ACTATTGCAA GAATATCCGG TGATGTGTAC
    ATTCCTCGGG GTGTGTCTGT TCCAGCTCTT GACAAAGATT GTCTTTGGGA GTTCCAGCCC
    AATAAATTTG TCGAGGGAGA CACAATAACT GGTGGTGACT TGTATGCTAC TGTCTTTGAG
    AACACTTTGA TGAATCACCT CGTTGCCCTT CCTCCGGATG CCATGGGGAA GATCACTTAC
    ATTGCTCCAG CTGGTCAATA TTCGCTTAAG GATACCGTGA TAGAGCTTGA ATTCCAGGGG
    ATCAAGAAAT CTTACACCAT GCTTCAGAGC TGGCCTGTAC GTACGCCTAG GCCAGTTGCA
    TCAAAGCTTG CTGCCGATAC TCCTCTACTT ACGGGGCAGC GTGTTCTTGA TGCCCTTTTC
    CCTTCTGTTC TTGGTGGAAC CTGTGCCATT CCTGGTGCTT TTGGCTGTGG GAAAACTGTT
    ATCAGTCAGG CACTTTCCAA GTACTCCAAC TCTGATGCTG TTGTGTATGT TGGTTGTGGA
    GAGAGAGGAA ATGAAATGGC TGAGGTTCTT ATGGACTTCC CACAATTGAC AATGACGTTG
    CCTGATGGCC GTGAGGAATC TGTCATGAAA CGTACCACAC TTGTTGCTAA CACCTCTAAC
    ATGCCTGTGG CTGCTCGTGA AGCCTCAATT TACACAGGAA TCACAATCGC TGAATATTTT
    AGAGATATGG GCTACAATGT TAGTATGATG GCAGACTCAA CTTCCCGTTG GGCAGAAGCA
    TTAAGAGAAA TTTCAGGACG GCTGGCTGAA ATGCCTGCTG ACAGTGGATA TCCAGCCTAT
    CTAGCAGCAC GTTTAGCATC TTTCTATGAA CGTGCTGGTA AAGTAAAATG TCTTGGTGGA
    CCAGAACGTA ACGGAAGTGT TACAATTGTT GGTGCAGTTT CGCCTCCTGG AGGAGACTTT
    TCAGATCCTG TGACTTCAGC AACCCTTAGT ATTGTGCAGG TCTTCTGGGG TTTGGACAAA
    AAGCTTGCCC AGAGAAAACA TTTTCCCTCT GTTAATTGGT TGATTTCTTA CTCAAAGTAT
    TCAACGGCAC TGGAATCTTT CTATGAGAAG TTCGATCCAG ATTTCATCAA CATCAGGACA
    AAGGCCAGAG AGGTGTTGCA GAGGGAAGAC GATCTTAATG AAATTGTCCA GCTTGTAGGA
    AAAGATGCGC TAGCAGAAGG GGACAAAATC ACATTGGAAA CAGCTAAGCT ATTGAGGGAA
    GATTACCTTG CTCAAAACGC GTTTACACCA TATGACAAAT TCTGTCCTTT CTACAAGTCC
    GTGTGGATGA TGCGTAACAT TATCCATTTC TACAACCTAG CCAACCAGGC GGTTGAGAGA
    GCAGCTGGAA TGGACGGTCA AAAGATTACC TACACTCTTA TCAAGCATCG CTTAGGAGAT
    CTTTTCTACC GTTTAGTGTC TCAGAAGTTC GAAGACCCAG CAGAAGGGGA GGATACACTG
    GTGGAAAAAT TCAAGAAATT GTACGACGAT CTCAATGCTG GATTCCGTGC TTTGGAAGAT
    GAAACTCGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb31273
    Clone ID Related Accession (Same CDS sequence) NM_106539.6 , NM_001036222.3
    Accession Version NM_001036222.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product vacuolar ATP synthase subunit A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_001036222.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCGGCGT TTTACGGAGG AAAGCTTACG ACCTTCGAGG ACGATGAGAA GGAGAGCGAG 
    TATGGTTACG TTCGTAAGGT TTCAGGTCCT GTTGTTGTTG CCGATGGTAT GGCCGGTGCT
    GCTATGTATG AGTTAGTGCG TGTTGGTCAT GATAATTTGA TTGGTGAAAT CATCCGTCTT
