XRN4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	XRN4
Entrez Gene ID	841891
Full Name	exoribonuclease 4
Synonyms	5'-3' EXORIBONUCLEASE,ACC INSENSITIVE 1,AIN1,ATXRN4,EIN5,ETHYLENE INSENSITIVE 5,EXORIBONUCLEASE 4,F20D21.30,F20D21_30,exoribonuclease 4
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001333652.1	NP_001321327.1	exoribonuclease 4
NM_104327.4	NP_175851.1	exoribonuclease 4

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	XRN4	NP_175851.1
Oryza sativa (rice)	Os03g0794800	NP_001051542.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that specific motifs, secondary energy, and GC content of transcripts could play a role in their degradation by exoribonuclease4 (xrn4) and varicose (vcs).
Title: 5' to 3' mRNA Decay Contributes to the Regulation of Arabidopsis Seed Germination by Dormancy.

These results demonstrate that AtXRN4-mediated mRNA degradation is linked to the suppression of heat acclimation.
Title: Loss of Arabidopsis 5'-3' Exoribonuclease AtXRN4 Function Enhances Heat Stress Tolerance of Plants Subjected to Severe Heat Stress.

XRN4 is required to set up a massive heat-induced mRNA decay.
Title: XRN4 and LARP1 are required for a heat-triggered mRNA decay pathway involved in plant acclimation and survival during thermal stress.

Loss of XRN4 function can potentially trigger the cosuppression in a sequence-dependent manner.
Title: Loss of XRN4 function can trigger cosuppression in a sequence-dependent manner.

Map based cloning identified in both lines mutations in XRN4 (Exoribonuclease 4), which was previously described as a suppressor of transgene silencing in source leaves.
Title: The second face of a known player: Arabidopsis silencing suppressor AtXRN4 acts organ-specifically.

The following XRN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the XRN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb26388	NM_104327.4 Latest version!	Arabidopsis thaliana exoribonuclease 4 (XRN4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb34443	NM_001333652.1 Latest version!	Arabidopsis thaliana exoribonuclease 4 (XRN4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

XRN4 ( NM_104327.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_175851.1 Arabidopsis thaliana exoribonuclease 4 (XRN4), mRNA.

Price & Availability↑

CloneID	OAb26388
Clone ID Related Accession (Same CDS sequence)	NM_104327.4
Accession Version	NM_104327.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	exoribonuclease 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_104327.3.

ATGGGAGTAC CGGCGTTCTA CAGATGGCTC GCTGACCGAT ACCCGAAGTC AATTAGTGAC 
GTCGTTGAAG AAGAACCTAC CGATGGAGGT CGTGGTGACC TTATTCCTGT TGATATCACC 
AGACCTAACC CTAACGGCTT CGAATTTGAT AATTTGTATC TCGATATGAA TGGTATCATT 
CATCCTTGTT TCCATCCTGA AGGAAAGCCT GCACCTGCCA CGTACGATGA TGTATTTAAA 
TCAATGTTTG AGTACATTGA CCATCTTTTT ACTTTAGTGC GTCCGAGGAA GATTCTTTAT 
TTGGCTATAG ATGGAGTTGC GCCGAGAGCA AAGATGAATC AGCAGCGTTC TCGACGTTTC 
AGAGCTGCAA AGGACGCAGC AGAGGCTGAA GCTGAGGAAG AGAGGTTGAG AAAAGATTTT 
GAGATGGAGG GCCAAATCTT GTCCGCTAAA GAGAAAGCTG AAACCTGTGA TTCAAATGTC 
ATTACTCCTG GGACCCCTTT TATGGCTATA CTGTCAGTCG CTCTTCAGTA TTACATCCAA 
TCTAGGTTAA ACCATAACCC TGGCTGGAGA TATGTTAAGG TAATTCTTTC TGATTCAAAT 
GTTCCGGGAG AAGGAGAACA CAAGATAATG TCATACATTC GTCTCCAACG TAATCTTCCT 
GGTTTTGATC CAAATACACG GCATTGTCTA TATGGTCTGG ATGCAGATTT GATAATGCTC 
TCCTTAGCTA CACATGAAGT TCACTTTTCC ATCTTAAGAG AGGTTATCAC TTACCCTGGG 
CAGCAAGAGA AATGCTTTGT GTGTGGACAA ACTGGCCACT TTGCTTCAGA TTGCCCTGGA 
AAATCAGGTA GCAATAATGC AGCAGCTGAT ATTCCAATCC ACAAGAAAAA ATATCAGTTC 
CTGAACATTT GGGTGTTGCG AGAATATCTG CAATATGAAT TGGCCATTCC AGATCCTCCT 
TTTATGATCA ACTTTGAGAG AATAATAGAC GACTTTGTAT TCCTTTGCTT TTTTGTTGGT 
AATGACTTCC TACCTCATAT GCCTACACTG GAAATTCGAG AGGGGGCTAT AAACTTGCTG 
ATGCATGTTT ACAGAAAGGA GTTTACAGCT ATGGGAGGCT ATCTAACAGA TTCTGGTGAG 
GTTTTACTAG ATAGGGTAGA GCATTTCATT CAAGCTGTCG CTGTAAATGA AGATAAAATA 
TTTCAGAAAA GAACGCGGAT AAAACAGTCA ATGGATAACA ATGAAGAAAT GAAGCAAAGA 
TCTAGAAGGG ACCCATCTGA GGTACCACCT GAACCGATAG ACGATAAGAT TAAATTAGGA 
GAACCAGGCT ACAAGGAGAG GTATTATGCT GAGAAATTTA GTACGACCAA TCCAGAGGAA 
ACTGAGCAAA TCAAACAAGA CATGGTACTG AAATATGTTG AAGGTTTGTG CTGGGTTTGC 
CGGTACTACT ACCAAGGTGT ATGCTCTTGG CAATGGTTTT ACCCATACCA TTATGCTCCA 
TTTGCTTCAG ATCTTAAGAA TCTACCTGAT TTAGAAATCA CATTTTTTAT TGGAGAGCCC 
TTTAAACCTT TTGACCAGTT AATGGGAACC CTGCCGGCTG CAAGCTCAAA TGCGCTGCCT 
GGAGAATACC GGAAGTTGAT GACTGATCCC TCATCCCCGA TACTTAAATT TTACCCTGCT 
GATTTTGAGC TTGACATGAA TGGAAAGCGC TTCGCCTGGC AGGGTATTGC AAAACTTCCT 
TTCATTGAGG AGAAATTATT GTTAGCTGCT ACAAGGAAGC TTGAAGAAAC TCTAACGGTG 
GAAGAACAAC AACGCAACAG TGTGATGCTT GATTTGTTAT ACGTACACCC CGCTCATCCA 
CTGGGCCAGC GGATTCTGCA GTACTATCAT TTCTACCAGC ACATGCCACC ACATGAATGT 
CTTCCATGGA TGATTGACCC GAATTCTAGC CAAGGAATGA ACGGTTTTCT TTGGTTTAGT 
GAAAGGAATG GTTTCCAAAC TAGAGTGGAT TCCCCTGTCA ATGGTTTGCC ATGCATTGAG 
CAGAACCGAG CCTTAAATGT TACATACCTG TGTCCTGCCA AACACTCTCA TATCTCAGAA 
CCACCCAGAG GAGCGATCAT TCCGGACAAG ATCTTGACGT CAGTAGATAT AAAGCCATTT 
CCTCCTTTAT GGCATGAAGA CAATAGCAAC CGACGCCGAC AAGCCCGAGA TAGGCCGCAA 
GTGGTTGGAG CTATAGCAGG GCCTTCTCTG GGAGAAGCAG CTCATCGTCT GATCAAAAAC 
ACCCTTAACA TGAAATCCTC TACTGGTGCG GCTTCGGGAT TAATTGACCC AAACGGATAC 
TACCGAAATG TACCGGGTAA TTATTCTTAT GGTGGAGTCA ATAGACCAAG AGCACCGGGT 
CCGTCTCCTT ACCGGAAAGC TTATGACGAT GACTCGAGCT ATTACTATGG AAAGTATAAC 
AATAGCACAC AGGGAACGTT TAACAATGGT CCAAGATATC CTTATCCATC AAATGGGTCA 
CAAGACTATA ACCGGAACTA CAACTCCAAG ATAGTAGCAG AACAGCACAA CCGTGGTGGA 
CTTGGAGCTG GAATGTCTGG TTTATCGATA GAAGATAACG GCAGAAGCAA ACAATTGTAT 
TCAAGTTATA CAGAAGCTGC TAATGCAAAT TTGAACCCGT TGCCATCACC CCCCACCCAA 
TGGATCGGTA CACAGCCAGG TGGTAATTTT GTCGGTGGAT ACTATAGGGA TGGAGTTGGG 
TACAGTGAAA CAAATGGAAA ATCGGTAAAG AAAGTAATTT ACCAGGCCAA GACACAACCA 
AGTCATCGAG GTGCAAACTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_175851.1
CDS	192..3035
Translation	MGVPAFYRWLADRYPKSISDVVEEEPTDGGRGDLIPVDITRPNPNGFEFDNLYLDMNGIIHPCFHPEGKPAPATYDDVFKSMFEYIDHLFTLVRPRKILYLAIDGVAPRAKMNQQRSRRFRAAKDAAEAEAEEERLRKDFEMEGQILSAKEKAETCDSNVITPGTPFMAILSVALQYYIQSRLNHNPGWRYVKVILSDSNVPGEGEHKIMSYIRLQRNLPGFDPNTRHCLYGLDADLIMLSLATHEVHFSILREVITYPGQQEKCFVCGQTGHFASDCPGKSGSNNAAADIPIHKKKYQFLNIWVLREYLQYELAIPDPPFMINFERIIDDFVFLCFFVGNDFLPHMPTLEIREGAINLLMHVYRKEFTAMGGYLTDSGEVLLDRVEHFIQAVAVNEDKIFQKRTRIKQSMDNNEEMKQRSRRDPSEVPPEPIDDKIKLGEPGYKERYYAEKFSTTNPEETEQIKQDMVLKYVEGLCWVCRYYYQGVCSWQWFYPYHYAPFASDLKNLPDLEITFFIGEPFKPFDQLMGTLPAASSNALPGEYRKLMTDPSSPILKFYPADFELDMNGKRFAWQGIAKLPFIEEKLLLAATRKLEETLTVEEQQRNSVMLDLLYVHPAHPLGQRILQYYHFYQHMPPHECLPWMIDPNSSQGMNGFLWFSERNGFQTRVDSPVNGLPCIEQNRALNVTYLCPAKHSHISEPPRGAIIPDKILTSVDIKPFPPLWHEDNSNRRRQARDRPQVVGAIAGPSLGEAAHRLIKNTLNMKSSTGAASGLIDPNGYYRNVPGNYSYGGVNRPRAPGPSPYRKAYDDDSSYYYGKYNNSTQGTFNNGPRYPYPSNGSQDYNRNYNSKIVAEQHNRGGLGAGMSGLSIEDNGRSKQLYSSYTEAANANLNPLPSPPTQWIGTQPGGNFVGGYYRDGVGYSETNGKSVKKVIYQAKTQPSHRGANL

