ORF » Species Summary » Arabidopsis thaliana » EMB25 cDNA ORF clone

EMB25 cDNA ORF clone, Arabidopsis thaliana(thale cress)

The following EMB25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMB25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAb27539 NM_105675.2
Latest version!		 Arabidopsis thaliana DEAD/DEAH box helicase (EMB25), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    EMB25 ( NM_105675.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_177164.1 Arabidopsis thaliana DEAD/DEAH box helicase (EMB25), partial mRNA.
    Price & Availability↑
    CloneID OAb27539
    Clone ID Related Accession (Same CDS sequence) NM_105675.2
    Accession Version NM_105675.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product DEAD/DEAH box helicase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On May 13, 2003 this sequence version replaced NM_105675.1. COMPLETENESS: incomplete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACACTC TTCCCGTCGT CTCTCTCACA GCTTCTTCTT CCTTCAAATT CTTCCATTTT 
    CCTTCTCTGC ACCGTTCTCT CTCTCATTCC CCAAACTTCT CCTTCACCAA ATCCCTTATC 
    CTCAATCCAA ACCACCTCTC CTTCAAATCC ACCTTAAATT CTCTTTCACC GTCTCAGTCT 
    CAGCTCTACG AGGAAGAAGA CGACGAAGAA GAAGAAGAAG AAGACGAAGA TGATGATGAC 
    GAAGCTGCTG ACGAATACGA CAATATCTCC GATGAAATTC GGAATAGCGA TGACGACGAC 
    GACGATGAAG AAACCGAATT CTCCGTGGAT TTGCCGACGG AATCAGCTCG GGAACGAGTG 
    GAGTTCCGGT GGCAGAGAGT GGAGAAGCTT CGTAGTCTTG TTAGAGATTT TGGTGTTGAG 
    ATGATTGATA TCGATGAACT CATTTCAATC TACGATTTTC GAATCGACAA GTTTCAGAGA 
    TTGGCTATTG AAGCTTTTCT TAGAGGTTCT TCTGTTGTGG TTTCTGCTCC GACTAGTAGT 
    GGCAAGACTT TGATTGCTGA AGCTGCTGCT GTTTCCACGG TGGCTAAAGG TCGCCGGTTG 
    TTCTACACTA CACCTCTTAA AGCACTCTCT AATCAGAAGT TTCGTGAGTT TAGGGAGACA 
    TTTGGGGATG ATAATGTTGG TCTACTCACA GGTGACTCTG CTATTAACAA AGATGCTCAG 
    ATTGTGATTA TGACTACAGA GATTCTCCGT AACATGTTGT ATCAAAGTGT TGGAATGGCT 
    TCTTCAGGAA CTGGACTTTT TCATGTTGAT GCCATTGTTT TGGATGAGGT TCACTATTTA 
    AGTGATATAT CTAGGGGAAC TGTGTGGGAA GAGATAGTCA TTTATTGTCC AAAGGAGGTT 
    CAACTTATAT GCCTATCAGC TACAGTTGCA AATCCGGATG AATTAGCTGG TTGGATTGGT 
    GAGATACATG GTAAAACTGA GTTGGTAACA TCGACAAGAC GCCCAGTTCC ATTAACTTGG 
    TACTTTTCAA CAAAACATTC TTTATTACCT CTGCTTGATG AGAAAGGGAT AAACGTGAAT 
    AGGAAGCTTT CTCTCAATTA TTTGCAACTG TCTGCCTCAG AAGCTAGATT CCGTGATGAT 
    GATGACGGGT ATAGGAAAAG GAGGTCAAAG AAACGAGGAG GTGATACCAG CTACAACAAC 
    CTTGTGAACG TAACTGATTA TCCTCTTTCA AAGAATGAGA TAAACAAGAT TCGTCGATCA 
    CAGGTACCTC AAATTAGCGA CACATTATGG CATCTCCAGG GGAAAAATAT GTTACCAGCG 
    ATATGGTTCA TTTTTAACCG TAGAGGATGT GATGCAGCTG TACAGTATGT TGAAAATTTT 
    CAGCTTTTAG ATGATTGCGA GAAGAGTGAG GTTGAGTTGG CCTTGAAGAA GTTTCGCGTT 
    CTTTATCCAG ATGCAGTGAG AGAGAGTGCA GAGAAAGGAC TGCTTAGGGG AATTGCTGCT 
    CATCATGCAG GTTGTCTACC TCTTTGGAAA TCTTTCATTG AGGAACTTTT TCAGCGAGGA 
    CTTGTTAAGG TTGTCTTTGC TACTGAGACA CTTGCTGCTG GAATAAACAT GCCGGCTAGA 
    ACGGCTGTTA TCTCGTCTCT TTCAAAAAAG GCTGGTAATG AACGAATAGA GTTGGGTCCT 
    AACGAATTGT ACCAGATGGC TGGGCGTGCT GGACGCAGAG GCATCGATGA AAAGGGCTAC 
    ACTGTGCTGG TTCAGACTGC TTTTGAAGGC GCTGAAGAGT GCTGCAAACT TGTCTTTGCT 
    GGAGTTAAAC CTCTCGTGTC ACAATTTACT GCTTCATATG GCATGGTACT GAATCTGGTA 
    GCTGGTTCAA AAGTTACTCG TAAATCAAGT GGAACTGAGG CCGGGAAAGT CTTACAAGCA 
    GGGAGAAGTC TGGAAGAAGC CAAGAAATTA GTTGAGAAGA GTTTTGGAAA CTATGTGAGC 
    AGCAATGTGA CGGTTGCTGC GAAGCAGGAA CTTGCTGAAA TCGACAATAA GATCGAGATT 
    CTGTCATCTG AAATAAGTGA TGAAGCAATA GATAAGAAGA GTAGGAAACT TCTTTCAGCG 
    AGGGATTACA AGGAGATCAC TGTACTTAAA GAAGAGCTAC GGGAAGAGAA ACGCAAGCGT 
    GCTGAACAAC GAAGAAGAAT GGAACTCGAA AGGTTCTTAG CTCTAAAACC GCTCTTAAAA 
    GGGATGGAAG AGGGGAACTT ACCATTTATT TGTTTGGAGT TCAAGGATTC TGAAGGGAGG 
    GAGCAGTCAG TCCCTGCTGT TTACTTGGGG CATATTGATT CATTTCAGGG TTCCAAACTT 
    CAGAAGATGA TGTCTTTGGA TGAGTCTTTT GCGTTAAACT TAATCGAGGA TGAGCTGGCG 
    GCTGATGAAC CTGGAAAGCC TAATGTTAAA CCGTCTTACT ATGTGGCCCT TGGCTCTGAT 
    AACTCATGGT ATCTCTTTAC TGAGAAGTGG GTTAGGACTG TCTACAGAAC TGGTTTCCCT 
    AACATTGCTC TAGCGCTTGG AGATGCTTTG CCTCGAGAAA TCATGAAGAA TCTCCTGGAT 
    AAAGCAGATA TGCAGTGGGA TAAACTAGCT GAATCTGAAC TTGGAAGTTT GTGGAGATTG 
    GAAGGATCTC TAGAGACATG GTCCTGGAGT TTAAACGTAC CTGTCCTAAG CAGCCTTTCT 
    GACGAAGATG AGGTGTTACA TATGTCCGAA GAGTATGATA ACGCGGCGCA AAAATACAAG 
    GAACAAAGGA GCAAAATTTC GCGTTTGAAG AAGAAGATGT CCCGGTCAGA AGGGTTCAGA 
    GAATATAAGA AAATTCTAGA GAATGCTAAC TTGACGGTTG AGAAGATGAA ACGGCTCAAG 
    GCGAGATCGA GACGCCTTAT AAACCGTTTA GAGCAGATTG AACCGTCTGG TTGGAAAGAT 
    TTCATGCGCA TAAGCAACGT GATTCACGAA AGCAGAGCAT TGGACATTAA TACACACTTG 
    ATATTTCCTC TGGGAGAAAC AGCTGCTGCA ATCCGAGGAG AAAACGAGCT TTGGCTTGCA 
    ATGGTGTTGC GGAACAAAGC CCTGGTGGAT CTCAAGCCTC CTCAACTAGC TGGTGTATGT 
    GCAAGTTTAG TTTCAGAAGG CATAAAAGTT CGACCTTGGA GAGACAACAA CTATATTTAC 
    GAGCCATCTG ATACAGTAGT AGACATGGTC AATTTCTTAG AAGACCAAAG AAGCTCTCTT 
    ATAAAGCTTC AAGAGAAGCA CGAGGTTATG ATTCCATGTT GTCTGGACGT TCAGTTCTCT 
    GGTATGGTTG AAGCTTGGGC TTCTGGATTA AGTTGGAAAG AAATGATGAT GGAATGTGCA 
    ATGGACGAAG GCGATCTTGC TCGCCTGTTA CGACGCACAA TTGATCTCTT AGCTCAGATT 
    CCTAAGTTGC CTGATATTGA CCCAGTCCTG CAACGTAGTG CAGCAGCTGC AGCAGATATC 
    ATGGACCGTC CACCAATTAG TGAGCTTGCC GGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_177164.1
    CDS49..3564
    Translation

    Target ORF information:

    RefSeq Version NM_105675.2
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana DEAD/DEAH box helicase (EMB25), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_105675.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACACTC TTCCCGTCGT CTCTCTCACA GCTTCTTCTT CCTTCAAATT CTTCCATTTT 
    CCTTCTCTGC ACCGTTCTCT CTCTCATTCC CCAAACTTCT CCTTCACCAA ATCCCTTATC 
    CTCAATCCAA ACCACCTCTC CTTCAAATCC ACCTTAAATT CTCTTTCACC GTCTCAGTCT 
    CAGCTCTACG AGGAAGAAGA CGACGAAGAA GAAGAAGAAG AAGACGAAGA TGATGATGAC 
    GAAGCTGCTG ACGAATACGA CAATATCTCC GATGAAATTC GGAATAGCGA TGACGACGAC 
    GACGATGAAG AAACCGAATT CTCCGTGGAT TTGCCGACGG AATCAGCTCG GGAACGAGTG 
    GAGTTCCGGT GGCAGAGAGT GGAGAAGCTT CGTAGTCTTG TTAGAGATTT TGGTGTTGAG 
    ATGATTGATA TCGATGAACT CATTTCAATC TACGATTTTC GAATCGACAA GTTTCAGAGA 
    TTGGCTATTG AAGCTTTTCT TAGAGGTTCT TCTGTTGTGG TTTCTGCTCC GACTAGTAGT 
    GGCAAGACTT TGATTGCTGA AGCTGCTGCT GTTTCCACGG TGGCTAAAGG TCGCCGGTTG 
    TTCTACACTA CACCTCTTAA AGCACTCTCT AATCAGAAGT TTCGTGAGTT TAGGGAGACA 
    TTTGGGGATG ATAATGTTGG TCTACTCACA GGTGACTCTG CTATTAACAA AGATGCTCAG 
    ATTGTGATTA TGACTACAGA GATTCTCCGT AACATGTTGT ATCAAAGTGT TGGAATGGCT 
    TCTTCAGGAA CTGGACTTTT TCATGTTGAT GCCATTGTTT TGGATGAGGT TCACTATTTA 
    AGTGATATAT CTAGGGGAAC TGTGTGGGAA GAGATAGTCA TTTATTGTCC AAAGGAGGTT 
    CAACTTATAT GCCTATCAGC TACAGTTGCA AATCCGGATG AATTAGCTGG TTGGATTGGT 
    GAGATACATG GTAAAACTGA GTTGGTAACA TCGACAAGAC GCCCAGTTCC ATTAACTTGG 
    TACTTTTCAA CAAAACATTC TTTATTACCT CTGCTTGATG AGAAAGGGAT AAACGTGAAT 
    AGGAAGCTTT CTCTCAATTA TTTGCAACTG TCTGCCTCAG AAGCTAGATT CCGTGATGAT 
    GATGACGGGT ATAGGAAAAG GAGGTCAAAG AAACGAGGAG GTGATACCAG CTACAACAAC 
    CTTGTGAACG TAACTGATTA TCCTCTTTCA AAGAATGAGA TAAACAAGAT TCGTCGATCA 
    CAGGTACCTC AAATTAGCGA CACATTATGG CATCTCCAGG GGAAAAATAT GTTACCAGCG 
    ATATGGTTCA TTTTTAACCG TAGAGGATGT GATGCAGCTG TACAGTATGT TGAAAATTTT 
    CAGCTTTTAG ATGATTGCGA GAAGAGTGAG GTTGAGTTGG CCTTGAAGAA GTTTCGCGTT 
    CTTTATCCAG ATGCAGTGAG AGAGAGTGCA GAGAAAGGAC TGCTTAGGGG AATTGCTGCT 
    CATCATGCAG GTTGTCTACC TCTTTGGAAA TCTTTCATTG AGGAACTTTT TCAGCGAGGA 
    CTTGTTAAGG TTGTCTTTGC TACTGAGACA CTTGCTGCTG GAATAAACAT GCCGGCTAGA 
    ACGGCTGTTA TCTCGTCTCT TTCAAAAAAG GCTGGTAATG AACGAATAGA GTTGGGTCCT 
    AACGAATTGT ACCAGATGGC TGGGCGTGCT GGACGCAGAG GCATCGATGA AAAGGGCTAC 
    ACTGTGCTGG TTCAGACTGC TTTTGAAGGC GCTGAAGAGT GCTGCAAACT TGTCTTTGCT 
    GGAGTTAAAC CTCTCGTGTC ACAATTTACT GCTTCATATG GCATGGTACT GAATCTGGTA 
    GCTGGTTCAA AAGTTACTCG TAAATCAAGT GGAACTGAGG CCGGGAAAGT CTTACAAGCA 
    GGGAGAAGTC TGGAAGAAGC CAAGAAATTA GTTGAGAAGA GTTTTGGAAA CTATGTGAGC 
    AGCAATGTGA CGGTTGCTGC GAAGCAGGAA CTTGCTGAAA TCGACAATAA GATCGAGATT 
    CTGTCATCTG AAATAAGTGA TGAAGCAATA GATAAGAAGA GTAGGAAACT TCTTTCAGCG 
    AGGGATTACA AGGAGATCAC TGTACTTAAA GAAGAGCTAC GGGAAGAGAA ACGCAAGCGT 
    GCTGAACAAC GAAGAAGAAT GGAACTCGAA AGGTTCTTAG CTCTAAAACC GCTCTTAAAA 
    GGGATGGAAG AGGGGAACTT ACCATTTATT TGTTTGGAGT TCAAGGATTC TGAAGGGAGG 
    GAGCAGTCAG TCCCTGCTGT TTACTTGGGG CATATTGATT CATTTCAGGG TTCCAAACTT 
    CAGAAGATGA TGTCTTTGGA TGAGTCTTTT GCGTTAAACT TAATCGAGGA TGAGCTGGCG 
    GCTGATGAAC CTGGAAAGCC TAATGTTAAA CCGTCTTACT ATGTGGCCCT TGGCTCTGAT 
    AACTCATGGT ATCTCTTTAC TGAGAAGTGG GTTAGGACTG TCTACAGAAC TGGTTTCCCT 
    AACATTGCTC TAGCGCTTGG AGATGCTTTG CCTCGAGAAA TCATGAAGAA TCTCCTGGAT 
    AAAGCAGATA TGCAGTGGGA TAAACTAGCT GAATCTGAAC TTGGAAGTTT GTGGAGATTG 
    GAAGGATCTC TAGAGACATG GTCCTGGAGT TTAAACGTAC CTGTCCTAAG CAGCCTTTCT 
    GACGAAGATG AGGTGTTACA TATGTCCGAA GAGTATGATA ACGCGGCGCA AAAATACAAG 
    GAACAAAGGA GCAAAATTTC GCGTTTGAAG AAGAAGATGT CCCGGTCAGA AGGGTTCAGA 
    GAATATAAGA AAATTCTAGA GAATGCTAAC TTGACGGTTG AGAAGATGAA ACGGCTCAAG 
    GCGAGATCGA GACGCCTTAT AAACCGTTTA GAGCAGATTG AACCGTCTGG TTGGAAAGAT 
    TTCATGCGCA TAAGCAACGT GATTCACGAA AGCAGAGCAT TGGACATTAA TACACACTTG 
    ATATTTCCTC TGGGAGAAAC AGCTGCTGCA ATCCGAGGAG AAAACGAGCT TTGGCTTGCA 
    ATGGTGTTGC GGAACAAAGC CCTGGTGGAT CTCAAGCCTC CTCAACTAGC TGGTGTATGT 
    GCAAGTTTAG TTTCAGAAGG CATAAAAGTT CGACCTTGGA GAGACAACAA CTATATTTAC 
    GAGCCATCTG ATACAGTAGT AGACATGGTC AATTTCTTAG AAGACCAAAG AAGCTCTCTT 
    ATAAAGCTTC AAGAGAAGCA CGAGGTTATG ATTCCATGTT GTCTGGACGT TCAGTTCTCT 
    GGTATGGTTG AAGCTTGGGC TTCTGGATTA AGTTGGAAAG AAATGATGAT GGAATGTGCA 
    ATGGACGAAG GCGATCTTGC TCGCCTGTTA CGACGCACAA TTGATCTCTT AGCTCAGATT 
    CCTAAGTTGC CTGATATTGA CCCAGTCCTG CAACGTAGTG CAGCAGCTGC AGCAGATATC 
    ATGGACCGTC CACCAATTAG TGAGCTTGCC GGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
    Nature408(6814)816-20(2000 Dec)
    Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW


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