    GAAGGAGATT CTGCCACCAT CCAAGTTTAC GAGGAAACAG CTGGATTGAC AGTTAATGAT
    CCCGTTCTTC GAACACACAA GCCACTTTCT GTGGAGCTCG GGCCAGGAAT ATTGGGAAAT
    ATCTTTGATG GAATTCAGAG GCCTTTGAAG ACTATTGCAA GAATATCCGG TGATGTGTAC
    ATTCCTCGGG GTGTGTCTGT TCCAGCTCTT GACAAAGATT GTCTTTGGGA GTTCCAGCCC
    AATAAATTTG TCGAGGGAGA CACAATAACT GGTGGTGACT TGTATGCTAC TGTCTTTGAG
    AACACTTTGA TGAATCACCT CGTTGCCCTT CCTCCGGATG CCATGGGGAA GATCACTTAC
    ATTGCTCCAG CTGGTCAATA TTCGCTTAAG GATACCGTGA TAGAGCTTGA ATTCCAGGGG
    ATCAAGAAAT CTTACACCAT GCTTCAGAGC TGGCCTGTAC GTACGCCTAG GCCAGTTGCA
    TCAAAGCTTG CTGCCGATAC TCCTCTACTT ACGGGGCAGC GTGTTCTTGA TGCCCTTTTC
    CCTTCTGTTC TTGGTGGAAC CTGTGCCATT CCTGGTGCTT TTGGCTGTGG GAAAACTGTT
    ATCAGTCAGG CACTTTCCAA GTACTCCAAC TCTGATGCTG TTGTGTATGT TGGTTGTGGA
    GAGAGAGGAA ATGAAATGGC TGAGGTTCTT ATGGACTTCC CACAATTGAC AATGACGTTG
    CCTGATGGCC GTGAGGAATC TGTCATGAAA CGTACCACAC TTGTTGCTAA CACCTCTAAC
    ATGCCTGTGG CTGCTCGTGA AGCCTCAATT TACACAGGAA TCACAATCGC TGAATATTTT
    AGAGATATGG GCTACAATGT TAGTATGATG GCAGACTCAA CTTCCCGTTG GGCAGAAGCA
    TTAAGAGAAA TTTCAGGACG GCTGGCTGAA ATGCCTGCTG ACAGTGGATA TCCAGCCTAT
    CTAGCAGCAC GTTTAGCATC TTTCTATGAA CGTGCTGGTA AAGTAAAATG TCTTGGTGGA
    CCAGAACGTA ACGGAAGTGT TACAATTGTT GGTGCAGTTT CGCCTCCTGG AGGAGACTTT
    TCAGATCCTG TGACTTCAGC AACCCTTAGT ATTGTGCAGG TCTTCTGGGG TTTGGACAAA
    AAGCTTGCCC AGAGAAAACA TTTTCCCTCT GTTAATTGGT TGATTTCTTA CTCAAAGTAT
    TCAACGGCAC TGGAATCTTT CTATGAGAAG TTCGATCCAG ATTTCATCAA CATCAGGACA
    AAGGCCAGAG AGGTGTTGCA GAGGGAAGAC GATCTTAATG AAATTGTCCA GCTTGTAGGA
    AAAGATGCGC TAGCAGAAGG GGACAAAATC ACATTGGAAA CAGCTAAGCT ATTGAGGGAA
    GATTACCTTG CTCAAAACGC GTTTACACCA TATGACAAAT TCTGTCCTTT CTACAAGTCC
    GTGTGGATGA TGCGTAACAT TATCCATTTC TACAACCTAG CCAACCAGGC GGTTGAGAGA
    GCAGCTGGAA TGGACGGTCA AAAGATTACC TACACTCTTA TCAAGCATCG CTTAGGAGAT
    CTTTTCTACC GTTTAGTGTC TCAGAAGTTC GAAGACCCAG CAGAAGGGGA GGATACACTG
    GTGGAAAAAT TCAAGAAATT GTACGACGAT CTCAATGCTG GATTCCGTGC TTTGGAAGAT
    GAAACTCGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001031299.1
    CDS266..2137
    Translation

    Target ORF information:

    RefSeq Version NM_001036222.3
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana vacuolar ATP synthase subunit A (VHA-A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001036222.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCGGCGT TTTACGGAGG AAAGCTTACG ACCTTCGAGG ACGATGAGAA GGAGAGCGAG 