Target ORF information:

RefSeq Version	NM_104327.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana exoribonuclease 4 (XRN4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_104327.4

ORF Insert Sequence:

ATGGGAGTAC CGGCGTTCTA CAGATGGCTC GCTGACCGAT ACCCGAAGTC AATTAGTGAC 
GTCGTTGAAG AAGAACCTAC CGATGGAGGT CGTGGTGACC TTATTCCTGT TGATATCACC 
AGACCTAACC CTAACGGCTT CGAATTTGAT AATTTGTATC TCGATATGAA TGGTATCATT 
CATCCTTGTT TCCATCCTGA AGGAAAGCCT GCACCTGCCA CGTACGATGA TGTATTTAAA 
TCAATGTTTG AGTACATTGA CCATCTTTTT ACTTTAGTGC GTCCGAGGAA GATTCTTTAT 
TTGGCTATAG ATGGAGTTGC GCCGAGAGCA AAGATGAATC AGCAGCGTTC TCGACGTTTC 
AGAGCTGCAA AGGACGCAGC AGAGGCTGAA GCTGAGGAAG AGAGGTTGAG AAAAGATTTT 
GAGATGGAGG GCCAAATCTT GTCCGCTAAA GAGAAAGCTG AAACCTGTGA TTCAAATGTC 
ATTACTCCTG GGACCCCTTT TATGGCTATA CTGTCAGTCG CTCTTCAGTA TTACATCCAA 
TCTAGGTTAA ACCATAACCC TGGCTGGAGA TATGTTAAGG TAATTCTTTC TGATTCAAAT 
GTTCCGGGAG AAGGAGAACA CAAGATAATG TCATACATTC GTCTCCAACG TAATCTTCCT 
GGTTTTGATC CAAATACACG GCATTGTCTA TATGGTCTGG ATGCAGATTT GATAATGCTC 
TCCTTAGCTA CACATGAAGT TCACTTTTCC ATCTTAAGAG AGGTTATCAC TTACCCTGGG 
CAGCAAGAGA AATGCTTTGT GTGTGGACAA ACTGGCCACT TTGCTTCAGA TTGCCCTGGA 
AAATCAGGTA GCAATAATGC AGCAGCTGAT ATTCCAATCC ACAAGAAAAA ATATCAGTTC 
CTGAACATTT GGGTGTTGCG AGAATATCTG CAATATGAAT TGGCCATTCC AGATCCTCCT 
TTTATGATCA ACTTTGAGAG AATAATAGAC GACTTTGTAT TCCTTTGCTT TTTTGTTGGT 
AATGACTTCC TACCTCATAT GCCTACACTG GAAATTCGAG AGGGGGCTAT AAACTTGCTG 
ATGCATGTTT ACAGAAAGGA GTTTACAGCT ATGGGAGGCT ATCTAACAGA TTCTGGTGAG 
GTTTTACTAG ATAGGGTAGA GCATTTCATT CAAGCTGTCG CTGTAAATGA AGATAAAATA 
TTTCAGAAAA GAACGCGGAT AAAACAGTCA ATGGATAACA ATGAAGAAAT GAAGCAAAGA 
TCTAGAAGGG ACCCATCTGA GGTACCACCT GAACCGATAG ACGATAAGAT TAAATTAGGA 
GAACCAGGCT ACAAGGAGAG GTATTATGCT GAGAAATTTA GTACGACCAA TCCAGAGGAA 
ACTGAGCAAA TCAAACAAGA CATGGTACTG AAATATGTTG AAGGTTTGTG CTGGGTTTGC 
CGGTACTACT ACCAAGGTGT ATGCTCTTGG CAATGGTTTT ACCCATACCA TTATGCTCCA 
TTTGCTTCAG ATCTTAAGAA TCTACCTGAT TTAGAAATCA CATTTTTTAT TGGAGAGCCC 
TTTAAACCTT TTGACCAGTT AATGGGAACC CTGCCGGCTG CAAGCTCAAA TGCGCTGCCT 
GGAGAATACC GGAAGTTGAT GACTGATCCC TCATCCCCGA TACTTAAATT TTACCCTGCT 
GATTTTGAGC TTGACATGAA TGGAAAGCGC TTCGCCTGGC AGGGTATTGC AAAACTTCCT 
TTCATTGAGG AGAAATTATT GTTAGCTGCT ACAAGGAAGC TTGAAGAAAC TCTAACGGTG 
GAAGAACAAC AACGCAACAG TGTGATGCTT GATTTGTTAT ACGTACACCC CGCTCATCCA 
CTGGGCCAGC GGATTCTGCA GTACTATCAT TTCTACCAGC ACATGCCACC ACATGAATGT 
CTTCCATGGA TGATTGACCC GAATTCTAGC CAAGGAATGA ACGGTTTTCT TTGGTTTAGT 
GAAAGGAATG GTTTCCAAAC TAGAGTGGAT TCCCCTGTCA ATGGTTTGCC ATGCATTGAG 
CAGAACCGAG CCTTAAATGT TACATACCTG TGTCCTGCCA AACACTCTCA TATCTCAGAA 
CCACCCAGAG GAGCGATCAT TCCGGACAAG ATCTTGACGT CAGTAGATAT AAAGCCATTT 
CCTCCTTTAT GGCATGAAGA CAATAGCAAC CGACGCCGAC AAGCCCGAGA TAGGCCGCAA 
GTGGTTGGAG CTATAGCAGG GCCTTCTCTG GGAGAAGCAG CTCATCGTCT GATCAAAAAC 
ACCCTTAACA TGAAATCCTC TACTGGTGCG GCTTCGGGAT TAATTGACCC AAACGGATAC 
TACCGAAATG TACCGGGTAA TTATTCTTAT GGTGGAGTCA ATAGACCAAG AGCACCGGGT 
CCGTCTCCTT ACCGGAAAGC TTATGACGAT GACTCGAGCT ATTACTATGG AAAGTATAAC 
AATAGCACAC AGGGAACGTT TAACAATGGT CCAAGATATC CTTATCCATC AAATGGGTCA 
CAAGACTATA ACCGGAACTA CAACTCCAAG ATAGTAGCAG AACAGCACAA CCGTGGTGGA 
CTTGGAGCTG GAATGTCTGG TTTATCGATA GAAGATAACG GCAGAAGCAA ACAATTGTAT 
TCAAGTTATA CAGAAGCTGC TAATGCAAAT TTGAACCCGT TGCCATCACC CCCCACCCAA 
TGGATCGGTA CACAGCCAGG TGGTAATTTT GTCGGTGGAT ACTATAGGGA TGGAGTTGGG 
TACAGTGAAA CAAATGGAAA ATCGGTAAAG AAAGTAATTT ACCAGGCCAA GACACAACCA 
AGTCATCGAG GTGCAAACTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

XRN4 ( NM_001333652.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001321327.1 Arabidopsis thaliana exoribonuclease 4 (XRN4), mRNA.

Price & Availability↑

CloneID	OAb34443
Clone ID Related Accession (Same CDS sequence)	NM_001333652.1
Accession Version	NM_001333652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	exoribonuclease 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGAAGCGA TGGCCAACTT GGTGATACTA GACCCTGCAC CTGCCACGTA CGATGATGTA 
TTTAAATCAA TGTTTGAGTA CATTGACCAT CTTTTTACTT TAGTGCGTCC GAGGAAGATT 
CTTTATTTGG CTATAGATGG AGTTGCGCCG AGAGCAAAGA TGAATCAGCA GCGTTCTCGA 
CGTTTCAGAG CTGCAAAGGA CGCAGCAGAG GCTGAAGCTG AGGAAGAGAG GTTGAGAAAA 
GATTTTGAGA TGGAGGGCCA AATCTTGTCC GCTAAAGAGA AAGCTGAAAC CTGTGATTCA 
AATGTCATTA CTCCTGGGAC CCCTTTTATG GCTATACTGT CAGTCGCTCT TCAGTATTAC 
ATCCAATCTA GGTTAAACCA TAACCCTGGC TGGAGATATG TTAAGGTAAT TCTTTCTGAT 
TCAAATGTTC CGGGAGAAGG AGAACACAAG ATAATGTCAT ACATTCGTCT CCAACGTAAT 
CTTCCTGGTT TTGATCCAAA TACACGGCAT TGTCTATATG GTCTGGATGC AGATTTGATA 
ATGCTCTCCT TAGCTACACA TGAAGTTCAC TTTTCCATCT TAAGAGAGGT TATCACTTAC 
CCTGGGCAGC AAGAGAAATG CTTTGTGTGT GGACAAACTG GCCACTTTGC TTCAGATTGC 
CCTGGAAAAT CAGGTAGCAA TAATGCAGCA GCTGATATTC CAATCCACAA GAAAAAATAT 
CAGTTCCTGA ACATTTGGGT GTTGCGAGAA TATCTGCAAT ATGAATTGGC CATTCCAGAT 
CCTCCTTTTA TGATCAACTT TGAGAGAATA ATAGACGACT TTGTATTCCT TTGCTTTTTT 
GTTGGTAATG ACTTCCTACC TCATATGCCT ACACTGGAAA TTCGAGAGGG GGCTATAAAC 
TTGCTGATGC ATGTTTACAG AAAGGAGTTT ACAGCTATGG GAGGCTATCT AACAGATTCT 
GGTGAGGTTT TACTAGATAG GGTAGAGCAT TTCATTCAAG CTGTCGCTGT AAATGAAGAT 
AAAATATTTC AGAAAAGAAC GCGGATAAAA CAGTCAATGG ATAACAATGA AGAAATGAAG 
CAAAGATCTA GAAGGGACCC ATCTGAGGTA CCACCTGAAC CGATAGACGA TAAGATTAAA 
TTAGGAGAAC CAGGCTACAA GGAGAGGTAT TATGCTGAGA AATTTAGTAC GACCAATCCA 
GAGGAAACTG AGCAAATCAA ACAAGACATG GTACTGAAAT ATGTTGAAGG TTTGTGCTGG 
GTTTGCCGGT ACTACTACCA AGGTGTATGC TCTTGGCAAT GGTTTTACCC ATACCATTAT 
GCTCCATTTG CTTCAGATCT TAAGAATCTA CCTGATTTAG AAATCACATT TTTTATTGGA 
GAGCCCTTTA AACCTTTTGA CCAGTTAATG GGAACCCTGC CGGCTGCAAG CTCAAATGCG 
CTGCCTGGAG AATACCGGAA GTTGATGACT GATCCCTCAT CCCCGATACT TAAATTTTAC 
CCTGCTGATT TTGAGCTTGA CATGAATGGA AAGCGCTTCG CCTGGCAGGG TATTGCAAAA 
CTTCCTTTCA TTGAGGAGAA ATTATTGTTA GCTGCTACAA GGAAGCTTGA AGAAACTCTA 
ACGGTGGAAG AACAACAACG CAACAGTGTG ATGCTTGATT TGTTATACGT ACACCCCGCT 
CATCCACTGG GCCAGCGGAT TCTGCAGTAC TATCATTTCT ACCAGCACAT GCCACCACAT 
GAATGTCTTC CATGGATGAT TGACCCGAAT TCTAGCCAAG GAATGAACGG TTTTCTTTGG 
TTTAGTGAAA GGAATGGTTT CCAAACTAGA GTGGATTCCC CTGTCAATGG TTTGCCATGC 
ATTGAGCAGA ACCGAGCCTT AAATGTTACA TACCTGTGTC CTGCCAAACA CTCTCATATC 
TCAGAACCAC CCAGAGGAGC GATCATTCCG GACAAGATCT TGACGTCAGT AGATATAAAG 
CCATTTCCTC CTTTATGGCA TGAAGACAAT AGCAACCGAC GCCGACAAGC CCGAGATAGG 
CCGCAAGTGG TTGGAGCTAT AGCAGGGCCT TCTCTGGGAG AAGCAGCTCA TCGTCTGATC 
AAAAACACCC TTAACATGAA ATCCTCTACT GGTGCGGCTT CGGGATTAAT TGACCCAAAC 
GGATACTACC GAAATGTACC GGGTAATTAT TCTTATGGTG GAGTCAATAG ACCAAGAGCA 
CCGGGTCCGT CTCCTTACCG GAAAGCTTAT GACGATGACT CGAGCTATTA CTATGGAAAG 
TATAACAATA GCACACAGGG AACGTTTAAC AATGGTCCAA GATATCCTTA TCCATCAAAT 
GGGTCACAAG ACTATAACCG GAACTACAAC TCCAAGATAG TAGCAGAACA GCACAACCGT 
GGTGGACTTG GAGCTGGAAT GTCTGGTTTA TCGATAGAAG ATAACGGCAG AAGCAAACAA 
TTGTATTCAA GTTATACAGA AGCTGCTAAT GCAAATTTGA ACCCGTTGCC ATCACCCCCC 
ACCCAATGGA TCGGTACACA GCCAGGTGGT AATTTTGTCG GTGGATACTA TAGGGATGGA 
GTTGGGTACA GTGAAACAAA TGGAAAATCG GTAAAGAAAG TAATTTACCA GGCCAAGACA 
CAACCAAGTC ATCGAGGTGC AAACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001321327.1
CDS	571..3240
Translation	MEAMANLVILDPAPATYDDVFKSMFEYIDHLFTLVRPRKILYLAIDGVAPRAKMNQQRSRRFRAAKDAAEAEAEEERLRKDFEMEGQILSAKEKAETCDSNVITPGTPFMAILSVALQYYIQSRLNHNPGWRYVKVILSDSNVPGEGEHKIMSYIRLQRNLPGFDPNTRHCLYGLDADLIMLSLATHEVHFSILREVITYPGQQEKCFVCGQTGHFASDCPGKSGSNNAAADIPIHKKKYQFLNIWVLREYLQYELAIPDPPFMINFERIIDDFVFLCFFVGNDFLPHMPTLEIREGAINLLMHVYRKEFTAMGGYLTDSGEVLLDRVEHFIQAVAVNEDKIFQKRTRIKQSMDNNEEMKQRSRRDPSEVPPEPIDDKIKLGEPGYKERYYAEKFSTTNPEETEQIKQDMVLKYVEGLCWVCRYYYQGVCSWQWFYPYHYAPFASDLKNLPDLEITFFIGEPFKPFDQLMGTLPAASSNALPGEYRKLMTDPSSPILKFYPADFELDMNGKRFAWQGIAKLPFIEEKLLLAATRKLEETLTVEEQQRNSVMLDLLYVHPAHPLGQRILQYYHFYQHMPPHECLPWMIDPNSSQGMNGFLWFSERNGFQTRVDSPVNGLPCIEQNRALNVTYLCPAKHSHISEPPRGAIIPDKILTSVDIKPFPPLWHEDNSNRRRQARDRPQVVGAIAGPSLGEAAHRLIKNTLNMKSSTGAASGLIDPNGYYRNVPGNYSYGGVNRPRAPGPSPYRKAYDDDSSYYYGKYNNSTQGTFNNGPRYPYPSNGSQDYNRNYNSKIVAEQHNRGGLGAGMSGLSIEDNGRSKQLYSSYTEAANANLNPLPSPPTQWIGTQPGGNFVGGYYRDGVGYSETNGKSVKKVIYQAKTQPSHRGANL

Target ORF information:

RefSeq Version	NM_001333652.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana exoribonuclease 4 (XRN4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001333652.1

ORF Insert Sequence:

ATGGAAGCGA TGGCCAACTT GGTGATACTA GACCCTGCAC CTGCCACGTA CGATGATGTA 
TTTAAATCAA TGTTTGAGTA CATTGACCAT CTTTTTACTT TAGTGCGTCC GAGGAAGATT 
CTTTATTTGG CTATAGATGG AGTTGCGCCG AGAGCAAAGA TGAATCAGCA GCGTTCTCGA 
CGTTTCAGAG CTGCAAAGGA CGCAGCAGAG GCTGAAGCTG AGGAAGAGAG GTTGAGAAAA 
GATTTTGAGA TGGAGGGCCA AATCTTGTCC GCTAAAGAGA AAGCTGAAAC CTGTGATTCA 
AATGTCATTA CTCCTGGGAC CCCTTTTATG GCTATACTGT CAGTCGCTCT TCAGTATTAC 
ATCCAATCTA GGTTAAACCA TAACCCTGGC TGGAGATATG TTAAGGTAAT TCTTTCTGAT 
TCAAATGTTC CGGGAGAAGG AGAACACAAG ATAATGTCAT ACATTCGTCT CCAACGTAAT 
CTTCCTGGTT TTGATCCAAA TACACGGCAT TGTCTATATG GTCTGGATGC AGATTTGATA 
ATGCTCTCCT TAGCTACACA TGAAGTTCAC TTTTCCATCT TAAGAGAGGT TATCACTTAC 
CCTGGGCAGC AAGAGAAATG CTTTGTGTGT GGACAAACTG GCCACTTTGC TTCAGATTGC 
CCTGGAAAAT CAGGTAGCAA TAATGCAGCA GCTGATATTC CAATCCACAA GAAAAAATAT 
CAGTTCCTGA ACATTTGGGT GTTGCGAGAA TATCTGCAAT ATGAATTGGC CATTCCAGAT 
CCTCCTTTTA TGATCAACTT TGAGAGAATA ATAGACGACT TTGTATTCCT TTGCTTTTTT 
GTTGGTAATG ACTTCCTACC TCATATGCCT ACACTGGAAA TTCGAGAGGG GGCTATAAAC 
TTGCTGATGC ATGTTTACAG AAAGGAGTTT ACAGCTATGG GAGGCTATCT AACAGATTCT 
GGTGAGGTTT TACTAGATAG GGTAGAGCAT TTCATTCAAG CTGTCGCTGT AAATGAAGAT 
AAAATATTTC AGAAAAGAAC GCGGATAAAA CAGTCAATGG ATAACAATGA AGAAATGAAG 
CAAAGATCTA GAAGGGACCC ATCTGAGGTA CCACCTGAAC CGATAGACGA TAAGATTAAA 
TTAGGAGAAC CAGGCTACAA GGAGAGGTAT TATGCTGAGA AATTTAGTAC GACCAATCCA 
GAGGAAACTG AGCAAATCAA ACAAGACATG GTACTGAAAT ATGTTGAAGG TTTGTGCTGG 
GTTTGCCGGT ACTACTACCA AGGTGTATGC TCTTGGCAAT GGTTTTACCC ATACCATTAT 
GCTCCATTTG CTTCAGATCT TAAGAATCTA CCTGATTTAG AAATCACATT TTTTATTGGA 
GAGCCCTTTA AACCTTTTGA CCAGTTAATG GGAACCCTGC CGGCTGCAAG CTCAAATGCG 
CTGCCTGGAG AATACCGGAA GTTGATGACT GATCCCTCAT CCCCGATACT TAAATTTTAC 
CCTGCTGATT TTGAGCTTGA CATGAATGGA AAGCGCTTCG CCTGGCAGGG TATTGCAAAA 
CTTCCTTTCA TTGAGGAGAA ATTATTGTTA GCTGCTACAA GGAAGCTTGA AGAAACTCTA 
ACGGTGGAAG AACAACAACG CAACAGTGTG ATGCTTGATT TGTTATACGT ACACCCCGCT 
CATCCACTGG GCCAGCGGAT TCTGCAGTAC TATCATTTCT ACCAGCACAT GCCACCACAT 
GAATGTCTTC CATGGATGAT TGACCCGAAT TCTAGCCAAG GAATGAACGG TTTTCTTTGG 
TTTAGTGAAA GGAATGGTTT CCAAACTAGA GTGGATTCCC CTGTCAATGG TTTGCCATGC 
ATTGAGCAGA ACCGAGCCTT AAATGTTACA TACCTGTGTC CTGCCAAACA CTCTCATATC 
TCAGAACCAC CCAGAGGAGC GATCATTCCG GACAAGATCT TGACGTCAGT AGATATAAAG 
CCATTTCCTC CTTTATGGCA TGAAGACAAT AGCAACCGAC GCCGACAAGC CCGAGATAGG 
CCGCAAGTGG TTGGAGCTAT AGCAGGGCCT TCTCTGGGAG AAGCAGCTCA TCGTCTGATC 
AAAAACACCC TTAACATGAA ATCCTCTACT GGTGCGGCTT CGGGATTAAT TGACCCAAAC 
GGATACTACC GAAATGTACC GGGTAATTAT TCTTATGGTG GAGTCAATAG ACCAAGAGCA 
CCGGGTCCGT CTCCTTACCG GAAAGCTTAT GACGATGACT CGAGCTATTA CTATGGAAAG 
TATAACAATA GCACACAGGG AACGTTTAAC AATGGTCCAA GATATCCTTA TCCATCAAAT 
GGGTCACAAG ACTATAACCG GAACTACAAC TCCAAGATAG TAGCAGAACA GCACAACCGT 
GGTGGACTTG GAGCTGGAAT GTCTGGTTTA TCGATAGAAG ATAACGGCAG AAGCAAACAA 
TTGTATTCAA GTTATACAGA AGCTGCTAAT GCAAATTTGA ACCCGTTGCC ATCACCCCCC 
ACCCAATGGA TCGGTACACA GCCAGGTGGT AATTTTGTCG GTGGATACTA TAGGGATGGA 
GTTGGGTACA GTGAAACAAA TGGAAAATCG GTAAAGAAAG TAATTTACCA GGCCAAGACA 
CAACCAAGTC ATCGAGGTGC AAACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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XRN4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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