POLGAMMA2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLGAMMA2
Entrez Gene ID	841506
Full Name	polymerase gamma 2
Synonyms	F8A12.8,F8A12_8,PolIA,polymerase I A,polymerase gamma 2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001333457.1	NP_001321058.1	polymerase gamma 2
NM_103965.5	NP_175498.2	polymerase gamma 2

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os08g0175300	NP_001061112.1
Oryza sativa (rice)	Os08g0175600	NP_001061113.1
Arabidopsis thaliana (thale cress)	POLGAMMA2	NP_175498.2
Arabidopsis thaliana (thale cress)	POLGAMMA1	NP_188690.3

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

both AtPolIA and AtPolIB are efficient translesion DNA synthesis polymerases, providing the first example of a family-A DNAP with an active exonuclease domain that efficiently bypasses an AP site.
Title: Plant organellar DNA polymerases are replicative and translesion DNA synthesis polymerases.

Alternative translation initiation in Polgamma2 involves leaky ribosome scanning of both cis- and trans-acting components.
Title: Participation of leaky ribosome scanning in protein dual targeting by alternative translation initiation in higher plants.

The following POLGAMMA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLGAMMA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb34342	NM_001333457.1 Latest version!	Arabidopsis thaliana polymerase gamma 2 (POLGAMMA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAb26036	NM_103965.5 Latest version!	Arabidopsis thaliana polymerase gamma 2 (POLGAMMA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLGAMMA2 ( NM_001333457.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001321058.1 Arabidopsis thaliana polymerase gamma 2 (POLGAMMA2), mRNA.

Price & Availability↑

CloneID	OAb34342
Clone ID Related Accession (Same CDS sequence)	NM_001333457.1
Accession Version	NM_001333457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	polymerase gamma 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGCCATGG GGGTTTCTCT TACTTCCCAC AATAACCCTC TTCTTCGCCA CTTATCTCCT 
TCCTCTTCCT GGGTTTCTCG TTCCTCCTCT CGCCTCTCTT CTTCTCCACT CCCTTCCTTC 
CTCTTTCCTT GCCGTCGAAC CCTTCTTCAA AGGAAACTAG CTTCCACTGA TGGAAATGTC 
GGTTATTGTA CTACTACAGT TTGTCAGGGA TTTCAGCATT CGGTTCATCA ACGGTCGTCT 
TCTGTTGTGT TTAACGGAGA GTGGGAACTT CGATCGGAAT CTAACAAGGT AAGGATGGTT 
CCTAAGATTA TCAAGGTTGG AAATCAGACG GAGGTAGCCG AAACTCATCA AGTTCCTGGA 
ACTGTAAGTG CTTGGAGGGA AGAAGCTAAT AAGCTTAGAG AAAGAAATGG TCAAATTGCT 
AGAAACCTTG ATGACAATGG TTATTTCAAT GGCTCTGTCC CAATTATATC TAGTGCTCCG 
TCTTATGAAA CCTCTCAGAA GATTGATTAT GAGTTCAAGC CCAGAGGGAC CACTAGGTCC 
ACTACTGCTA CACTCAATAA GGAATTGATT GGCATTACCC AATCTGAACC TGTTGTGTCA 
TTGCCAAGGA AGGGTTTAGA TGTTGGGGAT AATATGGATG TAAATCCTAA AGGAGAGGGA 
ATACAGAGAC CTCTTATAAG CGATAAGTCT TCTGGTACCG CAAATGGAAA CAAGAATACT 
GTTGCCATTT CCAAAGTGGA GAGAAGCACT GAACCTTCGA ATGTCCGTGA AAACCTTGGG 
AAAATATATG ACAAGGTTCT CATTGTTGAT AATGTGCAAG CTGCGAAGGA TACCGTGGCT 
AAGCTCGTGA ATCAGTTTAG GAATCATGTC CATTCCTGTG ATACAGAGGT GTCCGGGATT 
GAGGTTAAGG AAGAAACGCC TGTCGACCAC GGTGAATTAA TATGTTTCAG TATTTACTGT 
GGGCCAGAAG CAGATTTTGG GAATGGAAAA TCGTGCATCT GGGTTGATGT TCTTGGTGAA 
AATGGCAGGG AGGTGTTGGC CGAGTTTAAA CCATATTTTG AAGACTCGTT CATCAGAAAA 
GTCTGGCACA ACTACAGTTT CGATAGCCAC ATCATTAGGA ACCATGGAAT TGAGATTTCT 
GGTTTTCATG CCGACACTAT GCACATGGCA CGATTATGGG ATTCTGCACG GCGGATAAAA 
GGTGGTTATT CGCTTGAAGC GCTTACAAGT GACCCAAAAG TTCTTGGGGG CACACAGACA 
AAGGAGGAAG CTGAGTTCCT TGGTAAAATT TCGATGAAGA CTATTTTTGG CAAACGGAAA 
CTGAAAAAAG ATGGATCTGA AGGGAAAATC GTTGTCATTC CCCCTGTTGA AGAGCTTCAG 
CGAGAAGATC GAGAGGCTTG GATTTCATAC TCTGCCTTGG ATGCGATAAG CACACTAAAG 
CTTTACGAGA GCATGACAAA GAAACTGCAA TTGATGGATT GGCATCTTGA TGGAAAGCCA 
GTTTTAGGAA GGACAATGTT GGATTTTTAC CATGAGTTCT GGCGACCCTT TGGTGAACTT 
CTTGTAAAAA TGGAAGCAGA AGGGATACTT GTAGATAGAG AGTATCTTGC TGAGATAGAG 
AAAGTAGCCA AAGCGGAACA ACAAGTTGCT GGTAGTAGGT TTCGTAATTG GGCATCTAAG 
TATTGCCCCG ATGCGAAGTA TATGAATATT GGCAGTGACA CACAATTGCG TCAGCTCTTT 
TTTGGTGGCA TTTCCAACAG TAGTCATGAC GAGGTGCTTC CAGTTGAAAA GCTCTTCAAA 
GTCCCCAATA TTGATAAGGT TATTGAAGAA GGCAAAAAAA CACCGACGAA GTTTCGAAAC 
ATCAAACTGC ATAGGATAAG TGATAGCCCA CTGTCAACGG AAAATTTTAC TGCCAGTGGT 
TGGCCCTCTG TAGGTGGGGA TGTTTTGAAA GAGTTAGCTG GGAAAGTTTC TGCTGAATAC 
GACTTTATGG ATGATGTCTC AGATATTTCT CTGGAAGAAG TTGTTGAGGA TGATGATGTA 
GAAACATCAG AAACACAAAA GTCCAAGACA GATGATGAAA CCGACACATC TGCTTATGGA 
ACAGCATATG TTGCATTTGG AGGGGGTGAA AGGGGAAAGG AGGCATGTCA TGCTATTGCC 
TCATTATGTG AAGTTTGCTC TATAGACTCT TTGATCTCAA ATTTTATTCT TCCGTTACAG 
GGAAGTAATG TATCAGGCAA AGATGGTCGC GTCCATTGCT CCCTTAATAT CAATACAGAA 
ACTGGGCGCT TATCAGCTAG AAGGCCAAAT TTGCAGAACC AACCTGCATT GGAGAAGGAT 
CGGTACAAGA TTCGTAAGGC CTTTGTAGCA TCGCCTGGAA ATACACTTGT TGTGGCTGAT 
TATGGGCAGT TGGAACTTAG AATTCTGGCA CATCTTACTG GTTGTAAAAG CATGATGGAA 
GCTTTTAAAG CAGGCGGAGA TTTCCACTCA AGGACAGCCA TGAATATGTA TCCACATGTT 
CGTGAAGCTG TAGAAAATGG GCAAGTGATC CTTGAATGGC ATCCAGAACC TGGGGAAGAC 
AAGCCACCTG TGCCGTTGTT AAAGGATGCC TTTGGTTCAG AGAGAAGAAA AGCCAAGATG 
CTTAACTTTT CAATTGCGTA TGGGAAAACC GCGGTCGGGC TTTCTAGAGA TTGGAAGGTC 
TCCACTAAAG AAGCTCAGGA AACAGTCGAT CTCTGGTACA ATGACAGGCA AGAAGTAAGG 
AAATGGCAAG AAATGCGTAA GAAAGAAGCT ATAGAAGATG GGTATGTACT CACTTTACTA 
GGAAGGTCTC GTAGATTTCC GGCGTCAAAG TCACGTGCTC AGAGAAATCA TATCCAAAGA 
GCAGCAATCA ACACTCCAGT CCAGGGAAGT GCAGCTGATG TTGCCATGTG TGCAATGTTG 
GAAATATCGA TAAATCAACA GTTGAAGAAG CTTGGTTGGA GATTGCTTCT ACAGATTCAT 
GATGAAGTAA TCTTGGAAGG ACCAATTGAA TCAGCGGAGA TCGCAAAGGA CATAGTTGTG 
GACTGTATGT CTAAGCCCTT TAACGGAAGG AATATTCTCT CAGTCGATTT ATCTGTTGAT 
GCAAAATGTG CTCAAAACTG GTATGCTGCC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001321058.1
CDS	231..3386
Translation	MAMGVSLTSHNNPLLRHLSPSSSWVSRSSSRLSSSPLPSFLFPCRRTLLQRKLASTDGNVGYCTTTVCQGFQHSVHQRSSSVVFNGEWELRSESNKVRMVPKIIKVGNQTEVAETHQVPGTVSAWREEANKLRERNGQIARNLDDNGYFNGSVPIISSAPSYETSQKIDYEFKPRGTTRSTTATLNKELIGITQSEPVVSLPRKGLDVGDNMDVNPKGEGIQRPLISDKSSGTANGNKNTVAISKVERSTEPSNVRENLGKIYDKVLIVDNVQAAKDTVAKLVNQFRNHVHSCDTEVSGIEVKEETPVDHGELICFSIYCGPEADFGNGKSCIWVDVLGENGREVLAEFKPYFEDSFIRKVWHNYSFDSHIIRNHGIEISGFHADTMHMARLWDSARRIKGGYSLEALTSDPKVLGGTQTKEEAEFLGKISMKTIFGKRKLKKDGSEGKIVVIPPVEELQREDREAWISYSALDAISTLKLYESMTKKLQLMDWHLDGKPVLGRTMLDFYHEFWRPFGELLVKMEAEGILVDREYLAEIEKVAKAEQQVAGSRFRNWASKYCPDAKYMNIGSDTQLRQLFFGGISNSSHDEVLPVEKLFKVPNIDKVIEEGKKTPTKFRNIKLHRISDSPLSTENFTASGWPSVGGDVLKELAGKVSAEYDFMDDVSDISLEEVVEDDDVETSETQKSKTDDETDTSAYGTAYVAFGGGERGKEACHAIASLCEVCSIDSLISNFILPLQGSNVSGKDGRVHCSLNINTETGRLSARRPNLQNQPALEKDRYKIRKAFVASPGNTLVVADYGQLELRILAHLTGCKSMMEAFKAGGDFHSRTAMNMYPHVREAVENGQVILEWHPEPGEDKPPVPLLKDAFGSERRKAKMLNFSIAYGKTAVGLSRDWKVSTKEAQETVDLWYNDRQEVRKWQEMRKKEAIEDGYVLTLLGRSRRFPASKSRAQRNHIQRAAINTPVQGSAADVAMCAMLEISINQQLKKLGWRLLLQIHDEVILEGPIESAEIAKDIVVDCMSKPFNGRNILSVDLSVDAKCAQNWYAAK

Target ORF information:

RefSeq Version	NM_001333457.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana polymerase gamma 2 (POLGAMMA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001333457.1

ORF Insert Sequence:

ATGGCCATGG GGGTTTCTCT TACTTCCCAC AATAACCCTC TTCTTCGCCA CTTATCTCCT 
TCCTCTTCCT GGGTTTCTCG TTCCTCCTCT CGCCTCTCTT CTTCTCCACT CCCTTCCTTC 
CTCTTTCCTT GCCGTCGAAC CCTTCTTCAA AGGAAACTAG CTTCCACTGA TGGAAATGTC 
GGTTATTGTA CTACTACAGT TTGTCAGGGA TTTCAGCATT CGGTTCATCA ACGGTCGTCT 
TCTGTTGTGT TTAACGGAGA GTGGGAACTT CGATCGGAAT CTAACAAGGT AAGGATGGTT 
CCTAAGATTA TCAAGGTTGG AAATCAGACG GAGGTAGCCG AAACTCATCA AGTTCCTGGA 
ACTGTAAGTG CTTGGAGGGA AGAAGCTAAT AAGCTTAGAG AAAGAAATGG TCAAATTGCT 
AGAAACCTTG ATGACAATGG TTATTTCAAT GGCTCTGTCC CAATTATATC TAGTGCTCCG 
TCTTATGAAA CCTCTCAGAA GATTGATTAT GAGTTCAAGC CCAGAGGGAC CACTAGGTCC 
ACTACTGCTA CACTCAATAA GGAATTGATT GGCATTACCC AATCTGAACC TGTTGTGTCA 
TTGCCAAGGA AGGGTTTAGA TGTTGGGGAT AATATGGATG TAAATCCTAA AGGAGAGGGA 
ATACAGAGAC CTCTTATAAG CGATAAGTCT TCTGGTACCG CAAATGGAAA CAAGAATACT 
GTTGCCATTT CCAAAGTGGA GAGAAGCACT GAACCTTCGA ATGTCCGTGA AAACCTTGGG 
AAAATATATG ACAAGGTTCT CATTGTTGAT AATGTGCAAG CTGCGAAGGA TACCGTGGCT 
AAGCTCGTGA ATCAGTTTAG GAATCATGTC CATTCCTGTG ATACAGAGGT GTCCGGGATT 
GAGGTTAAGG AAGAAACGCC TGTCGACCAC GGTGAATTAA TATGTTTCAG TATTTACTGT 
GGGCCAGAAG CAGATTTTGG GAATGGAAAA TCGTGCATCT GGGTTGATGT TCTTGGTGAA 
AATGGCAGGG AGGTGTTGGC CGAGTTTAAA CCATATTTTG AAGACTCGTT CATCAGAAAA 
GTCTGGCACA ACTACAGTTT CGATAGCCAC ATCATTAGGA ACCATGGAAT TGAGATTTCT 
GGTTTTCATG CCGACACTAT GCACATGGCA CGATTATGGG ATTCTGCACG GCGGATAAAA 
GGTGGTTATT CGCTTGAAGC GCTTACAAGT GACCCAAAAG TTCTTGGGGG CACACAGACA 
AAGGAGGAAG CTGAGTTCCT TGGTAAAATT TCGATGAAGA CTATTTTTGG CAAACGGAAA 
CTGAAAAAAG ATGGATCTGA AGGGAAAATC GTTGTCATTC CCCCTGTTGA AGAGCTTCAG 
CGAGAAGATC GAGAGGCTTG GATTTCATAC TCTGCCTTGG ATGCGATAAG CACACTAAAG 
CTTTACGAGA GCATGACAAA GAAACTGCAA TTGATGGATT GGCATCTTGA TGGAAAGCCA 
GTTTTAGGAA GGACAATGTT GGATTTTTAC CATGAGTTCT GGCGACCCTT TGGTGAACTT 
CTTGTAAAAA TGGAAGCAGA AGGGATACTT GTAGATAGAG AGTATCTTGC TGAGATAGAG 
AAAGTAGCCA AAGCGGAACA ACAAGTTGCT GGTAGTAGGT TTCGTAATTG GGCATCTAAG 
TATTGCCCCG ATGCGAAGTA TATGAATATT GGCAGTGACA CACAATTGCG TCAGCTCTTT 
TTTGGTGGCA TTTCCAACAG TAGTCATGAC GAGGTGCTTC CAGTTGAAAA GCTCTTCAAA 
GTCCCCAATA TTGATAAGGT TATTGAAGAA GGCAAAAAAA CACCGACGAA GTTTCGAAAC 
ATCAAACTGC ATAGGATAAG TGATAGCCCA CTGTCAACGG AAAATTTTAC TGCCAGTGGT 
TGGCCCTCTG TAGGTGGGGA TGTTTTGAAA GAGTTAGCTG GGAAAGTTTC TGCTGAATAC 
GACTTTATGG ATGATGTCTC AGATATTTCT CTGGAAGAAG TTGTTGAGGA TGATGATGTA 
GAAACATCAG AAACACAAAA GTCCAAGACA GATGATGAAA CCGACACATC TGCTTATGGA 
ACAGCATATG TTGCATTTGG AGGGGGTGAA AGGGGAAAGG AGGCATGTCA TGCTATTGCC 
TCATTATGTG AAGTTTGCTC TATAGACTCT TTGATCTCAA ATTTTATTCT TCCGTTACAG 
GGAAGTAATG TATCAGGCAA AGATGGTCGC GTCCATTGCT CCCTTAATAT CAATACAGAA 
ACTGGGCGCT TATCAGCTAG AAGGCCAAAT TTGCAGAACC AACCTGCATT GGAGAAGGAT 
CGGTACAAGA TTCGTAAGGC CTTTGTAGCA TCGCCTGGAA ATACACTTGT TGTGGCTGAT 
TATGGGCAGT TGGAACTTAG AATTCTGGCA CATCTTACTG GTTGTAAAAG CATGATGGAA 
GCTTTTAAAG CAGGCGGAGA TTTCCACTCA AGGACAGCCA TGAATATGTA TCCACATGTT 
CGTGAAGCTG TAGAAAATGG GCAAGTGATC CTTGAATGGC ATCCAGAACC TGGGGAAGAC 
AAGCCACCTG TGCCGTTGTT AAAGGATGCC TTTGGTTCAG AGAGAAGAAA AGCCAAGATG 
CTTAACTTTT CAATTGCGTA TGGGAAAACC GCGGTCGGGC TTTCTAGAGA TTGGAAGGTC 
TCCACTAAAG AAGCTCAGGA AACAGTCGAT CTCTGGTACA ATGACAGGCA AGAAGTAAGG 
AAATGGCAAG AAATGCGTAA GAAAGAAGCT ATAGAAGATG GGTATGTACT CACTTTACTA 
GGAAGGTCTC GTAGATTTCC GGCGTCAAAG TCACGTGCTC AGAGAAATCA TATCCAAAGA 
GCAGCAATCA ACACTCCAGT CCAGGGAAGT GCAGCTGATG TTGCCATGTG TGCAATGTTG 
GAAATATCGA TAAATCAACA GTTGAAGAAG CTTGGTTGGA GATTGCTTCT ACAGATTCAT 
GATGAAGTAA TCTTGGAAGG ACCAATTGAA TCAGCGGAGA TCGCAAAGGA CATAGTTGTG 
GACTGTATGT CTAAGCCCTT TAACGGAAGG AATATTCTCT CAGTCGATTT ATCTGTTGAT 
GCAAAATGTG CTCAAAACTG GTATGCTGCC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLGAMMA2 ( NM_103965.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_175498.2 Arabidopsis thaliana polymerase gamma 2 (POLGAMMA2), mRNA.

Price & Availability↑

CloneID	OAb26036
Clone ID Related Accession (Same CDS sequence)	NM_103965.5
Accession Version	NM_103965.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	polymerase gamma 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_103965.4.

ATGGCCATGG GGGTTTCTCT TACTTCCCAC AATAACCCTC TTCTTCGCCA CTTATCTCCT 
TCCTCTTCCT GGGTTTCTCG TTCCTCCTCT CGCCTCTCTT CTTCTCCACT CCCTTCCTTC 
CTCTTTCCTT GCCGTCGAAC CCTTCTTCAA AGGAAACTAG CTTCCACTGA TGGAAATGTC 
GGTTATTGTA CTACTACAGT TTGTCAGGGA TTTCAGCATT CGGTTCATCA ACGGTCGTCT 
TCTGTTGTGT TTAACGGAGA GTGGGAACTT CGATCGGAAT CTAACAAGGT AAGGATGGTT 
CCTAAGATTA TCAAGGTTGG AAATCAGACG GAGGTAGCCG AAACTCATCA AGTTCCTGGA 
ACTGTAAGTG CTTGGAGGGA AGAAGCTAAT AAGCTTAGAG AAAGAAATGG TCAAATTGCT 
AGAAACCTTG ATGACAATGG TTATTTCAAT GGCTCTGTCC CAATTATATC TAGTGCTCCG 
TCTTATGAAA CCTCTCAGAA GATTGATTAT GAGTTCAAGC CCAGAGGGAC CACTAGGTCC 
ACTACTGCTA CACTCAATAA GGAATTGATT GGCATTACCC AATCTGAACC TGTTGTGTCA 
TTGCCAAGGA AGGGTTTAGA TGTTGGGGAT AATATGGATG TAAATCCTAA AGGAGAGGGA 
ATACAGAGAC CTCTTATAAG CGATAAGTCT TCTGGTACCG CAAATGGAAA CAAGAATACT 
GTTGCCATTT CCAAAGTGGA GAGAAGCACT GAACCTTCGA ATGTCCGTGA AAACCTTGGG 
AAAATATATG ACAAGGTTCT CATTGTTGAT AATGTGCAAG CTGCGAAGGA TACCGTGGCT 
AAGCTCGTGA ATCAGTTTAG GAATCATGTC CATTCCTGTG ATACAGAGGT GTCCGGGATT 
GAGGTTAAGG AAGAAACGCC TGTCGACCAC GGTGAATTAA TATGTTTCAG TATTTACTGT 
GGGCCAGAAG CAGATTTTGG GAATGGAAAA TCGTGCATCT GGGTTGATGT TCTTGGTGAA 
AATGGCAGGG AGGTGTTGGC CGAGTTTAAA CCATATTTTG AAGACTCGTT CATCAGAAAA 
GTCTGGCACA ACTACAGTTT CGATAGCCAC ATCATTAGGA ACCATGGAAT TGAGATTTCT 
GGTTTTCATG CCGACACTAT GCACATGGCA CGATTATGGG ATTCTGCACG GCGGATAAAA 
GGTGGTTATT CGCTTGAAGC GCTTACAAGT GACCCAAAAG TTCTTGGGGG CACACAGACA 
AAGGAGGAAG CTGAGTTCCT TGGTAAAATT TCGATGAAGA CTATTTTTGG CAAACGGAAA 
CTGAAAAAAG ATGGATCTGA AGGGAAAATC GTTGTCATTC CCCCTGTTGA AGAGCTTCAG 
CGAGAAGATC GAGAGGCTTG GATTTCATAC TCTGCCTTGG ATGCGATAAG CACACTAAAG 
CTTTACGAGA GCATGACAAA GAAACTGCAA TTGATGGATT GGCATCTTGA TGGAAAGCCA 
GTTTTAGGAA GGACAATGTT GGATTTTTAC CATGAGTTCT GGCGACCCTT TGGTGAACTT 
CTTGTAAAAA TGGAAGCAGA AGGGATACTT GTAGATAGAG AGTATCTTGC TGAGATAGAG 
AAAGTAGCCA AAGCGGAACA ACAAGTTGCT GGTAGTAGGT TTCGTAATTG GGCATCTAAG 
TATTGCCCCG ATGCGAAGTA TATGAATATT GGCAGTGACA CACAATTGCG TCAGCTCTTT 
TTTGGTGGCA TTTCCAACAG TCATGACGAG GTGCTTCCAG TTGAAAAGCT CTTCAAAGTC 
CCCAATATTG ATAAGGTTAT TGAAGAAGGC AAAAAAACAC CGACGAAGTT TCGAAACATC 
AAACTGCATA GGATAAGTGA TAGCCCACTG TCAACGGAAA ATTTTACTGC CAGTGGTTGG 
CCCTCTGTAG GTGGGGATGT TTTGAAAGAG TTAGCTGGGA AAGTTTCTGC TGAATACGAC 
TTTATGGATG ATGTCTCAGA TATTTCTCTG GAAGAAGTTG TTGAGGATGA TGATGTAGAA 
ACATCAGAAA CACAAAAGTC CAAGACAGAT GATGAAACCG ACACATCTGC TTATGGAACA 
GCATATGTTG CATTTGGAGG GGGTGAAAGG GGAAAGGAGG CATGTCATGC TATTGCCTCA 
TTATGTGAAG TTTGCTCTAT AGACTCTTTG ATCTCAAATT TTATTCTTCC GTTACAGGGA 
AGTAATGTAT CAGGCAAAGA TGGTCGCGTC CATTGCTCCC TTAATATCAA TACAGAAACT 
GGGCGCTTAT CAGCTAGAAG GCCAAATTTG CAGAACCAAC CTGCATTGGA GAAGGATCGG 
TACAAGATTC GTAAGGCCTT TGTAGCATCG CCTGGAAATA CACTTGTTGT GGCTGATTAT 
GGGCAGTTGG AACTTAGAAT TCTGGCACAT CTTACTGGTT GTAAAAGCAT GATGGAAGCT 
TTTAAAGCAG GCGGAGATTT CCACTCAAGG ACAGCCATGA ATATGTATCC ACATGTTCGT 
GAAGCTGTAG AAAATGGGCA AGTGATCCTT GAATGGCATC CAGAACCTGG GGAAGACAAG 
CCACCTGTGC CGTTGTTAAA GGATGCCTTT GGTTCAGAGA GAAGAAAAGC CAAGATGCTT 
AACTTTTCAA TTGCGTATGG GAAAACCGCG GTCGGGCTTT CTAGAGATTG GAAGGTCTCC 
ACTAAAGAAG CTCAGGAAAC AGTCGATCTC TGGTACAATG ACAGGCAAGA AGTAAGGAAA 
TGGCAAGAAA TGCGTAAGAA AGAAGCTATA GAAGATGGGT ATGTACTCAC TTTACTAGGA 
AGGTCTCGTA GATTTCCGGC GTCAAAGTCA CGTGCTCAGA GAAATCATAT CCAAAGAGCA 
GCAATCAACA CTCCAGTCCA GGGAAGTGCA GCTGATGTTG CCATGTGTGC AATGTTGGAA 
ATATCGATAA ATCAACAGTT GAAGAAGCTT GGTTGGAGAT TGCTTCTACA GATTCATGAT 
GAAGTAATCT TGGAAGGACC AATTGAATCA GCGGAGATCG CAAAGGACAT AGTTGTGGAC 
TGTATGTCTA AGCCCTTTAA CGGAAGGAAT ATTCTCTCAG TCGATTTATC TGTTGATGCA 
AAATGTGCTC AAAACTGGTA TGCTGCCAAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_175498.2
CDS	186..3338
Translation	MAMGVSLTSHNNPLLRHLSPSSSWVSRSSSRLSSSPLPSFLFPCRRTLLQRKLASTDGNVGYCTTTVCQGFQHSVHQRSSSVVFNGEWELRSESNKVRMVPKIIKVGNQTEVAETHQVPGTVSAWREEANKLRERNGQIARNLDDNGYFNGSVPIISSAPSYETSQKIDYEFKPRGTTRSTTATLNKELIGITQSEPVVSLPRKGLDVGDNMDVNPKGEGIQRPLISDKSSGTANGNKNTVAISKVERSTEPSNVRENLGKIYDKVLIVDNVQAAKDTVAKLVNQFRNHVHSCDTEVSGIEVKEETPVDHGELICFSIYCGPEADFGNGKSCIWVDVLGENGREVLAEFKPYFEDSFIRKVWHNYSFDSHIIRNHGIEISGFHADTMHMARLWDSARRIKGGYSLEALTSDPKVLGGTQTKEEAEFLGKISMKTIFGKRKLKKDGSEGKIVVIPPVEELQREDREAWISYSALDAISTLKLYESMTKKLQLMDWHLDGKPVLGRTMLDFYHEFWRPFGELLVKMEAEGILVDREYLAEIEKVAKAEQQVAGSRFRNWASKYCPDAKYMNIGSDTQLRQLFFGGISNSHDEVLPVEKLFKVPNIDKVIEEGKKTPTKFRNIKLHRISDSPLSTENFTASGWPSVGGDVLKELAGKVSAEYDFMDDVSDISLEEVVEDDDVETSETQKSKTDDETDTSAYGTAYVAFGGGERGKEACHAIASLCEVCSIDSLISNFILPLQGSNVSGKDGRVHCSLNINTETGRLSARRPNLQNQPALEKDRYKIRKAFVASPGNTLVVADYGQLELRILAHLTGCKSMMEAFKAGGDFHSRTAMNMYPHVREAVENGQVILEWHPEPGEDKPPVPLLKDAFGSERRKAKMLNFSIAYGKTAVGLSRDWKVSTKEAQETVDLWYNDRQEVRKWQEMRKKEAIEDGYVLTLLGRSRRFPASKSRAQRNHIQRAAINTPVQGSAADVAMCAMLEISINQQLKKLGWRLLLQIHDEVILEGPIESAEIAKDIVVDCMSKPFNGRNILSVDLSVDAKCAQNWYAAK

Target ORF information:

RefSeq Version	NM_103965.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana polymerase gamma 2 (POLGAMMA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_103965.5

ORF Insert Sequence:

ATGGCCATGG GGGTTTCTCT TACTTCCCAC AATAACCCTC TTCTTCGCCA CTTATCTCCT 
TCCTCTTCCT GGGTTTCTCG TTCCTCCTCT CGCCTCTCTT CTTCTCCACT CCCTTCCTTC 
CTCTTTCCTT GCCGTCGAAC CCTTCTTCAA AGGAAACTAG CTTCCACTGA TGGAAATGTC 
GGTTATTGTA CTACTACAGT TTGTCAGGGA TTTCAGCATT CGGTTCATCA ACGGTCGTCT 
TCTGTTGTGT TTAACGGAGA GTGGGAACTT CGATCGGAAT CTAACAAGGT AAGGATGGTT 
CCTAAGATTA TCAAGGTTGG AAATCAGACG GAGGTAGCCG AAACTCATCA AGTTCCTGGA 
ACTGTAAGTG CTTGGAGGGA AGAAGCTAAT AAGCTTAGAG AAAGAAATGG TCAAATTGCT 
AGAAACCTTG ATGACAATGG TTATTTCAAT GGCTCTGTCC CAATTATATC TAGTGCTCCG 
TCTTATGAAA CCTCTCAGAA GATTGATTAT GAGTTCAAGC CCAGAGGGAC CACTAGGTCC 
ACTACTGCTA CACTCAATAA GGAATTGATT GGCATTACCC AATCTGAACC TGTTGTGTCA 
TTGCCAAGGA AGGGTTTAGA TGTTGGGGAT AATATGGATG TAAATCCTAA AGGAGAGGGA 
ATACAGAGAC CTCTTATAAG CGATAAGTCT TCTGGTACCG CAAATGGAAA CAAGAATACT 
GTTGCCATTT CCAAAGTGGA GAGAAGCACT GAACCTTCGA ATGTCCGTGA AAACCTTGGG 
AAAATATATG ACAAGGTTCT CATTGTTGAT AATGTGCAAG CTGCGAAGGA TACCGTGGCT 
AAGCTCGTGA ATCAGTTTAG GAATCATGTC CATTCCTGTG ATACAGAGGT GTCCGGGATT 
GAGGTTAAGG AAGAAACGCC TGTCGACCAC GGTGAATTAA TATGTTTCAG TATTTACTGT 
GGGCCAGAAG CAGATTTTGG GAATGGAAAA TCGTGCATCT GGGTTGATGT TCTTGGTGAA 
AATGGCAGGG AGGTGTTGGC CGAGTTTAAA CCATATTTTG AAGACTCGTT CATCAGAAAA 
GTCTGGCACA ACTACAGTTT CGATAGCCAC ATCATTAGGA ACCATGGAAT TGAGATTTCT 
GGTTTTCATG CCGACACTAT GCACATGGCA CGATTATGGG ATTCTGCACG GCGGATAAAA 
GGTGGTTATT CGCTTGAAGC GCTTACAAGT GACCCAAAAG TTCTTGGGGG CACACAGACA 
AAGGAGGAAG CTGAGTTCCT TGGTAAAATT TCGATGAAGA CTATTTTTGG CAAACGGAAA 
CTGAAAAAAG ATGGATCTGA AGGGAAAATC GTTGTCATTC CCCCTGTTGA AGAGCTTCAG 
CGAGAAGATC GAGAGGCTTG GATTTCATAC TCTGCCTTGG ATGCGATAAG CACACTAAAG 
CTTTACGAGA GCATGACAAA GAAACTGCAA TTGATGGATT GGCATCTTGA TGGAAAGCCA 
GTTTTAGGAA GGACAATGTT GGATTTTTAC CATGAGTTCT GGCGACCCTT TGGTGAACTT 
CTTGTAAAAA TGGAAGCAGA AGGGATACTT GTAGATAGAG AGTATCTTGC TGAGATAGAG 
AAAGTAGCCA AAGCGGAACA ACAAGTTGCT GGTAGTAGGT TTCGTAATTG GGCATCTAAG 
TATTGCCCCG ATGCGAAGTA TATGAATATT GGCAGTGACA CACAATTGCG TCAGCTCTTT 
TTTGGTGGCA TTTCCAACAG TCATGACGAG GTGCTTCCAG TTGAAAAGCT CTTCAAAGTC 
CCCAATATTG ATAAGGTTAT TGAAGAAGGC AAAAAAACAC CGACGAAGTT TCGAAACATC 
AAACTGCATA GGATAAGTGA TAGCCCACTG TCAACGGAAA ATTTTACTGC CAGTGGTTGG 
CCCTCTGTAG GTGGGGATGT TTTGAAAGAG TTAGCTGGGA AAGTTTCTGC TGAATACGAC 
TTTATGGATG ATGTCTCAGA TATTTCTCTG GAAGAAGTTG TTGAGGATGA TGATGTAGAA 
ACATCAGAAA CACAAAAGTC CAAGACAGAT GATGAAACCG ACACATCTGC TTATGGAACA 
GCATATGTTG CATTTGGAGG GGGTGAAAGG GGAAAGGAGG CATGTCATGC TATTGCCTCA 
TTATGTGAAG TTTGCTCTAT AGACTCTTTG ATCTCAAATT TTATTCTTCC GTTACAGGGA 
AGTAATGTAT CAGGCAAAGA TGGTCGCGTC CATTGCTCCC TTAATATCAA TACAGAAACT 
GGGCGCTTAT CAGCTAGAAG GCCAAATTTG CAGAACCAAC CTGCATTGGA GAAGGATCGG 
TACAAGATTC GTAAGGCCTT TGTAGCATCG CCTGGAAATA CACTTGTTGT GGCTGATTAT 
GGGCAGTTGG AACTTAGAAT TCTGGCACAT CTTACTGGTT GTAAAAGCAT GATGGAAGCT 
TTTAAAGCAG GCGGAGATTT CCACTCAAGG ACAGCCATGA ATATGTATCC ACATGTTCGT 
GAAGCTGTAG AAAATGGGCA AGTGATCCTT GAATGGCATC CAGAACCTGG GGAAGACAAG 
CCACCTGTGC CGTTGTTAAA GGATGCCTTT GGTTCAGAGA GAAGAAAAGC CAAGATGCTT 
AACTTTTCAA TTGCGTATGG GAAAACCGCG GTCGGGCTTT CTAGAGATTG GAAGGTCTCC 
ACTAAAGAAG CTCAGGAAAC AGTCGATCTC TGGTACAATG ACAGGCAAGA AGTAAGGAAA 
TGGCAAGAAA TGCGTAAGAA AGAAGCTATA GAAGATGGGT ATGTACTCAC TTTACTAGGA 
AGGTCTCGTA GATTTCCGGC GTCAAAGTCA CGTGCTCAGA GAAATCATAT CCAAAGAGCA 
GCAATCAACA CTCCAGTCCA GGGAAGTGCA GCTGATGTTG CCATGTGTGC AATGTTGGAA 
ATATCGATAA ATCAACAGTT GAAGAAGCTT GGTTGGAGAT TGCTTCTACA GATTCATGAT 
GAAGTAATCT TGGAAGGACC AATTGAATCA GCGGAGATCG CAAAGGACAT AGTTGTGGAC 
TGTATGTCTA AGCCCTTTAA CGGAAGGAAT ATTCTCTCAG TCGATTTATC TGTTGATGCA 
AAATGTGCTC AAAACTGGTA TGCTGCCAAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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POLGAMMA2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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