    TATGGTTACG TTCGTAAGGT TTCAGGTCCT GTTGTTGTTG CCGATGGTAT GGCCGGTGCT
    GCTATGTATG AGTTAGTGCG TGTTGGTCAT GATAATTTGA TTGGTGAAAT CATCCGTCTT
    GAAGGAGATT CTGCCACCAT CCAAGTTTAC GAGGAAACAG CTGGATTGAC AGTTAATGAT
    CCCGTTCTTC GAACACACAA GCCACTTTCT GTGGAGCTCG GGCCAGGAAT ATTGGGAAAT
    ATCTTTGATG GAATTCAGAG GCCTTTGAAG ACTATTGCAA GAATATCCGG TGATGTGTAC
    ATTCCTCGGG GTGTGTCTGT TCCAGCTCTT GACAAAGATT GTCTTTGGGA GTTCCAGCCC
    AATAAATTTG TCGAGGGAGA CACAATAACT GGTGGTGACT TGTATGCTAC TGTCTTTGAG
    AACACTTTGA TGAATCACCT CGTTGCCCTT CCTCCGGATG CCATGGGGAA GATCACTTAC
    ATTGCTCCAG CTGGTCAATA TTCGCTTAAG GATACCGTGA TAGAGCTTGA ATTCCAGGGG
    ATCAAGAAAT CTTACACCAT GCTTCAGAGC TGGCCTGTAC GTACGCCTAG GCCAGTTGCA
    TCAAAGCTTG CTGCCGATAC TCCTCTACTT ACGGGGCAGC GTGTTCTTGA TGCCCTTTTC
    CCTTCTGTTC TTGGTGGAAC CTGTGCCATT CCTGGTGCTT TTGGCTGTGG GAAAACTGTT
    ATCAGTCAGG CACTTTCCAA GTACTCCAAC TCTGATGCTG TTGTGTATGT TGGTTGTGGA
    GAGAGAGGAA ATGAAATGGC TGAGGTTCTT ATGGACTTCC CACAATTGAC AATGACGTTG
    CCTGATGGCC GTGAGGAATC TGTCATGAAA CGTACCACAC TTGTTGCTAA CACCTCTAAC
    ATGCCTGTGG CTGCTCGTGA AGCCTCAATT TACACAGGAA TCACAATCGC TGAATATTTT
    AGAGATATGG GCTACAATGT TAGTATGATG GCAGACTCAA CTTCCCGTTG GGCAGAAGCA
    TTAAGAGAAA TTTCAGGACG GCTGGCTGAA ATGCCTGCTG ACAGTGGATA TCCAGCCTAT
    CTAGCAGCAC GTTTAGCATC TTTCTATGAA CGTGCTGGTA AAGTAAAATG TCTTGGTGGA
    CCAGAACGTA ACGGAAGTGT TACAATTGTT GGTGCAGTTT CGCCTCCTGG AGGAGACTTT
    TCAGATCCTG TGACTTCAGC AACCCTTAGT ATTGTGCAGG TCTTCTGGGG TTTGGACAAA
    AAGCTTGCCC AGAGAAAACA TTTTCCCTCT GTTAATTGGT TGATTTCTTA CTCAAAGTAT
    TCAACGGCAC TGGAATCTTT CTATGAGAAG TTCGATCCAG ATTTCATCAA CATCAGGACA
    AAGGCCAGAG AGGTGTTGCA GAGGGAAGAC GATCTTAATG AAATTGTCCA GCTTGTAGGA
    AAAGATGCGC TAGCAGAAGG GGACAAAATC ACATTGGAAA CAGCTAAGCT ATTGAGGGAA
    GATTACCTTG CTCAAAACGC GTTTACACCA TATGACAAAT TCTGTCCTTT CTACAAGTCC
    GTGTGGATGA TGCGTAACAT TATCCATTTC TACAACCTAG CCAACCAGGC GGTTGAGAGA
    GCAGCTGGAA TGGACGGTCA AAAGATTACC TACACTCTTA TCAAGCATCG CTTAGGAGAT
    CTTTTCTACC GTTTAGTGTC TCAGAAGTTC GAAGACCCAG CAGAAGGGGA GGATACACTG
    GTGGAAAAAT TCAAGAAATT GTACGACGAT CTCAATGCTG GATTCCGTGC TTTGGAAGAT
    GAAACTCGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
    Nature408(6814)816-20(2000 Dec)
    Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW