AT1G73850 cDNA ORF clone, Arabidopsis thaliana(thale cress)

The following AT1G73850 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT1G73850 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAb04314 NM_106045.4
Latest version!
Arabidopsis thaliana DNA ligase (DUF1666) (AT1G73850), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OAb04314 NM_001334608.1
Latest version!
Arabidopsis thaliana DNA ligase (DUF1666) (AT1G73850), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAb04314
    Clone ID Related Accession (Same CDS sequence) NM_106045.4 , NM_001334608.1
    Accession Version NM_106045.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product DNA ligase (DUF1666)
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_106045.3.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCAATGTT ATACAAATGA AGAAGCACTA GCAAGTCTCT TCATCAATGT CTCAACATCT 
    TTCTTTCTTC TCCTCATCCT CCTCTACTCC GCCGTCTCCC TCCTAATCAA GCTTCTCCAC
    TTCATCGGCG GCTACCCTCT CCTCCAAAGG AATGAAGATG AATATGATTC CGCGGCATGG
    TATTCCGAAG AAGAGGAGGA AGAGGAAGAA GAAGGGGAAG AACTCAAGAT GTCGTGTAAC
    TCAAGTTACC ATGTGATATC TAGAGATCCA AATCCGGATT TGATAGCTGA TATAGCAGAC
    GACGGAGAAT CTTTAGTGTT TTACAATAAT ATTTCTCAAG GAGGAGTAAA TAATCAACAC
    ACGAAGGAGT TCAATAATGT CTATCAAACT CATGAACACG AAGAAGAGGA AGAAGATGAT
    CAAGATTCTA ACACTTCTTC TACAGAGGAG CACTTTTCTT CTGCCAATGT TTCTCCTTAT
    CGCAGTGAAT CATCAATAGA AGAGGAAGAT GATGGCGTTG AAGATCTTCC TGACGATCGT
    TATGATGATG ATGACGAAGA TGAAGAGGTA GGAGGAGTGT CACGATACGA CGTCGTTGAG
    GATTTGGTTC GTAAGCAACC AAATACTACT AGTACTCGTG GTCCCTCTCG TTTCCAAAGT
    GGTTTGGTGT TCAATGACAA AAGTTACAAT GCAAGAGAGT TTGTGTCCAA TGGAGGAATC
    AAGAATGATG TCGACGAGAT ACAGCCATCA TCGGGCTTTG ACATGAGAGA GATAAAAGCA
    GAGGAATTAG AGGAAGAAGA AGAAGAAGAG AGAGGACAAA TATTCGGAGA GTCATGCACG
    AATGGGTCAA CGTCGAAAAG CTCATCGGAA TGGAGAAACT CTGTCAAAAC TGACGACCCT
    TTCTCAACGT CCTCTCGCCG GAGTTGCCCT AAATGGGAAT CTTACACCGT CTTCCAAAAG
    TACGACGAAG AAATGACTTT TCTCACTAGA ATCTCTGCTC AAAAGCTTCA CGAAGCAGAA
    TCTTTGAAGT CAATTATGGT GGAGCCAAGA TCTATCTCGG AGAGGATCGT TCACAAGTTA
    TCATCAAACG GTCACAAGAA AAAACAAAAA CAATATCCAG GCAGCAATGG GTCGAGACCA
    AACCCTTACG TGGAACTAGA ATCCGCTTAC GTGGCACAGA TATGTCTAAC ATGGGAAGCT
    CTAAGTTGGA ACTACAAAAA CTTCGAGAGA AAAAGATCAA CCACGCAACG AAGCTTCAAC
    GACGTAGGAT GTCCCGCCGG AATCGCCGAC CAATTCCGTA CGTTTCACAT TCTTTTACAA
    AGATACGTAG AAAATGAACC TTATGAACAT GGAAGAAGAC CAGAGATTTA TGCTCGCATG
    CGAACTCTTG CTCCTAAATT GCTTCTTGTT CCTGAATACC AAGATTATGA GGAAGAGGAG
    GAGAAGGAAG ACGAGAACGA AGAGGGTTTC AGGTCGAGGA TATCGTCAGC TTCGTTCTTG
    ATGATAATGG AGGAATGCAT AAGAACATTC ATGAATTTTC TCCAAGCGGA TAAAGAAAAG
    CCTTGTCAGA AGATCATCAA AGCCTTTTTT GGGAGAAGTA AACGAGGCCC CGTTGATCCA
    ACCCTTGTAC ATCTCATGAA GAAAGTCAAC ACAAAGAAAA AGACGAAGCT GAAAGAGATG
    CGTCGAGGAG GGAAATACAT GAGGAAGAAG AAGATGAGTA TAGAGGAGGA AATGGAGATA
    TTGATGGGAT TAATAGATTT GAAAGTGGTT TCAAGAGTGT TAAGGATGAA TGAAATGAAC
    GAAGAGAATT TGCATTGGTG TGAAGAGAAG ATGAGCAAAG TTAAAATTAT TCAAGGTGGA
    AAGGTGCTTC AAAGAGATTC CACTCCACTT TTCTTTCCTC CACATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_177526.3
    CDS198..2105
    Translation

    Target ORF information:

    RefSeq Version NM_106045.4
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana DNA ligase (DUF1666) (AT1G73850), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_106045.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCAATGTT ATACAAATGA AGAAGCACTA GCAAGTCTCT TCATCAATGT CTCAACATCT 
    TTCTTTCTTC TCCTCATCCT CCTCTACTCC GCCGTCTCCC TCCTAATCAA GCTTCTCCAC
    TTCATCGGCG GCTACCCTCT CCTCCAAAGG AATGAAGATG AATATGATTC CGCGGCATGG
    TATTCCGAAG AAGAGGAGGA AGAGGAAGAA GAAGGGGAAG AACTCAAGAT GTCGTGTAAC
    TCAAGTTACC ATGTGATATC TAGAGATCCA AATCCGGATT TGATAGCTGA TATAGCAGAC
    GACGGAGAAT CTTTAGTGTT TTACAATAAT ATTTCTCAAG GAGGAGTAAA TAATCAACAC
    ACGAAGGAGT TCAATAATGT CTATCAAACT CATGAACACG AAGAAGAGGA AGAAGATGAT
    CAAGATTCTA ACACTTCTTC TACAGAGGAG CACTTTTCTT CTGCCAATGT TTCTCCTTAT
    CGCAGTGAAT CATCAATAGA AGAGGAAGAT GATGGCGTTG AAGATCTTCC TGACGATCGT
    TATGATGATG ATGACGAAGA TGAAGAGGTA GGAGGAGTGT CACGATACGA CGTCGTTGAG
    GATTTGGTTC GTAAGCAACC AAATACTACT AGTACTCGTG GTCCCTCTCG TTTCCAAAGT
    GGTTTGGTGT TCAATGACAA AAGTTACAAT GCAAGAGAGT TTGTGTCCAA TGGAGGAATC
    AAGAATGATG TCGACGAGAT ACAGCCATCA TCGGGCTTTG ACATGAGAGA GATAAAAGCA
    GAGGAATTAG AGGAAGAAGA AGAAGAAGAG AGAGGACAAA TATTCGGAGA GTCATGCACG
    AATGGGTCAA CGTCGAAAAG CTCATCGGAA TGGAGAAACT CTGTCAAAAC TGACGACCCT
    TTCTCAACGT CCTCTCGCCG GAGTTGCCCT AAATGGGAAT CTTACACCGT CTTCCAAAAG
    TACGACGAAG AAATGACTTT TCTCACTAGA ATCTCTGCTC AAAAGCTTCA CGAAGCAGAA
    TCTTTGAAGT CAATTATGGT GGAGCCAAGA TCTATCTCGG AGAGGATCGT TCACAAGTTA
    TCATCAAACG GTCACAAGAA AAAACAAAAA CAATATCCAG GCAGCAATGG GTCGAGACCA
    AACCCTTACG TGGAACTAGA ATCCGCTTAC GTGGCACAGA TATGTCTAAC ATGGGAAGCT
    CTAAGTTGGA ACTACAAAAA CTTCGAGAGA AAAAGATCAA CCACGCAACG AAGCTTCAAC
    GACGTAGGAT GTCCCGCCGG AATCGCCGAC CAATTCCGTA CGTTTCACAT TCTTTTACAA
    AGATACGTAG AAAATGAACC TTATGAACAT GGAAGAAGAC CAGAGATTTA TGCTCGCATG
    CGAACTCTTG CTCCTAAATT GCTTCTTGTT CCTGAATACC AAGATTATGA GGAAGAGGAG
    GAGAAGGAAG ACGAGAACGA AGAGGGTTTC AGGTCGAGGA TATCGTCAGC TTCGTTCTTG
    ATGATAATGG AGGAATGCAT AAGAACATTC ATGAATTTTC TCCAAGCGGA TAAAGAAAAG
    CCTTGTCAGA AGATCATCAA AGCCTTTTTT GGGAGAAGTA AACGAGGCCC CGTTGATCCA
    ACCCTTGTAC ATCTCATGAA GAAAGTCAAC ACAAAGAAAA AGACGAAGCT GAAAGAGATG
    CGTCGAGGAG GGAAATACAT GAGGAAGAAG AAGATGAGTA TAGAGGAGGA AATGGAGATA
    TTGATGGGAT TAATAGATTT GAAAGTGGTT TCAAGAGTGT TAAGGATGAA TGAAATGAAC
    GAAGAGAATT TGCATTGGTG TGAAGAGAAG ATGAGCAAAG TTAAAATTAT TCAAGGTGGA
    AAGGTGCTTC AAAGAGATTC CACTCCACTT TTCTTTCCTC CACATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb04314
    Clone ID Related Accession (Same CDS sequence) NM_106045.4 , NM_001334608.1
    Accession Version NM_001334608.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product DNA ligase (DUF1666)
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCAATGTT ATACAAATGA AGAAGCACTA GCAAGTCTCT TCATCAATGT CTCAACATCT 
    TTCTTTCTTC TCCTCATCCT CCTCTACTCC GCCGTCTCCC TCCTAATCAA GCTTCTCCAC
    TTCATCGGCG GCTACCCTCT CCTCCAAAGG AATGAAGATG AATATGATTC CGCGGCATGG
    TATTCCGAAG AAGAGGAGGA AGAGGAAGAA GAAGGGGAAG AACTCAAGAT GTCGTGTAAC
    TCAAGTTACC ATGTGATATC TAGAGATCCA AATCCGGATT TGATAGCTGA TATAGCAGAC
    GACGGAGAAT CTTTAGTGTT TTACAATAAT ATTTCTCAAG GAGGAGTAAA TAATCAACAC
    ACGAAGGAGT TCAATAATGT CTATCAAACT CATGAACACG AAGAAGAGGA AGAAGATGAT
    CAAGATTCTA ACACTTCTTC TACAGAGGAG CACTTTTCTT CTGCCAATGT TTCTCCTTAT
    CGCAGTGAAT CATCAATAGA AGAGGAAGAT GATGGCGTTG AAGATCTTCC TGACGATCGT
    TATGATGATG ATGACGAAGA TGAAGAGGTA GGAGGAGTGT CACGATACGA CGTCGTTGAG
    GATTTGGTTC GTAAGCAACC AAATACTACT AGTACTCGTG GTCCCTCTCG TTTCCAAAGT
    GGTTTGGTGT TCAATGACAA AAGTTACAAT GCAAGAGAGT TTGTGTCCAA TGGAGGAATC
    AAGAATGATG TCGACGAGAT ACAGCCATCA TCGGGCTTTG ACATGAGAGA GATAAAAGCA
    GAGGAATTAG AGGAAGAAGA AGAAGAAGAG AGAGGACAAA TATTCGGAGA GTCATGCACG
    AATGGGTCAA CGTCGAAAAG CTCATCGGAA TGGAGAAACT CTGTCAAAAC TGACGACCCT
    TTCTCAACGT CCTCTCGCCG GAGTTGCCCT AAATGGGAAT CTTACACCGT CTTCCAAAAG
    TACGACGAAG AAATGACTTT TCTCACTAGA ATCTCTGCTC AAAAGCTTCA CGAAGCAGAA
    TCTTTGAAGT CAATTATGGT GGAGCCAAGA TCTATCTCGG AGAGGATCGT TCACAAGTTA
    TCATCAAACG GTCACAAGAA AAAACAAAAA CAATATCCAG GCAGCAATGG GTCGAGACCA
    AACCCTTACG TGGAACTAGA ATCCGCTTAC GTGGCACAGA TATGTCTAAC ATGGGAAGCT
    CTAAGTTGGA ACTACAAAAA CTTCGAGAGA AAAAGATCAA CCACGCAACG AAGCTTCAAC
    GACGTAGGAT GTCCCGCCGG AATCGCCGAC CAATTCCGTA CGTTTCACAT TCTTTTACAA
    AGATACGTAG AAAATGAACC TTATGAACAT GGAAGAAGAC CAGAGATTTA TGCTCGCATG
    CGAACTCTTG CTCCTAAATT GCTTCTTGTT CCTGAATACC AAGATTATGA GGAAGAGGAG
    GAGAAGGAAG ACGAGAACGA AGAGGGTTTC AGGTCGAGGA TATCGTCAGC TTCGTTCTTG
    ATGATAATGG AGGAATGCAT AAGAACATTC ATGAATTTTC TCCAAGCGGA TAAAGAAAAG
    CCTTGTCAGA AGATCATCAA AGCCTTTTTT GGGAGAAGTA AACGAGGCCC CGTTGATCCA
    ACCCTTGTAC ATCTCATGAA GAAAGTCAAC ACAAAGAAAA AGACGAAGCT GAAAGAGATG
    CGTCGAGGAG GGAAATACAT GAGGAAGAAG AAGATGAGTA TAGAGGAGGA AATGGAGATA
    TTGATGGGAT TAATAGATTT GAAAGTGGTT TCAAGAGTGT TAAGGATGAA TGAAATGAAC
    GAAGAGAATT TGCATTGGTG TGAAGAGAAG ATGAGCAAAG TTAAAATTAT TCAAGGTGGA
    AAGGTGCTTC AAAGAGATTC CACTCCACTT TTCTTTCCTC CACATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001319378.1
