AGO1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGO1
Entrez Gene ID	841262
Full Name	Stabilizer of iron transporter SufD / Polynucleotidyl transferase
Synonyms	ARGONAUTE 1,AtAGO1,ICU9,T1N15.2,T1N15_2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_179453.3	NP_849784.1	Stabilizer of iron transporter SufD / Polynucleotidyl transferase
NM_103737.4	NP_175274.1	Stabilizer of iron transporter SufD / Polynucleotidyl transferase
NM_001198240.1	NP_001185169.1	Stabilizer of iron transporter SufD / Polynucleotidyl transferase

Pathways from BioSystems

Homologies

Homo sapiens (human)	AGO3	NP_079128.2
Canis lupus familiaris (dog)	AGO3	XP_005629013.1
Danio rerio (zebrafish)	ago3b	NP_001153500.1
Bos taurus (cattle)	AGO3	NP_001001133.1
Gallus gallus (chicken)	AGO3	NP_001026071.1
Arabidopsis thaliana (thale cress)	AGO1	NP_175274.1
Oryza sativa (rice)	Os02g0672200	NP_001047704.1
Xenopus tropicalis (tropical clawed frog)	ago3	XP_002936270.2
Pan troglodytes (chimpanzee)	EIF2C3	XP_001167675.1
Mus musculus (house mouse)	Ago3	NP_700451.2
Rattus norvegicus (Norway rat)	Ago3	NP_001258122.1
	EIF2C3	XP_002802361.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sweet potato mild mottle virus P1 interacts with AGO1 and AGO2, but solely interferes with AGO1 function.
Title: A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC.

Results indicate that TRN1 positively regulates miRNA activity by promoting the association of miRNAs with AGO1, and they reveal opposing roles of two importin beta family proteins in miRNA loading.
Title: TRANSPORTIN1 Promotes the Association of MicroRNA with ARGONAUTE1 in Arabidopsis.

Results suggests that ARGONAUTE1 (AGO1) interacts with chromatin at microRNAs MIR161 and MIR173 loci and causes the disassembly of the transcriptional complex, releasing short and unpolyadenylated transcripts.
Title: Salt Stress Reveals a New Role for ARGONAUTE1 in miRNA Biogenesis at the Transcriptional and Posttranscriptional Levels.

that AGO1 may have functions in gene regulation independent of small RNAs.
Title: mRNA Decay of Most Arabidopsis miRNA Targets Requires Slicer Activity of AGO1.

miR168 is a key miRNA because it regulates the expression of the slicer protein ARGONAUTE1 (AGO1), which catalyzes mRNA cleavage. Interestingly, plant miR168s are highly evolutionarily conserved; however, it is unclear whether MIR168 promoter elements and expression patterns are also conserved.
Title: Comparative analysis of MIR168 promoters in three plant species.

The following AGO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb25829	NM_103737.4 Latest version!	Arabidopsis thaliana Stabilizer of iron transporter SufD / Polynucleotidyl transferase (AGO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb25830	NM_001198240.1 Latest version!	Arabidopsis thaliana Stabilizer of iron transporter SufD / Polynucleotidyl transferase (AGO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb25830	NM_179453.3 Latest version!	Arabidopsis thaliana Stabilizer of iron transporter SufD / Polynucleotidyl transferase (AGO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGO1 ( NM_103737.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_175274.1 Arabidopsis thaliana Stabilizer of iron transporter SufD / Polynucleotidyl transferase (AGO1), mRNA.

Price & Availability↑

CloneID	OAb25829
Clone ID Related Accession (Same CDS sequence)	NM_103737.4
Accession Version	NM_103737.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Stabilizer of iron transporter SufD / Polynucleotidyl transferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_103737.3.

ATGGTGAGAA AGAGAAGAAC GGATGCTCCA TCTGAAGGAG GTGAAGGCTC TGGGTCTCGT 
GAAGCTGGTC CAGTCTCAGG TGGTGGACGT GGTTCACAGC GAGGTGGTTT CCAGCAGGGA 
GGAGGACAAC ACCAAGGTGG AAGGGGTTAT ACTCCTCAAC CTCAACAGGG AGGTCGTGGT 
GGTCGTGGAT ATGGGCAACC ACCACAACAG CAACAACAGT ATGGAGGACC ACAAGAGTAC 
CAAGGAAGAG GAAGAGGAGG ACCTCCTCAT CAAGGAGGTC GAGGAGGGTA TGGCGGTGGC 
CGTGGAGGTG GACCTTCTTC TGGACCACCG CAGAGACAAT CAGTTCCCGA GCTGCATCAA 
GCTACCTCAC CTACTTATCA AGCGGTGTCT TCTCAGCCTA CACTGTCTGA GGTGAGTCCT 
ACCCAGGTAC CAGAACCTAC TGTTCTGGCT CAGCAATTTG AACAACTCTC TGTTGAACAA 
GGAGCTCCCA GTCAGGCAAT CCAGCCTATA CCTTCTTCTA GCAAGGCTTT CAAGTTTCCA 
ATGAGGCCTG GTAAAGGACA GAGTGGAAAG CGTTGCATTG TGAAGGCTAA CCATTTCTTT 
GCTGAACTGC CTGATAAGGA TTTGCACCAT TATGATGTTA CCATTACTCC GGAAGTTACA 
TCAAGGGGTG TCAATCGTGC TGTGATGAAA CAACTTGTTG ATAATTATCG TGATTCTCAC 
CTTGGAAGTC GTCTTCCAGC GTATGATGGT CGAAAAAGTC TTTACACTGC TGGTCCACTT 
CCCTTTAACT CCAAGGAGTT CAGAATCAAT CTTCTTGACG AAGAAGTAGG GGCTGGAGGT 
CAAAGACGAG AAAGGGAATT TAAAGTTGTG ATCAAGCTAG TTGCACGTGC TGATCTGCAT 
CACCTAGGAA TGTTTTTGGA GGGGAAACAA TCAGATGCCC CACAGGAAGC TCTGCAGGTT 
CTTGACATTG TTCTTCGTGA GCTGCCGACC TCTAGGTATA TTCCGGTGGG CCGGTCCTTT 
TATTCCCCTG ATATAGGAAA AAAACAATCA TTGGGGGATG GCTTGGAGAG CTGGCGTGGA 
TTCTACCAAA GCATTCGTCC TACACAGATG GGCTTATCAC TCAATATTGA TATGTCATCG 
ACAGCCTTCA TAGAGGCAAA CCCTGTGATT CAGTTTGTCT GTGATTTGCT TAACCGGGAT 
ATTTCTTCTC GACCTTTATC TGATGCTGAT CGTGTTAAGA TAAAAAAGGC TCTTAGAGGT 
GTCAAAGTTG AAGTGACTCA TCGAGGAAAC ATGCGCCGGA AGTACCGCAT TTCCGGTTTG 
ACTGCTGTGG CCACTCGGGA ATTGACATTC CCAGTAGATG AAAGAAATAC TCAGAAATCT 
GTTGTAGAAT ACTTCCACGA AACATATGGT TTTCGCATTC AGCACACTCA ACTACCATGC 
TTGCAAGTTG GGAATTCTAA TAGGCCTAAT TACTTACCAA TGGAGGTATG CAAGATTGTT 
GAAGGCCAGC GGTATTCCAA AAGATTGAAT GAGAGACAGA TCACTGCTTT GCTGAAGGTT 
ACCTGTCAGC GCCCGATAGA TCGAGAAAAA GATATCTTAC AGACGGTGCA ACTCAATGAT 
TATGCTAAAG ATAATTATGC TCAAGAGTTT GGCATCAAAA TAAGTACTTC TCTGGCTTCT 
GTTGAGGCTC GTATACTGCC TCCTCCATGG CTTAAGTACC ACGAGTCTGG AAGGGAAGGG 
ACTTGTCTGC CACAAGTTGG TCAATGGAAC ATGATGAATA AGAAAATGAT CAATGGTGGA 
ACGGTGAATA ATTGGATCTG CATCAACTTT TCTAGGCAAG TGCAGGACAA TCTAGCGCGT 
ACATTTTGTC AGGAACTTGC TCAAATGTGT TACGTATCTG GCATGGCATT TAATCCGGAA 
CCAGTCCTCC CACCAGTCAG TGCTCGCCCT GAGCAAGTAG AGAAGGTCTT GAAGACTAGA 
TATCATGATG CCACATCAAA ACTCTCCCAA GGAAAAGAAA TTGATCTGCT TATTGTCATT 
CTGCCCGATA ATAATGGATC ATTATACGGT GATTTGAAAC GCATATGTGA GACTGAACTC 
GGCATAGTCT CTCAATGTTG CCTGACAAAG CATGTCTTTA AGATGAGCAA ACAATACATG 
GCTAATGTTG CGCTGAAGAT TAATGTGAAG GTTGGAGGAA GAAACACAGT GCTTGTTGAT 
GCTCTATCTA GGCGGATTCC TCTAGTCAGT GATCGACCCA CCATTATATT TGGTGCTGAT 
GTTACCCACC CTCACCCTGG AGAGGATTCA AGCCCATCTA TTGCTGCTGT TGTGGCATCT 
CAGGATTGGC CTGAAATCAC TAAATATGCT GGATTAGTTT GCGCTCAAGC GCATAGGCAG 
GAGCTCATTC AGGATCTGTT CAAAGAGTGG AAGGATCCTC AGAAAGGTGT GGTGACTGGT 
GGCATGATAA AGGAGTTGCT CATAGCCTTC CGTAGATCAA CTGGGCATAA ACCACTAAGG 
ATCATCTTCT ACAGGGATGG AGTCAGTGAG GGACAATTTT ACCAAGTTTT GCTCTATGAA 
CTTGATGCCA TCCGCAAGGC CTGTGCTTCG CTGGAAGCAG GTTATCAACC ACCAGTGACA 
TTTGTGGTGG TGCAGAAGCG TCATCACACG AGGCTGTTTG CTCAGAACCA CAATGATCGC 
CATTCGGTGG ACAGAAGTGG GAATATTTTA CCTGGCACTG TTGTGGACTC TAAAATCTGC 
CACCCTACAG AGTTTGACTT TTACCTCTGT AGTCATGCTG GTATTCAGGG CACTTCTCGA 
CCTGCTCATT ACCACGTTCT TTGGGATGAG AACAACTTTA CTGCAGATGG ACTTCAATCT 
CTGACCAATA ACTTATGTTA CACGTATGCA AGATGCACAC GCTCAGTTTC AATTGTTCCC 
CCTGCATATT ATGCACATCT AGCAGCTTTT AGGGCTCGAT TCTACATGGA GCCAGAGACA 
TCAGACAGTG GCTCAATGGC TAGTGGGAGC ATGGCACGTG GAGGTGGAAT GGCTGGTAGA 
AGCACACGCG GGCCTAATGT CAATGCTGCA GTGAGGCCAC TCCCAGCTCT GAAAGAGAAT 
GTGAAGCGTG TCATGTTCTA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_175274.1
CDS	183..3329
Translation	MVRKRRTDAPSEGGEGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGRGGRGYGQPPQQQQQYGGPQEYQGRGRGGPPHQGGRGGYGGGRGGGPSSGPPQRQSVPELHQATSPTYQAVSSQPTLSEVSPTQVPEPTVLAQQFEQLSVEQGAPSQAIQPIPSSSKAFKFPMRPGKGQSGKRCIVKANHFFAELPDKDLHHYDVTITPEVTSRGVNRAVMKQLVDNYRDSHLGSRLPAYDGRKSLYTAGPLPFNSKEFRINLLDEEVGAGGQRREREFKVVIKLVARADLHHLGMFLEGKQSDAPQEALQVLDIVLRELPTSRYIPVGRSFYSPDIGKKQSLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEANPVIQFVCDLLNRDISSRPLSDADRVKIKKALRGVKVEVTHRGNMRRKYRISGLTAVATRELTFPVDERNTQKSVVEYFHETYGFRIQHTQLPCLQVGNSNRPNYLPMEVCKIVEGQRYSKRLNERQITALLKVTCQRPIDREKDILQTVQLNDYAKDNYAQEFGIKISTSLASVEARILPPPWLKYHESGREGTCLPQVGQWNMMNKKMINGGTVNNWICINFSRQVQDNLARTFCQELAQMCYVSGMAFNPEPVLPPVSARPEQVEKVLKTRYHDATSKLSQGKEIDLLIVILPDNNGSLYGDLKRICETELGIVSQCCLTKHVFKMSKQYMANVALKINVKVGGRNTVLVDALSRRIPLVSDRPTIIFGADVTHPHPGEDSSPSIAAVVASQDWPEITKYAGLVCAQAHRQELIQDLFKEWKDPQKGVVTGGMIKELLIAFRRSTGHKPLRIIFYRDGVSEGQFYQVLLYELDAIRKACASLEAGYQPPVTFVVVQKRHHTRLFAQNHNDRHSVDRSGNILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMASGSMARGGGMAGRSTRGPNVNAAVRPLPALKENVKRVMFYC

Target ORF information:

RefSeq Version	NM_103737.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Stabilizer of iron transporter SufD / Polynucleotidyl transferase (AGO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_103737.4

ORF Insert Sequence:

ATGGTGAGAA AGAGAAGAAC GGATGCTCCA TCTGAAGGAG GTGAAGGCTC TGGGTCTCGT 
GAAGCTGGTC CAGTCTCAGG TGGTGGACGT GGTTCACAGC GAGGTGGTTT CCAGCAGGGA 
GGAGGACAAC ACCAAGGTGG AAGGGGTTAT ACTCCTCAAC CTCAACAGGG AGGTCGTGGT 
GGTCGTGGAT ATGGGCAACC ACCACAACAG CAACAACAGT ATGGAGGACC ACAAGAGTAC 
CAAGGAAGAG GAAGAGGAGG ACCTCCTCAT CAAGGAGGTC GAGGAGGGTA TGGCGGTGGC 
CGTGGAGGTG GACCTTCTTC TGGACCACCG CAGAGACAAT CAGTTCCCGA GCTGCATCAA 
GCTACCTCAC CTACTTATCA AGCGGTGTCT TCTCAGCCTA CACTGTCTGA GGTGAGTCCT 
ACCCAGGTAC CAGAACCTAC TGTTCTGGCT CAGCAATTTG AACAACTCTC TGTTGAACAA 
GGAGCTCCCA GTCAGGCAAT CCAGCCTATA CCTTCTTCTA GCAAGGCTTT CAAGTTTCCA 
ATGAGGCCTG GTAAAGGACA GAGTGGAAAG CGTTGCATTG TGAAGGCTAA CCATTTCTTT 
GCTGAACTGC CTGATAAGGA TTTGCACCAT TATGATGTTA CCATTACTCC GGAAGTTACA 
TCAAGGGGTG TCAATCGTGC TGTGATGAAA CAACTTGTTG ATAATTATCG TGATTCTCAC 
CTTGGAAGTC GTCTTCCAGC GTATGATGGT CGAAAAAGTC TTTACACTGC TGGTCCACTT 
CCCTTTAACT CCAAGGAGTT CAGAATCAAT CTTCTTGACG AAGAAGTAGG GGCTGGAGGT 
CAAAGACGAG AAAGGGAATT TAAAGTTGTG ATCAAGCTAG TTGCACGTGC TGATCTGCAT 
CACCTAGGAA TGTTTTTGGA GGGGAAACAA TCAGATGCCC CACAGGAAGC TCTGCAGGTT 
CTTGACATTG TTCTTCGTGA GCTGCCGACC TCTAGGTATA TTCCGGTGGG CCGGTCCTTT 
TATTCCCCTG ATATAGGAAA AAAACAATCA TTGGGGGATG GCTTGGAGAG CTGGCGTGGA 
TTCTACCAAA GCATTCGTCC TACACAGATG GGCTTATCAC TCAATATTGA TATGTCATCG 
ACAGCCTTCA TAGAGGCAAA CCCTGTGATT CAGTTTGTCT GTGATTTGCT TAACCGGGAT 
ATTTCTTCTC GACCTTTATC TGATGCTGAT CGTGTTAAGA TAAAAAAGGC TCTTAGAGGT 
GTCAAAGTTG AAGTGACTCA TCGAGGAAAC ATGCGCCGGA AGTACCGCAT TTCCGGTTTG 
ACTGCTGTGG CCACTCGGGA ATTGACATTC CCAGTAGATG AAAGAAATAC TCAGAAATCT 
GTTGTAGAAT ACTTCCACGA AACATATGGT TTTCGCATTC AGCACACTCA ACTACCATGC 
TTGCAAGTTG GGAATTCTAA TAGGCCTAAT TACTTACCAA TGGAGGTATG CAAGATTGTT 
GAAGGCCAGC GGTATTCCAA AAGATTGAAT GAGAGACAGA TCACTGCTTT GCTGAAGGTT 
ACCTGTCAGC GCCCGATAGA TCGAGAAAAA GATATCTTAC AGACGGTGCA ACTCAATGAT 
TATGCTAAAG ATAATTATGC TCAAGAGTTT GGCATCAAAA TAAGTACTTC TCTGGCTTCT 
GTTGAGGCTC GTATACTGCC TCCTCCATGG CTTAAGTACC ACGAGTCTGG AAGGGAAGGG 
ACTTGTCTGC CACAAGTTGG TCAATGGAAC ATGATGAATA AGAAAATGAT CAATGGTGGA 
ACGGTGAATA ATTGGATCTG CATCAACTTT TCTAGGCAAG TGCAGGACAA TCTAGCGCGT 
ACATTTTGTC AGGAACTTGC TCAAATGTGT TACGTATCTG GCATGGCATT TAATCCGGAA 
CCAGTCCTCC CACCAGTCAG TGCTCGCCCT GAGCAAGTAG AGAAGGTCTT GAAGACTAGA 
TATCATGATG CCACATCAAA ACTCTCCCAA GGAAAAGAAA TTGATCTGCT TATTGTCATT 
CTGCCCGATA ATAATGGATC ATTATACGGT GATTTGAAAC GCATATGTGA GACTGAACTC 
GGCATAGTCT CTCAATGTTG CCTGACAAAG CATGTCTTTA AGATGAGCAA ACAATACATG 
GCTAATGTTG CGCTGAAGAT TAATGTGAAG GTTGGAGGAA GAAACACAGT GCTTGTTGAT 
GCTCTATCTA GGCGGATTCC TCTAGTCAGT GATCGACCCA CCATTATATT TGGTGCTGAT 
GTTACCCACC CTCACCCTGG AGAGGATTCA AGCCCATCTA TTGCTGCTGT TGTGGCATCT 
CAGGATTGGC CTGAAATCAC TAAATATGCT GGATTAGTTT GCGCTCAAGC GCATAGGCAG 
GAGCTCATTC AGGATCTGTT CAAAGAGTGG AAGGATCCTC AGAAAGGTGT GGTGACTGGT 
GGCATGATAA AGGAGTTGCT CATAGCCTTC CGTAGATCAA CTGGGCATAA ACCACTAAGG 
ATCATCTTCT ACAGGGATGG AGTCAGTGAG GGACAATTTT ACCAAGTTTT GCTCTATGAA 
CTTGATGCCA TCCGCAAGGC CTGTGCTTCG CTGGAAGCAG GTTATCAACC ACCAGTGACA 
TTTGTGGTGG TGCAGAAGCG TCATCACACG AGGCTGTTTG CTCAGAACCA CAATGATCGC 
CATTCGGTGG ACAGAAGTGG GAATATTTTA CCTGGCACTG TTGTGGACTC TAAAATCTGC 
CACCCTACAG AGTTTGACTT TTACCTCTGT AGTCATGCTG GTATTCAGGG CACTTCTCGA 
CCTGCTCATT ACCACGTTCT TTGGGATGAG AACAACTTTA CTGCAGATGG ACTTCAATCT 
CTGACCAATA ACTTATGTTA CACGTATGCA AGATGCACAC GCTCAGTTTC AATTGTTCCC 
CCTGCATATT ATGCACATCT AGCAGCTTTT AGGGCTCGAT TCTACATGGA GCCAGAGACA 
TCAGACAGTG GCTCAATGGC TAGTGGGAGC ATGGCACGTG GAGGTGGAAT GGCTGGTAGA 
AGCACACGCG GGCCTAATGT CAATGCTGCA GTGAGGCCAC TCCCAGCTCT GAAAGAGAAT 
GTGAAGCGTG TCATGTTCTA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO1 ( NM_179453.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_849784.1 Arabidopsis thaliana Stabilizer of iron transporter SufD / Polynucleotidyl transferase (AGO1), mRNA.

Price & Availability↑

CloneID	OAb25830
Clone ID Related Accession (Same CDS sequence)	NM_179453.3 , NM_001198240.1
Accession Version	NM_179453.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Stabilizer of iron transporter SufD / Polynucleotidyl transferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_179453.2.

ATGGTGAGAA AGAGAAGAAC GGATGCTCCA TCTGAAGGAG GTGAAGGCTC TGGGTCTCGT 
GAAGCTGGTC CAGTCTCAGG TGGTGGACGT GGTTCACAGC GAGGTGGTTT CCAGCAGGGA 
GGAGGACAAC ACCAAGGTGG AAGGGGTTAT ACTCCTCAAC CTCAACAGGG AGGTCGTGGT 
GGTCGTGGAT ATGGGCAACC ACCACAACAG CAACAACAGT ATGGAGGACC ACAAGAGTAC 
CAAGGAAGAG GAAGAGGAGG ACCTCCTCAT CAAGGAGGTC GAGGAGGGTA TGGCGGTGGC 
CGTGGAGGTG GACCTTCTTC TGGACCACCG CAGAGACAAT CAGTTCCCGA GCTGCATCAA 
GCTACCTCAC CTACTTATCA AGCGGTGTCT TCTCAGCCTA CACTGTCTGA GGTGAGTCCT 
ACCCAGGTAC CAGAACCTAC TGTTCTGGCT CAGCAATTTG AACAACTCTC TGTTGAACAA 
GGAGCTCCCA GTCAGGCAAT CCAGCCTATA CCTTCTTCTA GCAAGGCTTT CAAGTTTCCA 
ATGAGGCCTG GTAAAGGACA GAGTGGAAAG CGTTGCATTG TGAAGGCTAA CCATTTCTTT 
GCTGAACTGC CTGATAAGGA TTTGCACCAT TATGATGTTA CCATTACTCC GGAAGTTACA 
TCAAGGGGTG TCAATCGTGC TGTGATGAAA CAACTTGTTG ATAATTATCG TGATTCTCAC 
CTTGGAAGTC GTCTTCCAGC GTATGATGGT CGAAAAAGTC TTTACACTGC TGGTCCACTT 
CCCTTTAACT CCAAGGAGTT CAGAATCAAT CTTCTTGACG AAGAAGTAGG GGCTGGAGGT 
CAAAGACGAG AAAGGGAATT TAAAGTTGTG ATCAAGCTAG TTGCACGTGC TGATCTGCAT 
CACCTAGGAA TGTTTTTGGA GGGGAAACAA TCAGATGCCC CACAGGAAGC TCTGCAGGTT 
CTTGACATTG TTCTTCGTGA GCTGCCGACC TCTAGAATCA GGTATATTCC GGTGGGCCGG 
TCCTTTTATT CCCCTGATAT AGGAAAAAAA CAATCATTGG GGGATGGCTT GGAGAGCTGG 
CGTGGATTCT ACCAAAGCAT TCGTCCTACA CAGATGGGCT TATCACTCAA TATTGATATG 
TCATCGACAG CCTTCATAGA GGCAAACCCT GTGATTCAGT TTGTCTGTGA TTTGCTTAAC 
CGGGATATTT CTTCTCGACC TTTATCTGAT GCTGATCGTG TTAAGATAAA AAAGGCTCTT 
AGAGGTGTCA AAGTTGAAGT GACTCATCGA GGAAACATGC GCCGGAAGTA CCGCATTTCC 
GGTTTGACTG CTGTGGCCAC TCGGGAATTG ACATTCCCAG TAGATGAAAG AAATACTCAG 
AAATCTGTTG TAGAATACTT CCACGAAACA TATGGTTTTC GCATTCAGCA CACTCAACTA 
CCATGCTTGC AAGTTGGGAA TTCTAATAGG CCTAATTACT TACCAATGGA GGTATGCAAG 
ATTGTTGAAG GCCAGCGGTA TTCCAAAAGA TTGAATGAGA GACAGATCAC TGCTTTGCTG 
AAGGTTACCT GTCAGCGCCC GATAGATCGA GAAAAAGATA TCTTACAGAC GGTGCAACTC 
AATGATTATG CTAAAGATAA TTATGCTCAA GAGTTTGGCA TCAAAATAAG TACTTCTCTG 
GCTTCTGTTG AGGCTCGTAT ACTGCCTCCT CCATGGCTTA AGTACCACGA GTCTGGAAGG 
GAAGGGACTT GTCTGCCACA AGTTGGTCAA TGGAACATGA TGAATAAGAA AATGATCAAT 
GGTGGAACGG TGAATAATTG GATCTGCATC AACTTTTCTA GGCAAGTGCA GGACAATCTA 
GCGCGTACAT TTTGTCAGGA ACTTGCTCAA ATGTGTTACG TATCTGGCAT GGCATTTAAT 
CCGGAACCAG TCCTCCCACC AGTCAGTGCT CGCCCTGAGC AAGTAGAGAA GGTCTTGAAG 
ACTAGATATC ATGATGCCAC ATCAAAACTC TCCCAAGGAA AAGAAATTGA TCTGCTTATT 
GTCATTCTGC CCGATAATAA TGGATCATTA TACGGTGATT TGAAACGCAT ATGTGAGACT 
GAACTCGGCA TAGTCTCTCA ATGTTGCCTG ACAAAGCATG TCTTTAAGAT GAGCAAACAA 
TACATGGCTA ATGTTGCGCT GAAGATTAAT GTGAAGGTTG GAGGAAGAAA CACAGTGCTT 
GTTGATGCTC TATCTAGGCG GATTCCTCTA GTCAGTGATC GACCCACCAT TATATTTGGT 
GCTGATGTTA CCCACCCTCA CCCTGGAGAG GATTCAAGCC CATCTATTGC TGCTGTTGTG 
GCATCTCAGG ATTGGCCTGA AATCACTAAA TATGCTGGAT TAGTTTGCGC TCAAGCGCAT 
AGGCAGGAGC TCATTCAGGA TCTGTTCAAA GAGTGGAAGG ATCCTCAGAA AGGTGTGGTG 
ACTGGTGGCA TGATAAAGGA GTTGCTCATA GCCTTCCGTA GATCAACTGG GCATAAACCA 
CTAAGGATCA TCTTCTACAG GGATGGAGTC AGTGAGGGAC AATTTTACCA AGTTTTGCTC 
TATGAACTTG ATGCCATCCG CAAGGCCTGT GCTTCGCTGG AAGCAGGTTA TCAACCACCA 
GTGACATTTG TGGTGGTGCA GAAGCGTCAT CACACGAGGC TGTTTGCTCA GAACCACAAT 
GATCGCCATT CGGTGGACAG AAGTGGGAAT ATTTTACCTG GCACTGTTGT GGACTCTAAA 
ATCTGCCACC CTACAGAGTT TGACTTTTAC CTCTGTAGTC ATGCTGGTAT TCAGGGCACT 
TCTCGACCTG CTCATTACCA CGTTCTTTGG GATGAGAACA ACTTTACTGC AGATGGACTT 
CAATCTCTGA CCAATAACTT ATGTTACACG TATGCAAGAT GCACACGCTC AGTTTCAATT 
GTTCCCCCTG CATATTATGC ACATCTAGCA GCTTTTAGGG CTCGATTCTA CATGGAGCCA 
GAGACATCAG ACAGTGGCTC AATGGCTAGT GGGAGCATGG CACGTGGAGG TGGAATGGCT 
GGTAGAAGCA CACGCGGGCC TAATGTCAAT GCTGCAGTGA GGCCACTCCC AGCTCTGAAA 
GAGAATGTGA AGCGTGTCAT GTTCTACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849784.1
CDS	214..3366
Translation	MVRKRRTDAPSEGGEGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGRGGRGYGQPPQQQQQYGGPQEYQGRGRGGPPHQGGRGGYGGGRGGGPSSGPPQRQSVPELHQATSPTYQAVSSQPTLSEVSPTQVPEPTVLAQQFEQLSVEQGAPSQAIQPIPSSSKAFKFPMRPGKGQSGKRCIVKANHFFAELPDKDLHHYDVTITPEVTSRGVNRAVMKQLVDNYRDSHLGSRLPAYDGRKSLYTAGPLPFNSKEFRINLLDEEVGAGGQRREREFKVVIKLVARADLHHLGMFLEGKQSDAPQEALQVLDIVLRELPTSRIRYIPVGRSFYSPDIGKKQSLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEANPVIQFVCDLLNRDISSRPLSDADRVKIKKALRGVKVEVTHRGNMRRKYRISGLTAVATRELTFPVDERNTQKSVVEYFHETYGFRIQHTQLPCLQVGNSNRPNYLPMEVCKIVEGQRYSKRLNERQITALLKVTCQRPIDREKDILQTVQLNDYAKDNYAQEFGIKISTSLASVEARILPPPWLKYHESGREGTCLPQVGQWNMMNKKMINGGTVNNWICINFSRQVQDNLARTFCQELAQMCYVSGMAFNPEPVLPPVSARPEQVEKVLKTRYHDATSKLSQGKEIDLLIVILPDNNGSLYGDLKRICETELGIVSQCCLTKHVFKMSKQYMANVALKINVKVGGRNTVLVDALSRRIPLVSDRPTIIFGADVTHPHPGEDSSPSIAAVVASQDWPEITKYAGLVCAQAHRQELIQDLFKEWKDPQKGVVTGGMIKELLIAFRRSTGHKPLRIIFYRDGVSEGQFYQVLLYELDAIRKACASLEAGYQPPVTFVVVQKRHHTRLFAQNHNDRHSVDRSGNILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMASGSMARGGGMAGRSTRGPNVNAAVRPLPALKENVKRVMFYC

Target ORF information:

RefSeq Version	NM_179453.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Stabilizer of iron transporter SufD / Polynucleotidyl transferase (AGO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_179453.3

ORF Insert Sequence:

ATGGTGAGAA AGAGAAGAAC GGATGCTCCA TCTGAAGGAG GTGAAGGCTC TGGGTCTCGT 
GAAGCTGGTC CAGTCTCAGG TGGTGGACGT GGTTCACAGC GAGGTGGTTT CCAGCAGGGA 
GGAGGACAAC ACCAAGGTGG AAGGGGTTAT ACTCCTCAAC CTCAACAGGG AGGTCGTGGT 
GGTCGTGGAT ATGGGCAACC ACCACAACAG CAACAACAGT ATGGAGGACC ACAAGAGTAC 
CAAGGAAGAG GAAGAGGAGG ACCTCCTCAT CAAGGAGGTC GAGGAGGGTA TGGCGGTGGC 
CGTGGAGGTG GACCTTCTTC TGGACCACCG CAGAGACAAT CAGTTCCCGA GCTGCATCAA 
GCTACCTCAC CTACTTATCA AGCGGTGTCT TCTCAGCCTA CACTGTCTGA GGTGAGTCCT 
ACCCAGGTAC CAGAACCTAC TGTTCTGGCT CAGCAATTTG AACAACTCTC TGTTGAACAA 
GGAGCTCCCA GTCAGGCAAT CCAGCCTATA CCTTCTTCTA GCAAGGCTTT CAAGTTTCCA 
ATGAGGCCTG GTAAAGGACA GAGTGGAAAG CGTTGCATTG TGAAGGCTAA CCATTTCTTT 
GCTGAACTGC CTGATAAGGA TTTGCACCAT TATGATGTTA CCATTACTCC GGAAGTTACA 
TCAAGGGGTG TCAATCGTGC TGTGATGAAA CAACTTGTTG ATAATTATCG TGATTCTCAC 
CTTGGAAGTC GTCTTCCAGC GTATGATGGT CGAAAAAGTC TTTACACTGC TGGTCCACTT 
CCCTTTAACT CCAAGGAGTT CAGAATCAAT CTTCTTGACG AAGAAGTAGG GGCTGGAGGT 
CAAAGACGAG AAAGGGAATT TAAAGTTGTG ATCAAGCTAG TTGCACGTGC TGATCTGCAT 
CACCTAGGAA TGTTTTTGGA GGGGAAACAA TCAGATGCCC CACAGGAAGC TCTGCAGGTT 
CTTGACATTG TTCTTCGTGA GCTGCCGACC TCTAGAATCA GGTATATTCC GGTGGGCCGG 
TCCTTTTATT CCCCTGATAT AGGAAAAAAA CAATCATTGG GGGATGGCTT GGAGAGCTGG 
CGTGGATTCT ACCAAAGCAT TCGTCCTACA CAGATGGGCT TATCACTCAA TATTGATATG 
TCATCGACAG CCTTCATAGA GGCAAACCCT GTGATTCAGT TTGTCTGTGA TTTGCTTAAC 
CGGGATATTT CTTCTCGACC TTTATCTGAT GCTGATCGTG TTAAGATAAA AAAGGCTCTT 
AGAGGTGTCA AAGTTGAAGT GACTCATCGA GGAAACATGC GCCGGAAGTA CCGCATTTCC 
GGTTTGACTG CTGTGGCCAC TCGGGAATTG ACATTCCCAG TAGATGAAAG AAATACTCAG 
AAATCTGTTG TAGAATACTT CCACGAAACA TATGGTTTTC GCATTCAGCA CACTCAACTA 
CCATGCTTGC AAGTTGGGAA TTCTAATAGG CCTAATTACT TACCAATGGA GGTATGCAAG 
ATTGTTGAAG GCCAGCGGTA TTCCAAAAGA TTGAATGAGA GACAGATCAC TGCTTTGCTG 
AAGGTTACCT GTCAGCGCCC GATAGATCGA GAAAAAGATA TCTTACAGAC GGTGCAACTC 
AATGATTATG CTAAAGATAA TTATGCTCAA GAGTTTGGCA TCAAAATAAG TACTTCTCTG 
GCTTCTGTTG AGGCTCGTAT ACTGCCTCCT CCATGGCTTA AGTACCACGA GTCTGGAAGG 
GAAGGGACTT GTCTGCCACA AGTTGGTCAA TGGAACATGA TGAATAAGAA AATGATCAAT 
GGTGGAACGG TGAATAATTG GATCTGCATC AACTTTTCTA GGCAAGTGCA GGACAATCTA 
GCGCGTACAT TTTGTCAGGA ACTTGCTCAA ATGTGTTACG TATCTGGCAT GGCATTTAAT 
CCGGAACCAG TCCTCCCACC AGTCAGTGCT CGCCCTGAGC AAGTAGAGAA GGTCTTGAAG 
ACTAGATATC ATGATGCCAC ATCAAAACTC TCCCAAGGAA AAGAAATTGA TCTGCTTATT 
GTCATTCTGC CCGATAATAA TGGATCATTA TACGGTGATT TGAAACGCAT ATGTGAGACT 
GAACTCGGCA TAGTCTCTCA ATGTTGCCTG ACAAAGCATG TCTTTAAGAT GAGCAAACAA 
TACATGGCTA ATGTTGCGCT GAAGATTAAT GTGAAGGTTG GAGGAAGAAA CACAGTGCTT 
GTTGATGCTC TATCTAGGCG GATTCCTCTA GTCAGTGATC GACCCACCAT TATATTTGGT 
GCTGATGTTA CCCACCCTCA CCCTGGAGAG GATTCAAGCC CATCTATTGC TGCTGTTGTG 
GCATCTCAGG ATTGGCCTGA AATCACTAAA TATGCTGGAT TAGTTTGCGC TCAAGCGCAT 
AGGCAGGAGC TCATTCAGGA TCTGTTCAAA GAGTGGAAGG ATCCTCAGAA AGGTGTGGTG 
ACTGGTGGCA TGATAAAGGA GTTGCTCATA GCCTTCCGTA GATCAACTGG GCATAAACCA 
CTAAGGATCA TCTTCTACAG GGATGGAGTC AGTGAGGGAC AATTTTACCA AGTTTTGCTC 
TATGAACTTG ATGCCATCCG CAAGGCCTGT GCTTCGCTGG AAGCAGGTTA TCAACCACCA 
GTGACATTTG TGGTGGTGCA GAAGCGTCAT CACACGAGGC TGTTTGCTCA GAACCACAAT 
GATCGCCATT CGGTGGACAG AAGTGGGAAT ATTTTACCTG GCACTGTTGT GGACTCTAAA 
ATCTGCCACC CTACAGAGTT TGACTTTTAC CTCTGTAGTC ATGCTGGTAT TCAGGGCACT 
TCTCGACCTG CTCATTACCA CGTTCTTTGG GATGAGAACA ACTTTACTGC AGATGGACTT 
CAATCTCTGA CCAATAACTT ATGTTACACG TATGCAAGAT GCACACGCTC AGTTTCAATT 
GTTCCCCCTG CATATTATGC ACATCTAGCA GCTTTTAGGG CTCGATTCTA CATGGAGCCA 
GAGACATCAG ACAGTGGCTC AATGGCTAGT GGGAGCATGG CACGTGGAGG TGGAATGGCT 
GGTAGAAGCA CACGCGGGCC TAATGTCAAT GCTGCAGTGA GGCCACTCCC AGCTCTGAAA 
GAGAATGTGA AGCGTGTCAT GTTCTACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO1 ( NM_001198240.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001185169.1 Arabidopsis thaliana Stabilizer of iron transporter SufD / Polynucleotidyl transferase (AGO1), mRNA.

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CloneID	OAb25830
Clone ID Related Accession (Same CDS sequence)	NM_179453.3 , NM_001198240.1
Accession Version	NM_001198240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Stabilizer of iron transporter SufD / Polynucleotidyl transferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGTGAGAA AGAGAAGAAC GGATGCTCCA TCTGAAGGAG GTGAAGGCTC TGGGTCTCGT 
GAAGCTGGTC CAGTCTCAGG TGGTGGACGT GGTTCACAGC GAGGTGGTTT CCAGCAGGGA 
GGAGGACAAC ACCAAGGTGG AAGGGGTTAT ACTCCTCAAC CTCAACAGGG AGGTCGTGGT 
GGTCGTGGAT ATGGGCAACC ACCACAACAG CAACAACAGT ATGGAGGACC ACAAGAGTAC 
CAAGGAAGAG GAAGAGGAGG ACCTCCTCAT CAAGGAGGTC GAGGAGGGTA TGGCGGTGGC 
CGTGGAGGTG GACCTTCTTC TGGACCACCG CAGAGACAAT CAGTTCCCGA GCTGCATCAA 
GCTACCTCAC CTACTTATCA AGCGGTGTCT TCTCAGCCTA CACTGTCTGA GGTGAGTCCT 
ACCCAGGTAC CAGAACCTAC TGTTCTGGCT CAGCAATTTG AACAACTCTC TGTTGAACAA 
GGAGCTCCCA GTCAGGCAAT CCAGCCTATA CCTTCTTCTA GCAAGGCTTT CAAGTTTCCA 
ATGAGGCCTG GTAAAGGACA GAGTGGAAAG CGTTGCATTG TGAAGGCTAA CCATTTCTTT 
GCTGAACTGC CTGATAAGGA TTTGCACCAT TATGATGTTA CCATTACTCC GGAAGTTACA 
TCAAGGGGTG TCAATCGTGC TGTGATGAAA CAACTTGTTG ATAATTATCG TGATTCTCAC 
CTTGGAAGTC GTCTTCCAGC GTATGATGGT CGAAAAAGTC TTTACACTGC TGGTCCACTT 
CCCTTTAACT CCAAGGAGTT CAGAATCAAT CTTCTTGACG AAGAAGTAGG GGCTGGAGGT 
CAAAGACGAG AAAGGGAATT TAAAGTTGTG ATCAAGCTAG TTGCACGTGC TGATCTGCAT 
CACCTAGGAA TGTTTTTGGA GGGGAAACAA TCAGATGCCC CACAGGAAGC TCTGCAGGTT 
CTTGACATTG TTCTTCGTGA GCTGCCGACC TCTAGAATCA GGTATATTCC GGTGGGCCGG 
TCCTTTTATT CCCCTGATAT AGGAAAAAAA CAATCATTGG GGGATGGCTT GGAGAGCTGG 
CGTGGATTCT ACCAAAGCAT TCGTCCTACA CAGATGGGCT TATCACTCAA TATTGATATG 
TCATCGACAG CCTTCATAGA GGCAAACCCT GTGATTCAGT TTGTCTGTGA TTTGCTTAAC 
CGGGATATTT CTTCTCGACC TTTATCTGAT GCTGATCGTG TTAAGATAAA AAAGGCTCTT 
AGAGGTGTCA AAGTTGAAGT GACTCATCGA GGAAACATGC GCCGGAAGTA CCGCATTTCC 
GGTTTGACTG CTGTGGCCAC TCGGGAATTG ACATTCCCAG TAGATGAAAG AAATACTCAG 
AAATCTGTTG TAGAATACTT CCACGAAACA TATGGTTTTC GCATTCAGCA CACTCAACTA 
CCATGCTTGC AAGTTGGGAA TTCTAATAGG CCTAATTACT TACCAATGGA GGTATGCAAG 
ATTGTTGAAG GCCAGCGGTA TTCCAAAAGA TTGAATGAGA GACAGATCAC TGCTTTGCTG 
AAGGTTACCT GTCAGCGCCC GATAGATCGA GAAAAAGATA TCTTACAGAC GGTGCAACTC 
AATGATTATG CTAAAGATAA TTATGCTCAA GAGTTTGGCA TCAAAATAAG TACTTCTCTG 
GCTTCTGTTG AGGCTCGTAT ACTGCCTCCT CCATGGCTTA AGTACCACGA GTCTGGAAGG 
GAAGGGACTT GTCTGCCACA AGTTGGTCAA TGGAACATGA TGAATAAGAA AATGATCAAT 
GGTGGAACGG TGAATAATTG GATCTGCATC AACTTTTCTA GGCAAGTGCA GGACAATCTA 
GCGCGTACAT TTTGTCAGGA ACTTGCTCAA ATGTGTTACG TATCTGGCAT GGCATTTAAT 
CCGGAACCAG TCCTCCCACC AGTCAGTGCT CGCCCTGAGC AAGTAGAGAA GGTCTTGAAG 
ACTAGATATC ATGATGCCAC ATCAAAACTC TCCCAAGGAA AAGAAATTGA TCTGCTTATT 
GTCATTCTGC CCGATAATAA TGGATCATTA TACGGTGATT TGAAACGCAT ATGTGAGACT 
GAACTCGGCA TAGTCTCTCA ATGTTGCCTG ACAAAGCATG TCTTTAAGAT GAGCAAACAA 
TACATGGCTA ATGTTGCGCT GAAGATTAAT GTGAAGGTTG GAGGAAGAAA CACAGTGCTT 
GTTGATGCTC TATCTAGGCG GATTCCTCTA GTCAGTGATC GACCCACCAT TATATTTGGT 
GCTGATGTTA CCCACCCTCA CCCTGGAGAG GATTCAAGCC CATCTATTGC TGCTGTTGTG 
GCATCTCAGG ATTGGCCTGA AATCACTAAA TATGCTGGAT TAGTTTGCGC TCAAGCGCAT 
AGGCAGGAGC TCATTCAGGA TCTGTTCAAA GAGTGGAAGG ATCCTCAGAA AGGTGTGGTG 
ACTGGTGGCA TGATAAAGGA GTTGCTCATA GCCTTCCGTA GATCAACTGG GCATAAACCA 
CTAAGGATCA TCTTCTACAG GGATGGAGTC AGTGAGGGAC AATTTTACCA AGTTTTGCTC 
TATGAACTTG ATGCCATCCG CAAGGCCTGT GCTTCGCTGG AAGCAGGTTA TCAACCACCA 
GTGACATTTG TGGTGGTGCA GAAGCGTCAT CACACGAGGC TGTTTGCTCA GAACCACAAT 
GATCGCCATT CGGTGGACAG AAGTGGGAAT ATTTTACCTG GCACTGTTGT GGACTCTAAA 
ATCTGCCACC CTACAGAGTT TGACTTTTAC CTCTGTAGTC ATGCTGGTAT TCAGGGCACT 
TCTCGACCTG CTCATTACCA CGTTCTTTGG GATGAGAACA ACTTTACTGC AGATGGACTT 
CAATCTCTGA CCAATAACTT ATGTTACACG TATGCAAGAT GCACACGCTC AGTTTCAATT 
GTTCCCCCTG CATATTATGC ACATCTAGCA GCTTTTAGGG CTCGATTCTA CATGGAGCCA 
GAGACATCAG ACAGTGGCTC AATGGCTAGT GGGAGCATGG CACGTGGAGG TGGAATGGCT 
GGTAGAAGCA CACGCGGGCC TAATGTCAAT GCTGCAGTGA GGCCACTCCC AGCTCTGAAA 
GAGAATGTGA AGCGTGTCAT GTTCTACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185169.1
CDS	178..3330
Translation	MVRKRRTDAPSEGGEGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGRGGRGYGQPPQQQQQYGGPQEYQGRGRGGPPHQGGRGGYGGGRGGGPSSGPPQRQSVPELHQATSPTYQAVSSQPTLSEVSPTQVPEPTVLAQQFEQLSVEQGAPSQAIQPIPSSSKAFKFPMRPGKGQSGKRCIVKANHFFAELPDKDLHHYDVTITPEVTSRGVNRAVMKQLVDNYRDSHLGSRLPAYDGRKSLYTAGPLPFNSKEFRINLLDEEVGAGGQRREREFKVVIKLVARADLHHLGMFLEGKQSDAPQEALQVLDIVLRELPTSRIRYIPVGRSFYSPDIGKKQSLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEANPVIQFVCDLLNRDISSRPLSDADRVKIKKALRGVKVEVTHRGNMRRKYRISGLTAVATRELTFPVDERNTQKSVVEYFHETYGFRIQHTQLPCLQVGNSNRPNYLPMEVCKIVEGQRYSKRLNERQITALLKVTCQRPIDREKDILQTVQLNDYAKDNYAQEFGIKISTSLASVEARILPPPWLKYHESGREGTCLPQVGQWNMMNKKMINGGTVNNWICINFSRQVQDNLARTFCQELAQMCYVSGMAFNPEPVLPPVSARPEQVEKVLKTRYHDATSKLSQGKEIDLLIVILPDNNGSLYGDLKRICETELGIVSQCCLTKHVFKMSKQYMANVALKINVKVGGRNTVLVDALSRRIPLVSDRPTIIFGADVTHPHPGEDSSPSIAAVVASQDWPEITKYAGLVCAQAHRQELIQDLFKEWKDPQKGVVTGGMIKELLIAFRRSTGHKPLRIIFYRDGVSEGQFYQVLLYELDAIRKACASLEAGYQPPVTFVVVQKRHHTRLFAQNHNDRHSVDRSGNILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMASGSMARGGGMAGRSTRGPNVNAAVRPLPALKENVKRVMFYC

Target ORF information:

RefSeq Version	NM_001198240.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Stabilizer of iron transporter SufD / Polynucleotidyl transferase (AGO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198240.1

ORF Insert Sequence:

ATGGTGAGAA AGAGAAGAAC GGATGCTCCA TCTGAAGGAG GTGAAGGCTC TGGGTCTCGT 
GAAGCTGGTC CAGTCTCAGG TGGTGGACGT GGTTCACAGC GAGGTGGTTT CCAGCAGGGA 
GGAGGACAAC ACCAAGGTGG AAGGGGTTAT ACTCCTCAAC CTCAACAGGG AGGTCGTGGT 
GGTCGTGGAT ATGGGCAACC ACCACAACAG CAACAACAGT ATGGAGGACC ACAAGAGTAC 
CAAGGAAGAG GAAGAGGAGG ACCTCCTCAT CAAGGAGGTC GAGGAGGGTA TGGCGGTGGC 
CGTGGAGGTG GACCTTCTTC TGGACCACCG CAGAGACAAT CAGTTCCCGA GCTGCATCAA 
GCTACCTCAC CTACTTATCA AGCGGTGTCT TCTCAGCCTA CACTGTCTGA GGTGAGTCCT 
ACCCAGGTAC CAGAACCTAC TGTTCTGGCT CAGCAATTTG AACAACTCTC TGTTGAACAA 
GGAGCTCCCA GTCAGGCAAT CCAGCCTATA CCTTCTTCTA GCAAGGCTTT CAAGTTTCCA 
ATGAGGCCTG GTAAAGGACA GAGTGGAAAG CGTTGCATTG TGAAGGCTAA CCATTTCTTT 
GCTGAACTGC CTGATAAGGA TTTGCACCAT TATGATGTTA CCATTACTCC GGAAGTTACA 
TCAAGGGGTG TCAATCGTGC TGTGATGAAA CAACTTGTTG ATAATTATCG TGATTCTCAC 
CTTGGAAGTC GTCTTCCAGC GTATGATGGT CGAAAAAGTC TTTACACTGC TGGTCCACTT 
CCCTTTAACT CCAAGGAGTT CAGAATCAAT CTTCTTGACG AAGAAGTAGG GGCTGGAGGT 
CAAAGACGAG AAAGGGAATT TAAAGTTGTG ATCAAGCTAG TTGCACGTGC TGATCTGCAT 
CACCTAGGAA TGTTTTTGGA GGGGAAACAA TCAGATGCCC CACAGGAAGC TCTGCAGGTT 
CTTGACATTG TTCTTCGTGA GCTGCCGACC TCTAGAATCA GGTATATTCC GGTGGGCCGG 
TCCTTTTATT CCCCTGATAT AGGAAAAAAA CAATCATTGG GGGATGGCTT GGAGAGCTGG 
CGTGGATTCT ACCAAAGCAT TCGTCCTACA CAGATGGGCT TATCACTCAA TATTGATATG 
TCATCGACAG CCTTCATAGA GGCAAACCCT GTGATTCAGT TTGTCTGTGA TTTGCTTAAC 
CGGGATATTT CTTCTCGACC TTTATCTGAT GCTGATCGTG TTAAGATAAA AAAGGCTCTT 
AGAGGTGTCA AAGTTGAAGT GACTCATCGA GGAAACATGC GCCGGAAGTA CCGCATTTCC 
GGTTTGACTG CTGTGGCCAC TCGGGAATTG ACATTCCCAG TAGATGAAAG AAATACTCAG 
AAATCTGTTG TAGAATACTT CCACGAAACA TATGGTTTTC GCATTCAGCA CACTCAACTA 
CCATGCTTGC AAGTTGGGAA TTCTAATAGG CCTAATTACT TACCAATGGA GGTATGCAAG 
ATTGTTGAAG GCCAGCGGTA TTCCAAAAGA TTGAATGAGA GACAGATCAC TGCTTTGCTG 
AAGGTTACCT GTCAGCGCCC GATAGATCGA GAAAAAGATA TCTTACAGAC GGTGCAACTC 
AATGATTATG CTAAAGATAA TTATGCTCAA GAGTTTGGCA TCAAAATAAG TACTTCTCTG 
GCTTCTGTTG AGGCTCGTAT ACTGCCTCCT CCATGGCTTA AGTACCACGA GTCTGGAAGG 
GAAGGGACTT GTCTGCCACA AGTTGGTCAA TGGAACATGA TGAATAAGAA AATGATCAAT 
GGTGGAACGG TGAATAATTG GATCTGCATC AACTTTTCTA GGCAAGTGCA GGACAATCTA 
GCGCGTACAT TTTGTCAGGA ACTTGCTCAA ATGTGTTACG TATCTGGCAT GGCATTTAAT 
CCGGAACCAG TCCTCCCACC AGTCAGTGCT CGCCCTGAGC AAGTAGAGAA GGTCTTGAAG 
ACTAGATATC ATGATGCCAC ATCAAAACTC TCCCAAGGAA AAGAAATTGA TCTGCTTATT 
GTCATTCTGC CCGATAATAA TGGATCATTA TACGGTGATT TGAAACGCAT ATGTGAGACT 
GAACTCGGCA TAGTCTCTCA ATGTTGCCTG ACAAAGCATG TCTTTAAGAT GAGCAAACAA 
TACATGGCTA ATGTTGCGCT GAAGATTAAT GTGAAGGTTG GAGGAAGAAA CACAGTGCTT 
GTTGATGCTC TATCTAGGCG GATTCCTCTA GTCAGTGATC GACCCACCAT TATATTTGGT 
GCTGATGTTA CCCACCCTCA CCCTGGAGAG GATTCAAGCC CATCTATTGC TGCTGTTGTG 
GCATCTCAGG ATTGGCCTGA AATCACTAAA TATGCTGGAT TAGTTTGCGC TCAAGCGCAT 
AGGCAGGAGC TCATTCAGGA TCTGTTCAAA GAGTGGAAGG ATCCTCAGAA AGGTGTGGTG 
ACTGGTGGCA TGATAAAGGA GTTGCTCATA GCCTTCCGTA GATCAACTGG GCATAAACCA 
CTAAGGATCA TCTTCTACAG GGATGGAGTC AGTGAGGGAC AATTTTACCA AGTTTTGCTC 
TATGAACTTG ATGCCATCCG CAAGGCCTGT GCTTCGCTGG AAGCAGGTTA TCAACCACCA 
GTGACATTTG TGGTGGTGCA GAAGCGTCAT CACACGAGGC TGTTTGCTCA GAACCACAAT 
GATCGCCATT CGGTGGACAG AAGTGGGAAT ATTTTACCTG GCACTGTTGT GGACTCTAAA 
ATCTGCCACC CTACAGAGTT TGACTTTTAC CTCTGTAGTC ATGCTGGTAT TCAGGGCACT 
TCTCGACCTG CTCATTACCA CGTTCTTTGG GATGAGAACA ACTTTACTGC AGATGGACTT 
CAATCTCTGA CCAATAACTT ATGTTACACG TATGCAAGAT GCACACGCTC AGTTTCAATT 
GTTCCCCCTG CATATTATGC ACATCTAGCA GCTTTTAGGG CTCGATTCTA CATGGAGCCA 
GAGACATCAG ACAGTGGCTC AATGGCTAGT GGGAGCATGG CACGTGGAGG TGGAATGGCT 
GGTAGAAGCA CACGCGGGCC TAATGTCAAT GCTGCAGTGA GGCCACTCCC AGCTCTGAAA 
GAGAATGTGA AGCGTGTCAT GTTCTACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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AGO1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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