    CDS107..2014
    Translation

    Target ORF information:

    RefSeq Version NM_001334608.1
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana DNA ligase (DUF1666) (AT1G73850), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001334608.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCAATGTT ATACAAATGA AGAAGCACTA GCAAGTCTCT TCATCAATGT CTCAACATCT 
    TTCTTTCTTC TCCTCATCCT CCTCTACTCC GCCGTCTCCC TCCTAATCAA GCTTCTCCAC
    TTCATCGGCG GCTACCCTCT CCTCCAAAGG AATGAAGATG AATATGATTC CGCGGCATGG
    TATTCCGAAG AAGAGGAGGA AGAGGAAGAA GAAGGGGAAG AACTCAAGAT GTCGTGTAAC
    TCAAGTTACC ATGTGATATC TAGAGATCCA AATCCGGATT TGATAGCTGA TATAGCAGAC
    GACGGAGAAT CTTTAGTGTT TTACAATAAT ATTTCTCAAG GAGGAGTAAA TAATCAACAC
    ACGAAGGAGT TCAATAATGT CTATCAAACT CATGAACACG AAGAAGAGGA AGAAGATGAT
    CAAGATTCTA ACACTTCTTC TACAGAGGAG CACTTTTCTT CTGCCAATGT TTCTCCTTAT
    CGCAGTGAAT CATCAATAGA AGAGGAAGAT GATGGCGTTG AAGATCTTCC TGACGATCGT
    TATGATGATG ATGACGAAGA TGAAGAGGTA GGAGGAGTGT CACGATACGA CGTCGTTGAG
    GATTTGGTTC GTAAGCAACC AAATACTACT AGTACTCGTG GTCCCTCTCG TTTCCAAAGT
    GGTTTGGTGT TCAATGACAA AAGTTACAAT GCAAGAGAGT TTGTGTCCAA TGGAGGAATC
    AAGAATGATG TCGACGAGAT ACAGCCATCA TCGGGCTTTG ACATGAGAGA GATAAAAGCA
    GAGGAATTAG AGGAAGAAGA AGAAGAAGAG AGAGGACAAA TATTCGGAGA GTCATGCACG
    AATGGGTCAA CGTCGAAAAG CTCATCGGAA TGGAGAAACT CTGTCAAAAC TGACGACCCT
    TTCTCAACGT CCTCTCGCCG GAGTTGCCCT AAATGGGAAT CTTACACCGT CTTCCAAAAG
    TACGACGAAG AAATGACTTT TCTCACTAGA ATCTCTGCTC AAAAGCTTCA CGAAGCAGAA
    TCTTTGAAGT CAATTATGGT GGAGCCAAGA TCTATCTCGG AGAGGATCGT TCACAAGTTA
    TCATCAAACG GTCACAAGAA AAAACAAAAA CAATATCCAG GCAGCAATGG GTCGAGACCA
    AACCCTTACG TGGAACTAGA ATCCGCTTAC GTGGCACAGA TATGTCTAAC ATGGGAAGCT
    CTAAGTTGGA ACTACAAAAA CTTCGAGAGA AAAAGATCAA CCACGCAACG AAGCTTCAAC
    GACGTAGGAT GTCCCGCCGG AATCGCCGAC CAATTCCGTA CGTTTCACAT TCTTTTACAA
    AGATACGTAG AAAATGAACC TTATGAACAT GGAAGAAGAC CAGAGATTTA TGCTCGCATG
    CGAACTCTTG CTCCTAAATT GCTTCTTGTT CCTGAATACC AAGATTATGA GGAAGAGGAG
    GAGAAGGAAG ACGAGAACGA AGAGGGTTTC AGGTCGAGGA TATCGTCAGC TTCGTTCTTG
    ATGATAATGG AGGAATGCAT AAGAACATTC ATGAATTTTC TCCAAGCGGA TAAAGAAAAG
    CCTTGTCAGA AGATCATCAA AGCCTTTTTT GGGAGAAGTA AACGAGGCCC CGTTGATCCA
    ACCCTTGTAC ATCTCATGAA GAAAGTCAAC ACAAAGAAAA AGACGAAGCT GAAAGAGATG
    CGTCGAGGAG GGAAATACAT GAGGAAGAAG AAGATGAGTA TAGAGGAGGA AATGGAGATA
    TTGATGGGAT TAATAGATTT GAAAGTGGTT TCAAGAGTGT TAAGGATGAA TGAAATGAAC
    GAAGAGAATT TGCATTGGTG TGAAGAGAAG ATGAGCAAAG TTAAAATTAT TCAAGGTGGA
    AAGGTGCTTC AAAGAGATTC CACTCCACTT TTCTTTCCTC CACATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
    Nature408(6814)816-20(2000 Dec)
    Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW